67 results about "Pennisetum" patented technology

The invention discloses a high-yield cold-resistant pennisetum germplasm screening method and application thereof, and the method is based on 10 yield traits, 4 cold-resistant indexes and 4 low-temperature photosynthetic ability indexes of pennisetum, constructs a multi-dimensional dynamic monitoring system across autumn, winter and spring, and performs high-yield cold-resistant comprehensive evaluation on the pennisetum germplasm, thereby screening out a high-yield cold-resistant breeding material. The invention provides a comprehensive, efficient and accurate high-yield cold-resistant Chinese pennisetum germplasm screening method, through a multi-dimensional dynamic monitoring system crossing autumn, winter and spring, yield traits, cold resistance indexes and low-temperature photosynthetic ability indexes are integrated, and the problems that a traditional method is single in evaluation dimension, disjointed with field reality and insufficient in dynamic adaptability are solved. The comprehensive evaluation system based on the principal component quantitative model not only fills the blank of the high-yield and cold-resistant evaluation model of the pennisetum alopecuroides, but also remarkably improves the breeding efficiency, provides powerful support for cultivating cold-resistant and yield-reduction-free breakthrough varieties, and has important application value and popularization prospect.

The invention discloses a pennisetum purpureum gene PpbHLH148L and application thereof in improving the cold resistance of plants, and relates to the technical field of gene engineering. The nucleotide sequence of the gene is as shown in SEQ ID NO.5. After the gene is subjected to subcellular localization and expression condition analysis after cold stress, the gene is related to low-temperature tolerance. An overexpression vector of the gene is constructed, Agrobacterium tumefaciens is transferred, then Arabidopsis thaliana infection is carried out, cold tolerance determination is carried out on overexpressed Arabidopsis thaliana, phenotypic difference and physiological index difference between an overexpressed strain after cold treatment and a wild type are observed, and it is found that the gene can significantly improve the low-temperature stress tolerance of Arabidopsis thaliana. And in the low-temperature treatment of the pennisetum alopecuroides transgenic callus, the transgenic callus also shows higher tolerance than the wild callus at low temperature. The pennisetum purpureum gene PpbHLH148L can be used for low-temperature tolerance modification of plants, and has an application prospect in creation of low-temperature-resistant plant materials.

The invention belongs to the technical field of saline-alkali land improvement, and particularly discloses a saline-alkali land treatment method which comprises the following steps: soil pretreatment, deep ploughing of saline-alkali land soil, leveling of the soil, primary spraying of water to the soil, leaching treatment, improvement of the soil structure and improvement of the soil fertility. According to the method, a base fertilizer is applied within 5-10 days after preliminary improvement and soil leaching treatment, then ploughing is carried out after a modifier is applied, water is sprayed to the soil for the second time after ploughing, and wetting treatment is carried out, so that long-acting nutrients and a good soil microbial environment can be provided for crop growth; alkali-resisting plants are planted, the alkali-resisting plants can be sown into the soil 15-20 days after the soil wetting treatment, and the alkali-resisting plants comprise suaeda glauca, alfalfa, pennisetum sinese or leymus chinensis; the treatment is enhanced, topdressing is applied during the growth period of the alkali-resistant plants, that is, the modifier is applied again, and the topdressing mode adopts furrow application, hole application or flushing application, so that the modifier can quickly reach the periphery of the root system of the crop to play a role.

The invention discloses an intelligent Juncao transplanting method and system based on the Internet of Things technology, and relates to the technical field of intelligent agriculture, the intelligent Juncao transplanting method and system collects key environment data such as soil microbial environment, wind speed and pressure, mechanical stress, soil moisture and illumination conditions in real time, and establishes a transplanting environment prediction model by using the convolutional neural network technology. Therefore, the Juncao transplanting adaptive capacity of each sub-region can be screened, the transplanting success rate can be improved, and resource allocation can be optimized. In the pennisetum sinese transplanting growth process, root exudate compatibility data and pest attraction effect data are continuously collected, a dynamic growth data set is established, and a growth risk coefficient is monitored in real time. In the process, the bad trend in growth can be found in time, and it is ensured that countermeasures are taken at the beginning of the problem. And according to a real-time risk assessment result, an environment optimization strategy is adjusted, healthy growth of the Juncao in the whole growth period is ensured, and unnecessary resource waste is reduced.

The invention discloses a method for improving the yield of hybrid pennisetum and the soil quality of a planting land, which comprises the following steps: measuring nutritional ingredients in biogas slurry of a pig farm, including organic matters, total nitrogen, total phosphorus and total potassium; biogas slurry and chemical fertilizers with different application gradients are designed to keep the nitrogen level stable; through field trials, the yield of the hybrid pennisetum and the physicochemical properties of soil under different treatments are measured so as to determine the maximum safe absorption amount. According to the method, scientific fertilization of the hybrid pennisetum is realized by accurately measuring nutritional ingredients in the biogas slurry of the pig farm and designing the biogas slurry and the chemical fertilizer with different application gradients, so that the yield of crops is remarkably increased. Meanwhile, the maximum safe absorption amount of the biogas slurry is determined through field trials, and a scientific basis is provided for reasonably utilizing livestock and poultry breeding wastes. According to the method, by optimizing application of the biogas slurry, the physical and chemical properties of soil are improved, the soil fertility is improved, resource utilization of agricultural waste is achieved, and sustainable development of ecological agriculture is promoted.

The utility model relates to a carex meyeriana composite fabric forming mechanism and bonding and reinforcing device, including fabric processing machine main part, bonding and reinforcing component, support plate and fabric forming component, the fabric processing machine main part is equipped with bonding and reinforcing component, the inner wall of two sides of fabric processing machine main part is fixedly connected with the support plate, the fabric forming component is equipped with the bonding and reinforcing component, and the bonding and reinforcing component is equipped with the bonding and reinforcing component. A fabric forming assembly is mounted on the supporting plate and the fabric processing machine main body; the bonding and reinforcing assembly comprises an adhesive pipe, a discharging hole, a supporting frame, a first motor, a sealing baffle disc and a sealing rotating wheel; according to the utility model, the bonding and reinforcing assembly is adopted, the spinning layer can be automatically glued, and the glued spinning layer is bonded and reinforced, so that manual assistance is reduced, and the bonding and reinforcing effect of the fabric is improved; and the fabric forming assembly is adopted, during heating forming, the edge of the fabric can be cut, manual auxiliary cutting is not needed, the cutting burden is reduced, and the fabric cutting effect is improved.

The invention discloses an application of a pennisetum whole genome InDel molecular marker in variety identification. According to the invention, firstly, 108 new InDel markers are screened, and the markers can be used for intraspecific identification of four cultispecies of pennisetum alopecuroides, Yu valley, pennisetum purpureum and hybrid pennisetum alopecuroides, and can also be used for interspecific identification, so that the specific species category of a to-be-detected sample can be clearly identified; and the method has a wide application prospect in the aspects of variety identification, variety right dispute judgment, auxiliary market dispute and the like.

The utility model relates to the technical field of pennisetum sinese planting, in particular to a pennisetum sinese seedling heat preservation device applied to a greenhouse, which comprises a plurality of supporting rods, a plurality of connecting pieces, a plurality of heat preservation films, a cross beam and a suspension piece. The supporting rods and the connecting pieces play a role in supporting and fixing the heat preservation film, so that the heat preservation film can stably cover the seedling growing and planting area, and pennisetum sinese seedlings are planted and cultivated in the seedling growing and planting area. The height position of the heat preservation film can be adjusted by connecting the heat preservation film to the connecting pieces at different heights. Due to the fact that the growth speed of the pennisetum sinese is high, and the height position of the heat preservation film is adjustable, the device can adapt to pennisetum sinese seedlings of different heights. The cross beams can play a role in stabilizing and fixing the supporting rods and provide bearing for the hanging pieces, the hanging pieces hang the heat preservation film from the upper portion, the middle of the heat preservation film is prevented from collapsing, the position of the heat preservation film is further stabilized, and the heat preservation film is prevented from pressing seedlings to a certain extent while the heat preservation film plays a role in preserving heat of the pennisetum sinese seedlings.

The application provides a method for identifying and screening cold tolerance of elephant grass and application thereof, and relates to the field of plant cultivation. The method mainly comprises the following steps: subjecting the standard elephant grass stem segments to variable temperature germination; subjecting the standard elephant grass stem segments to constant temperature low temperature stress; subjecting the stressed stem segments to recovery culture under suitable conditions; determining and calculating the low temperature germination, stress survival rate and recovery growth rate of the stem segments; and comprehensively evaluating the cold tolerance of the stem segments based on the weighted values of the three. The application overcomes the shortcomings of the prior art, can simulate the key low temperature stress and recovery process in the laboratory, and can realize rapid, reliable and batch preliminary identification and screening of the cold tolerance of a large amount of materials, so that the blind dependence on large-scale and long-period field identification is significantly reduced, the screening accuracy and breeding efficiency are effectively improved, and the breeding period is shortened.

The application relates to SRAP molecular marker primers for genetic diversity analysis of pasture of the Puccinellia genus and a genetic diversity analysis method thereof; the SRAP molecular marker primers are composed of the following eight pairs of primers: ME1-EM5, ME2-EM3, ME3-EM5, ME4-EM3, ME5-EM2, ME6-EM7, ME7-EM2 and ME8-EM2. The eight pairs of SRAP molecular marker primers screened by the application have the advantages of rich amplification bands, rich polymorphism, large brightness and good repeatability, and can be used for genetic diversity analysis of the Puccinellia genus pasture varieties.

The application discloses a sub-genome-specific retrotransposon of elephant grass and application thereof, and belongs to the field of bioinformatics and molecular cytogenetics. The application mainly comprises the following steps: 2 sub-genome-specific retrotransposon sequences are screened out through bioinformatics analysis of short fragment sequences of the sub-genome of elephant grass; primers are designed according to the sequences, and the genomic DNA of elephant grass is used as a template to amplify the primers through PCR, and the target fragments are recovered through purification of PCR products; and it is verified that the 2 retrotransposon sequences produce clear and bright signals on the respective 14 chromosomes of elephant grass through probe labeling, chromosome sheet preparation and fluorescence in situ hybridization. The retrotransposon sequences can be directly used for specific identification of the sub-genome of elephant grass, more accurate information is provided for identification of the sub-genome of elephant grass, and a foundation is laid for chromosome engineering breeding of elephant grass.

This invention belongs to the field of silage machinery and equipment, specifically relating to an adjustable feeding and chopping device and its control method for a hybrid Napier grass harvester. The feeding and chopping device includes a shell, conveying components, a gathering component, a pre-compression component, a feeding component, and a chopping component; the pre-compression component includes an upper pressure roller, a slider, a pin, a limit slot, a front slide rail, a rear slide rail, and a pressure sensor. This device can automatically adjust the forage gathering range, feeding speed, and chopping speed according to the amount of crop fed. It can pre-compress loose stalks in advance to improve the chopping effect. It can also reasonably adjust the power input according to the feeding amount to reduce energy loss, further improving the quality and efficiency of silage chopping. The front top cover and pre-compression component enable the device to quickly clear blockages, reducing problems caused by material blockage.

The invention relates to the technical field of silage, and provides a pennisetum hydridum silage additive which comprises the following raw materials in percentage by mass: 10-15% of table salt, 40-70% of a microbial fermentation agent, 5-10% of an enzyme preparation, 10-15% of antioxidant active lactic acid bacteria and 5-10% of a degradation agent. The bacillus licheniformis disclosed by the invention can secrete lichenin, can quickly consume free oxygen in the environment in an aerobic state to generate a strong biological oxygen abstracting effect, and is matched with the bacillus subtilis to quickly create an anaerobic environment and promote the growth of anaerobic bacteria and lactic acid bacteria, and meanwhile, enzymes are generated to decompose cellulose, so that the content of fibrous substances is reduced.

The invention discloses a flue-cured tobacco bio-organic fertilizer preparation method, which comprises: crushing pennisetum sinese straw, carrying out decomposition fermentation aging to obtain straw powder, mixing the straw powder with a nitrogen-phosphorus-potassium compound material to obtain a mixture, adding the mixture to an alkali liquid, heating to a boiling temperature of 53 DEG C, maintaining the temperature for 25-30 min, carrying out solid-liquid separation, and drying the obtained solid to obtain a solid product A, adjusting the pH value of the liquid obtained after solid-liquid separation in the previous step to be neutral, continuing solid-liquid separation, drying the solid to obtain a solid product B, uniformly mixing the solid product A and the solid product B with a microbial agent, ammonium carbonate, flue-curing barn biomass fuel ash, calcium superphosphate and tobacco stem ash, and stacking and fermenting to obtain the flue-cured tobacco bio-organic fertilizer. The pennisetum sinese is prepared into the bio-organic fertilizer, the organic matter content can be obviously increased, the water retention capacity of the fertilizer is improved, the pennisetum sinese is a porous substance, and after the substance is applied, the air permeability, the water permeability and the fertilizer retention capacity of the fertilizer are good.

The invention belongs to the field of silage machinery technical equipment, and particularly relates to an adjustable feeding and chopping device of a hybrid pennisetum harvester and an adjusting method of the adjustable feeding and chopping device. The feeding and chopping device comprises a shell, a conveying component, a gathering component, a pre-pressing component, a feeding component and a chopping component; the pre-pressing component comprises an upper pressing roller, a sliding block, a plug pin, a limiting clamping groove, a front sliding rail, a rear sliding rail and a pressure sensor. According to the device, the forage gathering range, the feeding speed and the chopping speed can be automatically adjusted according to the crop feeding amount, fluffy stalks can be pre-pressed in advance, the chopping effect is improved, power input can be reasonably adjusted according to the feeding amount so that energy loss can be reduced, the silage chopping quality and efficiency are further improved, and the device is suitable for popularization and application. The front top cover and the pre-pressing component enable the device to have a quick blockage clearing function, and troubles caused by blockage are reduced.

The invention relates to a carbon reduction and decarbonization method and system for a coal-fired power plant. The carbon reduction and decarburization method for the coal-fired power plant comprises the following steps: S1, ammonia-process carbon capture: capturing carbon dioxide in flue gas by using ammonia water through a decarburization tower to generate an ammonium bicarbonate solution; s2, carbon sequestration of the pennisetum sinese, diluting the solution, using the diluted solution as a liquid nitrogen fertilizer for growth of the pennisetum sinese, using carbon dioxide in tail gas of a decarburization tower as a gas fertilizer through an automatic industrial planting system, and using a byproduct in a carbon capture process as a nitrogen fertilizer; and S3, biomass blending combustion is conducted, the mature pennisetum sinese is naturally aired and dried through boiler waste heat to be prepared into biomass fuel, and the biomass fuel replaces part of fire coal to be put into a power plant boiler for biomass blending combustion power generation. According to the carbon reduction and decarburization method for the coal-fired power plant, provided by the invention, triple carbon reduction technologies of carbon dioxide capture, plant carbon sequestration and biomass energy blending combustion are integrated, so that a closed-loop deep decarburization system is formed; while the carbon capture efficiency is improved, clean energy replacement and resource utilization are realized.

The invention belongs to the technical field of comprehensive utilization of Juncao, and particularly relates to a method for preparing furfural from Juncao and comprehensively utilizing the furfural. The method comprises the following steps: firstly, carrying out catalyst impregnation pretreatment on Juncao, carrying out hydrolysis reaction by using an integrated vertical hydrolysis pot, and separating after hydrolysis to obtain a furfural aqueous solution and a blow-off material, namely furfural residue; cooking the blown material through alkaline pulping, squeezing, washing with water, and bleaching to obtain finished pulp; black liquor obtained in the material spraying and squeezing process reacts by a multi-effect evaporator to obtain lignin; therefore, production of Juncao furfural and effective separation of pulp and lignin are realized.

The invention provides a deep eutectic solvent as well as preparation and application thereof. The deep eutectic solvent is prepared from choline chloride, p-toluenesulfonic acid, 1, 4-butanediol and water, wherein the molar ratio of choline chloride to p-toluenesulfonic acid to 1, 4-butanediol is (1-2): (3-6): (2-8), and the ratio of the total mass of choline chloride and p-toluenesulfonic acid to the mass of water is 20: (9-11). When the eutectic solvent is used for pretreating the pennisetum hydridum, hemicellulose and lignin on cell walls of the pennisetum hydridum can be effectively removed, and then the enzymolysis efficiency of cellulose of the pennisetum hydridum is remarkably improved.

The invention relates to the technical field of fermented feeds, in particular to a pennisetum sinese bacterium enzyme synergistic fermented feed as well as a preparation method and application of the pennisetum sinese bacterium enzyme synergistic fermented feed. The invention discloses a pennisetum sinese bacterium enzyme synergistic fermentation method. The method comprises the following steps: S1, crushing pennisetum sinese sections, and sieving to obtain micronized pennisetum sinese; s2, mixing saccharomyces cerevisiae, bacillus coagulans, lactobacillus casei, cellulase and water to obtain a zymophyte aqueous solution; s3, mixing the zymophyte aqueous solution with the micronized pennisetum sinese, and performing solid-state fermentation to obtain the pennisetum sinese bacterium enzyme synergistic fermentation feed. According to the application, the saccharomyces cerevisiae, the bacillus coagulans, the lactobacillus casei and the cellulase are used for synergistically fermenting the pennisetum sinese, so that the dry matter degradation rate and the crude protein content of the pennisetum sinese are increased, the contents of crude fibers and tannin are reduced, and the growth performance and the immunity of pigs and poultry are improved.

The invention relates to a method for rapidly regreening acid mine soil through energy plants. The method for rapidly regreening acid mine soil through the energy plants is implemented through the following steps that S1, mine soil treatment is conducted, specifically, a soil conditioner is evenly spread on the exposed to-be-restored earth surface of a mine; s2, preparing a water-retaining matrix; s3, planting of energy plants: digging planting holes, putting a water-retaining substrate, performing cuttage to cultivate seedlings, and transplanting the seedlings into the planting holes; and S4, the ground cover grass seeds are sown to fully cover the ground surface. According to the method for rapidly regreening the acid mine soil by planting the energy plants, the acid soil is subjected to acid-base neutralization and restoration treatment by adopting organic fertilizer, lime and a conditioner, then the fast-growing energy plant pennisetum hydridum is planted to harvest and manufacture a large amount of biomass energy raw materials, and the other bare earth surface is covered with broadcast-sown screened ground cover herbs, so that the energy plants are planted to rapidly revive the acid mine soil. Therefore, the purposes of soil restoration, water and soil conservation, energy biomass production and other ecological economy are achieved.

The invention provides a method for planting gastrodia elata by replacing firewood with pennisetum sinese. The planting method comprises the following steps: step 1, position selection; the method comprises the following steps: selecting a land parcel with loose, breathable and water-permeable soil and a shading degree of 45% in a mountainous area with an altitude of 600-1800m, and scarifying and leveling the land parcel; step 2, fungus material preparation; harvesting the mature pennisetum sinese, pulling the mature pennisetum sinese to the field, cutting straw into 15 cm for later use, and cutting straw leaves into 7 cm for later use. Step 3, gastrodia elata planting; step 4, burying the box body; the gaps around the planting box are backfilled with soil, the soil is compacted forcibly, and the part, protruding out of the ground, of the side wall of the planting box is covered with the soil as well. And step 5, gastrodia elata harvesting. According to the invention, the adjustable planting device is used. The cover plate is lifted through a threaded structure, internal fungus materials can be fixed by moving downwards, and cover opening and ventilation gap forming are facilitated by moving upwards. The method utilizes the pennisetum sinese to completely replace wood, and is environment-friendly and efficient.

The invention relates to a novel low-temperature type erythromelastoma sanguineum strain TsJC206 and a pennisetum hydridum cultivation method thereof. According to the TsJC206, one or more Juncao such as pennisetum sinese and bamboo reed can be used as main raw materials of a culture medium, even fresh pennisetum sinese and fresh bamboo reed can be used for cultivation, the content of triterpenes in sporocarps reaches 1.15%, the content of polysaccharides reaches 10.82%, and the biological conversion rate reaches 25%; secondly, the strain is a low-temperature strain, the ganoderma lucidum growing temperature is 15-20 DEG C and is obviously lower than the ganoderma lucidum growing temperature reported in the prior art, and the ganoderma lucidum growing warm type of the red trametes is supplemented; and 3, the basic group of the 182nd site of the ITS sequence in the NCBI database is G, and the basic group is different from the basic group A of the OR062414.1 with the highest similarity in the NCBI database at the 233rd site. The cultivation method comprises the steps of mother seed preparation, stock seed preparation, cultivation and ganoderma lucidum emergence management. The method has the beneficial effects that ganoderma lucidum can be grown at a lower temperature of 15-20 DEG C; the green, organic and sustainable raw material of Juncao can be used for cultivating the red trametes; the fresh fungus grass can be used for replacing part of the compost, the step of drying the compost is omitted, and cost is reduced.

The utility model relates to the field of miscanthus transferring equipment, and provides a miscanthus transplanting and transferring box which comprises a box body and a plurality of transplanting discs stacked in the box body, each transplanting disc comprises a water retention layer, a disc body and supporting blocks fixedly connected to the two sides of the disc body, the water retention layer is attached to the upper end face of the disc body, and each supporting block comprises a first block body and a second block body. The first block body protrudes out of the water retention layer, and the second block body protrudes out of the lower end face of the disc body. According to the miscanthus amurensis transplanting and transferring box provided by the utility model, the miscanthus amurensis turfs are flatly laid by arranging the transplanting discs, and the stacking of the miscanthus amurensis turfs is completed by stacking the transplanting discs. The space utilization rate is increased, meanwhile, damage to miscanthus is reduced, and the survival rate of miscanthus transplanting is increased. The water retention layer is arranged on the upper end face of the tray body, the water retention layer is wetted so as to provide water for turf of the miscanthus on the water retention layer, and the survival rate of the miscanthus is further increased.

The invention belongs to the technical field of breeding, and particularly relates to a compound feed added with fresh pennisetum sinese and used for pigs and a preparation method and application of the compound feed. The feed consists of 40-50 parts by weight of fresh pennisetum sinese and 50-60 parts by weight of basic feed for pigs. Harvesting the fresh pennisetum sinese when the plant height is 1.5 m, and chopping to be less than 1cm; the basic feed comprises corn, soybean meal, premix and other raw materials, and the premix provides various vitamins and minerals. During preparation, the fresh pennisetum sinese and the basic feed are uniformly mixed according to the proportion. The feed is applied to pig breeding, and tests show that in the feeding period of 90 days, the added fresh pennisetum sinese can partially replace a basic feed and reduce the feed intake of the basic feed, the daily average weight gain of pigs is similar to that of a group without the fresh pennisetum sinese, the feed has no significant adverse effect on the growth performance, and the feed has the advantages of saving the feed cost and improving the resource utilization rate.

The invention discloses a plasma mutation breeding method for callus of hybrid pennisetum alopecuroides' Bangdel No.1 '. According to the method, the callus induced by the Bangdel No.1 seed is used as a mutagenesis base material, an ARTP radiation mutagenesis technology is combined with plant tissue culture, and the mutagenesis effect of ARTP radiation on the hybrid pennisetum callus is explored from different aspects of phenotype, physiology and molecules in different stages of callus growth and plant regeneration. Furthermore, a low-temperature stress test is carried out on the regenerated plant, the morphological and physiological index differences of a mutagenesis plant and a control plant before and after low-temperature stress treatment are observed, mutagenesis strains with cold-resistant potential in the present generation are screened, and the cold-resistant hybrid pennisetum germplasm is obtained. The invention provides theoretical and material basis for the breeding of new germplasm of cold-resistant hybrid pennisetum alopecuroides.

This utility model relates to the field of everyday footwear technology. It discloses a Ula grass slipper, comprising, from bottom to top, an outer sole, a support layer, a Ula grass ecological felt layer, and an inner sole. The edge of the outer sole extends upwards to form a edging, and one side of the edging is connected to the upper. The upper is connected to the edge of the inner sole. This Ula grass slipper, with its natural fiber blended knitted fabric used for the Ula grass ecological felt layer, upper, and inner sole, has excellent breathability, effectively dissipating sweat and preventing dampness and stuffiness. This solves the problem of poor breathability in traditional slippers. Ula grass itself has natural antibacterial and deodorizing properties. The Ula grass ecological felt layer further enhances the slipper's antibacterial and deodorizing functions, effectively inhibiting the growth of bacteria and fungi, reducing the occurrence of foot diseases such as athlete's foot, satisfying people's pursuit of a healthy life, and compensating for the lack of antibacterial and deodorizing functions in traditional slippers.

The invention belongs to the field of silage, and particularly relates to a preparation method of hybrid pennisetum silage, which comprises the following steps: S1, uniformly mixing a hybrid pennisetum raw material with lactobacillus plantarum LP276 and cellulase to obtain a silage raw material; s2, fermenting the silage raw materials at the temperature of 20-30 DEG C for not less than 45 days; wherein the lactobacillus plantarum LP276 is preserved in the Sichuan Province Microbial Resource Platform Culture Collection Center, and the preservation number is SICC 1.384. The preparation method provided by the invention breaks through the disadvantage that the traditional pennisetum alopecuroides silage is easy to decay, realizes the effects of effectively preserving the nutritional ingredients and improving the animal palatability and feeding value, can partially replace high-quality roughage such as silage corn and the like, and reduces the feeding cost of beef cattle and mutton sheep.

The invention discloses a Yu-grain and pennisetum alopecuroides protoplast fusion and callus regeneration method, which comprises the following steps: step 1, callus suspension cell culture: using young ears of Yu-grain and pennisetum alopecuroides to induce granular calluses, and establishing a callus suspension cell line; step 2, protoplast separation and purification: taking the suspension cell line as a material, carrying out protoplast separation by an enzymolysis method, and purifying the protoplast by a filtration-centrifugation method; step 3, protoplast fusion and callus regeneration: fusing the Yu valley protoplast and the pennisetum alopecuroides protoplast by using an electric fusion method; and taking the fused protoplast, and carrying out moisturizing culture at 27-28 DEG C in the absence of light / scattered light to obtain the regenerated callus of the fused protoplast. All the steps involved in the method are a whole set of culture system, all links are tightly connected to form a whole, and a foundation is laid for obtaining a pennisetum interspecific protoplast fusion regeneration plant.

The present application relates to a kind of industrial juicer for deep processing of pennisetum, comprising: frame, feed hopper, pretreatment zone and residue-liquid separation zone are arranged in height on frame;Roller feeding mechanism, for material is rolled and delivered to pretreatment zone;Cutting material roller mechanism, including cutting plate being arranged in pretreatment zone, the outside of cutting plate can be movably installed cutting blade, and the bottom side of cutting blade is installed broken material compression roller;Hot softening guide mechanism, for heating softening treatment to material after roller pressing and conveying it to centrifugal separation zone;Juice separation device, including primary filter cartridge and secondary filter cartridge, the top of the secondary filter cartridge is closed and fixed with end cover plate, a plurality of residue outlets are formed in end cover plate, and lifting auger is rotatably arranged in the middle of end cover plate;Juice collection mechanism, for discharging and collecting juice.The present application can facilitate feeding and overall smooth progress of juicing process, and can effectively improve juicing effect and juicing efficiency.

The utility model relates to the technical field of seed screening, and discloses an American pennisetum seed grading and screening device which comprises a screening machine, a grading and screening mechanism and a segmented conveying mechanism, a screening box is movably arranged at the top of the screening machine, and the grading and screening mechanism is arranged in the screening box; through a connecting plate and a discharging channel, unfiltered seeds can be conveniently guided into a guide cylinder through a feeding groove, a motor is matched to conveniently rotate an auger clockwise, the auger can conveniently drive the unfiltered seeds to be conveyed upwards, and meanwhile, a third screening plate is matched to conveniently convey and guide the seeds in a segmented mode; according to the seed screening device, seeds directly fall on the second screening plate through the third screening plate for secondary screening, the filtered seeds directly fall into the screening box from the second screening plate, and the filtered seeds are discharged from the first discharging plate through guiding of the second screening plate, so that the seeds are fully screened, and the screening effect is better.