The invention discloses a
dendrobium officinale protocorm-like body efficient proliferation culture medium and an application method thereof.The culture medium takes 1 / 2 MS as a basic culture medium, 30 g / L of mashed potatoes, 30 g / L of
sucrose, 7 g / L of
agar, 1 g / L of
activated carbon, 0.2 mg / L of
naphthylacetic acid (NAA) and 2 mg / L of 6-benzylamino adenine (6-BA) are added, pH is adjusted to 5.5-6.0, and preparation of the
dendrobium officinale protocorm-like body efficient proliferation culture medium is completed through
mother liquor preparation, ingredient mixing, pH adjustment and 121 DEG C high-pressure
steam sterilization for 30 min. During application, the protocorm-like body needs to be inoculated into the culture medium through high-pressure
steam sterilization, ultra-clean bench preparation, standardized split charging and sterile
inoculation operation, and then is cultured under the conditions of (27 + / -2) DEG C, 12h / d illumination and 4000-5000lx. According to the method, the proliferation coefficient of the protocorm-like body cultured for 30 days is not lower than 5.0, the
survival rate is not lower than 95%,
browning is avoided, large-scale proliferation of the
dendrobium officinale protocorm-like body can be efficiently achieved, and
technical support is provided for follow-up research and production.