11559 results about "Botany" patented technology

Provided in the present invention are a creation method and apparatus for a tea leaf disease recognition model, and a device and a storage medium. The creation method comprises: acquiring a plurality of images of tea leaves, and disease and insect pest information corresponding to each image; generating a tea leaf disease recognition model, wherein the tea leaf disease recognition model includes a MobileNetV3 model, an ultra-lightweight attention model and a fully connected layer; and on the basis of the plurality of images of tea leaves and the disease and insect pest information corresponding to each image, training the tea leaf disease recognition model. The tea leaf disease recognition model can automatically recognize tea leaf diseases and insect pests.

The invention belongs to the technical field of cell cryopreservation, and relates to a cryopreservation method based on ultrasonic ice planting. In the method, the nucleation temperature of ice planting is less than-9 DEG C, and the cooling rate from nucleation to icing is less than 5 DEG C / min. Compared with the prior art, the method has the advantages that the time required for cryopreservation of the cells is remarkably shortened, meanwhile, damage to the cells caused by ice crystals generated in the cooling process is reduced, and the problem that an existing cell cryopreservation cooling method cannot meet the nucleation and freezing uniformity of a cell solution in the cooling process is solved.

The invention discloses application of TaMYB-7B and a coding gene thereof in regulation and control of wheat tiller number, and belongs to the technical field of gene engineering. The invention finds for the first time that the TaMYB-7B gene is a novel gene capable of simultaneously regulating and controlling the tillering number and grain traits of wheat. The TaMYB-7B gene is interfered, knocked out, silenced or mutated, so that the tiller number of a plant can be increased, the grain length is increased, the hundred-grain weight is increased, finally, the yield of a single wheat plant can be increased, and a foundation is laid for wheat yield improvement and character improvement.

The invention provides bacillus megaterium and application thereof in preparation of a nano-selenium organic fertilizer and improvement of the selenium content of tea leaves, and belongs to the technical field of microorganisms, the bacillus megaterium NT2 is preserved in the China Center for Type Culture Collection with the preservation number of CCTCC NO: M 20251806, and the bacillus megaterium NT2 is preserved in the China Center for Type Culture Collection with the preservation number of CCTCC NO: M 20251806. The bacillus megatherium NT2 has relatively strong resistance to reduction on selenium element, can survive in high-concentration inorganic selenium, can reduce the inorganic selenium into nano-selenium, and has relatively weak capability of enriching arsenic, cadmium and lead; the selenium source of the nano-selenium organic fertilizer can be prepared by mixed fermentation with high-selenium rock powder which is calcined and activated at high temperature, so that the content of heavy metals does not exceed the national standard while the selenium content of the tea leaves is increased. The bacillus megaterium provided by the invention not only can reduce the toxicity of inorganic selenium in high-selenium rock and convert the inorganic selenium into nano-selenium, but also can stably, safely and standardly produce selenium-rich tea leaves, the heavy metal content of the tea leaves does not exceed the standard, and the bacillus megaterium has a wide market prospect.

A crop loss monitoring method includes detecting crop loss in a measurement area when the measurement area corresponds to an unharvested area of a field and generating pre-harvest crop loss sensor data based thereon. The method further includes detecting crop loss in the measurement area when the measurement area is behind a distal end of a header of a harvester and generating harvesting crop loss sensor data based thereon. The method further includes identifying a pre-harvest crop loss value based on the pre-harvest crop loss sensor data, identifying a harvesting crop loss value based on the harvesting crop loss sensor data and the pre-harvest crop loss value, and generating one or more control signals based on at least one of the pre-harvest crop loss value or the harvesting crop loss value.

A crop loss monitoring method includes detecting crop loss in a measurement area, the measurement area corresponding to an unharvested area of a field. The method further includes identifying a pre-harvest crop loss value based on the crop loss detected in the measurement area. The method further includes generating one or more control signals based on the pre-harvesting crop loss value.

The application provides a wheat flour wet gluten content main effect site and SNP marker and application, and belongs to the wheat breeding technical field, and mainly provides SNP molecular markers Marker45889, Marker45896, Marker45348, Marker45403, Marker45397 and Marker45431, and the SNP site provided by the application has important significance for detecting and breeding wheat flour high FWG varieties, lines and breeding materials, and molecular markers can be developed according to the SNP site, so that the wheat quality breeding efficiency is accelerated.

The invention relates to the technical field of fertilizers, in particular to an environment-friendly water-soluble fertilizer containing a seaweed extract and polyglutamic acid. The water-soluble fertilizer is prepared by preparing seaweed oligosaccharide and double-grafted poly-L-gamma-sodium glutamate and compounding and complexing the seaweed oligosaccharide and the double-grafted poly-L-gamma-sodium glutamate, has the characteristics of low salting-out, high stability, no precipitation, good slow release performance and the like, and can continuously release nutrients and keep a solution clear and transparent under a low-salt condition; and because the polyglutamic acid and seaweed extract compound drip irrigation agent has the chelation and water retention characteristics of the polyglutamic acid and the natural growth promoting effect of the seaweed extract, the crop rhizosphere nutrient absorption efficiency and stress resistance are remarkably improved, the compound drip irrigation agent is suitable for drip irrigation and water and fertilizer integrated application of greenhouse vegetables, fruit trees and field crops, and the comprehensive effects of high efficiency, low salt load and environment friendliness are achieved.

Present invention teaches the method of using a keratin hydrolysis peptide (“KHP”) solution to improve the efficacy of fertilizer usage and absorption by soybeans. By selectively choosing specific weights of feathers and water, and treating the mixture to a high-temperature high-pressure hydrolysis process, the resulting solution is confirmed to contain at least 253 peptides and then infused to the fertilized soil in which the soybean seeds are grown, as well as the plants after the seed start sprouting. Optionally, the KHP solution can be diluted by water, as disclosed in the specification, for applying to the soil around the soybean.

The invention belongs to the technical field of plant genetic engineering molecular breeding, and particularly relates to application of wheat TabHLH93 protein or a coding gene thereof in regulation and control of plant grain development. According to the invention, a binary expression vector capable of realizing ectopic expression of the TabHLH93 gene is constructed and is genetically transformed into common wheat to obtain a progeny with high expression of the TabHLH93 gene, so that overexpression of the TabHLH93 gene is realized, and it is verified that the TabHLH93 has a function of regulating and controlling the size of wheat grains and also has an important regulation and control effect on agronomic characters such as wheat plant height, tiller number, spike length and thousand seed weight. A candidate gene with an important application prospect is provided for wheat high-yield genetic improvement, and the gene has important significance for guaranteeing grain safety.

The invention provides a humic acid type plant pollen sensitization inhibitor as well as a preparation method and a use method thereof, and the humic acid type plant pollen sensitization inhibitor is prepared from the following raw materials in parts by mass: 1 to 2 parts of polyvinyl alcohol, 0.01 to 0.05 part of guar gum, 2 to 10 parts of acrylic acid, 0.05 to 0.2 part of initiator, 0.5 to 2 parts of potassium humate and 50 to 250 parts of water. The humic acid type plant pollen allergy inhibitor is environment-friendly and low in cost, and has good biocompatibility and allergy inhibition effect.

The invention discloses a cotton water and fertilizer management method and system based on ground penetrating radar and unmanned aerial vehicle multispectral fusion integration, a terminal and a storage medium, and the method comprises the steps: obtaining the soil actual measurement water content, cotton leaf actual measurement nitrogen content, soil electromagnetic signals and cotton leaf spectral information of a target cotton field sample, and carrying out the preprocessing; obtaining soil water content inversion characteristics and cotton leaf nitrogen spectral characteristics; performing model training according to the actually measured soil water content and the soil water content inversion characteristics to obtain a target soil water content prediction model; performing model training according to the actually measured nitrogen content of the cotton leaves and the nitrogen spectral characteristics of the cotton leaves to obtain a target nitrogen prediction model; outputting the predicted soil water content and the predicted cotton leaf nitrogen content, and determining the irrigation amount and the nitrogen fertilization amount; and irrigating and fertilizing the target cotton field. According to the invention, the prediction efficiency and accuracy of the soil water content and the cotton leaf nitrogen content in the cotton field can be effectively improved, and precise irrigation and fertilization of the cotton field are realized.

The invention relates to the technical field of agricultural fertilizers, and particularly discloses a polypeptide fish protein flushing water-soluble fertilizer and a preparation method thereof, the water-soluble fertilizer is prepared from the following raw materials: unrotten small fish or fish sewer, edible cane sugar or brown granulated sugar, a self-prepared fish protein enzymolysis agent, a self-prepared fish protein anaerobic fermentation inoculant and water; the preparation method comprises an enzymolysis stage and a fermentation stage. The compound fertilizer can be used as a nitrogen fertilizer to replace a traditional chemical nitrogen fertilizer, or is matched with a trace element fertilizer in a compound fertilizer to be used in crop planting; the water-soluble fertilizer has the beneficial effects that the water-soluble fertilizer can supplement soil protein amino acid fertilizer nutrition and increase the content of nutritional ingredients in crops, fermented polypeptide micromolecule sugar can be directly absorbed by the crops to improve the taste, ecological organic planting is realized, meanwhile, the planting cost is reduced, and the water-soluble fertilizer is suitable for fruit and vegetable planting families, farms, picking gardens, greenhouse farmers and the like, is simple and easy to operate, and is suitable for popularization and application. Raw materials are easy to collect, low in cost and obvious in effect.

The invention belongs to the technical field of molecular biology, and particularly relates to a CeNF-YA3 gene, an expression vector and application of the CeNF-YA3 gene in vegetable fat regulation and control. The nucleotide sequence of the CeNF-YA3 gene is as shown in SEQ ID NO.1. After the CeNF-YA3 gene is over-expressed, the grease content of arabidopsis seeds and leaves can be remarkably increased; in addition, the invention also provides a series of vectors containing the CeNF-YA3 gene for subcellular localization, yeast hybridization and plant overexpression for the first time, and proves that the CeNF-YA3 protein has a transcriptional activation function, is localized in a cell nucleus and accords with the basic characteristics of transcription factors for the first time. Therefore, the technical scheme of the invention provides scientific guidance for the application of the CeNF-YA3 gene and the protein thereof.

The invention belongs to the technical field of research on the relationship between snow drought and vegetation browning, and particularly discloses a quantification method and terminal equipment for the transfer relationship between snow drought and vegetation browning, and the method comprises the steps: obtaining a standard rainfall index, a nonparametric standardized snow water equivalent index and a normalized vegetation index in a preset time period of a research region; the snow drought grade of the research area is determined according to the non-parametric standardized snow water equivalent index, the snow drought type of the research area is determined according to the standard rainfall index and the non-parametric standardized snow water equivalent index, and the snow drought type comprises dry snow drought and warm snow drought; according to the normalized vegetation index, the standard rainfall index and the nonparametric standardized snow water equivalent index, determining the transmission time of vegetation browning caused by the corresponding type of snow drought; and according to the transmission time, determining the transmission probability of vegetation browning of different degrees caused by different grades and different types of snow and drought. According to the method, the transmission time and the transmission probability from snow drought to vegetation browning are quantified, and early drought monitoring and early warning can be improved.

Disclosed herein are methods of increasing nitrogen fixation in a non-leguminous plant. The methods can comprise exposing the plant to a plurality of bacteria. Each member of the plurality comprises one or more genetic variations introduced into one or more genes or non-coding polynucleotides of the bacteria's nitrogen fixation or assimilation genetic regulatory network, such that the bacteria are capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen. The bacteria are not intergeneric microorganisms. Additionally, the bacteria, in planta, produce 1% or more of the fixed nitrogen in the plant.

The invention discloses a GmbZIP36 protein related to aluminum resistance of plants in acid soil as well as a coding gene and application of the GmbZIP36 protein, and belongs to the technical field of bioengineering. The amino acid sequence of the GmbZIP36 protein is shown as SEQ ID NO: 2, 3 or 4, and the nucleotide sequence of the coding gene of the GmbZIP36 protein is shown as SEQ ID NO: 1. The invention finds that the expression of the GmbZIP36 gene is induced by aluminum stress, the relative root elongation and root biomass of arabidopsis thaliana and soybean under the aluminum stress can be obviously improved by over-expressing the gene in a plant, and aluminum accumulation is reduced, so that the aluminum resistance of the plant is enhanced. The invention provides a recombinant vector containing the gene, a host cell and application of the recombinant vector and the host cell in regulating and controlling the aluminum resistance of plants. Meanwhile, the invention also provides a method for producing an aluminum-resistant transgenic plant by using the gene, a kit for detecting the gene or protein and a method for carrying out assisted breeding by using the molecular marker linked with the gene. The invention provides a key gene resource for aluminum-resistant molecular breeding of crops, and has important significance for cultivating new varieties of crops adapting to acid soil.

The invention discloses application of an apple MdMYS1 gene in regulating and controlling the wax content of plant fruits and leaves, and belongs to the technical field of plant genetic engineering. According to the invention, the MdMYS1 gene with high expression quantity in apple varieties with more waxiness is separated, and the nucleotide sequence of the MdMYS1 gene is shown as SEQ ID NO. 1. Through subcellular localization, the transcription factor expressed by the MdMYS1 gene is found to be localized on a cell nucleus. Experimental results show that overexpression of the MdMYS1 gene can significantly increase the wax content of the apple fruits and leaves by promoting biosynthesis of wax, which indicates that the MdMYS1 gene plays a key role in regulation and control of the wax content of the apple fruits and leaves. The invention provides an efficient and rapid way for apple breeding, provides a gene material for improving apple quality, and has a wide application prospect in improving economic benefits and ecological benefits of apple planting.

The invention discloses a preparation method and application of a mulberry leaf extract with high stability and high activity in a protein food system, and belongs to the field of food processing and nutrition and health. The mulberry leaf extract is prepared by taking mulberry leaves as a raw material through steam explosion and ball milling pretreatment, compound enzyme directional enzymolysis, ultrasonic-assisted extraction, protein-induced sedimentation combined with ultrafiltration membrane selective impurity removal, compound stabilization treatment and drying. The obtained extract is rich in 1-deoxynojirimycin (DNJ) and mulberry leaf polysaccharide, high-polymerization-degree polyphenol is remarkably removed, and high clarity and heat stability are achieved. The DNJ retention rate gt is obtained after the milk is stored at 37 DEG C for 7 days when the milk protein is applied to a milk system; and therefore, the product prepared by the method is obviously superior to the product prepared by the prior art. The extract is pure in flavor and high in biological activity, and can be widely applied to the field of healthy foods such as dairy products and protein beverages.

The present invention relates to tomato plants comprising two copies of an allele associated with enhanced resistance to ToBRFV infection. The invention also relates to seeds and parts of said plants, such as fruits. The invention further relates to methods of making, identifying and using such seeds and plants.

The invention relates to the technical field of agricultural microorganisms. The invention provides a compound microorganism bacterium suspension, a bacterium agent, a tobacco seed coating agent as well as a preparation method and application of the compound microorganism bacterium suspension. The compound microorganism bacterium suspension comprises a rice seed endophytic bacterium spiraceae R3 bacterium suspension and a rice seed endophytic bacterium stenotrophomonas R5 bacterium suspension; the mass ratio of R3 to R5 is 1: (0.5-1.5); the endophytic bacterium herbapiril R3 of the rice seeds is Herbapiril sp. R3, and the preservation number of the herbapiril R3 is CCTCC (China Center For Type Culture Collection) NO: M 20231757; the rice seed endophytic bacterium stenotrophomonas R5 is Shenotrophomonas sp. R5, and the preservation number of the rice seed endophytic bacterium stenotrophomonas R5 is CCTCC (China Center for Type Culture Collection) NO: M 20231758. The compound microbial suspension is used as a seed coating agent to coat tobacco seeds, and growth of tobacco root systems can be remarkably promoted.

The invention relates to the technical field of cheese, and provides a vegetable fat composition, vegetable cheese and a preparation method thereof, the vegetable fat composition comprises myristic acid and stearic acid in a mass ratio of (19-22): (2.5-4.5), and the melting point of the vegetable fat composition is 24.0-26.0 DEG C. The coconut oil is subjected to two-stage dry fractionation, coconut oil fractionation components obtained through separation are combined according to a certain proportion to form the coconut oil composition with specific fatty acid composition and a certain melting point range, and when the coconut oil composition is applied to preparation of the vegetable cheese, the taste problem of the existing vegetable cheese can be solved.

The invention provides a healthy plant community construction method based on an urban green land nutrition relationship, and relates to a construction method of an urban green land plant community. The construction method comprises the following steps: selecting plant communities to construct species according to urban green land types and functional requirements; according to the plant community establishment species, determining the nutrition relationship between the phytophagous arthropods corresponding to the establishment species plants and the nutrition relationship between the phytophagous arthropods corresponding to the natural enemies; and selecting functional plants according to the corresponding nutrition relationship, and configuring the functional plants around the colonized plants to construct and form a healthy plant community based on the urban green land nutrition relationship. According to the construction method, healthy growth of plants in the urban green land, nutritional relation coupling and ecological system balance of the urban green land can be realized.

The invention belongs to the technical field of molecular biology, and particularly relates to a CeNF-YA1 gene, an expression vector and application of the CeNF-YA1 gene in vegetable fat regulation and control. The invention provides the CeNF-YA1 gene for the first time, the nucleotide sequence of the CeNF-YA1 gene is as shown in SEQ ID NO.1, the full length of a coding region of the CeNF-YA1 gene is 792 bp, a series of carriers containing the CeNF-YA1 gene, such as subcellular localization, yeast hybridization and plant overexpression, are constructed for the first time, and meanwhile, the CeNF-YA1 protein is proved to have a transcription activation function, is localized in a cell nucleus and conforms to the basic characteristics of transcription factors. It is found for the first time that overexpression of the CeNF-YA1 gene can significantly improve the grease content of arabidopsis seeds and leaves; therefore, the technical scheme provided by the invention can be used for improving the oil content of the plant seeds and the nutritional tissues, and shows the application prospect in improving the oil content of the seeds and the nutritional tissues.

The invention relates to the field of polypeptides, in particular to a red camellia polypeptide as well as a preparation method and application thereof. Red camellia is used as a raw material, high-pressure homogenization wall breaking and pepsase enzymolysis are performed, and separation and purification are performed to obtain the red camellia polypeptide with repairing, moisturizing, oxidation resisting and nourishing functions. The polypeptide prepared by the invention is more efficient, safer and more environment-friendly, and has a wide application prospect in the fields of cosmetics, functional foods and biological medicines.

The invention provides a wormwood planting fertilizer and application thereof, and belongs to the technical field of agricultural planting. The wormwood planting fertilizer is prepared from the following components in parts by weight: 10 to 15 parts of humic acid, 5 to 8 parts of amino acid, 2 to 3 parts of wormwood extract A, 3 to 5 parts of wormwood extract B, 15 to 20 parts of nitrogen-phosphorus-potassium compound fertilizer, 0.01 to 0.03 part of sodium selenite, 0.02 to 0.05 part of zinc sulfate, 0.3 to 0.5 part of cellulose nanocrystal, 0.01 to 0.03 part of malic acid and 0.1 to 0.2 part of sodium alginate. By means of the two-dimensional design that components synergistically enhance absorption and the planting process is matched with the growth rhythm, the problems that in traditional fertilization, nutrients are unbalanced, the selenium and zinc utilization rate is low, and pest and disease damage prevention and control are difficult are solved, efficient absorption of wormwood on microelements, accurate supply of nutrients and reduction of the pesticide use amount are achieved, and the yield of the wormwood is increased. The yield and the medicinal quality are synergistically improved.

The invention discloses a novel long-acting bird repellent and a preparation method thereof, and belongs to the technical field of chemical bird repelling. The bird repellent is composed of a core material and a wall material, the core material is a mixture of geraniol, cinnamyl aldehyde and allicin according to the mass ratio of 1: (1.2-1.5): (0.5-0.8), the wall material is modified beta-cyclodextrin and pumpkin seed protein nanoparticles, and the mass ratio of the geraniol, the cinnamyl aldehyde and the allicin is (5-7): (3-5): (1-3). The preparation method comprises the following steps: preparing the quaternary ammonium salt modified beta-cyclodextrin, including the core material, performing high-speed shearing emulsification on the quaternary ammonium salt modified beta-cyclodextrin and the pumpkin seed protein nano dispersion liquid, and performing freeze drying to obtain a final product. According to the invention, a stable and compact'molecular inclusion + physical barrier 'dual slow-release structure is constructed by utilizing the electrostatic interaction between the positively charged modified beta-cyclodextrin and the negatively charged protein nanoparticles, the release time of effective components is remarkably prolonged to 9 months or more, and the composite material has excellent rain wash resistance and ultraviolet resistance, and can be used for preparing a composite material with a good application prospect. And efficient, environment-friendly and long-acting bird repelling is realized.

The invention belongs to the technical field of molecular biology and genetic breeding, and discloses a molecular marker closely linked with soybean plant height QTL qPH1.01 and application of the molecular marker. According to the present invention, the major QTL qPH1.01 for regulating corn plant height variation is identified by using linkage analysis, the significantly associated SNP is located at the 27th, 318th and 904th bases of the first chromosome of the reference genome Glycinemaxv2.1, 6.28-11.14% phenotypic variation can be explained in the single environment, the PARMS marker developed by using the SNP is used to detect 96 soybean varieties, the typing is clear, the operation is simple and convenient, the method is suitable for soybean plant polymer breeding, and the application prospect is broad. And a new high-yield soybean variety with proper plant height and lodging resistance is cultivated.

The utility model discloses plant extract extraction equipment, which belongs to the technical field of plant extraction and comprises an extraction barrel, a supporting plate is fixedly mounted on the upper surface of the extraction barrel, a fixing plate is fixedly mounted on the upper surface of the supporting plate, and a pressing component and a lifting component are arranged on the upper surface of the fixing plate; according to the device, the pressing assembly is arranged, the other toothed chain wheel drives the reciprocating lead screw to rotate under the action of the toothed chain, at the moment, the lower sliding sleeve can drive the pressing plate to move up and down in the filter barrel under the limitation of the air cylinder under the action of the thread, plants in the filter barrel can be extruded when the pressing plate moves downwards, and when the pressing plate moves upwards, the plants can be extruded by the reciprocating lead screw. When the plant is not extruded, the solvent can be absorbed, so that the plant is filled with the solvent, the plant is pressed while rotating in the plant soaking process, the full contact between the plant and the solvent is further ensured, the extraction quality of plant extraction is improved, the solvent waste can be avoided by pressing during discharging, and the purpose of saving resources is achieved.