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5 results about "Molecular cytogenetics" patented technology
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Molecular cytogenetics involves the combination of molecular biology and cytogenetics; using various reagents, molecular cytogenetics seeks to properly distinguish normal and cancer-causing cells. Molecular cytogenetics is a useful tool for the diagnosing and treatment of various malignancies such as brain tumors, haematological malignancies, etc. It includes a series of techniques referred to as fluorescence in situ hybridization, or FISH, in which DNA probes are labeled with different colored fluorescent tags to visualize one or more specific regions of the genome. FISH can either be performed as a direct approach to metaphase chromosomes or interphase nuclei. Alternatively, an indirect approach can be taken in which the entire genome can be assessed for copy number changes using virtual karyotyping. Virtual karyotypes are generated from arrays made of thousands to millions of probes, and computational tools are used to recreate the genome in silico.
Potato ploidy identification method and ploidy detection kit for marking and counting chromosome centromere based on potato CENH3 antibody
The invention relates to the field of molecular cytogenetics and potato ploidy breeding, in particular to a potato ploidy identification method for marking and counting chromosome centromere based on a potato CENH3 antibody and a ploidy detection kit. According to the method, the chromosome centromere is marked by specifically recognizing the antibody of the potato centromere nucleosome core histone CENH3 and combining the immunofluorescence technology, so that the accurate counting of the chromosome centromere is realized at the single cell level, and the ploidy of potatoes is judged. The method comprises the following steps: preparing a leaf cell suspension, sequentially incubating a potato CENH3 antibody and a labeled secondary antibody after immobilization, and observing and counting by a microscope after redyeing. The method has the advantages of high species specificity, high throughput and low cost, is not limited by special material taking parts of root tips or shoot tips and cell division periods, can accurately identify the uploid and aneuploid of potatoes, and provides an efficient tool for potato germplasm resource evaluation and cross breeding.
Owner:SHENZHEN RESEARCH INSTITUTE OF NORTHWEST A & F UNIVERSITY
Probe, method for efficiently recognizing glycyrrhiza chromosome and application of method
The invention discloses a probe, a method for efficiently recognizing licorice chromosomes and application of the probe, and belongs to the field of bioinformatics and molecular cytogenetics. According to the invention, a single copy oligonucleotide sequence of No. 1-8 chromosomes of liquorice is screened by using bioinformatics, and a chromosome specific oligonucleotide probe pool is constructed through an artificially designed universal primer sequence tag splicing strategy. Through multiple rounds of FISH technology, the chromosomes 1-8 of liquorice can be efficiently and accurately identified. The method provided by the invention solves the problem of difficulty in accurate identification caused by tiny morphology and high similarity of the glycyrrhiza chromosome, has the advantages of high resolution, strong specificity, capability of identifying a single chromosome and the like, and provides a powerful tool for follow-up glycyrrhiza chromosome karyotype analysis, evolution research and exploration of a species formation mechanism.
Owner:SHIHEZI UNIVERSITY
Peanut chromosome centromere specific molecular marker and application thereof
PendingCN122081543AMicrobiological testing/measurementDNA/RNA fragmentationNucleotideNucleotide sequencing
The invention discloses a peanut chromosome centromere specific molecular marker and application thereof, particularly relates to the technical field of molecular cytogenetics, and aims to solve the technical problem that peanut chromosome centromere is difficult to accurately recognize in the prior art. According to the specific molecular marker of the peanut chromosome centromere, the molecular marker comprises CentAh106 or CentAh342; the nucleotide sequence of the CentAh106 is as shown in SEQ ID NO. 1, and the nucleotide sequence of the CentAh342 is as shown in SEQ ID NO. 2.
Owner:CROP RES INST GUANGDONG ACAD OF AGRI SCI
Hybrid probe mixed solution for radiation biological dose detection and preparation method and product thereof
PendingCN122081478AMicrobiological testing/measurementDNA/RNA fragmentationHybridization probeHigh radiation
The invention relates to a hybridization probe mixed solution for radiation biological dose detection as well as a preparation method and a product thereof, and belongs to the field of molecular cell genetics. The technical problem to be solved is to provide a hybridization probe mixed solution with significantly improved chromosome distortion recognition accuracy and sensitivity, higher detection efficiency and better repeatability. According to the key point of the technical scheme, the hybridization probe mixed solution is composed of a probe and a hybridization buffer solution, the volume ratio of the probe to the hybridization buffer solution is 1: (50-1000); the hybridization buffer solution is a 40%-70% formamide solution containing 10 to 20 mM of Tris-HCl or PBS (Phosphate Buffer Solution). When the hybridization probe mixed solution is used for radiation biological dose detection, the distortion identification accuracy and sensitivity are high, the radiation dose estimation precision is high, and the operation is convenient.
Owner:NAT INST FOR RADIOLOGICAL PROTECTION & NUCLEAR SAFETY CHINESE CENT FOR DISEASE CONTROL & PREVENTION
Fluorescent labeled probes for identifying centromeric region of chromosome of pennisetum sinense and application thereof
ActiveCN116287361BMicrobiological testing/measurementDNA/RNA fragmentationIn situ hybridisationGenetics
The present application relates to the fluorescence marker for identifying the centromere region of the chromosome of P. wallichii and its application, belongs to the technical field of molecular cytogenetics, the fluorescence marker probe is named HS-CHTZ5, the probe belongs to the centromere region repeat sequence of P. wallichii, can be combined with the centromere of P. wallichii chromosome and show fluorescence signal, and the centromere segment of P. wallichii chromosome is identified.The fluorescence marker provided by the present application can be used as a beneficial supplement to the prior art, and through in situ hybridization, the efficient identification of the centromere region of the chromosome of P. wallichii is realized, and the present application has important significance for cytogenetic map construction, chromosome karyotype analysis and different genus and species chromosome relationship analysis.
Owner:HENAN UNIV OF SCI & TECH