55 results about "Cultured pearl" patented technology

A method of culturing a pearl using a mollusk having mantle tissue that includes placing a first portion of a nucleus adjacent to the mantle tissue and allowing the mollusk to deposit nacre on this first portion of the nucleus; and repositioning the nucleus so a second portion of the nucleus is adjacent to the mantle tissue and allowing the mollusk to deposit nacre on this second portion of the nucleus. Also a cultured pearl produced by a method of placing a first portion of a nucleus adjacent to a mollusk's mantle tissue and allowing the mollusk to deposit nacre on this first portion of the nucleus; and repositioning the nucleus so a second portion of the nucleus is adjacent to the mantle tissue and allowing the mollusk to deposit nacre on this second portion of the nucleus. Further, a method of cultivating a pearl using one or more mollusks each having mantle tissue that includes placing a nucleus adjacent to the mantle tissue of one of the mollusks and sequentially reorienting the nucleus with respect to the mantle tissue of either this mollusk or one or more other mollusks.

The invention discloses a method for artificially culturing seawater anucleate pearl. The method comprises the following steps: taking out the mantle epithelium of a healthy nacre, removing the skirt band part, digesting the mantle epithelium in protease solution, then adding the culture solution with shell blood serum to stop the digestion, culturing the culture solution containing shell tissue fluid and a property amount of penicillin and streptomycin in a sterile environment for 5 to 30 hours, then injecting the cell suspension into a pearl breeding shell body, and putting the pearl breeding shell body in the seawater for suspended culture or into a culture pond box for feeding after a recreation period. The method has little damage to the pearl breeding shell, a high survival rate and a high pearl formation rate. The cultured pearl is suitable for medicinal use. The method has convenient operation and low cost.

A topical application comprises cultured pearl powder of particle size ranging between 0.4 μm and 1 μm ground by an ultrasonic gas jet mill, and purified water suspending the cultured pearl powder.

The invention discloses an in-vitro culture method for the pearl shell mantle tissue and a special culture solution thereof. The culture medium comprises hydrolyzed pearl liquor, shell liquor, antibiotics and seawater. The method conventionally comprises the following steps: taking the material from a nacre to obtain the mantle epithelium, removing the skirt band part, cutting the tissue into tissue blocks with the size of about 1mm to 2mm after purification treatment, putting the tissue blocks in the protease solution for digestion, and adding the culture solution to stop the digestion. The preliminarily digested tissue blocks are respectively put into a culture dish or cling to the pearl core which is covered by adherence substance. The cultured pearl shell mantle tissue grows in good conditions; after 4 hours, the cells are emigrated from the tissue blocks; and after 2 days in-vitro pearl sacs are formed effectively. The method can be used to inject the anucleate pearl sacs at different positions in the pearl shell with the inserted pearl nucleus so as to culture the anucleate pearl. The method has low cost, a high survival rate, a high pearl formation rate, and little damage to the nacre.

The subject invention provides methods for producing cultured pearls in gastropods, such as the queen conch, Strombus gigas, and other gastropod species (e.g., families Strombidae, Haliotididae and Volutidae), with high success rates. The subject invention also provides cultured pearls produced by the methods as described herein.

The invention discloses a pearl nucleus active liquid used for saltwater pearl culture and a preparation method thereof. In the pearl culture process, by using drug-treated pearl nuclei, immunological rejection on the pearl nuclei after a pearl shell nucleus-implanting operation can be reduced, the migration and the division of small cells are irritated, the formation of pearl sacs is promoted, the wound infection after the operation is reduced, and the goal of comprehensively improving the pearl quality and yield by reducing the nucleus-spitting rate, increasing the thickness of pearl layers and improving the survival rate of cultured pearls is achieved. In the invention, the pearl nucleus active liquid is prepared by utilizing glacial acetic acid, chitosan, tetracycline antibiotics and sterilized filtered seawater. When the pearl nuclei treated by the invention are used for pearl culture, the survival rate of the cultured pearls is increased by 2.59 to 7.33 percent, the pearl-containing rate is increased by 9.8 to 20.07 percent, and the high-quality pearl rate is increased by 5.97 to 12.55 percent.

The invention discloses a method for culturing large pearls by using clam visceral sac implanted nucleuses. The method is characterized by comprising the following steps of: determining three nucleus implanting positions, namely a ridge nucleus position, an abdominal foot nucleus position and an annular intestine nucleus position capable of culturing the pearls in clam visceral sacs according to the size and physique conditions of clams, then implanting 1 to 3 pearl nucleuses and small cell fragments into the selected position in the three nucleus positions, putting the clams implanted with nucleuses back to a culture water area to culture the pearls, and harvesting free large pearls from the visceral sacs of the clams after the cultured pearls are mature. The method for implanting the nucleuses and culturing the pearls by selecting the three key nucleus implanting positions of the visceral sacs of the clams, namely the ridge nucleus position, the abdominal foot nucleus position and the annular intestine nucleus position effectively improves the pearl forming rate by fully using the characteristics of large gonad positions of the three nucleus positions adaptive to culturing the pearls, few intestines and visceral organs affecting nucleus implantation and the like; the nucleus implanted mother clams have extremely low death rate and high implanted pearl nucleus retention rate and high-quality pearl rate; and the method has remarkable economic benefit and social benefit, and can be applied to scale production.

The invention discloses a factory-like pearl culture system. With worsening of seaculture environments, seashore benches and neritic regions are no longer the only place for pearl culture, and factory-like pearl culture is a good choice. The factory-like pearl culture system is composed of a water pump house, an algal culture pond, a seawater filtering tank, a fresh water filtering tank and a pearl culture pond. Pearl shell seedlings are put in the pearl culture pond, filtered seawater according with seawater salinity, power of hydrogen and temperatures is injected into the pearl culture pond through a pipeline, seawater with algae is also injected into the pearl culture pond through a pipeline, and the algae are used for pearl shell culture. The factory-like pearl culture system integrates scientific research, ecology, culture and demonstration, factory-like pearl culture fundamentally solves the problem of environmental pollution in sea farming in the prior art and is free from influences of natural environment, tests show that the survival rate of pearls cultured through the factory-like pearl culture system reaches more than 95%, the rate of finished products also reaches more than 95%, and the factory-like pearl culture system represents one development direction of pearl culture.

The invention discloses a pearl cultivating method for nuclear pearl, a tool adopting the method and cultivated pearl. The pearl cultivating method for nuclear pearl comprises the following steps: nuclear-free pearl is used as a pearl nucleus or a shell is grinded to form the pearl nucleus; the pearl nucleus is arranged on a cell sheet and then the pearl nucleus with the cell sheet is implanted on viscera or pelecypodium of a mussel or a pearl shell with a tool; the mussel and the pearl shell is restored in water for culture; the mussel or the pearl shell are opened and a pearl formed around the pearl nucleus is harvested. As the pelecypodium of the mussel or the pearl shell is above a visceral sac and has large space compared with the visceral sac or a mantle, and the pelecypodium secretes more nacre, the pearl nucleus cannot be spit out easily if the pearl nucleus is implanted on the pelecypodium and the diameter of the pearl nucleus is expanded, moreover, the mussel or the pearl shell secretes more nacre after the pearl nucleus is coated. The pearl cultivating method ensures that the pearl nucleus can be inserted into the pelecypodium accurately, the cell sheet can wrap the pearl nucleus better and the pearl nucleus has no need of punching a hole. The pearl has large particle, good translucent luster, short growth cycle and high economic value.

The invention provides a nucleus inserting tool for freshwater mussel breeding pearls. The tool comprises a handle, a conducting component, a pearl nucleus pike-off driving component, a guide rod inserting component, a telescopic driving component and a return trigger component, wherein a first mounting groove is formed in the handle, the pearl nucleus poke-off driving component is movably mounted in the first mounting groove through a first spring, and a material poke U-shaped tune is arranged at one end of the pearl nucleus poke-off driving component; a positioning groove is formed in the side wall of the telescopic driving component, the return trigger component is matched with the positioning groove, and an auxiliary driving push block component is arranged on the guide rod inserting component; a needle is arranged at the end of the guide rod inserting component, and the material U-shaped tube is matched with the needle. The conducting component is used for guiding and positioning, the guide rod inserting component conducts positioning on a pearl nucleus to which cell small pieces adhere, and the pearl nucleus poke-off driving component conveys the pearl nucleus to the nucleus inserting position. The nucleus inserting tool is easy to operate, combines conducting, positioning and nucleus inserting, does not need to be replaced by operating tools and can improve the nucleus inserting efficiency.

The invention relates to a nondestructive testing device for identifying authenticity of a pearl. The nondestructive testing device is characterized by comprising a halogen tungsten lamp source, an optical fiber spectroscope, mechanical components for fixing the optical fiber spectroscope, a fixing component, a manual mobile platform in vertical direction, mechanical components for fixing the manual mobile platform, an optical platform in vertical direction, an optical fiber connection plate, a lens, a reference lens, a PZT motor, an NI card, a directional manual mobile platform, turbinate cup components used for loading the pearl to be tested, a directional automatic mobile scanning platform and a controller thereof, a near-infrared spectrometer and a computer, wherein the fixing component is provided with the optical fiber connection plate and the lens; the NI card is used for controlling the PZT motor. The nondestructive testing device can two-dimensionally scan the pearl and can imagine the internal structure of the pearl. The nondestructive testing device not only can identify real and fake pearls, but also can identify natural seawater pearls or freshwater cultured pearls, and has high test precision.

The invention discloses a pearl nucleus having a mantellum cell hydrogel layer at the surface and a preparation method therefor, and belongs to the field of production of artificial cultured pearls. The preparation method comprises separating mantellum cells of a pearl mussel into individual free cells through enzymolysis, premixing the individual free cells in a high-molecular aqueous solution, and making the high-molecular aqueous solution gel on the surface of a pearl nucleus to form hydrogel containing mantellum cells and being almost uniform in thickness. By adopting the preparation method, the mantellum cells can be almost uniformly distributed at the periphery of the pearl nucleus, the problem that mantellum cuticle cells are not uniform in distribution on the periphery of a pearl nucleus by adopting a traditional method of implantation of small particle of mantellum cells can be solved.

The invention discloses a pearl coloration liquid for mariculture comprising colorant, surface active agent and dissolvent, wherein the concentration of the colorant and the surface active agent is 0.4-0.6g / L. Its preparation comprises weighing the colorant, choosing the corresponding dissolvent for dissolving, charging corresponding surface active agent, stirring to dissolve the latter, keeping constant volume with the corresponding dissolvent, and adjusting the pH to 8.00-8.60 with alkali.

The invention discloses a method for pearl culture. The method comprises the following steps that: firstly, culturing pearl mussels with bead nuclei in a culture pond in a density of 1,000 to 1,200 mussels per mu, wherein the water depth is controlled to be 1.4 to 1.6 meters first; when the water temperature is more than or equal to 10 DEG C, putting live foods in the culture pond according to the standard of 30 to 40 kilograms per mu; pumping water to the culture pond by a water inlet so as to increase the water depth to 1.7 to 1.9 meters; and discharging water in the culture pond by a water outlet so as to restore the water depth to be 1.4 to 1.6 meters, controlling the time interval between the water charge starting time and the water discharge ending time to be 8 to 12 days, preferably 10 days, and before the cultured pearl is mature, repeating the water charge-discharge step. The method has the advantages that: the investment risk is effectively reduced, the environmental pollution is reduced, the pearls cultured by the method quickly grow, the efficiency is high, and the pearls cultured by the method are high in brightness and have beautiful appearances.

The invention discloses a pearl bleaching liquid for mariculture characterized by using sea water as solvent, using surface active agent polyethylene glycol dimethyl ether and bleaching agent hydrogen peroxide solution as solute, wherein the volume percentage concentration of the two solutes are 0.1-0.3% and 4-6%, the pH of the bleaching bath is 8.00-8.60. The preparation comprises measuring bleaching agent hydrogen peroxide and charging into surface active agent polyethylene glycol dimethyl ether, stirring to dissolve the latter, charging in seawater, and finally adjusting the pH of the solution.

The invention provides methods and compositions useful in pearl oyster cultivation. Polynucleotide and polypeptides relating to the nacre gene of Pinctada margaritifera are provided. Antibodies related to these polypeptides, and compositions comprising polynucleotides, polypeptides and / or antibodies of the invention are also provided. The invention provides methods of using these polynucleotides, polypeptides and antibodies, including methods related to pearl oyster cultivation. Arrays comprising polynucleotides, polypeptides and / or antibodies of the invention are also provided.

The invention relates to a wind-and-wave-resistant pearl oyster farming device and a lifting type ecological farming method using the same. The wind-and-wave-resistant pearl oyster farming device comprises a farming raft and a foundation framework which is used for carrying the farming raft and can regulate the underwater penetration of the farming raft, wherein the farming raft comprises a plurality of floating ropes and oyster containing net cages which are respectively connected below the corresponding floating ropes and form an integrated extension rope clamping cage with all the floating ropes, the farming raft is connected on the foundation framework through the floating ropes, and the underwater penetration of the oyster arranging net cage can be further regulated by regulating the vertical lifting positions of the floating ropes on the foundation framework. As the farming raft comprises the plurality of the floating ropes, the integrated oyster arranging net cage formed with the floating ropes and the like and the farming rapt is connected on the foundation framework in a manner of being capable of regulating the underwater penetration, when the farming raft is used, a farming water layer can be flexibly regulated, then the wind-and-wave-resistant pearl oyster farming device can facilitate production operation and be conductive to creating an artificial ecological environment for farming the oysters and growing cultured pearls, and production and economic losses caused by typhoons can be further avoided.

The invention discloses a pearl bleaching solution and a preparation method of the pearl bleaching solution. The pearl bleaching solution is prepared by hydrogen peroxide, a neutralizer, a solvent, a stabilizer, a composite emulsifier and distilled water. The pearl bleaching solution comprises the components in parts by weight as follows: 70-200 parts of hydrogen peroxide, 2-3 parts of neutralizer, 360-480 parts of solvent, 0.1-0.5 parts of stabilizer, 3-10 parts of composite emulsifier and 400-550 parts of distilled water. The pearl bleaching solution prepared by the invention is mainly used to bleach cultured pearls and the bleached pearls have low pit rate, uniform color and luster and good brightness.

Invention provides a method for preparing big black shell mantle flakes, which comprises the following steps: putting big black shells for preparing the flake into low-temperature sea water to perform freezing treatment before slicing so that the big black shells are in a shock or dead natural ringent state and mantles lose reaction and shrinkage capabilities as well as mucilaginous capability to an operation stimulus; and after opening the shell, slicing the mantle flakes one by one on the shell directly. The method greatly reduces the difficulty for preparing the big black shell mantle flakes and simplifies the operation process, and the prepared mantle flakes have good effect of growing cultured pearls.

The invention discloses a method for cultivating pearls in a test tube. The method includes the first step of getting down the exocuticle of a margin membrane of the pallium of a baby clam in a membrane stripping mode, the second step of digesting the exocuticle to form epithelium cell sap, the third step of preparing a culture medium, the fourth step of carrying out cell culture to enable cells to be attached to the nucleus to form a complete pearl cultivation sac, and the fifth step of implanting the pearl cultivation sac into the body of a pearl cultivation clam for pearl cultivation. According to the method, pallium cells are cultivated in the test tube, the cells are evenly attached to the pearl nucleus to form the complete pearl cultivation sac, and then the pearl cultivation sac is implanted into the body of the pearl cultivation clam for pearl cultivation; the probability that in the prior art, the small pallium can not grow evenly to form the complete pearl cultivation sac due to operation errors or other reasons, so that nacre secreted by the pearl cultivation sac is not uniform, and bad-quality pearls such as dirty pearls, tailed pearls and pearls with burnt heads are produced is reduced, and the quality of the cultured pearls is effectively improved.

The invention relates to the technical field of pearl culture, in particular to a nuclear implanting surgery method capable of sending a nucleus and a piece simultaneously. It is guaranteed that the nucleus implanted into a pearl shell body and the small piece of a mantle cell can be attached closely, the phenomenon that the small piece of the mantle cell is not closely attached to the nucleus, and plain pearls and tail pearls are produced is avoided, and the quality of cultured pearls is improved.

The subject invention provides methods for producing cultured pearls in gastropods, such as the queen conch, Strombus gigas, and other gastropod species (e.g., families Strombidae, Haliotididae and Volutidae), with high success rates. The subject invention also provides cultured pearls produced by the methods as described herein.

A magnetic cultured pearl comprises a magnetic core member around which pearl nacre material has been permitted to develop as a result of implantation of the magnetic core member within a host organism. The magnetic cultured pearls can be used to manufacture pearl necklaces or pearl bracelets without the need to string the cultured pearls upon conventional strings. A method of manufacturing the magnetic cultured pearls is also disclosed.

The invention provides a pearl pre-processing and classifying system for pearl culture. The pearl pre-processing and classifying system for pearl culture comprises a processing box, sliding grooves are formed in the two sides of the inner wall of the processing box, and a connecting hole is formed in the back face of the processing box. The pearl pre-processing and classifying system for pearl culture has the advantages that the size range of screened pearls can be conveniently adjusted to adapt to centralized discharging of pearls in different size ranges, the number of movable partition plates can be adjusted and fixed according to use requirements, the classifying range and number can be conveniently adjusted, the collecting range is conveniently adjusted, the system is simple in adjusting structure and convenient to use, the size ranges of pearls can be screened and sorted according to the specific use requirements of a user, classifying labor and cost are saved, classifying efficiency and quality are improved, two sets of pushing rods are matched with two sets of adjusting sliding rods, so that two groups of pearls are conveniently and synchronously pushed and classified, andthe screening efficiency is improved.

The invention belongs to the technical field of fresh water pearl mussel aquaculture and discloses an environmentally-restorable fresh water pearl mussel aquaculture based water conservation fishery method which involves an aquaculture pond for pearl mussel aquaculture. A balance biogroup is released in the aquaculture pond which is partitioned into a balance area and a static aquaculture area bya partition net in the aquaculture pond. Directional fertilization and sampling integrated rods are released into the aquaculture pond. By release of the balance biogroup into the aquaculture pond tobalance a fresh water pearl mussel aquaculture water environment of the aquaculture pond, growth of fresh water pearl mussels and pearls is promoted, fertilizing quantity reduction, aquaculture cost reduction and effective improvement of water quality are realized under identical conditions. In addition, by arrangement of the partition net for separation the balance biogroup and the pearl mussels,the directional fertilization and sampling integrated rods are used for feeding the pearl mussels, the balance biogroup and the pearl mussels are prevented from scrambling for feed, pearl mussel feedconsumption is reduced, and aquaculture cost is saved.

The invention discloses a method for culturing pearls. The method is characterized in that within a certain culturing time, the pearl mussel culturing density is reduced correspondingly with the extension of the culturing time, that is, 2000 pearl mussels are cultured per mu in the first year, 1500 pearl mussels are cultured per mu in the second year, 1000 pearl mussels are cultured per mu in the third year, and 500 pearl mussels are cultured per mu in the fourth year and later. Hyriopsis cumingii is cultured with a mesh bag hanging method, five pieces of the hyriopsis cumingii are cultured per bag in the first year, four pieces of the hyriopsis cumingii are cultured per bag in the second year, three pieces of the hyriopsis cumingii are cultured per bag in the third year, and two pieces of the hyriopsis cumingii are cultured per bag in the fourth year and later. According to the culturing method, on one hand, the culturing water surface can be used reasonably according to sizes of the pearl mussels, on the other hand, the pearl mussels are cultured in a relatively suitable water body all the time, and a good water body environment is created for the pearl mussels, so that growth of the pearl mussels and protection of the ecological environment are facilitated, and the pearl culturing benefit is increased.

The invention discloses a method for artificially culturing seawater anucleate pearl. The method comprises the following steps: taking out the mantle epithelium of a healthy nacre, removing the skirt band part, digesting the mantle epithelium in protease solution, then adding the culture solution with shell blood serum to stop the digestion, culturing the culture solution containing shell tissue fluid and a property amount of penicillin and streptomycin in a sterile environment for 5 to 30 hours, then injecting the cell suspension into a pearl breeding shell body, and putting the pearl breeding shell body in the seawater for suspended culture or into a culture pond box for feeding after a recreation period. The method has little damage to the pearl breeding shell, a high survival rate anda high pearl formation rate. The cultured pearl is suitable for medicinal use. The method has convenient operation and low cost.

The invention relates to a culture method of luminous pearl. The culture method comprises the following steps: selecting immature and healthy seawater pinctada martensi or freshwater hyriopsis cumingii of one year's old as a mother shell; cutting down cell slices from the pinctada martensi or hyriopsis cumingii; taking fluorite granules ground into round as pearl nucleus; implanting the fluorite granules and the cell slices as pearl nucleuses in the stomach of the mother shell; culturing the mother shell with seawater or freshwater for 7-9 months to form nacre on the pearl nucleus, and takingout the mother shell to obtain the luminous pearl. Fluorite is used as the pearl nucleus, the cultured pearl can shine in a dark place, the cultured pearl has high hardness and is not easy to wear, the product gloss is high, bleaching and brightening tretament is not required, and the luminous pearl is good in environmental protection performance, harmless to the human body and suitable for wearing.

The invention provides a cultivating method for increasing content of trace element of pearls. In the water body for cultivating pearls, the content of trace element needed in target pearl cultivationis improved so as to obtain pearls containing one or a plurality of trace elements. The invention overcomes the defects that the trace element is limit in the natural water body environment and the content of trace element of cultivated pearls is low and is even lack. The invention can cultivate pearls which are rich in trace elements or a plurality of trace elements, and the content of the target trace element can be improved by above 20% so as to improve the drug and edible quality of pearls, self-quality and selling price, improve the economic benefit of pearl cultivators and enhance the market competitiveness of the freshwater pearls. The invention is easy and simple to learn, has low cultivating cost and strong applicability, is favorable for popularizing and applying, in particularto various pearl farmers, and increases the financial income of pearl farmers.