13996 results about "Drug resistance" patented technology

The present invention provides methods for analyzing a combination of biomarkers to individualize tyrosine kinase inhibitor therapy in patients who have been diagnosed with cancer. In particular, the assay methods of the present invention are useful for predicting, identifying, or monitoring the response of a tumor, tumor cell, or patient to treatment with a tyrosine kinase inhibitor using an algorithm based upon biomarker profiling. The assay methods of the present invention are also useful for predicting whether a patient has a risk of developing toxicity or resistance to treatment with a tyrosine kinase inhibitor. In addition, the assay methods of the present invention are useful for monitoring tyrosine kinase inhibitor therapy in a patient receiving the drug to evaluate whether the patient will develop resistance to the drug. Furthermore, the assay methods of the present invention are useful for optimizing the dose of a tyrosine kinase inhibitor in a patient receiving the drug to achieve therapeutic efficacy and/or reduce toxic side-effects.

The invention discloses a bactericide pesticide composite containing oligochitosan, which comprises a component A and a component B, wherein the component A is oligochitosan or a derivative of the oligochitosan; and the component B is one or several of active compounds. The pesticide composition can produce a synergistic action, reduce the dosage of a chemically synthetic bactericide, prevent or delay the medicament resistance of the bactericide, improve crop stress resistance and yield, regulate metabolic balance in plant bodies and improve quality of agricultural products, improve the application safety of the bactericide and is more safe to ecological environmental and human bodies.

The subject invention includes methods and plants for controlling lepidopteran insects, said plants comprising a Vip3Ab insecticidal protein in combination with a Cry 1Fa insecticidal protein to delay or prevent development of resistance by the insect(s).

An embodiment of a method for detecting low frequency occurrence of one or more HIV sequence variants associated with drug resistance is described that comprises the steps of: generating cDNA species from each RNA molecule in an HIV sample population; amplifying at least one first amplicon from the cDNA species, wherein each first amplicon comprises a plurality of amplified copies and is amplified with a pair of nucleic acid primers that define a locus of the first amplicon; clonally amplifying the amplified copies of the first amplicons to produce a plurality of second amplicons wherein a plurality of the second amplicons comprise an immobilized population of substantially identical copies from one of the amplified copies of first amplicons; determining a nucleic acid sequence composition of the substantially identical copies from at least 100 of the immobilized populations in parallel on a single substrate; and detecting one or more sequence variants that occur at a frequency of 5% or less in the nucleic acid sequence composition of the at least 100 immobilized populations; and correlating the detected sequence variants with variation associated with HIV tropism.

The tetracycline class of antibiotics has played a major role in the treatment of infectious diseases for the past 50 years. However, the increased use of the tetracyclines in human and veterinary medicine has led to resistance among many organisms previously susceptible to tetracycline antibiotics. The modular synthesis of tetracyclines and tetracycline analogs described provides an efficient and enantioselective route to a variety of tetracycline analogs and polycyclines previously inaccessible via earlier tetracycline syntheses and semi-synthetic methods. These analogs may be used as anti-microbial agents or anti-proliferative agents in the treatment of diseases of humans or other animals.

The invention belongs to the technical field of the farm chemical, and in particular relates to a plant insecticidal protective agent and an application thereof. The invention provides the plant insecticidal protective agent which is recomposed by food suppressing hydrazine and organic phosphorus (I), pyrethroids insecticides (ii), insect growth regulation agent (III), Macrolides (IV), heterocyclic insecticidal component (V), carbamates (VI) or Nereistoxin pesticide and the application thereof. The recomposed insecticidal composite remarkably improves the insecticidal effect, at least has four acting ways at the same time such as touch out, stomach toxicity, internal absorption and antifeedant so as to adequately protect the plant, favors the delay of the production of the drug resistanceof the insects through different acting ways, and enlarges the pest control spectrum. The composite can be widely applicable to the control of different chewing mouthparts, piercing-sucking mouthparts, lapping mouthparts and rasping mouthparts pests.

The invention discloses a special physiological regulator for maternal animals, and a preparation method thereof, and belongs to the field of feed additive. A technical scheme of the physiological regulator comprises: carrying out crushing and screening for dandelion, leonurus, licorice root and angelica; adding soybean meal and/or wheat bran; adding water to carry out immersing; adjusting pH value of the resulting mixture; carrying out a sterilization; inoculating probiotics to carry out a fermentation after cooling, followed by stoving after completing the fermentation to obtain the physiological regulator. With the prepared physiological regulator for the maternal animals, immunity and disease resistance of the maternal animals are raised, incidence rate of genital system infection is effectively reduced, feed inversion rate is increased, mammogenesis is improved, milk yield is raised, incidence rate of mammitis is reduced, and milk quality is improved. The physiological regulator has characteristics of quick onset, stable and reliable effect, no toxic and side-effect, no pollution, no residue, and no drug resistance generating, and meets requirements of safe feed additive and assurance of foods of animal origin. In addition, the physiological regulator for the maternal animals can be adopted as a veterinary drug through raising using dosage of the physiological regulator, and provides a substantial curative effect for prevention and cure of the mammitis.

The invention relates to plant endogenous bacillus velezensis which is capable of preventing and treating plant diseases and insects and has functions of growth promotion and drought resistance, and application thereof. The preservation number of the Bacillus velezensis E6 provided by the invention in the China General Microbiological Culture Collection Center is CGMCC No. 9665. The strain is separated from a plant root, is rapid to grow, can be rapidly and greatly colonized at plant roots and has ecological niche advantages; the strain E6 not only has an efficient prevention and treatment function on plant soil-borne fungal diseases and is capable of promoting growth of plant root systems and improving drought resistance of plants, but also is capable of damaging formation of pathogenic bacterium bacterial biofilms, is free of drug resistance of bacteria, has relatively good advantages when being compared with other fungicides, is simple in nutrition requirement and low in productioncost, and has good application prospects.

This disclosure provides tyrosine kinase protein and nucleic acid variants, particularly FGFR2 variants, which are linked to drug resistance. The disclosure further provides methods of diagnosis and theranosis, and development of new therapeutic agents using these molecules and fragments thereof, and kits for employing these methods and compositions.

The invention relates to a laying hen feed prepared from bran, salt, corn grit, fish meal, ground oyster shells, rice bran, bean dregs and a traditional Chinese medicine feed additive, wherein the traditional Chinese medicine feed additive comprises the following components: honeysuckle, common cnidium fruit, corydalis tuber, small fennel, motherwort, self-heal, houttuynia, baikal skullcap root, dyers woad leaf, dangshen, astragalus root, angelica sinensis, medical stone, eclipta, dodder, barbat skullcap and weeping forsythia. The feed additive provided by the invention has both medicinal and nutritional actions, can prevent diseases and can also improve the production performance; the feed additive can directly sterilize and inhibit bacteria, adjusts the immunity function of of the organism, has non-specific antibacterial action, and greatly improves the survival rate of chicken; and the feed additive eliminates chemical damage to the chicken, can effectively improve the production performance of animals, simultaneously has small toxic side effects and no drug resistance, can effectively improve the egg quality, achieves the requirements of purely natural green food, and can acquire good economic benefit.

Intact bacterially derived minicells containing functional nucleic acids or plasmids encoding functional nucleic acids can reduce, in targeted mammalian cells, drug resistance, apoptosis resistance, and neoplasticity, respectively. Methodology that employs minicells to deliver functional nucleic acids, targeting the transcripts of proteins that contribute to drug resistance or apoptosis resistance, inter alia, can be combined with chemotherapy to increase the effectiveness of the chemotherapy.

The present invention provides methods and compositions for identifying cancer cells that are either sensitive or resistant to a particular anti-cancer therapy. Accordingly, the present invention allows for more accurate diagnosis, prognosis, and monitoring of a subject's condition. Furthermore, the ability to assess a subject's resistance or sensitivity to a particular treatment regimen will permit more informed treatment decisions to be made prior to beginning therapy. The present invention also overcomes deficiencies in the prior art concerning the treatment of cancers by providing methods and compositions for treating cancer and improving the effectiveness of other cancer therapies.

The Chinese medicine for treating cardiac and cerebral vascular diseases is prepared with American ginseng, astragalus root, angelica, leatherleaf milletia, saffron and other 23 kinds of Chinese medicinal materials. It has the functions of dilating blood vessel, antagonizing coagulation, eliminating thrombus, improving blood circulation, etc. It is used in treating coronary heart disease, angina, myocardial ischemia and other cardiac and cerebral vascular diseases. It has the advantages of short treating period, fast acting, no resistance, no toxic side effect and high effective rate.

The invention relates to a pig feed additive capable of replacing antibiotics, and a preparation method and an application thereof. Components and weight part ratio thereof in a formula are as follows: 1-5 parts of medicated leaven, 1-5 parts of angelica sinensis, 1-5 parts of radix astragali, 1-5 parts of hawthorn, 1-5 parts of radix codonopsis, 10-20 parts of citric acid, 10-20 parts of lactic acid, 20-30 parts of coated phosphoric acid, 5-10 parts of a micro-ecological preparation, 5-10 parts of yeast cell wall polysaccharide, 1-5 parts of a composite plant essential oil and 5-10 parts of a carrier. The pig feed additive carries out combination collocation of traditional Chinese herbs and functional polysaccharide, the micro-ecological preparation, acidifying agents, plant essential oil and the like, so that synergic effect of the traditional Chinese herbs and other components are maximized; the pig feed additive can replace the antibiotics completely; problems of drug resistance, drug residues, overcommitment and the like of the antibiotics which trouble animal husbandry for a long time is reduced; and at the same time, the pig feed additive plays effects of improving culture environment, reducing feed safety hidden dangers, increasing culture benefits and the like.

The invention relates to a pesticide composition containing indoxacarb, mainly used in crops disinfestation, which comprises emulsifiable concentrate, wettable powder and suspension agent. The active ingredients of the pesticide composition are indoxacarb and active ingredient B which is any one or composition of more than one with any proportion of Methylamino abamectin benzoate, hexaflumuron, nitenpyram, avermectin, fipronil, chlorfenapyr, acetamiprid, profenofos, phoxim, high-efficiency cypermethrin, cyhalothrin, triazophos and chlorpyrifos. The pesticide composition has the advantages of enlarging the application range, having strong contact toxicity and stomach poisoning action, having low mammalian toxicity and better compatibility of environment, being effective to the pest of every growth period, increasing the effect of every component of the composition significantly, reducing the production cost and agricultural cost of the pesticide, having wide insecticidal spectrum and high efficiency, delaying the occurrence of the drug resistance of the pests, prolonging the service life of the pesticide agent, enabling the high yield and high quality of the crops, having strong activity, long persistent period and stable drug efficacy to the pests.

The present invention provides methods and compositions for said inhibition of drug resistance. In one embodiment, said invention provides methods and compositions for said inhibition of antibiotic resistance. The invention generally involves said administration of achaogens, agents that inhibit said mutational process, to inhibit said evolution of drug resistance. Also, described herein are compositions that are suitable for use as achaogens.

The invention discloses an sgRNA directing sequence for specific targeting of a human ABCG2 gene and an application thereof, and belongs to the field of gene engineering application. The sgRNA directing sequence has a nucleotide sequence of GCTGCAAGGAAAGATCCAAG. According to the sgRNA directing sequence, two single-stranded oligo sequences are designed and synthesized and then are annealed to form a double chain, and then the double chain is connected with a Cas9 vector; with use of the Cas9 vector, sgRNA and a CRISPR system are introduced into target cells, a Cas9 protein can find out a DNA sequence matched with the Cas9 protein under guidance of the sgRNA, shearing is performed, and the ABCG2 gene is knocked out. According to the sgRNA directing sequence provided by the invention, the ABCG2 gene is knocked out or edited by the CRISPR-Cas9 system, then the expression of the ABCG2 is inhibited or eliminated, and the problem of multi-drug resistance generated in treatment of tumor can be effectively solved.

The invention relates to pentadeuteropyridine compounds represented by the following formula (I) and pharmaceutically acceptable salts, stereoisomers, prodrugs and solvates thereof, and a preparation method, pharmaceutical compositions and uses thereof. The compounds can generate an inhibitory effect on variation forms of epidermal growth factor receptor (EGFR) protein kinase, thereby effectively inhibiting the growth of a variety of tumor cells; the compounds can be used for preparation of antitumor drugs, are used for treatment or prevention of a plenty of different cancers, and moreover, can overcome the drug resistance induced by conventional drugs gefitinib, erlotinib and other first-generation EGFR inhibitors. More specifically, the compounds can be used for preparation of drugs for treatment or prevention of diseases, obstacles, disorders or illness conditions mediated by certain variation-form epidermal growth factor receptors (such as L858R activated mutants, Exon19 deletion activated mutants, and T790M resistance mutants).

The invention provides a recombinant vector for eliminating the activity of a kanamycin drug resistance gene, and aims at eliminating drug resistance germs in organisms and solving the problem of kanamycin drug resistance of the germs. The recombinant vector for eliminating the activity of the kanamycin drug resistance gene is characterized by comprising a pCas9 vector subjected to chloramphenicol resistance elimination and a gRNA nucleotide sequence KR58 or KR208 aiming at a kanamycin resistance gene kan; the concrete nucleotide sequence of the KR58 is GCCGCGAT TAAATTCCAACA, and the concrete nucleotide sequence of the KR208 is CAATGATG TTACAGATGAGA. A building method of the recombinant vector mainly comprises the steps of carrying intergenic region nucleic acids by a novel gene editing tool CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9 system; removing a chloramphenicol resistance gene on the recombinant vector; transforming the gene into vaccine vector bacteria such as attenuated salmonella typhimurium; performing co-culture on the recombinant bacteria and the kanamycin drug resistance gene so that the recombinant vector in the recombinant bacterium cell enters the kanamycin drug resistance bacteria in an engaging mode. The activity of the kanamycin resistance gene kan is effectively inhibited, so that the original drug resistance bacterium cannot grow on a kanamycin culture medium.

The invention discloses mosquito repellent liquid and a preparation method thereof. The mosquito repellent liquid comprises raw material liquid made of raw materials including, by weight, 5-20 parts of agastache rugosus, 5-20 parts of clove, 5-20 parts of moxa leaves, 5-15 parts of radix angelicae, 5-15 parts of honeysuckle, 5-15 parts of mint, 5-10 parts of perilla leaves and 5-10 parts of wild pepper. The mosquito repellent liquid is made of various raw materials, is reasonable and scientific in formula and has remarkable mosquito repellent functions, drug resistance is not easily generated as various drugs are combined, each ingredient is a natural plant ingredient or a Chinese medicine ingredient, the raw materials are easily obtained, the mosquito repellent liquid is green, non-poisonous and environment-friendly, the environment is not polluted when the mosquito repellent liquid is used, and the mosquito repellent liquid is high in safety, simple and convenient to manufacture and use, low in cost and suitable for the general public.