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207results about How to "High reproductive coefficient" patented technology

Efficient breeding method of sugarcane health seedling

The invention relates to a high-efficiency breeding method of healthy seedlings of cane, the method is proposed aiming at the problems that seed performance degenerates, yield is greatly reduced and the quality is reduced, which are caused by that the cane is taken as asexually propagated crop, propagation coefficient is low, the amount of seeds used for cane implantation is large, and after the implantation of a plurality of years, the cane is easy to be infected with a disease; improved type healthy seedlings of the cane can be cultured by nine steps of seedling mild water detoxication processing, seedling sprouting, in vitro peeling inoculation, induction culture, virus check, propagation culture, rootage culture, domesticated seedling-refining and outdoor temporary planting; the method adopts the combination of mild water detoxication and stem tip detoxication with thorough detoxication and good effect; the direct peeling inoculation of the explant does not need to be treated with disinfection processing, has low pollution rate and light browning, and is easy to generate clustering buds by induction; the propagation coefficient is high, the speed is high, the period is short; the seed performance is table, the seedling is strong; the virus detection is precise and accurate; the survival rate of the outdoor temporary planting is high and is up to more than 90 percent; the production cost is low, the efficiency is high, and the method is suitable for industrialized production.

Virus removal and rapid propagation technology of sweet potato variety 'Shangshu 19' and culture material

InactiveCN101611697AImprove immunitySolve the serious sweet potato virus diseaseCultivating equipmentsPlant tissue cultureDiseaseGermplasm
The invention is a virus removal and rapid propagation technology of sweet potato variety 'Shangshu 19', comprising the following steps: selecting plants which are robust without diseases and insect pests and have characteristics of the variety from growing plants; clipping stem with stem tip; removing visible leaves; sterilizing; peeling the stem tip with 1-2 leaf primodia via a dissecting needle; quickly inoculating into a differentiation and induction culture medium; trimming the sterilized seedling into stem segments with 1-2 internode(s) and switching into an enrichment medium after virus detection; selecting sterilized seedling in vigorous growth to cut into small segments with 1-2 internode(s) and switch into a rooting medium; carrying out domestication on seedling after rooting. The invention has stem tip in virus removal and stem segment in rapid propagation, solves the problems of severe virus disease for sweet potato, poor quality of new cultured breed, germplasm degradation, low yield and the like propagated by using sweet potato block and stem. The invention can artificially control the growing conditions, can not be influenced by nature, and has the advantages of small amount of material use, economical culturing materials, short growth cycle, large propagation coefficient and convenient management and is favor of industrial production.

Single-node cutting seed-raising method of tea plant

The invention relates to a method of cuttage propagation for short spikes of a tea plant, which can prevent cuttage seedling from being damaged by drought and frost in the winter, and increase the survival rate and the rate of outplanting. The method comprises trimming nursery lands, cutting the short spikes, watering, covering the lands with film and uncovering the film in the spring. As a preferable scheme, the method requires fully watering after cutting the short spikes, spraying with fine water fog, uniformly and deeply watering until rooting holes are close and the short spikes do not fall down. Regularly, trimming nursery lands is carried out during the period from the later September to the middle October The invention has the following advantages: (1) because the short spikes are fully watered at a time and covered with mulches and grass during cottage period, the temperature and the humidity are kept; it is unnecessary to water in the whole winter; procedures are reduced; and production cost is decreased, (2) the root systems of the tea seedlings are good; the tea seedlings grow fast; the survival rate of nursery stock can reach over 90%; and the rate of outplanting can reach 80%; (3) rooting the short spikes which is postponed to the period from the later September to the middle October is beneficial to adjusting farming season.

Taxus media cuttage method

The invention relates to a Taxus media cuttage method. The Taxus media cottage method is characterized by comprising the following processing steps that a place which is flat, sheltered, sufficient in water source, little in light and convenient to manage is selected to serve as a cuttage land; a cuttage bed is built by bricks on the periphery of the cuttage land, the cuttage bed is higher than the ground, and cuttage ground substances in the cuttage bed are formed by mixing perlite and turfy soil; half-lignification branches which grow in the current year and are on seed trees are collected and manufactured into cuttings, wherein the seed trees are free of plant diseases and insect pests and grow strongly, and complete sections with leaf branches are reserved at the upper portions of the cuttings; the cuttings are inserted into the cuttage ground substances in the density of 5*6cm, the insertion depth is 1/3-1/2 of the length of the cuttings, the cuttings make close contact with the cuttage ground substances, one cutting is watered every time the cutting is inserted, and a plastic arch shed covers the cuttings to shade the cuttings; strict control over moisture management, air humidity management, temperature management and seedling stage management is carried out after cuttage of the cuttings is finished. The rate of cuttage survival can reach 90%.

New technology of inducing clustered shoots from parispolyphylla rhizome for rapid vegetative propagation

The invention provides a new technology of inducing clustered shoots from parispolyphylla rhizome for rapid vegetative propagation. The new technology comprises: selecting a parispolyphylla rhizome to keep in reserve, drying in air for 3-7 days, making incisions on the back of the rhizome by using a cutter, immersing the incisions in an ABT rooting powder solution with a concentration of 10-30 ppm fro 2-10 h, disinfecting the incisions by plant ash, coating the parispolyphylla rhizome with a mixture of plant ash and clean river sands, transplanting and cultivating the parispolyphylla rhizome according to strains, coating fine soil with thickness of 5 mm on the parispolyphylla rhizome. After planting the rhizome, cluster buds germinate from stock plants and grow for about one year, then each parispolyphylla stock plant generates about more than twenty plantlets each of which possesses buds, small rhizome and a fine root, and the plantlets are taken as parispolyphylla seedlings for transplanting, and after 3-4 years, parispolyphylla finished products are obtained. The new technology has high propagation coefficient, and the produced parispolyphylla plantlets have high survival rate and fast growth speed. Compared with parispolyphylla seed propagation, the new technology helps to shorten more than one third of growth time of a finished product; and compared with a parispolyphylla rhizome fragment propagation method, the new technology helps to improve propagation coefficient by 4-6 times, and helps to solve the raw material gap facing medicinal materials and industrial production.

Louis iris tissue culture fast seedling establishment and ecological application method

The invention relates to a tissue culture, rapid seedling formation and ecological application method for Louisiana iris, which comprises the following steps: 1) material selection: flowers, flower buds, floral axis, blades, roots and other vegetative organs of Louisiana iris are selected as explants; inoculation is carried out after routine disinfection; 2) bud induction and proliferation culture: the sterilized vegetative organs in 1) are inoculated on an induction medium; when callus has an area of 1 to 2 cm2, and forms a 0.5 to 0.8cm green convex block, the vegetative organs are transplanted in a differentiation medium; when the length of the vegetative organs reaches 5 to 8cm, the vegetative organs are transplanted in a rooting medium; 3) root induction: rootless seedlings with consistent growth are transplanted to a root induction medium; when the length of the rootless seedlings reaches 12 to 15cm, seedling adaptation is performed; 4) seedling adaptation: seedling adaptation is carried out for 6 to 8 days under natural light, then seedling emergence can be realized; culture medium at the root section of the seedlings is washed off; the seedlings are transplanted to a sterilized seedling container equipped with mixed substrate; after watering, shading and heat insulation, seedling emergence can be realized. The method has the advantages of fast seedling formation and good quality, and the seedlings can be directly applied to ecology.

Tissue-culture seedling raising method of rhodiola crenulata

The invention provides a tissue-culture seedling raising method of rhodiola crenulata, which comprises the following steps of: (1) taking the leaves of the rhodiola crenulata as an explant and carrying out budding culture to form adventitious buds; (2) carrying out propagation culture on the adventitious buds to form single seedlings; (3) carrying out rootage culture on the single seedlings obtained after stretching cluster buds to form rootage seedlings; and (4) transplanting the rootage seedlings, wherein a culture medium for the budding culture is an MS culture medium added with 10 to 20mumol/L of 6-benzylaminopurine and 1 to 5mumol/L of gibberellin. The invention reinforces the high-efficiency seedling raising method of the rhodiola crenulata by primary culture dark processing and the combined processing of the addition of active carbon in the rootage process and solves the problem of low propagation coefficient of the rootage seedlings of the rhodiola crenulata, the multiplication coefficient of 20 days of the cluster buds is 2 to 3 times, the rootage rate reaches more than 95 percent, and the transplanting survival rate is as high as more than 90 percent. The method provided by the invention has high differentiation frequency and short growth cycle and is easy for the large-scale production of the rhodiola crenulata.

Asexual propagation seedling method of hybrid paper mulberry

The invention discloses a tissue culture rapid propagation seedling method of hybrid paper mulberry, and belongs to the technical field of hybrid paper mulberry plantation. The tissue culture rapid propagation seedling method of hybrid paper mulberry comprises following steps: explant selection, induced culturing, subculturing, rooting culture, and transplanting. According to the tissue culture rapid propagation seedling method, current year stems with buds are selected as explants, the meristematic capacity is excellent, and differentiation propagation is more convenient to realize; LED illumination culture is capable of adjusting culture material growth process, increasing the environmental adaptability, realizing reasonable optimization on composition of induction medium, proliferationmedium, and rooting medium, and illumination conditions of different stages, increasing seedling tissue culture efficiency, and achieving relatively induction rate, reproduction rate, and rooting rate; the adventitious bud induction differentiation rate is 94% or higher; after subculture, average seedling height reaches 3.5cm, rooting rate reaches 100%, and transplanting survival rate reaches 68%or higher. The tissue culture rapid propagation seedling method is adopted for seedling of hybrid paper mulberry, the seedling efficiency is high, the propagation coefficient is large, seedling cost is reduced, and the obtained tissue culture seedlings are strong, and are high in quality.

Barbadosnut plantlet tissue culture rapid propagation and rooting method

The invention discloses a barbadosnut plantlet tissue culture rapid propagation and rooting method, which comprises the steps as follows: a germ of a barbadosnut seed after disinfection and sterilization is placed in a basic culture medium with the pH value of 5.4 to 6.0, and cultured to germinate into a sterile plantlet after 5 days at the culture temperature of 25 to 35 DEG C, with the illumination intensity of 1500 to 3000lx and the illumination time of 12h/d; stem segments with single bud of the sterile plantlet are placed in the rapid propagation culture medium, and cultured for 7days so as to induce out cluster buds; and the cluster buds growing to 2 to 3cm are cut and inoculate to a rooting culture medium, start to root after being cultured for another 5 days, and then grow into 2 to 3 cm strong stocks after 15 days, thus being capable of being transplanted. Only 66 days are needed for the rooting of the first propagation plantlet from the seed germination, therefore, the propagation is rapid; the hormone concentration of the culture process is low, the contact time is short, and the variation probability is low; 4.25 plantets can be obtained by utilizing one seed for 3 weeks, therefore, the propagation coefficient is high; and the types of used agents are single, the usage amount is small, the price is cheap and the cost is low.

Apple dwarf self-rooted rootstock big seedling scionstock integrated breeding method

The invention discloses an apple dwarf self-rooted rootstock big seedling scionstock integrated breeding method The apple dwarf self-rooted rootstock big seedling scionstock integrated breeding method is specifically implemented in the way that during the breeding process, layering is carried out in advance to promote the generation of branches, the amount of reserved branches is three times that of original reserved branches after division of plants, layering is carried out in rows the next year, and the propagation area and propagation coefficient are increased; at the same time, grafting is carried out in the stock growing season, the aim that breeding, rooting and scion grafting of the dwarf stock are carried out in current year, scions germinate in the current year, and sprout and generate branches, the grafted seedlings can live through the winter normally is achieved, after the grafted seedlings are separated from the stock mother plant and are planted to the field, and after an annual growth season, the grafted seedlings generate branches to form big seedlings with multiple branches. Thus, compared with a conventional seedling growing mode, the apple dwarf self-rooted rootstock big seedling scionstock integrated breeding method is advantaged in that the quality bred seedlings can be greatly improved in unit time and the quantity of bred seedlings can be greatly increased in unit time.

Tissue culture propagation method for reducing tissue culture browning rate of traxacum koksaghyz

The invention belongs to the field of plant tissue culture of plant cell engineering techniques, and in particular relates to a tissue culture propagation method for reducing the tissue culture browning rate of traxacum koksaghyz. The tissue culture propagation method comprises the following steps: inoculating, namely, selecting mature seeds of a traxacum koksaghyz strain; performing induced differentiation on calluses; inducing callus formation; proliferating adventitious buds; performing rooting culture; separating multiple shoots into single plants, and transferring to a rooting culture medium for rooting culture; performing acclimatization and transplantation, thereby obtaining regenerated plants of traxacum koksaghyz. By adopting the tissue culture propagation method, a method for effectively reducing the tissue culture browning rate of traxacum koksaghyz and rapidly propagating traxacum koksaghyz is provided, the tissue culture browning rate of traxacum koksaghyz is greatly reduced through technical measures such as adjusting components of culture mediums used in different stages and adding an antioxidant, the propagation coefficient of traxacum koksaghyz is improved, the browning rate can be reduced to be less than 35% when the culture mediums provided by the invention is adopted, and the browning rate can be further reduced to be less than 10% when Na2S2O3 is added.

Method for plant leaf bud shoot cutting propagation all year around

ActiveCN103875435AThe promotion cycle is shortenedLarge amount of reproductionHorticultureShootCell budding
The invention relates to a method for plant leaf bud shoot cutting propagation all year around and relates to the technical field of vegetative propagation. The method aims to overcome the defects that in the prior art, nursery stock seedling propagation is large in variation and part of nursery stock is low in maturing rate, and tissue culture propagation is high in cost and difficult in technology management, and minimum stems of existing fine seed nursery stock leaf buds are adopted as female parent cuttage materials for cuttage. The method includes the steps of solar greenhouse or movable greenhouse facility and planting bed preparation, leaf bud selection, cuttage time and method determination and seedling period management. The propagation coefficient of seedlings cultivated in the method can be progressively increased in a multiplied mode, the survival rate of the seedlings can reach 90%, roots are developed and grow vigorously, and the transplantation survival rate can exceed 95%. The method has the greatest advantages that mass production and industrialized production can be achieved, propagation does not need professionals, and the method is easy to operate, low in individual cost, high in propagated seedling survival rate and easy to popularize.

Tamarix chinensis tissue culture and rapid propagation method

The invention relates to the technical field of wood tissue culture seedling raising in forest science, in particular to a Tamarix chinensis tissue culture and rapid propagation method. The method includes: acquisition of aseptic seedlings, with an involved medium containing 6-BA, IBA, GA, sucrose and agar; subculture: inoculating the aseptic seedlings into a subculture medium to conduct culture so as to obtain clustered shoots, with the medium containing ZT, IBA, sucrose and agar; rooting culture: inoculating the clustered shoots into a rooting medium containing IBA, NAA, GA, sucrose and agar to obtain rooted tissue cultured seedlings; and acclimatization and transplantation. The propagation method has high propagation coefficient and short propagation cycle, the obtained seedlings are strong and have a lot of root systems, and the survival rate of transplantation is over 96%. The method provided by the invention omits the strong seedling culture step in conventional methods, improves propagation efficiency, makes the Tamarix chinensis tissue culture and rapid propagation technology move towards production from laboratories, realizes large-scale production of Tamarix chinensis tissue culture seedlings, and provides technical support for promotion of Tamarix chinensis rapid propagation.
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