361results about How to "Wide detection range" patented technology

The invention discloses a bridge disease detection method and system based on an improved multi-mode visual language model, and the method comprises the steps: obtaining a historical bridge original image which comprises a bridge cantilever casting section, a cast-in-place section, a web, a diaphragm plate, a closure section and a concrete box girder; constructing an improved multi-modal visual language target detection model; based on the bridge original image, training the improved multi-modal visual language target detection model to obtain a trained improved multi-modal visual language target detection model as a bridge disease detection model; obtaining a to-be-detected bridge image, and dividing the to-be-detected bridge image into a plurality of overlapped image blocks based on an SAHI sliding window mechanism; detecting each image block in a local region based on the bridge disease detection model to obtain a region detection result; and on the basis of the detection result of each region, generating a global detection result in a splicing and fusion mode.

The invention discloses aggregation-induced emission microspheres with colorimetric-fluorescence double signals as well as a preparation method and application of the aggregation-induced emission microspheres. The aggregation-induced emission microsphere comprises a carboxyl polystyrene microsphere and an aggregation-induced emission dye embedded in the carboxyl polystyrene microsphere, the aggregation-induced emission microsphere has colorimetric-fluorescence double signals, is red under natural light, has strong absorption at 600nm, shows high luminous efficiency at 725nm, not only effectively avoids the interference of biological autofluorescence and reduces background interference, but also has good light stability and photobleaching resistance, and can be used for preparing the aggregation-induced emission microsphere. And due to the high molar extinction coefficient, the colorimetric and fluorescent double-signal output capability is realized at the same time. In addition, the aggregation-induced emission microspheres are high in reproducibility in the preparation process, have the potential of large-scale synthesis and meet the requirements of industrial batch production.

The invention discloses a sensing detection and drug delivery integrated platform, a needle tube array and a method. According to the method for integrally preparing the electrodes with the independent functions through dimension reduction machining, the complexity of a three-dimensional micromachining technology and mutual interference of the electrodes with the different functions in the machining process are effectively avoided. On the basis of a second-generation glucose sensing electrode, a mediator-enhanced micro needle tube sensing electrode is constructed, and high-sensitivity and wide-detection-range specific response to blood glucose fluctuation in subcutaneous tissue fluid is achieved. A micro-needle diagnosis and treatment integrated platform integrating sensing detection and a drug delivery module is constructed, a diabetes animal model is utilized, four key physiological parameters such as blood sugar, keto acid, lactic acid and pH in a subcutaneous microenvironment are explored in situ and monitored in real time, and a controllable drug delivery method is adopted, so that a safe, painless transdermal and drug delivery mechanism of the micro-needle tube array is further verified; and a new path is provided for detection, early warning and treatment of acute complications of diabetes mellitus.

The invention provides a system for evaluating an ADE effect in vitro and application of the system. The system comprises: (i) and (iii); (ii) and (iii); or (i), (ii) and (iii); in one embodiment, the present invention relates to a cell comprising: (i) an SARS-CoV-2 pseudovirus, (ii) an antibody that targets the receptor binding domain of SARS-CoV-2, and (iii) a cell that overexpresses CD32B; the cells are CHO cells. The system provided by the invention can be used for evaluating the ADE effect of the to-be-detected SARS-CoV-2 or the pseudovirus prepared from the to-be-detected SARS-CoV-2 or the antibody targeting the SARS-CoV-2, and has the advantage of higher sensitivity compared with a system based on cells expressing CD32A. The ADE evaluation system has a wide detection range, and can evaluate the ADE effect caused by the action of the SARS-CoV-2 virus or the pseudovirus prepared from the SARS-CoV-2 virus on CHO-CD32B in the presence of the RBD antibody with a wide concentration range (16 ng / mL-10000 ng / mL).

The invention discloses a preparation method of a bimetallic MnFe-N-C monatomic nano-enzyme and application of the bimetallic MnFe-N-C monatomic nano-enzyme in SCN <-> detection and dye degradation, and belongs to the technical field of SCN <-> detection and dye degradation. The preparation method specifically comprises the following steps: step (1), preparing a MnFe-N-C monatomic nano-enzyme material; (2) preparing a MnFe-N-C monatomic nano-enzyme material for colorimetric detection of SCN <->; step (3), preparing a MnFe-N-C monatomic nano-enzyme material degradation dye; and (4) preparing a colorimetric sensing working curve of the MnFe-N-C monatomic nano enzyme for detecting the thiocyanate. The bimetallic MnFe-N-C monatomic nano-enzyme is prepared by adopting a green and simple synthesis process, the cost is greatly reduced, the bimetallic MnFe-N-C monatomic nano-enzyme has the characteristics of high stability and high catalytic activity, SCN-is detected by a colorimetric method, and dye rhodamine B is rapidly degraded by activating peroxymonosulfate. The method has the advantages of higher sensitivity, wider detection range, higher detection speed, simplicity in operation and the like.

The invention discloses strength detection equipment for a special-shaped precast concrete component, which belongs to the technical field of building material detection and structurally comprises a suspension seat, a lifting frame, an L-shaped clamping arm and an edge strength detection rod. The suspension seat is connected to the lower side of a sliding table at the bottom of the portal frame, and L-shaped clamping arms are movably installed on the two sides of the lifting frame respectively. The end part of the clamping and supporting head is fixedly connected with a bearing rod, and the edge strength detection rod is detachably and fixedly mounted at the end part of the bearing rod; at least two cross-shaped strength detection rods are arranged below the suspension seat; the special-shaped concrete prefabricated component strength detection device can perform strength detection on the edge, the upper side of the bottom and the inner side of the side wall of the special-shaped concrete prefabricated component to verify whether irregular defects exist in the special-shaped concrete prefabricated component or not, is wide in detection range and high in comprehensiveness, controls the special-shaped concrete prefabricated component to perform multi-direction large-amplitude oscillation in space on the basis, and improves the detection accuracy. Various irregular vibrations possibly suffered in the construction environment are simulated, and whether the vibration meets the use requirements or not is verified.

The invention provides a power load and power grid interactive regulation and control method and system based on a big data technology, and belongs to the field of power load regulation and control. The problem of low power load regulation and control efficiency is solved; the method specifically comprises the following steps: S1, estimating the expected electricity consumption of each municipal district; s2, acquiring local power data, adjusting active power and reactive power of the regional power grid according to the expected power consumption and the local power data, and calculating a to-be-distributed ratio; s3, acquiring power transmission line data, and adjusting the transformation ratio of each transformer on the target line according to the to-be-distributed ratio and the power transmission line data; s4, continuously adjusting the power supply of the target area; according to the method, the power load and the power supply data of the target region are acquired, analyzed and processed, the power allocation of the target region is adjusted, and the power resource allocation is optimized.

The invention relates to the technical field of sensors, in particular to a self-adjusting flow sensor. Comprising a pipeline, a guide piece, a movable block, a limiting piece, a convergent-divergent pipe, a fluid director, a turbine and a frequency sensor. The diameter of the convergent-divergent pipe is automatically adjusted so as to adjust the sectional area of the pipeline, namely, the sectional area of the pipeline is large when the flow is large, the sectional area of the pipeline is reduced when the flow is medium, the sectional area of the pipeline is further reduced when the flow is small, the pressure intensity of liquid is increased, and meanwhile the impact part of the liquid to the turbine is guided according to the flow; the portion where the liquid impacts the turbine is far away from the axis, the turbine can rotate conveniently, and therefore the flow detection range is wide.

The invention discloses an impact resistance detection device and method for light guide film production, and relates to the technical field of film production. The device comprises a supporting frame and a light guide film which are arranged on a supporting base, and further comprises a positioning unit which is arranged on the supporting base and comprises a positioning assembly and a pushing assembly; the impact unit provided with an impact ball is arranged on the positioning unit and comprises a deflection assembly, an impact assembly and a distance adjusting assembly, the deflection assembly is used for adjusting the angle of the impact assembly, and the distance adjusting assembly is used for adjusting the stroke of the impact ball according to the deflection angle to achieve equal-force impact. The device has the advantages that multi-angle impact resistance detection can be carried out on the light guide film, the detection range is wider, the impact stroke of the impact ball is flexibly adjusted according to the inclination angle after the inclination angle is adjusted, the impact force of the impact ball impacting the light guide film at different inclination angles is kept the same, drop point deviation cannot occur under the action of gravity, and the detection accuracy is improved. And the detection result is more accurate.

The invention discloses a QPS numerical ray tracing and multi-target optimization-based high-frequency sky-ground wave radar ionosphere electron concentration inversion method, which comprises the following steps of: firstly, carrying out FFT (Fast Fourier Transform) on radar echo data, constructing a radar echo distance-Doppler matrix, and according to the obtained radar echo distance-Doppler matrix, calculating the electron concentration of a high-frequency sky-ground wave radar ionosphere according to the obtained radar echo distance-Doppler matrix; determining an antenna direct wave group distance and a transmitting station-receiving station ground distance; then determining an optimal emission elevation angle by using randomly generated ionosphere parameters; and finally, taking the emission elevation angle data corresponding to the n groups of ionosphere parameters as an initial population, and carrying out iterative optimization on the initial population by utilizing a population optimization algorithm to obtain an optimal ionosphere parameter result, thereby realizing ionosphere electron concentration inversion. According to the scheme, the radar echo data and the randomly generated ionosphere parameters are combined, the amount of data needing to be calculated is remarkably reduced, accurate inversion of the ionosphere parameters is achieved through ray tracing and multi-target optimization, the structure is simpler, the method does not depend on a complex model or a large amount of experience data, and the method is suitable for large-scale popularization and application. The optimal ionosphere parameter can be approached more quickly and effectively, and the real-time performance and accuracy of ionosphere electron concentration inversion are improved.

The invention discloses a construction method and application of a ratio-dependent electrochemical luminescence (ECL) biosensor based on an antifouling red cell membrane, and belongs to the technical fields of photoelectric analysis, biosensing and interface antifouling. In the invention, the erythrocyte membrane with super-hydrophilicity and low membrane protein content is used as an efficient antifouling interface, and the good biocompatibility of the erythrocyte membrane avoids the influence of a chemical antifouling coating on the biological activity of the ECL sensor. Meanwhile, the electropolymerized polyaniline nanowire is embedded into the antifouling erythrocyte membrane, so that the chemical stability and the conductivity of the antifouling erythrocyte membrane are enhanced, and the application of the antifouling erythrocyte membrane in ECL analysis is expanded. C3N4 is introduced as an interior label for eliminating background signals and external interference, so that the detection precision of the biosensor is improved. On the basis, the constructed ECL antifouling biosensor realizes ultra-sensitive detection of human breast cancer cells MCF-7 in human serum, and the detection limit is as low as 3 cells / mL.

The invention provides a hybrid creatine kinase isoenzyme double-antibody sandwich ELISA kit and a preparation method thereof, and belongs to the technical field of CK-MB antibody and creatine kinase isoenzyme quantitative detection. The process comprises the following steps: respectively immunizing Japanese big-ear rabbits and mice by using CK-MB heterodimer protein as an immunogen, screening positive monoclonal cells by adopting a Beacon single-cell screening platform, and preparing a rabbit (mouse) anti-human CK-MB gene engineering antibody; the double-antibody sandwich ELISA detection kit is established by further screening optimal reaction conditions. The detection method disclosed by the invention has excellent performance in the aspects of intra-batch and inter-batch repeatability, sensitivity and specificity, and is suitable for CK-MB detection in clinical and scientific research.

The invention relates to the technical field of perfluorinated compound detection, and discloses a method for full-automatic on-line rapid detection of perfluorinated compounds in water, which comprises the following steps: pretreating a water sample to obtain a sample to be detected, and placing the sample to be detected in a sample introduction disc of an on-line solid-phase extraction device; full-automatic online solid-phase extraction (Online SPE) enrichment and purification: automatically enriching and purifying the to-be-detected liquid in the step 1 by utilizing the Online SPE through an online solid-phase extraction small column, realizing alternate sample injection of double columns through valve switching, washing the other column during sample loading of one column, eluting a target object, and performing UPLC-MS / MS analysis. On the basis of a full-automatic on-line solid phase extraction-high performance liquid chromatography-tandem mass spectrometry technology, the method for full-automatic on-line rapid detection of the perfluorinated compounds in the water is successfully established in combination with an iRefx rapid screening intelligent working process, and the perfluorinated compounds in the water can be rapidly detected in a full-automatic on-line mode through the Online SPE and the iRefx rapid screening intelligent working process. Automatic sample injection, online enrichment and purification, intelligent judgment of sample concentration, overlimit sample redetection and accurate quantitative analysis are realized, and the detection efficiency is remarkably improved.

The invention discloses a crystal form judgment method of a silicon carbide single crystal polymorphic symbiotic region and application, and belongs to the technical field of silicon carbide material detection. The determination method comprises the following steps: (1) testing: carrying out Raman test on silicon carbide single crystals to obtain a test spectrogram, and classifying characteristic peaks in the test spectrogram into first crystal form Raman characteristic peaks and second crystal form Raman characteristic peaks according to Raman characteristic peaks of different crystal forms; and (2) judgment: judging that the crystal form at the test point is the first crystal form, a symbiotic crystal form of the first crystal form and the second crystal form or the second crystal form according to the ratio of the peak intensity of the Raman characteristic peak of the first crystal form to the peak intensity of the Raman characteristic peak of the second crystal form. According to the method, Raman is adopted to test the silicon carbide single crystal material, and the crystal form at a test point is judged according to a test spectrogram, so that the crystal form of a multi-type symbiotic region of the silicon carbide single crystal material can be accurately distinguished and quantitatively characterized, and the method can be used for large-scale detection of a silicon carbide single crystal rod.

The invention belongs to the technical field of biochemical sensing, and particularly relates to a sulfide colloidal quantum dot-based biosensor for exosome detection as well as preparation and application thereof. Transferring the sulfide colloidal quantum dot solution to a metal gate electrode of a high electron mobility transistor or a working electrode of a screen printing three-electrode to form a sulfide colloidal quantum dot film; coating the sulfide colloidal quantum dot film with an antibody protein modification layer, and closing non-specific active sites on the antibody protein modification layer by adopting bovine serum albumin; the antibody protein can be specifically bound with transmembrane protein corresponding to the surface of an exosome to be detected, so that the exosome to be detected is captured on the surface of the electrode, and meanwhile, based on the unique physical and chemical effect of the sulfide colloidal quantum dots, the modified electrode has the capability of sensing the distribution change of external charges, so that the detection sensitivity of the exosome to be detected is improved. Therefore, rapid and quantitative detection of the exosome is realized.

The invention relates to a primer group, a method and a kit for detecting various modification gene variations influencing beta-thalassemia (beta-thalassemia for short) phenotypes. The method comprises the following steps: (1) a collection module: obtaining and preparing a subject sample; (2) an amplification module: carrying out long fragment multiple PCR (Polymerase Chain Reaction) amplification on BCL11A, ZBTB7A, KLF1, HBS1L-MYB, GATA1, HBG1 / 2, HBBP1, GATAD2A, DNMT1 and CHD4 in the sample; (3) a library construction module: constructing a single-molecule long-read sequencing library; and (4) a sequencing module for sequencing and analyzing gene variation types. The kit comprises the following reagents: (1) a reagent for long-fragment multiplex PCR amplification; and (2) a reagent for constructing a single-molecule long-read sequencing library.

The invention relates to a primer group and a kit for detecting Fabry disease GLA gene mutation and application of the primer group and the kit. The primer group comprises one or more of a GLA-F1 / R1 primer pair, a GLA-F2 / R2 primer pair, a GLA-F3 / R3 primer pair, a GLA-F4 / R4 primer pair, a GLA-F5 / R5 primer pair, a GLA-F6 / R6 primer pair and a GLA-F7 / R7 primer pair. The kit further comprises a GLA-Gap primer. Based on a specific combination of long-fragment PCR amplification and third-generation high-throughput sequencing, the method can be used for simultaneously detecting various mutations related to the Fabry disease pathogenic gene GLA of a plurality of samples in a high-specificity, accurate and rapid manner.

The invention relates to an expanded gate type field effect transistor sensing array and a preparation method and application thereof.The expanded gate type field effect transistor sensing array comprises a silk-screen printing electrode array and a metal oxide semiconductor field effect transistor, the silk-screen printing electrode array comprises a silver electrode and a carbon electrode, and the metal oxide semiconductor field effect transistor comprises a gate, a source and a drain. The carbon electrode is used as a detection probe after being modified by a gold / metal organic framework composite nano material and a detection probe based on a double-recognition strategy, and the carbon electrode is connected with the grid electrode through a wire to obtain the expanded grid type field effect transistor sensing array. Compared with the prior art, the novel extended gate type field effect transistor sensor for rapidly and sensitively detecting antibiotics is simple in structure, low in cost, high in stability and reusable. The double-recognition strategy probe based on combination of the aptamer and the molecularly imprinted polymer is prepared, the detection sensitivity and selectivity are improved, the synthesis method is simple, and experiment conditions are mild.

The invention discloses a preparation method and application of a tough, anti-freezing and high-conductivity composite hydrogel electrode, and the method comprises the following steps: 1, adding nanocellulose CNF into deionized water to obtain a uniform dispersion solution; step 2, stirring at room temperature to dissolve to obtain a uniform and clear precursor solution; 3, rapidly stirring to obtain a mixed solution; and 4, adding N, N '-methylene bisacrylamide (MBA) and ammonium persulfate (APS) into the mixed solution prepared in the step 3, stirring for 15 minutes to obtain a hydrogel precursor solution, uniformly stirring, carrying out ultrasonic defoaming, putting into a drying oven, and reacting at 60 DEG C for 4 hours to prepare the tough, anti-freezing and high-conductivity hydrogel. The method has the beneficial effect that the conductivity of the hydrogel is enhanced. The method has the advantages of high sensitivity, high stability, wide detection range and the like. Weak physiological signals can be monitored, and the system can be used for muscle electricity monitoring, electrocardiogram monitoring or motion monitoring.

The invention discloses table tennis anti-deformation detection equipment, and particularly relates to the technical field of anti-deformation detection, the table tennis anti-deformation detection equipment comprises a support frame, a miniature push electric cylinder, a detection pressure sensor and a multi-point synchronous extrusion detection mechanism; the multi-point synchronous extrusion detection mechanism comprises a sleeving sliding strip, a groove ring, a plurality of hinge blocks, a connecting shaft, a sleeving push rod, a push shaft, a connecting block and a sleeving block; meanwhile, the table tennis anti-deformation detection equipment further comprises a linkage extrusion assembly, a positioning twisting mechanism and an extrusion linkage mechanism. Through the multi-point-position synchronous extrusion detection mechanism and the linkage extrusion assembly, covering type compression resistance detection on multiple point positions of the outer wall of the table tennis ball is achieved, the accuracy of compression resistance detection of the table tennis ball is greatly improved, and therefore the problems that large-area covering detection on the multiple point positions of the outer wall of the table tennis ball is difficult to achieve due to single-direction detection, and the detection accuracy is poor are solved. Therefore, the detection accuracy of the table tennis ball is poor.

The invention relates to the technical field of circuit board detection, and discloses a circuit board blind hole detection device for a new energy automobile and a detection method thereof.The circuit board blind hole detection device comprises a chassis, the chassis is provided with a conveying mechanism, and the conveying mechanism comprises a rotating shaft rotationally connected to the upper portion of the chassis and can convey a circuit board; the circuit board detection device is simple in structure, convenient to use, capable of conveying a plurality of circuit boards and high in conveying efficiency, the blind hole detection mechanism is arranged, the lead performance of blind holes can be detected, the diameters of the blind holes can be detected, the detection efficiency is high, the circuit boards of different sizes can be detected, the detection range is wide, and the weighing mechanism is arranged, so that the detection efficiency is high. According to the circuit board weighing mechanism, the weight of a circuit board can be weighed through movement of the mechanism, whether the weight of the circuit board is qualified or not is judged, and the qualified circuit board and the unqualified circuit board can be output differently through the output mechanism.

The invention discloses a vehicle-mounted GNSS (Global Navigation Satellite System) multi-interference source monitoring and direction-finding device and a monitoring and direction-finding method, the device comprises a monitoring and direction-finding antenna and a monitoring and direction-finding host, the monitoring and direction-finding antenna comprises an antenna unit, a switch matrix, an electronic compass and an antenna controller, the antenna unit is electrically connected with the switch matrix, and the switch matrix is electrically connected with the electronic compass. The switch matrix and the electronic compass are electrically connected with the antenna controller respectively; the monitoring direction-finding host comprises a radio frequency receiving module, a signal processing module, a main control module and a power supply module. The monitoring and direction finding device disclosed by the invention is high in maneuverability, and can be carried on a vehicle platform to realize rapid monitoring and troubleshooting of interference sources in a certain area; the monitoring range is wide, satellite navigation interference signals in the vehicle driving range can be monitored in real time, and the detection range is wider than that of traditional handheld interference source monitoring and direction finding equipment.

The invention discloses a laser gas telemetering imaging system based on micro galvanometer scanning and a detection method, the system comprises: a laser transmit-receive optical unit, detection laser emitted by a semiconductor laser and aiming laser emitted by an aiming laser are combined by a beam combiner, and are collimated to an MEMS micro galvanometer through a collimation system; the MEMS micro galvanometer deflects by a corresponding angle to reflect the laser beam to a target detection area; the photoelectric detector array is used for receiving a light return signal of the detection laser; the signal processing and control unit is used for calculating the concentration information of the target gas in the target detection area according to the return light signal subjected to photoelectric conversion; the visual imaging unit is used for shooting a visual image of the detection area; and the information fusion unit is used for fusing the calculated gas concentration of the target detection area into a visual image, and sending the fused visual image to the signal output display unit for visual display of the gas concentration.

The invention discloses an aptamer electrochemical biosensor based on an AuNPs / MXene nanometer material and a preparation method and application of the aptamer electrochemical biosensor. According to the electrochemical biosensor, AuNPs particles grow on the surface of MXene, an AuNPs / MXene composite material is prepared, then the composite material is fixed to the surface of an electrode through chitosan, aptamer probes P1 and P2 are hybridized with MCH, and then the aptamer probes P1 and P2 are self-assembled to the surface of the electrode through Au-S bonds. According to the invention, the AuNPs / MXene nano material is prepared, and the AuNPs / MXene nano material and the AuNPs / MXene nano material play a synergistic effect, so that the performance of the sensor is further improved, the sensor has a wide detection range and a low quantitative line, and rapid, sensitive and specific detection on a sample can be realized. The sensor can be used for in-situ detection of inflammatory factors at a wound, can be applied to non-invasive instant wound nursing monitoring equipment, and provides reliable and timely monitoring data to realize more effective diagnosis and treatment.

The invention belongs to the technical field of millimeter wave frequency multiplication, and provides a millimeter wave high-order frequency comb frequency multiplier and a preparation method and application thereof. The millimeter wave high-order frequency comb frequency multiplier comprises a substrate layer and an absorption layer which are arranged in a stacked mode. The absorption layer comprises niobium-iridium-tellurium layers and metal electrode layers, parts of the metal electrode layers are in lap joint with the niobium-iridium-tellurium layers, and the four metal electrode layers are symmetrically distributed in the transverse direction and the longitudinal direction. According to the invention, niobium-iridium-tellurium is adopted as the absorption layer, and niobium-iridium-tellurium is a zero-band-gap material, so that the detection spectrum range is wide; meanwhile, niobium-iridium-tellurium is a non-centrosymmetric material and has a relatively large Bell curvature dipole coefficient, so that the niobium-iridium-tellurium frequency multiplier has a nonlinear Hall effect induced by the Bell curvature, and the millimeter wave high-order frequency comb frequency multiplier constructed by the niobium-iridium-tellurium frequency multiplier has a high-order frequency multiplication effect, does not need additional bias voltage and can stably work at room temperature and low temperature. In addition, dependence on a high-frequency millimeter wave-terahertz source is effectively reduced, and a theoretical basis is provided for development of the high-frequency millimeter wave-terahertz source.

The invention discloses a device and a method for detecting grouting fullness of a concrete prefabricated wallboard, and relates to the technical field of grouting fullness detection of prefabricated wallboards. The device comprises a plurality of grouting sleeves which are pre-buried in a prefabricated wallboard and provided with grouting pipes and grout outlet pipes, and further comprises a detection cylinder, a gas detection unit is arranged in the detection cylinder, and a gas injection assembly and a gas return assembly are arranged in the gas detection unit; the control unit is arranged outside the detection cylinder; and a shock excitation hammer used for impacting the prefabricated wall panel is arranged in the impact detection unit. The method has the advantages that by adopting a nondestructive detection mode of detecting the air pressure change of the upper part through lower part air exhaust and detecting the air collection content of the upper part through lower part air blowing, the interior of the grouting sleeve is not influenced during detection, the fullness of the slurry in the cured grouting sleeve can be accurately calculated, and the detection accuracy is improved by adopting a detection mode of detecting echoes through multi-position hammering. The internal compactness of the grouting sleeve and the position of a cavity can be visually detected, and the detection range is wider.

The invention belongs to the field of intelligent aerogel, and discloses intelligent aerogel capable of being repeatedly used for indicating volatile biogenic amine and a preparation method of the intelligent aerogel. An anthocyanin natural extract is used as a specific color-sensitive responder of volatile biogenic amine, a naturally degradable cellulose material and a hydrophilic polymer are used as base materials of the intelligent aerogel, an edible plasticizer is used as a processing aid, and water is used as a solvent system of the material. And preparing the aerogel material for packaging the anthocyanin by adopting a directional freezing-vacuum freeze drying process. The intelligent aerogel prepared by the preparation method disclosed by the invention has the application advantages of being recyclable, high in indication (detection) sensitivity, low in detection limit, quick to use, high in use safety and capable of instantly identifying the existence and the concentration of the volatile biogenic amine by naked eyes.

The invention belongs to the field of marine environmental protection, and relates to a primer and a probe for detecting toxigenic Pseudonitzschia polyspora, application of the primer and the probe, a kit and a detection method. The invention relates to primers for detecting toxin-producing pseudonitzschia multistriata, the primers comprise an upstream primer and a downstream primer, the upstream primer is Dab AA5F 5 '-GATCCSTTCAACTRGTCTTYATTTCA-3', and the downstream primer is Dab AA5R 5 '-CATAYCRAATAAVGAWTCTTCTKGGTC-3'. The specific primer and the probe provided by the invention can be used for detecting and monitoring the offshore toxin-producing pseudonitzschia multistriata in China, and have the advantages of high specificity, high sensitivity, simplicity and convenience in operation, rapidness in detection, wide detection range and the like.

The invention belongs to the technical field of detection, and particularly relates to a universal kit for bovine nodular skin viruses, sheep pox viruses and goat pox viruses. On the basis of expressed specific recombinant p122 protein and serum to be detected as a detection sample, the double-antigen sandwich ELISA method for the bovine nodular skin virus, the sheep pox virus and the goat pox virus is established, and the method comprises the following steps: coating an antigen, sealing, adding the sample to be detected and an enzyme-labeled antigen, developing, judging a result and the like. The kit has the advantages of being good in specificity, high in sensitivity, good in repeatability, low in cost and capable of achieving batch detection, and a certain detection technology foundation can be laid for preventing and controlling the bovine nodular skin virus, the sheep pox virus and the goat pox virus.

The invention relates to the technical field of sensors, in particular to a strain sensor based on a multi-layer wrinkled structure and a preparation method and application thereof.The sensor simulates a skin wrinkled structure and belongs to a capacitive sensor, the sensor comprises a flexible medium insulating tape and two layers of flexible layered film electrodes, the flexible layered film electrodes are respectively positioned on two sides of the flexible dielectric insulating tape and cover the flexible dielectric insulating tape, and the flexible layered film electrodes are formed by combining a platinum-modified copper nanowire film (Cu (at) Pt film) with a flexible substrate material polyvinylidene fluoride (PVDF) as a conductive layer. The sensor has high sensitivity and a wide stretching range, overcomes the defects in existing material selection, and has potential application in the fields of exercise monitoring, health monitoring and information transmission.