The preparation method comprises the following steps: extracting an intershell fluid active substance, extracting
extracellular small vesicles as an active substance target, using
hyriopsis cumingii pallium for conventional pearl
mussel breeding, and preparing the bioactive preparation for
skin injury repair from the
raw material, so as to obtain the bioactive preparation for
skin injury repair. The method comprises the following steps: collecting liquid in a gap between a pearl culture
clam shell and an outer mantle, separating and extracting vesicles from the liquid through ultracentrifugation, taking freshwater
pearl shell culture by-products as a sustainable source of bioactive substances,
grinding the bioactive substances contained in the freshwater
pearl shell culture by-products, optimizing components through
molecular composition, and preparing the freshwater
pearl shell culture by-products. A preparation with a stable
bioactive substance structure for repairing
skin injury radiated by
ultraviolet rays is obtained. The
extracellular small vesicles are extracted from pearl shells, the main components of the preparation are biological sources, the safety is high, the absorbability of a nanoscale molecular structure is good, an innovative solution is provided for
skin repair, and high value-added conversion of pearl shells is achieved.