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1152results about How to "High reproductive coefficient" patented technology

Cuttage and breeding method for sabina vulgalis

A cuttage and breeding method for sabina vulgalis comprises the steps of renovating a cuttage bed, picking branches, processing shoot for cutting, performing cuttage and the like; deeply ploughing a seedling tray, clearing off impurities, applying cottage, leveling, widening to make a lower bed, and paving clean river sand on the seedling bed; sterilizing the sand bed which is 5-10cm thick with 800-1000 times of carbendazol liquid medicine; picking 1-2 years old healthy and strong branches without diseases or pests at dawn, putting the branches cut down in a plastic cask constraining water timely to prevent drying-up due to water loss; processing shoot for cutting by adopting different medicines and plant growing hormone with concentration being 50-150ppm for dipping for 2-6h, so that breeding coefficient is higher. Facts prove that the cuttage and breeding survival rate of sabina vulgalis bred by using the invention can reach up to 75-89%, thus realizing the aim of enhancing breeding coefficient.
Owner:GANSU PROVINCE ACAD OF QILIAN WATER RESOURCE CONSERVATION FORESTS RES INST

Special medium for tissue culture of strawberry stem tip and method thereof for producing detoxification seedlings

The invention discloses a special medium for tissue culture of strawberry stem tips, belonging to the technical field of tissue culture seedling, comprising a first generation medium, a successive medium and a rooting medium, wherein the first generation medium is prepared by adding 6-benzyladenine with the concentration of 0.2-0.5mg / L and naphthylacetic acid with the concentration of 0.1-0.2mg / L to an MS medium; the successive medium is prepared by adding 6-benzyladenine with the concentration of 0.2-0.5mg / L to an MS medium; and the rooting medium is a 1 / 2 MS medium. The invention also discloses a method for producing detoxification seedlings by using strawberry stem tip tissues cultured by the mediums, comprising the steps of heat treatment of strawberry seedlings, material drawing and sterilization, first generation culture, successive culture, rooting culture, transplanting and the like. The strawberry detoxification fast propagation system of the invention is convenient and practical, and has good detoxification effect, high propagation factor, good genetic stability and high regeneration rate, thus being massively used on commercial purpose production.
Owner:北京金六环农业科技有限公司

Efficient breeding method of sugarcane health seedling

The invention relates to a high-efficiency breeding method of healthy seedlings of cane, the method is proposed aiming at the problems that seed performance degenerates, yield is greatly reduced and the quality is reduced, which are caused by that the cane is taken as asexually propagated crop, propagation coefficient is low, the amount of seeds used for cane implantation is large, and after the implantation of a plurality of years, the cane is easy to be infected with a disease; improved type healthy seedlings of the cane can be cultured by nine steps of seedling mild water detoxication processing, seedling sprouting, in vitro peeling inoculation, induction culture, virus check, propagation culture, rootage culture, domesticated seedling-refining and outdoor temporary planting; the method adopts the combination of mild water detoxication and stem tip detoxication with thorough detoxication and good effect; the direct peeling inoculation of the explant does not need to be treated with disinfection processing, has low pollution rate and light browning, and is easy to generate clustering buds by induction; the propagation coefficient is high, the speed is high, the period is short; the seed performance is table, the seedling is strong; the virus detection is precise and accurate; the survival rate of the outdoor temporary planting is high and is up to more than 90 percent; the production cost is low, the efficiency is high, and the method is suitable for industrialized production.
Owner:SUGARCANE RES INST OF YUNNAN ACADEMY OF AGRI SCI

Method for reconstructing and recovering wetland vegetation by transplanting reed roots

The invention belongs to a wetland vegetation modification method, and particularly relates to a method for reconstructing and recovering reed vegetation by transplanting reed roots. Simple steps and methods of transplanting reed roots, digging and collecting reed roots, leveling transplantation soil, transmitting and storing roots in moisture, cutting shorter root lengths in root transplantationaccording to the self bionomical characteristics of reed, fully burying the root segments underground for trench plantation and hole plantation, keeping the moisture state of the soil from the plantation to the emergence of seedlings, growing the reed under natural conditions are used to save root seedlings, improve the root propagation coefficient, save energy consumption and improve the work efficiency so as to achieve the aims of high-efficiency, rapid and asexual reconstruction, reed vegetation recovery and acquire favorable economic, social and biological benefits.
Owner:NORTHEAST NORMAL UNIVERSITY

Fast efficient propagation method of Paris polyphylla

InactiveCN102870564ASeedling speed is fastMore germinationHorticultureBudRhizome
The invention discloses a fast efficient propagation method of Paris polyphylla. The fast efficient propagation method of Paris polyphylla includes: selecting and treating propagating materials; inducing latent buds to germinate by stratification; and managing seedling propagation and production. The fast efficient propagation method of Paris polyphylla has the advantages that seedling growth is fast, propagation coefficient is high, and seedling quality is high. The latent buds are induced to germinate by inhibiting apical dominance without damaging completeness of rhizomes, stem cutting for seedling separation is performed according to germinating latent bud spots, and accordingly many latent buds germinate, and many separate seedlings are grown. Seedling rate is one to two times higher than that in the traditional way that segmental cutting is performed prior to inducing the latent buds to germinate. When the latent buds are induced to germinate, the rhizomes are not damaged. Therefore, seedlings from the latent buds are robust. By sterilization and seed soaking prior to seedling raising after cutting and seedling separation, strong seedlings are easy to obtain and survive easily after planted in fields.
Owner:临沧道地中药材种植科技有限公司

Tissue Culture Rapid Propagation Method of Ginger Lotus

The invention discloses a rapid tissue culture and propagation method of curcuma alsimatifolia, belonging to the technical field of plant tissue culture. The method comprises the following steps of: firstly, preparation of a culture medium; secondly, culture and sterilization of earthnut budlet; thirdly, culture of explant sterile seedling; fourthly, induction culture of adventitious bud; fifthly, propagation culture of adventitious bud; sixthly, root growing culture of plantlet; and seventhly, seedling adaptation, acclimation and transplanting of tissue culture seedling. The method disclosedby the invention has the advantages that propagation culture period is short (14-20 days), propagation coefficient is high (improved to 5-8 times from 2-4 times), the consistency of the adventitious bud is good, tissue culture breeding cycle is short (50-60 days), seedling quality is good, and transplanting survival rate can reach up to more than 95%, and is a method for efficiently and rapidly providing high-quality curcuma alsimatifolia seedlings. The method disclosed by the invention can be popularized and applied to landscaping and tissue culture seedling enterprises.
Owner:ZHEJIANG XIAOSHAN COTTON & FLAX RES INST

Rapid rhizoma polygonati propagation technology method

The invention provides a rapid rhizoma polygonati propagation technology method. The method comprises the following steps: breaking seed dormancy by changing temperature treatment and hormone treatment, performing induction in vitro to culture callus by utilizing seeds subjected to dormancy breakage as materials, performing differentiation culture and root induction, and performing vegetative propagation on rhizoma polygonati seedlings. By adoption of the technology, the rhizoma polygonati propagation factor can be greatly improved, the production period is shortened, the sowing quantity is reduced, and the production cost is reduced.
Owner:湖南宏邦生物科技有限公司

Virus removal and rapid propagation technology of sweet potato variety 'Shangshu 19' and culture material

InactiveCN101611697AImprove immunitySolve the serious sweet potato virus diseaseCultivating equipmentsPlant tissue cultureDiseaseGermplasm
The invention is a virus removal and rapid propagation technology of sweet potato variety 'Shangshu 19', comprising the following steps: selecting plants which are robust without diseases and insect pests and have characteristics of the variety from growing plants; clipping stem with stem tip; removing visible leaves; sterilizing; peeling the stem tip with 1-2 leaf primodia via a dissecting needle; quickly inoculating into a differentiation and induction culture medium; trimming the sterilized seedling into stem segments with 1-2 internode(s) and switching into an enrichment medium after virus detection; selecting sterilized seedling in vigorous growth to cut into small segments with 1-2 internode(s) and switch into a rooting medium; carrying out domestication on seedling after rooting. The invention has stem tip in virus removal and stem segment in rapid propagation, solves the problems of severe virus disease for sweet potato, poor quality of new cultured breed, germplasm degradation, low yield and the like propagated by using sweet potato block and stem. The invention can artificially control the growing conditions, can not be influenced by nature, and has the advantages of small amount of material use, economical culturing materials, short growth cycle, large propagation coefficient and convenient management and is favor of industrial production.
Owner:周玉玲

Seedling grafting breeding method

The invention discloses a seedling grafting breeding method. The seedling grafting breeding method comprises step 1, selecting root stock, selecting a scion of a seedling to be grafted and grafting the scion on the root stock; step 2, transplanting and planting the scion after the scion grows into a finished product seedling, performing deep planting on the finished product seedling during the planting and completely covering the combination position of the scion and the root stock with soil; step 3, cutting off the root stock after the combination position of the scion and the root stock roots and only reserving the scion and a self-root on the scion. The seedling which is bred by the seedling grafting breeding method has the self root and is free of the root stock and accordingly the self-root seedling can be bred rapidly and the breeding coefficient is improved.
Owner:刁久新

Method for rapidly propagating taxus chinensis seedlings

InactiveCN102726202ASolve the contradiction between supply and demand of resourcesEasy to operateGreenhouse cultivationFertilizer mixturesNutrient solutionSeedling
The invention discloses a method for rapidly propagating taxus chinensis seedlings, and the method comprises the steps of preparing nutrient soil, nutrient solution, pretreatment solution and rooting solution by using a plant tissue culture principle; filling the nutrient soil into a nutrient bag; drenching the nutrient soil in the nutrient bag by using the nutrient solution; taking a taxus chinensis semi-lignified twig as a propagation material; shearing the taxus chinensis semi-lignified twig into bud-containing subsections which are 0.5-12cm long; inserting the bud-containing subsections into the nutrient bag after being treated with the pretreatment solution; one week later, spraying the rooting solution which is diluted by 1000-1200 times for promoting the rooting; and spraying once every week until the rooting is finished. Through culture and management for 3-4 weeks, the bud-containing subsection roots become a new plant, the survival rate is above 90% and the root system is well developed. The method provided by the invention has the following advantages of being simple for operation, easy for grasping, feasible in four seasons and beneficial for popularization, well solving the technical problem of rapidly propagation of taxus chinensis seedlings, realizing industrial seedling culture, and being conducive to protecting the precious wild taxus chinensis resources of China.
Owner:湖南省森林植物园

Method for breeding minitype potato seeds by directly using potato stem segments

The invention provides a production method of minitype potato seeds bred by directly using potato stem segments, relating to the minitype potato seed high power propagation technology. In the invention, potato detoxification test tube tissue culture plantlet stem segments are used as a raw material, and then are directly implanted in an MS liquid or solid induction medium without any plant growth hormone, and the minitype potato seeds capable of sprouting are induced and produced in 6-8 weeks. The tissue culture plantlet stem segments are directly bred without changing the medium, thus obtaining the potato seeds in one step. The method has the advantages of saving time, labor and cost, having simple equipment, less used plantlets, less consumed time, rapid propagation and high efficiency and the like. The breeding period is 1.5-2 months, production can be performed for 5-6 periods each year, and the breeding chamber at unit area can produce 500,000-600,000 minitype potato seeds, thus making full use of the tissue culture chamber production space, greatly improving the output of the detoxification minitype potato seeds at each unit area, and increasing the production efficiency at each unit area by 50-100 times. The method is suitable for large-scale factory production of minitype potato seeds.
Owner:LELING XISEN POTATO IND GROUP

Single-node cutting seed-raising method of tea plant

The invention relates to a method of cuttage propagation for short spikes of a tea plant, which can prevent cuttage seedling from being damaged by drought and frost in the winter, and increase the survival rate and the rate of outplanting. The method comprises trimming nursery lands, cutting the short spikes, watering, covering the lands with film and uncovering the film in the spring. As a preferable scheme, the method requires fully watering after cutting the short spikes, spraying with fine water fog, uniformly and deeply watering until rooting holes are close and the short spikes do not fall down. Regularly, trimming nursery lands is carried out during the period from the later September to the middle October The invention has the following advantages: (1) because the short spikes are fully watered at a time and covered with mulches and grass during cottage period, the temperature and the humidity are kept; it is unnecessary to water in the whole winter; procedures are reduced; and production cost is decreased, (2) the root systems of the tea seedlings are good; the tea seedlings grow fast; the survival rate of nursery stock can reach over 90%; and the rate of outplanting can reach 80%; (3) rooting the short spikes which is postponed to the period from the later September to the middle October is beneficial to adjusting farming season.
Owner:南京新世纪园艺研究所

Artificial rearing method of eurysome golden thread leech

InactiveCN1476753AMaintain morphological characteristicsBody sizeAnimal husbandryArtificial rearingZoology
The artificial breeding method of wide-body golden-thread leech includes the following steps: building artificial breeding pool of wide-body golden-thread leech, seed leech selection, culturing larvaand cultivation of the wide-body golden-thread leech in large pool. Its propagation coefficient is obviously raised and stable, and is above 1:80, generally. The breeding method only has requirementsfor 2-3 years.
Owner:NANJING AGRICULTURAL UNIVERSITY

Method for carrying out taking off poison and quick breeding by using strawberry anther

InactiveCN101049090AIncreased rate of culture differentiationBreak through the predicament of low traditional differentiation ratePlant tissue cultureHorticulture methodsAlcoholBud
A detoxicating fast reproduction method of strawberry by anther culture includes such steps as taking the flower bud of strong strawberry, storing at 4 deg.C in refrigerator for 72 hr, disinfecting with the mixture of alcohol, corrosive sublimate and tween, sucking surface water by aseptic suction paper, taking anther, and sequentially culturing in colli inducing culture medium, regrowing culture medium, secondary culture medium, and rooting culture medium.
Owner:NANJING AGRICULTURAL UNIVERSITY

Paris polyphylla var. pseudothibetica rhizome multiple shoot asexual reproduction method

The invention relates to a propagation method in the field of vegetative propagation, specially relates to a cluster bud propagation method of Paris polyphylla rhizomatic of Paridis. The invention is to provide a method with the advantages of simple operation, low cost, high propagation coefficient, rapid plumule growth, good seedling growth vigor, and no influence to the medicinal value of the propagating material to solve the problem of hard propagation of Paris polyphylla. The cluster bud propagation method of Paris polyphylla rhizom comprises selecting propagating material, processing method of the propagating material, processing time of the propagating material which can be performed all year around, inducing circumstance of cluster bud and managing, transplanting the seedling of cluster bud. Compared with the present technology, the invention has the advantages of simple operation, low cost, high propagation coefficient, good seedling growth vigor, and no influence to the medicinal value of the propagating material.
Owner:ENSHI JIYUAN PHARMA TECH DEV

Potato isolated culture one-step seedling culture medium and optimization method and seedling method thereof

The invention discloses a method for optimizing a potato isolated culture one-step seedling culture medium. The culture medium makes leaves of a potato test tube plantlet undergo isolated culture to form a seedling in one step. The one-step seedling culture medium takes an MS culture medium as a basal medium, and 6-benzyladenine, naphthyl acetic acid and 2,4-dichlorphenoxyacetic acid with different concentrations and combinations are supplied in 1L of the MS culture medium; adventitious buds and adventitious roots are directly differentiated through callus induction; and the potato regenerated seedling is obtained in one step. The callus obtained by induction in a primary culture medium is unnecessarily inoculated to a differential medium for regenerating the seedling. Compared with an isolated culture multi-step regenerated seedling method, the method of the invention has the advantages of simplified steps, short culture period, high repeatability, and high seedling survival rate.
Owner:SICHUAN AGRI UNIV

Taxus media cuttage method

The invention relates to a Taxus media cuttage method. The Taxus media cottage method is characterized by comprising the following processing steps that a place which is flat, sheltered, sufficient in water source, little in light and convenient to manage is selected to serve as a cuttage land; a cuttage bed is built by bricks on the periphery of the cuttage land, the cuttage bed is higher than the ground, and cuttage ground substances in the cuttage bed are formed by mixing perlite and turfy soil; half-lignification branches which grow in the current year and are on seed trees are collected and manufactured into cuttings, wherein the seed trees are free of plant diseases and insect pests and grow strongly, and complete sections with leaf branches are reserved at the upper portions of the cuttings; the cuttings are inserted into the cuttage ground substances in the density of 5*6cm, the insertion depth is 1 / 3-1 / 2 of the length of the cuttings, the cuttings make close contact with the cuttage ground substances, one cutting is watered every time the cutting is inserted, and a plastic arch shed covers the cuttings to shade the cuttings; strict control over moisture management, air humidity management, temperature management and seedling stage management is carried out after cuttage of the cuttings is finished. The rate of cuttage survival can reach 90%.
Owner:JIANGSU HONGDOUSHAN BIOLOGICAL TECH

Beach plum root cutting seeding method

The invention belongs to the technical field of vegetative propagation of plants, in particular to a beach plum root cutting seeding method. By applying the invention, the problems of callus decay of a beach plum cuttage shoot root, low rooting rate, low survival rate and tender seeding which is easy to lose water and then withers are solved. Acclimatization in the process strengthens the growth and development of roots, stems and leaves and rejuvenation in the beach plum cuttage seeding process, remarkably improves the survival rate of field planting and establishes the firm foundation for the cultivation and generalization of the beach plum seeding which is a unique forest fruit.
Owner:南京逐鹿景观工程有限公司

Method for rapidly propagating big camphor trees by layering high stems

The invention provides a method for rapidly propagating big camphor trees by layering high stems, which relates to a plant propagation method, in particular to a propagation method for camphor trees. The method comprises: a, choosing a seedling female parent; b, choosing a propagation cultivation place; c, choosing propagation time; d, choosing a good female parent and annularly cutting a gap which is 5 to 10 centimeters in a position 50 centimeters from ground; e, smearing disinfection solution and rooting liquid on bark in an annularly cut part, wrapping the gap in 1 to 5 kilograms of filling matrix, binding the annularly cut part tight with plastic cloth and packaging ropes and wrapping up to form a drum-shaped bag; and f, performing planting until white roots grow two months after annular cutting, turn yellow after eight months and grow to fill in the bag. Big trees produced by the propagation method can have two-thirds time of growth cycle shortened compared with normally sown and cultivated seedlings, and are developed in root systems, exuberant in growth vigor and capable of repeated regeneration and utilization.
Owner:秦少闻 +3

New technology of inducing clustered shoots from parispolyphylla rhizome for rapid vegetative propagation

The invention provides a new technology of inducing clustered shoots from parispolyphylla rhizome for rapid vegetative propagation. The new technology comprises: selecting a parispolyphylla rhizome to keep in reserve, drying in air for 3-7 days, making incisions on the back of the rhizome by using a cutter, immersing the incisions in an ABT rooting powder solution with a concentration of 10-30 ppm fro 2-10 h, disinfecting the incisions by plant ash, coating the parispolyphylla rhizome with a mixture of plant ash and clean river sands, transplanting and cultivating the parispolyphylla rhizome according to strains, coating fine soil with thickness of 5 mm on the parispolyphylla rhizome. After planting the rhizome, cluster buds germinate from stock plants and grow for about one year, then each parispolyphylla stock plant generates about more than twenty plantlets each of which possesses buds, small rhizome and a fine root, and the plantlets are taken as parispolyphylla seedlings for transplanting, and after 3-4 years, parispolyphylla finished products are obtained. The new technology has high propagation coefficient, and the produced parispolyphylla plantlets have high survival rate and fast growth speed. Compared with parispolyphylla seed propagation, the new technology helps to shorten more than one third of growth time of a finished product; and compared with a parispolyphylla rhizome fragment propagation method, the new technology helps to improve propagation coefficient by 4-6 times, and helps to solve the raw material gap facing medicinal materials and industrial production.
Owner:SICHUAN TONGDAOTANG PHARMA GROUP

Louis iris tissue culture fast seedling establishment and ecological application method

The invention relates to a tissue culture, rapid seedling formation and ecological application method for Louisiana iris, which comprises the following steps: 1) material selection: flowers, flower buds, floral axis, blades, roots and other vegetative organs of Louisiana iris are selected as explants; inoculation is carried out after routine disinfection; 2) bud induction and proliferation culture: the sterilized vegetative organs in 1) are inoculated on an induction medium; when callus has an area of 1 to 2 cm2, and forms a 0.5 to 0.8cm green convex block, the vegetative organs are transplanted in a differentiation medium; when the length of the vegetative organs reaches 5 to 8cm, the vegetative organs are transplanted in a rooting medium; 3) root induction: rootless seedlings with consistent growth are transplanted to a root induction medium; when the length of the rootless seedlings reaches 12 to 15cm, seedling adaptation is performed; 4) seedling adaptation: seedling adaptation is carried out for 6 to 8 days under natural light, then seedling emergence can be realized; culture medium at the root section of the seedlings is washed off; the seedlings are transplanted to a sterilized seedling container equipped with mixed substrate; after watering, shading and heat insulation, seedling emergence can be realized. The method has the advantages of fast seedling formation and good quality, and the seedlings can be directly applied to ecology.
Owner:吴月燕

Method for inducing embryonal-suspensor mass (ESM) regeneration plant from immature seed of pinus massoniana

The invention relates to a method for inducing an embryonal-suspensor mass (ESM) regeneration plant from an immature seed of pinus massoniana. By using the characteristic of continuous schizogenesis capability of the ESM in the immature seed of the pinus massoniana, solid culture and liquid culture are performed alternatively and ESM forms a completely-grown embryo with a normal structure after a maintaining stage, a multiplication stage, an embryo pre-mature stage and a maturation culture stage, so that a pinus massoniana plant is obtained by germinating and culturing the embryo. Due to the adoption of the method, a large number of regeneration plants of a pinus massoniana embryo of which the hereditary basis is accordant can be obtained and new technology with a short period and high occurring efficiency is provided for the storage of pinus massoniana rare generic resource and the mass production of excellent-gene nursery-grown plants.
Owner:NANJING FORESTRY UNIV

Quick propagation seedling-breeding method for reducing vitrifaction in multiple times of subculture of rosaceous plant

InactiveCN101965797AShorten the cycle of selection and breedingEase of mass productionHorticulture methodsPlant tissue culturePlant hormoneVitrification
The invention provides a quick propagation seedling-breeding method for reducing vitrifaction in multiple times of subculture of a rosaceous plant. The method comprises disinfection of explants, induction of buds, sub propagation and rooting induction. The method is characterized in that: in the sub propagation process, test tube seedlings are subjected to rejuvenation culture alternately, and then vigorous test tube seedlings with height of 1 to 3 centimeters are selected and transferred into a rooting culture medium for induced rooting so as to obtain complete test tube plants, wherein a starting culture medium without containing any exogenous plant hormone for rejuvenation is adopted in the rejuvenation culture. The method is quick, efficient, low in cost and convenient for popularization, has good genetic stability and low vitrifaction ratio in the culture process, keeps the propagation coefficient in a high level, and is suitable for tissue culture and quick propagation of the rosaceous plant.
Owner:JIANGSU ACAD OF AGRI SCI

Cutting and seedling method for phoebe shearer

The invention discloses a cutting and seedling method for phoebe shearer. The cutting and seedling method includes the steps: treating a cutting bed, namely paving cobblestones under a cutting bed and a layer of screen on the cutting bed and paving clean river sand for sterilization and disinfection of soil; collecting and producing cutting slips, namely clipping semi-lignification branches which are normal in growth with full shoots and without diseases and insect pests as cuttings from a phoebe shearer seed tree of 5-8 years from March to April to produce cutting slips; subjecting the produced cutting slips to early stage treatment and cutting; performing management after cutting. The cutting and seedling method is simple in operation, high in survival rate and capable of providing fine phoebe shearer seedlings, shortening breeding time of fine seeds and protecting excellent genetic traits of the seedlings. Therefore, propagation coefficient is greatly increased, and the survival rate reaches 80% at least.
Owner:SICHUAN AGRI UNIV

Bletilla germchit propagation method

The invention provides a bletilla germchit propagation method, belonging to the technical field of Chinese medical herb cultivation. The method consists of five steps of preparation of propagation field, preparation of protospecies, field planting propagation, seedbed management and germchit coming out of nursery, comprising the following steps: fertilizing 2-3kg of thoroughly decomposed farmyard manure, 50-55g of compound fertilizer and 15-17g of 80% soluble dipterex powder agent in the protected field to be evenly mixed with the soil; selecting the bletilla tuber having 3-5g of single tube with at least one hibernaculum as the protospecies; digging transversely shallow furrows with depth of 5-8cm on the border checks with the space between rows of 6-8cm*8-10cm, the seedbed soil humidity of 60-70%, and the room temperature of 15-20 DEG C; and covering a black lightproof net with the lightproof rate of 70-75% when the germchits coming out of the nursery. In the invention, the propagation period is one year earlier than the contrast and the average income of the germchits per mu can achieve * yuan. As the quality of the germchits is good, each mu can increase 100-240kg of production, thus increasing income.
Owner:云南省农业科学院高山经济植物研究所 +1

Cottage breeding method of sabina przewalskii

The invention discloses a cottage breeding method of sabina przewalskii, comprising the following steps: manufacture of a cottage seedling bed: manufacturing a seedling bed on the seedling culturing ground in a greenhouse in the spring of the current year; and covering fine sand and mycorrhiza soil as cottage matrix; strip picking: in the middle of May to June, selecting healthy, strong, pest and disease-and-damage-free sabina przewalskiiseedlings at the age of 3-5 with good growth and development for picking strips; and cutting processing: placing the cutting into clear water for soaking, drying rapidly, respectively placing into ABT1 rooting powder and potassium permanganate solution for respectively soaking for one time and then carrying out cottage; digging holes on the cottage seedling bed and obliquely cutting, and compacting the cutting hole; leading the cutting bed and the cutting to be fully contacted; spraying with carbendazim or Tuzet and copper abietate for once every half a month, thus preventing root rot of sabina przewalskii seedlings. The method of the invention greatly improves propagation coefficient, can shorten seeding culturing period, has high cottage seedling culturing survival rate, and can enable the cultured nursery stock to keep excellent characters of parent trees.
Owner:GANSU PROVINCE ACAD OF QILIAN WATER RESOURCE CONSERVATION FORESTS RES INST

Tissue culturing method for quercus virginiana

A tissue culturing method for quercus virginiana belongs to plant tissue culturing methods and is characterized by comprising the following steps: 1 selecting of explants; 2 disinfection of the explants; 3 primary culture; 4 multiplication culturing of adventitious buds; 5 culturing of strong seedlings; 6 culturing of strike roots; and 7 seedling acclimatization and transplanting. The tissue culturing method for the quercus virginiana can keep stress resistance and enjoyment of a female parent and can effectively increase propagation coefficient of the quercus virginiana. Compared with seminal propagation, tissue culturing reduces dependency on imported seeds, can effectively reduce nursery stock cost, and saves expenditures. Compared with cutting propagation, the tissue culturing is short in seedling culturing period, can obtain the nursery stock with strike roots within 4 months, and saves cost.
Owner:RES INST OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY +2

Chrysanthemum one-step tissue culture quick breeding method

The invention discloses a chrysanthemum one-step tissue culture quick breeding method. The method comprises the following steps of S1, obtaining aseptic tissue culture seedlings, collecting chrysanthemum explants, cutting the chrysanthemum explants into sections with buds and stems, sterilizing, cleaning, inoculating the sections to a culture medium to grow, transferring the sections to a new culture medium to grow after the new buds grow to be 2 to 3cm, and obtaining the aseptic robust maternal seedling with roots; and S2, one-step quick breeding, cutting the aseptic robust maternal seedlingobtained in S1 into sections with buds and stems, inoculating the sections to the culture medium to carry out passage and rooting culture, and enabling the sections with buds and stems to grow into complete plants. The method has the beneficial effects that the chrysanthemum tissue culture seedlings with roots do not need special seedling training, the robust growth is realized, the production cost is low, the genetic property of the obtained tissue culture seedling is uniform, the breeding coefficient is high, the breeding period is short, and the method is an effective path for the chrysanthemum tissue culture quick breeding; the culture medium of the tissue culture and the culture medium of the robust seedlings and roots belong to the same type of culture medium, so the operation difficulty is reduced, the culture efficiency is improved, and the application range is wide.
Owner:SICHUAN COLORLINK CO LTD

Tissue culture and intermediate propagation method for succulent Haworthia cooperivar. pilfera M. B. Bayer

The invention discloses a tissue culture and intermediate propagation method for succulent Haworthia cooperivar. pilfera M. B. Bayer. The method comprises the following steps: selecting Haworthia cooperivar. pilfera M. B. Bayer leaves in a good growing state as explants, after disinfecting the explants, inoculating the disinfected explants to clustered shoot inducting medium for inducing clustered shoots, then placing the clustered shoots into a rooting medium for taking roots, or after subculturing of the clustered shoots, placing the clustered shoots into the rooting medium for taking roots, and then carrying out seedling adaptation and plant transplanting on intact plantlets after root growing. According to the method, Haworthia cooperivar. pilfera M. B. Bayer seedlings can be obtained by tissue culturing to Haworthia cooperivar. pilfera M. B. Bayer leaves, the material used in the method is easy to obtain, the disinfecting is convenient, the survival rate is high, the production period is short, the propagation coefficient and the propagation speed of the Haworthia cooperivar. pilfera M. B. Bayer are improved, the strain advantages of the Haworthia cooperivar. pilfera M. B. Bayer can be well kept, a great amount of excellent Haworthia cooperivar. pilfera M. B. Bayer seedlings suitable for transplanting can be propagated quickly, the production demands are met, and the economic benefits of planters are improved.
Owner:SHAANXI NORMAL UNIV

Generative propagation breeding technology of dendrocalamus giganteus munro

The invention relates to a generative propagation breeding technology of dendrocalamus giganteus munro, comprising the following steps: seed collection and selection, seed storage, seed sowing and culture of seedling, preparation before planting bag seedling, planting, cultivation and protection, selective breeding of bamboo seedling, pest control, seed selection and survey of growth of the dendrocalamus giganteus munro. The sowing and the breeding of the invention has the characteristics of high reproduction coefficient, strong adaptability, strong stress resistance, ordered forest form, long life cycle, low afforestation expense, small labor intensity and the like.
Owner:GUIZHOU CHITIANHUA PAPERS
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