17 results about "Lycoris" patented technology

The invention discloses a method for preparing an in-vitro insect repellent spray for pets by using a lycoris plant extract, which comprises the following steps: S1, raw material pretreatment: cleaning, drying and crushing a lycoris raw material to obtain lycoris powder; s2, extracting the lycoris powder by using an ethanol reflux extraction method to obtain an extraction filtrate; and S3, firstly concentrating the lycoris radiata extract filtrate to obtain a thick lycoris radiata extract concentrate, then performing impurity removal and purification on the concentrate, and after purification is completed, performing concentration again to obtain a pure lycoris radiata extract. The spray has the advantage of being good in parasite expelling effect, and the problems that in the preparation process of an existing pet in-vitro parasite expelling spray, multiple raw materials cannot be evenly mixed conveniently, ingredient distribution is prone to being uneven, and the in-vitro parasite expelling effect on pets is affected are solved.

The application provides a LiCOMT gene for regulating synthesis of lycoris kingianus glycosides and application thereof, belongs to the technical field of plant genetic engineering, and the nucleotide sequence of the LiCOMT gene is shown as SEQ ID NO. 7, and the amino acid sequence of the encoded protein is shown as SEQ ID NO. 8; the application screens out a candidate gene LiCOMT significantly related to lycoris kingianus glycoside accumulation from lily bulb development period transcriptome data through weighted gene co-expression network analysis (WGCNA), and confirms that silencing the LiCOMT gene can reduce the content of lycoris kingianus glycoside A, lycoris kingianus glycoside B and lycoris kingianus glycoside F in lily bulbs by about 60%, 42% and 43% respectively by using a virus-induced gene silencing (VIGS) technique; and the application provides important gene resources and theoretical basis for lily secondary metabolism regulation and molecular breeding.

The present invention relates to the field of plant cultivation technology, discloses a method for postponing the flowering of Lycoris bulbs, comprising the following steps: S1, 6-9 months, 1-7 days before the Lycoris bulbs bud, the Lycoris bulbs are dug out and transplanted into a planting box for hypophysing, and a planting matrix is ​​filled in the planting box; S2, the planting box is placed in an indoor environment where a temperature of 34-36 DEG C and a relative air humidity of 80-90% are stored, and the storage is 7-60 days; S3, the temperature is adjusted to 23-25 ​​DEG C and the flowers are destroyed, and the relative air humidity is adjusted to 60-75%; S4, after budding, the flowers are transferred to an outdoor natural environment and grown to flowering. The present invention can postpone flowering for 7-60 days after processing the Lycoris bulbs, so that the flowering process can be delayed according to actual exhibition time arrangements, to meet the postponed viewing needs.

The invention provides application and method of geotrichum galactose yeast NBG001 in improving accumulation of alkaloids for lycoris radiata traditional Chinese medicine, and belongs to the technical field of agricultural production, the geotrichum galactose yeast NBG001 is preserved in China Center for Type Culture Collection on July 7, 2025, and the preservation number is CCTCC NO: M 20251547. By inducing the expression of signal molecules and key genes in the lycoris radiate, the simultaneous accumulation of galanthamine, lycorine and narcissus in the lycoris radiate is remarkably improved.

The invention relates to an oil-control moisturizing composition containing pleione bulbocodioides extract and application of the oil-control moisturizing composition. The composition is prepared from, by mass, 3-8 parts of pleione bulbocodioides extract, 1-5 parts of panax japonicus extract, 1-3 parts of sculellaria barbata extract and 1-2 parts of jasminum grandiflorum extract. The composition prepared by the invention is simple in components, all the components are natural plant sources, the safety is high, all the components have a mutual synergistic effect, and the oil control and moisturizing effects are remarkable.

The invention provides a preparation method of lycoris plant root tip chromosomes, and belongs to the technical field of biology. The preparation method of the lycoris plant root tip chromosome comprises the steps of root tip induction culture, root tip collection and pretreatment, enzymolysis treatment, chromosome flaking, microscopic observation and the like. A normal-temperature and low-temperature combined 8-hydroxyquinoline double-stage pretreatment mode is adopted for newborn root tips, and a compound enzymatic hydrolysate and a buffer solution pre-preservation system with an optimized ratio are combined, so that the metaphase division cell proportion and the chromosome image quality are effectively improved. The method has the advantages of simplicity and convenience in operation, high efficiency, strong repeatability and the like, is suitable for preparing chromosomes of different types of lycoris radiate and hybrid materials thereof, and can also be popularized and applied to cytogenetics research of other bulb ornamental plants.

The invention provides an ultralow-temperature preservation method of lycoris bulblets, and belongs to the technical field of plant tissue culture. The method comprises the following steps: 1, treating an explant; 2, establishing a tissue culture system; 3, subculturing and inducing the bulblets; 4, pre-culturing the bulblets; 5, cutting bulblets and fixing loading strips; 6, loading and vitrification treatment; 7, freezing, unfreezing and washing; and 8, recovering culture. The rapid tissue propagation and ultralow-temperature preservation method of the lycoris radiate is convenient and efficient, and has reference significance on ultralow-temperature preservation of the lycoris radiate and other bulb and perennial root plant genetic resources.

This invention belongs to the field of plant propagation and cultivation technology, specifically relating to a tissue culture medium and method for the regeneration of Lycoris radiata. This invention provides a tissue culture medium for Lycoris radiata regeneration, comprising a callus induction medium, a callus proliferation medium, and a callus differentiation medium. In the tissue culture medium and method provided by this invention, single scales are selected as explants, and modified MS medium and B5 medium are used as basal media, with appropriate concentrations of 6-BA, NAA, carbon nanotubes, and acid-hydrolyzed casein added. Combined with suitable temperature and light, this facilitates the induction of Lycoris radiata callus proliferation and differentiation, increases the propagation coefficient of Lycoris radiata, and yields a large number of uniform Lycoris radiata tissue culture seedlings.

The invention provides a Chinese ladiestresses root extract as well as a preparation method, a related product and application thereof, and belongs to the technical field of traditional Chinese medicine extraction. The preparation method comprises the following steps: mixing pedicularis chinensis and ketone, and performing reflux leaching and separation to obtain a leaching solution and medicine residues; recovering the solvent from the leaching solution to obtain a Chinese ladiestresses root extract; mixing the medicine residue with water, decocting, centrifuging the decoction, reserving the supernatant, and carrying out alcohol precipitation to obtain crude polysaccharide; reacting the crude polysaccharide with sodium periodate to obtain oxidized paniculate helminthostachys rhizome polysaccharide; and mixing 5-15 parts by weight of the paniculate helichrysum herb extract, 10-30 parts by weight of the oxidized paniculate helichrysum herb polysaccharide and 100 parts by weight of water, and carrying out ultrasonic dispersion and drying to obtain the paniculate helichrysum herb extract. The spiranthes sinensis extract obtained by the method can form a submicron-level particle structure, and can better reduce the foot swelling score of rheumatoid arthritis mice, improve the blood marker index of model mice and reduce the occurrence of hemolysis side effects.

The invention discloses a Penicillium beijeri and Purpureocillium lilacinum complex microbial inoculant and application thereof in preventing and treating green onion and garlic root rot, and relates to the technical field of microorganisms, and the technical scheme is characterized in that the Penicillium beijeri and Purpureocillium lilacinum complex microbial inoculant comprises Purpureocillium lilacinum LS003 and Penicillium beijeri LS004; according to the purpureocillium lilacinus LS003, the Latin name is Paecilomyces lilacinus, and the preservation number of the purpureocillium lilacinus LS003 is CGMCC (China General Microbiological Culture Collection Center) NO.41613; the Latin name of the penicillium bilaiae LS004 is Penicillium bilaiae, and the preservation number of the penicillium bilaiae LS004 is CGMCC (China General Microbiological Culture Collection Center) NO.41614. The disease-preventing and growth-promoting compound microbial agent provided by the invention has good phosphorus-dissolving and growth-promoting functions, and is a good microbial agent which can be used for efficiently promoting growth of lycoris crops.

The invention relates to the technical field of adhesives, and discloses an environment-friendly lycoris starch-vinyl acetate adhesive and a preparation method thereof.The preparation method comprises the steps that water is added into a reaction kettle and stirred, a defoaming agent and polyvinyl alcohol are added, the mixture is heated to 85 DEG C and then self-heated to 90 DEG C, and heat preservation is conducted; cooling to 83 DEG C, adding an emulsifier and part of an initiator, sealing and starting a condenser; slowly dropwise adding vinyl acetate, maintaining the temperature at 77-80 DEG C, and supplementing the initiator every 30 minutes; after dropwise adding, adding the rest initiator, heating to 86 DEG C, and preserving heat; cooling to 83 DEG C, slowly adding lycoris starch paste, and gelatinizing for 30 minutes; and cooling to 65 DEG C, adding the plasticizer dibutyl phthalate, cooling to 50 DEG C, adding the preservative, stirring for 30 minutes, filtering, and packaging to obtain the finished product adhesive. The lycoris starch is introduced into a polyvinyl alcohol-vinyl acetate system, so that the prepared adhesive has the characteristics of high bonding strength, stable viscosity, slight smell and environmental friendliness.

The invention discloses a method for preparing a pet disinfectant by using a lycoris plant extract, which comprises the following steps: S1, lycoris plant extraction: extracting alkaloid in lycoris plants to prepare an alkaloid solution; s2, antibacterial plant extraction: extracting antibacterial disinfection components in antibacterial plants to prepare a supplementary disinfectant; s3, preparing ingredients of the pet disinfectant, namely preparing a surfactant, a humectant, a pH regulator, a buffering agent and an antistatic agent as the ingredients of the pet disinfectant. The method has the advantages of high efficiency and automation, and solves the problems that the production efficiency of the pet disinfectant is low, the extraction effect is unstable, the production cost is high and the like due to the fact that the existing method for preparing the pet disinfectant from the plant extract is inconvenient to efficiently and automatically extract the plant extract in the using process. And the quality of a final product is influenced.

The invention belongs to the technical field of plant preservation, and particularly relates to a compound preservative for lycoris plant fresh cut flowers as well as a preparation method and application of the compound preservative. The compound preservative is prepared from the following effective components in mass concentration: 150 to 200mg / L of citric acid, 15 to 20g / L of cane sugar, 2 to 3g / L of calcium chloride, 1 to 2g / L of chitosan, 1 to 2ppm of nano-silver colloid and 3 to 5mg / L of gibberellin. According to the invention, the nano-silver colloid, the chitosan and the hormone are combined for post-harvest treatment of lycoris plant cut flowers for the first time; four functions of antibiosis, cell energy stabilization, nutrition supply and hormone regulation are integrated, a multi-factor synergistic mechanism is established, and then the postharvest preservation effect of the lycoris plant cut flowers is remarkably improved. Results of the embodiment show that the composite preservative can prolong the life of the lycoris plant cut flower vase to 6-8 days, the phenomena that the base part of the flower stem is curled and the petals are premature senility are obviously reduced, and the good ornamental value is maintained.

The invention relates to a pleione bulbocodioides extract preparation method, a pleione bulbocodioides extract product and application, the preparation method comprises the following steps: crushing pleione bulbocodioides, mixing the crushed pleione bulbocodioides with water, and carrying out ultrahigh pressure treatment to obtain a first mixture; carrying out subcritical extraction on the first mixture, and filtering to obtain an extracting solution; filtering the extracting solution through a nanofiltration membrane to obtain pleione bulbocodioides filtrate; and mixing the pleione bulbocodioides filtrate with a decolorizing agent, filtering, and drying the filtrate to obtain the pleione bulbocodioides decolorizing agent. The method disclosed by the invention is high in extraction efficiency, green and environment-friendly, the obtained extract is rich in active ingredients, can effectively activate the expression of silk polyprotein and paphiopetin, promote the repair of keratinocytes and inhibit sebaceous gland cells from secreting grease, and can maximize the barrier repair and oil control effects of the extract under a specific extraction process.

The use of extracts from plants of the genera *Lycoris* and *Zephyranthes* in the control of rice blast belongs to the field of plant-derived fungicide technology. This paper discloses the uses of extracts from seven *Lycoris* species and one *Zephyranthes* species in the control of rice blast. These extracts can effectively inhibit the mycelial growth and spore germination of *Bacillus oryzae*, and have a good control effect on rice blast. The use of these extracts can delay the development of fungicide resistance in *Bacillus oryzae*.

The invention relates to an allium tuberosum egg composition for treating haemorrhoids as well as a preparation method and application thereof, and belongs to the field of traditional Chinese medicine preparations. According to the composition, a pharmacological structure with a slow-release effect is formed through synergistic compatibility of fresh Chinese chive leaf lotus products (2 cm of rhizomes are removed from 30-50 cm sections, and the Chinese chive leaf lotus sections are cut into small sections of 0.5-1.0 cm) treated by a specific process and fresh eggs (with shells of 50-60 g) by adopting a hot-pot cold-oil instantaneous frying process (180 + / -10 DEG C fire turning off and cake spreading, and braising in a half-cooked state for 3-5 minutes). In the preparation process, the design of a food-grade stainless steel pot (the inner diameter is larger than or equal to 30 cm) and a detachable anti-sticking layer is adopted, a finished product needs to be subjected to 6kGy irradiation sterilization within 4 hours, and the quality stability is ensured. The invention provides a natural and efficient new choice for treating haemorrhoids, and is especially suitable for stubborn cases with chemical medicine failure.

The present application provides a fermenting product of white lily, which is obtained by fermenting a poria cocos fungus liquid. After the fermenting product of white lily provided by the present application is fermented by the poria cocos fungus, the ester groups in various lycoris tril group in the total lycoris tril are hydrolyzed, so that 4-hydroxy cinnamic acid and other carboxylic acids containing phenolic hydroxyl groups are obtained, which greatly improves the antioxidant property of the fermenting product of white lily. Meanwhile, due to the hydrolysis of lycoris tril, the bitterness of the fermenting product of white lily is reduced, and the taste of the fermenting product of white lily is improved. The present application also provides an extract of the fermenting product of white lily, which is obtained by crushing, sieving and then extracting the fermenting product of white lily with an organic solution.