56 results about "Metaphase" patented technology

A method of cellular evaluation based on the electronic nature of cells is reveled though cellular reproductions use of a magnetic force. The dynamic process of nuclear response is shown to be electronic in nature relative to DNA mediating electrons hydrogen bonding in bases pairing of DNA though out the a cell cycle and finally during metaphase one see the magnetic component of interaction. The electrostatic understanding of magnetic force is not well defined in physics in the process of electrodynamic. Cells use electrodynamic interaction within the cell are being studied as the basis and using the cell to measure and define electrodynamic interaction with the system that is biological a call. Specifically DNA thought the electronic interaction interactions. It appears infrared spectrum holds promises to help in revel these mechanisms. The promise of understanding or merely evaluation of electrodynamic interaction holds great promise to science with the greatest medical implication to understand genomic responses in cells. Understanding how the DNA interacts within a cell dynamic transition are known to take place and these are regulated thought electrodynamic interaction.

The method for increasing commodity rate of breeding black shrimps in the invention includes selecting excellent seeds, postponing the time of shrimp postlarvae stocking properly and feeding some chubs, bighead carps, etc., controlling the coverage rate of float grass and the transparency of water, and making use of calces. When in the metaphase of breeding black shrimps, more particularly, when the black shrimps are in the propagation season of Autumn, the area of float grass is controlled to be 60-70% of the water area, the transparency is controlled to be 40-50cm. In the propagation season, when the color of oogenesis of shrimps becomes grey and transparent, or flea-shape larva is hatched out, calces of 10-20g per stere is used for spraying the whole pool within five days, then the method is used every week and is totally used for 7-10 times. By increasing the measures of controlling the coverage rate of float grass and the transparency of water and making use of calces, neoteny of black shrimps and over propagation are controlled, the growth of grown shrimps is promoted, and the commodity rate of black shrimps can reach 90% at the end of the year. The method of breeding black shrimps has the advantages of easy and convenient operation as well as easy popularization and use, etc., which is a good method for effectively increasing commodity rate of breeding black shrimps.

The invention provides an excised mutagenesis tetraploid method of water melon and ploidy early stage certification technique, wherein dinitro toluene herbicide (DNH) is employed to substitute the conventional colchicines as inducer, whose function is to suppress the Mitosis in the metaphase of cell division through the mechanism of interfering spindle, so as to double the tissue cell chromosome. The method has the advantages of increased inducement success rate and substantially shortened time required for inducement.

The invention provides a multicolor fluorescence in situ hybridization (MFISH) method for quickly analyzing and identifying alien chromosome of wheat. The method comprises the following steps: (1) preprocessing actively dividing tissues in meristematic zone of wheat root tip by N2O and conducting enzymolysis on the actively dividing tissues to obtain a metaphase specimen with clear images and uniform distribution of chromosomes; (2) marking a fluorescent probe of fundamental genome of wheat by nick translation method; (3) conducting fluorescence in situ hybridization on chromosomes at mitosis metaphase obtained in step (1) by using the constructed fluorescent probe in step (2); and (4) analyzing the fluorescence in situ hybridization results obtained in step (3). According to the method provided by the invention, not only can alien chromosomes and chromosome segments in distant hybrid wheat be identified, but also alien chromosomes and chromosome segments of distant hybrids of other crops can be identified.

The invention discloses a method for extracting triterpene substances from inonotus obliquus by use of quorum sensing molecules induction, wherein the method comprises the following steps: adding activated inonotus obliquus CFCC 83414 into a liquid medium, and performing fermentation culture for 8-14 days at 24-26 DEG C; adding quorum sensing molecules in the 5th-8th days of the fermentation; after the fermentation, separating thalli and fermentation liquid; performing aftertreatment to separate and purify triterpene substances from the thalli and fermentation liquid. According to the method disclosed by the invention, quorum sensing molecules are added in the fermentation metaphase to stimulate growth of inonotus obliquus; the method does not need introduction of a gene engineering strainor additional enzyme, is simple in aftertreatment, and can effectively increase the yield of triterpene substances in inonotus obliquus.

The invention discloses a breeding method for a sow in a reproduction period. The method comprises the following steps: feeding in a reserve period, feeding in a pregnancy period, and feeding in a lactation period. According to the invention, directed at actual states of the sow in all stages of the reproduction period, targeted feeding with a feed with different components and proportions is economic, scientific and reasonable, and fully meets the requirements of the sow for nutrients in all stages at the same time, so the sow can remain a good growth state from oestrum to piglet delactation, i.e., the sow in an oestrous period has the advantages of well-developed, high-quality and numerous ova, high pregnancy rate, high survival rate of fertilized ova, high survival rate of early embryos in the early stage of mating, good developmental state of metaphase embryos, and high survival rates of the piglets after birth and in the lactation period; thus, the marketing rate of commercial pigs is fundamentally increased, and pig-raising efficiency is improved.

The invention discloses a fertilization method under the condition of drip irrigation cultivation under corn film, which belongs to the field of agricultural planting technology. The method is: mechanical fertilization, the fertilizer applicator is set with three fertilization ports, three rows of fertilizer are applied on each large ridge, and the middle row of fertilizer is applied to the ridge In the middle, the distance between the fertilization openings on the outermost two sides is 50-55cm; apply 60% fertilizer in the middle, and 20% fertilizer on each side; apply 15-17cm deep in the middle, and 8-10cm deep on both sides place. The beneficial effects are as follows: 1. Cross-layer fertilization. In the early stage of corn growth, its root system is short and can absorb shallow fertilizer, while in the middle and late stages of corn growth, its root system is longer and can absorb deep fertilizer; 2. Fertilize on both sides. There are fertilizers on both sides of the corn plant, and the closest distance to the fertilization belt is 5cm, which fully exerts the absorption of the fertilizer by the whole plant and improves the utilization rate of the fertilizer; 3. Fertilize on demand. Corn needs more fertilizer at the jointing and booting stage. At this time, the root system is longer and can absorb deep-level fertilizers, and the deep-level fertilizers account for a large proportion, which can just meet the needs of corn for fertilizers.

The invention relates to a chromosome karyotype analysis system which comprises the following steps: (1) designing a filtering algorithm and a segmentation algorithm to filter and remove impurities from a human metaphase cell image, and extracting a staining monomer; and (2) designing an identification algorithm and a correction algorithm, and identifying and pairing the extracted chromosomes so as to generate a karyotype map. According to the invention, the karyotype analysis method is combined with image processing, machine learning and other technologies, a reliable chromosome karyotype automatic analysis system is developed, automation and intelligence of chromosome karyotype analysis are realized, and the efficiency and the accuracy of chromosome karyotype classification are integrally improved.

The invention discloses corneal metaphase preserving liquid containing recombinant human serum albumin and a preparation method thereof. The corneal metaphase preserving liquid containing recombinanthuman serum albumin contains glutamine, calcium pantothenate, choline chloride, folic acid, inositol, nicotinamide, pyridoxal.HCl, riboflavin, thiamine.HCl, KCl, NaCl, NaH2PO4.2H2O, glucose, dextran 40, sodium pyruvate, vitamin C, chondroitin sulphate sodium, sodium hyaluronate, non-animal recombinant human serum albumin, gentamicin sulfate, sodium bicarbonate, piperazine-1-erhanesulfonic acid, phenol red indicator, and water for injection. The non-animal recombinant human serum albumin is added to replace serum to improve corneal endothelial cell survival rate; and then the non-animal recombinant human serum albumin cooperates with a compound system of the chondroitin sulphate sodium, the sodium hyaluronate and the dextran 40, the survival rate of the corneal endothelial cell is greatly ensured.

The invention discloses a method for identifying all chromosomes of poplar by using oligonucleotide probes. The method comprises the following steps: obtaining a specific oligonucleotide sequence of each chromosome through bioinformatics analysis according to genome information of poplar tomentosa; synthesizing an oligonucleotide library corresponding to chromosome 1-19 of poplar; marking the oligonucleotide probes by using biotin or digoxin; identifying the chromosomes of the poplar by using a fluorescence in-situ hybridization technology; and repeatedly carrying out fluorescence in-situ hybridization for 7 times on the same metaphase splitting phase by using the oligonucleotide probes of up to three chromosomes simultaneously in each cycle of hybridization, so that 19 pairs of chromosomes of poplar species can be accurately identified.The method has the advantages of good universality in poplar species, good experimental repeatability and high resolution.The methoddisclosed by the invention provides a novel method for accurately identifying each chromosome of the poplar species, and lays a solid working foundation for molecular cytogenetics research of poplar.

The invention discloses a medicament for rapidly treating burns and scalds. The finished product of the medicament is mainly prepared by fully mixing three components, namely ectotherm oil and fat, a centipede soaking solution and a beewax soaking solution, and the medicament belongs to the technical field of traditional Chinese medicines. Further, the prepared finished product is placed under a low-temperature condition of 4DEG C and is stood for 24 hours; then supernate is separated to obtain two types of products, namely paste products and liquid products. The medicament disclosed by the invention has the efficacy of resisting bacteria, diminishing inflammation, relieving swelling and pain, clearing away heat and toxic materials, removing necrotic tissue and promoting fresh blood production, drawing out toxin and promoting tissue regeneration, and the like, can be used for rapidly treating scales and burns caused by various reasons, does not need a desinfection chamber, can be applied any time and any place, and is suitable for popularization in vast rural areas and onsite rescue; according to the medicament, severe pains at an initial stage brought by the burns and scalds can be rapidly relieved, and infection under scabs in a metaphase is effectively prevented; the medicament is short in treatment cycle, good in effect, high in cure rate and free of scars after cure.

The invention provides an efficient sugarcane or sugarcane related species stem tip chromosome flaking method, and belongs to the technical field of cell biology. The invention provides an efficient sugarcane stem tip chromosome flaking method aiming at solving the problems that sugarcane chromosomes are large in number and small in form and ideal division phase metaphase cells are difficult to obtain through root tip flaking. Chromosome flaking is carried out by utilizing stem tip meristematic region tissues of sugarcane and sugarcane related species in a vigorous growth period, the method has the advantages of convenient material taking, large meristematic area tissue sample size, vigorous division, many metaphase cells and clear and dispersed chromosome structure, and the technical keypoints of material selection, pretreatment, fixation, dissociation dyeing, flaking and chromosome morphology microscopic observation are disclosed. The method is simple and convenient, accurate and reliable in result, good in repeatability, easy to operate and short in experiment period and improves the sugarcane chromosome genome analysis efficiency. Technical support is provided for sugarcane chromosome karyotype research, germplasm resource classification identification and protection utilization.

The invention relates to a preparation method of a turbellarian worm chromosome specimen. The preparation method of the turbellarian worm chromosome specimen comprises the steps of material preparation, pretreatment, regenerated tissue culture, tissue smashing, centrifugal suspending, colchicine blockage, KCl low permeability treatment, centrifugal suspending and dripping, dyeing, baking and sheet sealing. By adopting the preparation method, regenerated tissues and metaphase cells are fixed and treated fully, the chromosome preparation background is clear, cell dispersion is high, good cell scattering is realized, the number of metakinesis phases is large, and karyotype analysis is facilitated.

The invention discloses a cell telescoping structure model induced through mitosis retarding and a preparation method thereof. The method includes: retarding the mitosis metaphase through chemical drug or a genetics method to obtain a CIC cell model. The CIC cell model is induced through targeted retarding of mitosis. Building of the cell model is not limited in treatment mode and can be realizedno matter through the chemical drug or through the genetics method, and a stable and reliable model is provided for studying formation mechanism and physiological and pathological significance of CIC.Mitosis retarding induced CIC structure can be realized in normal epithelial cells, and the CIC cell model can be built in tumor cells. The CIC cell model can be built by using tumor targeting drug for targeted retarding of the mitosis metaphase, it shows that the model can serve as an in-vitro model for tumor therapy research.

The invention discloses a comprehensive control method for a wheat cyst nematode. The method includes the following steps: a, a wheat breed of Wenmai 4 or Taikong 6 that resists diseases is adopted; b, a nematocide ethoprophos is adopted during a seeding period and the processing method is to broadcast the ethoprophos with a dosage of 15 to 45Kg/hm<2> before plowing during a wheat seeding period and then turn over thereof into the soil; c, late broadcasting is adopted and the broadcasting period can be late according to the actual situation of the area for the early broadcasted wheat has a heavier disease; d, timely irrigating: the seedling stage, the overwintering as well as the reviving and jointing stage of wheat are three key stages which can be irrigated in time during the prophase and the early time of the metaphase of wheat growing. As adopting the method and steps, the invention has strong pertinence and good effect. Due to field experiments, the invention has no harm on wheat growing and the comprehensive control measure can ensure the control effect of wheat cyst nematode to reach about 90 percent.

The invention belongs to the field of molecular cytogenetics and particularly relates to a method for identifying D genome and D sub-genome complete chromosome. The method comprises the following step: by taking a sequence as shown in SEQ ID NO.1 as a probe to perform fluorescence in-situ hybridization on the DNA (deoxyribonucleic acid) of a cotton genome. The sequence is taken as the probe, and chromosomes in the mitosis metaphase of the upland cotton, the asiatic cotton and the Raymond's cotton are taken as target DNA to perform fluorescence in-situ hybridization, and the results indicate that obvious hybrid signals are shown on the D subgenome chromosome of the upland cotton and the D genome chromosome of the Raymond's cotton, the signals are distributed on all chromosomes, and no obvious signals are shown in the A subgenome chromosome of the upland cotton and the A genome chromosome of the asiatic cotton. The sequence adopted by the method disclosed by the invention can be used for quickly identifying the cotton D genome and D sub-genome complete chromosome by virtue of a FISH (fluorescence in situ hybridization) method.

The invention belongs to the technical field of plant identification and discloses a tabletting method of eucalyptus chromosome.The tabletting method includes following steps: taking materials, pretreating, fixing, dissociating, dyeing, tabletting and performing microscopic examination.In the tabletting method, oryzalin is adopted as a pretreatment agent, so that cytotoxic effect is reduced obviously when cell mitosis is blocked; cells can be in normal mitosis, so that during fixing, number of accumulated metaphase cells is large, chromosome tabletting success probability is increased, and stability is improved.Oryzalin is low in cost and far lower than colchicine in toxicity; in the tabletting process, environment pollution can be reduced and degree and risk of causing damage to people are lowered.

The invention discloses a method for preparing a pelochelys bibroni chromosome specimen by using peripheral blood cells, and belongs to the field of cytogenetics. Peripheral blood of a pelochelys bibroni is collected, and a pelochelys bibroni chromosome metaphase specimen which is convenient to observe and clear in image is prepared through the steps of blood cell culture, colchicine treatment, staining and slide preparation and the like. According to the method of the invention, the problem of large damage to animals in an existing aquatic animal chromosome specimen preparation method is solved, non-damage sampling is realized, only a small amount of peripheral blood of the sample needs to be collected, and the influence on the living state of the animals is small. The slide preparation method is simple and rapid; the metaphase of the pelochelys bibroni chromosome can be obtained 72 hours after blood sampling, observation is facilitated, and a beneficial tool is provided for further developing pelochelys bibroni genome and evolution related research and realizing more sufficient protection of the pelochelys bibroni .

The invention belongs to the field of bio-medicine and discloses the expression of recombinant human midkine (rh-Midkine) and the preparation and the application of the monoclonal antibody thereof. The invention is characterized in that the prokaryotic expression of midkine (Midkine, MK) is achieved by Escherichia coli, and the obtained recombinant protein 30P-MK has a bioactivity for promoting cell proliferation so as to provide foundation for the research of MK in the fields of tumor, cell biology, nervous system, etc. Anti-human MK monoclonal antibody hybridoma can be prepared by immunizing Balb/c mouse by 30P-MK protein after purifying the 30P-MK protein, fusing spleen cells and SP2/0 cells and sieving, and the hybridoma can secrete IgG1 monoclonal antibody. It is verified by Western-blotting of midkine in human tumor tissue sample and immune histochemistry that the monoclonal antibody has high specificity and low background and can be used for diagnosis kit for clinically and experimentally determining the expression level of MK in tumor tissue and blood serum, thus establishing the foundation for target therapy of tumor by using MK monoclonal antibody.