3679 results about "Liquid culture" patented technology

A photobioreactor includes a cultivation zone configured to contain a liquid culture medium and facilitate growth of a microalgae biomass, a plurality of parallel edge-lit light transmitting devices mounted within the cultivation zone, and a collection zone oriented in relation to the cultivation zone such that at least a portion of the liquid culture medium and microalgae from the cultivation zone may be periodically harvested. Methods for illuminating algae, for dissolving materials into an algae medium, for extracting oil from algae, and for producing biodiesel from algal oil are also provided.

The present invention provides a novel method for culturing cells as well as a novel method for producing a recombinant protein by culturing cells at large scale (up to 1,500 L nominal volume and 750 L working volume), whereby an inflated bag provides a sterile, disposable cultivation chamber. The inflated bag is partially filled with liquid cultivation media and cells, and placed into a containment vessel. The containment vessel is positioned onto an orbitally shaken platform. The orbital shaking moves the containment vessel and thus the bag and induces thereby motion to the liquid contained therein (“shake mixing”). This motion (caused by orbital shaking) induces a dynamic force field that ensures cell suspension, bulk mixing, and oxygen transfer from the liquid surface to the respiring cells without damaging shear or foam generation.

The invention discloses a composite microorganism viable bacteria preparation, which contains pediococcus, yeast and bacillus with the total bacteria count of more than 1 billion per gram. The invention also discloses a method for preparing the composite microorganism viable bacteria preparation, which comprises the following steps: performing amplification liquid culture on various strains respectively according to a conventional method in the field; mixing the materials according to a required proportion; and performing solid fermentation, drying and pulverization to obtain the composite microorganism viable bacteria preparation. The invention also discloses application of the composite microorganism viable bacteria preparation in preparing an organic waste water purifying agent and an aquaculture water body purifying agent, a deodorizer, a degreasing agent or an agent for removing pipe blockages for the hygiene of a refuse tip, a public place, an agricultural product market or a kitchen, feed additives in poultry and animal husbandry or pet culture, and preparations for controlling agricultural microorganism diseases. The composite microorganism viable bacteria preparation can effectively decompose a large amount of putrescent organic matters, inhibit the growth of harmful microorganisms and eliminate peculiar smells, and has the characteristics of high ammonia nitrogen removal rate, no generation of residual sludge, effective deodorization, low cost and the like.

A method for cultivating Antrodia Camphorata is provided. First, Antrodia Camphorata is pre-incubated on a sloped surface culture medium. A portion of the pre-incubated Antrodia Camphorata is transferred to a liquid culture medium for incubation. Then, Antrodia Camphorata incubated in the liquid culture medium is moved to a solid culture medium for incubation. The cultivated Antrodia Camphorata has similar efficacies for inhibiting cancel cell growth, reducing the occurrence of atherosclerosis or recovering the damaged liver functions, as the wild Antrodia Camphorata.

A radiation sensing method and device that is used to measure physical properties of materials over a wide dynamic range. The sensor (20) comprises multiple radiation sources and multiple detectors at multiple separation distances. The detected signals from the different sources are separated and then combined mathematically in a manner such that the combination is self-compensated for both component drift and changes in radiation coupling efficiency between the source or detector and the material of interest. In a preferred embodiment, the biomass in a liquid cell culture (54) is measured with high accuracy over a wide dynamic range using optical wavelength radiation. The measurement can be made with the sensor external to the liquid culture container in a manner that is compensated for the thickness of the container window (50).

A photobioreactor includes a cultivation zone configured to contain a liquid culture medium and facilitate growth of a microalgae biomass, a plurality of parallel edge-lit light transmitting devices mounted within the cultivation zone, and a collection zone oriented in relation to the cultivation zone such that at least a portion of the liquid culture medium and microalgae from the cultivation zone may be periodically harvested. Methods for illuminating algae, for dissolving materials into an algae medium, for extracting oil from algae, and for producing biodiesel from algal oil are also provided.

The invention discloses a production method of a pig feed additive containing traditional Chinese medicine and active probiotics, which comprises the steps of: respectively conducting single-spawn strain quantity expanding culture to the parent strains of active probiotics for pigs; mixing equiponderant first-order seed liquid of all kinds of active probiotics for pigs, inoculating the liquid into seed tank culture mediums for multi-spawn mixed culture and then inoculating the liquid into traditional Chinese medicine liquid culture mediums for primary traditional Chinese medicine fermentation; and mixing multi-spawn mixed traditional Chinese medicine fermentation liquid with corn cob meal with the same volume to obtain solid fermented seeds and then inoculating the seeds into traditional Chinese medicine solid culture mediums for secondary traditional Chinese medicine fermentation. By implementing the embodiment of the invention, the strain activity of the active probiotics in the pigfeed additive and the colonization performance in intestinal canals can be greatly improved, the produced pig feed additive is rich in traditional Chinese medicine compositions, the effect of active compositions which take a pharmacological effect in the traditional Chinese medicine compositions can be fully exerted, the body strengthening and disease curing performance and the growth promoting performance of the pig feed additive are improved and therefore the use of antibiotics can be fully avoided.

The invention relates to a method for preparing a nano-cellulose antibacterial composite material through on-line culture. The method comprises the following steps of: (1) inoculating an activated bacterial cellulose producing strain into a liquid culture medium, performing amplification culture, transferring the liquid culture medium into a bioreactor, culturing, adding an antibacterial material into the liquid culture medium, and continuing to culture to obtain an unpurified antibacterial bacterial cellulose composite material; and (2) peeling a bacterial cellulose membrane from a framework or directly soaking the composite material in a NaOH solution, treating in water bath, and washing until an obtained product is neutral to obtain the nano-cellulose antibacterial composite material. The preparation efficiency is high, and the method is simple, convenient, feasible and low in cost; and the surface of the bacterial cellulose composite material has a nanoscale three-dimensional fibrous mesh-shaped structure, the tensile strength of the material is greatly improved compared with that of a pure bacterial cellulose membrane, and the material can be widely applied to products such as facial masks, wound dressings, plasters, artificial skins and the like.

The invention relates to a bacterial cellulose / polymer composite film and a preparation method thereof. The bacterial cellulose / polymer composite film is formed by compounding bacterial cellulose fibers on a polymer porous material in situ. The method comprises the following steps of: (1) activating strains, finally inoculating into a liquid culture medium to culture, infiltrating the surface of the porous polymer film in the liquid culture medium and exposing in air; (2) repeating the operation every 2-3 days until a composite film is formed; (3) post-treating the composite film to be neutral to obtain a bacterial cellulose / polymer composite gel film; and (4) drying the gel film obtained in the step (3) to constant weight to obtain the bacterial cellulose / polymer composite dry film. The invention solves the problem of low mechanical strength of a single bacterial cellulose film. By utilizing hydrogen bonding produced by hydroxyl groups on cellulose macromolecules, the strength, the durability and the water absorbability of the bacterial cellulose / polymer composite gel film and the composite dry film are improved.

The invention discloses a preparing method of new type lipopeptide biosurfactant surfactin and appliance, which comprises the following steps: utilizing bacillus subtilis strain E8 (conserved in Chinese microbe conservative council common microbe center, the keeping number at 1107) to proceed microbe liquid ferment prepare surfactant surfactin; proceeding carbon source, nitrogen source and inorganic salt optimize for the needed liquid culture medium; getting fermentation liquor; centrifuging to remove thallus; acidifying supernatant; extracting rough extract; further-extracting through dissolvent; acidifying; collecting deposition; centrifuging; vacuum-drying; getting pre-refined product; freeze-drying through drainage pillar and FPLC extraction; getting product with high purity; making the output of acidifying extraction at 24-56 h reach 5-13g / L. The dominant fermentate molecular weight of high-yield strain E8 can reach 1022Da and CMC can reach 1 mu M, which is the lowest of reported surfactin CMC value.

A solid state fermentation (SSF) method is provided which is effective for both small- and large-scale fungal cultivation. Also provided is SSF media for fungal cultivation. The media preferably contains a carbon source and nitrogen source to provide a carbon to nitrogen ratio of about 5:1 to about 25:1 by weight. The media may also contain a vitamin and an inorganic substance. A preferred SSF medium contains malt extract, yeast extract, peptone, glucose, water, solid base, and calcium carbonate or gypsum. Before propagating fungal mycelia in the SSF medium, the mycelia may be pre-cultivated in a solid culture medium and then in a liquid medium. Although the SSF method can be used in growing most fungi, preferred fungi include Cordyceps sinensis, Ganoderma lucidum, Antrodia camphorata, Trametes versicolor, and Agaricus blazei. The SSF method not only produces high yield of fungi, but also stimulates the production of fungal metabolites, particularly the kinds with pharmaceutical and medicinal activities. Cordyceps sinensis is preferably grown to produce the active compound H1A which is a derivative of ergosterol.

The invention relates to a device and method for preparing a hollow heteromorphic bacteria cellulose material. The device comprises an airtight container, a second tubular component, a first tubular component and a cylindrical hollow plug, wherein the first tubular component and the second tubular component are coaxially sheathed inside the lumen of the second tubular component; an opening of the second tubular component is sealed with the first tubular component by the cylindrical hollow plug; and the gap between the first tubular component and the second tubular component is a space for storing fermentation medium. The method comprises the following steps: after inoculating fluid nutrient medium with bacterial cellulose-producing strain for spread cultivation, transferring the bacterial cellulose-producing strain to a fermentation device with an oxygen permeability mould, and culturing to obtain the heteromorphic bacteria cellulose material. The hollow heteromorphic bacteria cellulose (BC) material prepared by the invention not only has controllable size and shape, but also has the detachable and reused mould of the fermentation device, can be widely applied to substitute goods of hollow organs, such as artificial vascular grafts, nerve fiber conduits and the like, and can be served as the material for packing the foods, such as casings for meat products, jelly coatings and the like.

An apparatus and method of stirring cells in liquid culture media is provided. Baffles are mounted along the interior wall of the container and a hump is positioned on the center of the bottom surface of the container. A stirring apparatus is positioned inside the container. The blades of the stirring apparatus are angled outward toward the bottom of the container. The bottom edge of the blades extend closer to the bottom surface of the container then the apex of the hump. The liquid in the container is at a level below the top of the blades such that the blades are moved through the surface of the liquid. A magnetic bar is attached to the shaft and is driven by an external rotating magnetic bar.

The invention provides an acid-resistant bifidobacterium breve BB8dpH and an application thereof. The nucleotide sequence of the 16SrRNA gene part of the bifidobacterium breve BB8dpH is shown in SEQ ID NO.1, and is stored with the preservation number of CGMCCNo.8370. The bifidobacterium breve BB8dpH is a strain obtained by separating from the dejecta of healthy young people and further screening under the pH 3.2 acid condition. According to the fermentation and culturing process, anaerobic culture at 37 DEG C is carried out in a BL liquid substrate (containing 0.05% cysteine hydrochloride) for 24 hours. The bifidobacterium breve BB8dpH has remarkably better acid resistance than the common strains, and the acid resistance has genetic stability; the bifidobacterium breve BB8dpH has different percent contents of cell membrane fatty acids from the common strains, the average carbon chain length of the bifidobacterium breve BB8dpH is remarkably longer than that of the common strains, and the cell membrane fluidity of the bifidobacterium breve BB8dpH is remarkably lower than that of the common strains; and the bifidobacterium breve BB8dpH is used in the production field of daily fermented food, health-care food and medicines.

Disclosed is an efficient method for culturing an edible basidiomycetous fungus such as Mushroom Agaricus Blazei Murill in a liquid culture medium to give fungus aggregates of several centimeter size. Characteristically, the liquid culture medium is formulated with sucrose as a carbon source in the form of crude cane sugar in combination with a water-insoluble growth-supporting material in the form of a fine powder to serve as the core of the fungus aggregates as selected from crushed sugarcane, sugarcane bagasse, pine trees and wheat bran. Further characteristically, the culturing procedure is carried out under an oxygen-enriched condition by blowing oxygen-enriched air of at least 30% by volume oxygen into the culture medium under pressurization at 0.12 to 0.5 MPa (absolute) in a specified blowing rate.

The invention discloses a preparation method of a hypha / nanoparticle composite sphere material. The preparation method is characterized by comprising steps as follows: preparing a culture medium; adding 4-20 ml of a nanoparticle aqueous solution containing 1-10 mg of nanoparticles per milliliter to the sterilized liquid culture medium, evenly mixing the mixture, inoculating the mixture with strains, culturing the mixture for 48-96 h under conditions that the temperature is 15-35 DEG C and rotating oscillation is performed at the speed of 80-200 r / min to form a hypha / nanoparticle composite sphere material, performing filtration to remove a liquid, soaking solids, namely, hypha / nanoparticle composite spheres, with a sodium hydroxide aqueous solution for 12 h, washing the spheres with deionized water until the spheres are neutral, and then performing freeze-drying to prepare the hypha / nanoparticle composite sphere material. The composite sphere material prepared with the method is applicable to fields of industrial catalysis, wastewater treatment, biomedicine and the like and has the characteristics of low cost, high activity, easiness in recovery and the like.

The invention discloses a preparation method for a bacterial cellulose composite membrane and application of the bacterial cellulose composite membrane as a facial mask material. The preparation method comprises the following steps: (1) selecting a strain; (2) preparing a seed containing a polyglutamic acid (PGA) and a liquid culture medium; (3) culturing seed cells; (4) producing bacterial cellulose by static liquid fermentation; and (5) purifying a bacterial cellulose membrane. The bacterial cellulose composite membrane can be used as a facial mask carrier or is directly used as a wet facial mask. The preparation method improves the yield of the bacterial cellulose by adding the polyglutamic acid (PGA) into the culture medium, improves the water content and the mechanical strength of a wet membrane, and has simple process and low cost; and the experiments prove that the method can ensure that the dry weight of the bacterial cellulose is improved to between 14 and 16g / L from the prior dry weight of between 8 and 10g / L, and the water content is increased to 99 percent from 98 percent.

The invention discloses a method for preparing a bioactive filler through immobilization of an anaerobic ammonia oxidizing bacterium, and belongs to the field of water treatment. The active filler is prepared by the following steps of: grinding active carbon, soaking in an anaerobic ammonia oxidizing bacterium liquid culture medium, and preparing an embedding solution from polyvinyl alcohol, calcium carbonate and condensed anaerobic ammonia oxidizing bacterium liquor in proportion; mixing and uniformly stirring the prepared embedding solution with soaked granular active carbon, and implementing immobilization through boric acid to finish cross-linking immobilization, thereby obtaining the active filler. The bioactive filler, after being cultured for 2 weeks, can recover anaerobic ammonia oxidizing activity. The bioactive filler disclosed by the invention is good in settlement performance and high in mechanical strength, and can achieve a micro-expansive state in an upflow biofilter.

The production of functional red rice powder with high color number and without citrinin belongs to the field of red rice product producing technology. The functional red rice powder is produced with monascus strain No. 9901 or 9906 liquid culture seed and grain material culture medium and through inoculation of liquid seed, solid fermentation at 25-32 deg.c temperature and 60-90 % humidity for 2-3 weeks, sterilization, stoving and milling. During the production, liquid fermentation process may be also adopted. The product has color number of 200-600 U / g and content of functional componentMonacolin K 4-12 g / kg with Monacolin K in open ring structure accounting for 70-90 %, and no toxic matter citrinin. The product may be used as food additive or fixing safely.

The invention is a method for using flexible packaging in connection with incubating a sample in a liquid culture medium. A flexible, gusseted bag is first presterilized by irradiation. Then, the bag is prefilled with a presterilized liquid culture medium. The bag is self-supporting and takes on the characteristics of a rigid container when filled. Prefilling and sterilizing it enables it to be shipped to a laboratory. The bag is easy to open and reseal for introduction of a culture sample at the laboratory. After introduction, the sample is incubated in the bag.

The invention discloses a repairing method of microorganism in contaminated soil of chromium slag storage yard, comprising the steps of selecting Pannonibacter phragmitetus BB single bacterium, dropping the single bacterium in a liquid culture medium containing Cr (VI) of 50-250mg / L for culturing until Cr (VI) is not detected in the culture solution, selecting strains in a bottle for transferring, gradually improving the Cr (VI) concentration for gradient domestication, adding the domesticated Pannonibacter phragmitetus BB lysate in the contaminated soil, and culturing for 4-5 days at a temperature below 30 DEG C, so as to repair the soil, wherein the amount of the added lysate is that the mass-volume ratio (g: mL) of the soil to the lysate is 1: 2. The invention treats and repairs hexavalent chromium-contaminated soil through the strains with hexavalent chromium reducing capacity, the operation is simple and feasible, the cost is low, and the method is an environment-friendly treatment method.

The invention relates to a method for culturing microalgae by an illumination way and a reactor thereof, which belong to the technical field of biology. In the invention, carbon dioxide is injected into a pipeline through a carbon dioxide feeding device during the microalgae culturing process, and microalgae liquid culture from a fermentation tank and the injected carbon dioxide are fully mixed through a circulating pump and then flow in the pipeline; as the pipeline is twisted on a cylindrical light source, the carbon dioxide can be fully utilized for photosynthesize; and the microalgae culture that flows out from the pipeline enters the fermentation tank, then is fully mixed with aerated air by stirring of a stirring vane, and then pumped into the pipeline again to form flow circulation. The bioreactor adopting the method can absorb 40-80 percent of carbon dioxide in fixed aerated air under the condition of injecting 1-40 percent of carbon dioxide, obtains the microalgae with dry cell weight concentration up to more than 3.5g / L after five-day culture and achieves the purposes of effectively absorbing carbon dioxide and culturing the microalgae.

The invention discloses a method for culturing edible fungi, which includes the following steps: culturing the mother culture in larger scale to prepare liquid culture spawn, introducing the liquid culture spawn together with culture medium which needs to be sterilized separately to cultivating material for thick solid ventilated culturing, when the cultivating material is filled with hypha, briquetting and shaping the cultivating material, and sending the briquetted cultivating material to a mushroom house to produce mushroom. The method is environment-friendly, energy-saving, short in production period, low in pollution rate and small in work intensity.

The invention relates to a comprehensive factory-like circulation production method of pleurotus eryngii, and relates to the technical field of fungus plantation. By utilizing liquid cultures, the production efficiency of the pleurotus eryngii and the little oyster mushroom, pleurotus cornucopiae or pleurotus geesteranus is greatly improved, the amount of used labor and floor space are reduced, and cost is reduced. Due to the facts that local cotton stalks are utilized for producing the pleurotus eryngii, and a large number of waste mushroom dregs produced after the pleurotus eryngii is produced serve as main cultivating materials of little oyster mushroom, pleurotus cornucopiae or pleurotus geesteranus, cost is greatly reduced, and environmental pollution is removed. A semi-underground mushroom house is adopted for full-year little oyster mushroom, pleurotus cornucopiae or pleurotus geesteranus industrialized production, the limited problem that a traditional greenhouse is affected by seasons is solved, and the yield and quality of mushroom products are improved. Fuel and labor are saved by utilizing a normal-pressure energy-saving sterilization cabinet. The waste mushroom dregs produced by the common edible mushroom production are manufactured into biomass pressing block fuel, fodder, marsh gas, organic fertilizer and the like. The comprehensive factory-like circulation production method is a comprehensive circulation production and circulation utilization environment-friendly production method.

A method of creating an extract of myceliated agricultural product for human consumption includes providing an agricultural substrate such as rice, where the agricultural substrate has been inoculated by liquid media comprising an aliquot of culture derived from liquid-state fermentation. The culture being selected from the group consisting of Basidiomycota and Ascomycota fungi. Next, the step of enabling mycelium growth on the substrate by controlling temperature, humidity and sterility of the environment. After mycelium growth on the substrate reaches a desired stage, then the step of boiling the substrate in water and separating the water-substrate mixture into aqueous component and non-aqueous components creates an extraction.

The invention relates to a microorganism composite bacterial agent applicable to the restoration of saline alkali soil polluted by petroleum, and a preparation method thereof, and belongs to the technical field of environment protection. The bacterial agent mainly comprises the following 3 components: composite microorganism bacterial liquid, nutrients, and a surfactant. The composite microorganism bacterial liquid is obtained by performing liquid culture of 3 petroleum hydrocarbon degrading bacteria of bacillus megaterium P9 strains (with a preservation number of CGMCC No. 4270), pseudomonas sp. P4 strains (with a preservation number of CGMCC No. 4269), and achromobacter xylosoxidans P2 strains (with a preservation number of CGMCC No. 4268) to obtain microorganism bacterial strains, and mixing the strains with the nutrients. The bacterial strains are preserved at China microorganism strain preservation management committee general microbiological center. The composite microorganism bacterial liquid, the nutrients and the surfactant are mixed according to a ratio of 30-200:1-2:0.2-0.5 so as to obtain the microorganism composite bacterial agent. The microorganism composite bacterial agent prepared by the invention has high removing efficiency for petroleum pollution in saline alkali soil, can both reduce the pH value of the saline alkali soil, and improve soil physical and chemical properties.

The invention relates to low-temperature-resistant petroleum degradation strains as well as a culture method, a culture medium and application thereof, wherein the low-temperature-resistant petroleum degradation strains are Paracoccus sp. D17 and Paracoccus sp. D24;a liquid culture medium for the two strains is screened, and per 1000ml of the liquid culture medium comprises 0.5g of NaCl, 0.1g of K2HPO4, 0.1g of NH4H2PO4, 0.1g of (NH4)2SO4, 0.02g of MgSO4.7H2O, 0.3g of KNO3, 700-900ml of oilfield wastewater and the balance of ultrapure water; a solid culture medium for the two strains is screened and is formed by additionally adding 15-20g of agar into each 1000ml of the liquid culture medium and further uniformly coating 20-30 mu l of sterile crude oil on the surface of the culture medium; and the two strains can be applied in degradation of petroleum hydrocarbon and bioremediation of petroleum pollution in soil or a water body. The two low-temperature strains can be applied in a low-temperature environment and have great value for controlling the petroleum pollution in the soil in the low-temperature environment.

The invention discloses a tambac inducer and a preparation method thereof. The tambac inducer comprises an aquilaria sinensis tree vivo stem, a PDA culture medium, a potato sucrose culture medium and an aquilaria sinensis matrix. The method comprises the following steps of cutting off a part of an aquilaria sinensis tree vivo stem, which is forming tambac, and sterilizing, culturing, separating and identifying to obtain a tambac inducible strain; activating the strain by the PDA culture medium and fermenting by the potato sucrose liquid culture medium, and then inoculating the strain to the aquilaria sinensis matrix, and culturing for 3-5 days under the conditions of constant temperature of 30 DEG C, darkness and ventilation, thus obtain the tambac inducer. The invention has simple method, can remarkably shorten the forming time of the tambac, enhance the cultivating benefit of aquilaria sinensis trees and has favorable market application prospect.