2 results about "Treponema" patented technology

The present application discloses an application of a biomarker in the preparation of a product for diagnosing patients with oral diseases and a kit for detecting oral diseases, wherein the oral diseases include at least one of periodontitis, gingivitis and caries, and the biomarkers include cytokines and / or oral microorganisms, wherein the cytokines include at least one of IFN-γ, IL-6, IL-10, IL-18 and IL-1β, and the oral microorganisms include at least one of Neisseria, Porphyromonas, Fusobacterium and Treponema. In the present application, auxiliary diagnosis of oral diseases can be achieved by detecting biomarkers, which is conducive to timely detection of the condition in the early stage of oral diseases or before the disease progresses for timely intervention.

The invention belongs to the technical field of biology, and discloses a method for determining a multivalent antigen sequence of an anti-periodontitis red complex, which comprises the following steps: firstly, screening out key virulence factor antigens (FimA-II, RgpA, Msp and BspA) from three core pathogenic bacteria of the red complex: porphyromonas gingivalis, treponema denticola and fusisterone; then, independently cloning each antigen gene to a pUC57 cloning vector, constructing a single antigen recombinant plasmid, and avoiding intergene interference by designing a specific enzyme cutting site; and finally, through plasmid transformation, positive clone screening and double enzyme digestion verification, the plasmid quality is ensured. The obtained single antigen plasmid can be flexibly combined and used, is beneficial to subsequent multivalent vaccine development, and has the advantages of wide antigen coverage, high expression reliability, flexible construction strategy, easiness in industrialization and the like.