19 results about "Syphilis" patented technology

The invention relates to a syphilis non-specific antibody detection result automatic interpretation method and system, and belongs to the technical field of biomedical detection. The method comprises the following steps: acquiring a sample image after agglutination reaction through shooting equipment, and transmitting the sample image to an automatic interpretation system through a wireless network; the system preprocesses the image, extracts morphological features, distribution features and texture features of agglutination particles by using a pre-trained AI model, and fuses metadata such as patient age and detection time to generate a fusion feature vector; performing similarity matching with a historical sample database based on the feature vector, and automatically interpreting a result through a weighting rule engine; and the interpretation result is output in a differentiated visual form, and the subjectivity of manual interpretation is eliminated and the problem of inconsistent results is solved through a unified image preprocessing and feature extraction method.

The invention discloses a novel leuconostoc screening method and an antibacterial mechanism of leuconostoc on leaf mold of zanthoxylum bungeanum, and relates to the technical field of pathogenic bacteria experiment and analysis. The novel leuconostoc screening method comprises the following steps that S1, materials are prepared, specifically, rotten Chinese prickly ash tree plants are selected, and tested strains in the Chinese prickly ash tree plants comprise plant pathogens of alternaria alternata, fusarium solani, fusarium layered, fusarium oxysporum, fusarium syphilis and colletotrichum pini, and are stored in a refrigerator of a laboratory at the temperature of 4 DEG C for standby application; in the invention, the leuconostoc also has an efficient inhibition effect on field crop pathogenic fungus alternaria alternata, and the research expands the biocontrol spectrum of lactic acid bacteria. Meanwhile, the invention discloses a molecular mechanism that the Leuconostoc suionicum HJ-L synergistically regulates and controls the synthesis of antibacterial substances through lipid metabolism, an ABC transport system and an autophagy pathway, and a theoretical basis and a practical basis are provided for the application of the strain in green prevention and control of the leaf mold of Chinese prickly ash.

The invention provides a composite quality control product of an AIDS antibody, a syphilis antibody and a hepatitis C antibody as well as a preparation method and application of the composite quality control product. The composite quality control product comprises an AIDS (Acquired Immune Deficiency Syndrome) antibody, a syphilis antibody, a hepatitis C antibody and the following components: a matrix solution, 1.5 to 3 g / L of Pluronic F68, 0.05 to 0.15% v / v of ProClin 300, 0.1 to 1 g / L of sodium surfactin, 1 to 10 g / L of bovine serum albumin, 1 g / L of alanine, 2 to 5 g / L of trehalose, 0.5 to 1.0 g / L of EDTA (Ethylene Diamine Tetraacetic Acid) and 2 to 5 mmol / L of benzamidine. The matrix liquid comprises a buffer solution and 20-30% v / v human serum. According to the invention, the aggregation and inactivation risk of the antibody in the freezing and thawing process is effectively reduced, the antibody can be repeatedly frozen and thawed for more than three times, the degradation of the antibody at high temperature can be effectively prevented, and the reliability of the quality control product in the transportation and clinical use process is ensured.

The invention discloses an early and late latent syphilis prediction model based on a treponema pallidum nonspecific IgM antibody, and relates to the technical field of biology. The objective of the invention is to solve the problem of inaccurate latent syphilis staging caused by dependence on subjective recall and single index in the prior art. The prediction model is a hume diagram model and comprises three indexes, namely a treponema pallidum non-specific IgM antibody, a treponema pallidum specific antibody and a treponema pallidum non-specific antibody. The method provides a basis for timely and accurately performing medical intervention on early and late latent syphilis patients, and has an important clinical application value.

The invention belongs to the technical field of biological detection, and particularly relates to a primer group and a kit for detecting new plum brown spot and application of the primer group and the kit, and base sequences of the primer group are shown as SEQ ID NO.1-SEQ ID NO.5. Based on the primer group, the loop-mediated isothermal amplification technology is used for rapid detection of the alternaria nepalensis, and the alternaria nepalensis can be accurately detected from a complex pathogenic bacterium environment in diseased plant tissues. The rapid detection system disclosed by the invention is good in specificity and high in sensitivity, and the detection limit of the DNA of the alternaria nepalensis is 0.246 fg / mu L. By utilizing the rapid detection system provided by the invention, the alternaria nepalensis can be rapidly, simply, accurately and sensitively detected from a field sample within 45 minutes, and compared with a traditional symptom recognition method and a common molecular detection method, the rapid detection system is simpler and more efficient.

The invention belongs to but not limited to the technical field of human and animal health diagnostics, and discloses a binding molecule for acute infection diagnosis adapted to a point-of-care (POC) scene, an acute infection diagnosis system and a kit. The binding molecule is an antibody, a nano antibody or an aptamer (preferably not combined with IgM) which is specifically combined with a J chain of dIgA, steric hindrance and conformation rearrangement are avoided by combining the J chain, and the flexibility of a compound is kept so as to efficiently combine with an antigen; the diagnostic system and the kit are adapted to POC scenarios (such as lateral flow test strips). The problems that POC deployment is difficult, RAT sensitivity is low and dIgA detection efficiency of CSC is low in existing NAT are solved, and the kit has the advantages of being low in cost, high in specificity and good in stability, can be used for rapid diagnosis of multiple acute infections such as measles and syphilis, fills up related technical gaps and has high commercial value.

The utility model discloses a bearing device for a special card for a non-treponema pallidum antigen serum test, which belongs to the technical field of serum tests.The bearing device for the special card for the non-treponema pallidum antigen serum test comprises a supporting and placing mechanism, and the supporting and placing mechanism comprises a supporting seat; the protection mechanism comprises a limiting anti-overflow assembly, the limiting anti-overflow assembly comprises a fixed anti-overflow plate and a fixed supporting frame, the fixed anti-overflow plate and the fixed supporting frame are connected to the two ends of the top of the supporting base in a limiting mode, and movable anti-overflow plates are slidably connected to the two sides of the fixed anti-overflow plate and the two sides of the fixed supporting frame in a limiting mode; an experiment card is placed in a frame defined by the fixed spill-proof plate, the fixed supporting frame and the movable spill-proof plate, auxiliary grooves are symmetrically formed in the two sides of the supporting base, fingers, wearing gloves, of medical workers can be conveniently placed in the auxiliary grooves, meanwhile, the bearing device can be easily pinched up, direct contact with the card is avoided, reactant contamination is prevented, and operation safety is improved.

The invention relates to an antigen construct for rapid immune detection, in particular to an antigen molecule for realizing dimerization or trimerization through foldon or a variant thereof. The antigen construct can enhance the conformation stability and improve the antibody binding efficiency, so that the sensitivity and shelf life stability in colloidal gold, fluorescent microspheres, ELISA (enzyme-linked immuno sorbent assay) and other in-vitro diagnosis methods in immunochromatography are remarkably improved. The construct is suitable for antibody detection of rabies, influenza, coronavirus, Brucella, tuberculosis and the like in zoonosis and HIV, HBV, HCV, syphilis and the like in human infectious diseases, and can be widely applied to clinical screening, animal quarantine, family self-test and public health emergency monitoring.

The utility model provides a sample adding and smearing device for a syphilis non-specific antibody detection test, relates to the technical field of medical instruments, and aims to solve the problems that the device is inconvenient to smear and completely spread samples on different reaction cards at the same time, and when the sample size is large, a long time difference exists between early-stage sample adding and later-stage sample adding, so that the time difference is large. The device comprises a suction tube and a sample adding tube, wherein the suction tube is arranged on the suction tube; a group of fixing blocks are slidably mounted at the outer end of the suction tube, a group of pistons are slidably mounted in the suction tube, and the fixing blocks at the outer end of the suction tube are inserted into limiting grooves in the top of the movable frame; according to the reaction card smearing device, samples on different reaction cards can be conveniently smeared at the same time and completely spread, the use efficiency of the device is effectively improved, the smearing plate can be conveniently and automatically fixed at the horizontal position, and the use effect of the device is further improved.

The application provides a complex quality control product of HIV antibody, syphilis antibody and hepatitis C antibody and a preparation method and application thereof. The complex quality control product comprises HIV antibody, syphilis antibody and hepatitis C antibody and the following components: a matrix liquid, 1.5-3 g / L Pluronic F68, 0.05-0.15 % v / v ProClin 300, 0.1-1 g / L sodium mycobacterial lipopeptide, 1-10 g / L bovine serum albumin, 1 g / L alanine, 2-5 g / L trehalose, 0.5-1.0 g / L EDTA and 2-5 mmol / L benzamidine; the matrix liquid comprises a buffer and 20-30 % v / v human serum. The application effectively reduces the aggregation and inactivation risk of the antibodies in the freeze-thaw process, can be repeatedly freeze-thawed more than three times, can effectively prevent the degradation of the antibodies under high temperature, and ensures the reliability of the quality control product in the transportation and clinical use process.

The invention provides a traditional Chinese medicine preparation formula for treating sexual infectious diseases. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 5-15 parts of coptis chinensis, 5-15 parts of scutellaria baicalensis, 5-15 parts of golden cypress, 5-15 parts of cape jasmine, 10-20 parts of honeysuckle, 10-20 parts of dandelion, 5-15 parts of chrysanthemum, 5-15 parts of philippine violet herb, 5-15 parts of gynura bicolor, 10-20 parts of houttuynia cordata, 5-15 parts of hairyvein agrimony, 5-15 parts of patrinia, 5-15 parts of fructus forsythiae, 10-30 parts of astragalus, 5-10 parts of divaricate saposhnikovia root, 5-15 parts of reed rhizome, 5-15 parts of salvia miltiorrhiza, 5-15 parts of poria cocos and 5-10 parts of curcuma zedoary. 5-15 parts of lucid ganoderma, 5-15 parts of bighead atractylodes rhizome and 5-10 parts of tendril-leaved fritillary bulb; according to the traditional Chinese medicine composition, large-dose heat-clearing and detoxifying medicines such as coptis chinensis, scutellaria baicalensis, golden cypress, honeysuckle and dandelion are used as monarch medicines, fructus forsythiae, houttuynia cordata and patrinia herb are used as auxiliary medicines, and the traditional Chinese medicine composition has broad-spectrum inhibition and killing effects on pathogens of various sexually transmitted diseases such as gonococcus, treponema pallidum, HSV, HPV, HIV and parasites, and can effectively control infection symptoms.

The utility model provides a syphilis non-specific antibody detection device, and belongs to the technical field of medical instruments. The problem of misreading of a critical sample due to manual operation and reading in the existing syphilis non-specific antibody detection is solved. The syphilis non-specific antibody detection device comprises a transparent sample cup, an optical detection system and a stirring structure used for uniformly mixing a sample and a reagent, the optical detection system comprises a transmitting end arranged on one side of the sample cup and a receiving end arranged on the other side of the sample cup, and the transmitting end is used for transmitting light to the receiving end; the receiving end is used for receiving and processing the light penetrating through the sample cup. A detection process curve is drawn through an optical detection system, the reaction value of the whole stage can be tested, and a positive detection result value can be accurately given.

Embodiments relate to compositions, methods, and systems designed to screen and detect antibodies for pathogen detection. Embodiments particularly relate to systems that utilize multilayered plasmonic nanoparticles for enhanced detection sensitivity. Embodiments further relate to systems designed to simultaneously detect treponemal and non-treponemal antibodies for syphilis diagnoses.

The invention discloses a test strip for rapidly detecting syphilis and / or HIV (human immunodeficiency virus) antibodies. The test strip comprises a back plate, and a water absorption pad, an NC (nitrocellulose) membrane, a conjugate pad and a sample pad which are laid on the back plate in sequence, the combination pad is coated with a syphilis recombinant antigen covalently coupled with red latex microspheres, an HIV recombinant antigen covalently coupled with red latex microspheres, staphylococcus aureus A protein covalently coupled with red latex microspheres and a chicken IgY antibody covalently coupled with red latex microspheres; the NC membrane is provided with a detection line T1, a detection line T2 and a quality control line C; the detection line T1 is coated with a syphilis recombinant antigen; the detection line T2 is coated with an HIV (Human Immunodeficiency Virus) recombinant antigen; the quality control line C is coated with an anti-chicken IgY antibody; according to the detection test strip, a double-antigen sandwich method is adopted, and the characteristic that SPA is strongly combined with the Fc segment of the antibody is supplemented, so that the antibody can be captured in an extremely low antibody concentration, the sensitivity is remarkably improved, and particularly, the detection rate of urine and a window phase sample is improved.

This disclosure provides a device and a method for combining a lumbar puncture needle and an ultrasonic density detection circuitry to provide an inexpensive and accurate lumbar puncture locator device. The disclosed device senses different tissue densities with different frequency tones and colored light indicators before needle insertion in the patient's lumbar vertebrae region. This invention allows a doctor, a nurse practitioner or a physician assistant to safely do a lumbar puncture in an office environment without the scheduling of the use of the radiology department. This device will allow a timelier diagnosis of infections (such as Lyme disease, bacterial and viral meningitis, herpes, West Nile virus, syphilis), multiple sclerosis, Alzheimer's dementia, tumors, idiopathic intracranial hypertension, subarachnoid hemorrhage, and others.

Provided are primers and probes for single-tube multiplex fluorescent PCR detection of Treponema pallidum, herpes simplex virus 1 and herpes simplex virus 2, a fluorescent PCR kit, and the use thereof. The primers comprise specific sequences for Treponema pallidum, herpes simplex virus 1 and herpes simplex virus 2, respectively, which sequences have no cross-reaction. The specific sequences of the primers and probes are as shown in SEQ ID NO:1 to SEQ ID NO:9. A plurality of sexually transmitted pathogens such as Treponema pallidum, herpes simplex virus 1, and herpes simplex virus 2 can be detected simultaneously.

The invention discloses a multi-copy nucleic acid target fragment for detecting treponema pallidum, the nucleic acid target fragment comprises a nucleic acid fragment as shown in SEQ ID NO: 1, and application of the multi-copy nucleic acid target fragment in nucleic acid detection of various nucleic acid amplification platforms. The invention further relates to a primer designed based on the target fragment, a detection kit and application of the primer and the detection kit in syphilis diagnosis and curative effect evaluation in all stages. The multi-copy nucleic acid target fragment TMF7 provided by the invention can be detected in a sample with treponema pallidum greater than or equal to 6 copies / ml, has very high sensitivity, and is remarkably superior to Tpp47 and polA (greater than or equal to 1000 copies / ml); through clinical verification, the detection rate of TMF7 in blood plasma, saliva and cerebrospinal fluid of syphilis patients in all stages is remarkably higher than that of existing Tpp47 and polA targets; in addition, the method is suitable for detecting treponema pallidum in all samples, and industrialization and large-scale popularization are easy.

System and method for remote self-collection of a blood sample for assessment at a lab to detect presence of specific sexually transmitted infections (STIs). An at-home collection kit may include an ability to detect chlamydia (CT), gonorrhea (GC), bacterial vaginosis (BV), candida vaginitis (CV) and Trichomonas vaginalis (TV), HSV-2, syphilis, HIV, HBsAg, an HCV. The screening of samples for the presence of antigens and / or antibodies to infectious agents is a primary laboratory tool for the diagnosis of infections of the above viruses and bacterium. Distributing a specimen collection kit containing dried blood spot (DBS) cards can eliminate the hurdles of visiting clinics or hospitals and reduce stigma. DBS samples collected on filter paper, such as GE Whatman™ 903 protein saver cards, offer several advantages over on-site phlebotomist collection. The DBS cards and collection devices are especially useful for individuals who live in remote areas where healthcare resources may be limited.

The invention relates to the technical field of biomedicine, in particular to a monoclonal antibody pair for resisting treponema pallidum TP protein and application of the monoclonal antibody pair. The antibody pair comprises two monoclonal antibodies; the monoclonal antibody comprises a heavy chain variable region and a light chain variable region; one monoclonal antibody in the monoclonal antibody pair is named as a 5C12 antibody, and the other monoclonal antibody is named as a 1E8 antibody. The invention also discloses a nucleic acid molecule for coding the monoclonal antibody and a hybridoma cell strain for secreting the monoclonal antibody. In addition, the invention also discloses a kit for detecting treponema pallidum TP protein, which comprises the monoclonal antibody pair, the 5C12 antibody is used as a labeled antibody, and the 1E8 antibody is used as a capture antibody. The anti-treponema pallidum TP protein monoclonal antibody pair provided by the invention can realize high-specificity and high-sensitivity detection on the syphilis.