280 results about "Functional protein" patented technology

The invention provides a plug-and-play Mi3 protein nanocage fusion protein with a VNP6 tag and a preparation method and application of the plug-and-play Mi3 protein nanocage fusion protein, the fusion protein comprises the VNP6 tag, a SpyCatch003 component, a Mi3 protein nanocage and a functional protein sequence, and the VNP6 tag is located at the N end of the fusion protein. The VNP6 peptide sequence or the variant thereof is utilized to induce a vesicle or vesicle-like structure beneficial to protein folding and stabilization in escherichia coli, so that the correct folding rate and activity of recombinant protein are improved while the overall intracellular molecular crowding effect is relieved. The core idea of the invention lies in that the internal structure of cells is regulated at low temperature, and the internal microenvironment of an escherichia coli expression system is optimized, so that protein molecules under high expression load can obtain more reasonable spatial distribution, and the problems of misfolding and aggregation caused by molecular crowding are reduced.

A method for functional antimicrobial peptide design is provided. The method includes running pretrained protein large language models as a generator and enhancing a sample candidate. The enhancing a sample candidate includes performing an automatic pipeline based on machine learning methods and a plurality of bioinformatics methods and is configured to perform protein inverse folding and computational protein sequence designing. The computational protein sequence designing includes running a pretrained deep learning-based protein structure model, an autoregressive pretrained protein sequence model, and a deep learning-based alignment model. The pretrained deep learning-based protein structure model is configured to learn three-dimensional structures of the proteins and output latent structure embeddings in a high dimensional space. The autoregressive pretrained protein sequence model includes a protein language model to automatically generate protein sequences and is trained to predict next amino acid in a protein sequence based on a preceding sequence(s) of amino acids.

The invention discloses a sea cucumber flower self-assembled polypeptide-selenium nano-carrier with antioxidant activity and a preparation method and application thereof, belongs to the technical field of functional protein peptides, and particularly relates to a preparation method of the sea cucumber flower self-assembled polypeptide-selenium nano-carrier with antioxidant activity, which comprises the following steps: heating a sea cucumber flower protein solution; the preparation method comprises the following steps: mixing sea cucumber with simulated gastric juice, reacting for 30-150 minutes, then adding simulated intestinal juice, reacting for 20-120 minutes, carrying out enzyme deactivation treatment, centrifuging, taking supernate, and freeze-drying to obtain sea cucumber flower self-assembled polypeptide; and mixing the sea cucumber flower self-assembled polypeptide with deionized water to prepare a sea cucumber flower self-assembled polypeptide solution, mixing the sea cucumber flower self-assembled polypeptide solution with a sodium selenite solution and a phosphate buffer solution, reacting for 20-60 minutes, washing with an ethanol solution, centrifugally collecting precipitate, and freeze-drying to obtain the sea cucumber flower self-assembled polypeptide-selenium nano-carrier. The sea cucumber flower self-assembled polypeptide-selenium nano-carrier prepared by the invention has good antioxidant activity and potential of effectively delivering selenium to an organism.

The invention discloses a recombinant protein and application thereof in preparation of sodium alginate fibers. The recombinant protein is obtained by fusion expression of a functional protein and at least one alginic acid binding domain protein. The amino acid sequence of the alginic acid binding domain protein is as shown in at least one of SEQ ID No. 1, SEQ ID No. 2 and SEQ ID No. 3. The recombinant spider silk protein with the alginic acid CBM region can exert the material science characteristics in a sodium alginate-calcium ion system, the mechanical property of alginic acid fiber is greatly enhanced, and meanwhile, the biological activity of protein can be provided for an alginate fiber system.

Embodiments disclosed herein provide methods, including computer-implemented methods, for designing guide sequence which may be incorporated into custom, large scale guide sequence libraries. The methods require only a list of target genes as input and utilize on target and off target scores to generate an optimal set of guide sequences for a set of target genes. In certain embodiments, the methods may also utilize multi-tissue RNA-sequencing data and / or protein annotation to design targets to genes that are highly expressed and / or contain a functional protein domain. The invention further comprises guide libraries, cells comprising said guide libraries. Computer-implemented embodiments further improve computer system function by reducing excessive user wait time through the use of data structures that reduce search from linear to logarithmic time.

A construction and use of an environmentally adaptive co-regulated mRNA nanodelivery system. By incorporating EACR molecules into mRNA nanoparticles, the microenvironment is remodeled to be suitable for robust and sustained mRNA expression, while tissue damage associated with the self-immunogenicity of mRNA drugs is avoided. The nanodelivery system is compatible with ionizable lipid nanoparticles, cationic liposomes, cationic nanoemulsions, and polymeric nanoparticles. The EACR molecules include: anti-inflammatory drugs, tyrosine kinases / adaptors, JAK / STAT and MAPK pathway inhibitors, nutrients and metabolites, membrane transporters / ion channels, phosphodiesterase and cellular stress inhibitors, and natural viral proteins. The therapeutic mRNAs may encode tumor, viral, or bacterial antigens, immunomodulatory factors, therapeutic antibodies, or functional proteins / enzymes, and can play a role in the fields of regenerative medicine, protein supplementation / replacement therapy, targeted gene editing, and immunotherapy.

The invention discloses a functional protein screening method based on a large language model, and belongs to the technical field of protein screening, and the method specifically comprises the following steps: obtaining a protein three-dimensional structure and functional literature, converting the protein three-dimensional structure and functional literature into a multi-modal feature vector by using an encoder, and constructing a domain-specific vector database; after a screening instruction is received, related feature data of semantic matching is called through a vector retrieval algorithm; then, inputting the data into a large language model to execute biological mechanism reasoning, and outputting an initial candidate protein sequence containing predicted activity; further, physical binding energy is calculated by using a molecular docking program, a deviation coefficient between the physical binding energy and the predicted activity is generated, and the coefficient is used as a penalty term to construct a dynamic correction function to update the scoring weight; and finally, performing secondary sorting on the candidate set based on the updated weight, and outputting the sequence sorted for the first time as a target protein. According to the method, AI reasoning and physical verification are effectively combined, and the screening accuracy is improved.

The invention discloses a scorpion-derived polypeptide and application thereof, and belongs to the technical field of functional proteins. The amino acid sequence of the polypeptide derived from the Buthus martensii Karsch is as shown in SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4 or SEQ ID NO. 5. The polypeptide derived from the Buthus martensii Karsch is a polypeptide derived from the Buthus martensii Karsch. The invention also provides an application of the buthus martensii-sourced polypeptide with the amino acid sequence shown as SEQ ID NO. 1 in preparation of a blood coagulation promoting drug and an application of the buthus martensii-sourced polypeptide with the amino acid sequence shown as SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4 or SEQ ID NO. 5 in preparation of a blood coagulation promoting drug. The buthus martensii-sourced polypeptide provided by the invention has a relatively good application value in the aspect of research and development of antithrombotic drugs or coagulant drugs.

The invention relates to the technical field of functional protein extraction, in particular to a mulberry leaf protein extraction method and application thereof. The extraction method comprises the following steps: dissolving mulberry leaf powder in water, and adjusting the pH value to be greater than or equal to 8 to obtain a mulberry leaf powder aqueous solution; carrying out digestion after ultrasonic crushing, and centrifuging to take supernatant as a crude extract; and adjusting the pH value of the crude extract to be less than or equal to 6, and centrifuging to take the precipitate. The method for extracting the mulberry leaf protein is provided by combining an alkali dissolution method and an ultrasonication method, the extraction rate of the mulberry leaf protein and the oxidation resistance of the extracted mulberry leaf protein can be effectively improved by combining the alkali dissolution method and the ultrasonication method, and the method has important application value for functional development of the mulberry leaf protein.

The invention discloses a rice grain type protein Os03g0762000 as well as a coding gene and application thereof. The rice grain type protein Os03g0762000 has an amino acid sequence as shown in SEQ ID NO.2, or is a functional equivalent variant formed by substitution, deletion or addition of one or more amino acid residues in the sequence as shown in SEQ ID NO.2; the coding gene has a nucleotide sequence as shown in SEQ ID NO.1, or a nucleotide sequence which is hybridized with SEQ ID NO.1 and codes the same functional protein. The invention discloses a method for cultivating a small-grain high-quality variety by using the gene, namely editing a specific position of the gene by a gene editing method so as to obtain a small-grain rice germplasm. The regulatory gene is good in regulation effect, compared with a wild type, the rice grain length and grain width are obviously reduced, and the characteristics of the regulatory gene show unique application value in rice mechanized hybrid breeding.

The invention discloses a mechanical response type engineered mesenchymal stem cell exosome preparation for treating diseases caused by fibrosis inflammation, a preparation method and application, and the exosome preparation is an exosome which is secreted by mesenchymal stem cells and has high cholesterol and high-efficacy protein. The exosome is prepared by culturing mesenchymal stem cells on a hydrogel matrix in a modulus hardness range of 1-50KPa, the exosome secretion amount of a single cell is (3-5) * 10 < 4 > / cell, the cholesterol content is (1-10) nM / 5mg exosome, and the size of the exosome is 110-140nm; the high-efficacy protein is a key functional protein for regulating and controlling the biological activity of the exosome and the cell action efficiency. The cholesterol content in the exosome is precisely regulated and controlled, the cytoplasm delivery efficiency and tissue penetrability of the exosome are improved, a whole-process control system of hydrogel preparation, stem cell culture and exosome purification is constructed, and the stability of the preparation and the yield of the exosome are improved.

The invention provides chickpea protein peptide as well as a preparation method and application thereof. The preparation method of the chickpea protein peptide provided by the invention comprises the following steps: sequentially carrying out enzymolysis on chickpea protein isolate by using neutral protease, pepsin and pancreatin; after the enzymolysis is finished, performing ultrafiltration on an enzymolysis product by using an ultrafiltration membrane with the interception amount of 3kDa, and collecting components with the molecular weight of less than 3kDa; and finally, carrying out gel chromatography on the component, and collecting the corresponding component to obtain the chickpea protein peptide. The chickpea protein peptide prepared by the method has inhibitory activity on DPP-IV, alpha-glucosidase and alpha-amylase, can effectively inhibit the activity of Caco-2 cell sucrase, maltase and DPP-IV, and has a certain inhibitory effect on glucose transport; therefore, the chickpea protein peptide provided by the invention has good hypoglycemic activity, and a new thought is provided for development of food-borne functional protein peptides.

Disclosed herein are computer-implemented systems and methods for generating functional protein sequences using a library of protein fragments.

The invention relates to the technical field of gene engineering, in particular to an atractylodes macrocephala terpene synthase gene AmTPS9 and a product coded by the same and application of the gene AmTPS9. The gene AmTPS9 has one of the following nucleotide sequences: (1) a nucleotide sequence as shown in SEQ ID NO.1; and (2) a nucleotide sequence which is obtained by substituting, deleting or adding one or more nucleotides to the nucleotide sequence as shown in SEQ ID NO.1 and expresses protein with the same function. According to the invention, a coding gene of an atractylodes macrocephala terpene synthase gene (AmTPS) is cloned from the rhizome of the atractylodes macrocephala, and the synthase can be applied to a pathway prepared by taking farnesyl pyrophosphate (FPP) as a substrate. By utilizing the gene provided by the invention, the content of terpenoids in bighead atractylodes rhizome can be increased through a genetic engineering technology.

The present invention relates to a protein-ribonucleic acid complex comprising (I) at least one multi-functional protein or a set of at least two proteins and (II) a nucleic acid editor complex, a polynucleotide encoding the multi-functional protein (I) and its uses in the delivery of nucleic acids and gene editing tools. The present invention also relates to a nucleic acid delivery system, a plurality of polynucleotides, pharmaceutical compositions, a cell or a cell line, and kits. The present invention further relates to medical uses of the nucleic acid delivery system or the cell or cell line. Also provided are uses of a cell or a cell line in therapeutic and diagnostic applications, as well as in tissue engineering. Finally, the present invention further relates to an in vitro or in vivo method of editing a nucleic acid molecule in a target cell, and a method for nucleic acid delivery.

The invention provides paeonia suffruticosa-derived phosphatidyldiacylglycerol acyltransferase 1-2 or a coding gene and application thereof, and belongs to the technical field of functional proteins. The invention relates to phosphatidyldiacylglycerol acyltransferase 1-2 from paeonia ostii, which comprises phosphatidyldiacylglycerol acyltransferase 1-2 from paeonia ostii and / or phosphatidyldiacylglycerol acyltransferase 1-2 from paeonia suffruticosa. Experiments show that the Paeonia ostii-sourced phosphatidyldiacylglycerol acyltransferase 1-2 and / or Paeonia fulva-sourced phosphatidyldiacylglycerol acyltransferase 1-2 not only can synthesize linoleic acid and alpha-linolenic acid by using exogenous polyunsaturated fatty acid, but also does not affect accumulation of yeast total lipid. The peony-sourced phosphatidyldiacylglycerol acyltransferase 1-2 provided by the invention provides a new method for biosynthesis of triacylglycerol, and provides a new way for genetic breeding of oil crops.

The invention discloses a protein-based composite system for embedding linoleic acid based on a physical field as well as a preparation method and application of the protein-based composite system, and relates to the field of compositions of high-molecular compounds. According to the method, the protein compound is modified through DHPM or TSE, no organic solvent is used, heating is not needed, and the method is a novel method for obtaining the green and safe functional protein compound and is environmentally friendly and efficient. And meanwhile, a high-pressure emulsion homogenizing technology is adopted to prepare an emulsion loaded with linoleic acid, so that the stability of the linoleic acid is improved, the bioaccessibility of the linoleic acid is improved, and controllable slow release of the linoleic acid in the intestinal tract is realized. The prepared protein-based composite system based on physical field embedded linoleic acid has excellent solubility, thermal stability and oxidation resistance, can resist gastrointestinal tract temperature and is suitable for gastrointestinal tract absorption when people eat the protein-based composite system, and can be applied to edible nutritional factor delivery products.

The application discloses a method for preparing mesoporous zeolite under normal temperature and pressure and application thereof, and the preparation method comprises the following steps: (1) preparation of a functional protein biomodule: cationic polypeptide is loaded on yeast to obtain a biomodule aqueous solution; (2) deposition of silicon and aluminum: the pH of the biomodule solution obtained in the step (1) is adjusted to neutral, then a silicon source, an aluminum source and a titanium source are added into the solution, and the solution is incubated on a shaking table for 3 days; (3) calcination: the solid obtained in the step (2) is collected by centrifugation, washed with ethanol and water alternately for 3 times, and calcined at 500 DEG C for 4h under an air atmosphere to obtain a zeolite molecular sieve adsorption material. The application can prepare porous molecular sieve small balls by using a simple method, and can provide a brand-new thought for synthesizing molecular sieve materials.

The present invention relates to a rep plasmid comprising at least one long adeno-associated virus replication protein coding sequence encoding at least one long functional rep protein, at least one short adeno-associated virus replication protein coding sequence encoding at least one short functional rep protein, and at least one heterologous element, wherein following translation the at least one heterologous element results in a ratio of long adeno-associated virus rep protein to short adeno-associated virus rep protein of about 1:5 to about 1:5000.

The application belongs to the field of new application of functional proteins and mutants thereof, and discloses application of sugarcane ScCBS protein and key domain deletion mutants thereof. The nucleotide sequence of the sugarcane ScCBS protein is shown as SEQ ID NO:1, and the amino acid sequence is shown as SEQ ID NO:2. The key domain deletion mutants of the ScCBS protein are ScCBS△motif1, ScCBS△motif3, ScCBS△motif4 and ScCBS△motif9, which respectively lack 28, 50, 49 and 29 amino acids. The application proves that the ScCBS protein can interact with SCSMV P1 and inhibit the RNA silencing suppressor activity by means of yeast two-hybrid, two-molecular fluorescence complementary experiment and agrobacterium transient expression system; the four mutants all lose the interaction and inhibition activity. Therefore, the ScCBS protein and the key domain deletion mutants thereof can be used for cultivating excellent SCSMV-resistant materials, and have wide application prospects in sugarcane disease-resistant molecular breeding.

The invention belongs to the technical field of agricultural biology, and provides a soybean nodulation and nitrogen fixation related gene and application of an encoding protein thereof, the nucleotide sequence of the gene Gm02g072800 is shown as SEQ ID NO: 1, or a variant sequence which encodes the same functional protein and has homology greater than or equal to 90%; the amino acid sequence of the protein coded by the gene Gm02g072800 comprises a conservative AP2 structural domain, belongs to an ERF transcription factor family, is positioned in a cell nucleus, and has the functions of promoting soybean root nodule development and symbiotic nitrogen fixation. According to the invention, the specific expression of the gene in the soybean middle column and root nodule endodermis is determined, and a target gene is provided for cultivating soybean varieties with high nitrogen fixation efficiency.

The application provides a combined drug, a liposome, a biomimetic liposome, a preparation method of the combined drug, the liposome and the biomimetic liposome, and application of the combined drug, the liposome and the biomimetic liposome in preparation of an anti-breast cancer lung metastasis drug, and belongs to the technical field of biological medicines. The combined drug comprises panatinib and ginsenoside Rg3. The application further provides a liposome PNT-Rg3-Lipo for simultaneously loading the two, and a biomimetic liposome MM / PNT-Rg3-Lipo coated with a macrophage membrane. Experiments show that the combination of the two can synergistically regulate chemokines CXCL1 , CXCL2 and CXCL11 , and the effect is better than the sum of the single use. The MM / PNT-Rg3-Lipo has uniform particle size, retains integrin alpha 4 / beta 1 functional proteins, can target lung metastases, significantly reduces metastatic nodules, and has good biocompatibility. The application provides an effective new strategy for resisting breast cancer lung metastasis.

The invention relates to a helper adenovirus genome wherein expression of one or more of the adenoviral early E1 genes, E2 genes including DNA-binding protein (DBP), pre-terminal protein (pTP) and DNA polymerase, or production of functional proteins are regulated. The invention also relates to a process for delivery of the helper-dependent or gutless adenovirus vector genome and modulating replication of the helper virus DNA in the manufacturing cells, in which to allow a sufficient length of time before excision, cutting, or recombination to removal of the viral packaging signal DNA sequence from the helper adenovirus genome prior to induction of viral DNA replication, and expression of helper proteins that are required for adenovirus capsid particle formation and packaging, or encapsidation, of the helper-dependent or gutless adenovirus vector genomes, while preventing the packaging, or encapsidation, of the helper adenoviral genome into preform adenoviral capsids.

The invention discloses high-solubility and high-stability protein powder containing an acidic functional component. The protein powder comprises the following components in percentage by mass: 70-92% of soybean protein isolate, 1-3% of the acidic functional component and 4.52-16.5% of a composite dissolution-promoting dispersion-stabilizing component, the composite dissolution-promoting and dispersion-stabilizing component is prepared from the following components in percentage by mass: 3 to 10 percent of isomaltooligosacharide, 1.5 to 6 percent of fructo-oligosaccharide and 0.02 to 0.5 percent of stevioside; the invention further discloses a preparation method of the high-solubility and high-stability protein powder containing the acidic functional components. The invention further discloses an acidic functional protein beverage and a preparation method thereof. The brewing dissolution speed and the solution stability are remarkably improved through an oligosaccharide-sweetening agent compounding system, the dry mixing process is simple, complex modification treatment is not needed, the cost is lower than that of an enzymolysis or microcapsule technology, and the good industrialization advantage is achieved.

The invention relates to the field of plant genetic engineering, and discloses a corn sheath blight disease-resistant gene GRMZM2G057329 and application thereof, and the corn sheath blight disease-resistant gene GRMZM2G057329 comprises any one of the following sequences: a nucleotide sequence shown as SEQ ID No.1, a nucleotide sequence shown as SEQ ID No.2, a nucleotide sequence shown as SEQ ID No.3, a nucleotide sequence shown as SEQ ID No.4, a nucleotide sequence shown as SEQ ID No.5, a nucleotide sequence shown as SEQ ID No.6, a The nucleotide sequence is as shown in SEQ ID No.2; the amino acid sequence of the protein is shown as SEQ ID No.3; the nucleotide sequence has 90% or more of identity with the nucleotide sequence as shown in SEQ ID No. 1 or SEQ ID No. 2 and is used for coding protein with the same function as the nucleotide sequence as shown in SEQ ID No. 1 or SEQ ID No. 2. According to the present invention, the DNA fragment of the complete coding segment of the anti-disease gene GRMZM2G057329 separated and cloned from corn is adopted, and the GRMZM2G057329 gene expression quantity is increased so as to significantly enhance the sheath blight resistance of the corn genetic transformation plant;

The invention belongs to the field of microorganisms and medicine health care, and relates to an ackermania muciniphila strain and application thereof, the preservation number of the ackermania muciniphila strain is CGMCC (China General Microbiological Culture Collection Center) No.34370, and a 16SrDNA (Deoxyribose Nucleic Acid) sequence of the ackermania muciniphila strain has 93% similarity with a sequence shown as SEQ ID NO: 1. The new ackermania muciniphila strain (AKKBC300) is separated from a healthy human body and has good safety; good tolerance to low pH value and bile salt is shown in vitro, and it is indicated that the compound can reach the intestinal tract through the upper digestive tract and play a role; according to the present invention, genomics analysis results prove that the bacterial strain contains the gene Akkermansia BC00573 with high homology with the known probiotic functional protein Amuc1100, such that the molecular basis is provided;

The invention belongs to the field of biomedicine, and particularly relates to a detection method, an evaluation method and application of umbilical cord blood-derived natural killer cells. The detection method disclosed by the invention is used for carrying out related detection and data analysis on the natural killer cells from umbilical cord blood, is high in pertinence and can be used as a detection method for delivering the NK cells from a warehouse. According to the evaluation method, the relationship between the NK function related protein and the cell killing ability is determined, the killing ability of the NK cells is judged by detecting functional protein indexes CD3, NKG2A, CD56, CD16, NKP46 and CD107A of the natural killer cells and calculating NK scores of samples based on detection results, the sensitivity and specificity are high, and the method can be used for evaluating the killing ability of the NK cells.

The present disclosure relates to the field of protein detection technology, and discloses a protein detection method based on a combined nanopore structure, comprising: a surface of a sandwich film chip is processed to form a nano-through hole, and a “hole-cavity-hole” structure is prepared. The “hole-cavity-hole” structure is driven by the electric field of the functional protein to detect the protein. The present disclosure adopts the above-mentioned protein detection method based on a combined nanopore structure, which can effectively overcome the problem of protein detection reading length caused by the limitation of nanopore space. Meanwhile, in contrast to methods that embed functional protein complexes within a phospholipid bilayer, integrating functional proteins directly into solid nanopores combines the advantages of both biological and solid systems. This approach not only relaxes the stringent environmental demands placed on the sensor but also enhances its resolution and stability.

This invention discloses a method and application for efficient synthesis of human lactoferrin through multi-copy integration, relating to the fields of genetic engineering and bioengineering. The invention provides a method for synthesizing human lactoferrin, including the steps of fermenting and culturing a recombinant Aspergillus niger strain, followed by isolation and purification to obtain human lactoferrin; the construction method of the recombinant Aspergillus niger strain includes the step of integrating the hLF gene into a gene locus in the host strain genome to construct the recombinant Aspergillus niger strain. This invention constructs a recombinant Aspergillus niger strain capable of recombinantly expressing human lactoferrin, initiating protein expression through a strongly inducible promoter, fusing endogenous high-secretion proteins, and optimizing the multi-copy integration method to integrate the lactoferrin expression cassette, achieving high-level synthesis of human lactoferrin, laying the foundation for the industrial production of lactoferrin or high-density fermentation of other food functional proteins using Aspergillus niger.