47 results about "Terminal deoxynucleotidyl transferase" patented technology

The invention relates to the field of biological detection and the field of nanomaterial functional application, and discloses a method for preparing a core-shell SERS structure based on the amplification of a nucleic acid strand by terminal deoxynucleotidyl transferases. The method comprises the following steps of: assembling a starting strand on the surface of a nanomaterial, carrying out the DNA amplification reaction on the surface of the terminal deoxynucleotidyl transferase, transferring deoxynucleotide to the starting primer strand assembled on the surface of the nanomaterial so as to form long single-stranded DNA on the surface of the nanomaterial and then obtain a nanomaterial-long-single-stranded-DNA complex; and covering a metal shell layer outside the nanomaterial-long-single-stranded-DNA complex by the chemical reduction method so as to form the core-shell structure. The core-shell SERS structure has long strand DNA which can be used as a Raman reporter small molecule so as to output efficient and uniform SERS signals to enhance the intensity of Raman signals. Bases are located between the core and the shell, so that the number of ''hot spots'' in the core-shell structure is increased, the adsorption of target molecules or antibodies on the surface of the core-shell structure is affected, the sensitivity of the detection can be greatly improved, and the method canbe applied to the field of biological analysis and detection.