The invention discloses special biological organic fertilizer for cucumbers and a preparation method of the special biological organic fertilizer, which belong to the technical field of organic fertilizers. The fertilizer is prepared from cattle and sheep excrement, corn straws, peanut seedlings, hazelnut meal, humic acid, brown sugar, EM fungicide, rhizoma coptidis, honeysuckle flower, Houttuynia cordata, radix isatidis, urea, trace elements, attapulgite, sepiolite powder, soil conditioner and a composite microorganism agent according to a given weight proportion. The fertilizer has rich nutrients, satisfies the nutrient demand for the growth of the cucumbers at different periods, can effectively improve the physical and chemical properties of the soil, can improve the water and fertility retention capacity of the soil, can prevent various physical and chemical diseases of plants, and can effectively improve and the yield and quality of the cucumbers.