106 results about "Lycoris" patented technology

The invention discloses a normalized planting method of medicinal Lycoris radiate, which comprises the following steps: (1) breeding seed and sprout: reserving seed, (2) planting: selecting ground, grading, planting bed, sowing, breeding, managing sprout, (3) managing field: loosing ground, fertilizing, watering, draining, racking, trimming to remove bud, sealing soil to hibernate, (4) harvesting, grading to satisfy the standard number: GB / T18247.6-2000.

Disclosed is a high-efficiency lycoris and citrus interplanting method. The high-efficiency lycoris and citrus interplanting method includes interplanting lycoris under fruit trees such as citrus, covering the lycoris by rotten animal manure straws, managing by processes of fertilizing, weeding, insecticiding and the like, and harvesting after five-year planting. By the high-efficiency lycoris and citrus interplanting method, two kinds of economic plants can be produced on the same field, multiple cropping index is increased, labor cost is saved, production cost is reduced, yield is increased, and economic benefits are doubly increased.

The invention discloses a method for extracting active alkaloid from lycoris plants. The method utilizes the combination of an enzymic method and modern mechanical wall-breaking technique to extract lycoris alkaloid. Lycoris alkaloid is extracted efficiently in excellent yields under mild conditions, other impurities dissolution is lowered and the consistency of extract is decreased for continuing treatment of isolation and purification. Segmental extraction technique is adopted according to the alkalinity of lycoris alkaloid to get a plurality of raw products of lycoris alkaloid. Then highly purified monomer of lycoris alkaloids (galanthamin, galanthidine, dihydrogalanthamine, sekisamine) are available after column chromatography and repeated recrystallization. The invention has the advantages of simple line, no pollution, short cycle, high yield of products, high safety coefficient, low cost, diversity of outputs, thus raising the comprehensive utilization of lycoris and reducing the manufacturing cost of deep processing and increasing the feasibility of industrial production.

The invention discloses a technology method for distilling short-tube lycoris alkali from short-tube lycoris plants. It supplies the technology process and condition to distill short-tube lycoris alkali from the stem tuber of short-tube lycoris through cellulose enzyme enzymolysis. The purity of the product could reach 99%. And the method is easy to operate, and high efficient. The yield could reach 4.69 per ten thousand.

The invention discloses a Chinese herbal medicine control agent for common diseases of strawberries and a preparation method thereof, belonging to the technical field of Chinese herbal medicine pesticides. The Chinese herbal medicine control agent is prepared from the following raw materials: 3-13 parts of lily, 2-8 parts of malt, 3-10 parts of lotus leaf, 3-12 parts of areca seed, 2-6 parts of peppermint leaf, 3-10 parts of villous amomum fruit, 3-12 parts of lychee seed, 2-13 parts of golden larch, 3-11 parts of shorttube lycoris, 1-8 parts of dwarf lilyturf tuber, 3-12 parts of hawthorn leaf, 3-11 parts of garlic, 2-8 parts of gambir plant, 2-7 parts of officinal magnolia bark, 1-7 parts of himalayan teasel root, 2-9 parts of rabbit dung, 1-6 parts of pyrrosia leaf, 2-9 parts of safflower, 3-10 parts of combined spicebush root, 3-10 parts of frankincense, 3-12 parts of myrrh and 3-13 parts of common nandina fruit. The preparation method comprises the steps of raw material treatment, extraction, spray drying, drying and packaging. The Chinese herbal medicine control agent for common diseases of strawberries has the beneficial effects that the raw materials are natural materials and the components are nontoxic, residue-free and pollution-free; the production method of the Chinese herbal medicine control agent is simple, is easy to operate, is low in production cost and is convenient to use.

The invention discloses a microsatellite deoxyribonucleic acid (DNA) molecular marker of lycoris. 33 microsatellite DNA-containing clones are obtained and 10 microsatellite molecular markers with rich polymorphism, namely Lyra-1, Lyra-2, Lyra-3, Lyra-4, Lyra-5, Lyra-6, Lyra-7, Lyra-8, Lyra-9 and Lyra-10, are determined by establishing a lycoris microsatellite (CT)n enrichment library, screening and sequencing the positive clones of the microsatellite. The marker can be used for researching genetic diversity protection of the lycoris and variation and evolution relation between species and populations of lycoris plants and identifying the varieties of the lycoris; and the marker has high repeatability and is a reliable and effective molecular marker.

The invention discloses a process of extracting dihydrogalanthamine from Lycoris radiata plant, which in detail relates to a method for separating dihydrogalanthamine exquisite from alkaloid extracted from Lycoris radiata plant, and it is a recombined method of enzyme technology and chromatography. It comprises process and condition of extraction and separation of coarse lycorine, then extracting filtering liquid of coarse lycorine, condensing, preparing column material, loading column, eluting, condensing, recrystallizing and getting dihydrogalanthamine with purity of over 99%. The invention is different from method using organic solvent for dihydrogalanthamine extraction, and it can obtain coarse lycorine, coarse galantamin and dihydrogalanthamine with purity over 99%.

The invention provides a method for inducing adventitious buds by adopting Lycoris radiate rachis as explant in order to obtain regenerated plants. The method comprises the following steps: selection and disinfection of the explant, induction of the adventitious buds, subculture and propagation, root induction, and transplantation of tissue culture plants. The method allows the induction germination rate to reach 96%, 4.9 times or above adventitious buds to be proliferated from a per plant and the rooting rate to reach 94%, and can effectively solve the problem of in vitro rapid propagation of Lycoris radiate.

The invention relates to a method for biologically preventing and controlling crop diseases and insect pests, in particular to a method for interplanting lycoris and pyrethrum to prevent and control crop disease and insect pests. The method includes planting the lycoris and the pyrethrum on the peripheries of crop cultivation lands in a matched manner to achieve purposes of preventing and controlling the crop diseases and insect pests. The method has the advantages that ecological environments of the cultivation lands can be improved, the crop diseases and insect pests can be controlled for the cultivation lands without chemical pesticides, accordingly, the amounts of pesticides applied to main crops of the cultivation lands can be greatly reduced, pesticide residues of the crops can be guaranteed against exceeding standards, and the organic crops can be manufactured; the lands can be sufficiently utilized, so that the environment can be beautified.

A preparation method of a traditional Chinese medicine lotion for treating redness-swelling type cellulitis belongs to the technical field of traditional Chinese medicine preparation methods. Currently, antibiotics and sulfanilamide medicaments are generally used for the treatment of redness-swelling type cellulitis, and have the disadvantage of great side effect. The technical scheme of the invention is that the preparation method comprises the following steps: soaking the following 28 herbs of polygonum hydropiper, common lantana leaf, sedum, shrubby breynia, phymatopsis hastata, herba patriniae, changium smyrnioide, hemsleya macrosperma, rhizoma anemarrhenae, radix tinosporae, nasturtium, honeysuckle, bidens biternata, houttuynia, herba lespedezae cuneatae, hypericum perforatum, sedum sarmentosum bunge, sagina subulata, lycoris, patience dock root, radix sophorae flavescentis, balsam pear, folium llicis latifoliae, elephantopus scaber, gentrin knotweed, saxifrage, solanum nigrum and licorice in water, decocting with mild fire, filtering to remove residues so as to obtain the medicinal liquid which is the traditional Chinese medicine lotion for treating redness-swelling type cellulitis. The advantages are that the prepared traditional Chinese medicine is less in toxic and side effect, short in treatment course, and high in cure rate, and can also prevent adverse effect caused by western medicine administration.

The invention discloses a tissue culture and rapid propagation method for lycoris albiflora. At present, lycoris plants in China mainly perform bulb division propagation and seed self-sowing propagation in the natural state, the propagation coefficient is low, the propagation speed is low, most bulbs can only divide 1-2 child bulbs every year, the number of bulbs is small and limited, and the requirement of commercial production of the lycoris albiflora is far from being met. In order to meet the requirement of commercial production of the lycoris albiflora, in-vitro regenerated plants of the lycoris albiflora are obtained with lycoris albiflora with basal disc bulbs used as explants through processes of adventitious bud induction, subculture, rooting, acclimatization, transplantation and the like, and a tissue culture and rapid propagation technology system for the lycoris albiflora is established to promote the commercial production of the lycoris albiflora.

The invention discloses a method for measuring pollen vitality of lycoris plants using an in-vitro germination method, comprising the following steps: collecting pollens and placing the pollens into culture solution to perform in-vitro culture, wherein formula of the culture solution consists of 30-80 g / L of sucrose and 5-25 mg / L of boric acid; the temperature for the in-vitro culture is 25-35 degrees centigrade; the time for the in-vitro culture is 60-90 min; and the relative humidity for the in-vitro culture is 70-90%. According to the invention, in-vitro culture conditions of the pollens of lycoris plants are explored; a simple, rapid and feasible method for measuring the pollen vitality of the lycoris plants is provided; and basis for cross breeding of the lycoris plants is provided.

The invention relates to a method for using lycoris herb to produce fuel ethanol, which takes solid bulb of the lycoris herb as raw material to obtain the fuel ethanol and simultaneously to produce by-product of lycoramine. The method of the invention researches the process steps and the process conditions for utilizing the lycoris herb to produce the fuel ethanol through experiments according to the content characteristics of chemical constituents of the solid bulb of the lycoris herb, simultaneously, a plurality of alkaloid with comparatively high lycoris medicinal value is separated, the method of invention is a compound process technique for preparing a plurality of products, more than 95% starch in lycoris can be transformed into the ethanol, the process steps are simple, the using time is little, the operation is convenient, pollution is not produced, and the method is suitable for utilizing the lycoris to produce the fuel ethanol with large scale industrialization.

The invention provides a cultivation method of lycoris plants. The method comprises the following steps that LED light quality is used for treating lycoris plant seedlings, wherein the LED light quality is blue light (B) or red and blue compound light (RB). The content of alkaloid type ingredients of galanthamine, dihydrogalanthamine and lycorine in the lycoris plants cultivated by the method is obviously improved; the operation of the method is simple and convenient; the production period is short; the method is applicable to factory production; important significance is realized for meetingthe industrialized production of lycoris anticancer alkaloid.

The invention relates to a lycoris spp. plant fluorescence EST-SSR molecular marker and application thereof, and belongs to the technical field of molecular markers. The molecular marker comprises QZ209, QZ155, QZ175 and QZ171. The molecular markers QZ209, QZ155, QZ175 and QZ171 can completely distinguish 16 pieces of lycoris spp. species, wherein a number of polymorphism sites of a primer of theQZ209 is 12, a PIC (polymorphism information content) value is 0.92, and different species of materials of 12 pieces of lycoris spp. can be distinguished through independent application of the QZ209,the QZ209 can be used as an excellent SSR molecular marker to be applied to subsequent classification and identification work of the lycoris spp. species, and a scientific basis is provided for the protection and development of lycoris spp. resources.

The invention relates to an in-vitro probulb division growth method for realizing rapid proliferation of Lycoris herbs, which belongs to the technical field of rapid proliferation of plants. The method comprises the following steps: germination culture of seeds; proliferation culture of probulbs; and swelling culture of adventitious buds until substantially-swollen small bulbs are obtained. According to the invention, the probulbs obtained after seed germination are used as explants, and proliferation culture and swelling culture are carried out through basal disc cutting of in-vitro bulbs; so the rate of pollution is substantially reduced; loss of wild seedball resources of the Lycoris herbs is decreased; and a great number germplasm populations with a high growth synchronization degree and consistent properties can be efficiently obtained in a short period.

The invention relates to a trans-cinnamic acid-4-hydroxylase and a coding gene and application thereof. The trans-cinnamic acid-4-hydroxylase of lycoris aurea is disclosed for the first time; and the trans-cinnamic acid-4-hydroxylase has good substrate specificity and regioselectivity, and hydroxylation of 4-bit C of trans-cinnamic acid can be catalyzed to generate p-cumaric acid. The invention further discloses a polynucleotide of coding the trans-cinnamic acid-4-hydroxylase, a carrier and host cell for expressing the trans-cinnamic acid-4-hydroxylase, and a method for producing the p-cumaric acid.

The invention relates to a cytochrome P450 reductase and a coding gene and application thereof. The invention discloses a cytochrome P450 reductase 2 of an amaryllidaceae plant lycoris aurea for the first time, and the cytochrome P450 reductase 2 has good coenzyme specificity and can assist a cytochrome P450 enzyme to develop the catalytic activity to oxidatively modify a substrate synthetic product thereof. The invention further discloses a polynucleotide for coding the cytochrome P450 reductase, a carrier for expressing the cytochrome P450 reductase and a method for producing a caffeic acid.

The invention relates to cytochrome P450 reductase as well as coding gene and application thereof. The invention discloses cytochrome P450 reductase 1 of lycoris aurea as an amaryllidaceae plant for the first time; the cytochrome P450 reductase 1 has favorable coenzyme specificity and can be used for assisting a cytochrome P450 enzyme in exerting catalytic activity to modify a synthetic product of a substrate of the cytochrome P450 enzyme in an oxidative manner. The invention also discloses a polynucleotide for coding the cytochrome P450 reductase, a carrier and a host cell for expressing the cytochrome P450 reductase and a method for producing p-coumaric acid.

The invention relates to p-coumaric acid-3-hydroxylase as well as a coding gene and an application thereof. The p-coumaric acid-3-hydroxylase of lycoris plant lycoris aurea is disclosed for the first time, has good substrate specificity and region selectivity, and can catalyze hydroxylation of 3-position C of p-coumaric acid to produce coffeic acid. The invention further discloses polunecleotide coding the p-coumaric acid-3-hydroxylase, a vector and a host cell expressing the p-coumaric acid-3-hydroxylase as well as a coffeic acid production method.

The invention provides a traditional Chinese medicine for treating arrhythmia due to heart-spleen deficiency and a preparation method thereof. The traditional Chinese medicine for treating the arrhythmia due to heart-spleen deficiency is prepared from the following raw materials: stropanthus divericatus, lycoris, coriaceous knotweed rhizome, celery, wilson begonia rhizome, fragrant sarcococca herb, sambucus adnata wall, mimosa pudica, corallodiscus flabellate, cinnabar, hairyvein agrimony, grass, orchidaceae ludisia, dioscorea pentaphylla, companumoea root, panicled fameflower root, dillseed, madder root and humulus lupulus. The traditional Chinese medicine for treating the arrhythmia due to heart-spleen deficiency has the beneficial effects that the theory of traditional Chinese medicines is utilized, and the advantages of quick effect, definite therapeutic effect, high safety, no toxic or side effect and difficulty in recurrence can be realized when the traditional Chinese medicine provided by the invention is used for treating the arrhythmia due to heart-spleen deficiency.

The invention discloses a method for simultaneously detecting the contents of three alkaloids in a lycoris plant, which is capable of quickly extracting and qualitatively and quantitatively analyzingalkaloid matters of galanthamine, lycoramine and lycorine in the lycoris plant. According to the method disclosed by the invention, gradient eluting is carried out by taking an LC (Liquid Chromatography)-MS (Mass Spectrometry) technology as the basis, analysis is carried out in an MRM (Multiple Reaction Monitoring) mode under positive ion scanning by connecting LC with tandem MS, the three alkaloid matters in the lycoris plant can be quickly extracted and accurately, qualitatively and quantitatively analyzed, the analysis time can be shortened to a large extent, the analysis accuracy is increased, a detection result can be obtained within a program time which is not more than 6.0 minutes, the retention time of the three alkaloids is within 3 minutes, the adding standard recovery rate is 97.2 to 100.2 percent, the RSD (Relative Standard Deviation) is 0.8 to 4.3 percent, and the analysis speed and the analysis accuracy are greatly increased; an effective and accurate analysis method is provided for researching an accumulation law and constructive metabolism regulation of the three alkaloids in the lycoris plant.

The invention provides a lycoris radiata colloid extracted from lycoris radiate plant bulb and an extraction method thereof. The lycoris radiata colloid is obtained by processing and extracting the raw material of lycoris radiate plant bulb. The method comprises the following process steps: (1) preparing raw material; (2) preparing serosity; (3) centrifuging the slag and obtaining clean liquid; (4) preparing decoloring liquid; (5) preparing a crude product of lycoris radiata colloid; (6) preparing a semi-finished product of lycoris radiata colloid; and (7) preparing finished product lycoris radiata colloid. The lycoris radiata colloid can be a product used for replacing Arabic gum. The method can extract the lycoris radiata colloid and separate the lycoris radiata total alkaloids, the process is simple, and the energy resource consumption is saved.

Provided are novel use of an extract of Lycoris chejuensis or a compound isolated therefrom for inhibition of β-amyloid production and / or prevention, improvement and / or treatment of a neurodegenerative disease, more specifically a β-amyloid production inhibitor, and / or a composition for prevention or treatment of a neurodegenerative disease containing one or more kinds selected from the group consisting of an extract of Lycoris chejuensis, and / or dihydrolycoricidine, 2-methoxypancracine, lycoricidine, and / or lycoricidinol, as an active ingredient; a method for inhibiting β-amyloid production, and / or preventing and / or treating a neurodegenerative disease using the same; and, a method for preparing the same.

The invention discloses a method for extracting galanthamine from a lycoris plant. The method comprises the following steps: (1) extraction: weighing the treated lycoris powder, adding the lycoris powder into water with pH of 4.5 at a feed-liquid ratio of 1:10 and a unit proportion of g / mL; adding the mixed enzyme of cellulase accounting for 1.0-3.0% of the mass of the lycoris powder and pectinase, wherein the mass ratio of the cellulase to pectinase is 1:1; performing enzymolysis for 20-100min at 50 DEG C; performing ultrasonic treatment for 5-25min and centrifuging for 20min at 5,000r / min; and fetching the supernate to obtain galanthamine extract; and (2) purification: solvent extraction; adsorption by cation exchange resin; C18 reverse phase silica gel column chromatography; normal-phase silica gel column chromatography; recrystallization: dissolving the dried product III obtained in the previous step with ethanol, crystallizing to obtain a crude product of galanthamine, filtering and performing vacuum drying at 50-60 DEG C to obtain galanthamine. The method disclosed by the invention has the advantages of easiness in operation, short extraction time, high extraction rate, small dosage of solvent and relatively low cost; and the purifying process is environment-friendly and the purity is high and can reach 99.2%.

The invention relates to lycoris plant lycoris aurea 1-aminocyclopropane-1-carboxylic acid synthetase as well as a coding gene and application thereof. The lycoris plant lycoris aurea 1-aminocyclopropane-1-carboxylic acid synthetase is disclosed for the first time, and has good enzymatic activity and can catalyze adenosyl methionine to synthesize the 1-aminocyclopropane-1-carboxylic acid. The invention also discloses a polynucleotide for coding the 1-aminocyclopropane-1-carboxylic acid synthetase, a vector and a host cell for expressing the 1-aminocyclopropane-1-carboxylic acid synthetase, and a method for producing the 1-aminocyclopropane-1-carboxylic acid by using a whole-cell transformation method.

Provided are a pest control waterlogged compost fertilizer and a preparation method thereof. Chinese wisteria powder, oleander powder, lycoris radiate powder, delphinium ajacis powder, hyacinth powder, crinum asiaticum powder, hedera helix powder, monstera deliciosa powder, ricinus communis powder, zephyranthes candida powder and the like are added into waterlogged compost, so that the pest control waterlogged compost fertilizer prepared according to the proportion not only can prevent the waterlogged compost from rotting and stinking, but also can be used as a fertilizer for vegetables such as radishes and potatoes, and plants such as fruit trees and flowers, and can prevent the breeding of pests in the soil. The insect killing effect is good when the pest control waterlogged compost fertilizer is used, the growth condition of plants using the pest control waterlogged compost fertilizer is also very good, and the growth of the plants can be promoted; vegetables such as radishes and potatoes, and plants such as fruit trees and flowers can be irrigated with the pest control waterlogged compost fertilizer; and the pest control waterlogged compost fertilizer can kill pests in soil, also can promote plant growth, is pollution-free and non-toxic, and plays an important role in protecting the environment.

The invention relates to a method for extracting lycorine from Lycoris plants, which comprises the following steps: 1. extraction: weighing Lycoris plant powder which is subjected to drying, pulverization and screening, adding water with the pH value of 1.5-7.5 according to the material-liquid ratio (g / mL) of 1:(5-25), adding an enzyme which accounts for 1-5 wt% of the Lycoris plant powder, carrying out enzymolysis at 40-80 DEG C for 0.5-2.5 hours, centrifuging for 20 minutes, filtering, and taking the supernate to obtain a lycorine extracting solution; 2. purification: (1) carrying out solvent extraction; (2) adsorbing with a cation exchange resin; (3) carrying out C18 reversed-phase silica gel column chromatography; (4) carrying out normal-phase silica gel column chromatography; and (5) recrystallization: dissolving the dried substance III obtained in the step (4) in ethanol, recrystallizing to obtain a lycorine crude product, filtering, and carrying out vacuum drying at 50-60 DEG C to obtain the lycorine. The method is simple to operate, and has the advantages of low production cost, no environment pollution, short reaction time and higher extraction yield (30.46% higher than other methods). The purification technique has the advantages of environment friendliness and high purity (up to 99.3%).

The invention discloses a method for rapid propagation of lycoris plant seedlings through hydroponics and aims at providing a lycoris plant asexual rapid propagation method with high reproductive rate for industrialization production of the lycoris plant seedlings. The technical scheme comprises mechanical cutting and induction of adventitious shoots through water pipelines. The method includes following steps: (1) cutting seedballs on a special lycoris seedball cutting machine according to a shape of a Chinese character mi; (2) soaking mother balls in culture solution for 30 minutes, fishing out and drying in shade; (3) inducing adventitious shoots through the water pipelines; and (4) transplanting in a greenhouse. The method for rapid propagation of lycoris plant seedlings through the hydroponics can greatly improve reproductive rate of lycoris aurea, reproduction ratio of one mother ball is 14 times of that of a natural son ball, and the method is suitable for industrialization large-scale production of the lycoris plant seedlings.

The invention discloses a method for extracting active alkaloid from lycoris plants. The method utilizes the combination of an enzymic method and modern mechanical wall-breaking technique to extract lycoris alkaloid. Lycoris alkaloid is extracted efficiently in excellent yields under mild conditions, other impurities dissolution is lowered and the consistency of extract is decreased for continuing treatment of isolation and purification. Segmental extraction technique is adopted according to the alkalinity of lycoris alkaloid to get a plurality of raw products of lycoris alkaloid. Then highlypurified monomer of lycoris alkaloids (galanthamin, galanthidine, dihydrogalanthamine, sekisamine) are available after column chromatography and repeated recrystallization. The invention has the advantages of simple line, no pollution, short cycle, high yield of products, high safety coefficient, low cost, diversity of outputs, thus raising the comprehensive utilization of lycoris and reducing the manufacturing cost of deep processing and increasing the feasibility of industrial production.