The invention discloses a
Treponema pallidum
antibody detection kit and a preparation method thereof. The
Treponema pallidum
antibody detection kit is composed of the following components: TP
antigen-
coating magnetic microspheres, a negative reference substance, a positive reference substance, a TP
antigen marker bound to lanthanides, an analytical buffer, a cleaning solution, an enhancement solution and an RFID card. Based on combination of the magnetic microspheres and a time-resolved
immunoassay method, the drawbacks of long physical
adsorption reaction time and slow detection result of anenzyme
label plate are overcome, and the reaction time is greatly shortened; and besides, the advantages of high accuracy, high sensitivity, strong specificity, wide
linear range, and stable and convenient detection of a time-resolved detection technology can also be realized. The magnetic microspheres are coated with corresponding antigens or antibodies, due to the stereoscopic characteristic ofthe magnetic microspheres, the contact surface area of an immune response is greatly increased, the detection time is greatly shortened, and a result can be obtained through detection in 30 minutes.