456 results about "Functional protein" patented technology

To provide constructs, vectors, relative host cells and pharmaceutical compositions which allow an effective gene therapy, in particular of genes larger than 5Kb.SOLUTION: The present invention implements cellular large protein reconstitution by providing to a target cell two or more fragments of large protein fused to split inteins to promote intein-mediated trans-splicing and reconstitute the functional protein.SELECTED DRAWING: None

The invention discloses a recombinant XII type collagen as well as a preparation method and application thereof. The amino acid sequence of the recombinant XII type collagen is any one of (A1)-(A3); (A1) is an amino acid sequence as shown in SEQ ID NO: 1; (A2) is an amino acid sequence which is obtained by substitution, deletion or insertion of one or more amino acid residues of the amino acid sequence as shown in SEQ ID NO: 1 and has the same functional protein; and (A3) is an amino acid sequence of a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of the sequence (A1) or (A2). The recombinant XII type collagen provided by the invention has good antioxidant activity, and has a repairing effect on the problems of skin laxity, wrinkle increase and the like caused by degradation of type I collagen fibers of aged skin.

The invention relates to the technical field of protein purification, in particular to a biological method for purifying fusion protein through two different affinity tags. Two affinity purification tags and a dissolution promoting tag are perfectly combined, so that soluble expression of recombinant protein is ensured, high-purity target protein can be obtained through a two-step mild and efficient affinity purification process, the function and activity of the protein are ensured, a complex'capture-medium purification-fine purification 'multi-step purification process is avoided, and the method is suitable for industrial production. The purification process is shortened, the purification time is shortened, the purification efficiency is improved, and the method can be used for production and purification of functional proteins.

The invention discloses a method for analyzing the tumor microenvironment of esophageal squamous cell carcinoma. The method comprises the following steps: selecting a basic tissue structure protein to be analyzed in a tumor microenvironment, carrying out cell segmentation and protein expression level extraction, carrying out clustering identification on cell types, identifying a three-level lymph structure and a neighborhood thereof, and determining an immune cell proportion and an expression level of a key functional protein. Identifying tissue regions and neighborhoods thereof and determining different cell proportions and expression levels of key functional proteins, as well as determining cell-cell spatial interactions in the tumor microenvironment. The method provided by the invention can greatly improve the efficiency and accuracy of predicting the intervention effect.

The invention belongs to the technical field of biological medicines, and particularly relates to a sperm injury repair model and an establishment method thereof. Comprising the following steps: step 1, establishing a sperm lead poisoning model; and 2, establishing a lead poisoning sperm repair model. The method for establishing the lead poisoning sperm repair model in the step 2 comprises the following steps: (1) preparing a preparation for optimizing sperm functions in an extracellular vesicle way; (2) repairing the damaged sperms by using the preparation for optimizing the sperm function through the extracellular vesicles; and (3) detecting the activity of the repaired sperms. In the invention, by establishing a sperm injury repair model and adopting an extracellular vesicle modification technology, the key functional protein is introduced into the extracellular vesicle, and the method has important significance on auxiliary researches such as development and research of related drugs for improving sperm injury and improving sperm motility of asthenospermia patients in vitro.

Provided herein are methods for treating conditions and disease characterized by SCN1A, SCN8A or SCN5A protein deficiency by targeting the alternative splicing events in the SCN1A gene and modulating the expression level of functional proteins in Dravet Syndrome patients and / or inhibit aberrant protein expression.

The invention discloses a group of mitochondrial signal peptides and application thereof, and a group of signal peptides with a mitochondrial targeting function is obtained for the first time by screening endogenous mitochondrial key functional proteins of rhodotorula. The invention further realizes expression of a target gene through mitochondrial signal peptide mediation, realizes expression of a target product synthetic pathway gene in rhodotorula mitochondria, and successfully constructs synthetic pathways of terpene such as limonene, linalool, patchouli alcohol and kaurene, branched chain alcohol such as isobutanol and organic acid such as 3-hydracrylic acid. Further, carbon metabolic flow of pyruvic acid flowing to the target product is dredged, and synthesis of the mitochondrial region indoor target product is finally realized. The characterization of a group of rhodotorula mitochondrial signal peptides disclosed by the invention can realize the synthesis of mitochondrial regional chamber target products, and lays a foundation for the research on the realization of metabolic engineering transformation in subcellular organelles.

Provided herein are methods for treating conditions and diseases characterized by SCN1A, SCN8A or SCN5A protein deficiency by targeting the alternative splicing events in SCN1A gene and modulating the expression level of functional proteins in Dravet Syndrome patients and / or inhibit aberrant protein expression.

Agents that target a processed mRNA, e.g., the 5′ UTR of the processed mRNA, can modulate protein expression, e.g., via modulation of translation of the processed mRNA. Alternative splicing events in genes can lead to non-productive mRNA transcripts which in turn can lead to aberrant protein expression. Agents that target the alternative splicing events in genes can modulate the expression level of proteins. Therapeutic agents, which can modulate protein expression by targeting a processed mRNA and / or alternative splicing events, can promote functional protein expression in patients and / or inhibit aberrant protein expression. Such therapeutic agents can be used to treat a condition or disease associated with protein deficiency and / or mitochondrial function deficit.

The invention provides a plug-and-play Mi3 protein nanocage fusion protein with a VNP6 tag and a preparation method and application of the plug-and-play Mi3 protein nanocage fusion protein, the fusion protein comprises the VNP6 tag, a SpyCatch003 component, a Mi3 protein nanocage and a functional protein sequence, and the VNP6 tag is located at the N end of the fusion protein. The VNP6 peptide sequence or the variant thereof is utilized to induce a vesicle or vesicle-like structure beneficial to protein folding and stabilization in escherichia coli, so that the correct folding rate and activity of recombinant protein are improved while the overall intracellular molecular crowding effect is relieved. The core idea of the invention lies in that the internal structure of cells is regulated at low temperature, and the internal microenvironment of an escherichia coli expression system is optimized, so that protein molecules under high expression load can obtain more reasonable spatial distribution, and the problems of misfolding and aggregation caused by molecular crowding are reduced.

The invention provides an oxygen-carrying protein molecule and a preparation method and application thereof, and relates to the technical field of functional protein preparation, the oxygen-carrying protein molecule comprises at least one cysteine part and at least one histidine part, the cysteine part comprises acetyl or ethyl maleoyl, and the histidine part comprises histidine. The histidine moiety includes a benzimidazole derivative group. The benzimidazole derivative is adopted as an auxiliary molecule and participates in the modification process of the sulfydryl modified compound on the hemoglobin, the modification site selectivity is improved, the conformational change is reduced, and the modification degree is accurately controlled, so that the oxygen binding force P50 of the modified hemoglobin is effectively improved.

The invention relates to a codon optimization method for improving the protein expression quantity of a target gene in a plant, and belongs to the technical field of gene engineering. The invention provides a codon optimization method for improving the protein expression quantity of a target gene in a plant, which comprises the step of replacing the third basic group of a codon of a target protein coding sequence with C or G to obtain an optimized target protein coding sequence. The method has effectiveness and universality in plants, and research shows that after codon optimization of the method, the protein expression quantity of target genes in a plant protein transient expression system and a transgenic plant is increased by several times to dozens of times. According to the production application of the functional protein taking the plant as the bioreactor, the yield of the target protein can be increased, and the protein purification cost can be reduced; according to the application of the method in preparation of the transgenic plant, the expression quantity of the target gene can be increased, so that the target character of the transgenic plant is enhanced, and the screening cost of the high-expression transgenic plant is reduced.

A method for functional antimicrobial peptide design is provided. The method includes running pretrained protein large language models as a generator and enhancing a sample candidate. The enhancing a sample candidate includes performing an automatic pipeline based on machine learning methods and a plurality of bioinformatics methods and is configured to perform protein inverse folding and computational protein sequence designing. The computational protein sequence designing includes running a pretrained deep learning-based protein structure model, an autoregressive pretrained protein sequence model, and a deep learning-based alignment model. The pretrained deep learning-based protein structure model is configured to learn three-dimensional structures of the proteins and output latent structure embeddings in a high dimensional space. The autoregressive pretrained protein sequence model includes a protein language model to automatically generate protein sequences and is trained to predict next amino acid in a protein sequence based on a preceding sequence(s) of amino acids.

The invention relates to the field of breeding technical equipment, and particularly provides protein expression profile screening and application of anxiety disorder of Alzheimer's disease. Specifically, for Alzheimer's disease anxiety disorder caused by amyloid beta protein accumulation, proteomics analysis finds that inhibition of aBLA-vCA1 pathway functions can effectively relieve social avoidance behaviors caused by amyloid beta protein accumulation; and the differential functional proteins of the anxiety disorder of the Alzheimer's disease, such as RPS6, UBQLN1, Eras2, Rpl30, SERBP1, CLU, APOE, C4B, C1QB, C1QA, C1QC, MAPK1, MAPK3, RHOG, PTK2B, ASPA, GAD1, GAD2, H3f3a, H4C8 and TSPYL4, are screened out. The different functional proteins can be used as biomarkers for detecting Alzheimer's disease anxiety disorder caused by accumulation of pulverulent beta protein, and can be used as potential therapeutic targets.

The invention provides a fusion protein for promoting proliferation of hair follicle stem cells as well as a preparation method and application of the fusion protein. The fusion protein is formed by fusion expression of a plurality of collagen structural domains capable of being combined with cell integrin and / or specific structural domains from certain functional proteins screened from collagen XVII and III type collagen. Nucleic acid molecules for coding the fusion protein are subjected to codon optimization and then recombined and stably expressed in escherichia coli to obtain the fusion protein. The fusion protein has excellent performance of promoting proliferation and migration of human hair follicle stem cells and human fibroblasts, can be used for repairing skin and hair follicle tissues and improving resistance of skin and hair follicles, and is suitable for popularization and application in the fields of beauty and skin care, medical equipment, tissue engineering and the like.

The invention discloses a functional protein mining and screening method and device, which combines multi-dimensional bioinformatics analysis such as protein structure prediction, protein function annotation, protein cluster mining and the like with an artificial intelligence method based on a protein big language to construct a process for mining and screening a functional protein. Protein structure analysis is introduced, and proteins with similar functions are explored by utilizing association of protein structures and functions and constructing a structure similarity map. The protein large language model can contain characteristic parameters related to protein functions, such as a protein structure, post-translational modification and biophysical characteristics, and reveals the inherent functional relevance of the protein in a vector matrix form. By introducing the two analysis means and combining with the traditional sequence-based protein function annotation method, the sequence similarity and affinity are lower, but proteins with corresponding functions are excavated and screened out, so that biomacromolecules can be utilized more efficiently and accurately.

The invention relates to a gene GL8 for controlling grain shape, appearance quality or / and yield of rice. The gene has (a) a nucleotide sequence as shown in SEQ ID No: 1; and (b) a nucleotide sequence which is generated by adding, substituting or deleting one or more basic groups from the nucleotide sequence as shown in SEQ ID No: 1 and is used for coding a functional protein for controlling the grain shape, the appearance quality and the yield of rice. The invention belongs to the technical field of gene engineering. By overexpressing the gene, the grain shape, appearance quality and yield of rice can be regulated and controlled.

The invention provides a BoNIA2b gene, which has a nucleotide sequence as shown in SEQ ID No.1, or a nucleotide sequence which is obtained by substituting, deleting and / or adding one or more nucleotides to the nucleotide sequence as shown in SEQ ID No.1 and expresses proteins with the same function, wherein the nucleotide sequence is obtained by substituting, deleting and / or adding one or more nucleotides to the nucleotide sequence as shown in SEQ ID No.1; the invention also provides an application thereof. Compared with the prior art, the broccoli BoNIA2b gene has the following advantages that functional verification is carried out on the broccoli BoNIA2b gene, it is found that overexpression of the BoNIA2b gene can promote increase of the content of glucoraphanin and sulforaphane in the hairy roots of broccoli, and it is found through further research that the content of glucoraphanin and sulforaphane in the hairy roots of broccoli can be increased; the overexpressed BoNIA2b gene is mainly used for regulating and controlling the expression level of some synthetic genes of glucoraphanin and sulforaphanin of the hairy roots of broccoli so as to regulate and control the synthesis of glucoraphanin and sulforaphanin. Finally, the BoNIA2b gene is proved to positively regulate the synthesis reaction of glucoraphanin and sulforaphane of broccoli.

The invention discloses a sea cucumber flower self-assembled polypeptide-selenium nano-carrier with antioxidant activity and a preparation method and application thereof, belongs to the technical field of functional protein peptides, and particularly relates to a preparation method of the sea cucumber flower self-assembled polypeptide-selenium nano-carrier with antioxidant activity, which comprises the following steps: heating a sea cucumber flower protein solution; the preparation method comprises the following steps: mixing sea cucumber with simulated gastric juice, reacting for 30-150 minutes, then adding simulated intestinal juice, reacting for 20-120 minutes, carrying out enzyme deactivation treatment, centrifuging, taking supernate, and freeze-drying to obtain sea cucumber flower self-assembled polypeptide; and mixing the sea cucumber flower self-assembled polypeptide with deionized water to prepare a sea cucumber flower self-assembled polypeptide solution, mixing the sea cucumber flower self-assembled polypeptide solution with a sodium selenite solution and a phosphate buffer solution, reacting for 20-60 minutes, washing with an ethanol solution, centrifugally collecting precipitate, and freeze-drying to obtain the sea cucumber flower self-assembled polypeptide-selenium nano-carrier. The sea cucumber flower self-assembled polypeptide-selenium nano-carrier prepared by the invention has good antioxidant activity and potential of effectively delivering selenium to an organism.

The invention relates to an industrial method, in particular to a method for preparing a universal functional protein nano-film on the surface of a base material, which comprises the following steps: (1) mixing a protein solution, an optional functional monomer and a photoinitiator solution to prepare a mixed solution; (2) soaking a base material in the mixed solution; and (3) irradiating with ultraviolet rays to obtain the protein nano-film on the surface of the base material. According to the universal method for continuously preparing the functional protein nano-film on the surface of the base material, rapid self-assembly aggregation and monomer polymerization of the protein can be carried out at the same time under ultraviolet induction, and the protein-functional polymer two-dimensional nano-film prepared on the surface of the base material is compact, low in roughness, high in transparency and good in application prospect. The biocompatibility is good.

The invention discloses a recombinant protein and application thereof in preparation of sodium alginate fibers. The recombinant protein is obtained by fusion expression of a functional protein and at least one alginic acid binding domain protein. The amino acid sequence of the alginic acid binding domain protein is as shown in at least one of SEQ ID No. 1, SEQ ID No. 2 and SEQ ID No. 3. The recombinant spider silk protein with the alginic acid CBM region can exert the material science characteristics in a sodium alginate-calcium ion system, the mechanical property of alginic acid fiber is greatly enhanced, and meanwhile, the biological activity of protein can be provided for an alginate fiber system.

Embodiments disclosed herein provide methods, including computer-implemented methods, for designing guide sequence which may be incorporated into custom, large scale guide sequence libraries. The methods require only a list of target genes as input and utilize on target and off target scores to generate an optimal set of guide sequences for a set of target genes. In certain embodiments, the methods may also utilize multi-tissue RNA-sequencing data and / or protein annotation to design targets to genes that are highly expressed and / or contain a functional protein domain. The invention further comprises guide libraries, cells comprising said guide libraries. Computer-implemented embodiments further improve computer system function by reducing excessive user wait time through the use of data structures that reduce search from linear to logarithmic time.

A construction and use of an environmentally adaptive co-regulated mRNA nanodelivery system. By incorporating EACR molecules into mRNA nanoparticles, the microenvironment is remodeled to be suitable for robust and sustained mRNA expression, while tissue damage associated with the self-immunogenicity of mRNA drugs is avoided. The nanodelivery system is compatible with ionizable lipid nanoparticles, cationic liposomes, cationic nanoemulsions, and polymeric nanoparticles. The EACR molecules include: anti-inflammatory drugs, tyrosine kinases / adaptors, JAK / STAT and MAPK pathway inhibitors, nutrients and metabolites, membrane transporters / ion channels, phosphodiesterase and cellular stress inhibitors, and natural viral proteins. The therapeutic mRNAs may encode tumor, viral, or bacterial antigens, immunomodulatory factors, therapeutic antibodies, or functional proteins / enzymes, and can play a role in the fields of regenerative medicine, protein supplementation / replacement therapy, targeted gene editing, and immunotherapy.

The invention discloses a protective agent as well as a preparation method and application thereof. The protective agent is prepared from inulin, fructo-oligosaccharide and erythritol. According to the protective agent provided by the invention, the inulin, the fructo-oligosaccharide and the erythritol are compounded to form a mixed protective agent, so that the heat resistance of functional protein in the bovine colostrum processing process can be improved, and the protective agent has a good protective effect on immune globulin (Ig-G) and lactoferrin (LF) in the processing process. Moreover, the three components all have probiotic effects, so that the development of high-value bovine coloctrum functional products is facilitated, and the comprehensive benefits of the bovine coloctrum are improved.

The invention discloses a functional protein screening method based on a large language model, and belongs to the technical field of protein screening, and the method specifically comprises the following steps: obtaining a protein three-dimensional structure and functional literature, converting the protein three-dimensional structure and functional literature into a multi-modal feature vector by using an encoder, and constructing a domain-specific vector database; after a screening instruction is received, related feature data of semantic matching is called through a vector retrieval algorithm; then, inputting the data into a large language model to execute biological mechanism reasoning, and outputting an initial candidate protein sequence containing predicted activity; further, physical binding energy is calculated by using a molecular docking program, a deviation coefficient between the physical binding energy and the predicted activity is generated, and the coefficient is used as a penalty term to construct a dynamic correction function to update the scoring weight; and finally, performing secondary sorting on the candidate set based on the updated weight, and outputting the sequence sorted for the first time as a target protein. According to the method, AI reasoning and physical verification are effectively combined, and the screening accuracy is improved.

The invention belongs to the technical field of cell bionic nano-drugs, and particularly discloses a cell bionic nano-particle as well as a construction method and application thereof. And performing simple ultrasonic fusion on the target cell membrane and the hydrophobic nano-core to obtain the cell bionic nano-particles. The bionic nano-cell can effectively inherit and simulate functional protein on the surface of a source cell membrane, systematically replace functions of the source cell in physiological and pathological processes, such as inflammatory factor adsorption, migration factor adsorption and the like, interfere and inhibit the disease process from the cellular mechanism, regulate and control cell composition and immune microenvironment of a focus part, and improve the immune function of the source cell membrane. The medicine does not need to be additionally carried. Aiming at the related pathogenesis of immune tolerance deficiency, the invention innovatively provides a therapeutic strategy for constructing immune tolerance. The homology and inherent immunosuppressive ability of tumor cells to the focus part of the autoimmune disease are reversely used, immune tolerance of the focus part based on immune checkpoints is reconstructed, meanwhile, target cells are replaced to adsorb autoimmune antibodies, and the immune response level is reduced.

The invention provides a VvYUC10 gene, the nucleotide sequence of the VvYUC10 gene is as shown in SEQ ID No.1, or the nucleotide sequence which is obtained by substituting, deleting and / or adding one or more nucleotides to the nucleotide sequence as shown in SEQ ID No.1 and expresses proteins with the same function; the invention also provides an application thereof. The grape VvYUC10 gene has the advantages that the function of the grape VvYUC10 gene is verified, it is found that the content of auxin in calluses of overexpressed VvYUC10 arabidopsis thaliana and grapes under salt stress is increased, the oxidation resistance is enhanced, and it is further found that the expression quantities of the VvRD22 gene, the VvDREB2A gene, the VvRD29A gene, the VvP5CS gene, the VvCAT1 gene and the VvSOD gene are increased; finally, it shows that the VvYUC10 gene positively regulates and controls the auxin signal channel to positively resist external adverse environmental factor salinization, and a theoretical reference basis is provided for grape salt-tolerant gene mining and breeding.

The invention discloses a metal-G4DNAzyme nucleic acid framework mediated enzyme immobilization method and an application of the metal-G4DNAzyme nucleic acid framework mediated enzyme immobilization method. The method comprises the following steps: co-incubating an enzyme and a G-quadruplex-containing nucleic acid sequence in a PBS buffer solution, adding divalent metal ions, placing in a centrifuge tube, oscillating, carrying out ultrasonic treatment, centrifuging a supernatant, washing with deionized water, redissolving and precipitating by adopting the PBS buffer solution, co-incubating with hyperchlorheme, centrifuging to remove the supernatant, and washing away redundant hyperchlorheme, thereby obtaining the G-quadruplex-containing nucleic acid sequence. And the metallo-G4DNAzyme nucleic acid framework mediated enzyme is obtained. The problems that an existing functional protein and nucleic acid coupling method is complex in process and harsh in condition, and activity and functions of protein are difficult to retain are solved. Coupling of enzyme and nucleic acid is achieved through biomimetic mineralization, and the formed functional metal nucleic acid framework immobilized enzyme has the dual functions of target recognition and signal amplification and is applied to detection of small molecular targets.

The invention belongs to the technical field of scar-free skin injury repair, and particularly relates to a magnetic particle-protein fiber dressing and a preparation method and application thereof. The preparation method comprises the following steps: coating magnetic nano-particles, mixing the obtained coated magnetic nano-particles with water-soluble copper salt and water to carry out copper ion loading, and mixing the obtained coated magnetic nano-particles loaded with copper ions with functional protein and a buffer solution to carry out incubation. And placing the obtained magnetic microsphere-protein compound solution in an external magnetic field for magnetic attraction, and then carrying out directional assembly in the external magnetic field to obtain the magnetic particle-protein fiber assembly. According to the magnetic particle-protein fiber assembly and the magnetic particle-protein fiber coating modified medical dressing provided by the invention, scar-free rapid repair of wounds can be realized, and the completeness of skin functions can be recovered; and the preparation method is simple and has important clinical application value and market prospect.

The invention discloses an application of a rape anthocyanin reductase BnaBAN gene or a protein coded by the BnaBAN gene in regulating and controlling the oil content of rape and the composition of unsaturated fatty acid. The nucleotide sequence of the BnaBAN gene is as shown in SEQ ID NO.1, or the sequence as shown in SEQ ID NO.1 is subjected to substitution and / or deletion and / or addition of one or more nucleotides and expresses a homologous gene of the same functional protein. An agrobacterium tumefaciens-mediated genetic transformation method is used for editing the BnaBAN gene, and it is found that BnaBAN mutation increases the oil content of rape seeds and affects fatty acid composition. The method disclosed by the invention has very important application prospects in high-oil-content breeding and oil quality improvement of the oilseed rape.