26 results about "Lycoris" patented technology

The utility model discloses a lycoris breeding device, and relates to the field of breeding devices, the lycoris breeding device comprises a box body, a bearing assembly, a breeding culture dish, a convection fan, a wind shield assembly, a spraying assembly, an LED fluorescent lamp and an electric heating pipe, the left side and the right side of the box body are provided with ventilation windows, and the convection fan is installed on one side of the ventilation windows through a supporting frame; the two wind shield assemblies are arranged on the left side and the right side of the box body respectively, the multiple bearing assemblies are arranged in an inner cavity of the box body in the mode that the positions of the bearing assemblies can be adjusted in the vertical direction, breeding petri dishes are placed in containing grooves formed in the tops of the bearing assemblies, and the multiple spraying assemblies and the LED fluorescent lamps are arranged at the top of the inner cavity of the box body and the bottoms of the bearing assemblies. The electric heating pipe is arranged at the bottom of the cavity of the box. The lycoris breeding device is ingenious in conception and convenient to use, enough living space is provided for breeding and growth of lycoris by adjusting the height distance between the bearing assemblies, meanwhile, the temperature of an inner cavity is rapidly adjusted and controlled, and the uniformity degree of the temperature is effectively improved.

The invention relates to the field of agricultural pest control, and discloses a composite pest control agent based on a plant extract as a core. Comprising 15%-25% of honeysuckle extract, 10%-20% of podophyllum hexandrum extract, 15%-25% of camellia peel extract, 10%-20% of wikstroemia indica extract, 10%-20% of senecio scandens extract, 10%-20% of lycoris radiate extract, 1%-3% of a synergist, 5%-10% of an emulsifier and a solvent. According to the invention, a plurality of plant extracts are fused, and effective prevention and control of a plurality of fungi, bacterial diseases and pests are realized through accurate optimization of the ratio. Compared with a current technology which only adopts a single plant extract, the method has the advantages that the comprehensive prevention and treatment efficiency is remarkably improved, the problems of limited prevention and treatment range and poor multi-pest control effect are successfully solved, and the blank in the research and application field of the compound plant extract prevention and treatment agent is filled.

The invention relates to the technical field of medicines, in particular to a traditional Chinese medicine composition for relieving gout and application thereof. The invention provides a traditional Chinese medicine composition for relieving gout. The traditional Chinese medicine composition comprises the following components in parts by mass: 8-12 parts of an American ginseng extract, 3-6 parts of a poria cocos extract, 6-9 parts of a turmeric extract, 0.8-1.2 parts of a dried ginger extract, 7-13 parts of a cyclocarya paliurus extract, 6-10 parts of a salted cortex phellodendri extract, 4-8 parts of a gynura procumbens extract, 8-12 parts of a nine-processed rhizoma polygonati extract, 8-12 parts of a coix seed extract and 2-6 parts of a corn stigma extract. The traditional Chinese medicine composition can effectively reduce the activity of xanthine oxidase and the content of uric acid in blood, so that gout is relieved, and the effect of the traditional Chinese medicine composition is superior to that of conventionally used western medicine allopurinol.

The invention discloses a method for identifying Phenacoccus solani, and relates to the field of molecular biology. A target for molecular biological identification of Phenacoccus solani, the target is a single sequence target or a target combination consisting of two sequences, the nucleotide sequence of the single sequence target is shown in SEQ ID No.1; the nucleotide sequence of the target combination is shown in SEQ ID No.1 and SEQ ID No.13. The invention provides a method for identifying Phenacoccus solani, which is identified by multi-channel fluorescence quantitative PCR, can improve the accuracy of the detection result, and shorten the detection time.

The invention discloses a method for preparing an in-vitro insect repellent spray for pets by using a lycoris plant extract, which comprises the following steps: S1, raw material pretreatment: cleaning, drying and crushing a lycoris raw material to obtain lycoris powder; s2, extracting the lycoris powder by using an ethanol reflux extraction method to obtain an extraction filtrate; and S3, firstly concentrating the lycoris radiata extract filtrate to obtain a thick lycoris radiata extract concentrate, then performing impurity removal and purification on the concentrate, and after purification is completed, performing concentration again to obtain a pure lycoris radiata extract. The spray has the advantage of being good in parasite expelling effect, and the problems that in the preparation process of an existing pet in-vitro parasite expelling spray, multiple raw materials cannot be evenly mixed conveniently, ingredient distribution is prone to being uneven, and the in-vitro parasite expelling effect on pets is affected are solved.

The invention provides a gender determining gene Rpfmd2 of Eriomycorus lividus and application of the gender determining gene Rpfmd2 in biological prevention and control, and belongs to the technical field of biology. According to the invention, after the expression level of the gene is knocked down in the body of the Adelphocoris indica by using an RNA interference technology, the development of the Adelphocoris indica and the specific differentiation process of the female sex are abnormal, and the female Adelphocoris indica even generates a male specific external genital organ; in addition, the egg laying amount of female insects is reduced, and eggs die. The above abnormal phenomena cause death of the lycoris mellifera, and indicate that the Rpfmd2 gene plays an important role in sex differentiation of the lycoris mellifera. In view of high specificity of the gene participating in regulation and control of insect sex determination, the gene can be used as a target spot for accurately preventing and controlling the apis cerinus, and dsRNA targeting the gene can achieve the purpose of effectively preventing and controlling the apis cerinus by disturbing the sex proportion of the apis cerinus and reducing the population number of the apis cerinus. In conclusion, the invention provides a theoretical basis for biological prevention and control of the apis cerana.

The application provides a LiCOMT gene for regulating synthesis of lycoris kingianus glycosides and application thereof, belongs to the technical field of plant genetic engineering, and the nucleotide sequence of the LiCOMT gene is shown as SEQ ID NO. 7, and the amino acid sequence of the encoded protein is shown as SEQ ID NO. 8; the application screens out a candidate gene LiCOMT significantly related to lycoris kingianus glycoside accumulation from lily bulb development period transcriptome data through weighted gene co-expression network analysis (WGCNA), and confirms that silencing the LiCOMT gene can reduce the content of lycoris kingianus glycoside A, lycoris kingianus glycoside B and lycoris kingianus glycoside F in lily bulbs by about 60%, 42% and 43% respectively by using a virus-induced gene silencing (VIGS) technique; and the application provides important gene resources and theoretical basis for lily secondary metabolism regulation and molecular breeding.

The present invention relates to the field of plant cultivation technology, discloses a method for postponing the flowering of Lycoris bulbs, comprising the following steps: S1, 6-9 months, 1-7 days before the Lycoris bulbs bud, the Lycoris bulbs are dug out and transplanted into a planting box for hypophysing, and a planting matrix is ​​filled in the planting box; S2, the planting box is placed in an indoor environment where a temperature of 34-36 DEG C and a relative air humidity of 80-90% are stored, and the storage is 7-60 days; S3, the temperature is adjusted to 23-25 ​​DEG C and the flowers are destroyed, and the relative air humidity is adjusted to 60-75%; S4, after budding, the flowers are transferred to an outdoor natural environment and grown to flowering. The present invention can postpone flowering for 7-60 days after processing the Lycoris bulbs, so that the flowering process can be delayed according to actual exhibition time arrangements, to meet the postponed viewing needs.

The invention provides application and method of geotrichum galactose yeast NBG001 in improving accumulation of alkaloids for lycoris radiata traditional Chinese medicine, and belongs to the technical field of agricultural production, the geotrichum galactose yeast NBG001 is preserved in China Center for Type Culture Collection on July 7, 2025, and the preservation number is CCTCC NO: M 20251547. By inducing the expression of signal molecules and key genes in the lycoris radiate, the simultaneous accumulation of galanthamine, lycorine and narcissus in the lycoris radiate is remarkably improved.

The invention relates to an oil-control moisturizing composition containing pleione bulbocodioides extract and application of the oil-control moisturizing composition. The composition is prepared from, by mass, 3-8 parts of pleione bulbocodioides extract, 1-5 parts of panax japonicus extract, 1-3 parts of sculellaria barbata extract and 1-2 parts of jasminum grandiflorum extract. The composition prepared by the invention is simple in components, all the components are natural plant sources, the safety is high, all the components have a mutual synergistic effect, and the oil control and moisturizing effects are remarkable.

The present invention provides an extracellular vesicle of a bulbous plant, a preparation method thereof and an application thereof, belonging to the technical field of extracellular vesicle preparation. The preparation method of the extracellular vesicle of the bulbous plant provided by the present invention comprises the following steps: S1, mixing plant tissues with a buffer solution, pulverizing and filtering to obtain a plant stock solution; S2, treating the plant stock solution obtained in step S1 to obtain a plant clear solution; S3, centrifuging the plant clear solution obtained in step S2 at 3-5°C and 110000-130000 g for 80-100 min, retaining the precipitate, resuspending the precipitate with a buffer solution, and repeating 1-2 times to obtain a resuspended solution; S4, filtering the resuspended solution obtained in step S3, and concentrating the filtrate to obtain extracellular vesicles of the plant. This method can efficiently extract extracellular vesicles from viscous fresh lily and lycoris tissues.

The invention provides a preparation method of lycoris plant root tip chromosomes, and belongs to the technical field of biology. The preparation method of the lycoris plant root tip chromosome comprises the steps of root tip induction culture, root tip collection and pretreatment, enzymolysis treatment, chromosome flaking, microscopic observation and the like. A normal-temperature and low-temperature combined 8-hydroxyquinoline double-stage pretreatment mode is adopted for newborn root tips, and a compound enzymatic hydrolysate and a buffer solution pre-preservation system with an optimized ratio are combined, so that the metaphase division cell proportion and the chromosome image quality are effectively improved. The method has the advantages of simplicity and convenience in operation, high efficiency, strong repeatability and the like, is suitable for preparing chromosomes of different types of lycoris radiate and hybrid materials thereof, and can also be popularized and applied to cytogenetics research of other bulb ornamental plants.

The invention provides an ultralow-temperature preservation method of lycoris bulblets, and belongs to the technical field of plant tissue culture. The method comprises the following steps: 1, treating an explant; 2, establishing a tissue culture system; 3, subculturing and inducing the bulblets; 4, pre-culturing the bulblets; 5, cutting bulblets and fixing loading strips; 6, loading and vitrification treatment; 7, freezing, unfreezing and washing; and 8, recovering culture. The rapid tissue propagation and ultralow-temperature preservation method of the lycoris radiate is convenient and efficient, and has reference significance on ultralow-temperature preservation of the lycoris radiate and other bulb and perennial root plant genetic resources.

This invention belongs to the field of plant propagation and cultivation technology, specifically relating to a tissue culture medium and method for the regeneration of Lycoris radiata. This invention provides a tissue culture medium for Lycoris radiata regeneration, comprising a callus induction medium, a callus proliferation medium, and a callus differentiation medium. In the tissue culture medium and method provided by this invention, single scales are selected as explants, and modified MS medium and B5 medium are used as basal media, with appropriate concentrations of 6-BA, NAA, carbon nanotubes, and acid-hydrolyzed casein added. Combined with suitable temperature and light, this facilitates the induction of Lycoris radiata callus proliferation and differentiation, increases the propagation coefficient of Lycoris radiata, and yields a large number of uniform Lycoris radiata tissue culture seedlings.

The invention provides a Chinese ladiestresses root extract as well as a preparation method, a related product and application thereof, and belongs to the technical field of traditional Chinese medicine extraction. The preparation method comprises the following steps: mixing pedicularis chinensis and ketone, and performing reflux leaching and separation to obtain a leaching solution and medicine residues; recovering the solvent from the leaching solution to obtain a Chinese ladiestresses root extract; mixing the medicine residue with water, decocting, centrifuging the decoction, reserving the supernatant, and carrying out alcohol precipitation to obtain crude polysaccharide; reacting the crude polysaccharide with sodium periodate to obtain oxidized paniculate helminthostachys rhizome polysaccharide; and mixing 5-15 parts by weight of the paniculate helichrysum herb extract, 10-30 parts by weight of the oxidized paniculate helichrysum herb polysaccharide and 100 parts by weight of water, and carrying out ultrasonic dispersion and drying to obtain the paniculate helichrysum herb extract. The spiranthes sinensis extract obtained by the method can form a submicron-level particle structure, and can better reduce the foot swelling score of rheumatoid arthritis mice, improve the blood marker index of model mice and reduce the occurrence of hemolysis side effects.

The invention discloses a Penicillium beijeri and Purpureocillium lilacinum complex microbial inoculant and application thereof in preventing and treating green onion and garlic root rot, and relates to the technical field of microorganisms, and the technical scheme is characterized in that the Penicillium beijeri and Purpureocillium lilacinum complex microbial inoculant comprises Purpureocillium lilacinum LS003 and Penicillium beijeri LS004; according to the purpureocillium lilacinus LS003, the Latin name is Paecilomyces lilacinus, and the preservation number of the purpureocillium lilacinus LS003 is CGMCC (China General Microbiological Culture Collection Center) NO.41613; the Latin name of the penicillium bilaiae LS004 is Penicillium bilaiae, and the preservation number of the penicillium bilaiae LS004 is CGMCC (China General Microbiological Culture Collection Center) NO.41614. The disease-preventing and growth-promoting compound microbial agent provided by the invention has good phosphorus-dissolving and growth-promoting functions, and is a good microbial agent which can be used for efficiently promoting growth of lycoris crops.

The invention relates to the technical field of adhesives, and discloses an environment-friendly lycoris starch-vinyl acetate adhesive and a preparation method thereof.The preparation method comprises the steps that water is added into a reaction kettle and stirred, a defoaming agent and polyvinyl alcohol are added, the mixture is heated to 85 DEG C and then self-heated to 90 DEG C, and heat preservation is conducted; cooling to 83 DEG C, adding an emulsifier and part of an initiator, sealing and starting a condenser; slowly dropwise adding vinyl acetate, maintaining the temperature at 77-80 DEG C, and supplementing the initiator every 30 minutes; after dropwise adding, adding the rest initiator, heating to 86 DEG C, and preserving heat; cooling to 83 DEG C, slowly adding lycoris starch paste, and gelatinizing for 30 minutes; and cooling to 65 DEG C, adding the plasticizer dibutyl phthalate, cooling to 50 DEG C, adding the preservative, stirring for 30 minutes, filtering, and packaging to obtain the finished product adhesive. The lycoris starch is introduced into a polyvinyl alcohol-vinyl acetate system, so that the prepared adhesive has the characteristics of high bonding strength, stable viscosity, slight smell and environmental friendliness.

The invention discloses a traditional Chinese medicine composition for treating hypertension, hyperlipidemia and hyperglycemia, and belongs to the technical field of traditional Chinese medicine compositions. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 9 to 15 parts of spica prunellae, 6 to 12 parts of herba artemisiae annuae, 3 to 9 parts of radix notoginseng, 6 to 10 parts of radix rubiae, 0.03 to 0.06 part of cantharides, 3 to 5 parts of myrrh, 3 to 10 parts of radix curcumae, 9 to 15 parts of radix isatidis, 10 to 15 parts of radix salviae miltiorrhizae, 2 to 5 parts of rhizoma coptidis, 3 to 9 parts of rhizoma paridis, 9 to 15 parts of semen plantaginis, 1.5 to 3 parts of lycoris radiate, 4.5 to 9 parts of calyx seu fructus physalis, 3 to 9 parts of cordyceps sinensis, 9 to 15 parts of radix adenophorae, 6 to 12 parts of radix ophiopogonis and 6 to 12 parts of herba dendrobii. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 6-12 parts of radix polygonati officinalis, 6-12 parts of glossy privet fruit, 5-10 parts of liquorice, 15-25 parts of houttuynia cordata, 6-15 parts of alocasia macrorrhiza, 15-30 parts of aleppo avens, 15-30 parts of astragalus membranaceus, 0.1-0.3 part of croton, 1-3 parts of fructus piperis longi, 1-3 parts of cubeb and 5-12 parts of radix achyranthis bidentatae. The invention aims to provide the traditional Chinese medicine composition for treating hypertension, hyperlipidemia and hyperglycemia, which is scientific in compatibility and good in treatment effect. The medicine is used for treating hyperlipidemia.

The invention discloses a long-tube Lycoris radiata LlMYB4 gene and its encoded protein and application, and belongs to the technical field of plant molecular biology. The long-tube Lycoris radiata LlMYB4 gene of the invention has a nucleotide sequence as shown in SEQ ID NO.1 and an amino acid sequence as shown in SEQ ID NO.2. The invention uses the perianth pieces of the long-tube Lycoris radiata in full bloom as materials, obtains the long-tube Lycoris radiata LIMYB4 gene by cloning, constructs its overexpression vector Super1300‑LlMYB4 on this basis, transfers it into Nicotiana benthamiana leaves, obtains transgenic plants, and the results of gene function identification show that the long-tube Lycoris radiata LlMYB4 gene is an important transcription factor that inhibits anthocyanin synthesis or promotes anthocyanin degradation, and has good application prospects in plant growth and development and breeding.

The invention discloses a method for preparing a pet disinfectant by using a lycoris plant extract, which comprises the following steps: S1, lycoris plant extraction: extracting alkaloid in lycoris plants to prepare an alkaloid solution; s2, antibacterial plant extraction: extracting antibacterial disinfection components in antibacterial plants to prepare a supplementary disinfectant; s3, preparing ingredients of the pet disinfectant, namely preparing a surfactant, a humectant, a pH regulator, a buffering agent and an antistatic agent as the ingredients of the pet disinfectant. The method has the advantages of high efficiency and automation, and solves the problems that the production efficiency of the pet disinfectant is low, the extraction effect is unstable, the production cost is high and the like due to the fact that the existing method for preparing the pet disinfectant from the plant extract is inconvenient to efficiently and automatically extract the plant extract in the using process. And the quality of a final product is influenced.

The invention provides traditional Chinese medicine health tea as well as a preparation method and application thereof. The traditional Chinese medicine health tea comprises the following components in parts by weight: 15-20 parts of poria cocos, 15-20 parts of rhodiola rosea, 5-10 parts of gordon euryale seed, 15-20 parts of gynura procumbens, 10-15 parts of Chinese yam, 15-20 parts of salvia miltiorrhiza, 10-15 parts of folium mori, 5-10 parts of dandelion, 5-10 parts of achyranthes bidentata, 5-10 parts of semen cassiae, 2-5 parts of radix pseudostellariae, 5-10 parts of lily, 15-20 parts of bitter gourd, 5-10 parts of sea-buckthorn, 15-20 parts of exocarpium benincasae and 2-5 parts of hairy antler. During preparation, the raw materials are weighed and divided into four groups, the first component and the fourth component are crushed medicine powder, the second component and the third component are water decoction, the water decoction is mixed and concentrated, then is stirred with the first component and then dried in the sun, the dried water decoction and the fourth component are stirred with water, evaporated and fried to be dry, and a tea bag is prepared. The tea has the effects of promoting blood circulation to remove meridian obstruction, improving vigor, preventing cardiovascular and cerebrovascular diseases and the like, and is good in taste, convenient to brew, small in side effect and suitable for long-term drinking. The preparation process ensures the drug effect, can be suitable for large-scale industrial production, and reduces the production cost.

The invention belongs to the technical field of plant preservation, and particularly relates to a compound preservative for lycoris plant fresh cut flowers as well as a preparation method and application of the compound preservative. The compound preservative is prepared from the following effective components in mass concentration: 150 to 200mg / L of citric acid, 15 to 20g / L of cane sugar, 2 to 3g / L of calcium chloride, 1 to 2g / L of chitosan, 1 to 2ppm of nano-silver colloid and 3 to 5mg / L of gibberellin. According to the invention, the nano-silver colloid, the chitosan and the hormone are combined for post-harvest treatment of lycoris plant cut flowers for the first time; four functions of antibiosis, cell energy stabilization, nutrition supply and hormone regulation are integrated, a multi-factor synergistic mechanism is established, and then the postharvest preservation effect of the lycoris plant cut flowers is remarkably improved. Results of the embodiment show that the composite preservative can prolong the life of the lycoris plant cut flower vase to 6-8 days, the phenomena that the base part of the flower stem is curled and the petals are premature senility are obviously reduced, and the good ornamental value is maintained.

The invention relates to a pleione bulbocodioides extract preparation method, a pleione bulbocodioides extract product and application, the preparation method comprises the following steps: crushing pleione bulbocodioides, mixing the crushed pleione bulbocodioides with water, and carrying out ultrahigh pressure treatment to obtain a first mixture; carrying out subcritical extraction on the first mixture, and filtering to obtain an extracting solution; filtering the extracting solution through a nanofiltration membrane to obtain pleione bulbocodioides filtrate; and mixing the pleione bulbocodioides filtrate with a decolorizing agent, filtering, and drying the filtrate to obtain the pleione bulbocodioides decolorizing agent. The method disclosed by the invention is high in extraction efficiency, green and environment-friendly, the obtained extract is rich in active ingredients, can effectively activate the expression of silk polyprotein and paphiopetin, promote the repair of keratinocytes and inhibit sebaceous gland cells from secreting grease, and can maximize the barrier repair and oil control effects of the extract under a specific extraction process.

The use of extracts from plants of the genera *Lycoris* and *Zephyranthes* in the control of rice blast belongs to the field of plant-derived fungicide technology. This paper discloses the uses of extracts from seven *Lycoris* species and one *Zephyranthes* species in the control of rice blast. These extracts can effectively inhibit the mycelial growth and spore germination of *Bacillus oryzae*, and have a good control effect on rice blast. The use of these extracts can delay the development of fungicide resistance in *Bacillus oryzae*.

The invention relates to an allium tuberosum egg composition for treating haemorrhoids as well as a preparation method and application thereof, and belongs to the field of traditional Chinese medicine preparations. According to the composition, a pharmacological structure with a slow-release effect is formed through synergistic compatibility of fresh Chinese chive leaf lotus products (2 cm of rhizomes are removed from 30-50 cm sections, and the Chinese chive leaf lotus sections are cut into small sections of 0.5-1.0 cm) treated by a specific process and fresh eggs (with shells of 50-60 g) by adopting a hot-pot cold-oil instantaneous frying process (180 + / -10 DEG C fire turning off and cake spreading, and braising in a half-cooked state for 3-5 minutes). In the preparation process, the design of a food-grade stainless steel pot (the inner diameter is larger than or equal to 30 cm) and a detachable anti-sticking layer is adopted, a finished product needs to be subjected to 6kGy irradiation sterilization within 4 hours, and the quality stability is ensured. The invention provides a natural and efficient new choice for treating haemorrhoids, and is especially suitable for stubborn cases with chemical medicine failure.

The present application provides a fermenting product of white lily, which is obtained by fermenting a poria cocos fungus liquid. After the fermenting product of white lily provided by the present application is fermented by the poria cocos fungus, the ester groups in various lycoris tril group in the total lycoris tril are hydrolyzed, so that 4-hydroxy cinnamic acid and other carboxylic acids containing phenolic hydroxyl groups are obtained, which greatly improves the antioxidant property of the fermenting product of white lily. Meanwhile, due to the hydrolysis of lycoris tril, the bitterness of the fermenting product of white lily is reduced, and the taste of the fermenting product of white lily is improved. The present application also provides an extract of the fermenting product of white lily, which is obtained by crushing, sieving and then extracting the fermenting product of white lily with an organic solution.