298 results about "Tetracycline antibiotics" patented technology

The tetracycline class of antibiotics has played a major role in the treatment of infectious diseases for the past 50 years. However, the increased use of the tetracyclines in human and veterinary medicine has led to resistance among many organisms previously susceptible to tetracycline antibiotics. The modular synthesis of tetracyclines and tetracycline analogs described provides an efficient and enantioselective route to a variety of tetracycline analogs and polycyclines previously inaccessible via earlier tetracycline syntheses and semi-synthetic methods. These analogs may be used as anti-microbial agents or anti-proliferative agents in the treatment of diseases of humans or other animals.

An aqueous based pharmaceutical composition for use as a oral hygienic treatment is described. The composition contains a pharmaceutically effective amount of a tetracycline-based antibiotic; a water-soluble calcium salt that aids in solubilizing the tetracycline-based antibiotic; a thickener; a pH adjustment agent; an antifoaming agent; an excipient; a surfactant; a preservative; and a flavoring agent. A method of making the aqueous based pharmaceutical composition includes the steps of adjusting, defoaming, dispensing, dispersing, dissolving, flavoring, heating, minimizing, pouring, solubilizing, suspending, and sweetening. A method of using the aqueous based pharmaceutical composition is also disclosed which includes the steps of expectorating, gargling, obtaining, pouring, receiving, and swishing.

Methods of treatment and dosage regimes using hydrophobic gel or foam compositions comprising a tetracycline antibiotic for accelerating the return of skin integrity and or in treating or alleviating a disorder including a wound, burn, impetigo, acne, rosacea, a skin disease caused by a bacteria or a tetracycline antibiotic responsive disease, wherein the foam composition or gel is administered topically to a target area on a subject having the disorder and wherein the target area comprises an area of skin, or mucosa or an eye.

The invention relates to a preparation method for a porous carbon material with a high specific surface area and belongs to the technical field of the preparation for an environment function material. The preparation method comprises the following steps: firstly, carbonizing a carbon source under the nitrogen protection; secondly, utilizing a chemical activating agent potassium hydroxide to activate the carbide under the high-temperature calcination condition; lastly, utilizing the hydrochloric acid to remove the impurities, washing with deionized water or hot water till the material is neutral, thereby acquiring the porous carbon material with the high specific surface area. The parameters, such as, appearance, duct distribution, and the like, of the compound material are revealed through various representing methods. The absorbing experiment is utilized to research the property of the porous carbon material for removing the tetracycline antibiotics in the water environment.

The invention belongs to the field of pollution prevention and control and discloses a method for treating a water body containing tetracycline antibiotics by using powdered activated carbon. The method comprises the steps of adding powdered activated carbon and the water body polluted by tetracycline antibiotics into a container, performing treatment by using the method of intermittent churning for a proper time, and then removing powdered activated carbon from the water body. The method has the advantages of simple operation, low cost, less time consumption, wide application area, safety and friendliness to the environment, as well as adaptability to industrialized application.

The invention discloses preparation of a mesoporous molecular imprinting fluorescent probe and detection of tetracycline antibiotics by the mesoporous molecular imprinting fluorescent probe, relates to a method for detecting tetracycline antibiotic residues, and aims to solve the problems of tedious sample preprocessing process, long analysis time and high cost of an existing method for detecting tetracycline antibiotic residues as well as the problems of poor target molecule selectivity, relatively small specific surface area and low adsorption amount of an existing molecular imprinting polymer-quantum dot composite material. The preparation method comprises the following steps: 1, preparing a molecular imprinting polymer; 2, preparing a Mn doped ZnS quantum dot; and 3, preparing the mesoporous molecular imprinting fluorescent probe. A use method comprises the following steps: adding the mesoporous molecular imprinting fluorescent probe into a sample solution containing the tetracycline antibiotics, performing ultrasonic treatment for uniform dispersion, and performing fluorescent detection. The mesoporous molecular imprinting fluorescent probe prepared with the method is high in selectivity and high in sensitivity.

The invention relates to a preparation method of a polydopamine modified ZIF-8 functionalized nanofiber membrane and application of the membrane in antibiotic adsorption. The functionalized nanofibermembrane is prepared through the electrospinning technology and a conventional solution method, polyacrylonitrile serves as a base, the surface of the polyacrylonitrile is wrapped with polydopamine toimprove the polydopamine, so that ZIF-8 crystal particles easily grow on the outermost layer of the polyacrylonitrile. The obtained polydopamine modified ZIF-8 functionalized nanofiber membrane can effectively adsorb tetracycline antibiotics from an aqueous solution, and the obtained polydopamine modified ZIF-8 functionalized nanofiber membrane has excellent recycling ability. The preparation process is simple and feasible, raw materials are easy to obtain and environmentally friendly, the cost is low, and the method has good application prospects.

The invention provides a preparing method of Tetracycline molecular imprinting column. The invention includes the following steps: dissolving template molecular Tetracycline (TC) with ultrasound in methanol, adding functional monomer and pore-forming agent (cyclohexanol and lauryl alcohol), oscillating for 6 hours, adding crosslinking agent (ethylene glycol dimethacrylate) and initiator (azobisisobutyronitrile), after ultrasonic degassing, adding nitrogen for 20 minutes, deoxidizing, pouring the solution into 10cm stainless column with bottom sealed, sealing the top end, putting the column into 60 DEG C water for water bath, after 12 hours, obtaining the molecular imprinting column by heat initiation reaction. Connecting the column to the high-pressure pump for washing away the pore-forming agent and template molecular, in the end the molecular imprinting column with good separation effect is obtained. Compared with traditional bulk polymerization method, the invention has the characteristics of simple preparation for avoiding process of lapping, good molecular recognition ability and so on. The molecular imprinting column provided by the invention is used as a liquid chromatogram filling, and can realize separation, enrichment and purification of Tetracycline antibiotics.

The invention relates to a method for detecting the residue amount of terracycline antibiotics in milk and dairy products. The method utilizes an ultra performance liquid chromatography - electrospray tandem triple quadrupole mass spectrometer to determine the residue amount of the terracycline antibiotics. The method is as follows: a sample is extracted from Na2EDTA-McIlvaine buffer solution (pH4.0); proteins are removed by trichloroacetic acids; a columella is extracted through an HLB solid phase and then purified and enriched; the sample is separated by a chromatographic column, with the column temperature of 30 DEG C; gradient elution is performed by utilization of water solution (v/v) which contains 0.1 percent of methanoic acids and acetonitrile as a moving phase; and quantitative detection is performed by adoption of the multi-reaction monitoring means. The detection limit of instruments is between 1.0 and 2.0 mu g/kg; a related coefficient r reaches over 0.999 within the linear range of between 1 and 100 mu g/kg; and the recovery rate is between 81.7 and 100.7 percent (the addition levels are 10 mu g/kg, 50 mu g/kg and 100 mu g/kg). The method has the advantages of quickness, accuracy, high sensitivity and wide application scope.

The invention discloses a process and a device for treating tetracycline antibiotic wastewater. The process comprises a pretreatment step and an anaerobic/aerobiotic treatment step; the pretreatment step comprises performing electrolytic treatment by taking ferric carbon microelectrolysis ceramsite as a filler; the anaerobic/aerobiotic treatment step comprises orderly performing anaerobic treatment and aerobiotic treatment on the wastewater treated by virtue of the pretreatment step, and fly ash ceramsite is taken as a filler for the anaerobic treatment, and sludge ceramsite is taken as a filler for the aerobiotic treatment. The invention also discloses the device for the process. According to the process and the device for treating the tetracycline antibiotic wastewater, the multi-stage treatment process is simplified, the capital investment is greatly reduced and the capital is saved; and the own characteristics of the wastewater are thoroughly utilized, and the dosage of a chemical agent is reduced, and therefore, secondary pollution is reduced while the cost is saved.

The invention discloses a trace antibiotic efficient enrichment and separation method in a water environment. The adsorption performance of magnetic graphene on tetracycline antibiotics under different adsorption conditions is studied, and the change of the antibiotic concentration in solution before and after the adsorption is detected by an ultraviolet spectrophotometer, so that the corresponding adsorption rate of the antibiotics and the magnetic graphene adsorption capacity are calculated. The influence of different factors on the adsorption performance of the antibiotics is investigated; the optimum adsorption condition is obtained; the adsorption effect mechanism is analyzed; the adsorption kinetics and adsorption thermodynamics are discussed. According to the method, tetracycline hydrochloride TC is adopted as the research object, and the adsorption characteristics of MGO on the TC under different factors of the solution pH, temperature, dissolved organic matter (taking humic acid as an example) and the like are systemically studied, in particular to the adsorption kinetics and adsorption thermodynamics of the MGO on the TC.

The invention discloses a method for performing pig manure resource treatment by utilizing hermetia illucens, belonging to the field of solid waste treatment. The method comprises the following technological steps: (1) mixing pig manure with oilseed meals so as to adjust the water content of pig manure; (2) performing primary fermentation to the mixture by adopting bacillus subtilis, and further fermenting with brewer yeast; (3) feeding black soldier fly Hermetia illucens for raising according to a certain proportion; and (4) after the raising period is finished, collecting the black soldier fly Hermetia illucens and ooze. The method is simple to operate, and fast for pig manure resource treatment process. After conducting the above technological treatment, pig manure and oil seed meals are converted into black soldier fly Hermetia illucens and excreta ooze thereof, the residual tetracycline antibiotics (oxytetracycline, chlorotetracycline and tetracycline) in the pig manure cannot be detected in the black soldier fly Hermetia illucens and ooze. The method not only can solve the problems of pig manure environment pollution, the antibiotics residues, human health damage and the like, but also can provide black soldier fly Hermetia illucens and ooze with rich nutrients for feed and fertilizer industries.

The invention discloses a gold/platinum bimetal nanocluster fluorescence probe based on polyethyleneimine protection, and an application of the fluorescence probe in aureomycin detection, and belongs to the technical field of fluorescence probes. A bimetal gold/platinum nanocluster has a great Stokes shift (about 150nm), can stably exist in a solution with extreme pH (potential of hydrogen) and high ion concentration, and releases stable fluorescence under long-time ultraviolet irradiation, so that a wide application of the nanocluster in the fields of medicines and biology is facilitated. In addition, the probe is used for detecting tetracycline antibiotics based on a mechanism of fluorescence quenching, and aureomycin can be distinguished from tetracycline by adding Al<3+> based on a mechanism of fluorescence enhancement, so that the purpose of specific aureomycin detection is achieved. The probe is used for detecting the aureomycin; a linear range is 0.5-30 micrometers; and a detection limit is 0.5 micrometers. In addition, real object detection is successfully performed on the aureomycin in milk by the fluorescence probe, and a good effect is obtained, so that the fluorescence probe can be used for detecting aureomycin residues in food.

The invention discloses a preparation method and application of a nano fiber molecularly-imprinted polymer. The preparation method is characterized by comprising the following steps: carrying out silanization treatment and denaturation on nano fiber, and synthesizing the nano cellulose molecularly-imprinted polymer by using doxycycline as template molecules, acetonitrile/methanol as a pore-forming agent, methacrylic acid as a functional monomer, trimethylolpropane trimethyl acrylate as a crosslinking agent and azodiisobutyronitrile as an initiator. The application method comprises the following steps: characterizing the synthesized polymer material, filling in a solid-phase extraction column, and determining the concentration of tetracycline antibiotics in the sample to be detected according to the standard curve in combination with high-performance liquid chromatography. The application method has the advantages of high sensitivity, high detection speed, low detection limit, high efficiency and low price.

The tetracycline class of antibiotics has played a major role in the treatment of infectious diseases for the past 50 years. However, the increased use of the tetracyclines in human and veterinary medicine has led to resistance among many organisms previously susceptible to tetracycline antibiotics. The modular synthesis of tetracyclines and tetracycline analogs described provides an efficient and enantioselective route to a variety of tetracycline analogs and polycyclines previously inaccessible via earlier tetra-cycline syntheses and semi-synthetic methods. These analogs may be used as anti-microbial agents or anti-pro liferative agents in the treatment of diseases of humans or other animals.

The invention relates to an environmental analysis detection method, and specifically, relates to a high performance liquid chromatography determination method of tetracycline antibiotic in soil. The high performance liquid chromatography determination method of tetracycline antibiotic in soil comprises the following steps of 1, a sample collecting and preparation process comprising collecting soil in a plough layer having thickness of 0 to 20 centimeters, wherein soil in a composting place and field edges is not adopted, uniformly mixing multiple single-point samples, carrying out quarteringreduction to obtain a mixed soil sample having weight of about 1kg, putting the mixed soil sample into a sealing bag, bringing the sealing bag with the mixed soil sample back with low-temperature preservation in the dark, putting the mixed soil sample into a room, drying in air, crushing, screening by a nylon sieve of 60 meshes, and weighing 2g of the mixed soil sample for next use, 2, an extractpreparation process, 3, an extraction, purification and enrichment process, and 4, an elution, volume metering and detection process. The high performance liquid chromatography determination method of tetracycline antibiotic in soil has normalized processes, reduces a vacuum drying process, produces low jamming, shortens operation time and improves operation accuracy. Through connection between astrong anion solid phase extraction column and a C18 inverted solid phase extraction column in series, purification and enrichment effects are greatly improved.

The invention discloses a modified sodium alginate adsorbing material for tetracycline antibiotics adsorption and a preparation method of the material. The method comprises the following steps that 1,sodium alginate and a solvent are mixed and stirred to be uniform according to the mass volume ratio being (1-3):(97-99), the sodium alginate is fully swelled, ultrasonic defoaming and degassing areconducted, and sodium alginate sol is obtained; 2, the sodium alginate sol is dripped into a metal salt solution with the mass concentration being 1-10 wt% at a constant speed, stirring is conducted,a cross-linking reaction is conducted for 2-24 h, filtering is conducted, drying is conducted, and the modified sodium alginate adsorbing material for tetracycline antibiotics adsorption is obtained.According to the obtained adsorbent, the raw materials are cheap and easy to obtain, the absorbent preparation process is simple, the tetracycline antibiotics removing efficiency is high, after the absorbent is used, the absorbent can be subjected to biodegradation and can be regenerated, secondary pollution cannot occur, and the modified sodium alginate adsorbing material has the good applicationprospect.

The invention relates to a fluorescence detection method for trace tetracycline antibiotics. According to the method, by utilizing the combined action of tetracycline antibiotics molecules and the specificity of an aptamer thereof, an amplification action of a rolling ring amplification signal and high selection action of a graphene molecular beacon as well as combining a fluorescence spectrophotometer, the fluorescence signal of the graphene molecular beacon is obtained, so that quantitative detection of trace tetracycline antibiotics can be realized. According to the method, a plurality of defects of the prior art such as complexity in detection are overcome, the sensitivity and selectivity are improved, and a new method is provided for detection of other small molecule substances or pollutants.

The improved clean production process of tetracycline antibiotics has the steps of concentrating and separating the first crystallized mother liquid to obtain concentrated solution; electrically crystallizing the concentrated solution and separating to obtain product while leaving the second crystallized mother liquid; concentrating and drying the second crystallized mother liquid to form solid waste; etc. The improved process recovers tetracycline antibiotics from the first crystallized mother liquid and solidifies the second crystallized mother liquid so as to raise tetracycline antibiotics yield and reduce environment protecting job.

A topical therapeutic hydrophobic breakable composition includes a carrier comprising (a) about 60% to about 99% by weight of at least one hydrophobic oil; (b) at least one viscosity-modifying agents selected from the group consisting of a fatty alcohol, a fatty acid and a wax; and (c) a tetracycline antibiotic, characterized in that at least part of the tetracycline antibiotic is suspended in the composition; the viscosity of the composition is at least about 30% higher than the viscosity of the carrier without the tetracycline antibiotic; and is higher than the viscosity of the hydrophobic oil and the tetracycline antibiotic without the viscosity modifying agents; and the amount of viscosity modifying agents can optionally be reduced by at least an amount by weight that would have increased the viscosity of the carrier without the tetracycline antibiotic by at least 30%; wherein the tetracycline is chemically stable in the composition for at least six months; wherein more than about 90% of the tetracycline has not broken down; wherein when packaged in an aerosol container to which is added a liquefied or compressed gas propellant the composition affords upon release from the container a breakable foam of at least good quality that breaks easily upon application of shear force.

The invention discloses a pearl nucleus active liquid used for saltwater pearl culture and a preparation method thereof. In the pearl culture process, by using drug-treated pearl nuclei, immunological rejection on the pearl nuclei after a pearl shell nucleus-implanting operation can be reduced, the migration and the division of small cells are irritated, the formation of pearl sacs is promoted, the wound infection after the operation is reduced, and the goal of comprehensively improving the pearl quality and yield by reducing the nucleus-spitting rate, increasing the thickness of pearl layers and improving the survival rate of cultured pearls is achieved. In the invention, the pearl nucleus active liquid is prepared by utilizing glacial acetic acid, chitosan, tetracycline antibiotics and sterilized filtered seawater. When the pearl nuclei treated by the invention are used for pearl culture, the survival rate of the cultured pearls is increased by 2.59 to 7.33 percent, the pearl-containing rate is increased by 9.8 to 20.07 percent, and the high-quality pearl rate is increased by 5.97 to 12.55 percent.

The invention discloses a method for detecting trace tetracycline antibiotics in water. The method comprises the following steps of: filtering a water sample to remove suspensions, and adjusting the pH of the water sample to 2-4; activating a hydrophile lipophile balance (HLB) solid-phase extraction column sequentially by using acetone, methyl alcohol, a methanoic acid aqueous solution containing ammonium acetate and super-pure water, taking a water sample through column in the step (1), drying the HLB solid-phase extraction column under nitrogen protection, eluting the HLB solid-phase extraction column by eluant, collecting the eluant, drying the eluant under nitrogen flow, and adding acetonitrile to dissolve residues; and quantitatively detecting the concentration of the trace tetracycline antibiotics. The method is environmentally-friendly in water sample pretreatment environment, easy to operate, high in enrichment coefficient and high in reproducibility and has the advantages of quickness, accuracy, high sensitivity, wide application range and the like.

The invention discloses a fluorescent probe, an enhanced ratio fluorescence sensor, a method and application. The fluorescent probe is used for detecting tetracycline antibiotics, and is prepared by dispersing a zeolite imidazate skeleton ZIF-8 into an aqueous solution of terpyridyl ruthenium chloride hexahydrate and reacting, wherein the fluorescent probe has an emission peak of red fluorescence at the wavelength of 604 nm, the fluorescent probe generates an emission peak of green fluorescence at the wavelength of 512 nm for the tetracycline antibiotics, and the particle size of the fluorescent probe is 73-83 nm. Herein, the enhanced ratio fluorescence sensor is used for detecting tetracycline antibiotics, has very important significance and application value for monitoring tetracycline antibiotic residues in drinking water and food, so that the fluorescent probe has the advantages of sensitivity, accuracy, rapidness, simplicity in operation and convenience in visualization.

The invention provides a high selectivity tetracycline antibiotics molecularly imprinted polymer preparation method. The high selectivity tetracycline antibiotics molecularly imprinted polymer preparation method comprises the following steps of: dissolving template molecules, methacrylic acid (MAA, 6mmol), a cross-linking agent (TRIM, 3mL) and an initiating agent in a pore-forming agent, and carrying out vacuum water bath; and collecting obtained polymer microparticles after ending reaction to obtain molecularly imprinted polymers (MIPs) for detecting antibiotic residues. The high selectivity tetracycline antibiotics molecularly imprinted polymers prepared by the method show relatively good specific adsorption capacity for tetracycline, has high adsorption capacity, is very suitable as an adsorbent for solid-phase extraction and realizes purification and enrichment of trace tetracycline antibiotics in complex samples.

The invention provides a fungus strain A. flavus G10 for degrading polyurethane plastic. The microorganism preservation number of the fungus strain A. flavus G10 is GDMCC 60537. The invention also provides a method for culturing the fungus strain A. flavus G10. The method includes the steps of isolating the fungus strain from the intestinal tract of a cricket; adopting PU as a unique carbon sourcefor culturing the fungus strain in a liquid culture medium to obtain a culture solution; diluting the culture solution and coating a solidified nutrient agar culture medium containing tetracycline antibiotics and a potato glucose agar culture medium with the culture solution to obtain cultured growing products. The cultured growing products are subcultured on fresh plates at 30 DEG C until a single fungus strain is obtained on each plate. The fungus strain A. flavus G10 can degrade the polyurethane plastic quickly.