221 results about "Functional protein" patented technology

A method for functional antimicrobial peptide design is provided. The method includes running pretrained protein large language models as a generator and enhancing a sample candidate. The enhancing a sample candidate includes performing an automatic pipeline based on machine learning methods and a plurality of bioinformatics methods and is configured to perform protein inverse folding and computational protein sequence designing. The computational protein sequence designing includes running a pretrained deep learning-based protein structure model, an autoregressive pretrained protein sequence model, and a deep learning-based alignment model. The pretrained deep learning-based protein structure model is configured to learn three-dimensional structures of the proteins and output latent structure embeddings in a high dimensional space. The autoregressive pretrained protein sequence model includes a protein language model to automatically generate protein sequences and is trained to predict next amino acid in a protein sequence based on a preceding sequence(s) of amino acids.

The invention discloses a sea cucumber flower self-assembled polypeptide-selenium nano-carrier with antioxidant activity and a preparation method and application thereof, belongs to the technical field of functional protein peptides, and particularly relates to a preparation method of the sea cucumber flower self-assembled polypeptide-selenium nano-carrier with antioxidant activity, which comprises the following steps: heating a sea cucumber flower protein solution; the preparation method comprises the following steps: mixing sea cucumber with simulated gastric juice, reacting for 30-150 minutes, then adding simulated intestinal juice, reacting for 20-120 minutes, carrying out enzyme deactivation treatment, centrifuging, taking supernate, and freeze-drying to obtain sea cucumber flower self-assembled polypeptide; and mixing the sea cucumber flower self-assembled polypeptide with deionized water to prepare a sea cucumber flower self-assembled polypeptide solution, mixing the sea cucumber flower self-assembled polypeptide solution with a sodium selenite solution and a phosphate buffer solution, reacting for 20-60 minutes, washing with an ethanol solution, centrifugally collecting precipitate, and freeze-drying to obtain the sea cucumber flower self-assembled polypeptide-selenium nano-carrier. The sea cucumber flower self-assembled polypeptide-selenium nano-carrier prepared by the invention has good antioxidant activity and potential of effectively delivering selenium to an organism.

Embodiments disclosed herein provide methods, including computer-implemented methods, for designing guide sequence which may be incorporated into custom, large scale guide sequence libraries. The methods require only a list of target genes as input and utilize on target and off target scores to generate an optimal set of guide sequences for a set of target genes. In certain embodiments, the methods may also utilize multi-tissue RNA-sequencing data and / or protein annotation to design targets to genes that are highly expressed and / or contain a functional protein domain. The invention further comprises guide libraries, cells comprising said guide libraries. Computer-implemented embodiments further improve computer system function by reducing excessive user wait time through the use of data structures that reduce search from linear to logarithmic time.

A construction and use of an environmentally adaptive co-regulated mRNA nanodelivery system. By incorporating EACR molecules into mRNA nanoparticles, the microenvironment is remodeled to be suitable for robust and sustained mRNA expression, while tissue damage associated with the self-immunogenicity of mRNA drugs is avoided. The nanodelivery system is compatible with ionizable lipid nanoparticles, cationic liposomes, cationic nanoemulsions, and polymeric nanoparticles. The EACR molecules include: anti-inflammatory drugs, tyrosine kinases / adaptors, JAK / STAT and MAPK pathway inhibitors, nutrients and metabolites, membrane transporters / ion channels, phosphodiesterase and cellular stress inhibitors, and natural viral proteins. The therapeutic mRNAs may encode tumor, viral, or bacterial antigens, immunomodulatory factors, therapeutic antibodies, or functional proteins / enzymes, and can play a role in the fields of regenerative medicine, protein supplementation / replacement therapy, targeted gene editing, and immunotherapy.

The invention discloses a functional protein screening method based on a large language model, and belongs to the technical field of protein screening, and the method specifically comprises the following steps: obtaining a protein three-dimensional structure and functional literature, converting the protein three-dimensional structure and functional literature into a multi-modal feature vector by using an encoder, and constructing a domain-specific vector database; after a screening instruction is received, related feature data of semantic matching is called through a vector retrieval algorithm; then, inputting the data into a large language model to execute biological mechanism reasoning, and outputting an initial candidate protein sequence containing predicted activity; further, physical binding energy is calculated by using a molecular docking program, a deviation coefficient between the physical binding energy and the predicted activity is generated, and the coefficient is used as a penalty term to construct a dynamic correction function to update the scoring weight; and finally, performing secondary sorting on the candidate set based on the updated weight, and outputting the sequence sorted for the first time as a target protein. According to the method, AI reasoning and physical verification are effectively combined, and the screening accuracy is improved.

The invention discloses a mechanical response type engineered mesenchymal stem cell exosome preparation for treating diseases caused by fibrosis inflammation, a preparation method and application, and the exosome preparation is an exosome which is secreted by mesenchymal stem cells and has high cholesterol and high-efficacy protein. The exosome is prepared by culturing mesenchymal stem cells on a hydrogel matrix in a modulus hardness range of 1-50KPa, the exosome secretion amount of a single cell is (3-5) * 10 < 4 > / cell, the cholesterol content is (1-10) nM / 5mg exosome, and the size of the exosome is 110-140nm; the high-efficacy protein is a key functional protein for regulating and controlling the biological activity of the exosome and the cell action efficiency. The cholesterol content in the exosome is precisely regulated and controlled, the cytoplasm delivery efficiency and tissue penetrability of the exosome are improved, a whole-process control system of hydrogel preparation, stem cell culture and exosome purification is constructed, and the stability of the preparation and the yield of the exosome are improved.

The invention relates to the technical field of gene engineering, in particular to an atractylodes macrocephala terpene synthase gene AmTPS9 and a product coded by the same and application of the gene AmTPS9. The gene AmTPS9 has one of the following nucleotide sequences: (1) a nucleotide sequence as shown in SEQ ID NO.1; and (2) a nucleotide sequence which is obtained by substituting, deleting or adding one or more nucleotides to the nucleotide sequence as shown in SEQ ID NO.1 and expresses protein with the same function. According to the invention, a coding gene of an atractylodes macrocephala terpene synthase gene (AmTPS) is cloned from the rhizome of the atractylodes macrocephala, and the synthase can be applied to a pathway prepared by taking farnesyl pyrophosphate (FPP) as a substrate. By utilizing the gene provided by the invention, the content of terpenoids in bighead atractylodes rhizome can be increased through a genetic engineering technology.

The invention provides paeonia suffruticosa-derived phosphatidyldiacylglycerol acyltransferase 1-2 or a coding gene and application thereof, and belongs to the technical field of functional proteins. The invention relates to phosphatidyldiacylglycerol acyltransferase 1-2 from paeonia ostii, which comprises phosphatidyldiacylglycerol acyltransferase 1-2 from paeonia ostii and / or phosphatidyldiacylglycerol acyltransferase 1-2 from paeonia suffruticosa. Experiments show that the Paeonia ostii-sourced phosphatidyldiacylglycerol acyltransferase 1-2 and / or Paeonia fulva-sourced phosphatidyldiacylglycerol acyltransferase 1-2 not only can synthesize linoleic acid and alpha-linolenic acid by using exogenous polyunsaturated fatty acid, but also does not affect accumulation of yeast total lipid. The peony-sourced phosphatidyldiacylglycerol acyltransferase 1-2 provided by the invention provides a new method for biosynthesis of triacylglycerol, and provides a new way for genetic breeding of oil crops.

The application discloses a method for preparing mesoporous zeolite under normal temperature and pressure and application thereof, and the preparation method comprises the following steps: (1) preparation of a functional protein biomodule: cationic polypeptide is loaded on yeast to obtain a biomodule aqueous solution; (2) deposition of silicon and aluminum: the pH of the biomodule solution obtained in the step (1) is adjusted to neutral, then a silicon source, an aluminum source and a titanium source are added into the solution, and the solution is incubated on a shaking table for 3 days; (3) calcination: the solid obtained in the step (2) is collected by centrifugation, washed with ethanol and water alternately for 3 times, and calcined at 500 DEG C for 4h under an air atmosphere to obtain a zeolite molecular sieve adsorption material. The application can prepare porous molecular sieve small balls by using a simple method, and can provide a brand-new thought for synthesizing molecular sieve materials.

The present invention relates to a rep plasmid comprising at least one long adeno-associated virus replication protein coding sequence encoding at least one long functional rep protein, at least one short adeno-associated virus replication protein coding sequence encoding at least one short functional rep protein, and at least one heterologous element, wherein following translation the at least one heterologous element results in a ratio of long adeno-associated virus rep protein to short adeno-associated virus rep protein of about 1:5 to about 1:5000.

The application belongs to the field of new application of functional proteins and mutants thereof, and discloses application of sugarcane ScCBS protein and key domain deletion mutants thereof. The nucleotide sequence of the sugarcane ScCBS protein is shown as SEQ ID NO:1, and the amino acid sequence is shown as SEQ ID NO:2. The key domain deletion mutants of the ScCBS protein are ScCBS△motif1, ScCBS△motif3, ScCBS△motif4 and ScCBS△motif9, which respectively lack 28, 50, 49 and 29 amino acids. The application proves that the ScCBS protein can interact with SCSMV P1 and inhibit the RNA silencing suppressor activity by means of yeast two-hybrid, two-molecular fluorescence complementary experiment and agrobacterium transient expression system; the four mutants all lose the interaction and inhibition activity. Therefore, the ScCBS protein and the key domain deletion mutants thereof can be used for cultivating excellent SCSMV-resistant materials, and have wide application prospects in sugarcane disease-resistant molecular breeding.

The invention belongs to the technical field of agricultural biology, and provides a soybean nodulation and nitrogen fixation related gene and application of an encoding protein thereof, the nucleotide sequence of the gene Gm02g072800 is shown as SEQ ID NO: 1, or a variant sequence which encodes the same functional protein and has homology greater than or equal to 90%; the amino acid sequence of the protein coded by the gene Gm02g072800 comprises a conservative AP2 structural domain, belongs to an ERF transcription factor family, is positioned in a cell nucleus, and has the functions of promoting soybean root nodule development and symbiotic nitrogen fixation. According to the invention, the specific expression of the gene in the soybean middle column and root nodule endodermis is determined, and a target gene is provided for cultivating soybean varieties with high nitrogen fixation efficiency.

The application provides a combined drug, a liposome, a biomimetic liposome, a preparation method of the combined drug, the liposome and the biomimetic liposome, and application of the combined drug, the liposome and the biomimetic liposome in preparation of an anti-breast cancer lung metastasis drug, and belongs to the technical field of biological medicines. The combined drug comprises panatinib and ginsenoside Rg3. The application further provides a liposome PNT-Rg3-Lipo for simultaneously loading the two, and a biomimetic liposome MM / PNT-Rg3-Lipo coated with a macrophage membrane. Experiments show that the combination of the two can synergistically regulate chemokines CXCL1 , CXCL2 and CXCL11 , and the effect is better than the sum of the single use. The MM / PNT-Rg3-Lipo has uniform particle size, retains integrin alpha 4 / beta 1 functional proteins, can target lung metastases, significantly reduces metastatic nodules, and has good biocompatibility. The application provides an effective new strategy for resisting breast cancer lung metastasis.

The invention relates to a helper adenovirus genome wherein expression of one or more of the adenoviral early E1 genes, E2 genes including DNA-binding protein (DBP), pre-terminal protein (pTP) and DNA polymerase, or production of functional proteins are regulated. The invention also relates to a process for delivery of the helper-dependent or gutless adenovirus vector genome and modulating replication of the helper virus DNA in the manufacturing cells, in which to allow a sufficient length of time before excision, cutting, or recombination to removal of the viral packaging signal DNA sequence from the helper adenovirus genome prior to induction of viral DNA replication, and expression of helper proteins that are required for adenovirus capsid particle formation and packaging, or encapsidation, of the helper-dependent or gutless adenovirus vector genomes, while preventing the packaging, or encapsidation, of the helper adenoviral genome into preform adenoviral capsids.

The invention discloses high-solubility and high-stability protein powder containing an acidic functional component. The protein powder comprises the following components in percentage by mass: 70-92% of soybean protein isolate, 1-3% of the acidic functional component and 4.52-16.5% of a composite dissolution-promoting dispersion-stabilizing component, the composite dissolution-promoting and dispersion-stabilizing component is prepared from the following components in percentage by mass: 3 to 10 percent of isomaltooligosacharide, 1.5 to 6 percent of fructo-oligosaccharide and 0.02 to 0.5 percent of stevioside; the invention further discloses a preparation method of the high-solubility and high-stability protein powder containing the acidic functional components. The invention further discloses an acidic functional protein beverage and a preparation method thereof. The brewing dissolution speed and the solution stability are remarkably improved through an oligosaccharide-sweetening agent compounding system, the dry mixing process is simple, complex modification treatment is not needed, the cost is lower than that of an enzymolysis or microcapsule technology, and the good industrialization advantage is achieved.

The invention relates to the field of plant genetic engineering, and discloses a corn sheath blight disease-resistant gene GRMZM2G057329 and application thereof, and the corn sheath blight disease-resistant gene GRMZM2G057329 comprises any one of the following sequences: a nucleotide sequence shown as SEQ ID No.1, a nucleotide sequence shown as SEQ ID No.2, a nucleotide sequence shown as SEQ ID No.3, a nucleotide sequence shown as SEQ ID No.4, a nucleotide sequence shown as SEQ ID No.5, a nucleotide sequence shown as SEQ ID No.6, a The nucleotide sequence is as shown in SEQ ID No.2; the amino acid sequence of the protein is shown as SEQ ID No.3; the nucleotide sequence has 90% or more of identity with the nucleotide sequence as shown in SEQ ID No. 1 or SEQ ID No. 2 and is used for coding protein with the same function as the nucleotide sequence as shown in SEQ ID No. 1 or SEQ ID No. 2. According to the present invention, the DNA fragment of the complete coding segment of the anti-disease gene GRMZM2G057329 separated and cloned from corn is adopted, and the GRMZM2G057329 gene expression quantity is increased so as to significantly enhance the sheath blight resistance of the corn genetic transformation plant;

The invention belongs to the field of microorganisms and medicine health care, and relates to an ackermania muciniphila strain and application thereof, the preservation number of the ackermania muciniphila strain is CGMCC (China General Microbiological Culture Collection Center) No.34370, and a 16SrDNA (Deoxyribose Nucleic Acid) sequence of the ackermania muciniphila strain has 93% similarity with a sequence shown as SEQ ID NO: 1. The new ackermania muciniphila strain (AKKBC300) is separated from a healthy human body and has good safety; good tolerance to low pH value and bile salt is shown in vitro, and it is indicated that the compound can reach the intestinal tract through the upper digestive tract and play a role; according to the present invention, genomics analysis results prove that the bacterial strain contains the gene Akkermansia BC00573 with high homology with the known probiotic functional protein Amuc1100, such that the molecular basis is provided;

The present disclosure relates to the field of protein detection technology, and discloses a protein detection method based on a combined nanopore structure, comprising: a surface of a sandwich film chip is processed to form a nano-through hole, and a “hole-cavity-hole” structure is prepared. The “hole-cavity-hole” structure is driven by the electric field of the functional protein to detect the protein. The present disclosure adopts the above-mentioned protein detection method based on a combined nanopore structure, which can effectively overcome the problem of protein detection reading length caused by the limitation of nanopore space. Meanwhile, in contrast to methods that embed functional protein complexes within a phospholipid bilayer, integrating functional proteins directly into solid nanopores combines the advantages of both biological and solid systems. This approach not only relaxes the stringent environmental demands placed on the sensor but also enhances its resolution and stability.

This invention discloses a method and application for efficient synthesis of human lactoferrin through multi-copy integration, relating to the fields of genetic engineering and bioengineering. The invention provides a method for synthesizing human lactoferrin, including the steps of fermenting and culturing a recombinant Aspergillus niger strain, followed by isolation and purification to obtain human lactoferrin; the construction method of the recombinant Aspergillus niger strain includes the step of integrating the hLF gene into a gene locus in the host strain genome to construct the recombinant Aspergillus niger strain. This invention constructs a recombinant Aspergillus niger strain capable of recombinantly expressing human lactoferrin, initiating protein expression through a strongly inducible promoter, fusing endogenous high-secretion proteins, and optimizing the multi-copy integration method to integrate the lactoferrin expression cassette, achieving high-level synthesis of human lactoferrin, laying the foundation for the industrial production of lactoferrin or high-density fermentation of other food functional proteins using Aspergillus niger.

Provided are a nucleic acid construct for achieving modular loading of an engineered EVs functional protein and use of said construct. A modular design principle is adopted, specific modules are selected for combination, a mutant sequence is optimized and screened to obtain an improved nucleic acid construct, and finally, the engineered EVs modularly loaded with functional protein is converted and expressed. The product has the following advantages that: I) the modular loading of the engineered EVs functional protein is achieved; 2) the loading density and the loading efficiency of the protein inside and outside an EVs membrane are improved; and 3) a specific support sequence module is selected to avoid enzyme digestion. The construct is used to prepare the engineered EVs, so that the modular loading of the functional protein can be achieved, the loading efficiency is improved, and the construct has a wide clinical application value and market prospect.

The present disclosure relates to methods, devices and systems for exposing microorganisms or compositions comprising functional proteins to an oscillating electric field substantially free of a magnetic component in order to improve stress responses, survival and metabolite or protein production. In embodiments, a controller drives one or more insulated emitters with a true AC signal or pulsed DC signal, in stepped, swept or shuffled patterns of an electric field oscillating at one or more frequencies in the range 210 Hz to 99 kHz. Exposure to the field provides surmounting energy that enhances protein breathing, catalysis, and fundamental functions of multiprotein complexes including transcription factors and ribosomes, thereby improving stress resistance of microorganisms, their viability during fermentation, drying, storage, transport, and / or increasing production of functional or therapeutic proteins, metabolites or microbial lysates. The technology is applicable to manufacture of probiotics, therapeutics, metabolites, food, alcohol, feed and bioenergy, cosmetics, nutraceuticals and biopharmaceuticals..

The invention discloses a preparation method and application of sheep small intestine immunomodulatory protein, and belongs to the field of functional protein preparation. The preparation method comprises the following steps: carrying out phosphate buffer low-temperature low-salt extraction on freeze-dried powder of small intestines of sheep, carrying out gel column chromatography and anion exchange column chromatography separation and purification, and further carrying out high performance liquid chromatography analysis and activity evaluation to obtain the protein component with immunoregulatory activity. The sheep small intestine protein prepared by the method has no cytotoxicity, can promote the proliferation of macrophages RAW264.7 and enhance the NO release capability of cells, has an obvious promotion effect on the secretion of TNF-alpha and IL-6 by the cells, and has relatively high immunoregulation activity, so that a scientific basis is provided for the application of the sheep small intestine protein in the development of functional foods and medicines for enhancing immunoregulation; the development and utilization value of livestock by-products is improved.

The present disclosure relates to compositions for the restoration of porosome faction, comprising isolated porosome complexes that include a functional CFTR protein and a pharmaceutically acceptable excipient. These compositions are specifically formulated for inhaled or nasal administration, enabling delivery of the functional porosome complexes directly to the airway epithelium. By restoring the presence of functional CFTR within the porosome complex at the plasma membrane, the disclosed compositions address the underlying secretory defect in cystic fibrosis and provide a novel therapeutic approach for patients affected by this disease.

The invention discloses a protein nanoparticle-nanofiber composite material and a preparation method thereof, and belongs to the technical field of food processing and functional protein materials. The method comprises the following steps: (1) taking fresh egg white, diluting and stirring to obtain uniform egg white protein dispersion liquid; (2) performing acylation modification on the egg white protein dispersion liquid to increase surface negative charges and improve conformation flexibility; (3) performing controllable enzymolysis on the acylated egg white protein, so that part of peptide chains of the acylated egg white protein are broken and oligopeptide with self-assembly capability is formed; and (4) carrying out heat treatment on the enzymolysis product, inducing the peptide fragment to be self-assembled to form nano-particles while inactivating the protease through heat treatment, further forming a nano-fiber network dominated by a beta-folding structure, and finally obtaining the protein nano-particle-nano-fiber composite system. Through the synergistic effect of acylation, enzymolysis and heat treatment, accurate conversion of protein from a natural spherical conformation to a functional nanostructure is achieved, and the obtained composite material has the dispersion stability of particles and the network enhancement effect of fibers and has excellent foamability, emulsibility and gelling property.

The application discloses a method for collecting and purifying mouthpart secretion of a predatory natural enemy Orius minor, and belongs to the technical field of biology. The method comprises the following steps: constructing a double-layer membrane sandwich artificial feeding device, encapsulating a nutrient solution in a sandwich space between a first membrane layer and a second membrane layer, and using the feeding drive of the Orius minor to make the Orius minor actively pierce the membrane layer and feed the nutrient solution, so that the mouthpart secretion is released into the nutrient solution in the natural feeding process; after the nutrient solution containing the secretion is collected, ultrafiltration centrifugation technology is used for concentration and purification, and a high-purity mouthpart secretion protein sample is obtained. The natural feeding state of the Orius minor is simulated, the collected secretion can more truly reflect the functional protein released by the Orius minor in the predation process, meanwhile, the integrated design of collection and purification effectively removes small molecular impurities in the sample, and the sample can be directly used for high-precision analysis such as proteomics.

The invention discloses a preparation method for relieving cracks and white edges of quick-frozen dumpling wrappers based on vital wheat gluten antifreeze protein, and relates to the technical field of wheat deep processing, and the preparation method comprises the following steps: preparing GAFP; determining free amino acids; observing an ice crystal structure; carrying out hydrophilic analysis; making dumpling wrappers, and performing freeze thawing treatment; analyzing the influence of GAFP on the moisture content of the dumpling wrappers; the antifreeze protein and the antifreeze polypeptide are prepared by taking wheat processing byproduct vital gluten as a raw material and adopting an enzyme method, so that the antifreeze protein and the antifreeze polypeptide have a protection effect on quick-frozen dumpling wrappers in a freezing and thawing period, a theoretical basis is provided for developing wheat source functional protein products, high-valued application of wheat processing byproducts is promoted, a wheat industrial chain is prolonged, and the antifreeze protein has important economic and social significance and has a good application prospect. The anti-freezing protein and the anti-freezing polypeptide GAFP are prepared from wheat processing by-product vital wheat gluten as a raw material by adopting an enzyme method, and the anti-freezing protein and the anti-freezing polypeptide GAFP have a protection effect on quick-frozen dumpling wrappers in a freezing and thawing period, so that the cracking and white edge prevention effects of the quick-frozen dumplings are improved, and the mouth feel of consumers is achieved.

The application belongs to the field of biological detection, and particularly relates to a specific monoclonal antibody of an AKK bacterial efficacy protein Amuc_1100, a kit, a detection method and application thereof. The antibody pair is 3E10 and 2F7 monoclonal antibodies, the heavy chain and light chain variable region amino acid sequences of 3E10 are SEQ ID NO. 2 and SEQ ID NO. 4, and the heavy chain and light chain variable region amino acid sequences of 2F7 are SEQ ID NO. 6 and SEQ ID NO. 8. The antibody pair has high specificity and high affinity, a colloidal gold rapid detection kit and a double antibody sandwich ELISA quantitative detection kit based on the antibody pair realize rapid screening and accurate quantification of the Amuc_1100 protein, have good linearity, high precision and excellent accuracy, and can be used for detection of the protein in mucoid Akkermansia and quality control and activity evaluation of related probiotic preparations, thereby providing key technical support for related basic research and industrial application.

A variable splicing event in a gene can result in a non-productive mRNA transcript, which in turn can result in abnormal protein expression, and a therapeutic agent that can target a variable splicing event in a gene can modulate the expression level of a functional protein and / or inhibit abnormal protein expression in a patient. Such therapeutic agents are useful for the treatment of conditions or diseases caused by protein deficiency and / or mitochondrial function deficiency.

The invention relates to the technical field of gene engineering, in particular to an Atractylodes lancea terpene synthase gene AlTPS42, a product coded by the Atractylodes lancea terpene synthase gene AlTPS42 and application of the Atractylodes lancea terpene synthase gene AlTPS42, and the Atractylodes lancea terpene synthase gene AlTPS42 has a nucleotide sequence as shown in SEQ ID NO.1 or a nucleotide sequence which is obtained by substituting, deleting or adding one or more nucleotides to the nucleotide sequence as shown in SEQ ID NO.1 and expresses protein with the same function. The enzyme can be applied to a pathway prepared by taking farnesyl pyrophosphate (FPP) as a substrate. By utilizing the gene provided by the invention, the content of the terpenoid substances of the atractylodes lancea can be increased through a genetic engineering technology.