45 results about "Zinc finger" patented technology

The present disclosure relates to dihydropyrimidine-2,4(1H,3H)-dione-containing polycyclic compounds, or pharmaceutically acceptable salts thereof, and compositions thereof which are effective to reduce or modulate widely interspaced zinc finger motifs (WIZ) protein expression levels and / or induce fetal hemoglobin (HbF) expression, and also relates to methods of their preparation and their use in treatment of subjects in need.

The invention discloses a brassica plant drought resistance negative regulation factor BnaA9.TZF5 and application of the brassica plant drought resistance negative regulation factor BnaA9.TZF5. The BnaA9.TZF5 is a transcription factor gene for coding CCCH type zinc finger protein in brassica napus, and a knockout plant obtained by performing targeted editing on the BnaA9.TZF5 through CRISPR / Cas9 shows that the drought resistance is remarkably enhanced along with phenotypic changes such as increase of leaf epidermis wax deposition and reduction of water loss rate; and an overexpression plant of the gene shows that the wax is reduced, the water loss is accelerated and the drought resistance is weakened, so that the BnaA9.TZF5 is a negative regulation factor for the drought resistance of the brassica plant. The BnaA9.TZF5 gene expression is knocked out to improve the drought resistance of brassica plants, the BnaA9.TZF5 gene can be used for molecular breeding of crops such as brassica napus, Chinese cabbage, cabbage and mustard, and a novel drought-resistant improved target gene is provided.

Methods and compositions for treating a corneal wound and / or increasing a population of limbal epithelial stem cells (LESCs) and / or early transit amplifying cells (eTAs) in the limbal epithelium of a subject in need thereof. The methods can include administering a therapeutic agent to the subject that: i) results in an increase in concentration of IFITM1 (Interferon Induced transmembrane Protein 1) in the limbal epithelium of the subject; and / or ii) results in a decrease in concentration of OVOL1 (Ovo Like Zinc Finger 1) in the limbal epithelium of the subject. Also provided are adeno-associated viruses (AAVs). The AAVs comprise a heterologous nucleic acid sequence encoding IFITM1 or a variant thereof, operably linked to a regulatory sequence.

The application discloses application of a CCHC type zinc finger protein PbrZFP719 gene in promoting pear pollen tube growth and belongs to the technical field of plant genetic engineering. The application clones the gene PbrZFP719 from 'yellow flower pear' pollen by using a plant gene cloning technique, the nucleotide sequence of the gene is shown in SEQ ID No. 1, and the amino acid sequence coded by the gene is shown in the sequence table SEQ ID No. 2. Research shows that the PbrZFP719 gene can promote pear pollen tube growth, the change of PbrZFP719 gene expression is related to the change of ROS level and cellulose content at the top of the pollen tube, and meanwhile, the PbrZFP719 gene also participates in the regulation mechanism of self-incompatibility reaction. The pollen magnetic transfection technology is used to study the function of the gene in the pollen tube, and provides a wide application prospect for improving pollination efficiency.

The invention discloses application of a tomato gene SlZFP2 as a negative regulation factor in regulation of plant cold resistance, belongs to the technical field of plant genetic engineering, and finds and confirms that the tomato zinc finger protein gene SlZFP2 is a key negative regulation factor of plant cold resistance for the first time. Experiments show that the expression of the SlZFP2 is induced by low temperature, the overexpressed transgenic tomato plant shows more serious cold injury symptoms under low temperature stress, the photosynthetic efficiency is reduced, active oxygen (H2O2 and O2-) is excessively accumulated, and the activity of antioxidant enzymes (SOD, POD and CAT) is weakened; the overexpression of the SlZFP2 can inhibit the biosynthesis and signal transduction of abscisic acid (ABA), and down-regulate the expression of cold response genes SlGRAS4 and SlCBF1; based on the discovery, the invention provides an application of using the SlZFP2 gene or an expression product thereof as a molecular marker for screening tomato materials with different cold resistance, creating a transgenic plant with reduced cold resistance by overexpressing the SlZFP2, and providing a recombinant DNA construct containing an SlZFP2 coding sequence and a host cell.

Disclosed are polypeptides useful in the treatment of diseases associated with pathogenic genomic repetitive sequences, such as neurological diseases. Nucleic acid molecules and vectors encoding such polypeptides are also disclosed. Also disclosed are therapeutic uses and methods of treating such diseases; in particular, the present invention relates to therapeutic uses and methods for the treatment of Friedel's Ataxia (FRDA). Also disclosed are methods and related peptides and nucleic acids for active, long-term delivery of therapeutic molecules to target cells in vivo or in vitro.

Disclosed are compounds and salts thereof that bind to and modulate the activity of cereblon. In some embodiments, the binding and modulation of cereblon results in the degradation of IKAROS family zinc finger proteins (e.g., IKZF2). The compounds are represented by Formula I: TIFF2026502995000081.tif43170

The present disclosure provides methods and compositions for inactivating a glutamine synthetase (GS) gene. Zinc finger DNA-binding domains engineered to bind in a GS gene and fusion proteins comprising a zinc finger DNA-binding domain and a cleavage domain are provided herein. Methods of using the zinc finger proteins and fusions thereof to inactivate GS in a cell or cell line are also provided.

The invention discloses an application of corn E3 ubiquitinase in enhancing the resistance of corn to corn stem rot and drought. The name of the corn E3 ubiquitinating enzyme is ZmWIPF2, and the corn E3 ubiquitinating enzyme is shown as a sequence 2 in a sequence table. When the corn is subjected to abiotic stress such as drought and biological stress such as stem rot, the ZmWIPF2 cooperates with related genes to regulate and control the corn to resist the stem rot and drought and respond to pathogenic bacterium infection and drought stress. The ZmWIPF2 gene contains a zinc finger structural domain and an RING-H2 structural domain, wherein RING-Ubox is an E3 ubiquitin ligase. Transgenic material verification proves that the gene can improve the stress resistance of corn, has important significance for breeding stress-resistant corn varieties, and is suitable for wide popularization.

Use of a Glycine soja zinc finger protein 4 (GsZFP4) or a genetic material thereof are provided. A gene GsZFP4 encoding the GsZFP4 has the nucleotide sequence of SEQ ID NO: 1. A constructed plant overexpression vector pBA002-GsZFP4 is transformed into soybean. After 21 d of treatment with an SMV strain SC7, it is found that disease symptoms in the soybean overexpressing GsZFP4 are significantly alleviated and the SMV content is significantly reduced, demonstrating resistance to the SMV. In summary, the GsZFP4 may positively regulate soybean resistance to the SMV.

The invention is applicable to the technical field of animal model construction, and provides a method for constructing a rabbit growth trait model based on ZDHHC23 gene knockout, and the method comprises the following steps: transcription synthesis of SpG-Cas9 mRNA; the method comprises the following steps: designing and synthesizing a zinc finger structural domain sgRNA of a rabbit ZDHHC23, namely designing an action target sgRNA on a rabbit ZDHHC23 gene aiming at the zinc finger structural domain of the rabbit ZDHHC23; and preparing the ZDHHC23 gene edited rabbit by an embryo microinjection technology. According to the embodiment of the invention, a SpG-Cas9 gene editing technology is utilized, gene knockout is carried out on the zinc finger structural domain of the rabbit ZDHHC23, a new variety of the rabbit with increased weight is obtained, and the method has application value in promoting improvement of genetic characters of the rabbit and improving production performance and economic value of the rabbit.

The invention belongs to the technical field of plant molecular biology, and relates to application of a gene CmZFP for regulating and controlling sucrose accumulation of muskmelon fruits. The CmZFP gene provided by the invention encodes a melon zinc finger protein transcription factor, can significantly improve the accumulation of sucrose in melon fruits by promoting the expression of a key gene CmNAG2 in a glycometabolism pathway, and plays an important role in the glycometabolism pathway. The invention provides a new gene resource for genetic improvement of the sugar character of the muskmelon, and can be used for cultivating a new variety of the high-sugar muskmelon and improving the fruit quality and the economic benefit.

The invention discloses a human zinc finger-truncated nucleus receptor fused small molecule response type gene regulation system and application thereof, and relates to the technical field of gene engineering. The gene regulation and control system contains transcriptional regulation and control protein, and the transcriptional regulation and control protein is formed by fusing a human zinc finger DNA binding module and a truncated nuclear receptor; the human zinc finger DNA binding module can specifically recognize and bind a target DNA regulatory sequence, and the truncated nuclear receptor is combined with a ligand to serve as a transcriptional regulatory module to realize controllable expression of a target gene; the human zinc finger DNA binding module is a human or humanized zinc finger array; according to the truncated nuclear receptor, an inherent DNA binding domain and an N-terminal transcriptional activation region are removed, and a hinge region and a ligand binding region are mainly reserved. The gene regulation system disclosed by the invention has the characteristics of humanization, compact structure, capability of being regulated by small molecules and the like, and in some embodiments, in-vivo or in-vitro controllable expression of a target gene can be realized.

This disclosure relates to dihydropyrimidine-2,4(1H,3H)-dione-containing polycyclic compounds or pharmaceutically acceptable salts thereof and compositions thereof that effectively reduce or regulate the expression level of broad-space zinc finger motif (WIZ) proteins and / or induce the expression of fetal hemoglobin (HbF), as well as methods for preparing them and their use in the treatment of subjects requiring them.

The present disclosure provides zinc finger fusion proteins that inhibit the expression of Nav1.7 in the nervous system, and methods of using the proteins in the treatment of pain disorders.

The application discloses a Pinus massoniana tandem zinc finger structure PmTZF1 gene and an expression protein and application thereof, and relates to the technical field of plant genetic engineering.The Pinus massoniana tandem zinc finger structure PmTZF1 gene is disclosed for the first time, a nucleotide sequence of which is shown as SEQ ID NO.1, and an amino acid sequence of which is shown as SEQ ID NO.2.The application constructs an expression vector pBI121-PmTZF1 of the Pinus massoniana tandem zinc finger structure PmTZF1 gene; the constructed expression vector of the Pinus massoniana tandem zinc finger structure PmTZF1 gene is transformed into Arabidopsis; and a transgenic Arabidopsis plant with improved non-biological stress resistance is obtained through cultivation and screening.Compared with a wild type plant, the root of the transgenic strain is significantly longer than that of the wild type, and the transgenic strain blooms and sprouts earlier; under drought stress, the transgenic strain normally blooms and bears fruits, has more branches, and has more silique quantity, length and other properties, which are significantly better than those of the wild type strain.

Provided is a retrotransposase which comprises a target DNA binding domain comprising a zinc finger binding motif, a reverse transcriptase domain, and an endonuclease domain, and which is capable of reverse transcription of RNA to DNA. The invention also relates to a system for modifying DNA. The system comprises the retrotransposase or a nucleic acid encoding the retrotransposase; and a donor RNA or a nucleic acid encoding the donor RNA, the donor RNA comprising: a sequence that binds to the retrotransposase and a heterologous sequence.

The application discloses a DNA binding single-zinc finger protein OsDOF22 The application discloses a DNA binding single-zinc finger protein OsDOF22 The nucleotide sequence is shown as SEQ ID NO. 1, and the corresponding protein amino acid sequence is shown as the sequence table SEQ ID NO. 2. The application reports for the first time that OsDOF22 The gene can regulate the growth of primary roots in the germination stage, and experiments show that the mutation of the gene reduces the length of the primary roots of rice, and the overexpression of the gene improves the root growth ability of rice in the germination stage. It is proved that the application OsDOF22 The gene regulates the length of the primary roots of rice in the germination stage, and the use of the gene is helpful to screening and cultivating high-activity rice varieties and is beneficial to the direct seeding rice production.

The invention discloses the application of the rice zinc finger protein coding gene RDW1 in regulation and control of rice potassium distribution for the first time. The sequence of the rice zinc finger protein coding gene RDW1 is as shown in SEQ ID NO. 1. The gene can influence potassium distribution in the rice body. The biological function of the rice zinc finger protein coding gene RDW1 in the aspect of potassium nutrition is found for the first time through a large number of experiments, and after the gene is knocked out, more K < + > of potassium in rice can be accumulated on the overground part.

This disclosure generally relates to compounds that bind to and act as degraders of IKZF family zinc finger (IKZF) proteins, such as IKZF2 (Helios) and / or IKZF4 (Eos) . This disclosure further relates to the use of such compounds in the preparation of pharmaceuticals for treating diseases and / or conditions (including cancer) by binding to and degrading IKZF proteins, such as IKZF2 and / or IKZF4 .

The invention discloses a zinc finger homologous domain transcription factor GhZHD22 molecular marker related to cotton fiber quality characters and application of the zinc finger homologous domain transcription factor GhZHD22 molecular marker, and belongs to the field of biotechnology application. The SSR molecular marker can be used for detecting two haplotypes of the cotton zinc finger homologous domain transcription factor GhZHD22, and the two haplotypes are respectively named as GhZHD22Gb and GhZHD22Gh. And compared with the haplotype 2, the haplotype 1 has the deletion of a CAC repeated primitive at the 142bp position of the CDS sequence. A to-be-detected cotton germplasm material carrying a zinc finger homologous domain transcription factor GhZHD22Gb with a nucleotide sequence shown as SEQ ID NO.1 is selected as a cotton germplasm with a high fiber quality character and is used for cotton breeding. The gene and the SSR molecular marker thereof have important application prospects in improvement of cotton fiber quality characters and cultivation of new high-quality cotton varieties.

Methods of inducing rejuvenation in a population of adult glial progenitor cells, and methods of treating a subject having a myelin deficiency are disclosed in this patent application. The method of inducing rejuvenation in a population of adult glial progenitor cells may comprise administering, to the population of adult glial progenitor cells, an effective amount of an agent that suppresses one or more transcription factors selected from the group consisting of (i) zinc finger protein 274 (ZNF274), (ii) Myc-associated factor X (MAX), (iii) E2F transcription factor 6 (E2F6), (iv) zinc finger protein Aiolos (IKZF3), and (v) signal transducer and activator of transcription 3 (STAT3).

The application belongs to the technical field of disease treatment, and discloses TRAFD1 induced activation of CD8+ T cell immune response and application in treatment of pancreatic cancer. It is found in the application that TRAF-type zinc finger domain containing 1 gene (TRAFD1) can be used as a molecular marker for predicting whether a pancreatic cancer patient can benefit from immunotherapy. Meanwhile, TRAFD1 promotes the effect of immunotherapy by affecting the activation and effector function of CD8+ T cells, which has important significance for promoting pancreatic cancer immunotherapy.

The application discloses a zinc finger protein ZFP gene and application thereof, and identifies the interaction protein ZFP of BmPGRP2 through yeast two-hybrid screening; the ZFP directly combines with the GACATAGATATT sequence on the PTEN promoter as a transcription factor, and inhibits the transcription of PTEN; the expression amount of PTEN can be significantly increased by knocking out the ZFP, and the proliferation of BmNPV is inhibited. Therefore, the ZFP is a negative regulation factor of PTEN, has important value in the research and application of the silkworm antiviral molecular breeding, and the antiviral ability of the silkworm can be improved by knocking out the gene through gene editing.

Provided herein are isoindolinones, e.g., a compound of Formula (I), and pharmaceutical compositions thereof. Also provided herein are methods of their use for treating, preventing, or ameliorating one or more symptoms of a disorder, disease, or condition mediated by a zinc-figure protein, casein kinase 1α, GSPT1, or PDE6D.

Provided herein are gene repressor systems comprising fusion proteins, such as fusion proteins comprising a DNA binding domain such as a TALE, zinc finger or catalytically-dead CRISPR protein and guide nucleic acid (gRNA), which are useful in the repression of a proprotein convertase subtilisin / kexin Type 9 (PCSK9) gene. Also provided are methods of using such systems to repress transcription of PCSK9.

The present disclosure provides a vector containing a target sequence that targets and interferes with Helicase with zinc finger 2 (Helz2), the target sequence comprising one or more of the following sequences:SEQ ID No. 5:5′-GCTATCAAGTCTGTCACTACT-3′;SEQ ID No. 6:5′-GCACGATGCTGTATGGCTTTG-3′;SEQ ID No. 7:5′-GGGCCTCATTGACACTCAAAG-3′.

Disclosed herein are improved methods and compositions for diagnosing, preventing and / or treating Huntington's disease. Provided herein, inter alia, is a gene therapy construct encoding a non-naturally occurring codon optimized transcription factor (ZFP-TF) comprising a zinc finger protein (ZFP) sequence and a sequence encoding a transcription repression domain wherein the expression of the ZFP-TF is driven by a phosphoglycerate kinase 1 (PGK), ubiquitin C (UBC), EFS, or EF1 [alpha] promoter.

Described herein are pharmaceutical compositions containing genes encoding a positive regulatory domain zinc finger protein 16, a peroxisome proliferator-activated receptor gamma coactivator 1-alpha protein, and a bone morphogenetic protein 7. Also disclosed are methods of treating or managing obesity and diabetes by delivering these pharmaceutical compositions using ultrasound-targeted microbubble destruction.

The present invention relates to a DNA base editor and a base editing method using a nickase and a zinc finger protein. The present invention is useful for correcting bases in nuclear DNA or organellar DNA and, in particular, for correcting bases in DNA of organelles such as chloroplasts or mitochondria. The DNA base editor according to the present invention has a relatively low molecular weight, and thus can be delivered into an organism while being relatively loosely constrained in terms of vector size. In addition, correction can only occur at a single base within a spacer, thus having the advantage of lowering the possibility of bystander correction.