96 results about "Immunologic Diagnosis" patented technology

The invention provides a hollow silicon magnetic microsphere, as well as the preparation method and the application thereof. The hollow silicon magnetic microspheres have the preparation method that a polystyrene/silicon dioxide composite microsphere is used as a core; Fe3O4 coats the periphery of the core through the layer-by-layer self-assembly method; then, a PS/SiO2/Fe3o4 polymeric magnetic composite nanosphere is obtained, and polystyrene is removed through the high temperature sintering method, thereby forming a magnetic material with hollow structure; the inner layer of the hollow magnetic material is SiO2, and the stability is good, thereby the material is not easy to collapse at high temperature; furthermore, the specific surface area of the material can be improved, and the material is a monodisperse hollow magnetic microsphere material. The method has the advantages of low cost and good repetitiveness. The obtained product not only can be used as a magnetic targeting drug carrier material, but also can be used in the biological and the medical fields such as biosensors, immunologic diagnosis, drug delivery and tumor targeting therapy as well as DNA isolation, etc.

The invention discloses an epitope of rheumatoid arthritis and application thereof, belonging to the technical field of immunologic diagnosis. The epitope is a polypeptide, and the amino acid sequence is disclosed as SEQ ID NO:1 in the sequence table. ELISA detection and patient serum reaction condition indicate that the epitope polypeptide can be specifically combined with IgG in the patient serum, and does not react with the serum of a health person. The epitope polypeptide can be used for preparing medicines for diagnosing rheumatoid arthritis.

The invention provides an immunity diagnosis test kit for detecting II-type dengue virus antigen, which comprises a porous reaction plate covering monoclonal antibody DV2-M6, a sample treatment liquid, a monoclonal antibody DV2-M15 marked with a label, a positive contrast, a negative contrast, a concentration washing liquid, a develop liquid and a termination liquid, wherein the monoclonal antibodies DV2-M6 and DV2-M14 of the test kit can be specifically combined with NS1 protein of II-type dengue virus, without cross reaction with other three kinds of serotype dengue viruses NS1 and respectively combined with different antigen points of NS1, while the check sensitivity of NS1 protein of II-type dengue virus can reach 3ng/ml and the check sensitivity of culture supernatant of II-type dengue virus infection cell is 8 power of Pan-E dengue early elisa test kit, thereby improving the sensitivity of clinical serum sample check.

The invention belongs to the technical field of immunodiagnosis, and discloses a thyroxine (T4) enzymatic chemiluminescence quantitative determination kit. The kit disclosed by the invention consists of a T4 standard product, an antibody pre-coated reaction plate, a horseradish peroxidase marker, diluent, cleaning mixture, and chemiluminescence substrate liquid. The kit disclosed by the invention has the advantages of quickness, handiness, sensitiveness, cheapness and high repeatability, and is an important index for diagnosis and therapeutic evaluation of hyperthyroidism and hypothyroidism. The invention has high clinic use value. The invention also provides a method to prepare the kit.

The present invention relates to a colloidal gold semiquantitative immunologic diagnosis test paper preparing and assembly method. Said test paper utilizes immunity chromatograph principle capable of rapid visual inspection nano colloidal gold marker and sample combined colour, judging detective semiquantitative result through test zone with parallel reference zone colour contrast.

The invention provides a method for marking protein by a carboxyl microsphere, belonging to the field of preparation of immunologic diagnosis kits. According to invention, in a system for marking the protein by the carboxyl microsphere, according to the content of carboxyls on the surface of the microsphere, the molar ratio of cross-linking agents EDC to -COOH is set to be (0.8-2) to 1, and the molar ratio of EDC to NHS or Sulfo to NHS is (1-3) to 1, so that the optimal ratio of the number of the carboxyls on the surface of the microsphere to the number of activated carboxyls is achieved; meanwhile, the pH is regulated, so that the reaction system is rapidly converted from condition beneficial for activation into state beneficial for antibody connection, the step of centrifugalization or tangential flow filtering is saved, and finally, the purposes of simplifying marking steps, saving cost, and marking protein by the carboxyl microsphere efficiently are achieved. The method provided by the invention can be applied to preparation of a detection kit taking the latex microsphere or the magnetic microsphere as a carrier, and has good market application prospect and economic value.

The invention belongs to the technical field of immunologic diagnosis and particularly relates to a human immunodeficiency virus (HIV) antibody detection kit using a microparticle chemiluminiscence method and a preparation method of the kit. The kit is composed of magnetic microparticles for detecting an HIV antibody, a tracing conjugate for detecting the HIV antibody, a negative control, a I type positive control, a II type positive control and an analyzing buffering solution. The invention further discloses the preparation method of the detection kit, which adopts a particle chemiluminiscence immunoassay technology; compared with ELISA (Enzyme-Linked Immunosorbent Assay), the technology has higher sensitivity and specificity and is suitable for the clinical auxiliary diagnosis of HIV.

Disclosed are a hepatitis C virus antigen enzyme-linked immunoassay reagent box and a method for making the same. The invention obtains the cell strain of excretive anti HCV core antigen by analyzing the core antigen array of the hepatitis C virus different type and cloning the core antigen gene of HCV, purifying out the high activity monoclonal antibody with four core aa expression sites of HCV, wherein the Cab1 and Cabs are used as coating antibodies, the Cab3 and Cab4 are used as enzyme labeled antibodies; employing double antibodies sandwich technology to prepare HCV-cAg ELISA diagnosing reagent box.

An immunochromatographic test strip for fluorescence quantitative detection of INHB and a preparation method thereof. The invention belongs to the technical field of immunodiagnosis. The purpose of the invention is to address the deficiencies in the prior art. The invention adopts the following technical solutions: a fluorescent The immunochromatographic test strip for quantitative detection of INHB is composed of a sample pad, a marker pad, a coating film, and an absorbent paper sequentially lapped on a PVC bottom plate. The marker pad is sprayed with avidin; the coating of the quality control line has specific identification Rabbit anti-avidin antibody for avidin. The invention has the following advantages: the detection line and the quality control line adopt an independent reaction system without mutual interference and influence, and adopt the T/C value method for calibration, which ensures the accuracy of the test results. Fluorescence immunochromatography is adopted, and the detection method has high sensitivity, simple operation and low cost. It can detect inhibin B in blood samples with a concentration as low as 10 pg/mL. The detector used does not require professional operators, and the detection result can be obtained in 15 minutes.

The present invention belongs to immunological parasitic disease diagnosis technology. The reagent kit is prepared via dye labeling process and includes labeling antigen solution compounded with 2BLN disperse blue dye, soluble schistosoma japoncium egg antigen, 1% sodium azide and bow serum albumen blocking liquid; and test paper strip with nitrocellulose as chromatographic film detection lines of human sheep antigen IgG and contrast lines of soluble schistosoma japonium egg resisting rabbit antigen IgG on the chromatographic film. The serum to be tested is mixed with the labeling antigen solution and the mixture liquid is tested with the test paper strip.

The disclosed dual-sandwich enzyme-linked immunologic diagnosis agent box for hepatitis-B core antibody comprises: a micro-porous reaction plate composed by the hepatitis-B core antigen recombinant with purity more than 90% and 5*104~5*105u/mg titer, and the enzyme compound composed by the hepatitis-B core antigen recombinant marked by HRP. This invention has high specificity and sensitivity and convenient to qualitative or semi-qualitative detection.

The invention discloses an immunologic diagnosis kit for detecting dengue virus NS1 antigen and application thereof. The kit comprises a capturing antibody and a detecting body combined with a marker, and the kit is characterized in that the capturing antibody consists of a monoclonal antibody 2E8A5, a monoclonal antibody DV2M6 and a monoclonal antibody 4B14A1, the detecting antibody is a monoclonal antibody 3B1A15, and the marker can be biotin, horseradish peroxidase, alkaline phosphatase, colloidal gold or fluorescein. The kit can specifically detect NS1 proteins of four serotype dengue viruses and does not have cross reaction with other viruses of flavivirus system, such as flavivirus and encephalitis B virus, the sensitivity of the kit is at least four times higher than that of detection of foreign commodity kits, and the kit can efficiently reduce the omission factor of the clinical application.

The invention discloses a preparation method for a gold labeled immucochromatographic test strip jointly marked through colloidal gold and latex microsphere. The preparation method comprises the following steps that 1 chloroauric acid is reduced through trisodium citrate to prepare colloidal gold solution; 2 the colloidal gold solution is used for marking protein molecules; 3 50 nm red latex microsphere is prepared or bought directly; 4 the protein molecule is marked by the latex microsphere; 5 quality testing is conducted on the marking colloidal gold solution and latex marking solution which are obtained through the above steps; 6 a nitrocellulose membrane is pasted on a PVC plastic bottom plate; 7 a sample pad, a combining pad, a water-absorbing pad, handle paper and Max glue are assembled on the PVC plastic bottom plate; 8 quality testing is conducted on the prepared gold labeled test strip according to the quality standard of the product. The preparation method for the gold labeled immucochromatographic test strip jointly marked through the colloidal gold and the latex microsphere has the advantages that the detecting sensitivity and detecting range of the gold labeled immucochromatographic test strip are improved, and the application area and quantitative application of colloidal gold immunologic diagnosis are enlarged in this way.

The present invention relates to an antigenic B-cell multi-epitope peptide linked in tandem in the outer membrane protein(Omp) U gene of vibrio mimicus, a making method and an application thereof, which belong to the field of molecular immunology. The B-cell multi-epitope peptide linked in tandem can induce fish to make protective immunity response to vibrio mimicus infection. In the present invention, the amino acid sequence of the antigenic B-cell multi-epitope peptide linked in tandem in the OmpU is shown in SEQIDNO:10. The B-cell multi-epitope peptide linked in tandem in the OmpU of vibrio mimicus is made by a genetic engineering technique. The verification of immune blotting analysis, specific antibody detection and immune animal protective experiments shows that the peptide can elicit efficient and specific protective humoral immunity response to vibrio mimicus infection. The B-cell multi-epitope peptide linked in tandem can be used for the immune diagnosis and the immune prevention and treatment of aquatic animal ascitic diseases. The present invention has high social benefit and economic benefit.

The invention relates to NT-proBNP recombinant albumin. The NT-proBNP recombinant albumin is provided with the amino acid sequence chosen from a) or b): a). the amino acid sequence showed in SEQ ID NO: 1; b). the amino acid sequence with NT-proBNP recombinant albumin reactivity after one or more amino acids are deleted, replaced or inserted. The invention also relates to a nucleotide sequence for coding the NT-proBNP recombinant albumin and a preparation method of the NT-proBNP recombinant albumin. The NT-proBNP recombinant albumin is provided with the immunogenicity the same as the NT-proBNP albumin, and high purity and better stability, which can replace the e natural NT-proBNP polypeptide, is used as a NT-proBNP immunologic diagnosis reagent standard, and establishes basis for further researching and developing the NT-proBNP detection kit.

The invention relates to a test paper strip for detecting a P24 antigen and particularly relates to a preparation method of a carbon quantum dot test paper strip for detecting a P24 antigen. The preparation method comprises the following steps of: bonding a sample pad and a water absorption pad respectively at two ends of a bottom plate, preparing a P24 carbon quantum dot pad and a P24 nitrocellulose membrane, bonding the sample pad, the P24 carbon quantum dot pad, the P24 nitrocellulose membrane and the water absorption pad in a lapping way in sequence on the bottom plate to obtain a test paper plate, cutting the test paper plate into a plurality of test paper strips conforming to defined widths, and hermetically storing the test paper strips. According to the invention, a known specific antibody or antigen is fixed in a zone of the cellulose membrane at first, a sample to be detected is added on the sample pad, a carbon quantum dot marking reagent on the carbon quantum dot pad is dissolved, and a large amount of carbon quantum dots are gathered on a detection band, so that bright light is emitted and specific immunologic diagnosis is achieved. The method is high in sensitivity and low in toxicity or free from toxicity in a detection process.

The invention provides a mouse anti-MCR-1 protein hybridoma cell strain, a monoclonal antibody and application. Ig variable region genes are cloned through mouse hybridoma monoclonal antibody screening and an RT-PCR method, and a hybridoma cell strain capable of stably secreting a mouse anti-MCR-1 protein antibody and a variable region sequence of the hybridoma cell strain are obtained. Systematic evaluation shows that the mouse anti-MCR-1 protein antibody has better performance in all aspects, so that the mouse anti-MCR-1 protein antibody is suitable for being used as an immunodiagnostic reagent for in-vitro diagnosis of MCR-1 protein antigen, the titer reaches 1: 1280000 or above, and the mouse anti-MCR-1 protein antibody is used for developing related in-vitro diagnostic reagents.

The invention relates to a parathyroid hormone kit and a preparation method thereof, belongs to the technical field of immunologic diagnosis, and solves the technical problems that a parathyroid hormone method in the prior art is low in accuracy, short in shelf life, complicated in operation, unstable in test result and high in cost. The kit disclosed by the invention comprises a reagent R1, a reagent R2 and a reagent R3; the reagent R1 is a buffer solution I containing streptavidin magnetic particles; the reagent R2 is a parathyroid hormone monoclonal antibody solution labeled with a chemiluminescent marker diluted by the buffer solution I; and the reagent R3 is a coupled labeled parathyroid hormone monoclonal antibody solution diluted by the buffer solution I. The kit detects parathyroidhormone by adopting a chemiluminescence immunoassay, and has the advantages of high detection sensitivity, accurate quantitative detection, simple operation, no radioactive risk, short detection timeand capability of detecting antigen and antibody type target substances.

The invention relates to a novel mycobacterium tuberculosis specific fusion protein as well as preparation and application thereof and belongs to the technical field of tuberculosis medical immunologic diagnosis. The fusion protein is formed by sequentially connecting antigenic epitopes of two proteins Rv0057 and Rv1352, wherein the nucleotide sequence of the antigenic epitope of the protein Rv0057 is shown in a sequence 1 in a sequence table; and the nucleotide sequence of the antigenic epitope of the protein Rv1352 is shown in a sequence 2 in a sequence table. Compared with the current commercial antibody detection kit, the mycobacterium tuberculosis specific fusion protein disclosed by the invention has the advantages of high sensitivity, strong specificity and complementarity with other antigens in the aspect of tuberculosis serodiagnosis, and can be used for detecting specific anti-tuberculosis antibodies in body fluid samples such as blood serum and pleural effusion.

The invention relates to the technical field of immunologic diagnosis instruments, and particularly relates to a portable immune-chromatography analysis meter. The portable immune-chromatography analysis meter comprises a detection card fixing device, a large reflective mirror used for reflecting a detection area, a small reflective mirror used for reflecting a detection area image formed in the large reflective mirror, and an imaging sensor used for capturing the detection area image reflected by the small reflective mirror. According to the portable immune-chromatography analysis meter disclosed by the invention, effective image information of the overall detection area can be finally captured by the imaging sensor through two times of mirror reflection under a condition that the direct distance between the imaging sensor and the detection card is 2-6 cm, so that the thickness of the overall instrument is reduced, and the characteristic that POCT (Point Of Care Testing) detection is handheld and portable is reflected; an LED (Light-Emitting Diode) light source unit is arranged inside a shell of the diagnosis instrument, and the light emitted from the LED light source unit can be uniformly distributed on a detection card arranged in the fixing device, thus an effective image of the detection area can be rapidly captured by the imaging sensor.

The invention discloses a detection method and a kit for colorectal cancer cells based on quantum dot labeling. The detection method comprises steps of an anti-human M2-type pyruvate kinase monoclonal antibody preparation process, a quantum dot fluorescent probe preparation process and a process that quantum dot fluorescent probes are added in colorectal cancer cells and of fluorescence images of the colorectal cancer cells are observed under a confocal fluorescence microscope. Through anti-human-M2-type-pyruvate-kinase monoclonal antibodies labeled with quantum dots, a liquid chip or an immunofluorescence detection colorectal cancer cell or protein immunologic diagnosis kit can be formed. The kit has advantages of high sensitivity and high specificity, rapid and accurate detection can be achieved, secreta comprising feces and serum can be detected, and detection accuracy of the colorectal cancer can be raised greatly.

The invention belongs to the technical field of immunologic diagnosis, and in particular relates to a recombinant antigen composition for detecting a herpes simplex virus type-II IgG antibody. The recombinant antigen composition comprises a recombinant antigen in the epitope of the glycoprotein gG and a recombinant antigen in the epitope of the glycoprotein gD. The invention further discloses a detection kit comprising the recombinant antigen composition. The herpes simplex virus type-II IgG antibody is detected by adoption of the kit comprising the recombinant antigen composition in the invention; and thus, the detection accuracy is greatly improved.

The invention discloses an immunochromatographic test strip for fluorescent quantitative detection of PAPP-A (Pregnancy Associated Plasma Protein A) and a preparation method of the test strip, and belongs to the technical field of immunologic diagnosis. In order to overcome the defects in the prior art, the immunochromatographic test strip for the fluorescent quantitative detection of the PAPP-A adopts the following technical scheme that the test strip comprises a sample pad, a marker pad, a coating film and absorbent paper which are sequentially overlapped on a PVC (Polyvinyl Chloride) bottom plate; avidin is sprayed on the marker pad; a quality control line is coated with a rabbit anti avidin antibody for specific recognition of the avidin. The test strip has the following advantages that the test strip is used for evaluating and monitoring a level of the in-vivo PAPP-A, is simple to operate, quick, convenient, economic and accurately quantitative, and is more suitable for wide development in various medical institutions.