84 results about "Fertility" patented technology

The invention relates to a male gamete killer gene S19g-A1 of a rice interspecific hybrid sterile S19 seat and application of the male gamete killer gene S19g-A1. The invention provides a rice hybrid sterility related protein S19g-A1. The amino acid sequence of the rice hybrid sterility related protein S19g-A1 is as shown in SEQ ID NO. 2. According to the invention, a key gene S19g-A1 of a rice interspecific hybrid sterile S19 site is researched and identified, function knockout is carried out on African rice S19g-A1, and pollen fertility of a mutant hybrid F1 obtained by carrying out distant hybridization on an obtained African rice s19g-a1 mutant returns to normal. Based on a gene editing technology, the invention develops a method for rapidly creating a hybrid affinity line capable of overcoming sterility of African rice hybrid, effectively eliminates S19 locus mediated interspecific hybrid reproductive disorder, breaks through the bottleneck of rice interspecific hybrid advantage utilization, provides target gene resources for distant hybrid advantage utilization and crop yield improvement, and has a wide application prospect. Good application prospects are realized.

The invention provides a double-cropping rice field annual rotation fertility dynamic monitoring system based on the Internet of Things, and relates to the technical field of fertility monitoring, and the system comprises a sensing collection module which is used for collecting soil fertility under the annual scale, and synchronously recording double-cropping rice growth indexes and rotation management information; the data processing module is used for preprocessing the multi-dimensional original monitoring data flow and generating a structured data packet; the trend prediction module is used for predicting fertility change through a Prophet algorithm; and the scheme optimization module is used for constructing a fertility regulation and control model and generating an optimized fertility regulation and control scheme. According to the method, the Prophet algorithm is used for feature extraction and model training, so that accurate pre-judgment and early warning of fertility change are realized; the fertility regulation and control rule base is constructed based on the prediction result and the rotation tillage characteristics, personalized regulation and control schemes are generated according to the risk levels of different field parcels, the scheme effect is optimized through historical scene verification, and the pertinence and the implementation effect of fertility regulation and control are effectively improved.

This invention belongs to the field of molecular genetics and discloses a method and application for controlling fish fertility using in vivo gene editing technology. The applicant, for the first time, cloned a carp-specific U6 promoter and used this promoter to construct the U6 gene. MOVIE gRNA transgenic vectors and water The Cas9 vector allows for the establishment of an in vivo editing system. This strategy enables the heritability of sterility, a reproductive control trait, through hybridization of fertile parents to produce sterile offspring. The operation is highly efficient, simple, and environmentally friendly. Furthermore, since high-copy-rate short, scattered repetitive sequences exist in all fish species, this strategy has broad applicability across species.

The invention relates to the technical field of animal husbandry, in particular to a synchronous oestrus-timing insemination method suitable for cattle, which comprises the following steps: firstly, performing intramuscular injection of PG (cloprostenol sodium) at different time points after calving to promote uterine recovery and luteolysis and lay a synchronous oestrus foundation; the method comprises the key steps that GnRH (gonadotropin release hormone) is given through intramuscular injection on the 43rd day, the 53rd day, the 60th day and the 69th day, pituitary is promoted to release follicle-stimulating hormone (FSH) and luteinizing hormone (LH), collection and screening of follicles are guided, follicle maturation is promoted, the oestrus uncovering rate and the oestrus conception rate are increased, a melatonin implant is implanted in a subcutaneous implantation mode on the 63rd day, and the melatonin implant is implanted in a subcutaneous implantation mode on the 63rd day. The synthesis of ovarian hormones is promoted by regulating the hypothalamus-pituitary-gonad axis, the secretion of follicle cell factors and maturation promoting factors is promoted, the development of granular cells is promoted, and the maturation of oocytes is promoted. According to the method, the pregnancy rate of a double-synchronization-timing insemination scheme which is most widely used in a large-scale dairy cow breeding pasture at present can be remarkably increased, and feasible technical support is provided for success of increasing the breeding efficiency of cows.

The invention discloses an application of an overexpression level transfer gene GlpQ in improving the fertility of male silkworms, and the nucleotide sequence of the GlpQ is shown as SEQ ID NO.3. After the GlpQ1 gene is overexpressed, the reproductive activity of the male silkworms is obviously enhanced, the number of female silkworms capable of mating with each male silkworm on average is obviously increased, and the reproductive activity of the male silkworms is obviously improved. Correspondingly, the number of female silkworms capable of being fertilized by the male silkworms is also obviously increased, the testis development process can be promoted, the gene may act on the testis outer membrane to promote the increase of the testis volume and act on the seminal vesicle to promote sperm formation, and therefore the gene can be used for the fertility of the male silkworms.

This invention discloses a method for constructing a fast-growing, sterile zebrafish model, belonging to the field of fish animal model preparation technology. This invention is the first to discover the role of the CK gene in regulating fish growth rate and fertility: fish with simultaneous knockout of ck1 and ck2 genes exhibit rapid growth and gonadal abortion. Using the method provided by this invention, a fast-growing, sterile zebrafish model was successfully constructed. These zebrafish exhibited gonadal dysplasia and sterility, but also rapid growth. Compared to wild-type zebrafish, the 4-month-old double mutant zebrafish showed significantly increased body length and weight. The disclosed gene function and zebrafish model construction method can be used for breeding fast-growing, sterile fish varieties and studying related mechanisms, which is of great significance for improving the economic benefits of aquaculture and protecting ecological security.

The invention discloses a method for evaluating damage of ionizing radiation to male sperm quality, which belongs to the technical field of reproductive biology, and comprises the following steps: establishing a dose-effect database model between ionizing radiation dose and male sperm quality based on a logistic regression model, determining a mapping relation between sperm quality rating and fertility recovery average time; collecting a to-be-detected semen sample exposed by ionizing radiation, comparing the to-be-detected semen sample with the dose-effect database model, evaluating the male sperm quality rating, and predicting the fertility recovery time based on the male sperm quality rating and the mapping relation.

A method for providing assistance in determining the fertility of a female subject according to the present invention comprises a step for generating information for determining the fertility of the female subject by using the proportion of cells in which an X chromosome has been lost in somatic cells collected from the female subject.

The present invention relates to the use of vitamin D3, 25-OH-D3 and / or 25-OH-D3 and canthaxanthin to improve fertility in birds, preferably poultry, more preferably laying hens. The invention also relates to a method for improving the fertility of birds. The method comprises a feeding regimen and a feed adaptive to the age of the birds.

The present application relates to the field of agricultural technology, in particular to a plant temperature-sensitive male sterility gene mutant tms17 and application thereof.The nucleotide sequence of the plant temperature-sensitive male sterility gene mutant tms17 comprises the nucleotide sequence shown in SEQ ID NO.1 in the sequence listing.The present application screens a new temperature-sensitive male sterile line tms17 by chemical mutagen ethyl methyl sulfone (EMS) mutagenesis of japonica rice variety ZH11, which is completely sterile at high temperature and fertility is restored at low temperature, and the mechanism of temperature-sensitive fertility restoration is disclosed, which provides a new germplasm resource with application potential for rice two-line hybrid breeding.

Embodiments of the invention comprise a test strip configured to detect one or more metabolites of progesterone in urine, and optionally further detect one or more additional metabolites or hormones in urine within the same test strip. Embodiments of the invention are methods of utilizing such a test strip in association with avoidance of pregnancy, detection of menopause, and in association with fertility planning purposes. Embodiments of the invention are associated with the incorporation of a test strip within a testing kit, optionally further comprising a digital reader.

The present invention is directed to methods and uses of a series of compounds and compositions comprising the same for treating diseases, disorders and / or conditions related to fertility and preserving same and conditions related to same. In some aspects, the compounds and or compositions as herein described promote, improve, recover or restore fertility in a subject in need thereof, including in male and / or female subjects. Such methods / uses include promoting, enhancing or improving fertility-associated cell or tissue yield as part of an in vitro fertilization protocol.

Disclosed are methods and products for improving the quality of sperm, methods for treating subjects to improve sperm quality or improve fertility, and use of sperm with improved quality in assisted reproductive technologies, including exposing the sperm to BGP-15 and / or a derivative thereof, such as BGP-15, propanolol, bimoclomol, arimoclomal, NG94, iroxanadine, and / or a pharmaceutically acceptable derivative, prodrug, solvate, salt, tautomer, stereoisomer, and / or racemate thereof.

The invention discloses a gene BnA09GDSL for regulating and controlling male sterility of brassica napus and application of the gene BnA09GDSL. According to the invention, a novel gene BnA09GDSL for regulating and controlling the male sterility of the brassica napus is identified, a PC3301-BnA09GDSL overexpression vector and a CRISPR-BnA09GDSL gene editing vector are constructed, the gene BnA09GDSL is overexpressed in a male sterility line Bngms1, and the plant fertility is recovered; meanwhile, a CRISPR-BnA09GDSL transgenic plant is obtained, and the transgenic plant has a male sterility phenotype. The BnA09GDSL gene obtained by cloning can regulate and control the fertility of the brassica napus, so that cross breeding is facilitated, and the BnA09GDSL gene has important application value in the field of male sterility breeding of the brassica napus.

Provided herein are methods and compositions comprising pulsatile follicle stimulating hormone (FSH). The pulsatile FSH may be used in methods of treating infertility, restoring normal hormonal levels, stimulating secretion of a sex steroid, treating or preventing fatty tissue accumulation, treating or preventing loss of bone density, preventing ovarian inflammation, and / or promoting metabolic health.

The application belongs to the field of genetic engineering, and discloses a maize pollen fertility regulation gene ZmPUB33 and an encoded protein. The gene regulates maize pollen aperture development and further mediates pollen fertility ability. After mutation, the gene cannot normally blossom, anther husks cannot basically crack, anthers are smaller than wild types, and almost not exposed, pollen grains are not active, and can cause complete male sterility of plants. The sterile line can be used for sterile seed production and production of hybrid crops, and has great application and economic value.

The invention relates to a method for increasing the lifespan of animal sperm comprising contacting said sperm with an inhibitor of Slo3 potassium channel.The invention also relates to a use of an inhibitor of Slo3 potassium channel, for increasing the lifespan of animal sperm or motility of capacitated animal sperm, comprising contacting an inhibitor of Slo3 potassium channel with said sperm. Moreover, the invention relates to an artificial insemination instrument for use in artificial insemination of an animal, comprising animal sperm in contact with an inhibitor of Slo3 potassium channel.The invention also relates to a method for artificially inseminating an animal using said artificial insemination instrument.Eventually, the invention relates to a method for increasing the fertility of an animal, comprising contacting sperm of said animal with an inhibitor of Slo3 potassium channel; then artificially inseminating said animal with said sperm.

The invention provides a plant male gamete fertility control method and a method for obtaining a male sterile material, and belongs to the technical field of plant breeding. According to the invention, the PPCD1 is taken as a target gene, and the PPCD1 gene is subjected to expression inhibition or gene knockout, so that the PPCD1 gene loses functions, pollen development fails, and the purpose of fertility control is achieved. Therefore, the male sterility material can be created through the method, and the method can be used for creating male sterility materials in cross breeding, creating materials for preventing genetic escape of transgenic plants, creating plant materials without pollen pollution and the like, so that the production efficiency, the economic effect and the social and environmental effects are greatly improved.

The invention provides application of ergothioneine in improving fertility of high-fertility-age female individuals, and belongs to the technical field of natural medicines. The invention provides an application of EGT in preparation of a product for improving fertility of a high-fertility-age female individual. In the embodiment of the invention, a high-fertility-age ICR female mouse is taken as an animal model, the influence of the EGT on the fertility of the high-fertility-age female mouse is verified from in-vivo and in-vitro aspects, and the result shows that the EGT has the effect of improving the in-vitro fertilization rate and blastocyst rate of oocytes of the high-fertility-age female mouse; the superovulation number and the litter number of high-childbearing-age female mice are increased. It is proved that the EGT has the effect of improving the fertility of high-fertility-age female individuals.

The invention discloses a novel application of a compound beta-alanine (beta-alanine) in preparation of a medicine for improving oocyte quality and treating ovarian endometriosis (OEM) related infertility, and belongs to the technical field of biological medicines. An OEM animal model proves that beta-alanine can remarkably improve reproductive function disorder of model animals, specifically, follicular dysplasia caused by endometriosis is effectively relieved, the fertility is improved, and the litter size is increased; in the aspect of oocyte quality, the compound can promote normal discharge of a first polar body in an oocyte in-vitro maturation process, reduce cytoplasm fragmentation, recover spindle body form and microtubule arrangement, enhance mitochondrial membrane potential stability and maintain the integrity of an ultrastructure. The results show that beta-alanine can reverse oocyte quality reduction caused by ovarian endometriosis in multiple dimensions, has a definite treatment potential, and provides an important candidate molecule for developing a novel assisted reproduction intervention strategy for targeting oocyte health.

This disclosure concerns methods and compositions for identifying plants that have an Ogura CMS restorer of fertility phenotype. Some aspects concern a zygosity assay to identify, select, and / or construct Ogura CMS restorer of fertility plants and germplasm. Particular aspects concern a zygosity assay to maintain an Ogura fertility restoration system for hybrid canola production. This disclosure also concerns plants comprising an Ogura fertility restoration system for hybrid canola production generated by methods utilizing the zygosity assay described herein.

This invention provides a molecular marker 27W1 for breeding high-fertility Litopenaeus vannamei populations and its applications. The nucleotide sequence of the molecular marker 27W1 is shown in SEQ ID No. 1, wherein the allele frequency of G at base 301 in high-fertility Litopenaeus vannamei populations is ≥98.33%. This invention also provides primers for the molecular marker 27W1, including amplification primers with nucleotide sequences shown in SEQ ID No. 2 and SEQ ID No. 3, and extension primers with nucleotide sequences shown in SEQ ID No. 4. The high-fertility molecular marker 27W1 for Litopenaeus vannamei can be used for early breeding of Litopenaeus vannamei, regardless of growth stage, to rapidly select parent populations with excellent reproductive traits and promote the breeding process of new high-fertility Litopenaeus vannamei varieties.

The application provides a method and system for fertility guidance based on deep learning image recognition. The method comprises: obtaining environmental data by a pre-set test paper collection module, determining whether the environment is qualified, starting the focusing auxiliary function, obtaining test paper image data and uploading to the cloud after obtaining the test paper image data, and obtaining effective test paper image data after the cloud server processes the test paper image data; obtaining a test paper result quantitative value by weighting calculation according to a test paper quantitative parameter; obtaining a test paper detection state by threshold comparison of the test paper result quantitative value; thereby improving the accuracy of test paper detection state evaluation through the judgment of the collection environment state, the calculation of the test paper result quantitative value and the threshold comparison.

This invention provides GhFRC1 The application of genes in regulating plant fertility belongs to the field of plant genetic engineering technology. This invention is the first to clone genes from cotton. GhFRC1 The gene was identified and verified to be heterologously expressed in three different genera and families of crops: Arabidopsis thaliana, tomato, and rice, and to play a role in fertility regulation. This breakthrough overcomes the limitation of existing fertility genes that can only regulate the fertility of a single crop, and realizes "broad-spectrum regulation of fertility of multiple crops by a single gene", filling the gap in the application of distant heterologous expression of cotton fertility genes. GhFRC1 After heterologous expression of the gene, it can stably lead to inactive pollen and abnormal fruit set in the recipient crop, and can also cause a phenotype of complete pollen sac non-development. Moreover, the sterility trait can be restored to fertility through normal pollen pollination. This solves the problem of the narrow applicability of existing fertility genes and the great limitation of their breeding applications, and provides new gene resources and technical solutions for fertility regulation of multiple crops and hybridization breeding.

This invention belongs to the field of plant molecular breeding, and specifically discloses a gene related to rice fertility restoration. OsRf19 This invention addresses the cumbersome process of identifying restorer lines for the existing Fujian abortive cytoplasmic male sterility (CMS-FA / OsRf19) system by developing a method for creating tightly linked artificial haplotypes, the development of InDel molecular markers, and their application in rice fertility. Through bioinformatics analysis, this invention identifies a fertility restoration gene associated with the rice sporophytic sterility system CMS-FA. OsRf19 A specific region closely linked to the PPR gene cluster and with extremely low similarity to the loci was edited using CRISPR / Cas12a gene editing technology, resulting in multiple large deletions. Based on these genotype sequence variations, a set of InDel molecular markers was developed to realize the editing of this artificially created restoration gene. OsRf19 Rapid identification of closely linked artificial haplotypes accelerates the breeding process of molecular marker-assisted selection in the rice CMS-FA system.

This invention belongs to the field of family planning, specifically relating to a sterile fertility-protecting condom suitable for pregnant women and its preparation method. The preparation method includes: medical-grade natural latex undergoing sequential deproteinization, purification, and defoaming treatments; then, it is molded and vulcanized to obtain a semi-finished condom; sodium hyaluronate is mixed with purified water to obtain an aqueous lubricant; the aqueous lubricant is coated onto the semi-finished condom, dried, and then sterilized by electron beam irradiation to obtain a sterilized semi-finished condom; the sterilized semi-finished condom is tested, and if qualified, packaged and stored to obtain the finished sterile fertility-protecting condom suitable for pregnant women. This invention solves the problems of traditional silicone oil condoms damaging sperm and sodium hyaluronate being intolerant to radiation, thus preventing unintended pregnancy while protecting fertility.

The invention belongs to the field of gene engineering and molecular breeding, and particularly discloses interaction protein MORF8 of rice wild abortion type cytoplasmic male sterility recovery protein Rf4 and application of the interaction protein MORF8, the amino acid sequence of the interaction protein MORF8 is shown as SEQ ID NO.2, the interaction protein MORF8 is coded by an MORF8 gene, and the nucleotide sequence of the MORF8 gene is shown as SEQ ID NO.1; the application of the interaction protein MORF8 of the rice wild abortion type cytoplasmic male sterility restoring protein Rf4 in rice breeding comprises the following steps: constructing a rice wild abortion type cytoplasmic male sterility line overexpressing the MORF8, and hybridizing the rice wild abortion type cytoplasmic male sterility line overexpressing the MORF8 with a rice wild abortion type cytoplasmic male sterility restoring line containing the restoring protein Rf4, hybrid seeds which synergistically enhance the fertility recovery capability of the recovery protein Rf4 are generated. Compared with the prior art, the invention discloses a synergistic enhancement function of MORF8, obviously improves the maturing rate of rice, and provides an efficient and safe novel molecular breeding tool for creating high-yield and stable-yield hybrid rice.

The application discloses a pepper cell nucleus male sterility molecular marker and application thereof. The pepper cell nucleus male sterility molecular marker disclosed by the application is a DNA fragment corresponding to sequence 3 in a sequence list in a pepper genome, and the pepper genome contains or does not contain the DNA fragment of sequence 3 in the sequence list. Experiments prove that in a pepper 20Q5066AB population of 554 strains, there are 281 fertile strains, all of which are fertile in field fertility identification and all contain the DNA fragment shown in sequence 3 in the sequence list; there are 273 sterile strains, all of which are male sterile in field fertility identification and all do not contain the DNA fragment shown in sequence 3 in the sequence list. It is shown that the pepper male sterility molecular marker of the application can be used for identifying pepper male sterility, has very strong applicability, and can be used for fertility rapid identification in practical breeding application.

The invention provides application of a reagent for detecting KAT5 or H2A.Zac in preparation of a product for diagnosing placenta-derived diseases, and relates to the technical field of biomedicine. Experimental research of the inventor finds that chromosomes of trophoblasts in placentas of patients with repeated pregnancy loss are unstable, and expression of KAT5 and H2A.Zac is remarkably reduced. Experiments prove that KAT5-mediated H2A. Zac reduction is a main reason for reduction of trophoblast chromosome stability, so that the KAT5-H2A. Zac pathway provides a new action target for diagnosis and treatment of placenta-derived diseases, diagnosis and / or treatment of placenta-derived diseases can be realized, a new treatment strategy is provided for clinical improvement of poor pregnancy outcome, and the application of the KAT5-H2A. Zac pathway in diagnosis and treatment of placenta-derived diseases is developed. Therefore, the health level of female fertility can be improved, and gestation complications such as abortion and fetal growth limitation can be reduced.