106 results about "Lycoris" patented technology

The invention discloses a normalized planting method of medicinal Lycoris radiate, which comprises the following steps: (1) breeding seed and sprout: reserving seed, (2) planting: selecting ground, grading, planting bed, sowing, breeding, managing sprout, (3) managing field: loosing ground, fertilizing, watering, draining, racking, trimming to remove bud, sealing soil to hibernate, (4) harvesting, grading to satisfy the standard number: GB / T18247.6-2000.

The invention discloses a tissue culture and rapid propagation method for lycoris albiflora. At present, lycoris plants in China mainly perform bulb division propagation and seed self-sowing propagation in the natural state, the propagation coefficient is low, the propagation speed is low, most bulbs can only divide 1-2 child bulbs every year, the number of bulbs is small and limited, and the requirement of commercial production of the lycoris albiflora is far from being met. In order to meet the requirement of commercial production of the lycoris albiflora, in-vitro regenerated plants of the lycoris albiflora are obtained with lycoris albiflora with basal disc bulbs used as explants through processes of adventitious bud induction, subculture, rooting, acclimatization, transplantation and the like, and a tissue culture and rapid propagation technology system for the lycoris albiflora is established to promote the commercial production of the lycoris albiflora.

The invention discloses a method for simultaneously detecting the contents of three alkaloids in a lycoris plant, which is capable of quickly extracting and qualitatively and quantitatively analyzingalkaloid matters of galanthamine, lycoramine and lycorine in the lycoris plant. According to the method disclosed by the invention, gradient eluting is carried out by taking an LC (Liquid Chromatography)-MS (Mass Spectrometry) technology as the basis, analysis is carried out in an MRM (Multiple Reaction Monitoring) mode under positive ion scanning by connecting LC with tandem MS, the three alkaloid matters in the lycoris plant can be quickly extracted and accurately, qualitatively and quantitatively analyzed, the analysis time can be shortened to a large extent, the analysis accuracy is increased, a detection result can be obtained within a program time which is not more than 6.0 minutes, the retention time of the three alkaloids is within 3 minutes, the adding standard recovery rate is 97.2 to 100.2 percent, the RSD (Relative Standard Deviation) is 0.8 to 4.3 percent, and the analysis speed and the analysis accuracy are greatly increased; an effective and accurate analysis method is provided for researching an accumulation law and constructive metabolism regulation of the three alkaloids in the lycoris plant.

Provided are novel use of an extract of Lycoris chejuensis or a compound isolated therefrom for inhibition of β-amyloid production and / or prevention, improvement and / or treatment of a neurodegenerative disease, more specifically a β-amyloid production inhibitor, and / or a composition for prevention or treatment of a neurodegenerative disease containing one or more kinds selected from the group consisting of an extract of Lycoris chejuensis, and / or dihydrolycoricidine, 2-methoxypancracine, lycoricidine, and / or lycoricidinol, as an active ingredient; a method for inhibiting β-amyloid production, and / or preventing and / or treating a neurodegenerative disease using the same; and, a method for preparing the same.

The invention discloses a method for extracting galanthamine from a lycoris plant. The method comprises the following steps: (1) extraction: weighing the treated lycoris powder, adding the lycoris powder into water with pH of 4.5 at a feed-liquid ratio of 1:10 and a unit proportion of g / mL; adding the mixed enzyme of cellulase accounting for 1.0-3.0% of the mass of the lycoris powder and pectinase, wherein the mass ratio of the cellulase to pectinase is 1:1; performing enzymolysis for 20-100min at 50 DEG C; performing ultrasonic treatment for 5-25min and centrifuging for 20min at 5,000r / min; and fetching the supernate to obtain galanthamine extract; and (2) purification: solvent extraction; adsorption by cation exchange resin; C18 reverse phase silica gel column chromatography; normal-phase silica gel column chromatography; recrystallization: dissolving the dried product III obtained in the previous step with ethanol, crystallizing to obtain a crude product of galanthamine, filtering and performing vacuum drying at 50-60 DEG C to obtain galanthamine. The method disclosed by the invention has the advantages of easiness in operation, short extraction time, high extraction rate, small dosage of solvent and relatively low cost; and the purifying process is environment-friendly and the purity is high and can reach 99.2%.

The invention discloses a method for rapid propagation of lycoris plant seedlings through hydroponics and aims at providing a lycoris plant asexual rapid propagation method with high reproductive rate for industrialization production of the lycoris plant seedlings. The technical scheme comprises mechanical cutting and induction of adventitious shoots through water pipelines. The method includes following steps: (1) cutting seedballs on a special lycoris seedball cutting machine according to a shape of a Chinese character mi; (2) soaking mother balls in culture solution for 30 minutes, fishing out and drying in shade; (3) inducing adventitious shoots through the water pipelines; and (4) transplanting in a greenhouse. The method for rapid propagation of lycoris plant seedlings through the hydroponics can greatly improve reproductive rate of lycoris aurea, reproduction ratio of one mother ball is 14 times of that of a natural son ball, and the method is suitable for industrialization large-scale production of the lycoris plant seedlings.

The invention discloses a method for extracting active alkaloid from lycoris plants. The method utilizes the combination of an enzymic method and modern mechanical wall-breaking technique to extract lycoris alkaloid. Lycoris alkaloid is extracted efficiently in excellent yields under mild conditions, other impurities dissolution is lowered and the consistency of extract is decreased for continuing treatment of isolation and purification. Segmental extraction technique is adopted according to the alkalinity of lycoris alkaloid to get a plurality of raw products of lycoris alkaloid. Then highlypurified monomer of lycoris alkaloids (galanthamin, galanthidine, dihydrogalanthamine, sekisamine) are available after column chromatography and repeated recrystallization. The invention has the advantages of simple line, no pollution, short cycle, high yield of products, high safety coefficient, low cost, diversity of outputs, thus raising the comprehensive utilization of lycoris and reducing the manufacturing cost of deep processing and increasing the feasibility of industrial production.