The invention relates to a method for early identification of cultivated peach fruit shape based on PCR technology. The PCR detection of the present invention uses 4 pairs of primers, including 1 pair of primers (P1) for identifying INV56F (Flat), and 3 pairs of primers (P2/3/4) for identifying INV56R (Round). The identification method is to use the peach genomic DNA to be tested as a template, first use P1 and P2 to amplify respectively, and detect the amplified products. If two specific amplification bands of 1391bp and 705bp are obtained, the fruit shape is flat; if only If there is a 705bp specific amplification band, the fruit shape is round; if no 705bp band is obtained, continue to amplify with the P3 primer pair, if a 981bp specific amplification product band is obtained, the fruit shape is round, otherwise Continue to amplify with the P4 primer; if a 651bp specific amplification product band is obtained, the fruit shape is round, otherwise the fruit shape cannot be judged temporarily. The method has high primer specificity, and the setting of control primers can effectively prevent the occurrence of false negatives, and has the characteristics of simple operation, rapid identification, and accurate results.