Primers, micro-fluidic chip and system for swine fever virus detection, and application of primers

A microfluidic chip, swine fever virus technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc. To solve the problem of high needle cost, to achieve the effect of improved detection convenience, improved analytical sensitivity and high accuracy

Pending Publication Date: 2020-06-12
宁波爱基因科技有限公司
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AI Technical Summary

Problems solved by technology

Molecular biology detection techniques include reverse transcription-polymerase chain reaction (RT-PCR), reverse transcription-mediated isothermal nucleic acid amplification technology (RT-LAMP) and fluorescent quantitative RT-PCR technology (Real-time RT-PCR) ), these techniques have the characteristics of high specificity and high sensitivity, but the fluorescein label of the fluorescent probe is easily decomposed when exposed to light, and needs to be stored away from light. The cost of synthesizing the fluorescent probe is relatively high, and the fluorescent PCR instrument is expensive Wait

Method used

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  • Primers, micro-fluidic chip and system for swine fever virus detection, and application of primers
  • Primers, micro-fluidic chip and system for swine fever virus detection, and application of primers
  • Primers, micro-fluidic chip and system for swine fever virus detection, and application of primers

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Embodiment

[0049] Find the target sequence through NCBI GenBank, and design primers for the target sequence. The designed primer sequences are shown in Table 1 below (the corresponding sequence list is provided with sequence order):

[0050] Table 1 Primer Sequence

[0051] P1 AGGGACTAGCCGTAGTGG P2 AGGTCGTACCCCCATCAC P3 GGTGAGCTTCTGCTCATGTCGA-AGCTCCCTGGGTGGTCTA P4 CGAGATGCTATGTGGACGAGGG-TGTGAGTTCACCCTAGCGA

[0052] And fix them on the corresponding positions of the microfluidic chip, then package the microfluidic chip, mix the reaction solution, reverse transcriptase, inhibitor with pork and its various organs, blood, excrement, environmental samples and After the nucleic acid template extracted from the cell culture is mixed and reacted, it is added to the packaged microfluidic chip, and then put into the microfluidic chip detector with centrifugal function, constant temperature function and real-time fluorescence detection, driven by centrifugal force ...

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Abstract

The invention discloses primers, a micro-fluidic chip and a system for detecting swine fever viruses, and application of the primers, wherein the primers are a primer group with the following sequences: P1:AGGGACTAGCCGTAGTGG, P2: AGGTCGTACCCCCATCAC, p3: GGTGAGCTTCTGCTCATGTCGA-AGCTCCCTGGGTGGTCTA, and P4: CGAGATGCTATGTGGACGAGGG-TGTGAGTTCACCCTAGCGA. According to the invention, the multiple defects ofLAMP isothermal amplification are thoroughly overcome, the detection effects of simple operation, rapid result output, multiple target points, synchronization and integration are realized, the characteristics of high sensitivity and high specificity are achieved, and a plurality of different indexes such as the swine fever virus and the like of the same sample or swine fever virus indexes of a plurality of samples can be detected at one time.

Description

technical field [0001] The invention relates to the technical field of swine fever virus detection, in particular to a primer for detection of swine fever virus, a microfluidic chip, a system and an application thereof. Background technique [0002] Microfluidics refers to the science and technology involved in the use of micropipes (tens to hundreds of microns in size) to process or manipulate tiny fluid systems. It is a discipline involving chemistry, fluid physics, microelectronics, new materials Emerging interdisciplines of , biology and biomedical engineering. Because of the characteristics of miniaturization and integration, microfluidic devices are usually called microfluidic chips, that is, various basic operating units (preparation, reaction, separation, detection, etc.) are integrated on a chip of several square centimeters. [0003] The current gene detection technology is based on PCR (polymerase chain reaction) technology and LAMP (loop-mediated isothermal nucl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/6844C12N15/11
CPCC12Q1/701C12Q1/6844C12Q2600/166C12Q2531/119C12Q2521/107C12Q2527/127C12Q2565/629C12Q2545/101C12Q2563/107
Inventor 刘艳红卢先东方雪恩
Owner 宁波爱基因科技有限公司
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