353 results about "Functional protein" patented technology

The invention relates to the technical field of protein purification, in particular to a biological method for purifying fusion protein through two different affinity tags. Two affinity purification tags and a dissolution promoting tag are perfectly combined, so that soluble expression of recombinant protein is ensured, high-purity target protein can be obtained through a two-step mild and efficient affinity purification process, the function and activity of the protein are ensured, a complex'capture-medium purification-fine purification 'multi-step purification process is avoided, and the method is suitable for industrial production. The purification process is shortened, the purification time is shortened, the purification efficiency is improved, and the method can be used for production and purification of functional proteins.

The invention provides a plug-and-play Mi3 protein nanocage fusion protein with a VNP6 tag and a preparation method and application of the plug-and-play Mi3 protein nanocage fusion protein, the fusion protein comprises the VNP6 tag, a SpyCatch003 component, a Mi3 protein nanocage and a functional protein sequence, and the VNP6 tag is located at the N end of the fusion protein. The VNP6 peptide sequence or the variant thereof is utilized to induce a vesicle or vesicle-like structure beneficial to protein folding and stabilization in escherichia coli, so that the correct folding rate and activity of recombinant protein are improved while the overall intracellular molecular crowding effect is relieved. The core idea of the invention lies in that the internal structure of cells is regulated at low temperature, and the internal microenvironment of an escherichia coli expression system is optimized, so that protein molecules under high expression load can obtain more reasonable spatial distribution, and the problems of misfolding and aggregation caused by molecular crowding are reduced.

A method for functional antimicrobial peptide design is provided. The method includes running pretrained protein large language models as a generator and enhancing a sample candidate. The enhancing a sample candidate includes performing an automatic pipeline based on machine learning methods and a plurality of bioinformatics methods and is configured to perform protein inverse folding and computational protein sequence designing. The computational protein sequence designing includes running a pretrained deep learning-based protein structure model, an autoregressive pretrained protein sequence model, and a deep learning-based alignment model. The pretrained deep learning-based protein structure model is configured to learn three-dimensional structures of the proteins and output latent structure embeddings in a high dimensional space. The autoregressive pretrained protein sequence model includes a protein language model to automatically generate protein sequences and is trained to predict next amino acid in a protein sequence based on a preceding sequence(s) of amino acids.

The invention provides a fusion protein for promoting proliferation of hair follicle stem cells as well as a preparation method and application of the fusion protein. The fusion protein is formed by fusion expression of a plurality of collagen structural domains capable of being combined with cell integrin and / or specific structural domains from certain functional proteins screened from collagen XVII and III type collagen. Nucleic acid molecules for coding the fusion protein are subjected to codon optimization and then recombined and stably expressed in escherichia coli to obtain the fusion protein. The fusion protein has excellent performance of promoting proliferation and migration of human hair follicle stem cells and human fibroblasts, can be used for repairing skin and hair follicle tissues and improving resistance of skin and hair follicles, and is suitable for popularization and application in the fields of beauty and skin care, medical equipment, tissue engineering and the like.

The invention relates to a gene GL8 for controlling grain shape, appearance quality or / and yield of rice. The gene has (a) a nucleotide sequence as shown in SEQ ID No: 1; and (b) a nucleotide sequence which is generated by adding, substituting or deleting one or more basic groups from the nucleotide sequence as shown in SEQ ID No: 1 and is used for coding a functional protein for controlling the grain shape, the appearance quality and the yield of rice. The invention belongs to the technical field of gene engineering. By overexpressing the gene, the grain shape, appearance quality and yield of rice can be regulated and controlled.

The invention provides a BoNIA2b gene, which has a nucleotide sequence as shown in SEQ ID No.1, or a nucleotide sequence which is obtained by substituting, deleting and / or adding one or more nucleotides to the nucleotide sequence as shown in SEQ ID No.1 and expresses proteins with the same function, wherein the nucleotide sequence is obtained by substituting, deleting and / or adding one or more nucleotides to the nucleotide sequence as shown in SEQ ID No.1; the invention also provides an application thereof. Compared with the prior art, the broccoli BoNIA2b gene has the following advantages that functional verification is carried out on the broccoli BoNIA2b gene, it is found that overexpression of the BoNIA2b gene can promote increase of the content of glucoraphanin and sulforaphane in the hairy roots of broccoli, and it is found through further research that the content of glucoraphanin and sulforaphane in the hairy roots of broccoli can be increased; the overexpressed BoNIA2b gene is mainly used for regulating and controlling the expression level of some synthetic genes of glucoraphanin and sulforaphanin of the hairy roots of broccoli so as to regulate and control the synthesis of glucoraphanin and sulforaphanin. Finally, the BoNIA2b gene is proved to positively regulate the synthesis reaction of glucoraphanin and sulforaphane of broccoli.

The invention discloses a sea cucumber flower self-assembled polypeptide-selenium nano-carrier with antioxidant activity and a preparation method and application thereof, belongs to the technical field of functional protein peptides, and particularly relates to a preparation method of the sea cucumber flower self-assembled polypeptide-selenium nano-carrier with antioxidant activity, which comprises the following steps: heating a sea cucumber flower protein solution; the preparation method comprises the following steps: mixing sea cucumber with simulated gastric juice, reacting for 30-150 minutes, then adding simulated intestinal juice, reacting for 20-120 minutes, carrying out enzyme deactivation treatment, centrifuging, taking supernate, and freeze-drying to obtain sea cucumber flower self-assembled polypeptide; and mixing the sea cucumber flower self-assembled polypeptide with deionized water to prepare a sea cucumber flower self-assembled polypeptide solution, mixing the sea cucumber flower self-assembled polypeptide solution with a sodium selenite solution and a phosphate buffer solution, reacting for 20-60 minutes, washing with an ethanol solution, centrifugally collecting precipitate, and freeze-drying to obtain the sea cucumber flower self-assembled polypeptide-selenium nano-carrier. The sea cucumber flower self-assembled polypeptide-selenium nano-carrier prepared by the invention has good antioxidant activity and potential of effectively delivering selenium to an organism.

The invention relates to an industrial method, in particular to a method for preparing a universal functional protein nano-film on the surface of a base material, which comprises the following steps: (1) mixing a protein solution, an optional functional monomer and a photoinitiator solution to prepare a mixed solution; (2) soaking a base material in the mixed solution; and (3) irradiating with ultraviolet rays to obtain the protein nano-film on the surface of the base material. According to the universal method for continuously preparing the functional protein nano-film on the surface of the base material, rapid self-assembly aggregation and monomer polymerization of the protein can be carried out at the same time under ultraviolet induction, and the protein-functional polymer two-dimensional nano-film prepared on the surface of the base material is compact, low in roughness, high in transparency and good in application prospect. The biocompatibility is good.

The invention discloses a recombinant protein and application thereof in preparation of sodium alginate fibers. The recombinant protein is obtained by fusion expression of a functional protein and at least one alginic acid binding domain protein. The amino acid sequence of the alginic acid binding domain protein is as shown in at least one of SEQ ID No. 1, SEQ ID No. 2 and SEQ ID No. 3. The recombinant spider silk protein with the alginic acid CBM region can exert the material science characteristics in a sodium alginate-calcium ion system, the mechanical property of alginic acid fiber is greatly enhanced, and meanwhile, the biological activity of protein can be provided for an alginate fiber system.

Embodiments disclosed herein provide methods, including computer-implemented methods, for designing guide sequence which may be incorporated into custom, large scale guide sequence libraries. The methods require only a list of target genes as input and utilize on target and off target scores to generate an optimal set of guide sequences for a set of target genes. In certain embodiments, the methods may also utilize multi-tissue RNA-sequencing data and / or protein annotation to design targets to genes that are highly expressed and / or contain a functional protein domain. The invention further comprises guide libraries, cells comprising said guide libraries. Computer-implemented embodiments further improve computer system function by reducing excessive user wait time through the use of data structures that reduce search from linear to logarithmic time.

A construction and use of an environmentally adaptive co-regulated mRNA nanodelivery system. By incorporating EACR molecules into mRNA nanoparticles, the microenvironment is remodeled to be suitable for robust and sustained mRNA expression, while tissue damage associated with the self-immunogenicity of mRNA drugs is avoided. The nanodelivery system is compatible with ionizable lipid nanoparticles, cationic liposomes, cationic nanoemulsions, and polymeric nanoparticles. The EACR molecules include: anti-inflammatory drugs, tyrosine kinases / adaptors, JAK / STAT and MAPK pathway inhibitors, nutrients and metabolites, membrane transporters / ion channels, phosphodiesterase and cellular stress inhibitors, and natural viral proteins. The therapeutic mRNAs may encode tumor, viral, or bacterial antigens, immunomodulatory factors, therapeutic antibodies, or functional proteins / enzymes, and can play a role in the fields of regenerative medicine, protein supplementation / replacement therapy, targeted gene editing, and immunotherapy.

The invention discloses a functional protein screening method based on a large language model, and belongs to the technical field of protein screening, and the method specifically comprises the following steps: obtaining a protein three-dimensional structure and functional literature, converting the protein three-dimensional structure and functional literature into a multi-modal feature vector by using an encoder, and constructing a domain-specific vector database; after a screening instruction is received, related feature data of semantic matching is called through a vector retrieval algorithm; then, inputting the data into a large language model to execute biological mechanism reasoning, and outputting an initial candidate protein sequence containing predicted activity; further, physical binding energy is calculated by using a molecular docking program, a deviation coefficient between the physical binding energy and the predicted activity is generated, and the coefficient is used as a penalty term to construct a dynamic correction function to update the scoring weight; and finally, performing secondary sorting on the candidate set based on the updated weight, and outputting the sequence sorted for the first time as a target protein. According to the method, AI reasoning and physical verification are effectively combined, and the screening accuracy is improved.

The invention provides a VvYUC10 gene, the nucleotide sequence of the VvYUC10 gene is as shown in SEQ ID No.1, or the nucleotide sequence which is obtained by substituting, deleting and / or adding one or more nucleotides to the nucleotide sequence as shown in SEQ ID No.1 and expresses proteins with the same function; the invention also provides an application thereof. The grape VvYUC10 gene has the advantages that the function of the grape VvYUC10 gene is verified, it is found that the content of auxin in calluses of overexpressed VvYUC10 arabidopsis thaliana and grapes under salt stress is increased, the oxidation resistance is enhanced, and it is further found that the expression quantities of the VvRD22 gene, the VvDREB2A gene, the VvRD29A gene, the VvP5CS gene, the VvCAT1 gene and the VvSOD gene are increased; finally, it shows that the VvYUC10 gene positively regulates and controls the auxin signal channel to positively resist external adverse environmental factor salinization, and a theoretical reference basis is provided for grape salt-tolerant gene mining and breeding.

The invention discloses an application of a rape anthocyanin reductase BnaBAN gene or a protein coded by the BnaBAN gene in regulating and controlling the oil content of rape and the composition of unsaturated fatty acid. The nucleotide sequence of the BnaBAN gene is as shown in SEQ ID NO.1, or the sequence as shown in SEQ ID NO.1 is subjected to substitution and / or deletion and / or addition of one or more nucleotides and expresses a homologous gene of the same functional protein. An agrobacterium tumefaciens-mediated genetic transformation method is used for editing the BnaBAN gene, and it is found that BnaBAN mutation increases the oil content of rape seeds and affects fatty acid composition. The method disclosed by the invention has very important application prospects in high-oil-content breeding and oil quality improvement of the oilseed rape.

The invention discloses a scorpion-derived polypeptide and application thereof, and belongs to the technical field of functional proteins. The amino acid sequence of the polypeptide derived from the Buthus martensii Karsch is as shown in SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4 or SEQ ID NO. 5. The polypeptide derived from the Buthus martensii Karsch is a polypeptide derived from the Buthus martensii Karsch. The invention also provides an application of the buthus martensii-sourced polypeptide with the amino acid sequence shown as SEQ ID NO. 1 in preparation of a blood coagulation promoting drug and an application of the buthus martensii-sourced polypeptide with the amino acid sequence shown as SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4 or SEQ ID NO. 5 in preparation of a blood coagulation promoting drug. The buthus martensii-sourced polypeptide provided by the invention has a relatively good application value in the aspect of research and development of antithrombotic drugs or coagulant drugs.

The invention relates to the technical field of functional protein extraction, in particular to a mulberry leaf protein extraction method and application thereof. The extraction method comprises the following steps: dissolving mulberry leaf powder in water, and adjusting the pH value to be greater than or equal to 8 to obtain a mulberry leaf powder aqueous solution; carrying out digestion after ultrasonic crushing, and centrifuging to take supernatant as a crude extract; and adjusting the pH value of the crude extract to be less than or equal to 6, and centrifuging to take the precipitate. The method for extracting the mulberry leaf protein is provided by combining an alkali dissolution method and an ultrasonication method, the extraction rate of the mulberry leaf protein and the oxidation resistance of the extracted mulberry leaf protein can be effectively improved by combining the alkali dissolution method and the ultrasonication method, and the method has important application value for functional development of the mulberry leaf protein.

The invention discloses an application of CLASP2 protein in preparation of a medicine for promoting spinal cord injury repair. According to the invention, a bioinformatics and phosphorylated proteomics technology combined method is utilized to screen out that the functional protein of curcumin for promoting spinal cord injury repair is CLASP2 protein. The molecular biological technique verifies that curcumin regulates and controls microtubule stability by increasing phosphorylation of CLASP2 protein to promote functional repair after spinal cord transection injury. Therefore, the curcumin can regulate and control microtubules by promoting the phosphorylation of the CLASP2 to dynamically promote the motor function repair after the spinal cord injury of the mouse. The method has an important application value.

The invention discloses a rice grain type protein Os03g0762000 as well as a coding gene and application thereof. The rice grain type protein Os03g0762000 has an amino acid sequence as shown in SEQ ID NO.2, or is a functional equivalent variant formed by substitution, deletion or addition of one or more amino acid residues in the sequence as shown in SEQ ID NO.2; the coding gene has a nucleotide sequence as shown in SEQ ID NO.1, or a nucleotide sequence which is hybridized with SEQ ID NO.1 and codes the same functional protein. The invention discloses a method for cultivating a small-grain high-quality variety by using the gene, namely editing a specific position of the gene by a gene editing method so as to obtain a small-grain rice germplasm. The regulatory gene is good in regulation effect, compared with a wild type, the rice grain length and grain width are obviously reduced, and the characteristics of the regulatory gene show unique application value in rice mechanized hybrid breeding.

The invention discloses a mechanical response type engineered mesenchymal stem cell exosome preparation for treating diseases caused by fibrosis inflammation, a preparation method and application, and the exosome preparation is an exosome which is secreted by mesenchymal stem cells and has high cholesterol and high-efficacy protein. The exosome is prepared by culturing mesenchymal stem cells on a hydrogel matrix in a modulus hardness range of 1-50KPa, the exosome secretion amount of a single cell is (3-5) * 10 < 4 > / cell, the cholesterol content is (1-10) nM / 5mg exosome, and the size of the exosome is 110-140nm; the high-efficacy protein is a key functional protein for regulating and controlling the biological activity of the exosome and the cell action efficiency. The cholesterol content in the exosome is precisely regulated and controlled, the cytoplasm delivery efficiency and tissue penetrability of the exosome are improved, a whole-process control system of hydrogel preparation, stem cell culture and exosome purification is constructed, and the stability of the preparation and the yield of the exosome are improved.

The invention provides chickpea protein peptide as well as a preparation method and application thereof. The preparation method of the chickpea protein peptide provided by the invention comprises the following steps: sequentially carrying out enzymolysis on chickpea protein isolate by using neutral protease, pepsin and pancreatin; after the enzymolysis is finished, performing ultrafiltration on an enzymolysis product by using an ultrafiltration membrane with the interception amount of 3kDa, and collecting components with the molecular weight of less than 3kDa; and finally, carrying out gel chromatography on the component, and collecting the corresponding component to obtain the chickpea protein peptide. The chickpea protein peptide prepared by the method has inhibitory activity on DPP-IV, alpha-glucosidase and alpha-amylase, can effectively inhibit the activity of Caco-2 cell sucrase, maltase and DPP-IV, and has a certain inhibitory effect on glucose transport; therefore, the chickpea protein peptide provided by the invention has good hypoglycemic activity, and a new thought is provided for development of food-borne functional protein peptides.

Provided herein are compositions with enhanced protein content, compositions with functional proteins, protein combinations and methods for the preparation thereof.

The invention relates to the technical field of peanut functional protein structure application, in particular to a peanut protein amyloid fiber and a preparation method thereof. The method specifically comprises the following steps: (1) mixing peanut meal with an organic solvent, degreasing to obtain degreased peanut powder, and preparing the degreased peanut powder into a degreased peanut powder solution; (2) centrifuging the degreased peanut powder solution for the first time, taking a supernatant, adjusting the pH value, standing, centrifuging for the second time, taking a precipitate, dialyzing, and freeze-drying to obtain peanut protein isolate; (3) hydrating the peanut protein isolate, centrifuging for the third time, taking supernatant to obtain a peanut protein isolate solution, adjusting the pH value, centrifuging for the fourth time, taking supernatant, and filtering to obtain a protein solution; and (4) carrying out fibrosis treatment on the protein solution to obtain the peanut protein amyloid fiber. The preparation method provided by the invention can improve the generation amount of the peanut protein amyloid fiber and increase the length of the fiber, and provides a theoretical basis for the application of the peanut protein amyloid fiber in the food industry.

Methods of gene therapy are provided for treating or preventing age-related diseases or conditions by regulating one or more functional proteins associated with age-related diseases or conditions.

The invention relates to the technical field of protein modified fibers, and particularly discloses a functional protein composite cellulose fiber as well as a preparation method and application thereof. The invention relates to a preparation method of a functional protein composite cellulose fiber. The preparation method comprises the following steps: 1) uniformly mixing components including nano silicon dioxide coated zinc oxide particles and deionized water to prepare a ZnO / SiO2 mother solution; 2) uniformly mixing components including a cellulose raw material and a solvent to prepare a cellulose spinning solution; the solvent is composed of quaternary ammonium base and a cosolvent; 3, the ZnO / SiO2 mother solution and the cellulose spinning solution are taken and mixed, the mixture is injected into a coagulating bath to be coagulated and formed, and the functional protein composite cellulose fiber is obtained.The prepared functional protein composite cellulose fiber has the advantages of being low in ZnO / SiO2 wastage rate and good in compatibility and has the lasting deodorization, antibacterial and bacteriostatic functions.

A modular CRISPR-Cas system for ribonucleoprotein (RNP) delivery is provided, the CRISPR-Cas system comprising a CRISPR-Cas protein fused to a first binding peptide and a functional protein fused to a second binding peptide, where the first binding peptide and the second binding peptide are configured to bind to each other and define a binding peptide pair. Also provided are methods of assembling the CRISPR-Cas complexes for the delivery of ribonucleoprotein (RNP) and the CRISPR-Cas complexes, as well as the use of the modular CRISPR-Cas systems or CRISPR-Cas complexes for the modification of the genome of an organism.

Disclosed herein are computer-implemented systems and methods for generating functional protein sequences using a library of protein fragments.

The invention relates to the technical field of gene engineering, in particular to an atractylodes macrocephala terpene synthase gene AmTPS9 and a product coded by the same and application of the gene AmTPS9. The gene AmTPS9 has one of the following nucleotide sequences: (1) a nucleotide sequence as shown in SEQ ID NO.1; and (2) a nucleotide sequence which is obtained by substituting, deleting or adding one or more nucleotides to the nucleotide sequence as shown in SEQ ID NO.1 and expresses protein with the same function. According to the invention, a coding gene of an atractylodes macrocephala terpene synthase gene (AmTPS) is cloned from the rhizome of the atractylodes macrocephala, and the synthase can be applied to a pathway prepared by taking farnesyl pyrophosphate (FPP) as a substrate. By utilizing the gene provided by the invention, the content of terpenoids in bighead atractylodes rhizome can be increased through a genetic engineering technology.

The present invention relates to a protein-ribonucleic acid complex comprising (I) at least one multi-functional protein or a set of at least two proteins and (II) a nucleic acid editor complex, a polynucleotide encoding the multi-functional protein (I) and its uses in the delivery of nucleic acids and gene editing tools. The present invention also relates to a nucleic acid delivery system, a plurality of polynucleotides, pharmaceutical compositions, a cell or a cell line, and kits. The present invention further relates to medical uses of the nucleic acid delivery system or the cell or cell line. Also provided are uses of a cell or a cell line in therapeutic and diagnostic applications, as well as in tissue engineering. Finally, the present invention further relates to an in vitro or in vivo method of editing a nucleic acid molecule in a target cell, and a method for nucleic acid delivery.

The invention provides paeonia suffruticosa-derived phosphatidyldiacylglycerol acyltransferase 1-2 or a coding gene and application thereof, and belongs to the technical field of functional proteins. The invention relates to phosphatidyldiacylglycerol acyltransferase 1-2 from paeonia ostii, which comprises phosphatidyldiacylglycerol acyltransferase 1-2 from paeonia ostii and / or phosphatidyldiacylglycerol acyltransferase 1-2 from paeonia suffruticosa. Experiments show that the Paeonia ostii-sourced phosphatidyldiacylglycerol acyltransferase 1-2 and / or Paeonia fulva-sourced phosphatidyldiacylglycerol acyltransferase 1-2 not only can synthesize linoleic acid and alpha-linolenic acid by using exogenous polyunsaturated fatty acid, but also does not affect accumulation of yeast total lipid. The peony-sourced phosphatidyldiacylglycerol acyltransferase 1-2 provided by the invention provides a new method for biosynthesis of triacylglycerol, and provides a new way for genetic breeding of oil crops.