The invention discloses a thermostatic strand displacement amplification technology. According to the technology, after heating denaturation is conducted on a target DNA sequence, a pair of primers containing RNA sequences are used for hybridizing with a template DNA single chain, the primers are extended under the action of strand displacement DNA polymerase, a combined sequence of target DNA andRNA containing an identification site of endoribonuclease is generated, the identification site is cut by the hydrolyzation of thermal resistant endoribonuclease to form viscous termini, the primersare connected with the viscous termini, a 3' terminus is extended under the action of the strand displacement DNA polymerase and replaces another DNA strand, a displaced strand is sequentially turnedinto target sources of another amplification reaction after the displaced strand is hybridized with the primers, the steps are constantly repeated in a reaction system, and the target DNA sequence isincreased in exponential rate. The technology is convenience, sensitive, rapid and good in specificity, the detecting lower limit is DNA with a plurality of gene copy numbers, the technical detect ofthermostatic amplification in the prior art is overcome, and the technology can be widely applied to the fields of clinical medicine, medical jurisprudence, animal and plant quarantine inspection andthe like.