18results about How to "Improve cultivation efficiency" patented technology

The utility model relates to the technical field of microorganism culture, in particular to a microorganism constant-temperature incubator which comprises a base, an incubator main body and two sealing doors, the two sealing doors are movably connected to the two sides of the front end of the incubator main body through hinges, and a microorganism stable placement structure is arranged at the lower end of the inner side of the incubator main body. A microorganism stable heat supply and constant temperature keeping structure is arranged at the upper part in the incubator main body. According to the microorganism constant-temperature incubator, a microorganism culture solution is directly put into the microorganism culture dishes, then the sealing door at the front end of the incubator main body is opened, the bearing plate and the microorganism culture dishes above transversely slide into the incubator main body, and then the sealing door is closed; due to the transverse limitation of the limiting groove, the bearing plate can only drive the microorganism culture dish above to move to a designated position to be placed, and the subsequent microorganism culture process does not shake or have more other interference items, so that the microorganism culture process is not easily influenced by the microorganism constant-temperature incubator.

The invention belongs to the technical field of fruit cultivation, and particularly relates to an avocado rootstock asexual propagation method which comprises the following specific steps: material preparation, grafting and yellowing treatment, cup sleeving of a micro container and rooting promotion, rooting inspection, scion grafting, cutting and independent seedling hardening, and outplanting and transplanting. Compared with vegetative propagation, seedling propagation and tissue culture, the method has the advantages of high efficiency, multi-bud synchronization, high sapling consistency, root system and crown balance, full play of genetic potential, low transportation cost, high quarantine safety, good root system quality, high field planting survival rate and no rejuvenation period, and is suitable for large-scale, commercial, long-distance transportation and facility cultivation.

This utility model provides a chive cultivation device. The chive cultivation device includes: a cultivation box; a cultivation tray for planting chives, the cultivation tray being disposed on the cultivation box, the bottom of the cultivation tray having multiple ventilation holes; a draining plate, disposed inside the cultivation box, the draining plate having a filter cloth, the draining plate being located below the cultivation tray; a water holding tank, disposed inside the cultivation box, the water holding tank being located below the draining plate; and a storage box, the storage box being fixedly installed on the top of the cultivation box, the storage box having a partition fixedly installed inside, the partition dividing the storage box into a fertilizer storage chamber for storing organic liquid fertilizer and a water storage chamber. The chive cultivation device provided by this utility model can effectively separate excess water from the culture medium, realizing water recycling, and simultaneously cleaning and recycling the culture medium, avoiding waste of the culture medium.

The application discloses a kind of customized training plan generation methods for operation and maintenance personnel based on performance prism, which comprises the following steps: collecting multi-source heterogeneous demand data of operation and maintenance personnel on multiple platforms, and combining Carnot model and importance performance analysis model for fusion processing, extracting key technology theme through cluster analysis, drawing core training intention and decomposing into key measurement indicators, collecting employee daily operation and maintenance behavior data and calculating ability membership degree vector, combining post demand information, calculating target ability information to be enhanced, generating training content using intelligent workflow engine according to target ability information and key measurement indicators, generating hybrid training plan combining online and offline according to employee historical schedule, issuing the plan to terminal and setting task reminders, which can ensure that training is executed on time, improve training coverage and completion rate, and realize the intelligentization, customization and high efficiency of operation and maintenance personnel training from demand analysis, capability assessment, content generation to plan execution.

The utility model relates to the technical field of cell biology, in particular to a cell culture bag which comprises a bag body and at least one connector assembly. The connector assembly comprises a liquid discharging pipe and at least two supporting rods supporting the inner wall of the bag body, one end of the liquid discharging pipe is communicated with the bag body, the supporting rods are connected to the end, located in the bag body, of the liquid discharging pipe, and the supporting rods are arranged in the axis direction of the liquid discharging pipe. A liquid discharging gap is formed between every two adjacent supporting rods; the packaging bag has the effect of improving liquid outlet blockage caused by bag body attachment.

The utility model discloses a three flow channel runway formula open pool for microalgae culture, including open pool body, the open pool body be equipped with two side -by -side arrangement's partition wall, the partition wall will the space in open pool body divide into three side -by -side's flow channel, respectively two side flow channel and an intermediate flow channel, the both ends of adjacent two flow channels all communicate with each other, be equipped with the stirring paddle for driving microalgae culture solution flow in one end of intermediate flow channel. The utility model discloses the traditional two flow channel of runway formula open pool is improved to three flow channel, can effectively promote the flow rate of microalgae culture solution, reduce the mixed dead area, prevent microalgae deposition, reduce the collapse rate, improve microalgae culture efficiency. Especially suitable for the runway formula open pool of larger scale, reduce the energy consumption of same flow rate.

The invention relates to the technical field of microalgae culture devices, in particular to an efficient microalgae culture device and a using method thereof.The efficient microalgae culture device comprises a first-stage raceway pond, a second-stage raceway pond and a third-stage raceway pond which are sequentially stacked from bottom to top, and a partition plate is arranged at the bottom of a pond body of each stage of raceway pond; the horizontal areas of the three layers of raceway ponds are sequentially reduced from bottom to top, and a first-stage stirring system, a second-stage stirring system and a third-stage stirring device are arranged at one time corresponding to each stage of raceway pond; wherein each of the first-stage stirring system and the second-stage stirring system comprises a stirring device and a drainage device, the drainage device comprises a backflow channel and a drainage pipeline, and a lighting system is arranged at the bottom of each stage of raceway pond; the raceway ponds are arranged on the basis of a multi-layer stacking mode, and the effective culture liquid volume in unit occupied area is effectively increased; meanwhile, through the drainage mechanism, culture solution flow inoculation in the multi-stage raceway pond is achieved, the gas-liquid mass transfer efficiency is improved, the inoculation amount and the initial algae cell concentration are greatly improved, the culture period is shortened, and the culture efficiency is improved.

The utility model discloses a DC-CIK cell culture kit which comprises at least one DC bottle used for culturing DC cells; the at least one CIK bottle is detachably connected to the DC bottle and is used for culturing CIK cells; the at least one DC-CIK culture bottle is detachably connected to the DC bottle or the CIK bottle and is used for culturing DC-CIK cells; the culture bag is detachably connected to the DC-CIK culture bottle through a hose, and an opening and closing mechanism is arranged on the hose. According to the utility model, the whole-process culture of the DC-CIK cells can be completed on the premise that the cells are not transferred, the risk in the cell culture process is reduced, meanwhile, the technical requirements on producers can be reduced, and the cell culture efficiency is improved.

The utility model relates to the technical field of fermented bean curd production equipment, concretely is fermented bean curd mould culture and uses multilayer three -dimensional culture frame device, including the incubator and install in the incubator cultivation frame, the cultivation frame includes a plurality of parallelly arranged placing plate, the placing plate outer ring wall with the incubator inner wall close -fit, every placing plate top all are equipped with a temperature and humidity sensor, and two lifting rods are symmetrically equipped between every two placing plates. The utility model discloses through setting up lifting rod and guide rod, can adjust the distance between placing plate according to need, convenient operation and management, can also adapt to different specifications culture container simultaneously, still through setting up multilayer placing plate, has improved space utilization rate greatly, is suitable for large -scale mould culture, has reduced production cost.

The invention belongs to the technical field of water treatment, and particularly relates to an integrated microbial culture water treatment system which comprises a filter box, a fermentation box, a treatment box and a filter tank, a fine filter screen and a coarse filter screen are mounted on the upper side and the lower side of an inner cavity of the filter box respectively, and the lower side of the filter box is connected with a sewage inlet pipe; the sewage inlet pipe is located on the lower side of the coarse filter screen, a first communicating pipe is arranged on the upper side of the filter box, located on the upper side of the fine filter screen and connected with a treatment box, and a second communicating pipe is connected to the bottom of the fermentation box and communicated with the treatment box. A fixed seat is connected to the lower end of the left side of the treatment box through bolts, an aeration generator and an aeration pipe are installed on the fixed seat, the aeration generator is located on the outer side of the treatment box, pollutants in water are utilized or converted by microorganisms, the concentration of the pollutants is reduced, then the pollutants are filtered through a filtering tank, free microorganisms are removed through a filtering membrane, and the pollutants are purified. Therefore, water treatment is completed.

The invention relates to the technical field of haematococcus culture, in particular to a chelating agent-based haematococcus culture solution heavy metal passivation device which comprises a buffer tank for collecting a culture solution, a power box arranged below the bottom of the buffer tank and a stirring barrel arranged in the buffer tank, a plurality of feeding barrels are arranged at the top of the buffer tank and are configured into an acid and alkali liquor feeding barrel, a chelating agent feeding barrel and a flocculating agent feeding barrel; the device further comprises a central control unit, and the central control unit controls the amount of the chelating agent released by the chelating agent feeding barrel according to detection information of the heavy metal concentration sensor. By arranging the integrated buffer tank and the step-by-step adding system, pretreatment and precise reaction of the culture solution are completed, and the effect of preventing heavy metal poison from the source is achieved; by arranging a linkage mechanism of the detection tank and the return pipeline, the technical effect of terminal verification and guarantee of the effluent quality is achieved.

This utility model discloses a cultivation device for tissue culture seedlings, belonging to the technical field of cultivation devices. The device further includes an upper box body snapped onto the upper side of a base box; a second water pump is fixedly connected to the right side of the base box; a first water pump is fixedly connected to the right side of the upper box body; a water pipe is fixedly connected to the right side of the first water pump; a water tank is disposed on the outside of the base box; the second water pump and the water pipe are connected to the water tank; an atomizing nozzle is fixedly connected to the left side of the first water pump; a pair of sealing plugs are embedded on the front and rear sides of the upper box body respectively; by setting gauze mesh and ventilation holes, the air permeability of the tissue culture seedling roots is improved. Simultaneously, the gauze mesh prevents the soil and moisture in the soil from diffusing outwards, allowing a large amount of water to flow out from the mesh plate. The gauze mesh only absorbs a portion of the water and becomes moist, which is beneficial for the rapid growth of the tissue culture seedling roots. At the same time, the sealing plugs allow the tissue culture seedling leaves to come into contact with the air, facilitating control of the air exchange time between the inside of the upper box and the outside environment, thus improving cultivation efficiency.

The invention provides a recombinant cell construction and function verification system, which belongs to the technical field of biomedical engineering, and mainly comprises a reactor array, a material supplementing module, a sampling module, a detection module and a control unit, according to the system, by establishing an automatic feedback mechanism of online sampling, real-time detection and closed-loop feeding, the post-translational modification state of a recombinant cell can be obtained, accurate dynamic regulation and control of key quality attributes of target protein are achieved, and the culture efficiency and the sampling amount of a sampling module are remarkably improved through a reactor array, so that the quality of the target protein is improved. The yield and the quality stability of the complex recombinant protein are ensured.

The utility model discloses an NK (Natural Killer) cell culture device, which relates to the technical field of biological cell culture and comprises a base, a support structure, a power transmission system, a bearing and adjusting mechanism and a motion conversion component, the base is used for bearing and stabilizing other parts, a first support seat is fixedly arranged on the base and is used for supporting a motor and a transmission part thereof, and a second support seat is fixedly arranged on the base and is used for supporting the motor. The motor drives the first gear to rotate through the output shaft, the first gear is meshed with the first rotating shaft, one end of the first rotating shaft is connected with the second gear, the second gear is connected with the rotating plate through a key groove, the rotating plate is connected with the sliding block through the second rotating shaft, the sliding block slides along the rack, and the rack is fixed to the fixed guide rail. The NK cell culture device provided by the utility model effectively solves the problem of cell sedimentation caused by the gravity action, promotes the uniform distribution of cells, improves the culture efficiency, reduces the operation frequency of experimenters, reduces the pollution risk, and meets diversified experiment requirements.

The utility model discloses a corophium, algae-eating worm and algae microorganism culture device, which belongs to the technical field of microorganism culture and is characterized in that the bottom of an upper culture pond is communicated with a lower water storage pond, and a water pump is communicated between the upper culture pond and the lower water storage pond; the upper culture pond and the lower water storage pond can be arranged in an indoor and outdoor artificial pond body and can also be arranged in a surface water body; the external auxiliary mechanism comprises a connecting floating assembly, the connecting floating assembly is connected to the exterior of the top of the culture pond in a sleeving mode, the top of the culture pond is connected with a protective cover assembly, the protective cover assembly comprises a sunshine cover, and the sunshine cover is installed at the top of the upper culture pond; a sunshine cover in the protective cover assembly can effectively prevent outside sundries from entering the culture pond, interference on microorganism culture is avoided, the heat preservation effect can be achieved, natural light can be fully utilized, the photosynthesis requirement of algae microorganisms is met, and growth and reproduction of the algae microorganisms are promoted.

The utility model discloses a mixing device for cell culture, and mainly relates to the field of cell culture. Comprising a base and a culture cylinder, the top of the culture cylinder is movably connected with a top cover, the bottom of the culture cylinder is rotatably connected to the base, a driving cavity is formed in the base, a rotating seat is rotatably connected in the driving cavity, a fixing column is arranged at the bottom of the culture cylinder, and a power assembly for driving the rotating seat to rotate is arranged in the driving cavity; ejector rods are symmetrically and slidably connected to the base in the vertical direction, the tops of the ejector rods make contact with the bottom of the culture cylinder, and driving blocks for driving the ejector rods to slide in the vertical direction are symmetrically arranged in the driving cavity. The cell culture device has the beneficial effects that the technical problem that cells are easily damaged by mechanical stirring during cell culture so as to improve the culture failure rate and difficulty can be solved, the cell mixing efficiency is improved by driving the rotation and vertical movement of the culture cylinder, the possible damage to the cells is reduced, the integrity of the cells is ensured, and the cell culture efficiency is improved. And the success rate of cell culture is improved.

The utility model discloses a kind of culture devices for food detection, involve food detection field, including box, the inside of the box is provided with multiple placement grooves in annular array distribution, for placing culture medium;Cover plate, the cover plate is set in the top of box;Culture component, the culture component is set on cover plate;Culture medium, the culture medium is connected with the placement groove in the box by magnetic attraction, for the culture of the microorganism of food detection.The utility model is provided with multiple placement grooves in annular array distribution in the box, multiple culture medium is placed in different placement groove by magnetic attraction, so that the device can simultaneously cultivate different proportion of food, facilitate food detection, improve the culture efficiency of the device, box and sealing cover are provided on culture medium, so that the device is taken out culture medium for detection after opening box, the environment in culture medium does not contact with external environment, does not affect the result of detection.

The application belongs to the technical field of edible fungus physiological characteristic detection, and discloses a determination method and application of reed coprinus micaceus mycelium aging, which quantitatively evaluates the aging degree of mycelium by detecting the changes of carboxymethyl cellulase (CMC) enzyme activity and TTC (triphenyltetrazolium chloride) reductase activity in the reed coprinus micaceus mycelium. The application quantitatively determines the aging degree of the reed coprinus micaceus mycelium by establishing double-enzyme activity indexes. The CMC enzyme activity directly reflects the nutritional acquisition ability of the reed coprinus micaceus mycelium, and the TTC enzyme activity indicates the overall vitality of cells, so that the combination of the two can comprehensively capture the aging characteristics and avoid the limitations of a single index.