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Chrysanthemum one-step tissue culture quick breeding method

A technology for tissue culture and rapid propagation of chrysanthemums, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of complicated operation steps and increased difficulty, and achieve the effects of improving cultivation efficiency, reducing operation difficulty, and growing robustly

Active Publication Date: 2013-02-27
SICHUAN COLORLINK CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patent 201110029825.2 discloses the technology of tissue culture and rapid propagation of Stevia rebaudiana seedlings, but its operation steps are complicated, and each culture stage requires a medium with different components, which increases the difficulty of actual operation

Method used

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  • Chrysanthemum one-step tissue culture quick breeding method
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  • Chrysanthemum one-step tissue culture quick breeding method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Chrysanthemum one-step tissue culture rapid propagation method, it comprises the following steps:

[0018] S1. Obtain chrysanthemum aseptic tissue culture seedlings: collect chrysanthemum explants, cut into 2-3cm budding stem segments, sterilize with alcohol for 30 minutes, sterilize with mercury chloride for 5 minutes, rinse with sterile water 5 times, and inoculate on the culture medium grow like figure 1 As shown, the medium is MS+NAA0.2mg / L+IAA0.1mg / L, cultivated under the conditions of light 2000LX, 16 h, and temperature 24°C; 3cm, transfer to a new medium for growth, the medium is still MS+NAA0.2mg / L+IAA0.1mg / L, culture under the conditions of light 2000LX, 16 h, temperature 24°C, After two weeks of growth, obtain rooted aseptic strong female plantlets, such as figure 2 shown;

[0019] S2, one-step rapid propagation: cut the sterile and strong female parent seedlings obtained in step S1 into 20-30 sections of 2-3cm budding stem sections, inoculate them on the ...

Embodiment 2

[0021] Chrysanthemum one-step tissue culture rapid propagation method, it comprises the following steps:

[0022] S1. Obtain chrysanthemum aseptic tissue culture seedlings: collect chrysanthemum explants, cut into 2-3cm budding stem segments, sterilize with alcohol for 20 seconds, sterilize with mercury chloride for 6 minutes, rinse with sterile water 4 times, and inoculate on the culture medium grow like figure 1 As shown, the medium is MS+NAA0.2mg / L+IAA0.1mg / L, cultivated under the conditions of light 2000LX, 16 h, and temperature 24°C; 3cm, transfer to a new medium for growth, the medium is still MS+NAA0.2mg / L+IAA0.1mg / L, culture under the conditions of light 2000LX, 16 h, temperature 24°C, After two weeks of growth, obtain rooted aseptic strong female plantlets, such as figure 2 shown;

[0023] S2, one-step rapid propagation: cut the sterile and strong female parent seedlings obtained in step S1 into 20-30 sections of 2-3cm budding stem sections, inoculate them on the ...

Embodiment 3

[0025] Chrysanthemum one-step tissue culture rapid propagation method, it comprises the following steps:

[0026] S1. Obtain chrysanthemum aseptic tissue culture seedlings: collect chrysanthemum explants, cut them into 2-3 cm bud stem segments, sterilize with alcohol for 40 seconds, sterilize with mercury chloride for 4 minutes, rinse with sterile water 4 times, and inoculate on the culture medium grow like figure 1 As shown, the medium is MS+NAA0.2mg / L+IAA0.1mg / L, cultivated under the conditions of light 2000LX, 16 h, and temperature 24°C; 3cm, transfer to a new medium for growth, the medium is still MS+NAA0.2mg / L+IAA0.1mg / L, culture under the conditions of light 2000LX, 16 h, temperature 24°C, After two weeks of growth, obtain rooted aseptic strong female plantlets, such as figure 2 shown;

[0027] S2, one-step rapid propagation: cut the sterile and strong female parent seedlings obtained in step S1 into 20-30 sections of 2-3cm budding stem sections, inoculate them on th...

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Abstract

The invention discloses a chrysanthemum one-step tissue culture quick breeding method. The method comprises the following steps of S1, obtaining aseptic tissue culture seedlings, collecting chrysanthemum explants, cutting the chrysanthemum explants into sections with buds and stems, sterilizing, cleaning, inoculating the sections to a culture medium to grow, transferring the sections to a new culture medium to grow after the new buds grow to be 2 to 3cm, and obtaining the aseptic robust maternal seedling with roots; and S2, one-step quick breeding, cutting the aseptic robust maternal seedlingobtained in S1 into sections with buds and stems, inoculating the sections to the culture medium to carry out passage and rooting culture, and enabling the sections with buds and stems to grow into complete plants. The method has the beneficial effects that the chrysanthemum tissue culture seedlings with roots do not need special seedling training, the robust growth is realized, the production cost is low, the genetic property of the obtained tissue culture seedling is uniform, the breeding coefficient is high, the breeding period is short, and the method is an effective path for the chrysanthemum tissue culture quick breeding; the culture medium of the tissue culture and the culture medium of the robust seedlings and roots belong to the same type of culture medium, so the operation difficulty is reduced, the culture efficiency is improved, and the application range is wide.

Description

technical field [0001] The invention relates to the technical field of chrysanthemum cultivation methods, in particular to a one-step tissue culture and rapid propagation method for chrysanthemums. Background technique [0002] Chrysanthemum, a perennial herb of Compositae, is one of the top ten famous flowers in China and has a long history of cultivation and breeding. Chrysanthemum is also a commonly used traditional Chinese medicine in China. It has the effects of dispelling wind, clearing away heat, improving eyesight and detoxifying. Mainly treat headache, dizziness, conjunctival congestion, dysphoria in the heart and chest, treatment of sores, swelling and poisoning. Modern pharmacological studies have shown that chrysanthemum has various pharmacological activities such as treating coronary heart disease, lowering blood pressure, preventing hyperlipidemia, antibacterial, antitoxin, anti-inflammatory, and anti-aging. [0003] Chrysanthemum propagation methods generall...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 马建华冯爱云罗丽君王小辉晏强
Owner SICHUAN COLORLINK CO LTD
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