36 results about "Treponema pallidum antibody" patented technology

The invention aims to provide a reagent for assaying an antiphospholipid antibody which is excellent in long-term storage stability and capable of diagnosing syphilis infection or the like and a reagent for assaying an anti-Treponema pallidum antibody which is capable of diagnosing syphilis infection with high accuracy by preventing the occurrence of serum interference. The invention is directed to the reagent for assaying an antiphospholipid antibody to be used for diagnosing syphilis infection comprising an insoluble carrier carrying an antiphospholipid antigen and a copolymer with a segment derived from 2-methacryloyloxyethyl phosphorylcholine and a segment derived from a hydrophilic monomer.

The invention belongs to the technical field of immunoassay and diagnosis and relates to a method for magnetic antibody immunoassay chemiluminescence detection of treponema pallidum antibodies. The method comprises the following steps of: using GoldMag particles as carriers, coating one or more antigens of specific recombinant proteins of treponema pallidum antibodies, TP15, TP17, TP47 and TP44.5, then adding a sample to be detected and enzyme labeled antigens, and finally adding luminescence substrates for luminescence detection. The GoldMag particle surface has a larger coupling capacity to the antigen and has characteristics of high specificity and no pollution of the enzyme linked immunoassay as well as high sensitivity of chemiluminescence, and therefore, the method has the advantages of high detection sensitivity, good specificity, wide linear range, no radioactive pollution and the like.

The invention relates to a treponema pallidum (TP) antibody test kit based on the flow microspheric carrier technology and a preparation method and detection method thereof, belonging to the technical field of immunoassay medical diagnosis. The preparation method comprises the following steps: using TP recombinant antigen to coat heavy polymer microsphere, using bovine serum albumin to seal empty binding site, preparing specific TP probe-heavy polymer microsphere; performing coculture with a sample to be tested to capture TP antibody, washing and centrifuging to remove the unbound TP antibody, then adding fluorescently-labeled anti-human IgG or IgM antibody; and using a flow cytometry to detect the fluorescence intensity of the microsphere, and performing qualitative or quantitative analysis to the tested antibody. The method has the advantages of high sensitivity and specificity and good stability and can be used to perform microanalysis or multivalent analysis to the sample.

The invention discloses a kit for detecting a treponema pallidum antibody as well as a detection method and an application thereof, and belongs to the technical field of in vitro diagnosis and detection. The kit consists of the following components: (1) a marker system: including a treponema pallidum recombinant antigen 1 in direct or indirect connection to a marking tracer, or staphylococcus aureus A protein in direct or indirect connection to the marking tracker; (2) a bridged compound system: including a treponema pallidum recombinant antigen 2 in direct or indirect connection to a first bridged compound; and (3) a magnetic microsphere system: comprising a magnetic microsphere in direct or indirect connection to a second bridged compound, wherein the first bridged compound is combined with the second bridged compound. The kit and the detection method disclosed by the invention can promote the full implementation of conjugation reaction between antigen and antibody; and detection sensitivity can be improved.

The related fast detection agent for syphilis spirochete antibody comprises a fast detection paper prepared by cellulose nitrate covered by pure syphilis spirochete monoclonal antibody and dried gold-mark monoclonal antibody. Wherein, with colloid gold immune chromatography technology and double antigen sandwich way, detecting syphilis spirochete and its fragment in humor, secretion and culture. This invention has more clinic meaning, high specificity, well sensitivity, and fit to wide spread.

The invention relates to a treponema pallidum IgG antibody biotin avidin enzyme-linked immune detection kit and a preparation method thereof, which relates to the syphilis detection. The kit comprises a concentrated washing liquid bottle, a negative and positive control bottle, a biotin mark antihuman IgG specific fragment gamma chain monoclonal antibody bottle, a horseradish peroxidase mark avidin bottle, a TMB coloured solution bottle A, a coloured solution bottle B, a reaction stopping solution bottle, a micropore plate coated with recombinant antigen. The treponema pallidum has infectious standard strain Nichol strain and treponema pallidum non-infectious standard strain Reiter strain, by using a proteomics method, the treponema pallidum proteomics can be separated through dimensional electrophoresis, protein with strong immunogenicity can be identified by different patient or infectious animal serum, and then an antigen marker can be searched. The antigen marker is used for syphilis confirmation test, so that diagnosis sensitivity and specificity can be increased, a window stage is shortened. The treponema pallidum antibody can be sensitively detected and enables quantitative measurement, and has the advantages of cheap price, simple operation and accurate result.

It is an object of the present invention to provide a reagent for assaying anti-phospholipid antibodies, which is excellent in long-term storage stability and enables an accurate diagnosis of syphilitic infection, and a reagent for assaying anti-Treponema pallidum antibodies, which enables an accurate diagnosis of syphilitic infection by preventing an occurrence of serum interference.

The present invention is a reagent for assaying anti-phospholipid antibodies used for diagnosing syphilitic infection, which contains an insoluble carrier supporting an anti-phospholipid antigen thereon and a copolymer having a segment derived from 2-methacryloyloxyethyl phosphorylcholine and having a segment derived from a hydrophilic monomer.

The invention relates to a recombinant chimeric antigen of treponema pallidum. The recombinant chimeric antigen is formed by connecting an amino acid sequence as shown in SEQ ID NO.12, a first flexible linker, an amino acid sequence as shown in SEQ ID NO.9, a second flexible linker and an amino acid sequence as shown in SEQ ID NO.13 in series. The recombinant chimeric antigen can be used for serological detection of treponema pallidum antibody, and has improved dissolving expression capability, activity and thermal stability. The invention also relates to a method for preparing the recombinantchimeric antigen.

The invention discloses a treponema pallidum antibody detection kit. The kit comprises a magnetic separation reagent, a biotin antigen, an enzyme-labeled reagent comprising a detection antigen and a detection antibody coupled alkaline phosphatase, a negative control, a positive control and a calibration solution. The biotin antigen and detection antigen are recombinant antigens. The detection antibodies comprise a mouse monoclonal antibody and a mouse second antibody. The calibration solution contains an antibody to be detected. The invention discloses a detection method of the kit. Through combination of an indirect method and a double antigen sandwiching method, double antigen sandwiching method specificity is guaranteed and the problem that according to the double antigen sandwiching method, two solid phase antigens or two tracer-labeled antigens are bonded to the same antibody so that detection is unsuccessful and false positive or false negative detection result is avoided. The combined indirect method and double antigen sandwiching method produce effects better than those of the single indirect method or double antigen sandwiching method.

The invention belongs to the field of biological detection technologies and relates to a treponema pallidum antibody detection card, a kit and a detection method. The treponema pallidum antibody detection card comprises test paper and a plastic shell; the test paper comprises a bottom plate; a sample pad, a bonding pad, nitrocellulose membrane and absorbent paper are sequentially arranged on the bottom plate from the left to right of the bottom plate; the nitrocellulose membrane is provided with a detection zone and a control zone from the left to right of the nitrocellulose membrane; a glassfibre membrane is soaked by a sample pad treatment liquid and dried, so that the sample pad can be obtained; a mixed immunogold solution is sprayed onto the glass fibre membrane, so that the bonding pad can be obtained; a treponema pallidum recombinant antigen diluted by a coating solution is sprayed onto the nitrocellulose membrane, so that the detection zone can be formed; and an anti-chicken IgY antibody diluted by the coating solution is sprayed onto the nitrocellulose membrane, so that the control zone can be formed. The detection card of the invention has the advantages of convenience inapplication and accurate results, and provides a basis for the accurate treatment of syphilis in the next step, and is suitable for being popularized and applied on a large scale.

The invention relates to the technical field of in vitro diagnosis, in particular to a treponema pallidum antibody detection kit. The kit comprises treponema pallidum antigen coupling magnetic particles, a treponema pallidum antigen enzyme conjugate and a chemiluminiscence substrate, the treponema pallidum antigen enzyme conjugate is a buffer solution containing a treponema pallidum antigen markedby a biological enzyme, urokinase and calf serum. According to the invention, magnetic particles are used as a solid-phase carrier of the antibody, and a chemiluminescence immunoassay technology is utilized, so that single-person, random, rapid and automatic detection can be realized on a full-automatic chemiluminescence analyzer. Tests prove that adding 0.1-30U/mL of urokinase into the enzyme conjugate has no influence on indexes such as sensitivity, specificity, stability, precision and the like, but the clinical retest rate is greatly improved.

The invention discloses a kit for detecting a treponema pallidum antibody in urine, a preparation method and a use method. The kit comprises a detection test strip. The detection test strip comprisesa sample pad, a combination pad, a nitrocellulose membrane, absorbent paper and a PVC bottom plate, wherein the sample pad, the combination pad, the nitrocellulose membrane and the absorbent paper aresequentially adhered to the PVC bottom plate; the combination pad is coated with a recombinant protein A (SPA)-colloidal gold marker, and the nitrocellulose membrane is respectively coated with a detection line composed of TP recombinant antigen and a quality control line composed of rabbit anti-SPA. Besides, a urine sample is concentrated through a concentration medium, so that the content of the treponema pallidum antibody in urine is increased, and the sensitivity of the kit for detecting the treponema pallidum antibody in the urine is improved. The detection sensitivity can be improved by2 to 6 times. While the kit is used, the urine is used as a detection sample, and sampling operation can be conducted in asafe, simple, convenient and noninvasive manner. And the kit is high in sensitivity, strong in specificity, convenient to operate and suitable for detection under various conditions.

To provide a reagent for assaying anti-Treponema pallidum antibody which reagent contains a polypeptide antigen and which reagent provides high assay sensitivity and high specificity, and to provide an assay method employing the assay reagent.

The reagent for assaying anti-Treponema pallidum antibody, for use in an assay of anti-Treponema pallidum antibody on the basis of antigen-antibody reaction is characterized in that the reagent contains, as an antigen, a recombinant polypeptide containing at least domain C and domain D of Treponema pallidum 47 kDa antigen but containing no domain A1 of the 47 kDa antigen.