7 results about "Lycoris" patented technology

The invention discloses a method for preparing an in-vitro insect repellent spray for pets by using a lycoris plant extract, which comprises the following steps: S1, raw material pretreatment: cleaning, drying and crushing a lycoris raw material to obtain lycoris powder; s2, extracting the lycoris powder by using an ethanol reflux extraction method to obtain an extraction filtrate; and S3, firstly concentrating the lycoris radiata extract filtrate to obtain a thick lycoris radiata extract concentrate, then performing impurity removal and purification on the concentrate, and after purification is completed, performing concentration again to obtain a pure lycoris radiata extract. The spray has the advantage of being good in parasite expelling effect, and the problems that in the preparation process of an existing pet in-vitro parasite expelling spray, multiple raw materials cannot be evenly mixed conveniently, ingredient distribution is prone to being uneven, and the in-vitro parasite expelling effect on pets is affected are solved.

The application provides a LiCOMT gene for regulating synthesis of lycoris kingianus glycosides and application thereof, belongs to the technical field of plant genetic engineering, and the nucleotide sequence of the LiCOMT gene is shown as SEQ ID NO. 7, and the amino acid sequence of the encoded protein is shown as SEQ ID NO. 8; the application screens out a candidate gene LiCOMT significantly related to lycoris kingianus glycoside accumulation from lily bulb development period transcriptome data through weighted gene co-expression network analysis (WGCNA), and confirms that silencing the LiCOMT gene can reduce the content of lycoris kingianus glycoside A, lycoris kingianus glycoside B and lycoris kingianus glycoside F in lily bulbs by about 60%, 42% and 43% respectively by using a virus-induced gene silencing (VIGS) technique; and the application provides important gene resources and theoretical basis for lily secondary metabolism regulation and molecular breeding.

This invention belongs to the field of plant propagation and cultivation technology, specifically relating to a tissue culture medium and method for the regeneration of Lycoris radiata. This invention provides a tissue culture medium for Lycoris radiata regeneration, comprising a callus induction medium, a callus proliferation medium, and a callus differentiation medium. In the tissue culture medium and method provided by this invention, single scales are selected as explants, and modified MS medium and B5 medium are used as basal media, with appropriate concentrations of 6-BA, NAA, carbon nanotubes, and acid-hydrolyzed casein added. Combined with suitable temperature and light, this facilitates the induction of Lycoris radiata callus proliferation and differentiation, increases the propagation coefficient of Lycoris radiata, and yields a large number of uniform Lycoris radiata tissue culture seedlings.

The invention provides a Chinese ladiestresses root extract as well as a preparation method, a related product and application thereof, and belongs to the technical field of traditional Chinese medicine extraction. The preparation method comprises the following steps: mixing pedicularis chinensis and ketone, and performing reflux leaching and separation to obtain a leaching solution and medicine residues; recovering the solvent from the leaching solution to obtain a Chinese ladiestresses root extract; mixing the medicine residue with water, decocting, centrifuging the decoction, reserving the supernatant, and carrying out alcohol precipitation to obtain crude polysaccharide; reacting the crude polysaccharide with sodium periodate to obtain oxidized paniculate helminthostachys rhizome polysaccharide; and mixing 5-15 parts by weight of the paniculate helichrysum herb extract, 10-30 parts by weight of the oxidized paniculate helichrysum herb polysaccharide and 100 parts by weight of water, and carrying out ultrasonic dispersion and drying to obtain the paniculate helichrysum herb extract. The spiranthes sinensis extract obtained by the method can form a submicron-level particle structure, and can better reduce the foot swelling score of rheumatoid arthritis mice, improve the blood marker index of model mice and reduce the occurrence of hemolysis side effects.

The invention relates to the technical field of adhesives, and discloses an environment-friendly lycoris starch-vinyl acetate adhesive and a preparation method thereof.The preparation method comprises the steps that water is added into a reaction kettle and stirred, a defoaming agent and polyvinyl alcohol are added, the mixture is heated to 85 DEG C and then self-heated to 90 DEG C, and heat preservation is conducted; cooling to 83 DEG C, adding an emulsifier and part of an initiator, sealing and starting a condenser; slowly dropwise adding vinyl acetate, maintaining the temperature at 77-80 DEG C, and supplementing the initiator every 30 minutes; after dropwise adding, adding the rest initiator, heating to 86 DEG C, and preserving heat; cooling to 83 DEG C, slowly adding lycoris starch paste, and gelatinizing for 30 minutes; and cooling to 65 DEG C, adding the plasticizer dibutyl phthalate, cooling to 50 DEG C, adding the preservative, stirring for 30 minutes, filtering, and packaging to obtain the finished product adhesive. The lycoris starch is introduced into a polyvinyl alcohol-vinyl acetate system, so that the prepared adhesive has the characteristics of high bonding strength, stable viscosity, slight smell and environmental friendliness.

The invention belongs to the technical field of plant preservation, and particularly relates to a compound preservative for lycoris plant fresh cut flowers as well as a preparation method and application of the compound preservative. The compound preservative is prepared from the following effective components in mass concentration: 150 to 200mg / L of citric acid, 15 to 20g / L of cane sugar, 2 to 3g / L of calcium chloride, 1 to 2g / L of chitosan, 1 to 2ppm of nano-silver colloid and 3 to 5mg / L of gibberellin. According to the invention, the nano-silver colloid, the chitosan and the hormone are combined for post-harvest treatment of lycoris plant cut flowers for the first time; four functions of antibiosis, cell energy stabilization, nutrition supply and hormone regulation are integrated, a multi-factor synergistic mechanism is established, and then the postharvest preservation effect of the lycoris plant cut flowers is remarkably improved. Results of the embodiment show that the composite preservative can prolong the life of the lycoris plant cut flower vase to 6-8 days, the phenomena that the base part of the flower stem is curled and the petals are premature senility are obviously reduced, and the good ornamental value is maintained.

The invention relates to an allium tuberosum egg composition for treating haemorrhoids as well as a preparation method and application thereof, and belongs to the field of traditional Chinese medicine preparations. According to the composition, a pharmacological structure with a slow-release effect is formed through synergistic compatibility of fresh Chinese chive leaf lotus products (2 cm of rhizomes are removed from 30-50 cm sections, and the Chinese chive leaf lotus sections are cut into small sections of 0.5-1.0 cm) treated by a specific process and fresh eggs (with shells of 50-60 g) by adopting a hot-pot cold-oil instantaneous frying process (180 + / -10 DEG C fire turning off and cake spreading, and braising in a half-cooked state for 3-5 minutes). In the preparation process, the design of a food-grade stainless steel pot (the inner diameter is larger than or equal to 30 cm) and a detachable anti-sticking layer is adopted, a finished product needs to be subjected to 6kGy irradiation sterilization within 4 hours, and the quality stability is ensured. The invention provides a natural and efficient new choice for treating haemorrhoids, and is especially suitable for stubborn cases with chemical medicine failure.