Management method for promoting cutting rooting of acanthopanax

Abstract

The invention provides a management method for promoting cutting rooting of acanthopanax senticosus, and relates to the technical field of artificial breeding of acanthopanax senticosus. The management method mainly comprises the steps that a soaking solution prepared from indoleacetic acid, pyroligneous liquor and walnut green seedcase extract and a disinfectant prepared from quick lime and cooked rice milk are arranged to treat cutting branches, and river sand, a walnut green seedcase treatment material, perlite and organic fertilizer are arranged as cutting media to conduct cutting treatment on the acanthopanax senticosus. According to the method, the defects in the prior art are overcome, the survival rate of acanthopanax branch cuttage is effectively increased, cuttage rooting of acanthopanax is further promoted, the growth efficiency of cuttage seedling raising is improved, and later transplanting and planting are facilitated.

Description

Technical Field

[0001] The invention relates to the technical field of artificial propagation of Acanthopanax senticosus, and in particular to a management method for promoting rooting of Acanthopanax senticosus cuttings. Background Art

[0002] Acanthopanax senticosus is a deciduous shrub of the Araliaceae family and the genus Acanthopanax. Its roots, stems and leaves can be used as medicine and food. It has the effects of invigorating qi and strengthening the spleen, nourishing the kidney and calming the mind. It can be used to treat hypertension, diabetes, rheumatism and other diseases. In recent years, Acanthopanax senticosus has gradually been developed and utilized as a raw material for medicine and food, beverage, tea and wine processing. With the increase in the development and utilization of Acanthopanax senticosus, wild Acanthopanax senticosus resources have been damaged to a certain extent. The "Chinese Plant Red Book" published in 1992 has classified Acanthopanax senticosus as an endangered species. Therefore, artificial breeding of Acanthopanax senticosus has been an important research direction in recent years.

[0003] Artificial propagation of Acanthopanax mainly includes seed germination and cutting seedlings. Since Acanthopanax seeds are of incomplete embryo development and have the characteristics of congenital dormancy, the overall germination rate of artificial sowing seedlings is low, and the early seed storage is also cumbersome, so the generally widely used seedling method is cutting seedlings; however, there are certain rooting inhibitors in the buds and barks of Acanthopanax to varying degrees, making it difficult for Acanthopanax cuttings to take root, and the natural rooting rate is only 14%-20%, and the overall cutting rate is The survival rate is not high. The commonly used method is to treat the Acanthopanax branches with plant growth hormones or rooting powder before cuttings. This can greatly improve the survival rate of cuttings and promote the rooting of Acanthopanax cuttings to a certain extent. However, the overall rooting effect is still not ideal, which has a certain impact on the survival effect of subsequent Acanthopanax transplanting. Therefore, how to further improve the survival rate and rooting effect of Acanthopanax cuttings is an important research direction for Acanthopanax cutting seedling cultivation at this stage. Summary of the invention

[0004] In view of the shortcomings of the prior art, the present invention provides a management method for promoting the rooting of Acanthopanax senticosus cuttings, which effectively improves the survival rate of Acanthopanax senticosus branch cuttings, further promotes the rooting of Acanthopanax senticosus cuttings, improves the growth efficiency of cutting seedlings, and facilitates later transplanting and planting.

[0005] To achieve the above objectives, the present invention is implemented through the following technical solutions: A management method for promoting rooting of Acanthopanax senticosus cuttings, the management method comprising the following steps: S1. Preparation of cutting branches: Select semi-lignified or lignified branches of the current year, retain 2-3 buds, and trim to retain 10-15 cm as cutting branches for standby use; S2. Soaking the lower end of the cuttings: vertically insert the lower end of the cuttings into the soaking solution for 20-30 minutes, then take it out and place it naturally for 2-3 hours. The ingredients of the soaking solution are: indoleacetic acid 100-200 mg / L, wood vinegar 10-20 mL / L, walnut green peel extract 15-20 mg / L; S3, pretreatment of cuttings: immerse the cuttings after the natural placement in a mixture of quicklime and cooked rice slurry for 5-10 min, then take them out and rinse them in clean water for 1-3 times, and pretreat the cuttings for standby use; S4. Prepare the cutting medium according to the following mass parts: 6-10 parts of river sand, 10-12 parts of walnut green peel treated material, 4-6 parts of perlite, and 8-10 parts of organic fertilizer; S5. Cutting treatment: Set up a greenhouse, insert the lower end of the pre-treated cuttings into the cutting medium, then water thoroughly, spray with carbendazim solution once every 3-5 days, and spray with clean water in time to keep the relative humidity in the greenhouse at 80%-90%.

[0006] Preferably, when pruning the branches in step S1, the upper end is cut flatly and the lower end is cut obliquely at an angle of 30-60°.

[0007] Preferably, the preparation method of the walnut green peel extract comprises the following steps: S2-1, clean the walnut green peel and cut it into small pieces of 1-2 cm, then add 4-5 times the volume of clean water to increase the pressure to 0.4-0.6Mpa, keep it at 50-60℃ for 60-80min, and then filter to obtain the filtrate and filter residue for later use; S2-2. Add 5 times the volume of 95% ethanol solution to the filter residue, heat it in a water bath at 60-70°C for 30-50 min, filter it, combine the two filtrates, concentrate them in a vacuum, and then freeze-dry them to obtain the walnut peel extract.

[0008] Preferably, in the quicklime and cooked rice slurry mixture in step S3, the concentration of quicklime is 10%-15%, and the concentration of cooked rice slurry is 20%-40%.

[0009] Preferably, the cooked rice slurry in step S3 is the filtrate obtained by grinding rice, adding 3-5 times the volume of clean water, boiling at 100-110° C. for 40-50 min, and then filtering.

[0010] Preferably, the method for preparing the walnut green peel processed material in step S4 comprises the following steps: S4-1. Wash the walnut peel and cut it into small pieces of 1-2 cm, then add 4-5 times the volume of clean water to increase the pressure to 0.4-0.6Mpa, keep it at 50-60℃ for 60-80min, and then filter it to obtain the filter residue for later use; S4-2, add 5 times the volume of 95% ethanol solution to the filter residue, heat it in a water bath at 60-70°C for 30-50 minutes, filter it, remove the filtrate, and dry the remaining solid residue in an oven to obtain dry walnut green peel material for later use; S4-3, placing the above-mentioned dried walnut green peel material in a microwave oven at 600W for 2-3 minutes, and then crushing it through a 4-5 mesh sieve to obtain walnut green peel processed material.

[0011] Preferably, the drying temperature in the oven in step S4-2 is 50-60°C, and the moisture content of the dried walnut green peel material is ≤8%.

[0012] Preferably, the mass concentration of the carbendazim solution sprayed in step S5 is 0.1%.

[0013] Preferably, in step S5, the shading rate of the greenhouse is set to 60%-70%, and the temperature in the greenhouse is controlled between 15-28°C.

[0014] The present invention provides a management method for promoting rooting of Acanthopanax senticosus cuttings, which has the advantages of: The invention adopts indoleacetic acid, wood vinegar and walnut green peel extract to soak the cutting branches in the early stage, and then adopts quicklime and cooked rice slurry for soaking treatment, which can effectively improve the survival rate of the Acanthopanax senticosus cuttings in the early stage, and combines with the subsequent addition of walnut green peel treatment material in the cutting medium, so as to further promote the early rooting of the Acanthopanax senticosus branches and promote the growth of the overall cutting seedlings thereof, which is beneficial to the survival of subsequent transplantation. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a schematic diagram of the soaking liquid treatment of Acanthopanax senticosus cuttings in an embodiment of the present invention; Figure 2 Schematic diagram of the disinfectant treatment of Acanthopanax senticosus cuttings in an embodiment of the present invention; Figure 3 This is a schematic diagram of Acanthopanax senticosus cuttings in an embodiment of the present invention; Figure 4 This is a schematic diagram of the growth of Acanthopanax senticosus cuttings 20 days after experimental group 1 in the embodiment of the present invention; Figure 5 This is a schematic diagram of the growth of Acanthopanax senticosus cuttings 20 days after experimental group 6 in the embodiment of the present invention; Figure 6 This is a schematic diagram of rooting of Acanthopanax senticosus cuttings 30 days after the experimental group 1 in the embodiment of the present invention; Figure 7 This is a schematic diagram of rooting of Acanthopanax senticosus cuttings 30 days after cuttings in Experimental Group 6 (left) and Experimental Group 5 (right) in the examples of the present invention. DETAILED DESCRIPTION

[0016] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention is clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. Embodiment 1:

[0017] 1. Walnut green skin processing: 1.1. Preparation of Extract A and Processed Material A: (1) Wash the walnut peel and cut it into small pieces of 1-2 cm, then add 5 times the volume of clean water to increase the pressure to 0.5 MPa, keep it at 55°C for 70 minutes, and then filter it to obtain the filter residue for later use; (2) Add 5 times the volume of 95% ethanol solution to the filter residue, heat it in a water bath at 65°C for 40 min, filter it, combine the two filtrates, concentrate them in a vacuum, and then freeze-dry them to obtain extract A. Then, place the remaining solid residue in an oven at 55°C and dry it to a moisture content of ≤8% to obtain dry walnut green peel material for later use; (3) The dried walnut green peel material was placed in a microwave oven at 600 W for 3 min, and then crushed through a 4-mesh sieve to obtain processed material A.

[0018] 1.2. Preparation of treated material B: Steps (1)-(2) are the same as in 1.1 above, and step (3) is omitted. The dried walnut green peel material is directly ground and crushed and passed through a 4-mesh sieve to obtain processed material B.

[0019] 2. Disinfectant configuration: 2.1 Disinfectant A: (1) Grind the rice, add 3 times the volume of water, cook at 110° C. for 30 min, and filter the filtrate to obtain cooked rice slurry for later use; (2) Prepare a solution with a concentration of 20% cooked rice slurry and 10% quicklime as disinfectant A.

[0020] 2.2 Disinfectant B: (1) Grind the rice, add 5 times the volume of water, cook at 110° C. for 50 min, and filter the filtrate to obtain cooked rice slurry for later use; (2) Prepare a solution with a concentration of 40% cooked rice slurry and 15% quicklime as disinfectant B.

[0021] 2.3 Disinfectant C: A quicklime solution with a quicklime mass concentration of 10% is disinfectant C.

[0022] 3. Preparation of soaking solution: Refer to Table 1 below to configure different soaking solutions: Table 1

[0023] 4. Preparation of cutting medium: Refer to Table 2 below to configure different cutting substrates (organic fertilizer is cow dung fertilizer): Table 2 Embodiment 2:

[0024] Acanthopanax cuttings: (1) Select semi-lignified or lignified branches of the current year, retain 2-3 buds, prune to retain a length of 10-15 cm, cut flat at the top and cut obliquely at the bottom at an angle of 30-60° to obtain cutting branches for use; (2) Insert the lower end of the cutting branch vertically into the soaking liquid for 20-30 minutes, then take it out and place it naturally for 2-3 hours. Then, soak the cutting branch in the disinfectant for 5-10 minutes before cutting, take it out and rinse it with clean water twice to obtain the pre-treated cutting branch; (3) Place the cutting medium in a seedling box made of non-woven fabric, and insert the pretreated cutting branches into the seedling box in a greenhouse with a shading rate of 60%-70%. Then water it thoroughly and spray it with a 0.1% carbendazim solution after 3 days. Spray water in time, control the relative humidity in the greenhouse to 80%-90%, and control the temperature in the greenhouse to 15-28℃. Detection:

[0025] Referring to the cutting method of Example 2 above, greenhouse cuttings were carried out on May 10, 2024, and experimental groups 1-6 were set up, with 1000 Acanthopanax senticosus cuttings in each group, and different soaking solutions, disinfectants and cutting substrates were used in each group, as shown in Table 3 below: Table 3

[0026] 1. The survival of each group of cuttings was observed 15d, 30d, and 60d after cutting. The specific results are shown in Table 4 below: Table 4

[0027] It can be seen from the above table that the Acanthopanax senticosus cuttings in experimental group 1 and experimental group 2 have a higher survival rate.

[0028] 2. The rooting rates of the Acanthopanax senticosus of each group of cuttings were tested at 15d, 30d and 60d after cuttings (100 surviving Acanthopanax senticosus were selected for root observation and statistics each time the rooting rate was tested). The specific results are shown in Table 5 below: Table 5

[0029] From the above table, we can see that experimental group 1 and experimental group 2 have the best rooting effect within 60 days.

[0030] 3. The average number of roots and average root length of each group of Acanthopanax senticosus rooted 60 days after the above-mentioned cuttings were detected (10 roots of Acanthopanax senticosus rooted in each group were selected to take the average value). The specific results are shown in Table 6 below: Table 6

[0031] From the above table, we can see that the number and length of roots of Acanthopanax senticosus cuttings in experimental groups 1 and 2 are the best.

[0032] In the actual cuttings, the growth of Acanthopanax senticosus cuttings in each group was observed. The growth of Acanthopanax senticosus cuttings in experimental groups 1 and 2 was significantly better than that in experimental group 6.

[0033] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A management method for promoting rooting of Acanthopanax senticosus cuttings, characterized in that: The management method comprises the following steps: S1. Preparation of cutting branches: Select semi-lignified or lignified branches of the current year, retain 2-3 buds, and trim to retain 10-15 cm as cutting branches for standby use; S2. Soaking the lower end of the cuttings: vertically insert the lower end of the cuttings into the soaking solution for 20-30 minutes, then take it out and place it naturally for 2-3 hours. The ingredients of the soaking solution are: indoleacetic acid 100-200 mg / L, wood vinegar 10-20 mL / L, walnut green peel extract 15-20 mg / L; S3, pretreatment of cuttings: immerse the cuttings after the natural placement in a mixture of quicklime and cooked rice slurry for 5-10 min, then take them out and rinse them in clean water for 1-3 times, and pretreat the cuttings for standby use; S4. Prepare the cutting medium according to the following mass parts: 6-10 parts of river sand, 10-12 parts of walnut green peel treated material, 4-6 parts of perlite, and 8-10 parts of organic fertilizer; S5. Cutting treatment: Set up a greenhouse, insert the lower end of the pre-treated cuttings into the cutting medium, then water thoroughly, spray with carbendazim solution once every 3-5 days, and spray with clean water in time to keep the relative humidity in the greenhouse at 80%-90%.

2. A management method for promoting rooting of Acanthopanax senticosus cuttings according to claim 1, characterized in that: In the step S1, when pruning the branches, the upper end is cut flatly, and the lower end is cut obliquely at an angle of 30-60°.

3. A management method for promoting rooting of Acanthopanax senticosus cuttings according to claim 1, characterized in that: The preparation method of the walnut green peel extract comprises the following steps: S2-1, clean the walnut green peel and cut it into small pieces of 1-2 cm, then add 4-5 times the volume of clean water to increase the pressure to 0.4-0.6Mpa, keep it at 50-60℃ for 60-80min, and then filter to obtain the filtrate and filter residue for later use; S2-2. Add 5 times the volume of 95% ethanol solution to the filter residue, heat it in a water bath at 60-70°C for 30-50 min, filter it, combine the two filtrates, concentrate them in a vacuum, and then freeze-dry them to obtain the walnut peel extract.

4. A management method for promoting rooting of Acanthopanax senticosus cuttings according to claim 1, characterized in that: In the step S3, the concentration of quicklime in the quicklime and cooked rice slurry mixture is 10%-15%, and the concentration of cooked rice slurry is 20%-40%.

5. A management method for promoting rooting of Acanthopanax senticosus cuttings according to claim 1, characterized in that: The cooked rice slurry in step S3 is obtained by grinding rice, adding 3-5 times the volume of clean water, boiling at 100-110° C. for 40-50 minutes, and then filtering the filtrate.

6. A management method for promoting rooting of Acanthopanax senticosus cuttings according to claim 1, characterized in that: The preparation method of the walnut green peel processed material in step S4 comprises the following steps: S4-1. Wash the walnut peel and cut it into small pieces of 1-2 cm, then add 4-5 times the volume of clean water to increase the pressure to 0.4-0.6Mpa, keep it at 50-60℃ for 60-80min, and then filter it to obtain the filter residue for later use; S4-2, add 5 times the volume of 95% ethanol solution to the filter residue, heat it in a water bath at 60-70°C for 30-50 minutes, filter it, remove the filtrate, and dry the remaining solid residue in an oven to obtain dry walnut green peel material for later use; S4-3, placing the above-mentioned dried walnut green peel material in a microwave oven at 600W for 2-3 minutes, and then crushing it through a 4-5 mesh sieve to obtain walnut green peel processed material.

7. A management method for promoting rooting of Acanthopanax senticosus cuttings according to claim 6, characterized in that: The drying temperature in the oven in step S4-2 is 50-60° C., and the moisture content of the dried walnut green peel material is ≤8%.

8. A management method for promoting rooting of Acanthopanax senticosus cuttings according to claim 1, characterized in that: The mass concentration of the carbendazim solution sprayed in step S5 is 0.1%.

9. A management method for promoting rooting of Acanthopanax senticosus cuttings according to claim 1, characterized in that: In step S5, the shading rate of the greenhouse is set to 60%-70%, and the temperature in the greenhouse is controlled between 15-28°C.

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