33921 results about "Botany" patented technology

Provided herein are compositions and methods to improve plant resistance to abiotic and biotic stress, thereby improving crop yield. Provided herein are compositions including zinc, copper. Compositions described herein have plant priming activity where the plant's defenses against abiotic and biotic stress are boosted.

Disclosed herein are methods, compositions, and kits for determining the presence or absence of a target polypeptide (e.g., a polypeptide involved in nitrogen fixation) in a sample (e.g., a sample of soil from the rhizosphere of a non-leguminous plant).

The invention relates to the field of soil spectrum analysis, in particular to a farmland soil environment detection method and system. The method comprises the following steps: firstly, analyzing fluctuation characteristics of near infrared spectrum curves of a plurality of soil samples in a preset window of a target wavelength to obtain undisturbed degrees of the near infrared spectrum curves in the target wavelength; fitting spectral data points of the near infrared spectral curve, and obtaining feature retention degrees of the extreme points on the fitted curve according to the difference of fitting absorbance of adjacent extreme points on the fitted curve, the absorbance of each wavelength and the difference of the fitting absorbance and the wavelength distribution of the extreme points on each fitted curve; filtering the spectral data points of the target curve based on the non-interference degree and the feature retention degree to obtain a filtering curve; according to the method, the interference information in the near infrared spectrum curve is removed, the existing key information is reserved as much as possible, and the precision of farmland soil environment detection is improved.

The invention discloses a bacillus subtilis microbial agent for promoting decay of corn straws and application of the bacillus subtilis microbial agent, the preservation date of bacillus subtilis K15 is May 21, 2024, the preservation unit is China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.30716. The bacillus subtilis K15 has the advantages that the preservation number is CGMCC No.30716; the bacillus subtilis K15 is screened from soil in North China Plain, tests prove that the strain produces carboxymethyl cellulase, decomposition of corn straw can be effectively promoted, the decomposed straw provides a fertilizer source for wheat growth, growth of wheat plants and root systems is promoted, and the wheat yield is effectively increased. According to the invention, the function of the bacillus subtilis in straw decay promotion under the winter low-temperature condition in North China Plain is excavated, and a new strain resource is provided for a microbial fertilizer.

Provided in the present invention are a creation method and apparatus for a tea leaf disease recognition model, and a device and a storage medium. The creation method comprises: acquiring a plurality of images of tea leaves, and disease and insect pest information corresponding to each image; generating a tea leaf disease recognition model, wherein the tea leaf disease recognition model includes a MobileNetV3 model, an ultra-lightweight attention model and a fully connected layer; and on the basis of the plurality of images of tea leaves and the disease and insect pest information corresponding to each image, training the tea leaf disease recognition model. The tea leaf disease recognition model can automatically recognize tea leaf diseases and insect pests.

The invention discloses a wheat salt-tolerant gene and application thereof. Specifically, the invention discloses a protein TaSGR (SEQ ID NO: 3, 6 and / or 9) and an application of a coding gene of the protein TaSGR in regulation and control of plant salt tolerance and / or plant height. According to the invention, a homozygous gene editing plant of which the TaSGR gene is knocked out is successfully created by utilizing a CRISPR / Cas9 technology. The result shows that after the TaSGR gene is knocked out, the plant height of the wheat is obviously reduced, and the salt tolerance is obviously enhanced. The invention discloses the application of the protein TaSGR and the coding gene thereof for the first time. The salt tolerance of the plant is increased and / or the plant height of the plant is reduced by reducing the content and / or activity of the protein TaSGR (for example, the TaSGR gene is knocked out or silenced). According to the invention, a good gene resource is provided for salt-tolerant breeding of wheat, a new field is developed for application of the TaSGR gene, and the TaSGR gene has important significance for cultivation of novel salt-tolerant wheat.

The invention discloses a soilless culture nutrient solution containing a solid hydrogen supply material and an irrigation method and system. Raw materials of the soilless culture nutrient solution comprise the solid hydrogen supply material, a nutrient solution and a pH regulator. The use amount of the solid hydrogen supply material is 0.05-5 mmol / L; the dosage of the pH regulator is 0.01 to 50 mmol; all the raw materials are mixed, and the soilless culture nutrient solution with the irrigation hydrogen concentration larger than or equal to 500 ppb / L and the pH value of 5-8 is provided. On the basis of applying the soilless culture nutrient solution, the pH value and the hydrogen concentration of the soilless culture nutrient solution are monitored in real time, and the pH value and the hydrogen concentration of the soilless culture nutrient solution are adjusted and controlled by adjusting and controlling at least one of the applying amount of the soilless culture nutrient solution, the applying speed of the soilless culture nutrient solution, the using amount of a solid hydrogen supply material and the using amount of a pH adjusting agent. The hydrogen-rich nutrient solution can be controllably provided for soilless culture plants, stable release of hydrogen and proper adjustment of the pH value and nutritional ingredients are achieved, and the requirements of different crops and different growth stages are met.

The present disclosure relates to the field of biotechnology and provides novel recombinant DNA molecules for conferring tolerance to icafolin herbicides. The present disclosure also provides herbicide tolerant transgenic plants, seeds, cells, and plant parts comprising the recombinant DNA molecules, and methods of using the same.

The invention relates to bacillus velezensis and application thereof in preparation of a water-soluble microbial agent, and belongs to the technical field of microorganisms. The bacillus velezensis has the effects of producing exopolysaccharides, promoting the formation of soil aggregates, degrading abamectin, hydrolyzing protein, producing indoleacetic acid, promoting the growth of tomato plants and promoting the enzyme activity of tomato rhizosphere soil enzyme. The fertilizer not only has the effects of improving the soil aggregate structure and promoting the growth of tomato plants, but also can accelerate the temperature rise of the abamectin fermentation waste residue compost, prolong the high-temperature period and shorten the compost period, and is beneficial to promoting the harmless treatment and resource utilization of the abamectin fermentation waste residue.

Herbicidally active 3-phenylisoxazoline-5-carboxamides of cyclopentenylcarboxylic acid derivativesThe invention relates to 3-phenylisoxazoline-5-carboxamides of cyclopentenylcarboxylic acid derivatives of the general formula (I) and their agrochemically acceptable saltsand to their use in the crop protection sector.

The invention relates to bacillus velezensis and application thereof in preparation of a multifunctional straw decomposition agent, and belongs to the technical field of microorganisms. The bacillus velezensis has the functions of producing cellulase, producing xylanase, degrading abamectin, decomposing inorganic phosphorus and degrading straws; when straw is returned to the field, the multifunctional straw decomposition agent provided by the invention not only can accelerate the decomposition speed of straw, decompose abamectin residues in soil and improve the soil ecology, but also can promote the growth of pepper plants and enhance the stress resistance of the pepper plants, and has a good comprehensive application effect.

The invention provides a Chinese rose heat shock protein family gene RcHSP70-8, the nucleotide sequence of the Chinese rose heat shock protein family gene RcHSP70-8 is shown as SEQ ID No. 1, and the amino acid sequence of the Chinese rose heat shock protein family gene RcHSP70-8 is shown as SEQ ID No. 2. The invention discloses an application of the Chinese rose heat shock protein family gene RcHSP70-8 in delaying flowering of plants, and the RcHSP70-8 protein is positioned in a cell nucleus and can delay flowering through overexpression in arabidopsis thaliana. The invention is helpful for enriching the research on the flowering regulation and control mechanism of the Chinese rose, and has important significance for genetic engineering breeding.

The invention discloses a corn germination image segmentation method based on an elite adaptive rime algorithm. Relates to the technical field of agricultural seed detection and image processing, in particular to the technical field of corn germination image segmentation based on an elite adaptive rime algorithm. According to the method, a dual-adaptive weight mechanism and an elite reselection strategy are introduced into a rime optimization algorithm, the convergence capability of the algorithm is enhanced, and multi-threshold segmentation is carried out on the corn kernel germination image in combination with the Kapur entropy. And the image segmentation precision is effectively improved. The method comprises the following steps: acquiring a corn germination image data set; drawing a two-dimensional histogram, and inputting the two-dimensional histogram into a Kapur entropy function to obtain an objective function fobj; an elite solution module is initialized; a dual-adaptive weight mechanism is added; a soft rime strategy and a hard rime strategy are improved; updating the elite solution after the rime search strategy module; and the target function fobj is input into a rime improvement algorithm, and an optimal threshold value is obtained.

A wheat variety designated KS12D03-1 is disclosed, including plants and seeds thereof. KS12D03-1 is a durum wheat variety with a winter growth habit making it suitable for production in western Kansas and the High Plains. Methods for producing a wheat plant produced by crossing the variety KS12D03-1 with another wheat plant, and hybrid wheat seeds and plants produced by crossing the variety KS12D03-1 with another wheat line or plant, and the creation of variants by backcrossing, mutagenesis or transformation of variety KS12D03-1 are disclosed. Methods for producing other wheat varieties or breeding lines derived from wheat variety KS12D03-1 and to wheat varieties or breeding lines produced by those methods are also provided.

The invention relates to the field of computer vision and agricultural automation, and discloses a grape cluster young fruit lightweight detection method based on improved YOLOv8n, and the method comprises the following steps: obtaining grape cluster young fruit image data, and generating a grape cluster young fruit data set; improvement is carried out based on a YOLOv8n model, and the method comprises the steps that a C2f-Faster module is adopted to replace a C2f module to reduce the floating point calculation amount and the number of parameters, depth separable convolution is used to reduce the complexity of the model, a context anchor attention module is introduced to enhance the extraction ability of remote context information, and a Wi-I oU loss function is adopted to improve the generalization ability of the model. Training and optimizing the improved model; and carrying out real-time detection on young fruits of the grape cluster by utilizing the trained model. According to the method, the detection precision of the young fruits of the grape clusters is effectively improved, the model complexity is reduced, efficient and lightweight real-time detection is realized, and the method is suitable for an automatic grape fruit thinning system.

The invention provides a black soil cultivated land organic carbon degradation diagnosis method based on geographic big data and AI, and the method comprises the steps: constructing a black soil area cultivated land organic carbon degradation potential influence factor data set and a diagnosis model, judging the spatial correlation of potential influence factors, training the diagnosis model based on the data set, and obtaining a diagnosis result of the black soil area cultivated land organic carbon degradation. And performing black soil area cultivated land organic carbon degradation influence factor identification based on the trained diagnosis model, and performing organic carbon degradation influence factor action mechanism analysis based on an identification result. According to the method, by utilizing high resolution and high timeliness of geographic space big data and cloud computing, multiple types of potential factors such as climate, soil, terrain and agricultural activities which influence organic carbon change of the cultivated land in the black soil area can be quickly calculated on a large-area scale, and manpower, material resources and time costs caused by large-area data collection and analysis are avoided; by using an artificial intelligence algorithm, the main control factor of organic carbon degradation of the cultivated land in the black soil area is accurately identified from multiple potential factors, and the current degradation situation can be objectively evaluated.

The invention discloses a sea cucumber protein heptapeptide with functions of reducing blood pressure and blood sugar and application of the sea cucumber protein heptapeptide, and belongs to the technical field of bioactive peptide biology. The amino acid sequence of the sea cucumber protein heptapeptide is LPPGPFP. The sea cucumber protein heptapeptide can significantly inhibit the activity of ACE through a competitive inhibition mode, tolerates the gastrointestinal tract digestion environment, also has DPP-IV inhibition activity, can relieve hypertension and type 2 diabetes mellitus at the same time, and has an application value in preparation of products with the efficacy of reducing blood pressure and blood sugar due to multiple functions of the sea cucumber protein heptapeptide. The sea cucumber protein heptapeptide provided by the invention can significantly inhibit the activity of ACE through a competitive inhibition mode, has DPP-IV inhibition activity, has the characteristics of simple structure, safety, strong activity and the like, and has a wide application prospect.

The invention relates to the field of gene editing, in particular to a peony PoLEC1 gene and application of protein coded by the peony PoLEC1 gene. The peony PoLEC1 gene is cloned from cultivated peony paeonia ostii, after the peony PoLEC1 gene is transformed into arabidopsis thaliana, phenotypic contrast finds that the peony PoLEC1 gene can play a role in bolting and flowering as well as growth and development of plants, specifically, overexpression of the peony PoLEC1 gene can increase the number of leaves, delay bolting and flowering time and reduce the height of the plants. According to the invention, arabidopsis thaliana is taken as a model plant, and a certain theoretical basis is provided for establishing a complete plant regeneration and genetic transformation system for peony by exploring the functional characteristics of the peony PoLEC1 gene and the expression characteristics of the peony PoLEC1 gene in the whole somatic embryo direct generation process and in each tissue of overexpressed arabidopsis thaliana.

The invention provides application of a transcription factor CsPIF7 gene in regulation and control of theanine synthesis and improvement of tea quality, and belongs to the technical field of agricultural molecular biology. The invention finds that high expression of CsPIF7 can activate expression of TS1 and GS1, so that more theanine can be accumulated in tea leaves; after the expression of CsPIF7 is reduced by a tea tree VIGS technology, the activation and expression of TS1 and GS1 can be reduced, so that the content of theanine in tea leaves is reduced. Furthermore, the tea tree transcription factor CsPIF7 is found to be capable of positively regulating and controlling expression of TS1 and GS1 by utilizing LUC verification, and can be used for regulating and controlling the content of theanine in tea trees and improving the quality of tea leaves.

The invention provides a tea tree fresh leaf identification method and system based on YOLOv8 in the technical field of tea leaf detection, and the method comprises the following steps: S1, obtaining a large number of historical images of tea tree fresh leaves, carrying out the preprocessing of data annotation and data expansion of each historical image, and constructing a data set; s2, creating a tea tree fresh leaf recognition model based on a YOLOv8 network, and setting a loss function of the tea tree fresh leaf recognition model as an I nner-S I oU function; s3, dividing the data set into a training set, a verification set and a test set based on a preset proportion, and training, verifying and testing the tea tree fresh leaf recognition model through the training set, the verification set and the test set; and S4, deploying the tea tree fresh leaf recognition model passing the test, and performing tea tree fresh leaf recognition by using the deployed tea tree fresh leaf recognition model. The method has the advantage that the identification accuracy of the fresh tea leaves is greatly improved.

An underwater in-situ sea cucumber volume measurement method based on binocular vision and electronic equipment belong to the field of image processing, are used for solving the problem of keeping in-situ sea cucumber volume measurement, and are technically characterized in that a sea cucumber image is detected in underwater images collected by a binocular camera to obtain a sea cucumber image detection frame; segmenting a sea cucumber image detected in the image according to the sea cucumber image detection frame, and calculating mask information of the sea cucumber on an image plane; mapping the mask information to a global point cloud constructed by binocular information, and obtaining point cloud data of the sea cucumber; performing grid reconstruction on the point cloud data of the sea cucumber to obtain a continuous triangular grid; according to the method, the continuous triangular grids are converted into voxel representation, the number of non-zero elements in the voxel grids and the volume of each element are counted, the volume of the grids is estimated, and the volume of the sea cucumbers is obtained, so that the volume of the in-situ sea cucumbers can be accurately evaluated, and more accurate data support is provided for scientific cultivation of the sea cucumbers.

The invention discloses talaromyces purpurogenus, a preparation method and application in prevention and treatment of corn stalk rot, and provides a novel strain, namely talaromyces purpurogenus Jnf41, greenhouse control effect experiments show that the talaromyces purpurogenus Jnf41 can inhibit the scab area and the scab length of the corn stalk rot in greenhouse control effect determination, and the talaromyces purpurogenus Jnf41 can be used for preventing and treating the corn stalk rot. Infection of the corn stalk rot can be effectively inhibited, pathogenic bacteria are not prone to generating drug resistance, green prevention and control are implemented in the prevention and control process, and the biological preparation for resisting the corn stalk rot is prepared and has important significance on green prevention and control of the corn stalk rot.

Provided are compositions for genome editing and site-directed integration in plants comprising microprojectile particles coated, treated of applied with a recombinant DNA construct comprising a sequence encoding one or more genome editing reagents for delivery to a mature embryo explant from dry seeds. Further provided are methods for genome editing and site-directed integration in at least one cell of a plant using the disclosed compositions, and plants, plant parts and seeds comprising an edited genome or site-directed integration, which are produced by the disclosed methods.