3666 results about "Liquid culture" patented technology

A photobioreactor includes a cultivation zone configured to contain a liquid culture medium and facilitate growth of a microalgae biomass, a plurality of parallel edge-lit light transmitting devices mounted within the cultivation zone, and a collection zone oriented in relation to the cultivation zone such that at least a portion of the liquid culture medium and microalgae from the cultivation zone may be periodically harvested. Methods for illuminating algae, for dissolving materials into an algae medium, for extracting oil from algae, and for producing biodiesel from algal oil are also provided.

The present invention provides a novel method for culturing cells as well as a novel method for producing a recombinant protein by culturing cells at large scale (up to 1,500 L nominal volume and 750 L working volume), whereby an inflated bag provides a sterile, disposable cultivation chamber. The inflated bag is partially filled with liquid cultivation media and cells, and placed into a containment vessel. The containment vessel is positioned onto an orbitally shaken platform. The orbital shaking moves the containment vessel and thus the bag and induces thereby motion to the liquid contained therein (“shake mixing”). This motion (caused by orbital shaking) induces a dynamic force field that ensures cell suspension, bulk mixing, and oxygen transfer from the liquid surface to the respiring cells without damaging shear or foam generation.

A method for cultivating Antrodia Camphorata is provided. First, Antrodia Camphorata is pre-incubated on a sloped surface culture medium. A portion of the pre-incubated Antrodia Camphorata is transferred to a liquid culture medium for incubation. Then, Antrodia Camphorata incubated in the liquid culture medium is moved to a solid culture medium for incubation. The cultivated Antrodia Camphorata has similar efficacies for inhibiting cancel cell growth, reducing the occurrence of atherosclerosis or recovering the damaged liver functions, as the wild Antrodia Camphorata.

A radiation sensing method and device that is used to measure physical properties of materials over a wide dynamic range. The sensor (20) comprises multiple radiation sources and multiple detectors at multiple separation distances. The detected signals from the different sources are separated and then combined mathematically in a manner such that the combination is self-compensated for both component drift and changes in radiation coupling efficiency between the source or detector and the material of interest. In a preferred embodiment, the biomass in a liquid cell culture (54) is measured with high accuracy over a wide dynamic range using optical wavelength radiation. The measurement can be made with the sensor external to the liquid culture container in a manner that is compensated for the thickness of the container window (50).

The invention discloses a production method of a pig feed additive containing traditional Chinese medicine and active probiotics, which comprises the steps of: respectively conducting single-spawn strain quantity expanding culture to the parent strains of active probiotics for pigs; mixing equiponderant first-order seed liquid of all kinds of active probiotics for pigs, inoculating the liquid into seed tank culture mediums for multi-spawn mixed culture and then inoculating the liquid into traditional Chinese medicine liquid culture mediums for primary traditional Chinese medicine fermentation; and mixing multi-spawn mixed traditional Chinese medicine fermentation liquid with corn cob meal with the same volume to obtain solid fermented seeds and then inoculating the seeds into traditional Chinese medicine solid culture mediums for secondary traditional Chinese medicine fermentation. By implementing the embodiment of the invention, the strain activity of the active probiotics in the pigfeed additive and the colonization performance in intestinal canals can be greatly improved, the produced pig feed additive is rich in traditional Chinese medicine compositions, the effect of active compositions which take a pharmacological effect in the traditional Chinese medicine compositions can be fully exerted, the body strengthening and disease curing performance and the growth promoting performance of the pig feed additive are improved and therefore the use of antibiotics can be fully avoided.

The invention relates to a method for preparing a nano-cellulose antibacterial composite material through on-line culture. The method comprises the following steps of: (1) inoculating an activated bacterial cellulose producing strain into a liquid culture medium, performing amplification culture, transferring the liquid culture medium into a bioreactor, culturing, adding an antibacterial material into the liquid culture medium, and continuing to culture to obtain an unpurified antibacterial bacterial cellulose composite material; and (2) peeling a bacterial cellulose membrane from a framework or directly soaking the composite material in a NaOH solution, treating in water bath, and washing until an obtained product is neutral to obtain the nano-cellulose antibacterial composite material. The preparation efficiency is high, and the method is simple, convenient, feasible and low in cost; and the surface of the bacterial cellulose composite material has a nanoscale three-dimensional fibrous mesh-shaped structure, the tensile strength of the material is greatly improved compared with that of a pure bacterial cellulose membrane, and the material can be widely applied to products such as facial masks, wound dressings, plasters, artificial skins and the like.

The invention relates to a bacterial cellulose/polymer composite film and a preparation method thereof. The bacterial cellulose/polymer composite film is formed by compounding bacterial cellulose fibers on a polymer porous material in situ. The method comprises the following steps of: (1) activating strains, finally inoculating into a liquid culture medium to culture, infiltrating the surface of the porous polymer film in the liquid culture medium and exposing in air; (2) repeating the operation every 2-3 days until a composite film is formed; (3) post-treating the composite film to be neutral to obtain a bacterial cellulose/polymer composite gel film; and (4) drying the gel film obtained in the step (3) to constant weight to obtain the bacterial cellulose/polymer composite dry film. The invention solves the problem of low mechanical strength of a single bacterial cellulose film. By utilizing hydrogen bonding produced by hydroxyl groups on cellulose macromolecules, the strength, the durability and the water absorbability of the bacterial cellulose/polymer composite gel film and the composite dry film are improved.

The invention discloses a preparing method of new type lipopeptide biosurfactant surfactin and appliance, which comprises the following steps: utilizing bacillus subtilis strain E8 (conserved in Chinese microbe conservative council common microbe center, the keeping number at 1107) to proceed microbe liquid ferment prepare surfactant surfactin; proceeding carbon source, nitrogen source and inorganic salt optimize for the needed liquid culture medium; getting fermentation liquor; centrifuging to remove thallus; acidifying supernatant; extracting rough extract; further-extracting through dissolvent; acidifying; collecting deposition; centrifuging; vacuum-drying; getting pre-refined product; freeze-drying through drainage pillar and FPLC extraction; getting product with high purity; making the output of acidifying extraction at 24-56 h reach 5-13g/L. The dominant fermentate molecular weight of high-yield strain E8 can reach 1022Da and CMC can reach 1 mu M, which is the lowest of reported surfactin CMC value.

A solid state fermentation (SSF) method is provided which is effective for both small- and large-scale fungal cultivation. Also provided is SSF media for fungal cultivation. The media preferably contains a carbon source and nitrogen source to provide a carbon to nitrogen ratio of about 5:1 to about 25:1 by weight. The media may also contain a vitamin and an inorganic substance. A preferred SSF medium contains malt extract, yeast extract, peptone, glucose, water, solid base, and calcium carbonate or gypsum. Before propagating fungal mycelia in the SSF medium, the mycelia may be pre-cultivated in a solid culture medium and then in a liquid medium. Although the SSF method can be used in growing most fungi, preferred fungi include Cordyceps sinensis, Ganoderma lucidum, Antrodia camphorata, Trametes versicolor, and Agaricus blazei. The SSF method not only produces high yield of fungi, but also stimulates the production of fungal metabolites, particularly the kinds with pharmaceutical and medicinal activities. Cordyceps sinensis is preferably grown to produce the active compound H1A which is a derivative of ergosterol.

The present invention relates to a method of producing biosurfactants, such as surfactin, comprising culturing at least one biosurfactant-producing microbe in a liquid culture medium comprising vinasse as a carbon source. Methods of using the crude biosurfactant containing culture broth in tertiary oil recovery and as antibacterial compositions in tertiary oil recovery are also described. Methods of using the culture broth residue after isolation of the biosurfactants as fertilizer and compositions for this use are also described.

The invention relates to a device and method for preparing a hollow heteromorphic bacteria cellulose material. The device comprises an airtight container, a second tubular component, a first tubular component and a cylindrical hollow plug, wherein the first tubular component and the second tubular component are coaxially sheathed inside the lumen of the second tubular component; an opening of the second tubular component is sealed with the first tubular component by the cylindrical hollow plug; and the gap between the first tubular component and the second tubular component is a space for storing fermentation medium. The method comprises the following steps: after inoculating fluid nutrient medium with bacterial cellulose-producing strain for spread cultivation, transferring the bacterial cellulose-producing strain to a fermentation device with an oxygen permeability mould, and culturing to obtain the heteromorphic bacteria cellulose material. The hollow heteromorphic bacteria cellulose (BC) material prepared by the invention not only has controllable size and shape, but also has the detachable and reused mould of the fermentation device, can be widely applied to substitute goods of hollow organs, such as artificial vascular grafts, nerve fiber conduits and the like, and can be served as the material for packing the foods, such as casings for meat products, jelly coatings and the like.

An apparatus and method of stirring cells in liquid culture media is provided. Baffles are mounted along the interior wall of the container and a hump is positioned on the center of the bottom surface of the container. A stirring apparatus is positioned inside the container. The blades of the stirring apparatus are angled outward toward the bottom of the container. The bottom edge of the blades extend closer to the bottom surface of the container then the apex of the hump. The liquid in the container is at a level below the top of the blades such that the blades are moved through the surface of the liquid. A magnetic bar is attached to the shaft and is driven by an external rotating magnetic bar.

The invention provides an acid-resistant bifidobacterium breve BB8dpH and an application thereof. The nucleotide sequence of the 16SrRNA gene part of the bifidobacterium breve BB8dpH is shown in SEQ ID NO.1, and is stored with the preservation number of CGMCCNo.8370. The bifidobacterium breve BB8dpH is a strain obtained by separating from the dejecta of healthy young people and further screening under the pH 3.2 acid condition. According to the fermentation and culturing process, anaerobic culture at 37 DEG C is carried out in a BL liquid substrate (containing 0.05% cysteine hydrochloride) for 24 hours. The bifidobacterium breve BB8dpH has remarkably better acid resistance than the common strains, and the acid resistance has genetic stability; the bifidobacterium breve BB8dpH has different percent contents of cell membrane fatty acids from the common strains, the average carbon chain length of the bifidobacterium breve BB8dpH is remarkably longer than that of the common strains, and the cell membrane fluidity of the bifidobacterium breve BB8dpH is remarkably lower than that of the common strains; and the bifidobacterium breve BB8dpH is used in the production field of daily fermented food, health-care food and medicines.

The invention discloses a preparation method for a bacterial cellulose composite membrane and application of the bacterial cellulose composite membrane as a facial mask material. The preparation method comprises the following steps: (1) selecting a strain; (2) preparing a seed containing a polyglutamic acid (PGA) and a liquid culture medium; (3) culturing seed cells; (4) producing bacterial cellulose by static liquid fermentation; and (5) purifying a bacterial cellulose membrane. The bacterial cellulose composite membrane can be used as a facial mask carrier or is directly used as a wet facial mask. The preparation method improves the yield of the bacterial cellulose by adding the polyglutamic acid (PGA) into the culture medium, improves the water content and the mechanical strength of a wet membrane, and has simple process and low cost; and the experiments prove that the method can ensure that the dry weight of the bacterial cellulose is improved to between 14 and 16g/L from the prior dry weight of between 8 and 10g/L, and the water content is increased to 99 percent from 98 percent.

The invention discloses a method for preparing a bioactive filler through immobilization of an anaerobic ammonia oxidizing bacterium, and belongs to the field of water treatment. The active filler is prepared by the following steps of: grinding active carbon, soaking in an anaerobic ammonia oxidizing bacterium liquid culture medium, and preparing an embedding solution from polyvinyl alcohol, calcium carbonate and condensed anaerobic ammonia oxidizing bacterium liquor in proportion; mixing and uniformly stirring the prepared embedding solution with soaked granular active carbon, and implementing immobilization through boric acid to finish cross-linking immobilization, thereby obtaining the active filler. The bioactive filler, after being cultured for 2 weeks, can recover anaerobic ammonia oxidizing activity. The bioactive filler disclosed by the invention is good in settlement performance and high in mechanical strength, and can achieve a micro-expansive state in an upflow biofilter.

The invention relates to a method for culturing microalgae by an illumination way and a reactor thereof, which belong to the technical field of biology. In the invention, carbon dioxide is injected into a pipeline through a carbon dioxide feeding device during the microalgae culturing process, and microalgae liquid culture from a fermentation tank and the injected carbon dioxide are fully mixed through a circulating pump and then flow in the pipeline; as the pipeline is twisted on a cylindrical light source, the carbon dioxide can be fully utilized for photosynthesize; and the microalgae culture that flows out from the pipeline enters the fermentation tank, then is fully mixed with aerated air by stirring of a stirring vane, and then pumped into the pipeline again to form flow circulation. The bioreactor adopting the method can absorb 40-80 percent of carbon dioxide in fixed aerated air under the condition of injecting 1-40 percent of carbon dioxide, obtains the microalgae with dry cell weight concentration up to more than 3.5g/L after five-day culture and achieves the purposes of effectively absorbing carbon dioxide and culturing the microalgae.

The invention discloses a method for culturing edible fungi, which includes the following steps: culturing the mother culture in larger scale to prepare liquid culture spawn, introducing the liquid culture spawn together with culture medium which needs to be sterilized separately to cultivating material for thick solid ventilated culturing, when the cultivating material is filled with hypha, briquetting and shaping the cultivating material, and sending the briquetted cultivating material to a mushroom house to produce mushroom. The method is environment-friendly, energy-saving, short in production period, low in pollution rate and small in work intensity.

The invention relates to a comprehensive factory-like circulation production method of pleurotus eryngii, and relates to the technical field of fungus plantation. By utilizing liquid cultures, the production efficiency of the pleurotus eryngii and the little oyster mushroom, pleurotus cornucopiae or pleurotus geesteranus is greatly improved, the amount of used labor and floor space are reduced, and cost is reduced. Due to the facts that local cotton stalks are utilized for producing the pleurotus eryngii, and a large number of waste mushroom dregs produced after the pleurotus eryngii is produced serve as main cultivating materials of little oyster mushroom, pleurotus cornucopiae or pleurotus geesteranus, cost is greatly reduced, and environmental pollution is removed. A semi-underground mushroom house is adopted for full-year little oyster mushroom, pleurotus cornucopiae or pleurotus geesteranus industrialized production, the limited problem that a traditional greenhouse is affected by seasons is solved, and the yield and quality of mushroom products are improved. Fuel and labor are saved by utilizing a normal-pressure energy-saving sterilization cabinet. The waste mushroom dregs produced by the common edible mushroom production are manufactured into biomass pressing block fuel, fodder, marsh gas, organic fertilizer and the like. The comprehensive factory-like circulation production method is a comprehensive circulation production and circulation utilization environment-friendly production method.

The invention relates to a microorganism composite bacterial agent applicable to the restoration of saline alkali soil polluted by petroleum, and a preparation method thereof, and belongs to the technical field of environment protection. The bacterial agent mainly comprises the following 3 components: composite microorganism bacterial liquid, nutrients, and a surfactant. The composite microorganism bacterial liquid is obtained by performing liquid culture of 3 petroleum hydrocarbon degrading bacteria of bacillus megaterium P9 strains (with a preservation number of CGMCC No. 4270), pseudomonas sp. P4 strains (with a preservation number of CGMCC No. 4269), and achromobacter xylosoxidans P2 strains (with a preservation number of CGMCC No. 4268) to obtain microorganism bacterial strains, and mixing the strains with the nutrients. The bacterial strains are preserved at China microorganism strain preservation management committee general microbiological center. The composite microorganism bacterial liquid, the nutrients and the surfactant are mixed according to a ratio of 30-200:1-2:0.2-0.5 so as to obtain the microorganism composite bacterial agent. The microorganism composite bacterial agent prepared by the invention has high removing efficiency for petroleum pollution in saline alkali soil, can both reduce the pH value of the saline alkali soil, and improve soil physical and chemical properties.

The invention discloses a tambac inducer and a preparation method thereof. The tambac inducer comprises an aquilaria sinensis tree vivo stem, a PDA culture medium, a potato sucrose culture medium and an aquilaria sinensis matrix. The method comprises the following steps of cutting off a part of an aquilaria sinensis tree vivo stem, which is forming tambac, and sterilizing, culturing, separating and identifying to obtain a tambac inducible strain; activating the strain by the PDA culture medium and fermenting by the potato sucrose liquid culture medium, and then inoculating the strain to the aquilaria sinensis matrix, and culturing for 3-5 days under the conditions of constant temperature of 30 DEG C, darkness and ventilation, thus obtain the tambac inducer. The invention has simple method, can remarkably shorten the forming time of the tambac, enhance the cultivating benefit of aquilaria sinensis trees and has favorable market application prospect.