This invention relates to the field of biomedical materials, specifically to an antibacterial hydrogel, its preparation method, and its applications. The method involves first preparing thiolated
tannic acid and
copper-doped
Prussian blue nanozyme (CuPB). Methacrylated
gelatin is mixed with the thiolated
tannic acid, followed by the sequential addition of CuPB, ε-
polylysine,
trehalose,
horseradish peroxidase, and a
photoinitiator. The mixture is stirred until homogeneous to obtain a photosensitive
prepolymer solution, which is then photocured under visible light to obtain the antibacterial hydrogel. The antibacterial hydrogel prepared by this invention responds to changes in H2O2 concentration at the
wound site: during infection, CuPB and HRP
cascade catalyze the generation of
reactive oxygen species, synergistically with ε-
polylysine and
copper ions to achieve multi-mechanism, highly efficient
antibacterial action; simultaneously, it triggers oxidative crosslinking of the thiolated
tannic acid, enhancing its mechanical properties and accompanied by
color changes, thus aiding in monitoring the infection status; possessing excellent
biocompatibility, antibacterial properties, and mechanical properties, it can be used in wound dressings, biosensors, and other fields.