934 results about "Liquid culture" patented technology

The invention belongs to the technical field of biological material engineering, and particularly relates to a fungal imitation leather material and a preparation method thereof. The method comprises the following steps: firstly, adopting wild fomes fomentarius subjected to 60Co irradiation mutagenesis as a production strain of a mycelium pad, and screening and optimizing components and content of a liquid culture medium to obtain a surface culture liquid culture medium suitable for the wild fomes fomentarius; secondly, by utilizing the strain and an optimized culture medium, the low-cost and large-scale production of the mycelium pad can be realized under the optimal culture condition; and the mycelium pad is subjected to various physical and chemical treatments such as plasticizing, cross-linking, hot-pressing compaction and the like, so that the mechanical strength of the mycelium pad is further improved, and the mechanical property requirements of leather products are met.

The invention discloses a needle mushroom bacterial liquid culture reaction kettle, and relates to the technical field of bacterial liquid culture preparation equipment.The needle mushroom bacterial liquid culture reaction kettle comprises a rotating assembly, the rotating assembly is fixedly connected with a motor, the outer side of the rotating assembly is fixedly connected with a round pipe, square holes are formed in the outer side of the round pipe, and the square holes are evenly distributed with the round pipe as the center; a fixing ring is rotatably connected to the end, away from the rotating assembly, of the circular pipe, a rotating plate is fixedly connected to one end of the fixing ring, a supporting rod is fixedly connected to the end, close to the rotating plate, of the fixing ring, a reset spring is fixedly connected to the end, close to the circular pipe, of the supporting rod, and a baffle is slidably connected to the outer side of the supporting rod. According to the flammulina velutipes bacterial liquid culture reaction kettle, a culture solution and strains in the tank body are stirred in multiple degrees of freedom and different directions through rotation of the round pipe and the rotating plate, so that the strains and the culture solution are uniformly mixed, and the quality of culture production of the strains is ensured.

The invention relates to the field of microorganisms, in particular to penicillium fox CK152 and application thereof. The invention provides a strain of Penicillium faecium CK152, and the preservation number of the Penicillium faecium CK152 is CGMCC (China General Microbiological Culture Collection Center) NO.41452. The Penicillium faecium CK152 can be used for preparing a strain of Penicillium faecium CK152. Experimental verification shows that a liquid culture of the penicillium fox CK152 strain provided by the invention has a remarkable inhibition effect on magnaporthe oryzae, the bacteriostasis rate of the liquid culture can reach 90%, and the inhibition rate of the liquid culture on magnaporthe oryzae spore germination is 100%. Therefore, the invention provides a new strain which has an excellent control effect on the pathogenic fungi, and the new strain has important significance on the control of the pathogenic fungi.

The invention discloses a cordyceps militaris mycelium fermentation product as well as a preparation method and application thereof, and belongs to the technical field of daily cosmetics. A preparation method of a cordyceps militaris mycelium fermentation product comprises the following steps: taking cordyceps militaris mycelium as a strain, inoculating the strain into a liquid culture medium, and carrying out enlarged culture to obtain a seed solution; transferring the seed solution into a fermentation tank containing a liquid culture medium, introducing oxygen, stirring, and fermenting for a certain time to obtain a fermentation solution; adding an industrial compound enzyme into the fermentation liquid, and heating and stirring to obtain an enzymolysis treatment liquid; adding a certain amount of alcoholic solution into the enzymolysis treatment liquid, and fully and uniformly mixing to obtain alcohol precipitation liquid; and separating the alcohol precipitation liquid, filtering the supernatant, and collecting the clarified filtrate to obtain the cordyceps militaris mycelium fermentation product. The specific compound enzyme which is low in price and suitable for industrialization is adopted, active substances of cordyceps militaris are fully released, and the effect of the product in cosmetics is improved.

The invention relates to the field of piggery tail end odor pollution treatment, and discloses a piggery tail end odor purification filter element preparation method and application, the preparation method comprises the following steps: loading a photocatalyst on the surface of a filter element body to obtain a carrier, respectively inoculating 3-5 kinds of antagonism-free deodorization microorganisms into corresponding LB liquid culture mediums for activation and propagation, and carrying out purification on the LB liquid culture mediums to obtain the piggery tail end odor purification filter element. The preparation method comprises the following steps: preparing a bacterial solution, mixing the bacterial solution to obtain a corresponding bacterial solution, compounding all the bacterial solutions in proportion to obtain a compound bacterial solution, infiltrating a carrier in the compound bacterial solution, and carrying out biofilm formation at room temperature to obtain the filter element. After the photocatalyst on the surface is illuminated, some refractory pollutants can be decomposed into smaller intermediate products which are easier to utilize by microorganisms by utilizing light energy, a more suitable substrate is provided for the microorganisms, and the microorganisms can further thoroughly decompose the intermediate products into harmless substances such as carbon dioxide and water, so that the degradation effect is improved. Therefore, the overall degradation efficiency and degree of pollutants are improved.

The invention relates to the technical field of microorganisms and particularly discloses bacillus velezensis for producing tetramethylpyrazine and application of the bacillus velezensis, the preservation number of the strain is CCTCC NO: M20232058, and the bacillus velezensis is obtained by separating and purifying from high-temperature Daqu of Maotai-flavor liquor in the liquor industry of Jin-brand Maotai Town in Guizhou. The bacillus velezensis disclosed by the invention can produce 1875.4 mg / L of tetramethylpyrazine by performing shake flask fermentation on a liquid culture medium for 7 days, and the tetramethylpyrazine can reach 4810.8 mg / L by optimizing a culture medium formula and culture conditions. The bacillus velezensis is applied to small-scale brewing of Maotai-flavor liquor, the content of tetramethylpyrazine in the Maotai-flavor liquor reaches 34.5 + / -1.4 mg / L and is increased by 325.93% compared with a contrast, and the bacillus velezensis can be used for increasing the content of tetramethylpyrazine in the Maotai-flavor liquor.

The invention relates to the field of microbial genetic transformation, and discloses a preparation method of Ilyonectria fungus protoplast and a construction method of a genetic transformation system.The preparation method comprises the following steps that S11, Ilyonectria fungus hyphae are prepared, Ilyonectria fungi are cultured on a PDA solid culture medium for 14 d, then the Ilyonectria fungi are inoculated to a CMC liquid culture medium to be subjected to shake-flask culture for 4 d and filtered, filtrate is centrifuged for 10 min at the speed of 10000 rpm, and spores are collected; diluting the concentration of spores to 107 pieces / mL, inoculating the spores into a YEPD liquid culture medium, carrying out shake-flask culture for 2 days, and filtering and collecting hyphae; and S12, preparing the Ilyonectria fungus protoplast, namely adding the hypha prepared in the step S11 into the enzymatic hydrolysate, performing enzymolysis in a shaker at the temperature of 30 DEG C at the rotating speed of 150-200rpm for 3-5 hours, filtering, centrifuging filtrate at the temperature of 4 DEG C, discarding supernate, adding 0.7 M NaCl for centrifuging, and discarding the supernate, thereby obtaining the Ilyonectria fungus protoplast. The genetic transformation constructed by the invention can be used for exploring the specific action and mechanism of a specific pathogenic gene in the pathogenic process of Ilyonectria fungi through gene knockout or overexpression experiments.

A method for enhancing hydrogen uptake by cells of an acetogenic bacterium in a culture is provided. The method comprises incubating the cells in a liquid medium supplemented with an external gas mixture, wherein the external gas mixture comprises H2 and CO2; overexpressing an uptake hydrogenase and an associated electron transfer protein in the cells; increasing uptake of the H2 by the cells; and enhancing uptake of the CO2 by the cells. The culture may be a coculture with additional cells of a non-acetogenic bacterium, which produces natively H2 from catabolism of an organic molecule.

The invention discloses a method for improving generation of antagonistic substances of beneficial bacteria by utilizing bacteriophages. Under the continuous stress of the bacteriophage YL-Ste-01P, the yield of the bacteriostatic metabolic substances of the beneficial bacterium YL-Ste-01 is obviously increased. A bacterial wilt biocontrol bacterium YL-Ste-01 and a bacteriophage YL-Ste-01P are inoculated into an NA liquid culture medium for co-culture to obtain a culture solution, the culture solution is centrifuged to obtain supernate, and the supernate is filtered by a filter membrane to obtain fermentation liquor. According to the present invention, the co-culture fermentation broth of YL-Ste-01 and YL-Ste-01P, alpha-ketoglutaric acid, guanidinopropionic acid and fumagillin have significant inhibition effects on ralstonia solanacearum; the invention discloses application of an antagonistic substance in preparation of a reagent for inhibiting ralstonia solanacearum or prevention and control of bacterial wilt. The invention discloses application of alpha-ketoglutaric acid, guanidinopropionic acid or fumagillin to preparation of a reagent for inhibiting ralstonia solanacearum or prevention and control of bacterial wilt.

The invention discloses a fermentation method of recombinant bacillus subtilis for expressing vibrio parahaemolyticus outer membrane protein, which comprises the following steps: inoculating a recombinant bacillus subtilis strain into a liquid culture medium for activation and amplification to obtain a seed solution; transferring the seed solution into a fermentation tank according to the inoculum size of 5%-10%, controlling the temperature to be 35-37 DEG C and the pH to be 6.8-7.2, introducing sterile air and stirring, and monitoring the thallus concentration OD600 value in real time; when the OD600 value reaches 35-38, 10% of an inducer is added for pre-induction; when the OD600 value reaches 40-45, the remaining 90% of the inducer is added for main induction, and the real-time dissolved oxygen value is collected; when the real-time dissolved oxygen value is continuously higher than a first preset threshold value, the flow acceleration rate of the inducer is increased, the fermentation temperature is increased at the same time or step by step, and the increase amplitude of the dissolved oxygen value is in positive correlation with the flow acceleration rate and the temperature increase amplitude; when the OD600 value is stabilized in a preset interval, it is judged that fermentation is completed, and the recombinant bacillus subtilis thalli are obtained. The yield and quality of the vibrio parahaemolyticus outer membrane protein can be effectively improved.

The invention belongs to the technical field of preparation of probiotic products, and relates to a lactic acid bacteria microcapsule as well as a preparation method and application thereof. The method comprises the following steps: uniformly mixing sodium alginate, modified montmorillonite and sterile water, cooling, adding a prepared lactic acid bacteria seed solution, and fully and uniformly mixing to form an emulsion; spraying the emulsion into a calcium chloride solution by adopting an extrusion method, stirring and curing to obtain microcapsules; and culturing the prepared microcapsule in a lactic acid bacteria liquid culture medium to obtain the lactic acid bacteria microcapsule after the culture is finished. The lactobacillus is effectively protected by adopting an extrusion-method single coating and post-coating culture process, so that the stability of the lactobacillus to a humid and hot environment and the gastrointestinal stress resistance are improved, and meanwhile, the release performance of the lactobacillus in the gastrointestinal tract is ensured. The problem that an existing microcapsule coating technology cannot achieve balance between improvement of the hygrothermal stability and gastrointestinal stress resistance of the probiotics and guarantee of good release performance of the probiotics in the gastrointestinal tract is solved.

The invention relates to a method for producing notoginsenoside and ginsenoside by utilizing panax notoginseng cotyledon embryos, and belongs to the technical field of plant adventitious root tissue culture. The method comprises the following steps: inducing embryogenic calluses by utilizing immature zygotic embryos of panax notoginseng; proliferating the panax notoginseng embryogenic callus on a plate culture medium; s2, putting the pseudo-ginseng embryogenic callus obtained in the step S2 into a liquid culture medium, carrying out cell dispersion, and sieving by using a sieve with the aperture of 24-30 meshes; a mechanical stirring type bioreactor is filled with a liquid culture medium, and the globular embryo and the heart-shaped embryo are placed in the mechanical stirring type bioreactor to be cultured; s3, recovering the filtrate in the step S3, filtering by using a screen with the aperture of 30-80 meshes and 80-150 meshes to obtain uniform panax notoginseng embryogenic cells, and differentiating the panax notoginseng embryogenic cells in a triangular flask for the step S4; and drying the cotyledon embryo obtained in the step S4 at 45 DEG C, and grinding into powder. According to the method, large-scale generation of cotyledon embryos can be completed only in 4-6 weeks, the production process is remarkably accelerated, the method is suitable for industrial batch production requirements, and the production efficiency is high.

The invention provides a culture method for increasing the content of a bioactive substance Emodic acid in talaromyces purpurascens, and relates to the technical field of medicinal fungus culture. The culture method of the homopurple talaromyces spp. Comprises the following steps: carrying out liquid culture on hyphae of the homopurple talaromyces spp., and then inoculating the hyphae into a liquid culture medium containing black bean powder for culture. The defects in the prior art are overcome, and the pharmacological activity of the talaromyces purpurascens is enhanced by optimizing the formula of the fermentation culture medium and improving the fermentation conditions, so that the content of Emodic acid in the talaromyces purpurascens is increased.

The embodiment of the invention provides a method for converting ginsenoside in ginseng adventitious roots, which comprises the following steps: (a) taking liquid-cultured ginseng adventitious roots as raw materials, taking the ginseng adventitious roots out of a liquid culture medium, cleaning and removing the residual liquid culture medium on the surfaces of the ginseng adventitious roots, and removing moisture on the surfaces of the ginseng adventitious roots; and (b) suspending the ginseng adventitious roots obtained in the step (a) in high-temperature and high-pressure equipment, adding water into the bottom of the high-temperature and high-pressure equipment, heating to generate high-temperature and high-pressure steam to carry out high-temperature and high-pressure conversion on the ginseng adventitious roots, and controlling reaction parameters to obtain finished ginseng adventitious roots with the rare ginsenoside content obviously improved.

The invention discloses a method for rapidly detecting bacterial drug resistance by combining a bacterial metabolism fingerprint spectrum after short-time antibiotic stimulation with machine learning. Bacteria are incubated in a liquid culture medium containing antibiotics with break point concentration for a short time, the metabolic pathway of the bacteria can be changed, and then generation of downstream metabolites is affected. As the drug-resistant bacteria have a special drug-resistant mechanism, the change of the metabolic state of the drug-resistant bacteria is different from that of the sensitive bacteria. Analyzing the metabolite fingerprint spectrum of the bacteria after short-time stimulation of the antibiotics by adopting matrix-assisted laser desorption ionization time-of-flight mass spectrometry; after data acquisition, classified analysis is performed through machine learning, so that rapid detection of the antibiotic resistance of bacteria is realized. Compared with the prior art, the method has the advantages that bacterial drug resistance detection can be completed within 2 hours, high practicability and operability are achieved, and meanwhile the method has the advantages of being high in throughput and low in experiment cost.

The invention belongs to the technical field of microorganisms and fermentation engineering, and particularly relates to application of bifidobacterium longum subsp. Infantis Y46 in the aspects of enhancing cell defense and repair and regulating metabolic homeostasis of a host. The strain is isolated from infant feces. Animal experiments prove that living cell thalli of the bifidobacterium longum subsp. Infantis Y46, which are obtained by culturing an MRS liquid culture medium added with L-cysteine hydrochloride (0.05%, v / v), can significantly enhance the oxidative stress resistance of an organism and reduce the level of active oxygen in the organism compared with a control bacterium bifidobacterium longum subsp. Infantis 15697. Transcriptomics analysis shows that the bifidobacterium longum subsp. Infantis Y46 can enhance self-protection of cells and improve physiological imbalance caused by external pressure by regulating related signal channels of an organism. The action mechanism is closely related to signaling pathways related to cell defense, metabolic homeostasis and barrier functions. The invention provides a new strain resource and an application scheme for developing functional food or medicinal preparations for enhancing the health defense function of a human body.

The invention provides a culture method for increasing the content of antroquinonol in antrodia camphorata mycelia, and relates to the technical field of medicinal fungus culture. The culture method comprises the steps that antrodia camphorata mycelia are inoculated into a liquid culture medium added with meliaceae cedrela sinensis tender leaf homogenate to be cultured, the liquid culture medium is a PDA culture medium, and the adding amount of the meliaceae cedrela sinensis tender leaf homogenate is 0.5-2 g / 100 mL. According to the method, the defects in the prior art are overcome, the content of antroquinonol in the antrodia camphorata mycelia is increased by optimizing the formula of the fermentation culture medium and improving the fermentation conditions, and the pharmacological activity of the antrodia camphorata mycelia is enhanced.

The invention discloses a method for inducing sugarcane to resist sugarcane smut, and belongs to the technical field of sugarcane disease resistance. The method disclosed by the invention comprises the following steps: (1) obtaining sugarcane smut Pep1 gene knockout mutants PepMAT-1 and PepMAT-2; (2) culture of a sugarcane smut Pep1 gene knockout mutant: activating PepMAT-1 and PepMAT-2, and then inoculating the activated PepMAT-1 and PepMAT-2 to a YePS liquid culture medium for shake cultivation; and (3) inducing sugarcane to resist smut: after shake cultivation, mixing the PepMAT-1 and the PepMAT-2, and inoculating the mixture on sugarcane seedlings. The method can rapidly and efficiently induce sugarcane to resist sugarcane smut, and provides technical support for prevention and treatment of sugarcane smut.

The invention belongs to the technical field of microorganisms and fermentation engineering, and particularly relates to application of bifidobacterium longum subsp. Infantis Y46 in the anti-aging aspect. The bifidobacterium longum subsp. Infantis Y46 is obtained from excrement of infants. Animal experiments prove that living cell thalli (Y46) and thermally inactivated cell thalli (HKY46) obtained by culturing the bifidobacterium longum subsp. Infantis Y46 by using an MRS liquid culture medium containing L-cysteine hydrochloride (0.05%, v / v) can significantly reduce accumulation of lipofuscin in a host, and prolong the healthy life and life of the host. Therefore, the bifidobacterium longum subsp. Infantis Y46 is a promising probiotic and a promising metagen with an anti-aging function.

The invention belongs to the technical field of genetic engineering of edible mushrooms, and particularly relates to a construction method of an efficient and stable genetic transformation system of flammulina velutipes. The construction method comprises the following steps: culturing in an MM culture medium by taking a flammulina velutipes strain 0747 as a receptor; the pFgnpt vector is converted into agrobacterium AGL1 (AGL1); inoculating hyphae into an IM liquid culture medium containing acetosyringone and MES, mixing with agrobacterium tumefaciens, culturing, and transferring the hyphae to an IM solid flat plate paved with filter paper; hyphae are transferred to a PDA resistance plate containing G418 and cephalosporin for resistance screening, and an efficient and stable flammulina velutipes genetic transformation system is obtained. The new selection marker G418 is developed and applied, limitation of a traditional selection marker is broken, more choices are provided for flammulina velutipes genetic transformation, a new thought is provided for genetic transformation research of other edible mushrooms, and the application field of an agrobacterium tumefaciens-mediated transformation technology is expanded.

The invention discloses 3D printing high-light-transmittance bio-ink, an artificial 3D algal-bacterial symbiont and a preparation method and application of the artificial 3D algal-bacterial symbiont. The 3D printing high-light-transmittance bio-ink is prepared from polyether F127 diacrylate, polyethylene glycol diacrylate, pyridine degradation special-effect bacterium paracoccus sp. NJUST47, chlorella sorokiniana FACHB-275, a light absorbent and a liquid culture medium containing a photoinitiator, and the 3D printing high-light-transmittance bio-ink is prepared into an artificial 3D bacterium-algae symbiont through one-step 3D printing. The 3D printing high-light-transmittance bio-ink has good synergistic pyridine degradation performance, the constructed artificial 3D algal-bacterial symbiont has high algal-bacterial loading capacity and excellent light utilization rate, enhanced removal of volatile toxic organic pollutant pyridine can be achieved, and degradation stability is good.

The invention discloses a bacterial co-culture metabolite for improving the yield of bacteriocin and application thereof, and is characterized in that the bacterial co-culture metabolite comprises at least one of L-chorolactone, N-acetyl aspartic acid, butyryl trihexyl citrate, N-acetyl-L-glutamic acid and ascorbic acid; the invention also provides an application of the bacterial co-culture metabolite in preparation of a fermentation medium for improving the yield of bacteriocin, and the fermentation medium is prepared by adding 0.3% by mass of L-chorolactone, 0.1% by mass of N-acetylaspartic acid, 0.1% by mass of butyryl trihexyl citrate, 0.1% by mass of L-aspartic acid, 0.1% by mass of N-acetylaspartic acid, 0.1% by mass of citric acid butyryl trihexyl ester, 0.1% by The culture medium is an MRS liquid culture medium containing 0.2% of N-acetyl-L-glutamic acid and 0.2% of ascorbic acid or an MRS liquid culture medium containing 0.1% of N-acetyl aspartic acid, 0.1% of L-chorolactone, 0.3% of ascorbic acid and 0.3% of butyryl trihexyl citrate in mass concentration. The culture medium has the advantages that the yield of bacteriocin of co-culture and single bacteria is increased.

The invention discloses a soil carbon sequestration microorganism isolated culture method under medium and high petroleum pollution stress and application, and the isolated culture method comprises the following steps: dispersing medium and high petroleum pollution soil surface soil in sterile water to obtain an original bacterial suspension containing carbon sequestration microorganisms, and carrying out gradient dilution on the original bacterial suspension; respectively coating on a carbon-source-free solid culture medium for culturing, purifying a bacterial colony obtained by culturing, and carrying out step-by-step amplification culture on a single bacterial colony obtained by purifying in a carbon-source-free liquid culture medium. The method has the beneficial effects that the invention provides the method for enriching and screening the indigenous indigenous efficient carbon sequestration strains with the carbon sequestration function from the medium-high petroleum pollution soil sample, and the four strains which can survive in the medium-high petroleum pollution stress environment and have good carbon dioxide sequestration capability are obtained through the method; the strains have potential pollution reduction and carbon sequestration synergistic capability, and a new technical path can be provided for green remediation and carbon sequestration of petroleum-contaminated soil.

The invention discloses a method for simultaneously extracting melanin and a bacterial extracellular vesicle compound from porphyromonas gingivalis and application of the method. The method comprises the following steps: inoculating a solid culture medium with a porphyromonas gingivalis seed solution, and carrying out anaerobic culture overnight at 37 DEG C; picking single colonies, putting the single colonies into a liquid culture medium, performing anaerobic culture at 37 DEG C for 24 hours, and then putting the single colonies into a bacterial incubator to be continuously cultured until OD600 is equal to 1; centrifugally separating supernatant liquid, and centrifuging again to obtain clarified supernatant liquid; and filtering the clarified supernatant liquid, carrying out ultrafiltration centrifugation on the obtained filtrate, washing a filter screen with water to obtain a washing liquid, carrying out ultrasonic treatment to obtain a melanin and bacterial exovesicle compound, carrying out vacuum freeze drying, and storing the melanin and bacterial exovesicle compound in a refrigerator. The method is economical, simple and convenient, the bacterial outer membrane vesicles and the melanin can be extracted at the same time, and the melanin and bacterial outer vesicle compound can achieve the effects of an immune agonist and a photo-thermal agent at the same time under 808 nm near-infrared laser irradiation.

The invention relates to bacillus velezensis CGMCC (China General Microbiological Culture Collection Center) No.32593 as well as preparation and application of a microbial agent of the bacillus velezensis, and belongs to the technical field of microorganisms. The strain disclosed by the invention has the effects of dissolving phosphorus, dissolving potassium, fixing nitrogen and producing indoleacetic acid (IAA) at the same time. The method comprises the following steps: inoculating bacillus velezensis CGMCC No.32593 into an LB liquid culture medium, and fermenting by using a 5L fermentation tank to obtain the liquid microbial agent. The microbial agent provided by the invention is simple in preparation method and convenient for industrial production. By applying the liquid microbial agent, the plant height of tomatoes and the fresh weight of overground parts can be effectively increased, and the utilization rate of soil phosphorus in crop production is increased.

The invention discloses a biological challenge device with combined adjustable resistance for evaluating the effect of a sterilization process, which comprises a shell, the shell comprises a plastic pipe and an inner pipe cap detachably connected with the inner wall of the plastic pipe, the inner pipe cap is provided with a breathable window and covered by a breathable film, and a bacterial contamination carrier for bearing indicator biological spores is attached to the inner bottom of the plastic pipe. An openable container containing a liquid culture medium is arranged in the plastic pipe cavity; the outer side of the inner pipe cap is sleeved with an outer pipe cap detachably connected with the outer wall of the plastic pipe, and the outer pipe cap is provided with a flow blocking structure used for adjusting the speed of the sterilizing agent entering the area of the inner pipe cap; the indicator biological spores are pretreated by a stable protective agent containing one or more of polyethylene glycol, trehalose, sorbitol, polyvinylpyrrolidone and glyceryl stearate. The speed of a sterilizing agent entering the device can be limited, the spores are dry in a suspension added with a stable protective agent, the capability of resisting general environmental changes is very good, and the performance of the device can be maintained for a long time.

The invention belongs to the technical field of medical preparations for pets, and particularly relates to an aloe extract-probiotic composite hydrogel for pet dermatosis, a preparation method comprises the following steps: under an aseptic condition, animal combined lactobacillus ZYpet-012 with the preservation number of CGMCC No.32008 is subjected to solid slant culture medium recovery and liquid culture, and then the animal combined lactobacillus ZYpet-012 with the preservation number of CGMCC No.32008 is subjected to freeze-drying to obtain the aloe extract-probiotic composite hydrogel for pet dermatosis. Centrifugally washing to obtain a probiotic suspension; the preparation method comprises the following steps: preparing blank hydrogel from carbomer 940, triethanolamine, zinc hyaluronate and the like; and mixing the aloe extract solution with the blank hydrogel, and then adding the probiotic suspension to prepare the composite hydrogel. According to the present invention, by using the anti-inflammatory, antibacterial and prebiotic characteristics of the aloe extract and combining the skin micro-ecology adjusting effect of the animal and the lactobacillus ZYpet-012, the prepared composite hydrogel can effectively treat the skin diseases of pets, and has advantages of nature, safety, accurate targeting and the like.

The invention belongs to the technical field of fungal fermentation, and particularly discloses ganoderma lucidum fermentation liquor and a preparation method thereof. The preparation method of the ganoderma lucidum fermentation liquor comprises the following steps: (1) activating a strain, namely inoculating the strain into a slant culture medium for culturing, and carrying out transfer grafting once to obtain an activated strain; (2) liquid culture: inoculating the activated strain into a liquid culture medium, and carrying out shake culture for 7 days to obtain a liquid strain; (3) fermentation culture: inoculating the liquid strain into a fermentation culture medium according to the inoculation amount of 7-9%, and carrying out three-stage culture to obtain a fermented product; and (4) centrifugal filtration: centrifuging the fermented product, collecting a supernatant, and filtering the obtained supernatant to obtain the ganoderma lucidum fermentation broth. According to the preparation method disclosed by the invention, three-stage variable-condition culture is specifically carried out according to the growth condition of the ganoderma lucidum mycelia, so that rapid growth of the ganoderma lucidum mycelia and mass accumulation of polysaccharides are facilitated, and the polysaccharide content of the prepared ganoderma lucidum fermentation liquor is increased.

The invention discloses a method for improving biocontrol photosynthetic bacteria through a far-source hybridization and genome rearrangement technology. The method comprises the following steps: inoculating a rhodopseudomonas palustris GJ-22 seed into a photosynthetic bacteria liquid culture medium for culturing; inoculating the bacillus subtilis KY-2 into an LB liquid culture medium for culturing; the cultured GJ-22 is taken and subjected to treatment and centrifugation, and then a GJ-22 protoplast is obtained; kY-2 is taken to be treated, and KY-2 protoplast is obtained after centrifugation; fusing the obtained GJ-22 protoplast and KY-2 protoplast, diluting a photosynthetic bacterium hypertonic liquid culture medium, and regenerating on a photosynthetic bacterium soft agar culture medium to obtain a fused strain; a fusion strain is screened out through an induced tobacco core leaf anti-TMV test to serve as a starting strain for genome rearrangement. Preparing protoplast from the original strain; dividing the prepared protoplast into two groups, and respectively carrying out ultraviolet inactivation and thermal inactivation; respectively fusing the inactivated protoplasts, and regenerating to obtain fusant strains; finally, a target strain with genetic stability is screened through a fusant strain induced tobacco core leaf anti-TMV test.

The invention belongs to the technical field of microorganisms, and particularly relates to application of utilizing alternaria alternata to ferment pericarpium citri reticulatae so as to improve the content of total flavonoids and hesperidin in pericarpium citri reticulatae, which comprises the following steps: culturing alternaria alternata spore suspension in a PDB liquid culture solution to obtain alternaria alternata inoculation liquid; uniformly mixing the sterilized pericarpium citri reticulatae powder and the sterilized rice powder, adding the alternaria alternata inoculation liquid, fully and uniformly mixing, and then adding sterilized water; after uniform stirring, the mixture is placed in a constant-temperature incubator to be fermented and co-cultured, the fermentation temperature is 25-35 DEG C, and the fermentation time is 5-12 days. According to the method disclosed by the invention, the efficient capability of the alternaria alternata in the aspect of converting flavonoid components in the pericarpium citri reticulatae can be fully exerted, so that the content of total flavonoids and hesperidin in the pericarpium citri reticulatae is remarkably increased.