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10811results about How to "Efficient detection" patented technology

Iterative probe design and detailed expression profiling with flexible in-situ synthesis arrays

Methods and compositions are provided that are useful for detecting and reporting a plurality of different target polynucleotide sequences in a sample, such as polynucleotides corresponding to a plurality of different genes expressed by a cell or cells. In particular, the invention provides methods for screening a plurality of candidate polynucleotide probes to evaluate both the sensitivity and the specificity with which each candidate probe hybridizes to a target polynucleoide sequence. Candidate polynucleotide probes can then be ranked according to both their sensitivity and specificity, and probes that have optimal sensitivity and specificity for a target polynucleotide sequence can be selected. In one embodiment, polynucleotide probes can be selected according to the methods described herein to prepare “screening chips” wherein a large number of target polynucleotide sequences are detected using a single microarray have a few (e.g., 1–5) probes for each target polynucleotide sequence. In a particularly preferred embodiment, the invention provides a screening chip that can detect genetic transcripts from the entire genome of an organism. In an alternative embodiment, polynucleotide probes can be selected according to the methods described herein to prepare “signature chips” to more accurately detect certain selected “signature genes” using several polynucleotide probes (e.g., 10–20) for each signature gene. The invention additionally provides microarrays containing polynucleotide probes for a large number of genes expressed by a cell or organism. Further, methods for detecting a plurality of polynucleotide molecules, including a large number of genes expressed by a cell or organism, are also provided.
Owner:MERCK & CO INC

Method and apparatus for detecting doubled bills in a currency handling device

A doubles detection system for detecting doubled documents. The system comprises one or more light sources disposed on a first side of a test document and one or more reflected light sensors disposed along the first side of the test document. The reflected light sensors are adapted to generate reflected light signals. The system also comprises one or more transmitted light sensors disposed along a second side of the test document which are adapted to generate transmitted light signals. The system also comprises a memory having a master reflected light value and a master transmitted light value stored therein. The system comprises a processor adapted to receive the reflected light signal, generate a reflected light value for the test document, and calculate a reflectance ratio between the reflected light value of the test document and a master reflected light value. The processor is also adapted to receive the transmitted light signal, generate a transmitted light value for the test document, and adjust the transmitted light value for the test document based on the reflectance ratio. The processor is adapted to compare the adjusted transmitted light value for the test document to the master transmitted light value and generate a doubles signal if the comparison of the adjusted transmitted light value for the test document with the master transmitted light value indicates that more than one document is present.
Owner:CUMMINS-ALLISON CORP
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