29 results about "Functional protein" patented technology

The application belongs to the field of new application of functional proteins and mutants thereof, and discloses application of sugarcane ScCBS protein and key domain deletion mutants thereof. The nucleotide sequence of the sugarcane ScCBS protein is shown as SEQ ID NO:1, and the amino acid sequence is shown as SEQ ID NO:2. The key domain deletion mutants of the ScCBS protein are ScCBS△motif1, ScCBS△motif3, ScCBS△motif4 and ScCBS△motif9, which respectively lack 28, 50, 49 and 29 amino acids. The application proves that the ScCBS protein can interact with SCSMV P1 and inhibit the RNA silencing suppressor activity by means of yeast two-hybrid, two-molecular fluorescence complementary experiment and agrobacterium transient expression system; the four mutants all lose the interaction and inhibition activity. Therefore, the ScCBS protein and the key domain deletion mutants thereof can be used for cultivating excellent SCSMV-resistant materials, and have wide application prospects in sugarcane disease-resistant molecular breeding.

The application provides a combined drug, a liposome, a biomimetic liposome, a preparation method of the combined drug, the liposome and the biomimetic liposome, and application of the combined drug, the liposome and the biomimetic liposome in preparation of an anti-breast cancer lung metastasis drug, and belongs to the technical field of biological medicines. The combined drug comprises panatinib and ginsenoside Rg3. The application further provides a liposome PNT-Rg3-Lipo for simultaneously loading the two, and a biomimetic liposome MM / PNT-Rg3-Lipo coated with a macrophage membrane. Experiments show that the combination of the two can synergistically regulate chemokines CXCL1 , CXCL2 and CXCL11 , and the effect is better than the sum of the single use. The MM / PNT-Rg3-Lipo has uniform particle size, retains integrin alpha 4 / beta 1 functional proteins, can target lung metastases, significantly reduces metastatic nodules, and has good biocompatibility. The application provides an effective new strategy for resisting breast cancer lung metastasis.

The present disclosure relates to the field of protein detection technology, and discloses a protein detection method based on a combined nanopore structure, comprising: a surface of a sandwich film chip is processed to form a nano-through hole, and a “hole-cavity-hole” structure is prepared. The “hole-cavity-hole” structure is driven by the electric field of the functional protein to detect the protein. The present disclosure adopts the above-mentioned protein detection method based on a combined nanopore structure, which can effectively overcome the problem of protein detection reading length caused by the limitation of nanopore space. Meanwhile, in contrast to methods that embed functional protein complexes within a phospholipid bilayer, integrating functional proteins directly into solid nanopores combines the advantages of both biological and solid systems. This approach not only relaxes the stringent environmental demands placed on the sensor but also enhances its resolution and stability.

The present disclosure relates to methods, devices and systems for exposing microorganisms or compositions comprising functional proteins to an oscillating electric field substantially free of a magnetic component in order to improve stress responses, survival and metabolite or protein production. In embodiments, a controller drives one or more insulated emitters with a true AC signal or pulsed DC signal, in stepped, swept or shuffled patterns of an electric field oscillating at one or more frequencies in the range 210 Hz to 99 kHz. Exposure to the field provides surmounting energy that enhances protein breathing, catalysis, and fundamental functions of multiprotein complexes including transcription factors and ribosomes, thereby improving stress resistance of microorganisms, their viability during fermentation, drying, storage, transport, and / or increasing production of functional or therapeutic proteins, metabolites or microbial lysates. The technology is applicable to manufacture of probiotics, therapeutics, metabolites, food, alcohol, feed and bioenergy, cosmetics, nutraceuticals and biopharmaceuticals..

The application discloses a method for collecting and purifying mouthpart secretion of a predatory natural enemy Orius minor, and belongs to the technical field of biology. The method comprises the following steps: constructing a double-layer membrane sandwich artificial feeding device, encapsulating a nutrient solution in a sandwich space between a first membrane layer and a second membrane layer, and using the feeding drive of the Orius minor to make the Orius minor actively pierce the membrane layer and feed the nutrient solution, so that the mouthpart secretion is released into the nutrient solution in the natural feeding process; after the nutrient solution containing the secretion is collected, ultrafiltration centrifugation technology is used for concentration and purification, and a high-purity mouthpart secretion protein sample is obtained. The natural feeding state of the Orius minor is simulated, the collected secretion can more truly reflect the functional protein released by the Orius minor in the predation process, meanwhile, the integrated design of collection and purification effectively removes small molecular impurities in the sample, and the sample can be directly used for high-precision analysis such as proteomics.

The application belongs to the field of biological detection, and particularly relates to a specific monoclonal antibody of an AKK bacterial efficacy protein Amuc_1100, a kit, a detection method and application thereof. The antibody pair is 3E10 and 2F7 monoclonal antibodies, the heavy chain and light chain variable region amino acid sequences of 3E10 are SEQ ID NO. 2 and SEQ ID NO. 4, and the heavy chain and light chain variable region amino acid sequences of 2F7 are SEQ ID NO. 6 and SEQ ID NO. 8. The antibody pair has high specificity and high affinity, a colloidal gold rapid detection kit and a double antibody sandwich ELISA quantitative detection kit based on the antibody pair realize rapid screening and accurate quantification of the Amuc_1100 protein, have good linearity, high precision and excellent accuracy, and can be used for detection of the protein in mucoid Akkermansia and quality control and activity evaluation of related probiotic preparations, thereby providing key technical support for related basic research and industrial application.

The application provides a curcumin composition-FGF21 functional protein co-assembly microparticle applied to liver diseases and a preparation method thereof, and comprises the following steps: extracting turmeric by dynamic warm soaking, obtaining turmeric filtrate after centrifugation, concentration and microfiltration; preparing high-purity curcumin composition through PS-NVP specific purification and 80% ethanol gradient elution; and preparing Curs-FGF21 co-assembly microparticles through oil-water phase assembly and centrifugation by taking a polymer as a carrier, compounding the curcumin composition with FGF21 protein. The microparticle can improve drug stability and bioavailability, realizes long-acting slow release, can regulate liver cell lipid metabolism, reduce liver lipid accumulation and inflammation, significantly improves liver damage and liver fibrosis, and has outstanding liver protection and repair effects. The process is stable and controllable, has outstanding innovation, and provides a brand-new candidate preparation research and development idea and technical scheme for the prevention and treatment of metabolic liver diseases.

A method of obtaining cell derived vesicles comprising an active wild-type p53 is disclosed. The method comprising: (i) isolating cell derived vesicles from a biological sample comprising cells; and (ii) treating the cell derived vesicles with a DNA damaging agent, or the method comprising: (i) treating cells with a DNA damaging agent; and (ii) isolating cell derived vesicles from a biological sample comprising the cells. A proteinaceous preparation comprising cell derived vesicles and a pharmaceutical composition comprising the proteinaceous preparation are also disclosed. Methods of treating a disease, disorder or condition associated with a mutant or a nonfunctional p53 protein and methods of inducing apoptosis of a target cell comprising a mutant or a nonfunctional p53 protein are also disclosed.

Isolated or purified antisense oligomers, combinations, and cocktails for modifying premRNA splicing or mRNA translation in the SOST gene transcript or a portion thereof to induce downregulation of functional SOST protein production. Isolated or purified antisense oligomers are provided, as needed, for inducing the production of proteins with retained or partially retained introns, truncated proteins, proteins lacking functional regions, or a reduction in the total amount of protein produced.

The utility model relates to the technical field of feed production discloses a functional protein feed production device of piglet, including fermentation tank, the inner surface fixed mounting of fermentation tank has stirring fermentation mechanism, the outer surface of fermentation tank is connected with self -adaptation aeration mechanism, the stirring fermentation mechanism includes stirring motor, the bottom of stirring motor is fixedly connected with the top of fermentation tank, the output end of stirring motor is fixedly installed with stirring shaft through speed reducer, the bottom end of stirring shaft penetrates fermentation tank and extends to the inside of fermentation tank. The functional protein feed production device of piglet, through stirring motor drives stirring shaft to rotate, and the hydraulic cylinder drives the hydraulic rod to rotate through stirring shaft, and the hydraulic rod drives the equipment box to rotate, and the equipment box moves up and down through the hydraulic cylinder drives the equipment box through the hydraulic rod, and the rotary motor drives the stirring board to rotate through the rotating shaft in the equipment box, and through above a series of operation, makes the stirring board to be able to extend into the fermentation tank different depth.

This invention discloses the application of a UBC9 overexpression reagent in the preparation of drugs for preventing hypothermia caused by acute cold exposure, belonging to the field of biomedical technology. In vitro and in vivo experiments confirmed that acute cold exposure can induce a significant upregulation of UBC9 expression in skeletal muscle. Further, a skeletal muscle-specific UBC9 overexpression mouse model was constructed, revealing that UBC9 overexpression significantly enhances the core body temperature maintenance capacity of mice under acute cold exposure, upregulates the expression of the key thermogenic protein UCP3 and mitochondrial functional proteins TOMM20 and COX4, alleviates mitochondrial structural damage, and reduces mitochondrial oxidative stress levels. This invention reveals for the first time that UBC9 is a key target for the regulation of skeletal muscle thermogenicity under acute cold exposure. Enhancing UBC9 expression can effectively prevent or improve skeletal muscle thermogenic dysfunction caused by cold environments, providing a novel intervention strategy for developing drugs to prevent and treat cold injury.

The application discloses application of ACTRT1 as a target to detection and treatment of headless sperm. In the application, ACTRT1 gene mutation causes deletion of ACTRT1 functional protein, may affect connection of sperm head and tail, and finally induces headless sperm.

This invention discloses an umbilical cord mesenchymal stem cell membrane for treating hair loss, its preparation method, and its application, belonging to the field of biomedical technology. The preparation method of the umbilical cord mesenchymal stem cell membrane includes the following steps: obtaining human umbilical cord mesenchymal stem cells, digesting, centrifuging, washing, and resuspending them to obtain a resuspended cell solution; subjecting the resuspended cell solution to repeated freeze-thaw cycles to break the cells, obtaining a cell lysate; and subjecting the cell lysate to differential centrifugation to obtain the umbilical cord mesenchymal stem cell membrane. This umbilical cord mesenchymal stem cell membrane retains the natural cell membrane structure and functional proteins, has a negatively charged surface, and exhibits good dispersibility and stability. It can improve the survival rate of dermal papilla cells without introducing live cells and effectively regulate the skin microecology and metabolite balance, demonstrating high safety, good biocompatibility, and excellent hair growth promotion effects.

The present application relates to the technical field of feed, in particular to a functional protein feed produced by using enzymatic decoupling fermentation process to treat the meal and a preparation method thereof. The method mixes double-low rapeseed meal, de-phenol cottonseed protein and peanut meal according to a specific ratio, and sieves to obtain coarse phase and fine phase. By adding slow-release type embedded acid protease wet capsules (type B) to the fine phase, adding fast-release type embedded acid protease wet capsules (type A) to the coarse phase, and adopting the process of swelling the fine phase first and then carrying out anaerobic fermentation with the coarse phase, the time sequence and space control release of the protease in the fermentation process are realized. The technology significantly improves the enzymatic hydrolysis efficiency of protein and the degradation effect of anti-nutritional factors, and the final product has high nutritional value and can effectively improve the growth performance of weaned piglets.

The application belongs to the technical field of protein purification, and discloses a protein purification method based on condensate, which comprises the following steps: firstly, constructing a target expression vector; secondly, expressing a fusion protein with an LplA label in E. coli; thirdly, ultrasonically crushing the E. coli, centrifuging the crushing liquid, and taking supernatant; fourthly, performing first temperature change, heating the supernatant, centrifuging and collecting the fusion protein after the fusion protein forms condensate, discarding the supernatant, and taking the precipitate; and fifthly, performing second temperature change, redissolving the precipitate obtained in the fourth step in a buffer at low temperature, removing insoluble proteins by centrifugation at low temperature, and taking the supernatant as the fusion protein with the LplA label after purification. The application connects the temperature-responsive purification label LplA which can form condensate to the target protein, and only needs to perform two temperature change treatments to quickly obtain the target protein with high purity, without the need of complex chromatography equipment and expensive fillers, and the application is suitable for large-scale production and purification of functional proteins.

This invention discloses a complex functional protein peptide derived from sea cucumber, its formulation, and its applications, belonging to the field of bioactive peptide technology. The amino acid sequence of the complex functional protein peptide derived from sea cucumber in this invention is at least one of FFGG, FGAF, and FDGF. Using sea cucumber blanching solution as raw material, this invention employs methods such as protease hydrolysis, LC-MS / MS, and bioinformatics to separate and purify three bioactive peptides with antioxidant and anti-allergic effects. Based on the identified peptide sequences, chemical synthesis is performed, and the antioxidant and anti-allergic effects of the synthesized single peptides and complex peptides are measured. These bioactive peptides all exhibit high DPPH scavenging rates, ABTS free radical scavenging rates, and hyaluronic acid inhibition rates. Therefore, these bioactive peptides have significant application prospects in the development of products with antioxidant and anti-allergic effects.

The application discloses a functional protein screening method based on a large language model, and belongs to the technical field of protein screening, and specifically comprises the following steps: obtaining a protein three-dimensional structure and function literature, converting the protein three-dimensional structure and function literature into a multi-modal feature vector by using an encoder, and constructing a field-specific vector database; after receiving a screening instruction, associated feature data matched in semantics is called by a vector retrieval algorithm; subsequently, the data is input into a large language model to perform biological mechanism reasoning, and an initial candidate protein sequence containing predicted activity is output; further, a molecular docking program is used to calculate physical binding energy, a deviation coefficient of the initial candidate protein sequence and the predicted activity is generated, and the deviation coefficient is used as a penalty term to construct a dynamic correction function to update a scoring weight; finally, a secondary sorting is performed on a candidate set based on the updated weight, and a sequence ranked first is output as a target protein; the method effectively combines AI reasoning and physical verification, and improves screening accuracy.

This invention discloses a method and apparatus for solute removal in hemodialysis with precise membrane pore size control, belonging to the field of blood purification technology. The method involves introducing blood and dialysate into both sides of a dynamically controlled bilayer heterostructure dialysis membrane, collecting characteristic signals of metabolic solutes and plasma functional proteins in the blood, inputting these signals into an adaptive pore size decision model, and outputting target pore size and electric field strength parameters. A low-frequency alternating electric field drives reversible conformational changes in stimulus-responsive polymer chains within the membrane, achieving precise control of the membrane channel's effective sieving pore size, locking the pore size within a threshold range where small molecule solutes permeate while plasma functional proteins are completely retained. Combining axial gradient channels and radial charge sieving mechanisms within the membrane achieves graded and targeted solute removal. A piezoelectrically driven periodic micro-pulsating flow field removes the fouling layer from the membrane surface, improving mass transfer stability. This invention automates and precisely controls the dialysis process, improving solute removal efficiency and plasma protein protection.

Monocot plants heterozygous for centromeric histone 3 (CenH3) and optionally expressing gene editing constructs, for use in inducing haploids of a monocot target plant and optionally pass-through gene editing are provided. The monocot haploid inducer plants are typically composed of diploid plant cells having only one allele encoding a functional CENH3 protein. The diploid plant cells can also include, for example, one CenH3 allele encoding non-functional CENH3 protein. In some embodiments, the allele encoding non-functional CENH3 protein is a frameshift mutation, protein null allele, an RNA null allele, or a combination thereof. The monocot haploid inducer plant can also include gene editing machinery, such as a site-directed nuclease and optionally a guide RNA stably expressed by cells of the monocot plant. Methods of inducing formation of a target haploid monocot plant while optionally simultaneously modifying the target monocot plant's genome are also provided.

The present application belongs to the field of biotechnology, and specifically relates to a method for expressing human serum albumin by using Nicotiana sylvestris NS suspension cell line, which comprises the following steps: establishing a stably cultured Nicotiana sylvestris suspension cell system, and producing by amplification, and the obtained cell biomass can reach up to 500 g / L. Then, taking human serum albumin as the research object, transformation screening is carried out in the Nicotiana sylvestris NS suspension cell system to realize high expression of HSA. The present application removes the complex tissue regeneration step, and through plant codon optimization and subcellular targeting strategy design, aims to overcome the problem of low expression of exogenous genes in plants, so as to realize efficient enrichment of target proteins in specific organelles (such as vacuole / endoplasmic reticulum, etc.). The technical scheme of the present application can be popularized and applied to the expression and screening of other types of functional proteins, and provides important technical reference and practical support for the application of plant suspension cell lines in the field of recombinant protein expression.

The invention relates generally to a method of treatment for a human genetic disease, such as diseases characterized by unbalanced nucleotide pools, e.g., mitochondrial DNA depletion syndromes, and more specifically, thymidine kinase 2 (TK2) deficiency, using gene therapy. The gene therapy may involve administration of one or more constructs, such as a viral vector, containing a nucleic acid encoding a functional protein. The functional protein may correspond to a nuclear gene. For treatment of TK2 deficiency, the gene therapy may involve administration of one or more constructs, such as a viral vector, containing a nucleic acid encoding a functional TK2 enzyme. The treatment may also involve the administration of pharmacological therapy in conjunction with the gene therapy. The treatment protocols of the disclosure, such as those involving gene therapy alone or in combination with pharmacological therapy, can be used to treat, prevent, and / or cure various other disorders of unbalanced nucleoside pools, especially those found in mitochondrial DNA depletion syndrome.

This invention discloses a method for preparing and applying a low-chitin enzymatic hydrolysed cricket feed based on Trichoderma and copepod autolysins, belonging to the field of aquaculture technology. This invention employs a combined enzymatic hydrolysis method using Trichoderma activation and copepod autolysins, which not only significantly reduces the chitin content in the cricket feed (to below 1%), but also optimizes the nutritional composition of the feed by introducing marine functional proteins from copepods. This not only replaces fishmeal in feed but also contributes to the growth and health of aquatic animals.

This invention discloses a method for preparing the BjIM1 protein and gene from amphioxus and its application in lupus erythematosus, belonging to the field of biopharmaceutical technology. The process includes: S1. Material acquisition: collecting amphioxus from Qingdao; S2. Screening and preparation of BjIM1 protein: S2.1, screening for differentially expressed proteins under LPS stimulation; S2.2, screening for differentially expressed proteins with significantly upregulated LPS and low homology to known functional proteins, exhibiting anti-inflammatory activity by inhibiting pro-inflammatory factor expression and regulating immune tolerance; S3. Cloning and preparation of the BjIM1 gene: S3.1, extracting total RNA from amphioxus tissue; S3.2, designing specific primers based on the amino acid sequence of the BjIM1 protein; S3.3, amplifying the gene sequence using RACE technology, and assembling to obtain the full-length BjIM1 gene sequence containing a complete ORF; S3.4, verifying the result by PCR amplification and sequencing, obtaining the amphioxus BjIM1 gene encoding the BjIM1 protein. This invention possesses the technical advantages of definite anti-inflammatory effect, precise immune regulation, high biosafety, no risk of drug resistance, and stable and reproducible process.

This application provides an expression system and a method of using the same, and its applications. The expression system comprises a polynucleotide that enhances the production of mogrosides, the polynucleotide comprising at least one of several protein coding nucleotides that enhance the production of mogrosides, the protein comprising an enzyme and / or functional protein in the mogroside synthesis pathway, and being at least one selected from SQE, EPH, DREB1c, CDS, CYP87D18, UGT720, UGT94, and FTO.

The application belongs to the technical field of soybean functional protein structure application, and more particularly relates to a high-function-factor-loading-capacity amyloid fiber as well as a preparation method and application thereof. The application utilizes a natural deep eutectic solvent to carry out deamidation treatment on soybean protein, which can not only promote the soybean protein to form amyloid fibers more efficiently, but also can significantly improve the loading capacity of the formed amyloid fibers on functional factors (such as tannic acid), thereby providing a new solution for developing high-performance protein-based delivery carriers.

The application discloses a method for improving expression of exogenous glycoprotein by yeast, comprising the following steps: without changing endogenous genes of a yeast host cell and without introducing exogenous sugar chain processing enzymes, an exogenous N-acetylglucosamine transferase I (GnT1) gene from different species is integrated into a genome of a Pichia pastoris host bacterium for expressing glycoprotein, and a Pichia pastoris engineering bacterium for expressing the exogenous GnT1 is constructed, so that the expression amount of the exogenous glycoprotein in the Pichia pastoris engineering bacterium is significantly improved, the proportion of high mannose type N-glycan is reduced, and the proportion of complex type N-glycan is increased. The method is suitable for biosynthesis of polysaccharide glycosylation protein, high isoelectric point protein and complex folding protein, and has good popularization and application prospect in the fields of biological pharmacy, functional protein, nutritional and healthy raw material and industrial biological manufacturing.

This invention discloses a plant nanogene delivery formulation, a method for nanogene delivery and functional protein expression in plants, and its applications, relating to the field of biotechnology. The nanogene delivery and functional protein expression method involves using a nanogene delivery formulation to achieve efficient gene delivery to plant receptors and expression of functional proteins under ultrasound assistance, and increasing the secretion level of functional proteins in plant cells through secretory peptide sequences. The plant nanogene delivery formulation comprises a modified mesoporous silica (MSN) carrier and nucleic acid molecules loaded on the MSN carrier, wherein the CMSN uses the MSN carrier as a backbone, with polyethyleneimine and cell-penetrating peptides bonded to its surface. The functional proteins produced by this invention possess complete spatial structures and exhibit effects such as wound healing, skin regeneration, and cosmetic skincare. This invention is beneficial for producing highly active functional proteins with correctly folded spatial structures.

The application provides a kind of synapse-like nerve prosthesis adhesive, its preparation method, application and injection needle.The adhesive includes functional protein, reducing sugar, lithium salt and moisture, forms hydrogel;Functional protein includes natural biological protein and small molecule polyphenol, oxidative coupling forms irreversible covalent bond crosslinking network;Hydrogen bond forms rearrangeable intermolecular interaction crosslinking network;Lithium ion and the quinone group formed by oxidation constitute reversible redox couple, catechol and lithium ion form reversible coordination bond.The adhesive provided by the application introduces triple crosslinking mechanism of covalent bond, freely rearrangeable hydrogen bond and reversible ionic coordination bond, so that hydrogel has high mechanical strength, excellent self-repairing ability and injectability, solves the problem of limited function of existing single crosslinking mode hydrogel, electrical signal transmission mechanism simulates natural synapse, avoids gas generation, electrode oxidation and tissue damage problem, and can realize rapid nerve tissue repair and significant recovery of motor function.

The application discloses a plant-based functional protein composition with immunoregulation and application thereof, and belongs to the technical field of functional food. The composition takes plant-derived proteins and protein peptides as cores, and contains peanut peptides 40-60%, seaweed proteins 5-10%, and soybean peptides 1-5% in terms of mass percentage. Optionally, the composition can further contain pea proteins 1-3%, quinoa proteins 0.1-2%, and 3-20% of food-grade auxiliary materials. The application can significantly regulate the immune activity of macrophages by scientifically compounding various plant-derived active ingredients, and the synergistic effect of the components. The composition has wide raw material sources, high safety, and balanced nutrition, and can be widely applied to the preparation of immunoregulation food or health food, and is suitable for various people who need to enhance immunity, so that the application has wide market application prospects.