9 results about "Pinctada margaritifera" patented technology

The utility model relates to the technical field of aquaculture, and discloses a pinctada fucata recirculating aquaculture device. The recirculating aquaculture device comprises a pool body; the base plate is located at the top of the pond body and used for driving the breeding cage to automatically move to the outside of the pond body; the two sides of the outer wall of the pond body are connected with a supporting frame through fasteners, the interior of the supporting frame is connected with the two ends of a threaded column through a rotating shaft, the threaded column is connected with one side of an air cylinder through threads, and the top of the air cylinder is connected with a base plate through fasteners, so that the bottom of a breeding cage is cushioned at the top of a collecting tank; the rope knots at the bottoms of the breeding cages are opened, the pinctada fucata in the breeding cages are shaken off into the collecting grooves below, water drips into the grooves through the through holes, and the pinctada fucata slides into the hoppers through the collecting grooves, so that feeding of the breeding cages and the pinctada fucata can be completed, and workers do not need to manually carry the support for feeding; time and labor are saved, and the working efficiency is effectively improved.

The present invention discloses a Pinctada martensii C-type lectin PmPLP, as well as its application in the preparation of drugs for inhibiting microorganisms and in promoting the release of pro-inflammatory factors induced by nucleation in Pinctada martensii. The present invention conducts preliminary research on the identification and immune function of Pinctada martensii C-type lectin PmPLP to determine the immune response of PmPLP molecules in the immune response of Pinctada martensii against the invasion of external pathogens, and to understand the antibacterial activity of PmPLP after in vitro expression, providing data support and research basis for the study of the immune defense mechanism of Pinctada martensii and other marine mollusks C-type lectins. The present invention conducts in-depth research on the immune response mechanism of Pinctada martensii, which helps to reveal its molecular regulatory mechanism in response to pathogen invasion, and is of great significance for improving aquaculture efficiency and disease prevention and control.

The present invention provides a winter shed culture method for autumn nucleus-planted pearl oysters, belonging to the technical field of aquaculture. The method of the present invention comprises the following steps: setting up a winter shed for aquaculture ponds, respectively setting up a unicellular algae culture pond and a nucleus-planted pearl oyster culture pond, and simultaneously carrying out unicellular algae culture and nucleus-planted pearl oyster culture in the winter shed; adding manganese sulfate to the algae culture solution, and soaking the cell pieces and pearl nuclei with arachidonic acid solution; after the nucleus-planting operation is completed, the nucleus-planted pearl oyster is moved to the nucleus-planted pearl oyster culture pond for recuperation for 15 to 18 days; after the recuperation is completed, the pearl oyster continues to be cultured in the nucleus-planted pearl oyster rest pond until the water temperature in the sea area rises to ≥24°C for 5 consecutive days, and then is moved to the sea area for cultivation. The method of the present invention can improve the culture survival rate and the thickness of the pearl layer of autumn nucleus-planted pearl oysters of pearl oysters, can improve the economic benefits of pearl production of pearl oysters of pearl oysters, and is easy to promote and apply in production enterprises, with significant economic and social benefits.

The application provides a SNP molecular marker related to low-temperature resistance of Pinctada martensii and application thereof, and belongs to the technical field of aquatic genetic breeding. The molecular marker comprises one or more of SNP351, SNP352, SNP362, SNP365, SNP367, SNP378, SNP396, SNP456, SNP718, SNP823 and SNP831. The molecular marker can be used for genotype identification of parent shellfish before seed breeding, and the low-temperature resistance of offspring population can be improved by screening parent shellfish with multiple advantageous genotypes.

This invention discloses a molecular marker array, microarray, and its applications for Pinctada martensii. The molecular marker array comprises 31,053 molecular markers, the physical locations of which were determined by sequence alignment based on the Pinctada martensii reference genome CNP0002248. The SNP sites in the Pinctada martensii molecular marker array of this invention are highly representative, polymorphic, and versatile. Furthermore, it covers sites and functional sites significantly associated with traits such as shell length, shell width, shell height, shell weight, shell color, pearl color, and nacre thickness. It can be used for gene identification and functional analysis of important economic traits in Pinctada martensii, research on population genetic diversity, germplasm resource conservation, genetic improvement, and marker-assisted breeding, to cultivate new varieties with excellent economic traits and promote the healthy and sustainable development of the marine pearl industry.

The invention discloses a pinctada fucata martensii body surface mucus antioxidant active peptide as well as preparation and application thereof. The amino acid sequence of the pinctada fucata martensii body surface mucus antioxidant active peptide is Arg-Trp-Ala-Ser-Trp. The preparation method of the active peptide comprises the following steps: collecting pearl shells, collecting mucus, collecting supernate, identifying the supernate and screening and determining the active peptide. The antioxidant active peptide obtained from the pinctada fucata martensii body surface mucus has high activity, free radicals can be well removed, and damage of H2O2 to skin fibroblasts is relieved; the skin wound healing can be promoted. The antioxidant active peptide has a good prospect when being applied to drugs or instruments for relieving cell oxidative stress.

The invention discloses a method for culturing a low-temperature-resistant variety of Pinctada martensii by using LncRNA as a molecular marker and application of the method, and relates to the technical field of aquatic culture, and the key points of the technical scheme are as follows: the method comprises the following steps: extracting genome DNA from the low-temperature-resistant Pinctada martensii strain; amplifying a DNA (deoxyribonucleic acid) fragment containing the lnclont allele by using a PCR (polymerase chain reaction) technology; then detecting the amplified DNA fragment by using a specific molecular marker technology, and determining whether an lnrun allele exists or not; carrying out statistical analysis on data obtained by molecular marker analysis, and judging the low-temperature tolerance of the pinctada martensii individuals; and finally, screening out low-temperature-resistant pinctada martensii individuals according to a data processing result, and performing biological verification to ensure the low-temperature resistance of the pinctada martensii individuals. When the method is used for screening the low-temperature-resistant pinctada martensii, the screening period can be shortened, the screening efficiency can be improved, the screening cost can be reduced, and the screening accuracy can be improved.

This invention discloses an antioxidant active peptide from the mucus of the Hepu pearl oyster, its preparation, and its application. The amino acid sequence of the antioxidant active peptide from the Hepu pearl oyster mucus is Arg-Trp-Ala-Ser-Trp. The preparation method of the active peptide includes collecting pearl oysters, collecting mucus, collecting supernatant, identifying the supernatant, and screening and identifying the active peptide. The antioxidant active peptide obtained from the mucus of the Hepu pearl oyster exhibits high activity, effectively scavenging free radicals and reducing H2O2 damage to skin fibroblasts; it can also promote skin wound healing. The application of this antioxidant active peptide in drugs or devices for reducing cellular oxidative stress shows good promise.

The invention discloses a Pinctada martensii C-type lectin PmPLP, an application of the Pinctada martensii C-type lectin PmPLP in preparation of drugs for inhibiting microorganisms and an application of the Pinctada martensii C-type lectin PmPLP in promotion of release of pro-inflammatory factors induced by nucleus implantation of Pinctada martensii. According to the invention, preliminary research is carried out on identification and immune functions of Pinctada martensii C-type lectin PmPLP, so that immune response of PmPLP molecules in immune response of Pinctada martensii for resisting invasion of external pathogenic bacteria is determined, and antibacterial activity of PmPLP after in-vitro expression is known; and data support and a research basis are provided for researching C-type lectin immune defense mechanisms of pinctada martensii and other marine mollusks. The immune response mechanism of the pinctada martensii is deeply studied, the molecular regulation mechanism of the pinctada martensii for coping with pathogen invasion is disclosed, and the method has important significance for improving the breeding efficiency and preventing and controlling diseases.