The invention provides a method for rapidly establishing a phallus impudicus variety Donglin violet
tissue culture system through underground buds. According to the method, underground buds of 'Donglin purple' are used as explants, clean and tender underground buds ingeniously slip out through the methods of base
cutting, fixing and light clamping, cluster buds are directly induced after the propagation coefficient is increased through longitudinal
cutting, then roots are induced to form in-vitro plants, the sterile rate in 5 days is 38.48%, the fungal rate is 0.21%, the sterile rate in 25 days of primary subculture is 74.79%, and the rooting rate in 50 days is 87.12%. According to the method, the
callus induction stage is not needed, the time for obtaining the high-quality sterile Donglin purple in-vitro
plant is shortened, the technology is easy to master, the cost is low, the efficiency is high, and a foundation is laid for establishing a high-efficiency improved variety propagation
system through further subculture of cluster buds or induction of
callus redifferentiation subsequently. The invention provides a
solid guarantee for improved variety breeding, stem apex detoxification,
ploidy breeding and
genetic engineering genetic improvement research of iris varieties, especially phallus impudicus' Donglin purple '.