16 results about "Metaphase" patented technology

The invention discloses a slide preparation method for rapidly obtaining root tip chromosomes of Shenghua and application, and belongs to the technical field of chromosome slide preparation. The flaking method comprises the following steps: carrying out cuttage water culture on node vines at the overground part of the ginseng and the sweet potatoes, and inducing to generate adventitious roots; the root tips of the adventitious roots are taken and placed in a pretreatment solution to be pretreated; the pretreated root tips are placed in a fixing solution to be fixed; sequentially carrying out acidolysis and enzymolysis on the fixed root tips; taking root tip meristematic zone tissues after acidolysis and enzymolysis, placing the root tip meristematic zone tissues on a glass slide, dyeing, tabletting, and placing on a heating plate for heating; according to the flaking method disclosed by the invention, the division phase metaphase cells with clean flaking background, clear chromosome morphological structure, obvious chromosome overflow mark and good dispersion can be rapidly obtained, the chromosome number statistics and karyotype analysis are convenient, and the flaking method has important significance on ploidy identification, variety identification, genetic relationship identification and the like of the sweet potatoes.

The invention belongs to the technical field of biology, and particularly relates to a detection method of a tumor serological marker. The application of the reagent for detecting the concentration of the sGRP78 protein in the serum of the breast cancer patient in preparation of a product for evaluating the curative effect of the breast cancer patient after neoadjuvant therapy is characterized in that the number of neoadjuvant therapy is four. In order to overcome the defects of dynamic monitoring deficiency, invasiveness, high cost and insufficient serum marker sensitivity in the existing neoadjuvant therapy metaphase evaluation of the breast cancer, the invention provides a detection kit, namely, the serum marker sGRP78 is monitored in the neoadjuvant therapy metaphase evaluation of the breast cancer, and the sensitivity of the serum marker sGRP78 in the neoadjuvant therapy metaphase evaluation of the breast cancer is improved. Therefore, a noninvasive, low-cost, high-sensitivity and high-specificity neoadjuvant therapy metaphase evaluation index is achieved.

The invention relates to the technical field of chromosome recognition and segmentation, in particular to a chromosome recognition and segmentation method based on an attention mechanism and small target detection, which comprises the following steps: acquiring an image containing a metaphase of a chromosome, and preprocessing the image; secondly, on the basis of a YOLOv8-seg framework, an asymptotic feature pyramid network is introduced, asymptotic fusion is carried out on feature maps of different levels, a semantic gap caused by fusion between non-adjacent layers in a traditional network is avoided, and the small target chromosome detection precision is improved; on the basis of an output layer of the asymptotic feature pyramid network, a small target detection layer is introduced, a detection result of a small target is output through convolution operation, activation and feature fusion, and the introduction of the small target detection layer enables the number of convolution times to be small and the size of a feature map of an image to be large, so that small target recognition is more facilitated; and finally, by taking the YOLOv8-seg framework as a baseline, introducing an extrusion encouraging attention mechanism network behind the final convolutional layer of the neural network.

The invention provides an improved euchiloglanis kishinouyei chromosome preparation method, which comprises the following steps: gradually raising the temperature and injecting PHA twice: in the euchiloglanis kishinouyei temporary culture process, raising the water temperature from 18 DEG C to 20 DEG C within 12 hours, injecting a first needle of PHA, raising the temperature to 22.5-23 DEG C after 12 hours, injecting a second needle of PHA, and keeping the water temperature at 22.5-23 DEG C unchanged; after the PHA is injected for the second time for 3 hours, colchicine is injected; sampling: washing the head and kidney in normal saline, and collecting cells; 0.5% of KCl is used for hypotonic treatment; fixing with a Carnoy's fixing solution, and centrifuging; dropping by a cold dropping method and naturally drying; and carrying out Giemsa dyeing and flaking. According to the method, the euchiloglanis kishinouyei somatic cell proliferation is promoted through heating and twice PHA injection, so that the metaphase division phase is increased, the form is clear and dispersed, and the karyotype quality and the preparation success rate of the chromosome are remarkably improved.

The invention belongs to the technical field of karyotype analysis of chromosomes, and particularly relates to a cell pretreatment solution for karyotype analysis and preparation of chromosomes and application of the cell pretreatment solution. The cell pretreatment solution comprises the following components: 15 mmol / L Hepes buffer solution with the pH value of 7.2-7.6, 0.08-1.2 [mu] g / mL of vinblastine, 0.1-0.5 v / v% of Triton X-100 and 1-2 mmol / L of EDTA (Ethylene Diamine Tetraacetic Acid), and is a cell pretreatment solution with low toxicity, better dyeing effect and good cell type universality. When the cell pretreatment liquid is used for preparing a chromosome specimen, the preparation time is remarkably shortened, not only can more metaphase cell division phases be obtained, but also the proportion of the division phases capable of being used for chromosome analysis is greater than 83%, and the efficiency of karyotype analysis is remarkably improved. Meanwhile, the prepared chromosome specimen is clear in structure number, good in form, high in dispersity and few in background impurities, and the accuracy of chromosome karyotype analysis is remarkably improved.

The present invention belongs to the technical field of chromosome karyotype analysis, and specifically discloses a chromosome karyotype analysis method and system based on deep learning. The method includes the following steps: S1, obtaining a metaphase image of a chromosome and performing preprocessing; S2, extracting a feature F from the image; S3, clipping the feature F using multiple suggestion frames to obtain features F1 to Fn; S4, removing the common features of features F1 to Fn to obtain modified features f1 to fn, and performing classification to obtain a predicted classification result, using loss functions IG Loss and CE Loss for joint supervision; S5, fine-tuning the suggestion frame and using CE Loss to supervise the fine-tuning result; S6, using binary crossover loss (BCE Loss) to supervise the predicted classification result to obtain the chromosome karyotype analysis result. Using this technical solution, chromosome karyotype analysis is completed using deep learning and segmentation frames, and the chromosome classification effect is targetedly optimized according to the characteristics of the chromosome, thereby improving overall performance.

The invention provides a preparation method of lycoris plant root tip chromosomes, and belongs to the technical field of biology. The preparation method of the lycoris plant root tip chromosome comprises the steps of root tip induction culture, root tip collection and pretreatment, enzymolysis treatment, chromosome flaking, microscopic observation and the like. A normal-temperature and low-temperature combined 8-hydroxyquinoline double-stage pretreatment mode is adopted for newborn root tips, and a compound enzymatic hydrolysate and a buffer solution pre-preservation system with an optimized ratio are combined, so that the metaphase division cell proportion and the chromosome image quality are effectively improved. The method has the advantages of simplicity and convenience in operation, high efficiency, strong repeatability and the like, is suitable for preparing chromosomes of different types of lycoris radiate and hybrid materials thereof, and can also be popularized and applied to cytogenetics research of other bulb ornamental plants.

The invention provides a pretreatment method for improving the cell cryopreservation-resuscitation success rate in the metaphase of differentiation of human embryonic stem cells and application, and the pretreatment method comprises the following steps: culturing the human embryonic stem cells in a composite culture system for a period of time before kidney organoid induced differentiation begins; wherein the composite culture system comprises a basic culture medium and an additive, and the additive comprises a PD0332991 reagent. According to the method, the human embryonic stem cells are pretreated before induced differentiation starts, and PD0332991 improves the tolerance of differentiated metaphase cells to cryopreservation stress on the premise of not influencing the differentiation potential of hESC through a dual mechanism of cell cycle regulation and stress defense enhancement, so that the cryopreservation-recovery survival rate of Day4 differentiated metaphase cells can be obviously improved, and the survival rate of the Day4 differentiated metaphase cells is improved. The vast majority of resuscitation cells are ensured to be continuously differentiated into kidney organs with complete structures and normal marker expression.

This application relates to a chromosome image processing method, apparatus, computer device, computer-readable storage medium, and computer program product. The method includes: acquiring an input image containing multiple chromosomes in metaphase; extracting individual chromosome images from the input image; performing feature analysis on each chromosome based on its image to obtain multidimensional chromosome feature information; and determining the phase quality assessment result of the input image based on statistical information corresponding to the multidimensional chromosome feature information. This method can improve the accuracy of chromosome image processing.

The invention discloses a metaphase division cell optimization method and system for chromosome aberration detection, and relates to the technical field of information processing, and the method comprises the following steps: S1, carrying out the grading labeling of an obtained metaphase division chromosome image, and constructing an image grading data set; s2, training the artificial intelligence model through the image grading data set to obtain a preliminary screening grading model; s3, grading and screening the real-time chromosome image data through the preliminary screening and grading model to obtain a to-be-selected image data set; s4, extracting morphological features and corresponding weight parameters of each chromosome image in the to-be-selected image data set to construct a basic scoring function; s5, the basic scoring function is combined with an AI confidence coefficient weighting mechanism to determine a scoring sequence of each level of image; and arranging the scoring sequences in a descending order and outputting target images in sequence. The problems of low screening efficiency, poor result consistency and one-sided evaluation dimension in the prior art are solved, and the stability and reliability of the screening result are improved.

The invention provides a slide preparation method for observing mammalian bone marrow cell chromosome aberration, and belongs to the technical field of biological detection. The slide specimen preparation method provided by the invention can meet the observation requirement of a standard operation procedure, the prepared slide specimen is superior in quality, the experimental operation period is shortened, and the slide specimen preparation method has wide application potential. The specimen prepared by the method has more metaphase division phase cells, the chromosome morphology is kept stable, and the slide preparation effect is remarkable. By using sodium citrate with an anticoagulation characteristic, the interference of peripheral blood mixing on the slide preparation process is effectively reduced, and the selective collection of metaphase division phase cells is realized, so that the concentration of target cells is improved. Besides, the method avoids the chromosome expansion phenomenon, so that the chromosome morphology is relatively thick in the observation process, and chromosome loss caused by serious rupture of a cell membrane is not easily caused.

The embodiment of the invention discloses a chromosome specimen processing method and device and electronic equipment, and the method comprises the steps: responding to a first triggering operation, and synchronizing at least one split phase scanning image of a target chromosome specimen scanned by a first system to a second system based on target configuration information; for the at least one split phase scanning image, based on a pre-trained target cutting arrangement model, determining a chromosome karyotype graph corresponding to the split phase scanning image; the chromosome analysis result of the target object to which the chromosome specimen belongs is determined based on the chromosome karyotype graph corresponding to the at least one split phase scanning image, chromosome data transmission of the non-matched system can be automatically carried out according to the configuration information, the data transmission efficiency of the non-matched system is improved, the chromosome karyotype analysis time is shortened, and the analysis efficiency of the chromosome karyotype is improved. And the accuracy and the working efficiency of chromosome scanning analysis are improved.

The invention discloses a method for preparing tobacco metaphase chromosomes by utilizing young tobacco plants and application, and belongs to the technical field of preparation of tobacco chromosome specimens. The method comprises the following steps: germinating tobacco seeds at room temperature for 5-7 days, stripping seed shells when the seeds germinate and radicles grow to about 2 mm, sequentially performing N2O treatment on seedlings with radicles, fixing with a stationary liquid, performing enzymolysis in an enzymatic hydrolysate, lightly grinding plant tissues by using a sterile grinding rod until cells are dispersed, centrifuging, airing, adding glacial acetic acid, performing uniform vortex mixing, and performing freeze drying to obtain the tobacco seed suspension. And dripping a small amount of suspension on a clean glass slide to prepare the tobacco metaphase chromosome specimen. According to the method, materials are easy to obtain, the period is extremely short, operation is safe, the chromosome quality is high, and a reliable and efficient standardized scheme is provided for tobacco cytogenetics, molecular cytology and breeding research.

The invention discloses a construction method and application of a DFNB1 type deafness mouse model with GJB2 gene frameshift mutation. The construction method comprises the following steps: (1) designing two sgRNA plasmids on a No.2 exon of a mouse Gjb2 gene; (2) screening out mouse embryonic stem cells of which the Gjb2 gene carries a c.299300delAT mutation site; (3) forming a recombinant embryo, transplanting the recombinant embryo into the uterus of the pregnant female mouse, and waiting for pregnancy and farrowing to obtain an F0-generation mouse; and (4) screening the F0 generation mice in the step (3) to obtain the mouse model carrying the c.299300delAT homozygous point mutation of the GJB2 gene. The method has the advantages that the problem of mouse embryo metaphase energy supply disorder lethal caused by Gjb2 homozygous mutation is solved, the embryo survival rate is increased, and the bottleneck of embryo lethal in model construction of a traditional gene editing technology is broken through.

The invention belongs to the technical field of cell culture, and discloses a bone marrow cell culture medium and a preparation method thereof, and a method for in-vitro culture of bone marrow cells, the bone marrow cell culture medium is composed of a basic culture medium, streptomycin, gentamicin, a platelet lysis buffer, and glutamate enzyme; the final concentration of streptomycin is 50-100 [mu] g / mL, the final concentration of gentamicin is 20-50 [mu] g / mL, the final concentration of platelet lysate is 5-15% (v / v), and the final concentration of glutamate enzyme is 0.1-1 mmol / L. The bone marrow cell culture medium can efficiently support in-vitro proliferation of bone marrow cells, through specific combination and appropriate concentration control of the culture medium, the medium-term cell acquisition amount can be remarkably increased, and medium-term cell chromosomes are complete in morphology and good in dispersity; the standard requirements of clinical bone marrow cell chromosome karyotype analysis, blood disease diagnosis and cell genetics experiments in the scientific research field can be met.

The present disclosure provides methods and compositions for analyzing enriched populations of cells or target chromosomes, for example cells in metaphase and methods and compositions for two-dimensional spatial arrangement of cells and chromosomes. Furthermore, methods are disclosed for the detection of structural variations and / or repair events in chromosomes by labeling of single-stranded chromatids with probes, which in illustrative embodiments are of different colors. The hybridization pattern of the labeled probes produces a spectral pattern that provides high-resolution detection of structural variations and / or repair events, which for example can facilitate distinction of benign structural variations from deleterious structural variations. Further, the spectral pattern provides information regarding complex structural variations where more than one rearrangement of chromosomal segments may have occurred. Spectral information can be used to generate data tables upon which nodal analysis can be applied to identify structural features of interest.