231 results about "Syphilis" patented technology

The invention relates to a special multi-channel micro-flow controller used in syphilis diagnosis and using low-priced conducting material, which pertains to the field of analysis and test. One of theaims of progress of relevant diagnosis and treatment technology is to quickly diagnose syphilis disease with low price. The invention provides a diagnosis device which can realize the aim. The key points thereof are as follows: the controller is internally provided with four branch pipelines which are connected with each other in parallel; four working electrodes are respectively arranged in thefour branch pipelines; the working electrode consists of a conductive electrode and a gold colloid sensitive film which is embedded with a syphilis specific antibody and attached on the conductive electrode; the gold colloid sensitive films of the four working electrodes are respectively embedded with four different syphilis specific antibody substances; the four antibody substances are respectively syphilis specific antibodies of TP0684, TP0453, TP0821 and TP0319; and the working electrodes take the shape of fibers, and the bodies of working electrodes is made of material of thermal decomposition and conductive polymer. The chip of the proposal has low cost and a plurality of channels.

The invention provides a Chinese herbal preparation for treating posirasis, which adopts 58 raw medicinal materials such as astragalus root, suberect spatholobus stem, Job stears, glabrous greenbrierrhizome, spreading hedyotis herb, barbed skullcap herb, motherwort herb and the like to manufacture oral agent and abrasive cleaner. The manufacturing method of the invention is as follows: 1. manufacturing by using the above raw material medicines into water-decocted liquid according to the conventional method; 2. manufacturing by using the water-decocted liquid which is manufactured in step 1 into oral liquid according to the conventional method; 3. drying, grinding and sieving the water-decocted liquid which is manufactured in step 1, and taking the throughs as powder; and 4. manufacturinginto powder tablets, granular formulation, medicinal granules or capsules by utilizing step 3 according to the conventional method. The powder and the abrasive cleaner can be manufactured by the following steps: 1. grinding the above raw material medicines; 2. sieving; and 3. taking the throughs, disinfecting and sterilizing to obtain the powder. The invention has high cure rate, low recurrence rate, can treat the primary and the secondary aspects at the same time, is safe and reliable, and can strengthen the autoimmunity. The invention mainly treats various types of posirasis, can treat neurodermatitis, comedo, acne and eczema concurrently, and has effects on lupus erythematosus, syphilis, lepra and skin cancer.

The invention discloses a kit of chemiluminescent immunological analysis measurement of a Treponema pallidum antibody, and preparation method thereof, which belongs to the technical field of immunological analysis medical diagnosis. The kit comprises (1) a carrier coated with a treponema pallidum specific recombination protein antigen; (2) treponema pallidum antibody negative and positive reference substances; (3) an enzyme labeled treponema pallidum specific recombination protein antigen; and (4) an enzyme acted chemiluminescent primer. Furthermore, the preparation method of the kit based on the invention comprises the following steps of (1) preparing TP antibody negative and positive reference substances; (2) labeling the TP specific recombination protein antigen with an enzyme; (3) coating with the carrier; (4) sub-packaging; and (5) assembling. The kit of the detection of the Treponema pallidum antibody has the advantages of easy and simple operation, easy popularization, high sensitivity, strong specificity, good repeatability, safety, non toxicity, and no pollution.

The invention relates to a syphilis serological screening or diagnosis method. The purpose is that the method has the characteristics of low price, high speed, sensitivity and specificity. The technical scheme provided by the invention is that: a preparation method of rTpN15-17-47-ELISA for detecting syphilis serum antibodies comprises the following steps in turn: constructing an artificial fusion gene tpN15-17-47 of tpN15, tpN17 and tpN47 and a prokaryotic expression system E.coliBL21DE3Pet42a-tpN15-17-47 by gene technology, purifying the recombinant expression product rTpN15-17-47 as a coating antigen, establishing rTpN15-17-47-ELISA for detecting syphilis serum antibodies. The fusion gene tpN15-17-47 has a nucleotide sequence and an amino acid sequence as shown in sequence table 5.

The invention discloses a centrifugal chip for quickly detecting hepatitis B, hepatitis C and syphilis in blood and a detection method. The chip is characterized in that main bodies are in disc shapes and at least four microfluidic unit modules are uniformly distributed on each main body; each microfluidic unit module comprises a first lotion pool (1), a second lotion pool (2), a reaction pool (3), an enzyme solution pool (5), a waste liquid pool (8), two detection liquid pools (6) and a terminating liquid pool (7). The detection method comprises the following steps: injecting a detection liquid into each unit module in advance during detection; then adding a sample; and centrifugalizing in the levels. The chip and detection method disclosed by the invention can be used for quickly improving the detection efficiency.

The invention provides a reagent strip for joint detection of a syphilis specific IgM and IgG antibodies and a preparation method thereof, relating to a reagent for detection of a syphilis specific antibody. The invention provides the reagent strip for joint detection of the syphilis specific IgM and IgG antibodies and the preparation method thereof. The reagent strip is provided with a vector plate, a loading pad, a colloidal gold pad, a nitrocellulose membrane, a syphilis specific IgG antibody detection line, a syphilis specific IgM antibody detection line, a contrast line and an absorption pad. The preparation method comprises the following steps: preparing sample application of nitrocellulose membranes for recombinant syphilis antigens TPN17 and TPN47, preparing colloidal gold, labeling syphilis specific antigen TPN17 and TPN47 with colloidal gold and preparing the reagent strip for joint detection of the syphilis specific IgM and IgG antibodies.

An instant Chinese medicine for treating dermatopathy, such as dermatitis, etch, eczema, tinea, psoriasis, etc, is prepared from 8 Chinese-medicinal materials including houttuynia, wild chrysanthemumflower, dandelion herb, China root, etc through pulverizing, sieving by 30 meshes, infrared sterilizing and baking. Its advantages include high effect on sterilizing, relieving inflammation and resisting virus, and no by-effect.

A medicine for treating the intractable tinea, including psoriasis and syphilis, is prepared from 17 raw materials including VB1, VB2, VB6, chlorpheniramine, furazolidone, dexamethasone acetate, etc.

The invention relates to a special multichannel syphilis diagnosis device combined with quasi-one-dimensional specific antigen modified electrodes. The device is a micro-fluidic chip, belonging to the field of analysis test. One of the targets of the relevant diagnosis and treatment technologies is to quickly diagnose syphilis patients with low cost. The invention provides a diagnosis device aiming to achieve the target. The key points of the proposal are that the device, namely the micro-fluidic chip, comprises channels of parallel structure inside, the parallel structure comprises four branch channels mutually connected in parallel, four catenulate working electrodes in total are respectively arranged in the four branch channels, the catenulate working electrode is composed of a conductive electrode and a gold size sensing membrane that attaches onto the conductive electrode and is embedded with the syphilis specific antibody, and the respective gold size sensing membranes on the superficial coats of the four catenulate working electrodes are respectively embedded with four different syphilis specific antibody substances which are respectively syphilis specific antibodies TP0684, TP0453, TP0821 and TP0319.

The invention relates to a syphilis-diagnosis special multichannel micro-fluidic chip based on an electrode modification technology, belonging to the field of analytical testing. The quick and cheap diagnosis of syphilitic patients is one of the improved objectives of a relative diagnosis and treatment technology. The invention provides a micro-fluidic chip which points to the objective. The scheme is characterized in that pipelines with parallel structure are arranged in the chip, the parallel structure comprises four branched pipelines which are connected in parallel, four magnetic bead string working electrodes are respectively assembled in the four branched pipelines, each magnetic bead string working electrode comprises a magnetic bead string electrode and a gold size sensitive film, the gold size sensitive film is attached to the magnetic bead string electrode and is embedded with syphilitic specific antigens, and the gold size sensitive films arranged on the surfaces of the four magnetic bead string working electrodes are respectively embedded with four different syphilitic specific antigen materials of syphilitic specific antigens TP0684, TP 0453, TP 0821 and TP 0391. The micro-fluidic chip is beneficial to improving the syphilitic diagnostic efficiency.

The invention discloses a sanitary napkin, toilet paper or care wings containing taxus chinensis and preparing methods thereof. The sanitary napkin, toilet paper or care wings comprises an outer layer of permeable nonwoven cloth layer, a middle layer of fiber layer and an inner layer of netted curtain cast film layer; the fiber layer contains medicine powder made of Taxus chinensis, or the fibre layer is soaked by taxus chinensis leaching liquor, or the netted curtain cast film layer is soaked by Taxus chinensis juice, wherein the content of Taxus chinensis is 10% to 100% of the total content of medicine; furthermore, one or more following components is selected: fructus cnidii, which has the functions of drying dampness, dispelling wind, and curing trichomonas vaginitis; cortex phellodendri, which has the functions of clearing heat, drying dampness, purging fire, removing toxicity, antibiosis, and anti-trichomonad; sophora flavescens, which has the functions of clearing heat, drying dampness, killing worms, relieving the itching, antibiosis, and anti-tumor; smilax glabra, which has the functions of removing toxicity and dampness, anti-tumor, and curing syphilis; dictamni cortex, which has the functions of clearing heat, drying dampness, dispelling wind, removing toxicity, and anti-tumor; and rhizoma corydalis, which has the functions of promoting blood circulation to remove blood stasis, and boosting Qi to relieve pains.

The invention provides a reagent strip for joint detection of a syphilis specific IgG antibody and a specific total antibody and a preparation method thereof, relating to a reagent for joint detection of a syphilis specific IgG antibody and a specific total antibody. The reagent strip is provided with two reagent strips, wherein one is provided with a vector plate, a sample adding pad, a colloidal gold pad, a nitrocellulose membrane, a syphilis specific IgG antibody detection line, a control line and an absorbent pad; and the other one is provided with a vector plate, a sample adding pad, a colloidal gold pad, a nitrocellulose membrane, a specific total antibody detection line, a control line and an absorbent pad. The preparation method comprises the following steps: preparing recombinant syphilis antigens TPN17 and TPN47, applying samples of nitrocellulose membranes, preparing colloidal gold, labeling TPN17 and TPN47 with colloidal gold and preparing immunochromatography detection strips. The reagent strip can be used for detecting the syphilis specific IgG antibody and the specific total antibody in the specimens of whole blood, blood serum, blood plasma, cerebrospinal fluid and the like. During detection, the specimen amount is small, special instruments are not needed and the results are directly interpreted by the naked eyes. The reagent strip is simple, convenient, fast, accurate and reliable and has strong specificity and high sensitivity.

A protein chip for testing the infectious diseases from the taken blood in blood bank, its preparing process and its testing method are disclosed. The antibodies or antigens and positive and negativereferences of heapatitis B, hepatitis C, AIDS and syphilis are densely arrayed on solid carrier, to which the protein is linked via chemical bond. Said protein is arrayed in a cavity of multi-sample microarray biochip. The reaction results for more antibodies or antigens of different blood samples can be obtained by one-pass reaction. Its advantages are high sensitivity and high specificity.

The invention provides a reagent strip for joint detection of a syphilis specific IgM antibody and a specific total antibody and a preparation method thereof, relating to a reagent for joint detection of a syphilis specific IgM antibody and a specific total antibody. The reagent strip is provided with two reagent strips, wherein the two reagent strips are both provided with vector plates, sample adding pads, colloidal gold pads, nitrocellulose membranes, control lines and absorbent pads and respectively provided with a syphilis specific IgM antibody detection line and a specific total antibody detection line. The preparation method comprises the following steps: preparing recombinant syphilis antigens TPN17 and TPN47, applying samples of nitrocellulose membranes, preparing colloidal gold,labeling TPN17 and TPN47 with colloidal gold and preparing immunochromatography detection strips. The reagent strip can be used for detecting the syphilis specific IgM antibody and the specific totalantibody in the specimens of whole blood, blood serum, blood plasma, cerebrospinal fluid and the like. During detection, the specimen amount is minimal, special instruments are not needed and the results are directly interpreted by the naked eyes. The reagent strip is simple, convenient, fast, accurate and reliable and has strong specificity, high sensitivity, low cost and wide application range.

The invention relates to an immune chromatography kit used for simultaneously realizing screening of three or four of four major infectious diseases (AIDS, hepatitis B, hepatitis C and syphilis) by a single experiment. The invention has the characteristics as follows: the particle diameter of a colloidal gold is increased to 80 to 90nm from the typical 20 to 40nm, so that the sensitivity of the colloidal gold is improved; two enveloping modes are available, for instance, all test items are underlined on the same NC membrane with one separate from another, and assembled on the same test strip finally, or, for instance, all test items are separated and underlined on different NC membranes, and are arranged and assembled separately; and then, the envelope antigens / antibodies of all test items are mixed together and underlined, thereby resulting in only one test line. The invention has the advantages that the invention can be applied to screening prior to physical examination, blood donation, blood transfusion, surgery and the like, so that the test time can be saved, the efficiency can be improved, the HIV P24 antigens can be tested as early as possible, and the window period of HIV test can be shortened.

This method can test if a patient has been infected by syphilis or hepatitis B virus with a drop of blood, which combines two test measures for testing diseases of syphilis antibody and hepatitis B surface antigen on a same test paper, utilizes immune chromatographic technology and automatic micro-spraying equipment to fix the pured syphilis recombination antigen and anti-HBS single-clone antibody on a qualified nitrocellulose filter then to mix them labelled with colloidal gold in certain proportion to prepare a golden combined pad attached by proper sample process pads to form test paper slip, which quickly tests the syphilis antibody or hepatitis B surface antigen in a person blood, serum or plasma by double-antigen sandwich and double antibody sandwich methods.

An antigen composition and method for the detection of antibodies to Treponema pallidum and the diagnosis of syphilis are described. The antigen composition contains synthetic cardiolipin and synthetic lecithin. The antigen composition may additionally contain cholesterol and an alcohol. The antigen composition is useful as an immunoreagent in immunoassays for the detection of antibodies associated with T. pallidum infection. The methods are sensitive and specific for T. pallidum infection.

The invention discloses a medicament for treating syphilis, and a preparation method thereof. The medicament is prepared by the following medicaments according to a certain weight percentage: smilax glabra rhizome, honeysuckle flower, dittany bark, cicada skin, dragon's bone, black-tail snake, hornet nest, Chinese honeylocust spine, and the like. The medicament has the functions of removing syphilis, dispelling dampness, clearing heat, removing toxicity and supporting healthy energy, and is fast in action, good in curative effect and high in cure rate.

The invention discloses a nano iodine-chitosan cervical disease treating membrane agent and a preparation method of the membrane. The treating membrane is prepared by adding plasticizer and adsorbent to chitosan, povidone iodine, dialkyl quaternary ammonium salt and chlorhexidine acetate used as main effective ingredients. The treating membrane disclosed by the invention has killing activity to multiple pathogenic microorganisms, has no irrigation to mucosa, can relieve itching, arrest bleeding and promote the growth of epidermic tissues, can be used for treating colpitis, trichomonas vaginalis, cervicitis, cervical erosion and other gynecological diseases, has better repair action on vaginal and cervical injury, and can effectively prevent the propagation of venereal diseases such as gonorrhoea, syphilis, AIDS (acquired immunodeficiency syndrome) and the like.

A Chinese medicine for treating dermatopathy and venereal disease, including eczema, tinea, vulvitis, pointed condyloma, gonorrhea, syphilis etc is prepared from Chinese-medicinal materials including pearl, calomel, realgar, etc through pulverizing and mixing with vasuline. Its advantages are high curative effect, quickly taking its effect, no by-effect and no recurrence.

The invention provides a western blot kit for cardiolipin and specific IgM antibodies of syphilis and preparation thereof, relating to a kit. The kit is provided with a carrier plate, a cellulose nitrate membrane, a detection line of the specific IgM antibodies and cardiolipin IgM antibodies of the syphilis and a control line, wherein the detection line and the control line are arranged on the cellulose nitrate membrane in sequence; specific recombinant antigens and cardiolipin antigens of the syphilis are coated at the detection line of the specific IgM antibodies of the syphilis, and human IgM antibodies are coated at the control line; and horse radish peroxidase is marked on anti-human mu-chain antibodies. The preparation comprises the following steps: preparing the specific recombinant antigens of the syphilis, then preparing the cardiolipin antigens, carrying out sample application on the cellulose nitrate membrane, and preparing mu-chain monoclonal antibodies of anti-human IgM specific segments; and marking the horse radish peroxidase on the anti-human IgM specific segments mu-chain monoclonal antibodies, and then preparing the western blot kit. The kit provided by the invention can be used for detection of the specific IgM antibodies and cardiolipin antigens of the syphilis in specimens such as whole blood, blood serum, blood plasma, cerebrospinal fluid and the like.

The invention relates to a kit, in particular to a western blot kit for syphilis specific total antibodies and anticardiolipin antibodies and a preparation method of the kit. The kit is provided with a carrier plate, a nitrocellulose membrane, detection lines for syphilis specific total antibodies and anticardiolipin antibodies and a comparison line, wherein the detection lines and the comparison line are arranged on the nitrocellulose membrane; syphilis specific recombinant antigens and anticardiolipin antigens are coated at the detection lines for the total antibodies and anticardiolipin antibodies; anti-human Ig (immunoglobulin G) antibodies are coated at the comparison line; and horse radish peroxidase is labeled with the anti-human Ig antibodies. The method comprises the following steps: preparing the syphilis specific recombinant antigens; preparing the anticardiolipin antigens; applying samples of the nitrocellulose membrane; preparing anti-human total specific segment Ig chain monoclonal antibodies; labeling the horse radish peroxidase with the anti-human Ig monoclonal antibodies; and preparing the western blot kit. The western blot kit can be used for detecting the syphilis specific total antibodies and the anticardiolipin antibodies in the specimens of whole blood, serum, blood plasma, cerebrospinal fluid and the like. The invention has the advantages of small required specimen quantity, strong specificity, high sensitivity and is simple and fast in detection, and accurate and reliable in detection, and special instruments are not needed.

The invention relates to a treponema pallidum IgG antibody biotin avidin enzyme-linked immune detection kit and a preparation method thereof, which relates to the syphilis detection. The kit comprises a concentrated washing liquid bottle, a negative and positive control bottle, a biotin mark antihuman IgG specific fragment gamma chain monoclonal antibody bottle, a horseradish peroxidase mark avidin bottle, a TMB coloured solution bottle A, a coloured solution bottle B, a reaction stopping solution bottle, a micropore plate coated with recombinant antigen. The treponema pallidum has infectious standard strain Nichol strain and treponema pallidum non-infectious standard strain Reiter strain, by using a proteomics method, the treponema pallidum proteomics can be separated through dimensional electrophoresis, protein with strong immunogenicity can be identified by different patient or infectious animal serum, and then an antigen marker can be searched. The antigen marker is used for syphilis confirmation test, so that diagnosis sensitivity and specificity can be increased, a window stage is shortened. The treponema pallidum antibody can be sensitively detected and enables quantitative measurement, and has the advantages of cheap price, simple operation and accurate result.

The invention discloses a group of protein marker for simultaneously detecting the specificity of hepatitis A, hepatitis B, hepatitis C, Aids, syphilis and tuberculosis in blood serum, a kit for detecting the group of specific proteins and application thereof. The protein marker for detection the specificity in the invention comprises seven proteins with different molecular weights in a serum proteome map: A: 5232.13, B: 8784.25, C: 2017.5, D: 4309.19, E 5489.97, F: 8076.62 and G: 2155.92. A detection method for the specific proteins comprises the steps of: processing a serum sample to be detected by using a lysis solution; incubating and capturing serum proteins by using a nano magnetic bead with carboxyl as a genin; and eluting the nano magnetic bead, and detecting seven specific proteins in eluted ingredients by using a protein reader. The kit and the detection method in the invention have the advantages of rapidness, sensitivity, specificity, flexibility, simplicity for operation and the like.

The invention discloses an LAMP primer combination for detecting three ophthalmic infection spirochetes and application. The primer combination is composed of eighteen single-stranded DNA molecules shown from the sequence 1 to the sequence 18. The invention further provides application of the primer combination. The invention furthermore provides a method of using the primer combination for identifying whether a spirochete to be detected is treponema pallidum, or borrelia burgdorferi or leptospira, a method for identifying whether the spirochete to be detected is treponema pallidum, or borrelia burgdorferi or leptospira and a method for identifying whether a sample to be detected is infected by treponema pallidum and / or borrelia burgdorferi and / or leptospira. Treponema pallidum, borrelia burgdorferi and leptospira can be fast and accurately detected by using the method.

The invention relates to a multi-channel device for syphilis diagnosis with a strongly hydrophobic substrate of a microfluidic chip, which belongs to the field of analysis and testing. It is a technical goal to use cheap and easy-to-process polydimethylsiloxane (PDMS) to make the substrate of the microfluidic chip for syphilis diagnosis. The gist of this case is that the substrate is selected from PDMS with an original ecological surface, and the hinged fixture with a micro-ultrasonic transducer is manually fastened and positioned at the position adjacent to the sample liquid flow terminal of the microfluidic chip, so as to The ultrasonic wave induces the sample liquid flow to flow from the sample injection end of the chip along the pipe of the chip to the terminal. The pipe of the chip is filled with micron-sized silicon dioxide particle fillers. The function of the filler includes supporting the inner wall of the pipe, And, a collection of hydrophilic micro-channels is used to compensate the originally hydrophobic channels, and the inner wall of the channel is squeezed and covered to reduce the chance of biomacromolecules in contact with the inner wall.

The invention relates to a traditional Chinese medicine composition for treating skin disease and venereal disease, and belongs to the field of traditional Chinese medicines. The traditional Chinese medicine composition comprises an oral administration prescription. The oral administration prescription is prepared from, by weight, 2-4 parts of coptidis rhizome, 2-4 parts of radix scutellariae, 2-4 parts of cortex phellodendri, 2-4 parts of radix sophorae flavescentis, 2-4 parts of radix sophorae flavescentis, 1-3 parts of rheum officinale. The traditional Chinese medicine composition can effectively treat skin disease and venereal disease, particularly malnutrition sores, psoriasis, pruritus of vagina, syphilis and the like.

A method of capturing multiple virus germ biological label at biological chip absorption surface and carrying out analysis to captured biological label can be used to detect multiple biological label group such as hepatitis B, hepatitis C, Aide and syphilis in blood in order to identify normal person or person with abovesaid disease.

The invention discloses an immunosensing carrier and a kit for syphilis antibody detection, and a preparation method of the carrier. The immunosensing carrier for syphilis antibody detection comprises a porous membrane; the porous membrane is connected with a specific anti-silk membrane antibody; the specific anti-silk membrane antibody is connected with a cross-linked antibody; and the cross-linked antibody is connected with the TP specific antibody and the recombinant TP antigen. The immunosensing carrier and the kit for syphilis antibody detection provided by the invention can realize rapid and accurate detection of the TP antibody.