58results about How to "Improve cultivation efficiency" patented technology

The invention relates to the technical field of microalgae biological intelligent control, in particular to a microalgae growth prediction and optimization method and system based on machine learning. Based on the characteristic variable random forest model of the microalgae growth parameters, screening out a characteristic variable data set after dimensionality reduction screening which significantly affects the microalgae growth, and performing regression training of potential principal component variables on the characteristic variable data set through a machine learning algorithm to obtain a trained microalgae growth prediction model; acquiring a plurality of actual prediction results under dynamic change of culture conditions by using the trained microalgae growth prediction model, and searching a given parameter regulation space in a genetic algorithm based on the actual prediction results of microalgae growth culture to obtain an optimal culture condition parameter set; and issuing the optimal culture condition parameter set to an actual culture process of microalgae growth. According to the method, microalgae growth dynamics can be accurately described in a multi-dimensional parameter space, and real-time prediction and optimization of the microalgae growth trend and metabolic yield are realized.

The invention discloses application of a Wnt2 gene in buffalo ovarian granular cells, and belongs to the technical field of cell engineering and gene engineering. Wnt2 is taken as a research object, the Wnt2 overexpression vector or Wnt2 siRNA is transfected to the ovarian granulosa cells respectively by constructing the Wnt2 overexpression vector and synthesizing small interfering RNA (Wnt2 siRNA), then changes of signal channel gene mRNA and protein level related to ovarian granulosa cell apoptosis, proliferation and E2 and P4 secretion are detected, and finally phenotypic changes of the ovarian granulosa cells are detected. Results show that Wnt2 can inhibit apoptosis of ovarian granular cells and promote proliferation and E2 and P4 secretion. By exploring the application of the Wnt2 in the ovarian granular cells, the application has good application in researching an ovarian follicle atresia mechanism, initial estrus initiation and the like.

The invention discloses a cell preparation treatment method, system and medium, and the method comprises the following steps: carrying out cell amplification treatment on a to-be-cultured cell sap in a cell culture container to obtain a cultured cell sap; washing and concentrating the cultured cell sap in the cell culture container to obtain concentrated cell sap; and sub-packaging the concentrated cell sap in the cell culture vessel into a plurality of preparation bags. According to the invention, cell culture, cell washing and concentrating treatment and preparation sub-packaging are realized on the cell culture container, so that scattered arrangement of equipment is avoided, and the occupied space is reduced; the steps are sequentially completed through the cell culture container, the whole process from cell culture to cell preparation can be ensured to be performed in a sealed sterile environment, cell sap does not need to be transferred, and the cell culture quality is improved; the steps are sequentially and integrally completed through the cell culture container, manual intervention is reduced, the whole process from cell culture to cell preparation is intelligently carried out, and the cell culture efficiency is improved.

The invention relates to the field of sewage treatment, in particular to a fluidized bed reactor for sewage treatment, which comprises a vertical tank body, the bottom of the tank body is provided with a water feeder, the water feeder is connected with a water inlet pipe, the tank body is connected with a reflux pipeline, the lower end of the reflux pipeline is connected with the water feeder, and the higher end of the reflux pipeline is connected with a water absorption piece. The water absorption piece is located in the tank body and divides the interior of the tank body into a clear water area located above the water absorption piece and a fluidization area located below the water absorption piece, a circulating pump is arranged on the backflow pipeline, the fluidization area is provided with a plurality of water outlet pieces which are vertically arranged, a plurality of liquid outlet holes are formed in the water outlet pieces, and the water outlet pieces are all communicated with the backflow pipeline; and liquid sucked by the water suction piece enters the water inlet device and each water outlet piece through the backflow pipeline. According to the scheme, the problem that the fluidization effect of the middle of the fluidization area is poor is solved, and the fluidization effect of the middle of the fluidization area is improved.

The invention discloses application of an SFRP1 gene in buffalo ovarian granular cells, and belongs to the technical field of cell engineering and gene engineering. According to the invention, SFRP1 is taken as a research object, an SFRP1 overexpression vector or siRNA-SFRP1 is respectively transfected to ovarian granular cells by constructing the SFRP1 overexpression vector and synthesizing small interfering RNA (siRNA-SFRP1), then changes of signal channel gene mRNA and protein level related to ovarian granular cell apoptosis, proliferation and E2 and P4 secretion are detected, and finally phenotypic changes of the ovarian granular cells are detected. Results show that the SFRP1 can inhibit apoptosis of ovarian granular cells and promote proliferation and E2 and P4 secretion. The application of the SFRP1 in the ovarian granular cells is explored, and the SFRP1 has good application in research of an ovarian follicle atresia mechanism, initial estrus initiation and the like.

The invention belongs to the technical field of plant cultivation, and particularly relates to a wheat shallow-buried drip-irrigation uniform-sowing densification high-yield cultivation method and system, two main treatments of traditional sowing and uniform sowing are set, four different cultivation densities are set at the same time, and changes of wheat growth and yield under different treatments are observed; a drip irrigation tape laying mode of one pipe and two rows is adopted; two chemical control agents of chlormequat chloride and paclobutrazol * mepiquat chloride are sprayed in the three-leaf stage and the elongation stage of wheat, and the dosage of the chemical control agents is set to be three gradients; determining a chemical regulation and control scheme of the spring wheat under the close planting cultivation condition; under the condition of close planting, the control effect of close planting chemical control on wheat growth and yield is studied by adopting a split area test design, and the control effect of water and nitrogen control on wheat growth and yield is studied. According to the method, the planting density and the planting specification of a shallow-buried drip irrigation uniform sowing mode are defined, and a high-yield, high-quality and high-water-efficiency irrigation and fertilization scheme for wheat is determined.

The utility model discloses a multi-layer wheat water-saving cultivation device which comprises a cultivation cabinet and cultivation boxes, the cultivation cabinet comprises a cabinet body, and a plurality of supporting plates are arranged in the cabinet body; the cabinet body forms a plurality of cultivation areas through a plurality of supporting plates; a sliding rail is arranged in the cultivation area; a main water pipe is arranged outside the cabinet body, a plurality of branch water pipes are connected to the main water pipe, and the branch water pipes are connected into the cultivation areas respectively and located at the upper ends of the corresponding cultivation areas; the cultivation box comprises a box body, and sliding grooves embedded with the sliding rails are formed in the outer side of the box body of the cultivation box; the multiple cultivation boxes are installed on the supporting plates in the corresponding cultivation areas through the sliding grooves. The cultivation cabinet has the advantages that the interior of the cabinet body is divided into the cultivation areas through the supporting plates, the cultivation boxes are placed in the cultivation areas respectively, the water distribution pipes and the spraying pipes are arranged in the cultivation areas respectively, and the cultivation efficiency can be higher through the multi-layer structure.

The invention relates to the technical field of cultivation devices, in particular to a fruit and vegetable cultivation device which comprises a box body, mounting vertical plates are symmetrically arranged on the box body, a rotary cultivation mechanism for cultivating fruits and vegetables in batches is arranged on the mounting vertical plates, and a plant protection mechanism for preventing a nutrient solution from dripping onto the surfaces of the fruits and vegetables to cause damage is arranged in the rotary cultivation mechanism. A liquid supply mechanism for providing a nutrient solution for the roots of the fruits and vegetables is arranged in the box body, a light source supply mechanism is arranged above the box body, the rotary cultivation mechanism comprises a rotary support movably mounted on a mounting vertical plate, and a driving motor is fixedly mounted on the side, away from the rotary support, of the mounting vertical plate. By arranging the plant protection mechanism, a nutrient solution remaining on the upper open box body in the rotary cultivation process can be prevented from dripping onto the lower open box body through mutual cooperation between a solution receiving cylinder and a bearing assembly, and the phenomenon that the nutrient solution drips onto a fruit and vegetable body to generate adverse effects on the fruit and vegetable cultivation process can be effectively avoided.

The utility model discloses a mushroom bed earthing and fertilizing device for industrial cultivation of Pleurotus eryngii, which comprises a cultivation mushroom bed, a fertilizing box is arranged in the cultivation mushroom bed, symmetrical guide rail grooves are arranged on the upper surface of the cultivation mushroom bed, symmetrical driving motors are fixedly connected to the outer surface of the fertilizing box, and the driving motors are connected with the fertilizing box. The output ends of the two driving motors are fixedly connected with moving wheels, the two moving wheels are located in the two guide rail grooves respectively, the bottom surface of the fertilization box fixedly communicates with fertilization pipes arranged at equal intervals, the outer surface of each fertilization pipe is provided with a control valve, and the upper surface of the fertilization box is fixedly connected with a water tank. According to the device, the driving motor is matched with the moving wheels, so that the fertilization box can be driven to move stably, the control valve can be used for controlling the outflow of a fertilizer, the plurality of fertilization pipes can be used for uniformly fertilizing a mushroom cultivation bed, and the pump body is matched with the water spraying disc, so that the mushroom cultivation bed can be uniformly sprayed with water, and the effect of timely watering is achieved.

A bin entering and exiting structure belongs to the field of blood culture instruments and comprises a bin body, a driving mechanism, a sliding assembly and a bearing seat, the bin body comprises a bottom plate, the bearing seat is slidably mounted on the bin body through the sliding assembly, the driving mechanism is mounted on the bottom plate, the driving mechanism is in transmission connection with the bearing seat, and the driving mechanism is located below the bearing seat. The driving direction of the driving mechanism is the same as the guiding direction of the sliding assembly. According to the utility model, the driving mechanism is guided by the sliding mechanism to drive the bearing seat to automatically enter and exit from the bin, so that errors caused by manual operation are avoided, the blood culture efficiency is improved, and the bearing seat filled with a blood culture bottle can be pulled out by pulling the transmission belt under the condition of power failure due to belt wheel transmission.

The invention provides a mesenchymal stem cell preparation as well as a preparation method and application thereof, and relates to the technical field of biology, the mesenchymal stem cell preparation contains 5 * 10 < 6 >-1.2 * 10 < 7 > cells / mL of mesenchymal stem cells, 0.1-0.6% w / v of human serum albumin and a solvent; the mesenchymal stem cells are obtained by culturing a serum-free culture medium, and the mesenchymal stem cells are obtained by resuscitating cryopreserved mesenchymal stem cells, inoculating the resuscitated mesenchymal stem cells into a culture container, culturing until the mesenchymal stem cells are completely adhered to the wall, and digesting. The mesenchymal stem cell preparation improves the treatment effect of mesenchymal stem cells.

The invention discloses a cell culture container which comprises a container body, a connecting plate is arranged in the container body, and a plurality of through holes for cell sap to pass through are distributed in the connecting plate. The invention further discloses a shaking table which comprises a movable platform, a platform driver and the cell culture container, the container body is arranged on the movable platform, and the platform driver is used for driving the movable platform. The cell culture container and the shaking table have the advantages of being suitable for large-scale culture, capable of timely and uniformly stirring cell sap and the like.

The invention relates to the technical field of fungus material culture, and particularly discloses a fungus material culture method of armillaria mellea for artificial cultivation of gastrodia elata, which comprises the steps of fungus material selection, culture medium preparation, inoculation, culture shelf arrangement, environment arrangement, harvesting and gastrodia elata planting. According to the method, hard and corrosion-resistant broad-leaved tree species are selected as the fungus material, a composite culture medium formula is adopted, namely, the advantages of a liquid culture medium and a solid culture medium are combined, comprehensive and balanced nutrition support is provided for growth of armillaria mellea, the culture efficiency and quality of the fungus material are improved, meanwhile, the proportion of the formula of the culture medium is designed, and the culture cost is reduced. The water content and the pH value of the culture medium are controlled, favorable growth environment conditions are created for growth of armillaria mellea, meanwhile, a syringe or an inoculating loop for high-pressure steam sterilization effectively prevents contamination of infectious microbes, and the success rate of inoculation and the purity of hyphae are improved.

The utility model relates to the technical field of microorganism culture, in particular to a microorganism constant-temperature incubator which comprises a base, an incubator main body and two sealing doors, the two sealing doors are movably connected to the two sides of the front end of the incubator main body through hinges, and a microorganism stable placement structure is arranged at the lower end of the inner side of the incubator main body. A microorganism stable heat supply and constant temperature keeping structure is arranged at the upper part in the incubator main body. According to the microorganism constant-temperature incubator, a microorganism culture solution is directly put into the microorganism culture dishes, then the sealing door at the front end of the incubator main body is opened, the bearing plate and the microorganism culture dishes above transversely slide into the incubator main body, and then the sealing door is closed; due to the transverse limitation of the limiting groove, the bearing plate can only drive the microorganism culture dish above to move to a designated position to be placed, and the subsequent microorganism culture process does not shake or have more other interference items, so that the microorganism culture process is not easily influenced by the microorganism constant-temperature incubator.

The utility model relates to an agricultural product matched production device, in particular to a liquid strain fermentation tank which comprises a tank body, a stirring mechanism used for stirring liquid is arranged in the tank body in the vertical direction, a flushing mechanism is arranged at the top in the tank body, and the stirring mechanism comprises a motor, a rotating body and a stirring rod. The rotating body and the stirring rod are both of hollow structures, a plurality of blowholes are formed in the side face of the stirring rod in a penetrating mode, and a rotating connector is fixedly arranged at the bottom of the rotating body and fixedly connected with a breather pipe; the flushing mechanism comprises an annular pipe, a nozzle and a liquid conveying pipe; according to the utility model, the inside of the tank main body can be cleaned and sterilized quickly, and meanwhile, sufficient oxygen can be provided for the growth of strains in the strain culture process.

The invention belongs to the technical field of fruit cultivation, and particularly relates to an avocado rootstock asexual propagation method which comprises the following specific steps: material preparation, grafting and yellowing treatment, cup sleeving of a micro container and rooting promotion, rooting inspection, scion grafting, cutting and independent seedling hardening, and outplanting and transplanting. Compared with vegetative propagation, seedling propagation and tissue culture, the method has the advantages of high efficiency, multi-bud synchronization, high sapling consistency, root system and crown balance, full play of genetic potential, low transportation cost, high quarantine safety, good root system quality, high field planting survival rate and no rejuvenation period, and is suitable for large-scale, commercial, long-distance transportation and facility cultivation.

The equipment comprises a synthesis box, a culture medium is arranged in the middle of the inner side of the synthesis box, a stirring mechanism is arranged above the culture medium, the stirring mechanism is located on the inner wall of the synthesis box, the output end of the stirring mechanism is located on the inner side of the culture medium, a nutrition collection box is arranged on one side of the stirring mechanism, and the nutrition collection box is arranged on the other side of the synthesis box. One side of the bottom surface of the nutrition collection box is communicated with a measurement assembly, and the bottom of the measurement assembly is connected with an extraction conveying part; according to the device, various different nutrient solutions can be independently absorbed, a fixed amount of single nutrient solution is conveyed into the culture medium through the measuring assembly and the extracting and conveying part, absorption and growth of a carrier are provided, and after the nutrient solutions are supplemented, stirring and mixing of the nutrient solutions in the culture medium can be automatically completed by replacing different nutrient solutions, so that the nutrient solutions are completely supplemented. And the growth of the carrier is completely mechanically and automatically operated, so that the manual intervention is reduced, and the operation precision and the culture efficiency are higher.

This utility model provides a chive cultivation device. The chive cultivation device includes: a cultivation box; a cultivation tray for planting chives, the cultivation tray being disposed on the cultivation box, the bottom of the cultivation tray having multiple ventilation holes; a draining plate, disposed inside the cultivation box, the draining plate having a filter cloth, the draining plate being located below the cultivation tray; a water holding tank, disposed inside the cultivation box, the water holding tank being located below the draining plate; and a storage box, the storage box being fixedly installed on the top of the cultivation box, the storage box having a partition fixedly installed inside, the partition dividing the storage box into a fertilizer storage chamber for storing organic liquid fertilizer and a water storage chamber. The chive cultivation device provided by this utility model can effectively separate excess water from the culture medium, realizing water recycling, and simultaneously cleaning and recycling the culture medium, avoiding waste of the culture medium.

The application discloses a kind of customized training plan generation methods for operation and maintenance personnel based on performance prism, which comprises the following steps: collecting multi-source heterogeneous demand data of operation and maintenance personnel on multiple platforms, and combining Carnot model and importance performance analysis model for fusion processing, extracting key technology theme through cluster analysis, drawing core training intention and decomposing into key measurement indicators, collecting employee daily operation and maintenance behavior data and calculating ability membership degree vector, combining post demand information, calculating target ability information to be enhanced, generating training content using intelligent workflow engine according to target ability information and key measurement indicators, generating hybrid training plan combining online and offline according to employee historical schedule, issuing the plan to terminal and setting task reminders, which can ensure that training is executed on time, improve training coverage and completion rate, and realize the intelligentization, customization and high efficiency of operation and maintenance personnel training from demand analysis, capability assessment, content generation to plan execution.

The invention discloses a clinical laboratory anti-pollution bacterial culture device which comprises a first culture box and a second culture box, one side of the first culture box is hinged to one side of the second culture box, and a first handle is fixedly arranged on one side of the second culture box; a plurality of connecting plates are hinged to one side of the inner wall of the first culture box at equal intervals, and one side of each connecting plate is fixedly connected with one end of a transverse plate. According to the anti-pollution type bacterial culture device for the clinical laboratory, the oxygen solubility and distribution of a liquid culture medium can be enhanced, and the anti-pollution type bacterial culture device is especially important for aerobic bacteria; the bacteria can obtain sufficient oxygen, rapid and uniform growth is promoted, the culture efficiency is improved, nutrient substances in the culture medium can be uniformly distributed, the situation that the concentration of local nutrient substances is too high or too low is avoided, all the bacteria can uniformly obtain required nutrients, and the consistency of bacterial growth and the accuracy of experimental results are improved.

The utility model relates to the technical field of cell biology, in particular to a cell culture bag which comprises a bag body and at least one connector assembly. The connector assembly comprises a liquid discharging pipe and at least two supporting rods supporting the inner wall of the bag body, one end of the liquid discharging pipe is communicated with the bag body, the supporting rods are connected to the end, located in the bag body, of the liquid discharging pipe, and the supporting rods are arranged in the axis direction of the liquid discharging pipe. A liquid discharging gap is formed between every two adjacent supporting rods; the packaging bag has the effect of improving liquid outlet blockage caused by bag body attachment.

The utility model discloses a three flow channel runway formula open pool for microalgae culture, including open pool body, the open pool body be equipped with two side -by -side arrangement's partition wall, the partition wall will the space in open pool body divide into three side -by -side's flow channel, respectively two side flow channel and an intermediate flow channel, the both ends of adjacent two flow channels all communicate with each other, be equipped with the stirring paddle for driving microalgae culture solution flow in one end of intermediate flow channel. The utility model discloses the traditional two flow channel of runway formula open pool is improved to three flow channel, can effectively promote the flow rate of microalgae culture solution, reduce the mixed dead area, prevent microalgae deposition, reduce the collapse rate, improve microalgae culture efficiency. Especially suitable for the runway formula open pool of larger scale, reduce the energy consumption of same flow rate.

Relates to the field of bacterial culture, and discloses a temperature and humidity adjusting device in a bacterial culture process, which comprises an incubator and further comprises a collecting unit arranged on the inner bottom wall of the incubator and used for collecting a water source; the collecting unit comprises a water collecting tank which is arranged on the inner bottom wall of the incubator and is used for collecting a water source; the detection device is arranged in the water collecting tank and is used for detecting the water source amount in the water collecting tank; according to the utility model, the accumulation of a water source in the culture box can be reduced, the breeding of harmful bacteria can be reduced, the culture efficiency of bacteria can be improved, the water source in the water collection tank is purified and then is connected into the water supply pipe, and the water supply pipe is connected with the water collection tank. And the water collecting tank is made of stainless steel, so that the service life of the whole device can be prolonged.

The invention discloses a printing nozzle device, an extrusion type organoid 3D printer, a communication module and an organoid printing method. The printing nozzle device comprises a first propeller assembly, a first temperature control assembly and a first driving assembly. The first propeller assembly is provided with a first liquid guiding end used for guiding out cell ink, and the first temperature control assembly is connected with the first propeller assembly and used for adjusting and controlling the temperature of the cell ink guided out by the first propeller assembly to be within a first preset range. The first driving assembly is connected with the first propeller assembly and used for driving the first propeller assembly to move along a first preset path. And the first driving assembly is configured to enable the first propeller assembly to move towards the direction close to the surface of the receiving carrier until the cell ink guided out of the first liquid guide end is adhered to the surface of the receiving carrier, and then enable the first propeller assembly to move towards the direction away from the surface of the receiving carrier until the cell ink is separated from the first liquid guide end. The printing device is high in efficiency and precision, and can efficiently complete work such as printing of the similar organs.

The utility model relates to a microbial strain culture device which comprises a shell and a shell cover, a plurality of notches are annularly formed in the circumferential side wall of the shell, a strain culture frame capable of being pulled in the radial direction of the shell is clamped in each notch, and the top of each strain culture frame is open and lower than the top opening of the shell. A circulation gap is formed between every two adjacent strain culture frames, a plurality of vent holes communicated with inner cavities of the strain culture frames are correspondingly formed in the top of the shell cover, and a pipeline used for injecting water with the preset temperature into the inner cavity of the shell is fixedly arranged on one side of the shell. Different types of strains are respectively placed in the corresponding strain culture frames for simultaneous culture, so that the culture efficiency is greatly improved; due to the fact that the multiple strain culture frames are annularly arranged in the shell, the utilization rate of the strain culture frames on the storage space can be increased; due to the fact that the circulation gap is formed between every two adjacent strain culture frames, when water with the preset temperature is injected into the shell through the pipeline, heating of each strain culture frame can be uniform, and strains in each strain culture frame are in a suitable culture environment.

The invention discloses a method for separating, purifying and identifying tea cake disease pathogenic bacteria, which is characterized by comprising the following steps of: firstly collecting disease samples, then pretreating diseased leaves, then separating the pathogenic bacteria, then selecting hyphae for purifying and culturing, then respectively verifying the pathogenic bacteria and extracting DNA (deoxyribonucleic acid) of the pathogenic bacteria, and finally carrying out PCR (polymerase chain reaction) amplification and identification. According to the method, the ejection characteristic of mature basidiospore is fully utilized, the diseased tissue is directly fixed on the culture dish cover for isolated culture, the probability of contamination of endophyte and influence of infectious microbes is extremely low, the culture efficiency is improved, and tea cake disease pathogenic bacteria can be ensured; pCR amplification confirmation is carried out on the rDNA-ITS region of the pathogenic bacteria through universal primers ITS1 and ITS4 in a genome transcription spacer region, so that the selectivity of a target strain is enhanced, and the pathogenic bacteria can be rapidly identified.

The invention belongs to the technical field of molecular biology, and particularly relates to an SNP (Single Nucleotide Polymorphism) marker related to cold resistance of peaches, a primer group and application of the SNP marker. The SNP marker is named as KH001 and is located at the 13th, 291 and 544th basic group pairs of a No.4 chromosome, and the basic groups at the positions are C or G; the SNP marker is abbreviated to be Chr0413, 291, 544, C / G. The invention further discloses an application of the SNP marker. The invention also relates to a primer group for carrying out genetic typing identification on the SNP marker. The SNP marker and the primer group can be used as auxiliary selection tools, are used for early screening peach tree varieties with relatively good cold resistance, and have important values for improving the peach tree cultivation efficiency and adapting to agricultural production under a cold climate condition.

The invention relates to the technical field of microalgae culture devices, in particular to an efficient microalgae culture device and a using method thereof.The efficient microalgae culture device comprises a first-stage raceway pond, a second-stage raceway pond and a third-stage raceway pond which are sequentially stacked from bottom to top, and a partition plate is arranged at the bottom of a pond body of each stage of raceway pond; the horizontal areas of the three layers of raceway ponds are sequentially reduced from bottom to top, and a first-stage stirring system, a second-stage stirring system and a third-stage stirring device are arranged at one time corresponding to each stage of raceway pond; wherein each of the first-stage stirring system and the second-stage stirring system comprises a stirring device and a drainage device, the drainage device comprises a backflow channel and a drainage pipeline, and a lighting system is arranged at the bottom of each stage of raceway pond; the raceway ponds are arranged on the basis of a multi-layer stacking mode, and the effective culture liquid volume in unit occupied area is effectively increased; meanwhile, through the drainage mechanism, culture solution flow inoculation in the multi-stage raceway pond is achieved, the gas-liquid mass transfer efficiency is improved, the inoculation amount and the initial algae cell concentration are greatly improved, the culture period is shortened, and the culture efficiency is improved.

The utility model relates to the technical field of medical culture equipment, in particular to a positive pressure swing culture system, which comprises a closed box body, a mounting plate is fixedly mounted at one end of the bottom of the box body, a plurality of culture bottles communicated with the mounting plate are fixed in the mounting plate, a gas input end is arranged on the box body, and a gas outlet end is arranged on the gas input end. The other side of the gas input end is hermetically connected with a gas washing bottle arranged in the box body through a gas pipe, and the gas outlet end of the gas washing bottle is communicated with the mounting disc through a gas pipe. By controlling and adjusting the pressure and temperature of the culture environment and driving the culture bottle to move eccentrically, the natural growth environment of the embryonic cells in a living body is simulated, so that the growth and development of the embryonic cells are promoted, and the culture efficiency of the embryonic cells is improved.

The utility model discloses a DC-CIK cell culture kit which comprises at least one DC bottle used for culturing DC cells; the at least one CIK bottle is detachably connected to the DC bottle and is used for culturing CIK cells; the at least one DC-CIK culture bottle is detachably connected to the DC bottle or the CIK bottle and is used for culturing DC-CIK cells; the culture bag is detachably connected to the DC-CIK culture bottle through a hose, and an opening and closing mechanism is arranged on the hose. According to the utility model, the whole-process culture of the DC-CIK cells can be completed on the premise that the cells are not transferred, the risk in the cell culture process is reduced, meanwhile, the technical requirements on producers can be reduced, and the cell culture efficiency is improved.