4 results about "Metaphase" patented technology

The invention provides an improved euchiloglanis kishinouyei chromosome preparation method, which comprises the following steps: gradually raising the temperature and injecting PHA twice: in the euchiloglanis kishinouyei temporary culture process, raising the water temperature from 18 DEG C to 20 DEG C within 12 hours, injecting a first needle of PHA, raising the temperature to 22.5-23 DEG C after 12 hours, injecting a second needle of PHA, and keeping the water temperature at 22.5-23 DEG C unchanged; after the PHA is injected for the second time for 3 hours, colchicine is injected; sampling: washing the head and kidney in normal saline, and collecting cells; 0.5% of KCl is used for hypotonic treatment; fixing with a Carnoy's fixing solution, and centrifuging; dropping by a cold dropping method and naturally drying; and carrying out Giemsa dyeing and flaking. According to the method, the euchiloglanis kishinouyei somatic cell proliferation is promoted through heating and twice PHA injection, so that the metaphase division phase is increased, the form is clear and dispersed, and the karyotype quality and the preparation success rate of the chromosome are remarkably improved.

This application relates to a chromosome image processing method, apparatus, computer device, computer-readable storage medium, and computer program product. The method includes: acquiring an input image containing multiple chromosomes in metaphase; extracting individual chromosome images from the input image; performing feature analysis on each chromosome based on its image to obtain multidimensional chromosome feature information; and determining the phase quality assessment result of the input image based on statistical information corresponding to the multidimensional chromosome feature information. This method can improve the accuracy of chromosome image processing.

The invention discloses a method for preparing tobacco metaphase chromosomes by utilizing young tobacco plants and application, and belongs to the technical field of preparation of tobacco chromosome specimens. The method comprises the following steps: germinating tobacco seeds at room temperature for 5-7 days, stripping seed shells when the seeds germinate and radicles grow to about 2 mm, sequentially performing N2O treatment on seedlings with radicles, fixing with a stationary liquid, performing enzymolysis in an enzymatic hydrolysate, lightly grinding plant tissues by using a sterile grinding rod until cells are dispersed, centrifuging, airing, adding glacial acetic acid, performing uniform vortex mixing, and performing freeze drying to obtain the tobacco seed suspension. And dripping a small amount of suspension on a clean glass slide to prepare the tobacco metaphase chromosome specimen. According to the method, materials are easy to obtain, the period is extremely short, operation is safe, the chromosome quality is high, and a reliable and efficient standardized scheme is provided for tobacco cytogenetics, molecular cytology and breeding research.

The invention belongs to the technical field of cell culture, and discloses a bone marrow cell culture medium and a preparation method thereof, and a method for in-vitro culture of bone marrow cells, the bone marrow cell culture medium is composed of a basic culture medium, streptomycin, gentamicin, a platelet lysis buffer, and glutamate enzyme; the final concentration of streptomycin is 50-100 [mu] g / mL, the final concentration of gentamicin is 20-50 [mu] g / mL, the final concentration of platelet lysate is 5-15% (v / v), and the final concentration of glutamate enzyme is 0.1-1 mmol / L. The bone marrow cell culture medium can efficiently support in-vitro proliferation of bone marrow cells, through specific combination and appropriate concentration control of the culture medium, the medium-term cell acquisition amount can be remarkably increased, and medium-term cell chromosomes are complete in morphology and good in dispersity; the standard requirements of clinical bone marrow cell chromosome karyotype analysis, blood disease diagnosis and cell genetics experiments in the scientific research field can be met.