1152results about How to "High reproductive coefficient" patented technology

The invention discloses a special medium for tissue culture of strawberry stem tips, belonging to the technical field of tissue culture seedling, comprising a first generation medium, a successive medium and a rooting medium, wherein the first generation medium is prepared by adding 6-benzyladenine with the concentration of 0.2-0.5mg/L and naphthylacetic acid with the concentration of 0.1-0.2mg/L to an MS medium; the successive medium is prepared by adding 6-benzyladenine with the concentration of 0.2-0.5mg/L to an MS medium; and the rooting medium is a 1/2 MS medium. The invention also discloses a method for producing detoxification seedlings by using strawberry stem tip tissues cultured by the mediums, comprising the steps of heat treatment of strawberry seedlings, material drawing and sterilization, first generation culture, successive culture, rooting culture, transplanting and the like. The strawberry detoxification fast propagation system of the invention is convenient and practical, and has good detoxification effect, high propagation factor, good genetic stability and high regeneration rate, thus being massively used on commercial purpose production.

The invention relates to a high-efficiency breeding method of healthy seedlings of cane, the method is proposed aiming at the problems that seed performance degenerates, yield is greatly reduced and the quality is reduced, which are caused by that the cane is taken as asexually propagated crop, propagation coefficient is low, the amount of seeds used for cane implantation is large, and after the implantation of a plurality of years, the cane is easy to be infected with a disease; improved type healthy seedlings of the cane can be cultured by nine steps of seedling mild water detoxication processing, seedling sprouting, in vitro peeling inoculation, induction culture, virus check, propagation culture, rootage culture, domesticated seedling-refining and outdoor temporary planting; the method adopts the combination of mild water detoxication and stem tip detoxication with thorough detoxication and good effect; the direct peeling inoculation of the explant does not need to be treated with disinfection processing, has low pollution rate and light browning, and is easy to generate clustering buds by induction; the propagation coefficient is high, the speed is high, the period is short; the seed performance is table, the seedling is strong; the virus detection is precise and accurate; the survival rate of the outdoor temporary planting is high and is up to more than 90 percent; the production cost is low, the efficiency is high, and the method is suitable for industrialized production.

The invention discloses a fast efficient propagation method of Paris polyphylla. The fast efficient propagation method of Paris polyphylla includes: selecting and treating propagating materials; inducing latent buds to germinate by stratification; and managing seedling propagation and production. The fast efficient propagation method of Paris polyphylla has the advantages that seedling growth is fast, propagation coefficient is high, and seedling quality is high. The latent buds are induced to germinate by inhibiting apical dominance without damaging completeness of rhizomes, stem cutting for seedling separation is performed according to germinating latent bud spots, and accordingly many latent buds germinate, and many separate seedlings are grown. Seedling rate is one to two times higher than that in the traditional way that segmental cutting is performed prior to inducing the latent buds to germinate. When the latent buds are induced to germinate, the rhizomes are not damaged. Therefore, seedlings from the latent buds are robust. By sterilization and seed soaking prior to seedling raising after cutting and seedling separation, strong seedlings are easy to obtain and survive easily after planted in fields.

The invention provides a rapid rhizoma polygonati propagation technology method. The method comprises the following steps: breaking seed dormancy by changing temperature treatment and hormone treatment, performing induction in vitro to culture callus by utilizing seeds subjected to dormancy breakage as materials, performing differentiation culture and root induction, and performing vegetative propagation on rhizoma polygonati seedlings. By adoption of the technology, the rhizoma polygonati propagation factor can be greatly improved, the production period is shortened, the sowing quantity is reduced, and the production cost is reduced.

The invention is a virus removal and rapid propagation technology of sweet potato variety 'Shangshu 19', comprising the following steps: selecting plants which are robust without diseases and insect pests and have characteristics of the variety from growing plants; clipping stem with stem tip; removing visible leaves; sterilizing; peeling the stem tip with 1-2 leaf primodia via a dissecting needle; quickly inoculating into a differentiation and induction culture medium; trimming the sterilized seedling into stem segments with 1-2 internode(s) and switching into an enrichment medium after virus detection; selecting sterilized seedling in vigorous growth to cut into small segments with 1-2 internode(s) and switch into a rooting medium; carrying out domestication on seedling after rooting. The invention has stem tip in virus removal and stem segment in rapid propagation, solves the problems of severe virus disease for sweet potato, poor quality of new cultured breed, germplasm degradation, low yield and the like propagated by using sweet potato block and stem. The invention can artificially control the growing conditions, can not be influenced by nature, and has the advantages of small amount of material use, economical culturing materials, short growth cycle, large propagation coefficient and convenient management and is favor of industrial production.

The invention discloses a seedling grafting breeding method. The seedling grafting breeding method comprises step 1, selecting root stock, selecting a scion of a seedling to be grafted and grafting the scion on the root stock; step 2, transplanting and planting the scion after the scion grows into a finished product seedling, performing deep planting on the finished product seedling during the planting and completely covering the combination position of the scion and the root stock with soil; step 3, cutting off the root stock after the combination position of the scion and the root stock roots and only reserving the scion and a self-root on the scion. The seedling which is bred by the seedling grafting breeding method has the self root and is free of the root stock and accordingly the self-root seedling can be bred rapidly and the breeding coefficient is improved.

The invention relates to a method of cuttage propagation for short spikes of a tea plant, which can prevent cuttage seedling from being damaged by drought and frost in the winter, and increase the survival rate and the rate of outplanting. The method comprises trimming nursery lands, cutting the short spikes, watering, covering the lands with film and uncovering the film in the spring. As a preferable scheme, the method requires fully watering after cutting the short spikes, spraying with fine water fog, uniformly and deeply watering until rooting holes are close and the short spikes do not fall down. Regularly, trimming nursery lands is carried out during the period from the later September to the middle October The invention has the following advantages: (1) because the short spikes are fully watered at a time and covered with mulches and grass during cottage period, the temperature and the humidity are kept; it is unnecessary to water in the whole winter; procedures are reduced; and production cost is decreased, (2) the root systems of the tea seedlings are good; the tea seedlings grow fast; the survival rate of nursery stock can reach over 90%; and the rate of outplanting can reach 80%; (3) rooting the short spikes which is postponed to the period from the later September to the middle October is beneficial to adjusting farming season.

A detoxicating fast reproduction method of strawberry by anther culture includes such steps as taking the flower bud of strong strawberry, storing at 4 deg.C in refrigerator for 72 hr, disinfecting with the mixture of alcohol, corrosive sublimate and tween, sucking surface water by aseptic suction paper, taking anther, and sequentially culturing in colli inducing culture medium, regrowing culture medium, secondary culture medium, and rooting culture medium.

The invention relates to a Taxus media cuttage method. The Taxus media cottage method is characterized by comprising the following processing steps that a place which is flat, sheltered, sufficient in water source, little in light and convenient to manage is selected to serve as a cuttage land; a cuttage bed is built by bricks on the periphery of the cuttage land, the cuttage bed is higher than the ground, and cuttage ground substances in the cuttage bed are formed by mixing perlite and turfy soil; half-lignification branches which grow in the current year and are on seed trees are collected and manufactured into cuttings, wherein the seed trees are free of plant diseases and insect pests and grow strongly, and complete sections with leaf branches are reserved at the upper portions of the cuttings; the cuttings are inserted into the cuttage ground substances in the density of 5*6cm, the insertion depth is 1/3-1/2 of the length of the cuttings, the cuttings make close contact with the cuttage ground substances, one cutting is watered every time the cutting is inserted, and a plastic arch shed covers the cuttings to shade the cuttings; strict control over moisture management, air humidity management, temperature management and seedling stage management is carried out after cuttage of the cuttings is finished. The rate of cuttage survival can reach 90%.

The invention belongs to the technical field of vegetative propagation of plants, in particular to a beach plum root cutting seeding method. By applying the invention, the problems of callus decay of a beach plum cuttage shoot root, low rooting rate, low survival rate and tender seeding which is easy to lose water and then withers are solved. Acclimatization in the process strengthens the growth and development of roots, stems and leaves and rejuvenation in the beach plum cuttage seeding process, remarkably improves the survival rate of field planting and establishes the firm foundation for the cultivation and generalization of the beach plum seeding which is a unique forest fruit.

The invention provides a method for rapidly propagating big camphor trees by layering high stems, which relates to a plant propagation method, in particular to a propagation method for camphor trees. The method comprises: a, choosing a seedling female parent; b, choosing a propagation cultivation place; c, choosing propagation time; d, choosing a good female parent and annularly cutting a gap which is 5 to 10 centimeters in a position 50 centimeters from ground; e, smearing disinfection solution and rooting liquid on bark in an annularly cut part, wrapping the gap in 1 to 5 kilograms of filling matrix, binding the annularly cut part tight with plastic cloth and packaging ropes and wrapping up to form a drum-shaped bag; and f, performing planting until white roots grow two months after annular cutting, turn yellow after eight months and grow to fill in the bag. Big trees produced by the propagation method can have two-thirds time of growth cycle shortened compared with normally sown and cultivated seedlings, and are developed in root systems, exuberant in growth vigor and capable of repeated regeneration and utilization.

The invention provides a new technology of inducing clustered shoots from parispolyphylla rhizome for rapid vegetative propagation. The new technology comprises: selecting a parispolyphylla rhizome to keep in reserve, drying in air for 3-7 days, making incisions on the back of the rhizome by using a cutter, immersing the incisions in an ABT rooting powder solution with a concentration of 10-30 ppm fro 2-10 h, disinfecting the incisions by plant ash, coating the parispolyphylla rhizome with a mixture of plant ash and clean river sands, transplanting and cultivating the parispolyphylla rhizome according to strains, coating fine soil with thickness of 5 mm on the parispolyphylla rhizome. After planting the rhizome, cluster buds germinate from stock plants and grow for about one year, then each parispolyphylla stock plant generates about more than twenty plantlets each of which possesses buds, small rhizome and a fine root, and the plantlets are taken as parispolyphylla seedlings for transplanting, and after 3-4 years, parispolyphylla finished products are obtained. The new technology has high propagation coefficient, and the produced parispolyphylla plantlets have high survival rate and fast growth speed. Compared with parispolyphylla seed propagation, the new technology helps to shorten more than one third of growth time of a finished product; and compared with a parispolyphylla rhizome fragment propagation method, the new technology helps to improve propagation coefficient by 4-6 times, and helps to solve the raw material gap facing medicinal materials and industrial production.

The invention relates to a tissue culture, rapid seedling formation and ecological application method for Louisiana iris, which comprises the following steps: 1) material selection: flowers, flower buds, floral axis, blades, roots and other vegetative organs of Louisiana iris are selected as explants; inoculation is carried out after routine disinfection; 2) bud induction and proliferation culture: the sterilized vegetative organs in 1) are inoculated on an induction medium; when callus has an area of 1 to 2 cm2, and forms a 0.5 to 0.8cm green convex block, the vegetative organs are transplanted in a differentiation medium; when the length of the vegetative organs reaches 5 to 8cm, the vegetative organs are transplanted in a rooting medium; 3) root induction: rootless seedlings with consistent growth are transplanted to a root induction medium; when the length of the rootless seedlings reaches 12 to 15cm, seedling adaptation is performed; 4) seedling adaptation: seedling adaptation is carried out for 6 to 8 days under natural light, then seedling emergence can be realized; culture medium at the root section of the seedlings is washed off; the seedlings are transplanted to a sterilized seedling container equipped with mixed substrate; after watering, shading and heat insulation, seedling emergence can be realized. The method has the advantages of fast seedling formation and good quality, and the seedlings can be directly applied to ecology.

The invention relates to a method for inducing an embryonal-suspensor mass (ESM) regeneration plant from an immature seed of pinus massoniana. By using the characteristic of continuous schizogenesis capability of the ESM in the immature seed of the pinus massoniana, solid culture and liquid culture are performed alternatively and ESM forms a completely-grown embryo with a normal structure after a maintaining stage, a multiplication stage, an embryo pre-mature stage and a maturation culture stage, so that a pinus massoniana plant is obtained by germinating and culturing the embryo. Due to the adoption of the method, a large number of regeneration plants of a pinus massoniana embryo of which the hereditary basis is accordant can be obtained and new technology with a short period and high occurring efficiency is provided for the storage of pinus massoniana rare generic resource and the mass production of excellent-gene nursery-grown plants.

The invention discloses a cutting and seedling method for phoebe shearer. The cutting and seedling method includes the steps: treating a cutting bed, namely paving cobblestones under a cutting bed and a layer of screen on the cutting bed and paving clean river sand for sterilization and disinfection of soil; collecting and producing cutting slips, namely clipping semi-lignification branches which are normal in growth with full shoots and without diseases and insect pests as cuttings from a phoebe shearer seed tree of 5-8 years from March to April to produce cutting slips; subjecting the produced cutting slips to early stage treatment and cutting; performing management after cutting. The cutting and seedling method is simple in operation, high in survival rate and capable of providing fine phoebe shearer seedlings, shortening breeding time of fine seeds and protecting excellent genetic traits of the seedlings. Therefore, propagation coefficient is greatly increased, and the survival rate reaches 80% at least.

The invention discloses a cottage breeding method of sabina przewalskii, comprising the following steps: manufacture of a cottage seedling bed: manufacturing a seedling bed on the seedling culturing ground in a greenhouse in the spring of the current year; and covering fine sand and mycorrhiza soil as cottage matrix; strip picking: in the middle of May to June, selecting healthy, strong, pest and disease-and-damage-free sabina przewalskiiseedlings at the age of 3-5 with good growth and development for picking strips; and cutting processing: placing the cutting into clear water for soaking, drying rapidly, respectively placing into ABT1 rooting powder and potassium permanganate solution for respectively soaking for one time and then carrying out cottage; digging holes on the cottage seedling bed and obliquely cutting, and compacting the cutting hole; leading the cutting bed and the cutting to be fully contacted; spraying with carbendazim or Tuzet and copper abietate for once every half a month, thus preventing root rot of sabina przewalskii seedlings. The method of the invention greatly improves propagation coefficient, can shorten seeding culturing period, has high cottage seedling culturing survival rate, and can enable the cultured nursery stock to keep excellent characters of parent trees.

A tissue culturing method for quercus virginiana belongs to plant tissue culturing methods and is characterized by comprising the following steps: 1 selecting of explants; 2 disinfection of the explants; 3 primary culture; 4 multiplication culturing of adventitious buds; 5 culturing of strong seedlings; 6 culturing of strike roots; and 7 seedling acclimatization and transplanting. The tissue culturing method for the quercus virginiana can keep stress resistance and enjoyment of a female parent and can effectively increase propagation coefficient of the quercus virginiana. Compared with seminal propagation, tissue culturing reduces dependency on imported seeds, can effectively reduce nursery stock cost, and saves expenditures. Compared with cutting propagation, the tissue culturing is short in seedling culturing period, can obtain the nursery stock with strike roots within 4 months, and saves cost.

The invention relates to a generative propagation breeding technology of dendrocalamus giganteus munro, comprising the following steps: seed collection and selection, seed storage, seed sowing and culture of seedling, preparation before planting bag seedling, planting, cultivation and protection, selective breeding of bamboo seedling, pest control, seed selection and survey of growth of the dendrocalamus giganteus munro. The sowing and the breeding of the invention has the characteristics of high reproduction coefficient, strong adaptability, strong stress resistance, ordered forest form, long life cycle, low afforestation expense, small labor intensity and the like.