656 results about "Liquid culture" patented technology

The invention relates to the field of piggery tail end odor pollution treatment, and discloses a piggery tail end odor purification filter element preparation method and application, the preparation method comprises the following steps: loading a photocatalyst on the surface of a filter element body to obtain a carrier, respectively inoculating 3-5 kinds of antagonism-free deodorization microorganisms into corresponding LB liquid culture mediums for activation and propagation, and carrying out purification on the LB liquid culture mediums to obtain the piggery tail end odor purification filter element. The preparation method comprises the following steps: preparing a bacterial solution, mixing the bacterial solution to obtain a corresponding bacterial solution, compounding all the bacterial solutions in proportion to obtain a compound bacterial solution, infiltrating a carrier in the compound bacterial solution, and carrying out biofilm formation at room temperature to obtain the filter element. After the photocatalyst on the surface is illuminated, some refractory pollutants can be decomposed into smaller intermediate products which are easier to utilize by microorganisms by utilizing light energy, a more suitable substrate is provided for the microorganisms, and the microorganisms can further thoroughly decompose the intermediate products into harmless substances such as carbon dioxide and water, so that the degradation effect is improved. Therefore, the overall degradation efficiency and degree of pollutants are improved.

The invention relates to the technical field of microorganisms and particularly discloses bacillus velezensis for producing tetramethylpyrazine and application of the bacillus velezensis, the preservation number of the strain is CCTCC NO: M20232058, and the bacillus velezensis is obtained by separating and purifying from high-temperature Daqu of Maotai-flavor liquor in the liquor industry of Jin-brand Maotai Town in Guizhou. The bacillus velezensis disclosed by the invention can produce 1875.4 mg / L of tetramethylpyrazine by performing shake flask fermentation on a liquid culture medium for 7 days, and the tetramethylpyrazine can reach 4810.8 mg / L by optimizing a culture medium formula and culture conditions. The bacillus velezensis is applied to small-scale brewing of Maotai-flavor liquor, the content of tetramethylpyrazine in the Maotai-flavor liquor reaches 34.5 + / -1.4 mg / L and is increased by 325.93% compared with a contrast, and the bacillus velezensis can be used for increasing the content of tetramethylpyrazine in the Maotai-flavor liquor.

A method for enhancing hydrogen uptake by cells of an acetogenic bacterium in a culture is provided. The method comprises incubating the cells in a liquid medium supplemented with an external gas mixture, wherein the external gas mixture comprises H2 and CO2; overexpressing an uptake hydrogenase and an associated electron transfer protein in the cells; increasing uptake of the H2 by the cells; and enhancing uptake of the CO2 by the cells. The culture may be a coculture with additional cells of a non-acetogenic bacterium, which produces natively H2 from catabolism of an organic molecule.

The invention discloses a fermentation method of recombinant bacillus subtilis for expressing vibrio parahaemolyticus outer membrane protein, which comprises the following steps: inoculating a recombinant bacillus subtilis strain into a liquid culture medium for activation and amplification to obtain a seed solution; transferring the seed solution into a fermentation tank according to the inoculum size of 5%-10%, controlling the temperature to be 35-37 DEG C and the pH to be 6.8-7.2, introducing sterile air and stirring, and monitoring the thallus concentration OD600 value in real time; when the OD600 value reaches 35-38, 10% of an inducer is added for pre-induction; when the OD600 value reaches 40-45, the remaining 90% of the inducer is added for main induction, and the real-time dissolved oxygen value is collected; when the real-time dissolved oxygen value is continuously higher than a first preset threshold value, the flow acceleration rate of the inducer is increased, the fermentation temperature is increased at the same time or step by step, and the increase amplitude of the dissolved oxygen value is in positive correlation with the flow acceleration rate and the temperature increase amplitude; when the OD600 value is stabilized in a preset interval, it is judged that fermentation is completed, and the recombinant bacillus subtilis thalli are obtained. The yield and quality of the vibrio parahaemolyticus outer membrane protein can be effectively improved.

The invention belongs to the technical field of preparation of probiotic products, and relates to a lactic acid bacteria microcapsule as well as a preparation method and application thereof. The method comprises the following steps: uniformly mixing sodium alginate, modified montmorillonite and sterile water, cooling, adding a prepared lactic acid bacteria seed solution, and fully and uniformly mixing to form an emulsion; spraying the emulsion into a calcium chloride solution by adopting an extrusion method, stirring and curing to obtain microcapsules; and culturing the prepared microcapsule in a lactic acid bacteria liquid culture medium to obtain the lactic acid bacteria microcapsule after the culture is finished. The lactobacillus is effectively protected by adopting an extrusion-method single coating and post-coating culture process, so that the stability of the lactobacillus to a humid and hot environment and the gastrointestinal stress resistance are improved, and meanwhile, the release performance of the lactobacillus in the gastrointestinal tract is ensured. The problem that an existing microcapsule coating technology cannot achieve balance between improvement of the hygrothermal stability and gastrointestinal stress resistance of the probiotics and guarantee of good release performance of the probiotics in the gastrointestinal tract is solved.

The invention relates to a method for producing notoginsenoside and ginsenoside by utilizing panax notoginseng cotyledon embryos, and belongs to the technical field of plant adventitious root tissue culture. The method comprises the following steps: inducing embryogenic calluses by utilizing immature zygotic embryos of panax notoginseng; proliferating the panax notoginseng embryogenic callus on a plate culture medium; s2, putting the pseudo-ginseng embryogenic callus obtained in the step S2 into a liquid culture medium, carrying out cell dispersion, and sieving by using a sieve with the aperture of 24-30 meshes; a mechanical stirring type bioreactor is filled with a liquid culture medium, and the globular embryo and the heart-shaped embryo are placed in the mechanical stirring type bioreactor to be cultured; s3, recovering the filtrate in the step S3, filtering by using a screen with the aperture of 30-80 meshes and 80-150 meshes to obtain uniform panax notoginseng embryogenic cells, and differentiating the panax notoginseng embryogenic cells in a triangular flask for the step S4; and drying the cotyledon embryo obtained in the step S4 at 45 DEG C, and grinding into powder. According to the method, large-scale generation of cotyledon embryos can be completed only in 4-6 weeks, the production process is remarkably accelerated, the method is suitable for industrial batch production requirements, and the production efficiency is high.

The invention provides a culture method for increasing the content of a bioactive substance Emodic acid in talaromyces purpurascens, and relates to the technical field of medicinal fungus culture. The culture method of the homopurple talaromyces spp. Comprises the following steps: carrying out liquid culture on hyphae of the homopurple talaromyces spp., and then inoculating the hyphae into a liquid culture medium containing black bean powder for culture. The defects in the prior art are overcome, and the pharmacological activity of the talaromyces purpurascens is enhanced by optimizing the formula of the fermentation culture medium and improving the fermentation conditions, so that the content of Emodic acid in the talaromyces purpurascens is increased.

The embodiment of the invention provides a method for converting ginsenoside in ginseng adventitious roots, which comprises the following steps: (a) taking liquid-cultured ginseng adventitious roots as raw materials, taking the ginseng adventitious roots out of a liquid culture medium, cleaning and removing the residual liquid culture medium on the surfaces of the ginseng adventitious roots, and removing moisture on the surfaces of the ginseng adventitious roots; and (b) suspending the ginseng adventitious roots obtained in the step (a) in high-temperature and high-pressure equipment, adding water into the bottom of the high-temperature and high-pressure equipment, heating to generate high-temperature and high-pressure steam to carry out high-temperature and high-pressure conversion on the ginseng adventitious roots, and controlling reaction parameters to obtain finished ginseng adventitious roots with the rare ginsenoside content obviously improved.

The invention belongs to the technical field of microorganisms and fermentation engineering, and particularly relates to application of bifidobacterium longum subsp. Infantis Y46 in the aspects of enhancing cell defense and repair and regulating metabolic homeostasis of a host. The strain is isolated from infant feces. Animal experiments prove that living cell thalli of the bifidobacterium longum subsp. Infantis Y46, which are obtained by culturing an MRS liquid culture medium added with L-cysteine hydrochloride (0.05%, v / v), can significantly enhance the oxidative stress resistance of an organism and reduce the level of active oxygen in the organism compared with a control bacterium bifidobacterium longum subsp. Infantis 15697. Transcriptomics analysis shows that the bifidobacterium longum subsp. Infantis Y46 can enhance self-protection of cells and improve physiological imbalance caused by external pressure by regulating related signal channels of an organism. The action mechanism is closely related to signaling pathways related to cell defense, metabolic homeostasis and barrier functions. The invention provides a new strain resource and an application scheme for developing functional food or medicinal preparations for enhancing the health defense function of a human body.

The invention provides a culture method for increasing the content of antroquinonol in antrodia camphorata mycelia, and relates to the technical field of medicinal fungus culture. The culture method comprises the steps that antrodia camphorata mycelia are inoculated into a liquid culture medium added with meliaceae cedrela sinensis tender leaf homogenate to be cultured, the liquid culture medium is a PDA culture medium, and the adding amount of the meliaceae cedrela sinensis tender leaf homogenate is 0.5-2 g / 100 mL. According to the method, the defects in the prior art are overcome, the content of antroquinonol in the antrodia camphorata mycelia is increased by optimizing the formula of the fermentation culture medium and improving the fermentation conditions, and the pharmacological activity of the antrodia camphorata mycelia is enhanced.

The invention belongs to the technical field of microorganisms and fermentation engineering, and particularly relates to application of bifidobacterium longum subsp. Infantis Y46 in the anti-aging aspect. The bifidobacterium longum subsp. Infantis Y46 is obtained from excrement of infants. Animal experiments prove that living cell thalli (Y46) and thermally inactivated cell thalli (HKY46) obtained by culturing the bifidobacterium longum subsp. Infantis Y46 by using an MRS liquid culture medium containing L-cysteine hydrochloride (0.05%, v / v) can significantly reduce accumulation of lipofuscin in a host, and prolong the healthy life and life of the host. Therefore, the bifidobacterium longum subsp. Infantis Y46 is a promising probiotic and a promising metagen with an anti-aging function.

The invention belongs to the technical field of genetic engineering of edible mushrooms, and particularly relates to a construction method of an efficient and stable genetic transformation system of flammulina velutipes. The construction method comprises the following steps: culturing in an MM culture medium by taking a flammulina velutipes strain 0747 as a receptor; the pFgnpt vector is converted into agrobacterium AGL1 (AGL1); inoculating hyphae into an IM liquid culture medium containing acetosyringone and MES, mixing with agrobacterium tumefaciens, culturing, and transferring the hyphae to an IM solid flat plate paved with filter paper; hyphae are transferred to a PDA resistance plate containing G418 and cephalosporin for resistance screening, and an efficient and stable flammulina velutipes genetic transformation system is obtained. The new selection marker G418 is developed and applied, limitation of a traditional selection marker is broken, more choices are provided for flammulina velutipes genetic transformation, a new thought is provided for genetic transformation research of other edible mushrooms, and the application field of an agrobacterium tumefaciens-mediated transformation technology is expanded.

The invention discloses 3D printing high-light-transmittance bio-ink, an artificial 3D algal-bacterial symbiont and a preparation method and application of the artificial 3D algal-bacterial symbiont. The 3D printing high-light-transmittance bio-ink is prepared from polyether F127 diacrylate, polyethylene glycol diacrylate, pyridine degradation special-effect bacterium paracoccus sp. NJUST47, chlorella sorokiniana FACHB-275, a light absorbent and a liquid culture medium containing a photoinitiator, and the 3D printing high-light-transmittance bio-ink is prepared into an artificial 3D bacterium-algae symbiont through one-step 3D printing. The 3D printing high-light-transmittance bio-ink has good synergistic pyridine degradation performance, the constructed artificial 3D algal-bacterial symbiont has high algal-bacterial loading capacity and excellent light utilization rate, enhanced removal of volatile toxic organic pollutant pyridine can be achieved, and degradation stability is good.

The invention discloses a bacterial co-culture metabolite for improving the yield of bacteriocin and application thereof, and is characterized in that the bacterial co-culture metabolite comprises at least one of L-chorolactone, N-acetyl aspartic acid, butyryl trihexyl citrate, N-acetyl-L-glutamic acid and ascorbic acid; the invention also provides an application of the bacterial co-culture metabolite in preparation of a fermentation medium for improving the yield of bacteriocin, and the fermentation medium is prepared by adding 0.3% by mass of L-chorolactone, 0.1% by mass of N-acetylaspartic acid, 0.1% by mass of butyryl trihexyl citrate, 0.1% by mass of L-aspartic acid, 0.1% by mass of N-acetylaspartic acid, 0.1% by mass of citric acid butyryl trihexyl ester, 0.1% by The culture medium is an MRS liquid culture medium containing 0.2% of N-acetyl-L-glutamic acid and 0.2% of ascorbic acid or an MRS liquid culture medium containing 0.1% of N-acetyl aspartic acid, 0.1% of L-chorolactone, 0.3% of ascorbic acid and 0.3% of butyryl trihexyl citrate in mass concentration. The culture medium has the advantages that the yield of bacteriocin of co-culture and single bacteria is increased.

The invention relates to bacillus velezensis CGMCC (China General Microbiological Culture Collection Center) No.32593 as well as preparation and application of a microbial agent of the bacillus velezensis, and belongs to the technical field of microorganisms. The strain disclosed by the invention has the effects of dissolving phosphorus, dissolving potassium, fixing nitrogen and producing indoleacetic acid (IAA) at the same time. The method comprises the following steps: inoculating bacillus velezensis CGMCC No.32593 into an LB liquid culture medium, and fermenting by using a 5L fermentation tank to obtain the liquid microbial agent. The microbial agent provided by the invention is simple in preparation method and convenient for industrial production. By applying the liquid microbial agent, the plant height of tomatoes and the fresh weight of overground parts can be effectively increased, and the utilization rate of soil phosphorus in crop production is increased.

The invention discloses a biological challenge device with combined adjustable resistance for evaluating the effect of a sterilization process, which comprises a shell, the shell comprises a plastic pipe and an inner pipe cap detachably connected with the inner wall of the plastic pipe, the inner pipe cap is provided with a breathable window and covered by a breathable film, and a bacterial contamination carrier for bearing indicator biological spores is attached to the inner bottom of the plastic pipe. An openable container containing a liquid culture medium is arranged in the plastic pipe cavity; the outer side of the inner pipe cap is sleeved with an outer pipe cap detachably connected with the outer wall of the plastic pipe, and the outer pipe cap is provided with a flow blocking structure used for adjusting the speed of the sterilizing agent entering the area of the inner pipe cap; the indicator biological spores are pretreated by a stable protective agent containing one or more of polyethylene glycol, trehalose, sorbitol, polyvinylpyrrolidone and glyceryl stearate. The speed of a sterilizing agent entering the device can be limited, the spores are dry in a suspension added with a stable protective agent, the capability of resisting general environmental changes is very good, and the performance of the device can be maintained for a long time.

The invention belongs to the technical field of medical preparations for pets, and particularly relates to an aloe extract-probiotic composite hydrogel for pet dermatosis, a preparation method comprises the following steps: under an aseptic condition, animal combined lactobacillus ZYpet-012 with the preservation number of CGMCC No.32008 is subjected to solid slant culture medium recovery and liquid culture, and then the animal combined lactobacillus ZYpet-012 with the preservation number of CGMCC No.32008 is subjected to freeze-drying to obtain the aloe extract-probiotic composite hydrogel for pet dermatosis. Centrifugally washing to obtain a probiotic suspension; the preparation method comprises the following steps: preparing blank hydrogel from carbomer 940, triethanolamine, zinc hyaluronate and the like; and mixing the aloe extract solution with the blank hydrogel, and then adding the probiotic suspension to prepare the composite hydrogel. According to the present invention, by using the anti-inflammatory, antibacterial and prebiotic characteristics of the aloe extract and combining the skin micro-ecology adjusting effect of the animal and the lactobacillus ZYpet-012, the prepared composite hydrogel can effectively treat the skin diseases of pets, and has advantages of nature, safety, accurate targeting and the like.

The invention belongs to the technical field of fungal fermentation, and particularly discloses ganoderma lucidum fermentation liquor and a preparation method thereof. The preparation method of the ganoderma lucidum fermentation liquor comprises the following steps: (1) activating a strain, namely inoculating the strain into a slant culture medium for culturing, and carrying out transfer grafting once to obtain an activated strain; (2) liquid culture: inoculating the activated strain into a liquid culture medium, and carrying out shake culture for 7 days to obtain a liquid strain; (3) fermentation culture: inoculating the liquid strain into a fermentation culture medium according to the inoculation amount of 7-9%, and carrying out three-stage culture to obtain a fermented product; and (4) centrifugal filtration: centrifuging the fermented product, collecting a supernatant, and filtering the obtained supernatant to obtain the ganoderma lucidum fermentation broth. According to the preparation method disclosed by the invention, three-stage variable-condition culture is specifically carried out according to the growth condition of the ganoderma lucidum mycelia, so that rapid growth of the ganoderma lucidum mycelia and mass accumulation of polysaccharides are facilitated, and the polysaccharide content of the prepared ganoderma lucidum fermentation liquor is increased.

The invention discloses a method for quickly and efficiently differentiating iPSC (induced pluripotent stem cells) into mesenchymal stem cells in a serum-free manner, the method comprises the following steps: firstly inducing the iPSC into a mesoderm precursor, then transferring the mesoderm precursor into a liquid culture medium for suspension culture, and finally obtaining iMSC (iPSC-doped MSC) by using an enrichment culture medium, and the method can shorten the differentiation time, improve the purity of the iMSC and obtain a large number of homogenized iMSCs.

The invention discloses a high-yield flower and fruit aroma strain, culture thereof and application of the high-yield flower and fruit aroma strain in sauce-flavor food brewing, the strain is named as Wickerhamomyces anomalus GZZJ2023Y053, and is preserved in the China Center for Type Culture Collection (CCTCC) with the preservation number of CCTCC NO: M 2025875, and the preservation number of the strain is CCTCC NO: M 2025875. The preservation date is April 24, 2025; the preservation address is Wuhan University, Wuhan, China. The culture method of Wickerhamomyces anomalus comprises the following steps: inoculating activated Wickerhamomyces anomalus GZZJ2023Y053 into a liquid culture medium, and fermenting at constant temperature to obtain a seed solution; the invention also provides an application of the Wickerhamomyces anomalus GZZJ2023Y053 in preparation of ethyl acetate and brewing of sauce-flavor foods. According to the method, multiple Wickerhamomyces anomalus separated from fermented grains is subjected to separation culture, the content of ethyl acetate is measured after fermentation is finished, then the screened Wickerhamomyces anomalus GZZJ2023Y053 has the capacity of producing ethyl acetate at high yield, and after fermentation conditions are further optimized, the yield of the ethyl acetate is improved. The capability of synthesizing ethyl acetate is greatly improved.

The invention discloses an expression system suitable for short-chain non-specific peroxidase and application of the expression system, and belongs to the technical field of enzyme engineering and genetic engineering. According to the expression system disclosed by the invention, a nucleotide sequence of short-chain non-specific peroxidase with SUMO and 8HIS tags is connected to a pET28a vector to obtain a recombinant vector, and escherichia coli BL21 (DE3) is transformed to obtain a genetically engineered bacterium; genetically engineered bacteria are subjected to two-stage temperature control culture in a ZYM5052 liquid culture medium, and meanwhile, 5ALA is added into the culture medium. According to the invention, an escherichia coli self-induction system is used, so that a shake-flask culture can grow at high density in a short time, the culture medium contains rich nutrient substances, and monitoring of cell density and adding of IPTG are not needed in the expression process. Meanwhile, a heme precursor 5ALA is added into an escherichia coli self-induction culture medium, and finally the high-activity short-chain non-specific peroxidase is obtained.

The invention provides a fermentation method for producing single-cell protein from corn straw. The fermentation method comprises the following steps: (1) inoculating a microbial seed solution into a crushed corn straw solid culture medium; (2) after the solid-state fermentation is completed, adding water, and reacting at 65-85 DEG C for 4-8 hours; and (3) cooling the liquid culture medium, and then inoculating a microbial seed solution for fermentation. By adopting the method, the yield of single-cell protein can be up to 142.68 g / L, and the yield of crude protein can be up to 44.8%. Compared with direct fermentation of the corn straw, the yield of single-cell protein and the content of crude protein can be remarkably increased, and the fermentation method for achieving efficient utilization of the corn straw is provided.

The invention discloses a long lysinibacillus inoculant, a preparation method and application, the inoculant is prepared from a long lysinibacillus strain C1, and the preparation method comprises the following steps: culturing the strain of the long lysinibacillus strain through a tryptone soybean agar culture medium until yellow single colonies appear; inoculating the yellow single colony into serum, and carrying out seed culture by using a long lysine bacillus liquid culture medium until a logarithmic phase to obtain a bacterial solution; and inoculating the cultured bacterial liquid into a production device according to a long lysine bacillus strain, and carrying out production culture by using a long lysine bacillus production culture medium to a logarithmic phase to prepare the long lysine bacillus biocontrol inoculant. And carrying out centrifugal treatment on the obtained biocontrol microbial inoculum of the long lysine bacillus, taking a supernatant, and filtering the supernatant to obtain the long lysine bacillus biocontrol fermentation broth. The invention further discloses application of the lysinibacillus elongate microbial inoculum in antagonism of rice blast bacteria and prevention and control of rice blast.

The utility model belongs to the technical field of plant tissue culture devices, and particularly relates to a plant tissue culture device which comprises a tray, a plurality of plant tissue culture devices and a plurality of plant tissue culture devices, the tray comprises a supporting frame and a hole tray, and the hole tray is placed on the supporting frame and provided with a plurality of circular hole grooves; the tissue culture bag is placed in the hole groove and comprises a bag body, the section of the bag body is circular, the diameter of the bag body is gradually increased from bottom to top, a hard ring is arranged at the top of the bag body, a groove is formed in the bottom of the hard ring, at least three hanging lugs are arranged on the outer wall of the hard ring, and air holes are formed in the bag body; provided is a tissue culture frame. The tissue culture bag is small in bottom area and large in top opening area, and the inoculation area is increased under the condition that the same amount of culture medium is injected; the circular bottom design ensures that the culture medium can be upright without falling after being solidified; the cylindrical bag body is designed, so that the tissue culture bag is not easy to collapse; the tissue culture frame can maintain the shape of the tissue culture bag body not to be deformed, and a liquid culture medium can be conveniently and rapidly canned in production.

The utility model relates to a tremella liquid strain inoculation device which comprises a rack, a conveying roller mechanism used for transversely conveying a culture bottle tray is arranged on the rack, a first station and a second station are sequentially arranged on the conveying roller mechanism in the conveying direction, a first blocking mechanism used for blocking the tray is arranged on the first station, and a second blocking mechanism used for blocking the tray is arranged on the second station. A drilling mechanism which can ascend and descend and is used for enabling a culture medium in the culture bottle to form a concave hole is arranged above the first station, a second blocking mechanism which is used for blocking the tray is arranged on the second station, and a bacterium injection mechanism which can ascend and descend and is used for injecting a liquid strain into the concave hole of the culture medium is arranged above the second station. The device is compact in structure, inoculation of liquid strains in the tremella culture bottle can be conveniently achieved, and the liquid strains can enter a culture medium more quickly.

Device (1) for the application of at least one liquid medium and method for the application of at least one liquid medium are proposed, wherein, due to the design, a soil-friendly application of at least one liquid medium is ensured.

The invention provides a soil remediation method for jointly degrading polycyclic aromatic hydrocarbon by zoysia sinensis-degrading flora, which comprises the following steps: S1, carrying out shake culture on a soil sample in a shaking table, and then culturing in a liquid culture medium to obtain an enriched bacteria solution; s2, adding PAHs into a liquid culture medium, inoculating the enriched bacterial liquid into the liquid culture medium, and gradually increasing the concentration of the PAHs to obtain a culture solution; s3, centrifuging the culture solution, collecting thalli, performing enrichment culture again, adding the bacterial solution into glycerol, and mixing to obtain a degrading flora bacterial solution; s4, mixing the polycyclic aromatic hydrocarbon contaminated soil with nutrient soil to obtain composite soil; s5, sowing the zoysia sinensis seeds in the composite soil to obtain plants, and culturing the plants in a plant culture room; and step S6, adding activated degrading flora bacterial liquid into the plants on the 15th day and the 30th day after sowing. Through the combined degradation effect of plants and microorganisms, the content of PAHs in soil is reduced.

The invention provides a grifola frondosa liquid culture medium as well as a culture method and application thereof, and relates to the technical field of fermentation. The invention provides a grifola frondosa liquid culture medium, which contains glucose, peptone, KH2P2O4, MgSO4, vitamin B1, gibberellin and tyrosol, can significantly improve the growth of grifola frondosa thalli and the secretion of exopolysaccharides, and can be used for the preparation of food, drugs and health care products.

The invention discloses a marine fungus fusarium keratinoides and application thereof. The fusarium keratinoides capable of efficiently producing the perindoprine is screened from marine sediments, and the content of the produced perindoprine can reach 561.7 mu g / L after the fusarium keratinoides is fermented in a GPY liquid culture medium, so that possibility and a foundation are provided for breeding strains suitable for industrial large-scale production of the perindoprine in the future; mass spectrum, hydrogen spectrum and carbon spectrum data of the separated perindoprine are completely consistent with perindoprine data reported in literatures.

The invention discloses a liquid strain culture device and culture method for black skin termitomyces albuminosus, and relates to the technical field of strain culture, the liquid strain culture device comprises a supporting assembly, a light reflecting assembly and a liquid supplementing assembly, the supporting assembly comprises four supporting columns, the outer surface of one supporting column is fixedly connected with a mounting plate, and the outer surface of the other supporting column is fixedly connected with a fixing plate; and a controller and an electronic timer are arranged on the outer surface of the mounting plate. The illumination mode of light supplement is adjusted through monitoring of an electronic timer, meanwhile, an illumination sensor detects the illumination degree received by liquid strains in each area, an electric push rod is controlled to be started through a controller, the electric push rod is made to stretch out and draw back, the angle of a light reflecting plate is adjusted, and the light supplementing effect is improved. Therefore, the light reflecting surface can reflect outdoor light or outdoor lamplight to the liquid strain culture area to supplement light for the liquid strain culture area.