519 results about "Indole acetic acid" patented technology

The invention belongs to the technical field of agriculture and environmental pollution improvement and relates to a heavy metal resistant plant endogenous nodule bacterium and application thereof. The heavy metal resistant nodule bacterium is preserved at the China center for type culture collection (CCTCC) with the preservation date to be 2012/9/18 and culture preservation number to be CCTCC NO:M2012357. The bacterial strain liquid preparation contains more than a billion of effective living bacteria per milliliter, and the bacterial strain solid preparation contains 0.2 billion of effective living bacteria per gram. The heavy metal resistant nodule bacterium strain can secrete plant hormones indole acetic acid (IAA) and siderophores, and resists heavy metal copper and cadmium. When plants are planted in moist soil or tailings containing heavy metal and bioremediation preparation is inoculated, the heavy metal resistant nodule bacterium can promote plant growth and absorbs heavy metal copper, can improve plant restoration efficiency, can fix sandy soil and reduces water and soil loss.

The invention discloses bacillus megaterium and an application thereof, and belongs to the field of microorganisms. A plant growth promoting bacterium (bacillus megaterium JX15) is collected in the China General Microbiological Culture Collection Center on Dec.20, 2011, the class name is bacillus megaterium and the collection number is CGMCC No.5622. The bacillus megaterium has the beneficial effects that a strain can produce indoleacetic acid with high yield, can be used for nitrogen fixation and can grow by using sparingly-soluble phosphates as phosphorous sources, so as to improve the utilization rates of fertilizers, promote plant root development and absorption of the fertilizers, and increases the contents of soil mineral nitrogen and available phosphorus; the strain has a good effect on promoting growth of peanuts; the high-yield indoleacetic acid promotes the growth and the development of the peanuts; and the nitrogen and phosphate fertilizer utilization rates of the peanuts are relatively high due to the increasing of the contents of the soil mineral nitrogen and the available phosphorus.

The invention discloses an efficient tissue culture and rapid propagation technology for seedlings of bletilla striata. According to the technology, bletilla striata seeds are sown on a seed germination culture medium for sterile germination, the obtained seedlings are inoculated to a multiplication and subculture medium one by one for multiplication and subculture, clustering seedlings are obtained and inoculated to a rooting culture medium for rooting culture, the obtained tissue culture seedlings are planted on a prepared seedling hardening substrate, and the seedlings are transplanted to a large filed for plantation after seedling hardening, wherein the seed germination culture medium comprises MS (magnesium sulfate), 30g/L of sugar, 8g/L of agar and 10g/L-12g/L of potatoes; the multiplication and subculture medium comprises MS, 1.0 mg/L of KT (kinetin), 0.2 mg/L of NAA (naphthalene acetic acid), 30g/L of sugar and 9g/L of agar; the rooting culture medium comprises MS, 0.2 mg/L of NAA, 1.0mg/L of IAA (indole acetic acid), 30g/L of sugar, 9g/L of agar and 25-30 g/L of potatoes; the seedling hardening substrate comprises medium moor peat soil, perlite and wood dust in the volume ratio being 5:2:3. The technology is mature, a system is perfect, the problems of low natural germination rate of bletilla striata and difficulty in obtaining of seedlings can be effectively solved, and the technology has better popularization and application value and broad market prospect.

The invention belongs to the technical field of planting, and discloses a high-yield planting method for improving the disease resistance of Citrus. The high-yield planting method comprises the following steps: 1) selecting and processing cultivating land, selecting the sandy land for tilling, and applying sufficient decomposedmanure, digging holes according to the row spacing of (2-3) * (3-4)m, applying2-5 kg of superphosphate and 2-3 kg of plant ash for per hole; 2) transplanting and planting; transplanting and plating between late August and early September, or between February and March, and applying nutrient solution at the same time; 3) applying fertilizer; applying compound fertilizer in the germination period by 0.5-1.2kg/tree; applying calcium orthophosphate in the florescence period by 0.3-0.6kg/tree, and applying boric acid with a mass concentration of 0.3-0.5% and disease-resistance nutritional agents with the mass concentration of 0.3-0.5% by means of foliar spraying; applying foliar fertilizer with a concentration of 0.3-0.5% during the fruit expansion stage; applying fruit expanding fertilization during full bearing period by 0.5-1.5kg/tree; 4) insect killing; preparing the insect-resist agents from probiotics, indole acetic acid, Chinese medicine extract and carbendazim. The high-yield planting method for improving the disease resistance of citrus has the advantages that the yield and quality of citrus is significantly improved; the disease-resistance of citrus is improved and the fragrance and the sweetness of the fruits are greatly improved.

The invention discloses scion root liquid of hackberry. Each liter of root liquid contains the following components in parts by weight: 1-3 parts of 6-benzyl amino adenine, 2-3 parts of ABT2 rooting powder, 15-20 parts of heteroauxin, 20-30 parts of naphthylacetic acid, 0.2-0.5 part of potassium iodide, 2-4 parts of zinc sulfate, 0.05-0.1 part of sodium molybdate, 10-20 parts of ammonium nitrate, 1-3 parts of magnesium sulfate, and 1-1.5 parts of vitamin D. A method for grafting and seeding the hackberry by the root liquid comprises the steps of: selecting scions and preparing the scions; selecting a stock and cutting the selected stock; connecting; and finally, carrying out seeding management. The root liquid disclosed by the invention is reasonable in formula, and the survival ratio of the grafted hackberry can be improved to over 90%. The grafting method disclosed by the invention is simple and convenient to operate, and easy to popularize.

The invention provides a compound auxin for inducing plants to form aquatic roots and a method for forming aquatic roots, which consists of the following components in proportions by weight: 0.5-2 parts of indole butyric acid, 0.5-2 parts of indole acetic acid, 0.25-1 part of naphthaleneacetic acid and 1-4 parts of potassium dihydrogen phosphate. In the present invention, by selecting indole butyric acid, indole acetic acid and naphthalene acetic acid in an appropriate ratio, and adding the nutrient potassium dihydrogen phosphate, the training time of water rooting can be shortened, and the probability of inducing plants to form water rooting can be increased. And the formed aquatic root system has high vitality and developed root system.

The invention discloses a disease-prevention and growth-promoting Trichoderma harzianum and an application thereof. The strain number of the Trichoderma harzianum is TM2-4, which is registered in theGeneral Microbiology Center of China Microbial Culture Collection Management Committee as CGMCC No. 15881. The Trichoderma harzianum M2-4 can inhibit Botrytis cinerea, Prunus persica, Colletotrichum gloeosporioides and Fusarium oxysporum f. Sp. Cucumerinum on fruits and vegetables, and has good control effect on tomato gray mold in vitro and can promote the germination of tomato seeds and the growth of tomato plants. The Trichoderma harzianum TM2-4 has indoleacetic acid (IAA)-producing activity, ferrophilic activity, glucanase and chitinase-producing activity. The Trichoderma harzianum TM2-4 has high breeding speed and can be cultured artificially, and the culture condition is simple, and the spore production is up to 3.2 * 109 cfu/g on the solid fermentation medium of wheat bran and corncob nutrient solution.

The invention relates to a method for cultivating corn in saline-alkali soil. The method includes: broad irrigating the saline-alkali soil in the middle of March with the irrigation amount of 60-100m<3> each mu, airing for 1-1.5 months to allow earth surface to be dried enough for entering, applying 1000-1500kg of miscellaneous manure or 50-55kg of composite fertilizer to each mu, performing rotary tillage once to twice to mix the soil and the fertilizer, raking to preserve soil moisture, and levelling soil surface; processing with seed coating before sowing corn, adding pesticide first during germicide and pesticide seed dressing, piling and stuffing for 3-6 hours, then adding germicide, and sowing immediately after seed dressing, wherein the seed coating comprises actinomycin, bacillus subtilis, azospirillum, fulvic acid, carry powder, indole acetic acid, trace elements, alpha-pimacol growth regulator, EDTA-2Na chelating agent, humic acid and turf.

The invention provides Bacillus velezensis K01 and application thereof. The Bacillus velezensis K01 has the growth promotion functions of dissolving phosphorus and generating indoleacetic acid, cellulase, protease and the like, also can generate anti-bacteria active substances and volatile anti-bacteria substances, can inhibit growth of various pathogenic bacteria, has a good biocontrol function, also can favorably realize colonization on the rhizosphere of a plant and in the plant, can be applied to plant growth promotion and biological control of plant diseases, and has a good application prospect.

There is provided a series of substituted acyl guanidines of Formula (I)

or a stereoisomer; or a pharmaceutically acceptable salt thereof, wherein R₁, R₂, R₃, R₄, R₅, R₆, R₇, and R₈ as defined herein, their pharmaceutical compositions and methods of use. These compounds inhibit the processing of amyloid precursor protein (APP) by β-secretase and, more specifically, inhibit the production of Aβ-peptide. The present disclosure is directed to compounds useful in the treatment of neurological disorders related to β-amyloid production, such as Alzheimer's disease and other conditions affected by anti-amyloid activity.

The invention provides bacillus aquimaris L-60 capable of effectively prompting the growth of crops and application thereof. The strain is separated from potato rhizosphere, and is classified into bacillus aquimaris based on physiological-biochemical characteristics and 16S rDNA (ribosome Deoxyribose Nucleic Acid) analysis. As proved by a high performance liquid chromatography analysis result, the strain L-60 has a high indole Acetic acid (IAA) producing capability. As proved by a pot experiment result, a growth-promoting bio-fertilizer researched on the basis of the strain can remarkably promote the growth of corn, cucumber and tomato plants.

The invention discloses a medicago pseudomonas strain capable of producing ACC (1-aminocyclopropane-1-carboxylate) deaminase and application of the medicago pseudomonas strain, and relates to a medicago pseudomonas strain and application thereof. The pseudomonas (pseudomonas sp.) MJM-9 is preserved in the general microorganism center of the China microorganism culture preservation management committee in 26th July, 2012, and the preservation number of the pseudomonas MJM-9 is CGMCC NO.6294. The medicago pseudomonas strain containing the ACC deaminase is used as bacterial manure for growing crops. The medicago pseudomonas strain can generate the ACC deaminase, secrete auximone IAA (indole acetic acid), synthesize siderophores, reduce the concentration of ethylene inside a plant under the condition of environmental stress, promote the plant to grow fast, particularly promote growth and development of a root system of the plant, and increase utilization rate of fertilizers. Besides, when the strain is applied to agricultural production, growth of plants can be promoted, yield of crops can be increased, and stress resistance of the crops is improved.

The invention discloses the structure, the preparation process and the usage of a novel 3-substituted indole compound .The invention is characterized in that carbon 3-position of the indole is introduced with the selenium and is then connected with other substituents, which improves the stronger plant growth regulating function relative to the indole acetic acid. The compound of the invention can also be taken as an enriched selenium agent of the plant.

The invention discloses an indole-3-acetic acid molecularly imprinted magnetic cellulose microsphere and a preparation method and applications thereof, which relate to a nano magnetic cellulose molecularly imprinted microsphere and a preparation method and applications thereof. The invention is designed for solving the problem that an existing IAA (indole-3-acetic acid) detection method is complicated in step, time-consuming, quantitative in jamming and high in cost. The indole-3-acetic acid molecularly imprinted magnetic cellulose microsphere disclosed by the invention is prepared from an IAA, 4-VP, silanized beta-CD (beta-cyclodextrin), DMSO (dimethylsulfoxide), nano magnetic cellulose microsphere particles, a crosslinking agent, styrene, an initiator and water. The method comprises the following steps: 1, activating cellulose; 2, dissolving the cellulose; 3, preparing nano Fe3O4 magnetic liquid; 4, preparing nano magnetic cellulose microsphere particles; 5, silanizing beta-cyclodextrin; and 6, preparing the IAA molecularly imprinted magnetic cellulose microsphere. The indole-3-acetic acid molecularly imprinted magnetic cellulose microsphere disclosed by the invention, as an adsorbent, is used for quantitatively detecting the content of an indole-3-acetic acid in plant tissues.

A cosmetic and dermatological composition comprises 2, 3-dihydro-4-hydroxy-2-oxo-1-H-indole-3-acetic acid (4-hydroxy-1-oxindole-3-acetic acid) as an active ingredient for use as a melanin inhibitor and as an anti-tyrosinase enzyme inhibitor. This composition is excellent as a skin whitening agent. Since it also has potent antioxidant and anti-irritant activities it is useful in a cosmetic composition for improving hyperpigmentation disorders of the skin.

The invention relates to a pseudomonas mediterranea strain and application thereof. The pseudomonas mediterranea MJM-3 is preserved in the general microorganism center of the China microorganism culture preservation management committee, the preservation address is No.3 of No.1 courtyard of Beichen West road of Chaoyang district of Beijing City, the preservation date is 26th July, 2012, and the preservation number is CGMCC NO.6292. The pseudomonas mediterranea strain is used as bacterial manure for growing crops. The pseudomonas mediterranea strain has ACC (1-aminocyclopropane-1-carboxylate) deaminase activity, and can secrete auximone IAA (indole acetic acid) and siderophores. Accordingly, the concentration of ethylene in a plant under the condition of environmental stress can be reduced, growth and development of a root system of the plant are promoted, iron absorption of the plant is also promoted, and growth condition of the plant is improved. Besides, when applied to agriculturalproduction, the pseudomonas mediterranea strain can promote growth of plants, yield of crops is increased, and stress resistance of the crops is strengthened.

The invention discloses a method for rapidly breeding potted carnation through tissue culture. The method comprises the following steps of: cutting an explant and sterilizing; carrying out primary culture, wherein a primary culture medium comprises MS (Murashige and Skoog), 1.5mg/L of 6-BA (6-Benzylaminopurine), 0.2mg/L of NAA (Naphthalene Acetic Acid), 30g/L of sucrose and 8g/L of agar; carrying out subculture, wherein a subculture medium comprises MS, 0.8mg/L of 6-BA, 0.2mg/L of NAA, 30g/L of sucrose and 8g/L of agar; carrying out rooting culture, wherein a rooting culture medium comprises MS, 0.2mg/L of NAA, 0.1mg/L of IAA(Indole Acetic Acid), 30g/L of sucrose and 8g/L of agar; and washing the culture mediums away and then holding the temperature and humidity for seedling hardening and transplanting. The method is simple and easy to operate, simple in procedure and high in working efficiency, and is capable of effectively holding excellent properties of parents. According to the method, the differentiation rate of a tissue culture seedling is increased. The method has the characteristics of high multiplication speed, high rooting rate, high survival rate and low cost.

The invention discloses a cotton seedling culturing pesticide fertilizer composition. The cotton seedling culturing pesticide fertilizer composition is prepared by mixing mepiquat chloride, indoleacetic acid, azoxystrobin, metalaxyl, 15 to 25 mass percent of grass carbon, borax, zinc sulfate, monopotassium phosphate, magnesium sulfate, monoammonium phosphate and ammonium sulfate; the cotton seedling culturing pesticide fertilizer with the brand new composition integrates multiple functions of fertilizer efficiency, sterilization and disinfection, chemical regulation, rooting promotion and the like, saves labor and time, is low in cost, innocent, high in fertilizer efficiency, and high in utilization rate, and inhibits vain growth at a seedling stage, controls soil-borne disease at the seedling stage, promotes root development, and resists drought and low temperature. The seedling does not wilt, is high in disease resistance and the production of the cotton is increased obviously.

The invention discloses a high-activity nutrition nursery stock flower rooting agent. The high-activity nutrition nursery stock flower rooting agent comprises the following raw materials: 3-4 parts of 4-iodiphenoxyaceticacid, 0.5-1.5 parts of zinc sulfate, 1-3 parts of naphthylacetic acid, 3-7 parts of indole acetic acid, 20-30 parts of powdered steatile, 1-3 parts of vitamin B12, 4-8 parts of an additive, 2-4 parts of boron oxide, 4-8 parts of ammonium nitrate, 2-3 parts of vitamin D3, 5-7 parts of humic acid, 0.6-1.4 parts of compound sodium nitrophenolate, 3-6 parts of an antiseptic, 0.5-1.2 parts of hydration metal salt, and 10-14 parts of a nutrition additive. The rooting agent can accelerate breeding of plant cells, improves plant physiological function, accelerates main root growth and lateral root formation, greatly shortens the rooting time, and obviously increases rooting rate, in addition, the rooting agent also contains trace elements, accelerates nursery stock flower nutrition growth, promotes deep roots and luxuriant leaves, and healthy growth, and increases the survival rate of the cutting seedlings. The rooting agent has the advantages of low production cost, concise process, and has high practical value and good application prospect.

The present invention relates to a brevibacillus laterosporus-containing antibacterial yield increase composition, which is an antibacterial yield increase composition comprising effective active components such as an active component A and an active component B, wherein the active component A is selected from brevibacillus laterosporus, and the active component B is any one compound selected from benzylaminopurine, brassinolide, indole acetic acid, triacontanol and gibberellic acid. The composition provides high activity for a plurality of plant diseases on a plurality of crops, and has characteristics of significant synergy effect, bacterial killing spectrum expanding, increase of effective fertilizer absorption of crops, helping of good growth of crop inferior parts, and regulation of water balance in crops so as to activate metabolic systems of crops, and promote crop cell growth and root system activity strengthening.

The invention relates to an enzyme sensing determination method for indoleacetic acid content in plant sample, which includes the following steps: (1) enzyme sensor building: a platinum electrode is sequentially rubbed and polished by metallographic abrasive paper and muntjac skin with alumina suspension solution to form mirror surface, and the cleaned platinum electrode is performed with thionine electropolymerization and glutaraldehyde covalent cross-linking oxidase; (2) standard curve drawing: a series of IAA with different concentrations can be determined by the enzyme sensor, and the standard curve is drawn according to the relationship of current change and IAA concentrations; (3) IAA content detection of sample: the enzyme sensor is adopted to detect the current change of the sample, and the IAA concentration is calculated according to the standard curve. The invention has the beneficial effects that: the enzyme sensing determination can quickly and accurately determine the IAA content of the plant sample, compared with the traditional detection method, the enzyme sensing determination method is simple, obtains reliable results and has low determination cost.