The invention provides a quantitative characterization method of low penetration double-medium sandstone oil reservoir microscopic aperture structure, which comprises the following steps: selecting experiment samples, effectively combining various experiments, distributing samples, processing and analyzing experiment test data, combining macroscopic background and microscopic rock core, and combining static state analysis and dynamic production reality, thereby realizing quantitative characterization of ultra-low penetration double-medium sandstone oil reservoir microscopic aperture structure from qualitative analysis and semi-quantitative evaluation. The invention has the advantages that more comprehensive influence factors and micro crack, aperture throat parameter and nuclear magnetic resonance movable fluid parameter are considered, so the characterization result can better reflect change characteristics of ultra-low penetration double-medium sandstone oil reservoir microscopic aperture structure, which are consistent to the production exploitation real cases of the oil field, thereby effectively avoiding one-sidedness and limitation of single aspect evaluation result.