171results about How to "Good fluorescence stability" patented technology

The invention discloses a near-infrared silver sulphide quantum dot as well as a preparation method and application thereof. The silver sulphide quantum dot has a monoclinic phase structure, the particle diameter of the silver sulphide quantum dot is below 8nm, and the surface of the silver sulphide quantum dot can be connected and modified with a hydrophilic group of hydrophilic reagent containing sulfydryl. The preparation method comprises the following steps: fully reacting a mixed reaction system containing silver source and long-chain mercaptan in a sealed environment; cooling the mixed reaction system, and then adding a polar solvent; carrying out centrifugation and wshing to obtain a hydrophobic near-infrared silver sulphide quantum dot; fully mixing the hydrophobic near-infrared silver sulphide quantum dot and hydrophilic reagent containing sulfydryl in organic solvent to react; and carrying out centrifugation and washing the reacting mixture to obtain the biocompatibility near-infrared silver sulphide quantum dot. The quantum dot disclosed by the invention has the advantages of high fluorescence yield, good fluorescence stability, good biocompatibility and even size, and can be used for biological tissue imaging; and the preparation technology has moderate condition, short period and good repeatability, is simple to operate and is easy to control.

The invention discloses a method of detecting the residue of small-molecule substances harmful to the human body and a special kit. The special kit comprises a non-transparent micro-porous plate and a light-emitting compound, wherein each hole of the non-transparent micro-porous plate is filled with a coat antigen which is simultaneously coated with three kinds of small-molecule substances. The invention makes full use of the multi-color marking function of QDs, establishes a novel kit for simple and rapid detection of the residue and a method thereof, and realizes the multi-color marking through indirect marking of polyclonal antibodies and monoclonal antibodies in the veterinary drug by coupling the QDs with different particle sizes and targets with functional groups (such as an amino) with specific surfaces. The method comprises: obtaining quantum dots with different fluorescent characteristics through separation and purification, namely, multi-color antibody markers, using the multi-color antibody markers as fluorescent probes, and establishing a reaction system for synchronous analysis of various antigen components of different kinds, thereby realizing the synchronous detection of multiple kinds of residues of the veterinary drug in animal food. Moreover, the method has the advantages of simple operation, high fluorescence intensity and long stabilization time.

The invention relates to magnetic fluorescent microspheres and a preparation method thereof. The particle size of the magnetic fluorescent microspheres is 5-10 mu m, the fluorescence excitation wavelength range is 400-700nm, and the fluorescence intensity is not reduced within 24h. The prepration method comprises the following steps: adding a swelling agent into monodisperse carboxylated polystyrene microspheres, and adding magnetic nano microparticles into a swelling system; shaking on a decolorization shaker for 12-48h; using mixed solution of cyclohexane and ethanol for cleaning sediment, and sequentially carrying out ultrasonic dispersion and centrifugal separation till supernatant liquid is colorless under an ultraviolet lamp; and saving a final sediment product in 1ml of liquor. Compared with the existing magnetic fluorescent microspheres, the magnetic fluorescent microspheres have the advantages of uniform and controllable diameter, good fluorescence stability, simple preparation process, multiple types of fluorescence codes and the like, and can not only carry out fast separation and purification on reactants by being applied in the biological macromolecular detection, but also simultaneously detect a plurality of target molecules in a sample to be detected in a reaction tube and a hole, thereby being widely applied in the fields of immunoassay, nucleic acid hybridization, genotype analysis and the like.

The invention discloses a magnetic fluorescent multimodal nano biological probe as well as a preparation method and application thereof. The magnetic fluorescent multimodal nano biological probe specifically targets a liver-cancer cell. According to the preparation method, magnetic nano particles and fluorescent dye are co-embedded in silicon dioxide; an amino group is functionally modified by the silicon dioxide; nucleic acid aptamer and a magnetic fluorescent probe are covalently coupled and synchronously specifically target the liver-cancer cell. The magnetic nano particles are paramagnetic iron oxides of which the sizes are ten nanometers; the fluorescent dye is fluorescein isothiocyanate; the embedding medium silicon dioxide is obtained through the hydrolysis of tetraethyl orthosilicate and 3-aminopropyltriethoxysilane; the nucleic acid aptamer is TLS11a. A synthetic method of the probe is simple and convenient; materials are easily obtained; fluorescence properties are stable; a foundation is laid for the early diagnosis of a liver cancer.

The invention relates to a method for preparing a Y2O3:Tb<3+>@SiO2-NH2 fluorescence sensor array used for detecting an organic chlorine pesticide. The marking recognition sites in the fluorescence sensor array are mutually effected with organic chlorine pesticide molecules (target molecules), the target molecules and Tb<3+> on the recognition sites form an organic ligand through coordination, and change of the fluorescence intensity of Tb<3+> is used for detecting the target molecules. The method comprises the following three steps: preparing Y2O3:Tb<3+> fluorescent powder, then synthesizing a Y2O3:Tb<3+>@SiO2-NH2 fluorescence probe having capability for identifying the marking target molecules, finally, through a micromachining technology and a plasma etching method, dropping the fluid suspension of the fluorescence probe having the capability for identifying the marking target molecules on a silicon chip, naturally drying the silicon chip, and spontaneously putting the fluorescence probe materials in a micro hole to obtain the fluorescence sensor array for high-selectivity identification and high sensitive signal detection on the target molecules.

The invention discloses a novel method for rapidly detecting aflatoxin in food. The method comprises main steps as follows: an existing precolumn derivatization technology and post-column derivatization technology for aflatoxin are combined, precolumn derivatization is performed under the irradiation of ultraviolet light, a derivative reaction is performed completely, produced fluorescence is strong, the fluorescence stability of a derived product is good, and meanwhile, the detection sensitivity is also improved. Compared with methods for measuring aflatoxin in existing standards, the method has the characteristics that the detection sensitivity is high, post-column light derivatization equipment is not required, the reproducibility is good, the detection is accurate and the like.

The invention provides a preparation method of a highlight blue-green fluorescent water soluble silicon quantum dot. The highlight blue-green fluorescent water soluble silicon quantum dot is synthesized through one step at normal pressure by adopting a high boiling point water soluble reagent glycerol as a solvent and utilizing a microwave method. The preparation method of the highlight blue-green fluorescent water soluble silicon quantum dot comprises the following steps: adding sodium citrate into glycerol, and dissolving thoroughly; introducing Ar gas for removing oxygen in a solution, then adding 3-aminopropyl triethoxy silane, and continuously stirring while the Ar gas is introduced; transferring the solution into a microwave reactor, and reacting for 5-15 minutes at the temperature of 160-200 DEG C; adding the obtained silicon quantum dot solution into absolute ethyl alcohol, centrifuging, and reserving supernatant; and dialyzing the supernatant by adopting a dialysis bag, so as to obtain a pure silicon quantum dot solution, and carrying out freeze drying on the pure quantum dot solution for later use. The preparation method of the highlight blue-green fluorescent water soluble silicon quantum dot has the advantages that synthesis is realized through one step at normal pressure by adopting the microwave method, a synthetic process is simple and easy to operate, and the synthesized water soluble silicon quantum dot has the advantages of long emission wavelength, high luminous intensity, good fluorescent stability, low cytotoxicity and good biocompatibility.

The invention relates to a copper selenide fluorescent quantum dot and a preparation method and application thereof. The copper selenide fluorescent quantum dot is characterized in that the quantum dot is one of Cu2Se, CuSe and Cu(2-x)Se or a combination thereof; the particle size of the quantum dot is 2-10nm, the quantum yield is 10-60%, and the half-peak width of the photoluminescence spectrum is 30-150nm. The preparation method comprises the following steps: with an aqueous solution with copper salt dissolved in as an aqueous phase and an organic solvent with a long-chain ligand dissolved in as an oil phase, stirring in an inertia protective gas, and heating in an oil bath to form a precursor solution; pouring an aqueous solution of sodium hydrogen selenide into the hot precursor solution, and heating for reaction; sampling at different time to obtain organic-phase quantum dot solutions with different emission fluorescence; precipitating the upper-layer organic-phase quantum dot solution; centrifuging and dispersing, and repeating to remove unreacted raw materials to obtain the copper selenide fluorescent quantum dot. The quantum dot does not contain heavy metal elements such as cadmium and the like to realize low toxicity, has the advantages of high quantum yield, uniform particle size, relatively narrow fluorescence emission spectrum and adjustable light color along with excitation wavelength, and can be used for preparing quantum dot solar sensitized cells and LED (light-emitting diode) devices.

The invention discloses fluorescent nanospheres, a preparation method thereof, and applications of the fluorescent nanospheres in the field of chemical sensing. The fluorescent nanospheres, which are fluorescent nanoprobes and are uniform in particle size distribution, are obtained via catalytic auto-polymerization of 3-aminophenylboronic acid in an aqueous solution at the presence of a catalyst, and via ultrafiltration processing. The fluorescent nanoprobes can be combined with adenosine monophosphate-modified oxidized grapheme so as to form a fluorescence resonance energy transfer system; when cis-dyhydroxy biological molecules are added into the fluorescence resonance energy transfer system, because of competition on boron affinity interaction, the fluorescent nanospheres and the adenosine monophosphate modified oxidized grapheme are separated, and combination of the fluorescent nanospheres with the cis-dyhydroxy biological molecules is realized, fluorescence of the fluorescent nanospheres is recovered, and in a certain concentration range, fluorescence intensity and the concentration of the cis-dyhydroxy biological molecules are in a linear relationship. Fluorescence properties of the fluorescent nanoprobes are stable, and are not easily influenced by environmental factors. The preparation method is simple; identification selectivity and capacity of resisting disturbance of the fluorescent nanoprobes are excellent.

The invention discloses a method for detecting the concentration of ferrohemoglobin by using a fluorescent carbon dot probe. The method comprises the following steps: 1, preparing different concentrations of fluorescent carbon dot-containing ferrohemoglobin standard solutions, detecting the fluorescence intensity of the standard solutions to obtain a standard solution fluorescence spectrogram, and establishing a linear relation between a difference between the fluorescence intensity of the standard solution with the ferrohemoglobin concentration being 0 and the fluorescence intensity of every standard solution and the ferrohemoglobin concentrations; and 2, preparing a fluorescent carbon dot-containing ferrohemoglobin sample solution, detecting the fluorescence intensity of the sample solution, and determining the concentration of ferrohemoglobin in the sample solution through the linear relation. The ferrohemoglobin concentration is detected by using the characteristics of a ferrohemoglobin quenching fluorescence carbon dot with the fluorescence carbon dot as a probe, so the method has the advantages of simple and convenient detection process, high sensitivity and low detection limit, and can realize online, in-situ, rapid and sensitive detection of the concentration of ferrohemoglobin in a practical sample.

The invention provides a fluorescent probe based on MOF, a preparation method and an application of same, belongs to the technical field of fluorescence sensors, and is used for solving problems detection of concentration of hippuric acid in the prior art has complex operations, long time consumption and low sensitivity. The preparation method includes the steps of: A) adding metal ions, containing Al<3+>, and 2-aminoterephthalic acid to deionized water, uniformly mixing and stirring the solution, performing a reaction at 120-160 DEG C to prepare MOF solid powder; B) adding the MOF solid powder, carboxylated calix[4]arene, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide to deionized water, and stirring the liquid to produce the fluorescent probe based on MOF. The invention also provides an application of the fluorescent probe based on MOF as a fluorescence sensor in detection of hippuric acid. The fluorescent probe has high fluorescent emitting efficiency and good fluorescent stability, is convenient and quick to use in detection and has high sensitivity and good stability.

The invention discloses a nickeliferous fluorescent quantum dot which is low in toxicity, has wide absorption spectrum and narrow fluorescence emission spectrum, and is adjustable in luminous color along with excitation wavelength, high in luminous efficiency, high in stability and applicable to construction of quantum dot-sensitized solar cells and LED devices. The nickeliferous fluorescent quantum dot comprises one of or combination of nickel selenide, nickel telluride and nickel sulfide, the quantum dot particle size ranges from 2nm to 20nm, the quantum yield is 10-60%, and the half-peak width of a photoluminescence spectrum is 30-130nm. Preferably, the NiSe fluorescent quantum dot is optimal when x=0.5.

The invention discloses a method for detecting potassium permanganate through graphene quantum dot fluorescence quenching. The method is characterized in that a graphene quantum dot probe is used as a fluorescence probe and by virtue of the characteristic that the fluorescence intensity of the graphene quantum dot probe weakens with the increasing of the concentration of a potassium permanganate solution, high-sensitivity detection is performed and the change value of the fluorescence intensity of the graphene quantum dot probe forms a good linear relationship with the concentration of the potassium permanganate, with the correlation coefficient of 0.991. The method is easy and convenient to operate, rapid in detection, high in sensitivity and good in selectivity, and can perform online in-situ rapid and sensitive detection on the potassium permanganate in a mixed sample.

The invention relates to a preparation method of a multifunctional dyed fluorescent cotton fabric based on MOF. The method includes the steps of preparing a pretreated Zn-MOF cotton fabric and preparing the multifunctional dyed fluorescent cotton fabric based on the Zn-MOF. The method is simple in process operation and high in efficiency and requires no special equipment, raw materials are low incost and easy to obtain, and the method is suitable for industrial production. The prepared multifunctional dyed fluorescent cotton fabric based on the Zn-MOF is excellent in fluorescent performance and meanwhile has the properties of resisting bacteria, preventing generation of stink, mold and moths, resisting ultraviolet rays, retarding inflaming and the like.

The invention relates to the field of detection of cultural relics and discloses a method for identifying the varieties of residual silk of antique silk fabric based on an immune trace method. The method comprises the following steps: preparing a fluorescent carbon point by taking a sweet potato as a carbon source, marking secondary antibodies of mulberry silk and tussah silk by using the fluorescent carbon point, hydrolyzing promiscuous antique silk fabric cultural relic samples by using ionic liquid and PM13-alkaline protease step by step, performing dialysis and purification after protein extracting liquid is obtained, performing SDS-PAGE gel electrophoresis, transferring the obtained protein band to a PVDF membrane, incubating silk primary antibodies and the secondary antibodies marked by the fluorescent carbon point, observing the immunofluorescence band in a gel imaging system, and identifying the varieties of the antique silk fabric. According to the method, the use amount of a chemical reagent is small, and the reaction is mild, environment-friendly and harmless; and when the antique silk fabric is detected, the method has the characteristics of small use amount of samples, intuitive performance, rapidness and high sensitivity.

The invention discloses quantum-dot fluorescent encoded microspheres based on fluorescence resonance energy transfer analysis. The quantum-dot fluorescent encoded microspheres are characterized in that quantum-dot fluorescent encoded polystyrene microspheres are 2-10 mu m in diameter; the surface of the microspheres is modified with functional groups; three or more types of CdSe@ZnS core-shell quantum dots emitting different wavelengths are embedded in the microspheres; the microspheres can emit the wavelength of 500-600 nm and have the particle size of about 2-10 nm. Amino groups are introduced to the surface of the quantum-dot fluorescent encoded microspheres; after chemical and biological modifications are carried out, the microspheres can be used to construct sensitive elements for liquid chips, so that high-throughput high-specificity analytic detection is achieved for biological macromolecules, such as enzymes and proteins.

The invention relates to a preparation method for carbon quantum dots stable in pH and strong in light emission. The preparation method includes the steps of calcining sodium glutamate in the N2 atmosphere to obtain carbon dot powder, dissolving carbon dots, conducting ultrasonic oscillation, filtering and drying, and dissolving and dialyzing the dried product to obtain the carbon quantum dots. The preparation method is easy and convenient to operate, free of high-pressure environments and easily industrialized. The carbon quantum dots prepared through the method have the advantages of being stable in pH and light emission for the environment, high in quantum efficiency, free of light blinking phenomena, uniform in size, extremely high in stability, high in biocompatibility, and the like.

The invention relates to a method for microwave-assisted preparation of a CdTeSeS / ZnTe core-shell quantum dot in water. The method comprises the following steps: 1) preparing a sodium elenosulfate solution; 2) preparing a cadmium chloride solution; 3) adding mercaptopropionic acid into the cadmium chloride solution; 4) adjusting the pH value of the solution; 5) dissolving a sodium tellurite crystal in the solution and simultaneously adding potassium borohydride and the sodium elenosulfate solution; 6) subjecting a mixture obtained in the step 6) to heating reaction in a microwave digestion furnace; 7) carrying out cooling to room temperature and adding isopropanol for purification so as to obtain CdTeSeS quantum dot gel; and 8) preparing a mixed solution of the cadmium chloride solution, the mercaptopropionic acid and the sodium tellurite crystal, dissolving the CdTeSeS quantum dot gel in the mixed solution anew, adding potassium borohydride and successively carrying out heating reaction in the microwave digestion furnace, cooling to room temperature, addition of isopropanol for purification and drying with a lyophilizer so as to obtain solid powder of the CdTeSeS / ZnTe core-shell quantum dot. The method is fast and simple and has easily controllable technological parameters and a low price; and the synthesized quantum dot has uniform particle size distribution, high stability, high fluorescence quantum yield and wide emission spectrum.

The invention belongs to the fluorescent sensing technical field and specifically relates to a high-efficiency organic fluorescent material and application of the material in TNT (trinitrotoluene) detection. The structural formula of the high-efficiency organic fluorescent material provided by the invention is Ar2-Ar1-Ar-Ar1-Ar2, wherein Ar1-Ar-Ar1 forms a light-emitting unit; the light-emitting center Ar can be spirofluorene, benzene, carbazole (alkyl chains different in length can be connected at No.9 site) or fluorene (alkyl chains different in length can be connected at No.9 site); Ar1 can be fluorene, biphenyl or difluorene; and electric-active unit Ar2 can be furan, pyrrole, thiophene, carbazole, diphenylamine or triphenylamine. An electro-polymeric thin film formed from the material through electrochemical polymerization is characterized in that intermolecular aggregation in the electrochemical polymerization process can be effectively reduced, the fluorescence efficiency of the electro-polymeric thin film is improved, and the electro-polymeric thin film is capable of effectively transferring charges with TNT molecules to quench fluorescent light, and therefore, effective detection on TNT is realized.

The invention discloses a method for preparing fluorescence precise adjustable perovskite nanocrystals by using a metal halogenated inorganic salt aqueous solution as an anion exchange reagent, and belongs to the technical field of preparation of semiconductor nanocrystals. The method is characterized in that metal halogenated inorganic salt is dissolved into water and is fully ionized, so that the aims of promoting the reaction activity of halogen ions and accelerating the anion exchange reaction rate are achieved. According to the method, the addition amount of the metal halogenated inorganic salt aqueous solution in an oil phase solution of perovskite nanocrystals is quantitatively controlled; in addition, the water-oil two-phase reaction is promoted by using ultrasound, so that continuous and accurate regulation of a fluorescence emission spectrum at a nanometer interval in the full spectral range is realized. The method disclosed by the invention has the advantages that the disadvantages of complex synthesis, low phase purity, low reaction rate, high toxicity and the like of an existing anionic exchange method are effectively overcome; the perovskite nanocrystals with preciseadjustable full spectral fluorescence can be accurately, easily and conveniently prepared in batch.

The invention provides a preparation method of a fluorescent carbon quantum dot. The method comprises the following steps: a, taking sorbose as a carbon source, adding the sorbose into a mixed solution of ethanol and water, and fully carrying out mixing, wherein the ratio of the sorbose to the mixed solution of ethanol and water is (0.5-4g):50mL, and the volume ratio of the ethanol to the water is1:(1-3); b, putting the mixed liquor obtained in the step a into a reaction kettle, carrying out a hydrothermal reaction for 2-8 hours at a temperature of 170-200 DEG C, carrying out cooling to roomtemperature after the reaction is finished to obtain a reaction liquid; c, filtering the reaction liquid, and placing the filtered reaction liquid in a dialysis bag for dialysis for 1-3 days to obtaina water solution of the target carbon quantum dot; and d, carrying out vacuum drying on the water solution of the carbon quantum dot to obtain the target carbon quantum dot. According to the preparation method, the sorbose is taken as a carbon source, so that the preparation method has the advantages of simple synthesis process, easy control of conditions and the like, and the prepared carbon quantum dot has good fluorescence stability and wide adjustable range of light-emitting wavelength.

The invention discloses a method for detecting potassium dichromate by utilizing a graphene quantum dot probe. The method is characterized by comprising the following steps: (1) establishing a linear relationship: preparing a standard solution of graphene quantum dot and potassium dichromate solution, and obtaining the linear relation between the fluorescent intensity of the graphene quantum dot and the concentration of the potassium dichromate through the fluorescent spectra; (2) detecting: detecting the fluorescent intensity of the to-be-detected potassium dichromate solution, and determining the content of the potassium dichromate in the to-be-detected potassium dichromate solution according to the linear relation. The method is easy to operate, rapid in detection, high in sensitivity and good in selectivity, has the characteristics of high sensitivity and high selectivity when potassium dichromate in a mixed sample is detected and is promising in application prospect.

The invention relates to a method for detecting hemoglobin based on GQDs (graphene quantum dots). The method comprises two main points as follows: (1) establishment of a linear relation: the fluorescence intensity of the GQDs is weakened with an increase of the concentration of a hemoglobin solution according to a fluorescence spectrogram, and a fluorescence quenching value and the concentration of the hemoglobin solution have a good linear relation; (2) detection of a simulation sample: the fluorescence intensity of a to-be-detected hemoglobin solution is detected, and the content of hemoglobin in the to-be-detected hemoglobin solution is determined according to the linear relation. Meanwhile, the method can also be applied to content detection of hemoglobin in a mixed system, and a result proves that the method has the advantage of high-sensitivity detection, thereby having a broad application prospect.

The invention discloses a nuclear acid probe marked with quantum-dots composite sphere and a preparation method and application thereof. Nuclear acid probes with different sequences are marked with quantum-dots composite sphere with different emission spectrum characteristics; the quantum-dots composite sphere comprises a sphere and a quantum-dots composite contained in the sphere, the quantum-dots composite is composed of randomly selected m types of quantum-dots with different emission spectrum characteristics from n types thereof, n and m are both positive whole numbers and m is less than or equal to n; the preparation method of the nuclear acid probe comprises the following steps: preparing numerous probes with different sequences and marking the probes with X; preparing numerous quantum-dots composite sphere with different emission spectrum characteristics and marking the sphere with Y; integrating probes marked with X and quantum-dots composite sphere marked with Y through specificity of X and Y and carrying out coupling on the probes and sphere; applied to multicolor fluorescence in situ hybridization, spectral karyotype analysis and chromosome karyotype analysis, the probeof the invention can improve sensitivity, repetitiveness and specificity of detection, simplify detection device, increase detection speed and prevent false positive result from occurring.

The invention discloses a fluorescent CdSe nanometer quantum dot probe for pesticide dimethoate, and application of the probe. A preparation method of the fluorescent CdSe nanometer quantum dot probe for pesticide dimethoate comprises the steps of preparing selenium stock solution; preparing cadmium stock solution; and preparing the fluorescent CdSe nanometer quantum dot probe for pesticide dimethoate. The probe has detection limit up to 1.5*10<-7> mol / L for pesticide dimethoate. The preparation method has the advantages of simple operation method, rapid detection, and high sensitivity and selectivity for dimethoate in a mixed sample.

The invention discloses a method for detecting potassium dichromate and ascorbic acid by using a graphene quantum dot probe. The method is characterized in that the graphene quantum dot probe is used as a fluorescence probe and by virtue of the characteristics that the fluorescence intensity of the graphene quantum dot probe weakens with the increasing of the concentration of a potassium dichromate solution and increases with the increasing of the concentration of an ascorbic acid solution, high-sensitivity detection is performed and the change value of the fluorescence intensity of the graphene quantum dot probe forms a good linear relationship with both the concentration of the potassium dichromate and the concentration of the ascorbic acid, with the correlation coefficients of 0.994 and 0.996 respectively. The method is easy and convenient to operate, rapid in detection, high in sensitivity and good in selectivity, and can perform online in-situ rapid and sensitive detection on the potassium dichromate and the ascorbic acid in a mixed sample.

The invention provides a preparation method and application of fungal hypha nitrogen and sulfur self-doped carbon dots and belongs to the field of researches of fluorescent carbon nano-materials. Thepreparation method comprises the following preparation steps: (1) firstly, preparing fungal hyphae by utilizing a biosynthesis technology; culturing the hyphae in a PDA (Potato Dextrose Agar) solid culture medium at constant temperature; then inoculating the hyphae into a modified PDA liquid culture medium and culturing in a constant-temperature oscillator; finally, purifying the hyphae; and (2) taking the hyphae as a raw material and synthesizing the nitrogen and sulfur self-doped carbon dots (N,S-CDs) in one step under a hydrothermal condition. According to the preparation method provided bythe invention, a method combining biosynthesis and the hydrothermal condition is adopted for the first time, and the N,S-CDs with excellent performance are prepared. The N,S-CDs can be used for carrying out high-selectivity and high-sensitivity detection on tetracycline antibiotics (TCs); and when the concentration of TC is 0.5 to 38.46muM, the linear correlation coefficient is 0.991 and the TC detection limit is 0.041muM. The N,S-CDs are used for preparing fluorescence detection test paper and the fluorescence detection test paper can be used for rapidly detecting the TCs in culture wastewater. The N,S-CDs do not influence the survival rate of cells and have a very great application potential in the field of cell imaging.

The invention discloses a preparation method of a hydroxyl modified metal organic framework material fluorescent probe, and fluoride detection application of the fluorescent probe, and belongs to thetechnical field of fluorescent sensing. A hydroxyl modified metal organic framework material (MIL-53(Al)-OH) is prepared by taking aluminium nitrate hexahydrate as a metal salt ligand and 2,5-dihydroxyterephthalic acid as an organic ligand in an N,N-dimethylformamide solvent through solvothermal reaction. The material is ultrasonically dispersed in Tris-HCl buffer solution, so that the fluorescentprobe MIL-53(Al)-OH-Tris can be prepared; under the 365 nm excitation wavelength, the MIL-53(Al)-OH-Tris fluorescent probe has a strong fluorescence emission peak at 515 nm; and thus, the fluorescentprobe can be used for sensitive detection of fluoride ions.

This invention refers to a method for preparing micro array biochip for fluorescent detection, made from SiO2 mesoporous assembling CdS and ZnS semiconductor nano crystal. Said method comprises preparing order mesoporous SiO2 film, connecting mesoporous assembling coupling agent, assembling of CdS or ZnS in mesoporous and activation of assembling substrate.

The invention discloses a preparation method of a copper nanocluster self-assembly and application of the copper nanocluster self-assembly as a fluorescent probe to detection of histamine. 2,3,5,6-tetrafluorothiophenol serves as a ligand and a reductant to synthesize the banded copper nanocluster self-assembly with orange fluorescence in one step. The preparation method is a novel method for preparing the copper nanocluster self-assembly and has the advantages of being simple to operate, quick in preparation and low in cost. The synthesized copper nanocluster self-assembly has the advantages of broad excitation spectrum, narrow emission spectrum, high fluorescence quantum yield, strong fluorescence stability and the like, and besides, can be used for detecting histamine.