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A cultivation method and pearl technology, applied in the field of unshaped pearl cultivation, can solve the problems of lack of pearl cultivation methods, inability to realize large-scale and industrialized production, etc., achieve high economic value and practical value, easy recovery of wounds and wound surface small effect
Inactive Publication Date: 2012-05-23
HUNAN UNIV OF ARTS & SCI +1
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Summary
Abstract
Description
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Problems solved by technology
[0003] However, the existing technology lacks the cultivation means of this kind of pearls, and cannot realize large-scale and industrialized production.
Method used
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Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0016] Embodiment 1: (1), cut off the marginal membrane of river mussel or pearl oyster, cut off the color line, take the outer skin of the mantle, and put it into a petri dish filled with Hanks solution;
[0017] (2) Take out the outer skin of the mantle, put it into a tissue masher, and mash it for 3 minutes to form a slurry;
[0018] (3) Absorb the above slurry into a centrifuge tube, let it settle naturally for 3 minutes to obtain a suspension, and set aside;
[0019] (4) Transfer the above suspension to another centrifuge tube, discard the larger tissue pieces, and set aside;
[0020] (5) Use a syringe to extract the above-mentioned suspension and inject it into 30 places of the mantle or 1 place of the viscera mass of the triangular clam, and the injection volume of each place is 0.05ml.
Embodiment 2
[0021] Embodiment 2: (1), cut off the marginal membrane of river mussel or pearl oyster, cut off the color line, take the outer skin of the mantle, and put it into a petri dish filled with Hanks solution;
[0022] (2) Take out the outer skin of the mantle, put it into a tissue masher, and mash it for 5 minutes to form a slurry;
[0023] (3) Absorb the above slurry into a centrifuge tube, let it settle naturally for 5 minutes, and obtain a suspension for later use;
[0024] (4) Transfer the above suspension to another centrifuge tube, discard the larger tissue pieces, and set aside;
[0025] (5) Use a syringe to extract the above-mentioned suspension and inject it into 50 places of the mantle or 5 places of the viscera of the triangular clam, and the injection volume of each place is 1.00ml.
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Abstract
The invention provides a culture method of amorphous pearls. The method comprises the following steps of: (1) cutting off edge membranes of freshwater mussels or pearl oysters, cutting off color lines, taking pallium exocuticles and putting the pallium exocuticles in a culture dish containing a Hanks liquid; (2) taking the pallium exocuticles out of the culture dish and mashing the pallium exocuticles for 3-5 minutes to obtain pulp; (3) sucking the pulp into a centrifuge tube, and naturally settling for 3-5 minutes to obtain a suspending liquid; (4) transferring the suspending liquid into another centrifuge tube and removing larger tissue blocks to obtain the final suspending liquid for layer use; and (5) pumping the suspending liquid by using an injector and injecting the suspending liquid into palliums or visceral masses of the freshwater mussels or pearl oysters by using the injector. In the production process of non-nucleated pearls, the culture method has the advantages that the wound is small; the wound can be healed easily and the survival rate of the freshwater mussels or pearl oysters is high; in the production process of nucleated pearls, the problems of uneven flakelet wrapping, uneven nacre secretion or flakelet falling off can be overcome; and the secretion capability of nacre of the freshwater mussels or pearl oysters can be improved greatly and the production cycle can be reduced by more than 20%, therefore, the culture method has the advantage of high beneficial and practical value.
Description
technical field [0001] The invention relates to the field of aquaculture, in particular to a method for cultivating amorphous pearls. Background technique [0002] At present, in the existing pearl cultivation, the outer skin of the mantle is cut to make small pieces of cells, the size of which is between 2mm and 6mm, and then transplanted into inoculated mussels for cultivating seedless or nucleated pearls. The pearls produced by this method are occasionally irregular and irregular, and these pearls have high use and economic value in specific occasions. [0003] But lack the cultivation means of this pearl in the existing technology, can't realize the production of large-scale and industrialization. Contents of the invention [0004] For the problems referred to above, the object of the present invention is to provide a method for cultivating amorphous and multi-shaped pearls. [0005] A kind of cultivation method of unshaped pearl of the present invention, its concret...
Claims
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Application Information
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