3results about How to "Increase transcription" patented technology

The application provides application of a CSRNP1 inhibitor in preparation of a medicine for treating liver ischemia-reperfusion injury. The application further clarifies a molecular mechanism in which CSRNP1 improves hepatocyte injury by targeting a MAPK signaling pathway mediated cell apoptosis in the process of ischemia-reperfusion, and provides a new treatment strategy for reducing ischemia-reperfusion injury in a liver surgery process, by deeply analyzing expression changes of CSRNP1 in the process of liver ischemia-reperfusion and correlation between the expression changes and ischemia-reperfusion injury caused by a liver surgery.

The application relates to the field of agricultural transgenic technology, and particularly discloses GhDBB28 application of a gene in regulating salt tolerance of upland cotton plants, the gene GhDBB28 The nucleotide sequence of the gene is shown as SEQ ID NO. 1; silencing the gene GhDBB28 increases the salt sensitivity of the upland cotton plants. The application reduces the activity of an antioxidant system in the upland cotton plants by silencing the gene GhDBB28 , so that the plants are more sensitive to salt stress, and a scientific basis is provided for constructing a transgenic upland cotton plant with new salt-sensitivity.

Provided are a nucleic acid, a vector and a host cell for preparing HBV cccDNA. The nucleic acid for preparing the HBV cccDNA comprises a first segment and a second segment connected to the first segment, wherein the first segment and the second segment are each independently transcribed; the first segment comprises a Core gene encoding a core protein, a Pol gene encoding the HBV polymerase, and an X gene encoding the HBx, and the second segment comprises a nucleic acid fragment corresponding to the pregenomic RNA and a nucleic acid fragment corresponding to the initiation codon; the nucleic acid fragment corresponding to the initiation codon is located at the 3' end in the transcription direction of the nucleic acid fragment corresponding to the pregenomic RNA; and in the second segment, there is only one nucleic acid fragment corresponding to the initiation codon, and the nucleic acid does not contain an expression cassette encoding the S gene of the HBs protein