The invention discloses a colon cancer diagnostic marker and its application. The colon cancer diagnostic marker is a urine metabolite, including pseudouridine, N 2 ,N 2 ‑Dimethylguanosine, Kynurenine, Valine, N‑Acetyl‑L‑Valine, N‑Acetyl‑L‑Glutamate, 5‑Hydroxytryptophan, α,β‑ At least one of didehydrotryptophan, hippuric acid, aspartyl-phenylalanine, taurine, pantothenic acid, 2,3-pyridinedicarboxylic acid, 4-pyridoxic acid, L-carnitine, xanthine , based on the markers, the present invention also provides a colon cancer detection kit, which can effectively distinguish colon cancer patients from healthy people, with high accuracy, strong sensitivity and specificity. The sample selected in the present invention is a urine sample, preferably the middle part of fasting morning urine, which avoids the use of invasive samples such as tissue and blood, and has high patient compliance and clinical application value.