The invention relates to an underfill adhesive and a preparation method thereof. The underfill adhesive comprises the following ingredients by weight percent: 30 to 70 percent of filler, 10 to 50 percent of epoxy resin, 2 to 20 percent of curing agent, 0.1 to 0.5 percent of catalyst, 1 to 15 percent of flexibilizer, 1 to 25 percent of diluting agent, 0.1 to 3 percent of dispersing agent, 0.05 to 1 percent of defoaming agent, 0.1 to 1 percent of coupling agent and 0.1 to 0.5 percent of pigment; the filler comprises a first component and a second component, wherein the first component is spherical silicon dioxide, and the second component is selected from one of spherical nano aluminum oxide, spherical nano boron nitride, spherical nano aluminum nitride, spherical nano silicon carbide, spherical nano silicon nitride and spherical nano diamond powder. The filler comprises the first component and the second component, and a heat conduction channel can be formed in the underfill adhesive, so that the heat conduction coefficient can be increased; and the underfill adhesive has high heat conductivity and can be widely applied to high-density systematic encapsulation.