241results about How to "Improve cultivation efficiency" patented technology

The invention relates to the technical field of microalgae biological intelligent control, in particular to a microalgae growth prediction and optimization method and system based on machine learning. Based on the characteristic variable random forest model of the microalgae growth parameters, screening out a characteristic variable data set after dimensionality reduction screening which significantly affects the microalgae growth, and performing regression training of potential principal component variables on the characteristic variable data set through a machine learning algorithm to obtain a trained microalgae growth prediction model; acquiring a plurality of actual prediction results under dynamic change of culture conditions by using the trained microalgae growth prediction model, and searching a given parameter regulation space in a genetic algorithm based on the actual prediction results of microalgae growth culture to obtain an optimal culture condition parameter set; and issuing the optimal culture condition parameter set to an actual culture process of microalgae growth. According to the method, microalgae growth dynamics can be accurately described in a multi-dimensional parameter space, and real-time prediction and optimization of the microalgae growth trend and metabolic yield are realized.

The invention relates to the field of microbial culture management, in particular to a microbial culture management system and method. The method comprises the following steps: calculating growth factors based on the number of microorganisms in a microorganism growth process and microorganism culture environment parameters which are collected in real time, and calculating a predicted value of the number of microorganisms at a future moment; detecting the growth state of the microorganisms based on the predicted value of the number of the microorganisms in the future; the initial adjustment amount of the microbial culture environment parameters is obtained through a multi-parameter nonlinear optimization algorithm, the final adjustment amount of the microbial culture environment parameters is obtained by introducing a synergistic effect mechanism, and the microbial culture environment parameters are adjusted. The problems that traditional microbial culture environment parameters depend on manual monitoring and experience adjustment, so that response is lagged, and adjustment is not accurate enough are solved; the future growth trend of microorganisms is difficult to accurately predict, so that the culture management reaction is delayed; the problems that the microorganism culture environment is imbalanced and optimal management cannot be realized due to the fact that microorganism culture environment parameters cannot be automatically optimized are solved.

The invention belongs to the technical field of microorganisms, and particularly relates to a microorganism detection culture device and method.The system comprises an intelligent environment control system, and a sensor monitors the temperature, humidity and gas concentration of a culture environment in real time to ensure that environment parameters needed by microorganism growth are kept in the optimal state; the automatic sample processing and operating system avoids pollution or errors caused by manual intervention through a mechanical arm or an automatic transmission device; the real-time image monitoring and analyzing system is used for monitoring the growth state of the microorganisms in the culture dish; the camera can continuously shoot images in the culture process; the rapid detection module rapidly obtains activity information of microorganisms, rapid detection is achieved, and time needed by traditional culture is shortened. According to the invention, accurate control, automatic operation and rapid detection are realized, and an efficient, safe and intelligent solution is provided for microbial culture and detection.

The invention discloses application of a Wnt2 gene in buffalo ovarian granular cells, and belongs to the technical field of cell engineering and gene engineering. Wnt2 is taken as a research object, the Wnt2 overexpression vector or Wnt2 siRNA is transfected to the ovarian granulosa cells respectively by constructing the Wnt2 overexpression vector and synthesizing small interfering RNA (Wnt2 siRNA), then changes of signal channel gene mRNA and protein level related to ovarian granulosa cell apoptosis, proliferation and E2 and P4 secretion are detected, and finally phenotypic changes of the ovarian granulosa cells are detected. Results show that Wnt2 can inhibit apoptosis of ovarian granular cells and promote proliferation and E2 and P4 secretion. By exploring the application of the Wnt2 in the ovarian granular cells, the application has good application in researching an ovarian follicle atresia mechanism, initial estrus initiation and the like.

The invention relates to the technical field of cell activity detection, in particular to an umbilical cord blood stem cell activity detection method and system.The method comprises the following steps that cells are separated from a fresh umbilical cord blood sample in a sterile environment, cell activity preliminary identification is conducted on the separated stem cells, and a cell activity evaluation result is generated; screening cells with activity meeting a preset standard, and performing function evaluation to obtain a screened cell set. According to the method, through accurate preliminary cell viability identification and function evaluation, the scheme optimizes the selection standard of umbilical cord blood stem cells, effectively screens out cells with high regeneration capacity and biological activity, enhances the success rate of cell therapy and reduces the risk caused by improper selection, and by analyzing the gene regulatory network of the screened cells, the cell therapy effect is improved. The functionality is accurately evaluated, it is ensured that only the most potential cells are used for subsequent culture and clinical application, the curative effect and the product quality are remarkably improved, and the culture efficiency and the success rate are further improved through an optimized culture scheme and a monitoring system.

The invention discloses a cell preparation treatment method, system and medium, and the method comprises the following steps: carrying out cell amplification treatment on a to-be-cultured cell sap in a cell culture container to obtain a cultured cell sap; washing and concentrating the cultured cell sap in the cell culture container to obtain concentrated cell sap; and sub-packaging the concentrated cell sap in the cell culture vessel into a plurality of preparation bags. According to the invention, cell culture, cell washing and concentrating treatment and preparation sub-packaging are realized on the cell culture container, so that scattered arrangement of equipment is avoided, and the occupied space is reduced; the steps are sequentially completed through the cell culture container, the whole process from cell culture to cell preparation can be ensured to be performed in a sealed sterile environment, cell sap does not need to be transferred, and the cell culture quality is improved; the steps are sequentially and integrally completed through the cell culture container, manual intervention is reduced, the whole process from cell culture to cell preparation is intelligently carried out, and the cell culture efficiency is improved.

The invention relates to the field of sewage treatment, in particular to a fluidized bed reactor for sewage treatment, which comprises a vertical tank body, the bottom of the tank body is provided with a water feeder, the water feeder is connected with a water inlet pipe, the tank body is connected with a reflux pipeline, the lower end of the reflux pipeline is connected with the water feeder, and the higher end of the reflux pipeline is connected with a water absorption piece. The water absorption piece is located in the tank body and divides the interior of the tank body into a clear water area located above the water absorption piece and a fluidization area located below the water absorption piece, a circulating pump is arranged on the backflow pipeline, the fluidization area is provided with a plurality of water outlet pieces which are vertically arranged, a plurality of liquid outlet holes are formed in the water outlet pieces, and the water outlet pieces are all communicated with the backflow pipeline; and liquid sucked by the water suction piece enters the water inlet device and each water outlet piece through the backflow pipeline. According to the scheme, the problem that the fluidization effect of the middle of the fluidization area is poor is solved, and the fluidization effect of the middle of the fluidization area is improved.

The utility model discloses a phycomycete symbiotic reactor for sewage treatment and a treatment system, and relates to the technical field of sewage treatment devices. Comprising a first treatment device, and the first treatment device comprises a first barrel body with a containing space; a light-emitting assembly is arranged in the first can body, the light-emitting assembly comprises a columnar main body part connected with the bottom of the first can body and forked parts extending in the radial direction of the main body part, and the forked parts are arranged in multiple layers in the length direction of the main body part; a power supply assembly is arranged at the outer bottom of the first barrel body and is electrically connected with the light-emitting assembly; by adopting the technical scheme provided by the invention, the growth of algae is promoted, the culture efficiency of the algae is improved, the problem of proportion imbalance of phycomycetes is relieved, the growth of the algae is effectively promoted, the dissolved oxygen rate of sewage in the barrel body is increased, and the sewage treatment effect is improved.

The invention discloses an environmental climate bionic culture cabin which comprises a cabin body, a fungus culture area arranged in the cabin body and an air conditioning module for controlling the air environment of the fungus culture area. The fungus culture area comprises a culture frame and a culture medium; the air conditioning module is used for simulating an air environment of a fungus origin; the air environment comprises air pressure, temperature, humidity and gas concentration; the air conditioning module is controlled by the central control system; the air conditioning module comprises an air pressure adjusting module, a temperature adjusting module, a humidity adjusting module and a gas concentration adjusting module. The invention aims to accurately simulate the natural environment of the origin of rare fungi through an advanced sensing technology and a real-time data transmission system. Particularly, under the extreme weather condition, the system adopts a multi-stage vacuum pump and a PID control algorithm to quickly adjust the air pressure, temperature lag compensation is achieved through a predictive control algorithm, and it is ensured that temperature change and air pressure change are synchronous.

The invention discloses application of an SFRP1 gene in buffalo ovarian granular cells, and belongs to the technical field of cell engineering and gene engineering. According to the invention, SFRP1 is taken as a research object, an SFRP1 overexpression vector or siRNA-SFRP1 is respectively transfected to ovarian granular cells by constructing the SFRP1 overexpression vector and synthesizing small interfering RNA (siRNA-SFRP1), then changes of signal channel gene mRNA and protein level related to ovarian granular cell apoptosis, proliferation and E2 and P4 secretion are detected, and finally phenotypic changes of the ovarian granular cells are detected. Results show that the SFRP1 can inhibit apoptosis of ovarian granular cells and promote proliferation and E2 and P4 secretion. The application of the SFRP1 in the ovarian granular cells is explored, and the SFRP1 has good application in research of an ovarian follicle atresia mechanism, initial estrus initiation and the like.

The invention discloses a construction and culture method of a glioma organ and a complete culture medium thereof, a glioma tissue sample derived from a patient operation is subjected to tissue treatment processes such as physical cutting, enzymolysis digestion and red blood cell lysis to release a tumor cell cluster, and then the tumor cell cluster is resuspended in a carbon dioxide incubator by using the complete culture medium for shake culture. The tissue treatment process is simple, easy to operate and short in flow, extracellular matrixes such as matrigel do not need to be added in the culture process, the problem that standardization is difficult due to batch difference of the matrigel is solved, and the culture cost is reduced. The self-prepared complete culture medium is clear and simple in formula, and can maintain relatively high proliferation speed and organoid form of the neuroglioma organoid and long-term subculture. The glioma organoid constructed by the method has the advantages of high fitting degree, short culture period, low cost, easiness in standardization, suitability for high-throughput application and the like, and the modeling problem of the glioma organoid is well solved.

The invention discloses a method for culturing seawater rotifers by using prawn shell leavening, which comprises the following steps: S1, cleaning prawn shells to obtain a fermentation substrate; s2, dynamically optimizing fermentation conditions by adopting a multi-agent collaborative reinforcement learning algorithm; s3, predicting the optimal completion time of fermentation by adopting a mixed graph convolutional neural network and an adaptive genetic algorithm, and providing dilution suggestions through a spectral analysis technology; s4, dynamically calculating the dilution ratio, and mixing the fermentation liquor with seawater; s5, pouring the diluent into a rotifer culture pond, predicting the health state of the rotifer by adopting a Vision Transform model, and automatically adjusting a nutrition formula or environmental conditions; s6, monitoring the density of the rotifers in real time through a YOLOv5 target detection model and a camera, and keeping the rotifers within a preset density range; and S7, performing intelligent management on waste treatment based on a multi-objective evolutionary algorithm of non-dominated sorting. According to the method, a multi-agent collaborative algorithm and a deep learning technology are adopted, and intelligent culture of seawater rotifers and optimized treatment of wastes are realized.

The invention belongs to the technical field of plant cultivation, and particularly relates to a wheat shallow-buried drip-irrigation uniform-sowing densification high-yield cultivation method and system, two main treatments of traditional sowing and uniform sowing are set, four different cultivation densities are set at the same time, and changes of wheat growth and yield under different treatments are observed; a drip irrigation tape laying mode of one pipe and two rows is adopted; two chemical control agents of chlormequat chloride and paclobutrazol * mepiquat chloride are sprayed in the three-leaf stage and the elongation stage of wheat, and the dosage of the chemical control agents is set to be three gradients; determining a chemical regulation and control scheme of the spring wheat under the close planting cultivation condition; under the condition of close planting, the control effect of close planting chemical control on wheat growth and yield is studied by adopting a split area test design, and the control effect of water and nitrogen control on wheat growth and yield is studied. According to the method, the planting density and the planting specification of a shallow-buried drip irrigation uniform sowing mode are defined, and a high-yield, high-quality and high-water-efficiency irrigation and fertilization scheme for wheat is determined.

The invention belongs to the technical field of immune cell culture, and particularly relates to an in-vitro amplification culture method of NK cells. The amplification method comprises the steps of CD16 pretreatment, plasma inactivation, lymphocyte separation and multi-stage fluid infusion. The preparation method comprises the following steps: pretreating CD16 in a T75 culture flask, promoting attachment and activation of NK cells, simulating a natural activation state, improving proliferation efficiency, treating whole blood and inactivating plasma to eliminate immunosuppressive factors, reserving nutritional ingredients, supporting amplification of the NK cells, enriching the NK cells through a lymphocyte separating medium, removing impurities and improving purity, mixing an RPMI-1640 culture medium with a solution A, and culturing for 12-24 hours at a high temperature; oK432 and IL-15 are added, the microenvironment is optimized, growth of the NK cells is remarkably promoted, liquid supplementing operation and bottling and bagging are carried out for multiple times, sufficient space and nutrition are provided, the cells are gradually transferred into a serum-free culture medium, and the cell safety and clinical application potential are improved.

The utility model discloses a multi-layer wheat water-saving cultivation device which comprises a cultivation cabinet and cultivation boxes, the cultivation cabinet comprises a cabinet body, and a plurality of supporting plates are arranged in the cabinet body; the cabinet body forms a plurality of cultivation areas through a plurality of supporting plates; a sliding rail is arranged in the cultivation area; a main water pipe is arranged outside the cabinet body, a plurality of branch water pipes are connected to the main water pipe, and the branch water pipes are connected into the cultivation areas respectively and located at the upper ends of the corresponding cultivation areas; the cultivation box comprises a box body, and sliding grooves embedded with the sliding rails are formed in the outer side of the box body of the cultivation box; the multiple cultivation boxes are installed on the supporting plates in the corresponding cultivation areas through the sliding grooves. The cultivation cabinet has the advantages that the interior of the cabinet body is divided into the cultivation areas through the supporting plates, the cultivation boxes are placed in the cultivation areas respectively, the water distribution pipes and the spraying pipes are arranged in the cultivation areas respectively, and the cultivation efficiency can be higher through the multi-layer structure.

The utility model relates to the technical field of culture racks, in particular to an angle-adjustable culture rack. The technical problems that most existing culture racks are fixedly placed in a one-way mode for use, but different types of cells possibly need different illumination, temperature and gas exchange conditions in the culture process, and the culture racks which are inconvenient to adjust can affect the working efficiency are solved. According to the technical scheme, the angle-adjustable culture frame comprises a bearing assembly, a lifting assembly, a fixing assembly, a rotating assembly and a clamping assembly, after a test tube vessel is fixed, a first telescopic sleeve drives the test tube vessel to move upwards to a proper height along a first telescopic column through driving of an air cylinder according to the needed culture height, and in the process, the test tube vessel is fixed through a first telescopic rod; through adjustment of the plug pin, the second telescopic sleeve drives the circular base to move upwards to a proper height along the second telescopic column, and then the plug pin penetrates through the second telescopic sleeve and is in positioning connection with the second telescopic column through the circular hole, so that the test tube vessel reaches a proper height, and culture is facilitated.

The invention relates to the technical field of cultivation devices, in particular to a fruit and vegetable cultivation device which comprises a box body, mounting vertical plates are symmetrically arranged on the box body, a rotary cultivation mechanism for cultivating fruits and vegetables in batches is arranged on the mounting vertical plates, and a plant protection mechanism for preventing a nutrient solution from dripping onto the surfaces of the fruits and vegetables to cause damage is arranged in the rotary cultivation mechanism. A liquid supply mechanism for providing a nutrient solution for the roots of the fruits and vegetables is arranged in the box body, a light source supply mechanism is arranged above the box body, the rotary cultivation mechanism comprises a rotary support movably mounted on a mounting vertical plate, and a driving motor is fixedly mounted on the side, away from the rotary support, of the mounting vertical plate. By arranging the plant protection mechanism, a nutrient solution remaining on the upper open box body in the rotary cultivation process can be prevented from dripping onto the lower open box body through mutual cooperation between a solution receiving cylinder and a bearing assembly, and the phenomenon that the nutrient solution drips onto a fruit and vegetable body to generate adverse effects on the fruit and vegetable cultivation process can be effectively avoided.

The invention discloses an edible mushroom cultivation device and a method thereof, belongs to the technical field of edible mushroom cultivation devices, and aims to solve the problems that after edible mushrooms are generally subjected to a series of pretreatment by adopting mushroom bags, the mushroom bags are put into a cultivation environment to be cultivated, the cultivation quantity each time is generally small, meanwhile, each mushroom bag is not independently separated for cultivation, and the cultivation cost is low. The problems that fungus bags need to be taken out one by one after cultivation, and if a large number of fungus bags need to be cultivated at a time, the workload is large for workers are solved by adopting the fungus bag cultivation device in the prior art, and the problems that when the fungus bags are placed together for cultivation, the fungus bags are prone to being affected, and the cultivation effect is affected. Through cooperation of the box body, the box door, the guide plate, the cultivation plate, the cultivation channel, the baffle and other structures, multiple edible fungus bags can be cultivated at a time, the multiple edible fungus bags can be cultivated in a separated mode, influences of the adjacent edible fungus bags in the cultivation process are avoided, and the cultivation efficiency and effect are improved.

The utility model discloses a mushroom bed earthing and fertilizing device for industrial cultivation of Pleurotus eryngii, which comprises a cultivation mushroom bed, a fertilizing box is arranged in the cultivation mushroom bed, symmetrical guide rail grooves are arranged on the upper surface of the cultivation mushroom bed, symmetrical driving motors are fixedly connected to the outer surface of the fertilizing box, and the driving motors are connected with the fertilizing box. The output ends of the two driving motors are fixedly connected with moving wheels, the two moving wheels are located in the two guide rail grooves respectively, the bottom surface of the fertilization box fixedly communicates with fertilization pipes arranged at equal intervals, the outer surface of each fertilization pipe is provided with a control valve, and the upper surface of the fertilization box is fixedly connected with a water tank. According to the device, the driving motor is matched with the moving wheels, so that the fertilization box can be driven to move stably, the control valve can be used for controlling the outflow of a fertilizer, the plurality of fertilization pipes can be used for uniformly fertilizing a mushroom cultivation bed, and the pump body is matched with the water spraying disc, so that the mushroom cultivation bed can be uniformly sprayed with water, and the effect of timely watering is achieved.

A bin entering and exiting structure belongs to the field of blood culture instruments and comprises a bin body, a driving mechanism, a sliding assembly and a bearing seat, the bin body comprises a bottom plate, the bearing seat is slidably mounted on the bin body through the sliding assembly, the driving mechanism is mounted on the bottom plate, the driving mechanism is in transmission connection with the bearing seat, and the driving mechanism is located below the bearing seat. The driving direction of the driving mechanism is the same as the guiding direction of the sliding assembly. According to the utility model, the driving mechanism is guided by the sliding mechanism to drive the bearing seat to automatically enter and exit from the bin, so that errors caused by manual operation are avoided, the blood culture efficiency is improved, and the bearing seat filled with a blood culture bottle can be pulled out by pulling the transmission belt under the condition of power failure due to belt wheel transmission.

The invention provides a biological culture environment optimization control system and method, and relates to the technical field of biological environment control, and the method comprises the steps: obtaining a target culture stage, reading target predetermined features, carrying out dynamic monitoring to obtain real-time stage features, carrying out screening to obtain target index parameters, inputting a stage classifier, and obtaining a classification result. And when the target plant tissue accords with the predetermined culture constraint, activating a predetermined optimization control strategy, analyzing the predetermined environment demand characteristics to obtain an optimal environment scheme, and performing environment optimization control. The technical problems that in the prior art, due to inaccurate plant tissue culture environment control, the plant tissue growth cycle is prolonged, and the growth efficiency is low can be solved, it is ensured that the plant tissue can grow under the optimal condition in the whole culture process by monitoring the environment conditions in real time and accurately controlling the environment conditions, and the plant tissue culture efficiency is improved. Therefore, the culture efficiency and the growth quality of plant tissues are improved.

The invention belongs to the technical field of biology, and discloses a controllable multi-channel three-dimensional dynamic culture system for simulating a cell mechanical microenvironment, the controllable multi-channel three-dimensional dynamic culture system comprises a driving platform, a flexible driver and a cell culture box, the flexible driver is provided with a planar substrate and an outer wall provided with a periodic protrusion structure, and is provided with a hollow structure serving as an air chamber; one end is connected with the air path module through an air hole channel, and the other end is sealed; a groove is also formed in the outer surface of the planar substrate and is used for accommodating cells to be cultured; the flexible driver air chamber can be pressurized or depressurized, so that the bending degree is enhanced or reduced according to the preset frequency, and a mechanical microenvironment is generated. The three-dimensional dynamic culture system constructed by the invention can simulate a cell mechanical microenvironment, realizes dynamic culture of cells, and promotes cell proliferation and expression of specific proteins. In addition, the system can be conveniently designed into multi-channel culture, and has a very good practical application prospect.

The utility model provides an edible mushroom planting culture dish. The edible mushroom planting culture dish comprises a culture box; the two fixing blocks are both fixedly mounted on the inner wall of the culture box; the culture dish is arranged on the two fixing blocks, and a plurality of draining openings are formed in the bottom of the culture dish; the electric heating wire is fixedly arranged in the culture box and is positioned above the culture dish; the watering mechanism is arranged on the culture box and corresponds to the culture dish; the treatment mechanism is arranged in the culture box and used for treating water and soil lost by watering. The edible mushroom planting culture dish has the advantages that lost culture soil can be collected in a centralized mode and recycled, and the planting cost of edible mushrooms is reduced.

The invention provides a mesenchymal stem cell preparation as well as a preparation method and application thereof, and relates to the technical field of biology, the mesenchymal stem cell preparation contains 5 * 10 < 6 >-1.2 * 10 < 7 > cells / mL of mesenchymal stem cells, 0.1-0.6% w / v of human serum albumin and a solvent; the mesenchymal stem cells are obtained by culturing a serum-free culture medium, and the mesenchymal stem cells are obtained by resuscitating cryopreserved mesenchymal stem cells, inoculating the resuscitated mesenchymal stem cells into a culture container, culturing until the mesenchymal stem cells are completely adhered to the wall, and digesting. The mesenchymal stem cell preparation improves the treatment effect of mesenchymal stem cells.

The invention discloses a cell culture container which comprises a container body, a connecting plate is arranged in the container body, and a plurality of through holes for cell sap to pass through are distributed in the connecting plate. The invention further discloses a shaking table which comprises a movable platform, a platform driver and the cell culture container, the container body is arranged on the movable platform, and the platform driver is used for driving the movable platform. The cell culture container and the shaking table have the advantages of being suitable for large-scale culture, capable of timely and uniformly stirring cell sap and the like.

The invention relates to the technical field of fungus material culture, and particularly discloses a fungus material culture method of armillaria mellea for artificial cultivation of gastrodia elata, which comprises the steps of fungus material selection, culture medium preparation, inoculation, culture shelf arrangement, environment arrangement, harvesting and gastrodia elata planting. According to the method, hard and corrosion-resistant broad-leaved tree species are selected as the fungus material, a composite culture medium formula is adopted, namely, the advantages of a liquid culture medium and a solid culture medium are combined, comprehensive and balanced nutrition support is provided for growth of armillaria mellea, the culture efficiency and quality of the fungus material are improved, meanwhile, the proportion of the formula of the culture medium is designed, and the culture cost is reduced. The water content and the pH value of the culture medium are controlled, favorable growth environment conditions are created for growth of armillaria mellea, meanwhile, a syringe or an inoculating loop for high-pressure steam sterilization effectively prevents contamination of infectious microbes, and the success rate of inoculation and the purity of hyphae are improved.

The invention relates to the technical field of cell biology, and particularly discloses a goat embryonic stem cell culture system, a system establishment method and application, the goat embryonic stem cell culture system takes mTeSRTM Plus as a basic culture solution, and 20 ng / mL to 25 ng / mL of leukemia inhibition factors, 1 mu M to 1.5 mu M of PD0325901, 4 mu M to 5 mu M of IWR-1, 45 mu g / mL to 50 mu g / mL of Vc, 15 ng / mL to 20 ng / mL of activin A, 1 mu M to 1.2 mu M of CGP77675 and 1.5 mu M to 2 mu M of GO6983 are added. Cell clone with clear edges is obtained through culture of a goat embryonic stem cell culture system, stable passage can be achieved, and the cell clone has the potential of being differentiated into three germ layers.

The invention discloses a culture medium and a culture method of appendix mucinous tumor organoid. The culture medium of the appendix mucinous tumor organoid is prepared by adding the following components with final concentration into a basic culture medium: 2-8nM of NRG1 and 1-2mu g / ml of R-Spondin1, 90 to 110 [mu] g / ml of Noggin; 70 to 80 [mu] g / ml of Wnt-3a; eGF with a concentration of 45 to 55 ng / ml; the concentration of the FGF-10 is 90 to 110 ng / ml; 1X B to 27TM; 4 to 6 mM of nicotinamide; 4 to 6 nM of prostaglandin E2; 9 [mu] g / ml to 11 [mu] g / ml of Transferrin human; 4-6 [mu] g / ml of insulin; 4 to 6 nM of gastrin I; 9 to 11 [mu] M of Y27632; 1 to 2 [mu] M of A83-01; 4 to 6 [mu] M of SB202190; and 2 to 3 [mu] M of CHIR999021. The culture medium is high in induction rate.

The utility model belongs to the technical field of injection liquid culture boxes, particularly relates to a culture box for injection liquid production, and aims to solve the problems that the internal humidity of a box body cannot be adjusted and the adjustment convenience of a culture environment and the survival rate of cultures are reduced due to the fact that existing equipment is not provided with a humidifying part. A water inlet pipe is welded to one end of the water pump, a water outlet pipe is welded to the bottom end of the water pump, the bottom end of the water outlet pipe extends and penetrates through the water tank, and evenly-distributed pipes are symmetrically welded to the bottom end of the water outlet pipe through welding. A plurality of atomizing nozzles are uniformly mounted at the bottom end of the uniform distribution pipe through screw holes, ultraviolet lamps are symmetrically mounted at the bottom end of the water tank through bolts, and ventilation openings are formed in the tops of the two ends of the equipment box through welding. The plurality of atomizing nozzles are arranged at the top of the incubator, so that atomized water vapor is uniformly distributed, and the culture efficiency and the survival rate of substances in the incubator are improved.

The utility model relates to the technical field of microorganism culture, in particular to a microorganism constant-temperature incubator which comprises a base, an incubator main body and two sealing doors, the two sealing doors are movably connected to the two sides of the front end of the incubator main body through hinges, and a microorganism stable placement structure is arranged at the lower end of the inner side of the incubator main body. A microorganism stable heat supply and constant temperature keeping structure is arranged at the upper part in the incubator main body. According to the microorganism constant-temperature incubator, a microorganism culture solution is directly put into the microorganism culture dishes, then the sealing door at the front end of the incubator main body is opened, the bearing plate and the microorganism culture dishes above transversely slide into the incubator main body, and then the sealing door is closed; due to the transverse limitation of the limiting groove, the bearing plate can only drive the microorganism culture dish above to move to a designated position to be placed, and the subsequent microorganism culture process does not shake or have more other interference items, so that the microorganism culture process is not easily influenced by the microorganism constant-temperature incubator.