Anti-disease, growth promotion and drought-resistant functional plant endogenous bacillus velezensis and application thereof

A technology of bacillus and microbial strains, applied in forestry and horticultural crop protection, and agricultural fields, can solve problems such as differences, achieve rapid growth, short fermentation cycle, easy large-scale production and commercial application

Active Publication Date: 2018-05-08
河北伊诺生化有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on the fermentation process of Bacillus velezensis strains at home and abroad. There are only two domestic reports (Wang Zhenhai et al., 2011; Zhen Xinwu and Qiao Junjian, 2013). The Bacillus velezensis strains used in these two studies were screened from pig manure. Yes, the suitable fermentation temperature required is 37°C, but the suitable growth temperature of Bacillus E6 from the root of marigold is 28-32°C, and other nutritional requirements also vary due to different sources

Method used

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  • Anti-disease, growth promotion and drought-resistant functional plant endogenous bacillus velezensis and application thereof
  • Anti-disease, growth promotion and drought-resistant functional plant endogenous bacillus velezensis and application thereof
  • Anti-disease, growth promotion and drought-resistant functional plant endogenous bacillus velezensis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1, the isolation and identification of Bacillus velezensis E6

[0055] (1) Isolation of Bacillus E6 strain

[0056] Bacillus velezensis (Bacillus velezensis) E6 of the present invention is obtained from the marigold root, after diluting and coating the tissue grinding liquid, separating and obtaining, and the separating method is:

[0057] (1) Isolation of Bacillus Velez: The roots of plants such as marigolds were collected at the experimental base of the Academy of Agricultural Sciences in Langfang City, Hebei Province. Weigh 1g root, wash it with 1% NaClO sterilized water, put it into a sterile mortar and grind it, use a pipette gun to draw 1mL grinding solution from the mortar, add it to another test tube filled with 9mL sterile water, and mix well , and so on to make 10 -3 、10 -4 、10 -5 Solutions of different dilutions. Take 100 μL of the diluted solution and spread it on a TSA medium plate, spread it on three culture dishes for each gradient, and incub...

Embodiment 2

[0074] Preparation of Bacillus velezensis E6 bacterial agent

[0075] 1. The bacterial lawn of Bacillus velezensis E6CGMCC No.9665 obtained in Example 1 was transplanted into nutrient broth (NB), and shaken at 28-30° C. for 20 hours to obtain seed liquid.

[0076] Among them, the formula of nutrient broth (NB) is: glucose 10g, peptone 10g, beef extract 3g, yeast extract 1g, distilled water 1000mL, pH adjusted to 7.0-7.2, autoclaved at 121°C for 30 minutes.

[0077] 2. Inoculate the seed liquid obtained in step 1 into a large amount of culture and fermentation culture liquid according to the ratio of 1% (volume ratio), the culture temperature is 30-32 ° C, the rotation speed is 150-180 rpm, the initial pH is 7.0-7.2, and the culture time is 28 hours . Fermentation liquid is obtained after the fermentation is completed, and the number of live bacteria of Bacillus velezensis E6CGMCC No.9665 in the fermentation liquid can reach 12 billion / mL.

[0078] 3, the bacterium slime obta...

Embodiment 3

[0079] Embodiment 3: the disease prevention effect of bacillus E6 bacterial agent

[0080] Control Effects on Cucumber Fusarium Wilt : Fusarium was cultured with Armstrong culture medium first, counted under the microscope after 3 to 4 days, and the bacterial solution was diluted to 5×10 4 cfu / mL for use; dilute the E6 bacterial agent to 1×10 7cfu / mL. Mix the Fusarium spore suspension and the E6 bacterial agent in equal volumes, then add the sterilized seeds into the mixture and soak for 30 minutes. There were 10 repetitions per treatment, and 3 cucumber seeds were sown in each repetition, and the treatment without fungal agent was used as the control. 3 weeks after sowing, the growth and disease of cucumbers were investigated.

[0081] The components and proportioning of the Armstrong culture solution are: glucose 20.0g, KCl 1.6g, MgSO 4 .7H 2 O0.4g, Ca(NO 3 ) 2 5.9g, KH 2 PO 4 1.1g, FeSO 4 0.2μg / mL, ZnSO 4 0.2μg / mL, MnSO 4 0.2μg / mL, distilled water 1000mL, aut...

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Abstract

The invention relates to plant endogenous bacillus velezensis which is capable of preventing and treating plant diseases and insects and has functions of growth promotion and drought resistance, and application thereof. The preservation number of the Bacillus velezensis E6 provided by the invention in the China General Microbiological Culture Collection Center is CGMCC No. 9665. The strain is separated from a plant root, is rapid to grow, can be rapidly and greatly colonized at plant roots and has ecological niche advantages; the strain E6 not only has an efficient prevention and treatment function on plant soil-borne fungal diseases and is capable of promoting growth of plant root systems and improving drought resistance of plants, but also is capable of damaging formation of pathogenic bacterium bacterial biofilms, is free of drug resistance of bacteria, has relatively good advantages when being compared with other fungicides, is simple in nutrition requirement and low in productioncost, and has good application prospects.

Description

technical field [0001] The invention belongs to the technical field of protection of agricultural, forestry and horticultural crops, and in particular relates to a Bacillus Velez strain which prevents and cures plant soil-borne diseases and has the function of promoting growth and resisting drought and its application. technical background [0002] Bacillus is a widely distributed Gram-positive rod-shaped aerobic bacteria. Because it can produce endophytic spores, it has strong resistance to heat, radiation, and chemical drugs, and is easy to be processed into preparations. And be widely used. The commercial bacillus currently used for plant disease control is mainly Bacillus subtilis preparations. For example, Bacillus subtilis GB03, MBI600 and QST713 developed in the United States, Bacillus subtilis RB-14 and NB-22 developed by Tokyo Institute of Technology in Japan, Bacillus subtilis RC8, Bacillus subtilis RC9 and Bacillus subtilis RC11 developed in Argentina, Bacillus ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/07
CPCA01N63/10C12N1/20C12N1/205C12R2001/07
Inventor 郭荣君王军强李世东荆玉玲高毓晗孙瑞梁
Owner 河北伊诺生化有限公司
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