The invention relates to a quasi-one-dimensional metal oxide nano-material biosensor and a method for manufacturing the same. The sensor comprises a silicon chip, a silicon dioxide oxidation layer grown on the silicon chip, a grid electrode, a source electrode, a drain electrode and a microfluid channel; and a quasi-one-dimensional metal oxide semiconductor nano-material is connected with the source electrode and the drain electrode to form a conduction channel. A process for the quasi-one-dimensional metal oxide nano-material biosensor comprises the following steps: firstly, synthesizing thequasi-one-dimensional metal oxide semiconductor nano-material; secondly, adopting a micro-nanometer photolithography standard process and a top-down method to manufacture the quasi-one-dimensional metal oxide semiconductor nano-material and a field effect transistor in array; thirdly, using polydimethylsiloxane to manufacture the microfluid channel; finally, performing surface modification on thequasi-one-dimensional metal oxide semiconductor nano-material, modifying a joining unilayer combined with a target molecule through a self-assembling method, and connecting biological molecules on the surface of the nano-material through joining molecules so as to detect a symbolic molecule of a disease. The quasi-one-dimensional metal oxide nano-material biosensor has the characteristics of rapid response, high sensitivity, strong selectivity, no labeled molecule and the like.