Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method and application of fungal hypha nitrogen and sulfur self-doped carbon dots

A self-doping and hyphae technology, applied in chemical instruments and methods, nanotechnology for materials and surface science, nanotechnology, etc., to achieve low cytotoxicity, simple preparation technology, and easy operation

Inactive Publication Date: 2019-04-09
NORTHEAST FORESTRY UNIVERSITY
View PDF5 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there have been many literatures reporting the doping of heteroatoms in CDs, but most of them require the modification of foreign chemical reagents.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and application of fungal hypha nitrogen and sulfur self-doped carbon dots
  • Preparation method and application of fungal hypha nitrogen and sulfur self-doped carbon dots
  • Preparation method and application of fungal hypha nitrogen and sulfur self-doped carbon dots

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The preparation method and application of the nitrogen and sulfur self-doping carbon dots of fungal hyphae of the present invention are specifically carried out according to the following steps: the fungal mycelium is synthesized by biosynthesis technology. The fungal hyphae were cultured in the PDA solid medium at a constant temperature of 25°C. After the mycelium covered the surface, the above-mentioned mycelium was inoculated in the PDA liquid medium, and placed in a 160r / min constant temperature shaker for 25 Cultivate at ℃ for 10 days, and finally purify the fungal hyphae with dilute lye with a concentration of 0.5mol / L, boil for 30 minutes, wash with deionized water until neutral, freeze-dry for later use; (2) use the above-mentioned biosynthesized fungi Take silk as raw material, take 2g and add it into 25mL deionized water, stir magnetically for 30min to form a homogeneous mixture; (3) transfer the mixture to a 50mL hydrothermal reactor, and react in a homogeneou...

Embodiment 2

[0016] Using biosynthesis technology to synthesize fungal hyphae. The fungal hyphae are cultivated at a constant temperature under 25°C environmental conditions in the PDA solid medium, and the surface is covered with the mycelia, and then the above-mentioned mycelium is inoculated in the modified PDA liquid medium (the mass ratio of the amino acid to the PDA liquid medium is 1 :15), placed in a constant temperature oscillator at 120-160r / min, cultivated at 25-30°C for 6-14 days, and finally purified the fungal hyphae with dilute lye, washed with deionized water until neutral, and freeze-dried for later use ; With the above-mentioned biosynthetic fungal hyphae as raw material, 1 g was added to 25 mL of deionized water, and magnetically stirred for 30 min to form a uniform mixture; React at ℃ for 6 hours; after the reaction is completed, cool it down naturally to room temperature, then centrifuge the obtained dark brown liquid at 8000rpm for 15 minutes to remove large particles...

Embodiment 3

[0018] Using biosynthesis technology to synthesize fungal hyphae. The fungal hyphae are cultivated at a constant temperature under 25°C environmental conditions in the PDA solid medium, and the surface is covered with the mycelia, and then the above-mentioned mycelium is inoculated in the modified PDA liquid medium (the mass ratio of the amino acid to the PDA liquid medium is 1 :10), placed in a constant temperature oscillator at 120r / min, cultivated at 30°C for 5 days, and finally purified the mycelium of the fungus with dilute lye, washed with deionized water until neutral, and freeze-dried for later use; the above biosynthesized Fungal hyphae as raw material, take 1.5g and add it to 25mL deionized water, and stir it magnetically for 30min to form a uniform mixture; transfer the mixture to a microwave oven and react in the microwave oven at 100°C for 5min; After cooling down to room temperature, the obtained dark brown liquid was centrifuged at 8000 rpm for 15 min to remove ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a preparation method and application of fungal hypha nitrogen and sulfur self-doped carbon dots and belongs to the field of researches of fluorescent carbon nano-materials. Thepreparation method comprises the following preparation steps: (1) firstly, preparing fungal hyphae by utilizing a biosynthesis technology; culturing the hyphae in a PDA (Potato Dextrose Agar) solid culture medium at constant temperature; then inoculating the hyphae into a modified PDA liquid culture medium and culturing in a constant-temperature oscillator; finally, purifying the hyphae; and (2) taking the hyphae as a raw material and synthesizing the nitrogen and sulfur self-doped carbon dots (N,S-CDs) in one step under a hydrothermal condition. According to the preparation method provided bythe invention, a method combining biosynthesis and the hydrothermal condition is adopted for the first time, and the N,S-CDs with excellent performance are prepared. The N,S-CDs can be used for carrying out high-selectivity and high-sensitivity detection on tetracycline antibiotics (TCs); and when the concentration of TC is 0.5 to 38.46muM, the linear correlation coefficient is 0.991 and the TC detection limit is 0.041muM. The N,S-CDs are used for preparing fluorescence detection test paper and the fluorescence detection test paper can be used for rapidly detecting the TCs in culture wastewater. The N,S-CDs do not influence the survival rate of cells and have a very great application potential in the field of cell imaging.

Description

technical field [0001] The invention relates to fluorescent carbon nanomaterials, in particular to a method for preparing fungal hyphae nitrogen-sulfur self-doping carbon dots, and applying the prepared fungal hyphae nitrogen-sulfur self-doping carbon dots to TCs detection and cell imaging. Background technique [0002] With the increasing demand for aquatic products, high-density and intensive aquaculture methods have gradually formed. Antibiotics are widely used to prevent and treat fish diseases or to promote fish growth and reproduction, especially tetracycline antibiotics, as a broad-spectrum antibiotic, are widely used in aquaculture for the treatment of Gram-positive and negative bacteria Infect. Tetracycline antibiotics mainly include TC, CTC, OTC and doxycycline (DOXC), and the first three are the most used in aquaculture. Inappropriate use of tetracyclines can lead to unsafe levels of residues, and residues can contribute to increased bacterial resistance. There...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/65B82Y20/00B82Y30/00G01N21/64
CPCG01N21/6428G01N21/643C09K11/65B82Y20/00B82Y30/00G01N2021/6432
Inventor 黄占华石彩戚后娟马荣秀
Owner NORTHEAST FORESTRY UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com