The invention relates to a preparation method of night-blooming cereus nutrient fertilizer. The nutrient fertilizer is composed of the components in percentage by weight: pine needle powder, bulbus fritillariae cirrhosae powder, spatholobus stem powder, bean cake powder, bone meal, ferrous sulfate, carapace powder, peanut stem powder, dry pig manure, sesame straw powder, bean straw powder, wheat bran, calcium superphosphate, triadimefon, Bordeaux mixture, methamidophos, anilazine and trichlorfon. Due to various nutrients contained in the nutrient fertilizer, the grown night-blooming cereus is full, thick and tender in leaves, dense in growth, strong in growing ability, good light receiving, magnificent in crowns, and excellent in landscape effect, thus achieving the purpose of the invention.