7 results about "Pennisetum" patented technology

The application provides a method for identifying and screening cold tolerance of elephant grass and application thereof, and relates to the field of plant cultivation. The method mainly comprises the following steps: subjecting the standard elephant grass stem segments to variable temperature germination; subjecting the standard elephant grass stem segments to constant temperature low temperature stress; subjecting the stressed stem segments to recovery culture under suitable conditions; determining and calculating the low temperature germination, stress survival rate and recovery growth rate of the stem segments; and comprehensively evaluating the cold tolerance of the stem segments based on the weighted values of the three. The application overcomes the shortcomings of the prior art, can simulate the key low temperature stress and recovery process in the laboratory, and can realize rapid, reliable and batch preliminary identification and screening of the cold tolerance of a large amount of materials, so that the blind dependence on large-scale and long-period field identification is significantly reduced, the screening accuracy and breeding efficiency are effectively improved, and the breeding period is shortened.

This utility model relates to the field of everyday footwear technology. It discloses a Ula grass slipper, comprising, from bottom to top, an outer sole, a support layer, a Ula grass ecological felt layer, and an inner sole. The edge of the outer sole extends upwards to form a edging, and one side of the edging is connected to the upper. The upper is connected to the edge of the inner sole. This Ula grass slipper, with its natural fiber blended knitted fabric used for the Ula grass ecological felt layer, upper, and inner sole, has excellent breathability, effectively dissipating sweat and preventing dampness and stuffiness. This solves the problem of poor breathability in traditional slippers. Ula grass itself has natural antibacterial and deodorizing properties. The Ula grass ecological felt layer further enhances the slipper's antibacterial and deodorizing functions, effectively inhibiting the growth of bacteria and fungi, reducing the occurrence of foot diseases such as athlete's foot, satisfying people's pursuit of a healthy life, and compensating for the lack of antibacterial and deodorizing functions in traditional slippers.

The application belongs to the technical field of plant transgenics, and particularly relates to a nano-magnetic-bead-mediated pollen tube channel transgenic method of Pennisetum. The method comprises the following steps: magnetic bead and plasmid DNA complexing, magnetic bead-DNA complex and pollen culture solution complexing, magnetic transfection, and pollination. Compared with a traditional transformation mode, the nano-magnetic-bead-mediated transformation method does not need to pass through tissue culture, the transformation condition is more moderate, the time is shorter, and the method is a high-efficiency and rapid transformation mode, which has great guiding significance for the genetic breeding work of the Pennisetum. A large amount of seeds can be obtained through artificial pollination, and more materials are provided for the screening of subsequent transgenic plants. The method is simple in operation, saves resources, and has a synergistic effect between the operation steps, and excellent effects are achieved.

This invention discloses a transcription factor gene PaZFP36 in *Lycoris radiata* that enhances plant cold resistance and yield, and its application. This invention belongs to the field of biotechnology. The protein PaZFP36 is any one of the following: A1) a protein with the amino acid sequence shown in SEQ ID No:2; A2) a protein obtained by substituting and / or deleting and / or adding amino acid residues to the protein of A1), possessing more than 80% identity and the same function as the protein shown in A1); or a fusion protein obtained by attaching a protein tag to the N-terminus and / or C-terminus. Overexpression in wild-type *Lycoris radiata*. PaZFP36 The gene can significantly improve the cold resistance and yield of 'Liqiu' Napier grass, which has important production application value for creating parent plants with excellent cold resistance and high yield characteristics and for breeding cold-resistant and high-yielding varieties.

The utility model discloses a kind of uralsit cushion mould pressing forming mechanism, including base and heating mechanism, the top end one side of the base is provided with equipment box, and the top end of equipment box is provided with controller, the top end other side of the base is provided with organism, and the top end both ends of organism are all provided with hydraulic press, the top end of hydraulic press is provided with bottom pressing table, the surface of bottom pressing table is provided with heating mechanism.The uralsit cushion mould pressing forming mechanism, heating mechanism is set, when uralsit cushion is mould pressed and formed by equipment, the heater in heating mechanism is heated by the heating plate in the heater, heat is conducted to the forming die installed, so that the forming die is heated, so when mould pressing and forming, uralsit material can be heated and softened by heat, so that it is easier to press and form, so as to improve the efficiency of mould pressing and forming.

The application discloses a kind of agrobacterium-mediated'Bond No. 1'hybrid pennisetum genetic transformation method. The method comprises the following steps: using maltose solution containing glutamine to pretreat hybrid pennisetum embryonic callus;Agrobacterium resuspension solution containing the vector of target gene is used to infect pretreated callus;The callus after infection is inoculated into co-culture medium, recovery medium, screening medium, differentiation medium and rooting medium in sequence for culture, and regeneration transformation plant is obtained. The application successfully establishes agrobacterium-mediated'Bond No. 1'hybrid pennisetum genetic transformation system, and the positive rate can reach 62%, and'Bond No. 1'hybrid pennisetum efficient and stable genetic transformation is first effectively realized, and the analysis of hybrid pennisetum gene function research and the excavation of key candidate gene are laid the foundation for hybrid pennisetum molecular breeding.