5results about How to "Increase transcription" patented technology

The invention relates to the technical field of bioengineering, and discloses a marker for predicting liver cancer treatment sensitivity and application thereof.The marker for predicting liver cancer treatment sensitivity comprises HECTD2, the nucleotide sequence of the HECTD2 is shown as SEQ ID NO: 1, oxidative stress of the HECTD2 is limited through KEAP1 / NRF2 so as to promote drug resistance of the revatinib, the marker comprises H3K181a, H3K182a, H3K182a, H3K182a, H3K182a, H3K182a, H3K182a, H3K182a, H3K182a, H3K182a, H3K182a, H3K182a, H3K182a, H3K182a, H3K182a, H3K182a, H3K182a The nucleotide sequence of the H3K18la is as shown in SEQ ID NO: 2, and the H3K18la induces up-regulation of HECTD2 in HCC cells, so that the drug resistance of the lenvatinib is promoted. According to the present invention, the HECTD2 is adopted as the E3 ubiquitin ligase, and the K48-connected multi-ubiquitin chain is added on the K551 residue of the KEAP1 so as to alleviate the oxidative stress stimulated by the treatment of the lenvatinib; the transcription of the HECTD2 is enhanced by the H3K18la; the expression level of the HECTD2 is an independent prediction index of prognosis of the HCC patient; a new visual angle is provided for understanding the drug resistance of the HCC to the lenvatinib, and a promising diagnosis and treatment strategy is possibly provided for clinical treatment of the HCC.

The invention discloses a repair gene editing system aiming at HbE hematopoietic stem progenitor cell mutation site point mutation as well as a construction method and application thereof. The system comprises a nick RNA (Ribonucleic Acid) expression vector, an mRFP1 part, a peg expression vector plasmid and PEmax mRNA, wherein the nick RNA expression vector is formed by carrying out T4 connection on a vector skeleton obtained by carrying out enzyme digestion on pGL3-U6 plasmids and an oligo double-stranded DNA (Deoxyribose Nucleic Acid) sequence formed after annealing; the mRFP1 part is used for cutting off pU6-tevopreq1-GG-acceptor plasmids; the peg expression vector plasmid is used for inserting a redundant part into a designed peg-RNA sequence; the construction method comprises the following steps: firstly, determining a beta-thalassemia mutation site (beta CD26), then constructing a plasmid expression vector (a nick RNA expression vector is subjected to enzyme digestion, purification, connection design and other steps, and a peg expression vector is subjected to excision and sequence insertion), then transfecting a cell screening system by using lipo3000, determining the transfection efficiency, screening out sgRNA2 with high transfection rate, and using the sgRNA2 to prepare a kit. The method is used for accurately repairing Yunnan high-incidence beta-thalassemia mutation sites caused by point mutation of HbE hematopoietic stem / progenitor cell mutation sites.

The application provides application of a CSRNP1 inhibitor in preparation of a medicine for treating liver ischemia-reperfusion injury. The application further clarifies a molecular mechanism in which CSRNP1 improves hepatocyte injury by targeting a MAPK signaling pathway mediated cell apoptosis in the process of ischemia-reperfusion, and provides a new treatment strategy for reducing ischemia-reperfusion injury in a liver surgery process, by deeply analyzing expression changes of CSRNP1 in the process of liver ischemia-reperfusion and correlation between the expression changes and ischemia-reperfusion injury caused by a liver surgery.

The application relates to the field of agricultural transgenic technology, and particularly discloses GhDBB28 application of a gene in regulating salt tolerance of upland cotton plants, the gene GhDBB28 The nucleotide sequence of the gene is shown as SEQ ID NO. 1; silencing the gene GhDBB28 increases the salt sensitivity of the upland cotton plants. The application reduces the activity of an antioxidant system in the upland cotton plants by silencing the gene GhDBB28 , so that the plants are more sensitive to salt stress, and a scientific basis is provided for constructing a transgenic upland cotton plant with new salt-sensitivity.

Provided are a nucleic acid, a vector and a host cell for preparing HBV cccDNA. The nucleic acid for preparing the HBV cccDNA comprises a first segment and a second segment connected to the first segment, wherein the first segment and the second segment are each independently transcribed; the first segment comprises a Core gene encoding a core protein, a Pol gene encoding the HBV polymerase, and an X gene encoding the HBx, and the second segment comprises a nucleic acid fragment corresponding to the pregenomic RNA and a nucleic acid fragment corresponding to the initiation codon; the nucleic acid fragment corresponding to the initiation codon is located at the 3' end in the transcription direction of the nucleic acid fragment corresponding to the pregenomic RNA; and in the second segment, there is only one nucleic acid fragment corresponding to the initiation codon, and the nucleic acid does not contain an expression cassette encoding the S gene of the HBs protein