68 results about "Hirudin" patented technology

The invention provides an extraction method of hirudin, an oral preparation containing the hirudin and a preparation method of the oral preparation, and belongs to the technical field of leech processing and traditional Chinese medicine. The hirudin extraction method comprises the following steps: S1, soaking fresh leeches in a freeze-drying protective agent, taking out the leeches, wiping the surfaces of the leeches, pre-freezing the leeches at the temperature of 40 DEG C below zero to 50 DEG C below zero for 1-2 hours, and freeze-drying the leeches at the temperature of 20 DEG C below zero to 30 DEG C below zero for 4-6 hours to obtain freeze-dried leeches; s2, crushing the freeze-dried leeches, adding the crushed leeches into water, adding enzyme and lecithin, performing enzymolysis for 4-6 hours under the conditions that the temperature is 35-40 DEG C and the pH value is 7.5-8.5, and cooling to room temperature to obtain an enzymolysis product; s3, sterilization, ultrafiltration and drying are conducted, and hirudin is obtained. The hirudin extracted by the method is high in activity and high in recovery rate.

The invention discloses a natural hirudin extraction method based on cell fermentation. The natural hirudin extraction method comprises the following steps: S1, providing leeches, and carrying out starvation treatment on the leeches; s2, taking out the leeches, cleaning the leeches with a metaldehyde solution, and then putting the leeches into purified water for cleaning; s3, inducing and stimulating the leeches, and collecting secretory fluid; s4, extracting hirudin in the secretion on the basis of cell fermentation to obtain fermentation liquor; s5, carrying out separation and purification treatment on the fermentation liquor based on ammonium sulfate fractional precipitation salting-out; and S6, further separating and purifying the fermentation liquor based on ion exchange chromatography. According to the method, the components of the leeches are utilized, the extraction rate of the hirudin is further improved through cell fermentation, a two-stage separation and purification system is established, the purification process is optimized, and the recovery rate and the purification effect of the hirudin are further improved.

The invention relates to the technical field of probiotic beverages, in particular to a hirudin peptide probiotic beverage with a vascular scavenger function, which is prepared from the following components in percentage by mass: 1 to 10 percent of hirudin peptide, 0.1 to 5 percent of probiotics, 2 to 5 percent of prebiotics, 1 to 5 percent of fruit juice, 0.1 to 2 percent of vitamin, 0.1 to 1 percent of mineral substance, 0.5 to 2 percent of dietary fiber, 0.1 to 1 percent of natural extract, 0.1 to 1 percent of sweetening agent, 0.01 to 0.1 percent of preservative and 70 to 90 percent of water, the hirudin peptide in the beverage serves as a core component of the beverage, has remarkable anticoagulation and thrombolysis effects and can activate a fibrin dissolution system in blood and inhibit the activity of thrombin, so that thrombosis is effectively prevented and treated, the occurrence risk of cardiovascular and cerebrovascular events is reduced, and the hirudin peptide can also expand blood vessels, increase blood flow and promote blood circulation. Microcirculation is improved, the symptoms of palpitation, chest distress, paralysis of hands and feet, dizziness and headache can be relieved, and blood circulation and thrombosis resistance are improved.

The invention discloses a fusion protein rHTA and application thereof, the fusion protein rHTA is sequentially composed of a Hirudin protein, a thrombin cleavage site and an AnxV protein from the N terminal to the C terminal, and the amino acid sequence is as shown in SEQ ID NO: 4 from the 1st site to the 393th site from the N terminal. Experiments prove that the fusion protein rHTA is specifically activated by thrombin at the local part of thrombus and releases AnxV protein and Hirudin protein, and the AnxV protein can specifically recognize and combine with PS, so that the fusion protein rHTA is enriched at the thrombosis part containing platelets in a targeted manner, the Hirudin protein is locally released under the action of thrombin, local precise anticoagulation is realized, and the anticoagulation efficiency is further enhanced. Therefore, the fusion protein rHTA provided by the invention not only has high anticoagulant activity, but also realizes enrichment and release at the thrombus formation part, and reduces the bleeding risk. The method has an important application value.

This invention relates to the field of health products or food technology, and more specifically to a composition and its application in removing dampness and controlling blood sugar, balancing blood pressure, and regulating blood lipids. The invention provides a composition for removing dampness and controlling blood sugar, balancing blood pressure, and regulating blood lipids, comprising Poria cocos extract, Astragalus membranaceus extract, hirudin, and at least one or more of Eucommia ulmoides extract, Gynostemma pentaphyllum extract, and Morus alba twig extract. The composition provided by this invention utilizes extracts from at least four precious Chinese medicinal herbs to exert a synergistic effect, jointly achieving the effects of lowering blood pressure, removing dampness and lowering blood sugar, and lowering blood lipids.

The invention discloses a hirudin escherichia coli engineering bacterium with controllable production generations as well as a construction method and application thereof, and relates to the technical field of biological medicines. The invention discloses a construction method of hirudin escherichia coli engineering bacteria with controllable production generations, which comprises the following steps: constructing a hokC protein gene as shown in the sequence of SEQ ID NO.1 and a constitutive promoter trxA as shown in the sequence of SEQ ID NO.2 into a recombinant expression vector pET28a (+)-ptrxA-hokC, and then transforming the recombinant expression vector into a hirudin synthetic strain E. coli Hirudin HV1-3 to obtain the hirudin escherichia coli engineering bacteria with controllable production generations. Through multiple rounds of culture, the hirudin yield of the engineering strain is reduced to 0 in the tenth round. The method is suitable for various hirudin types, and has the characteristics of reliable operation and high safety.

The invention discloses a recombinant leech polypeptide with high anticoagulant activity as well as a preparation method and application thereof. A PelB-T2Y-N47K-N52Y mutant is constructed by carrying out structural optimization on a hirudin HV2 sequence, a polypeptide product with relatively high anticoagulant activity is obtained after induced expression and nickel affinity chromatography purification are carried out in a prokaryotic expression system, and the anticoagulant activity of the polypeptide product reaches 9135.8 ATU / mg and is remarkably superior to that of an initial sequence HV2. Meanwhile, the polypeptide shows a lower IC50 value in a hydroxyl radical scavenging experiment, and has the activity of inhibiting generation of AGEs in a non-enzymatic saccharification model. Molecular docking and binding free energy analysis show that the combination of the mutant and thrombin (PDB ID: 4HTC) is more stable. The leech polypeptide disclosed by the invention has the potential of being developed into a multifunctional bioactive peptide preparation, and is suitable for the related fields of anticoagulation, antioxidation and anti-saccharification.

The invention relates to an exosome with high permeability and application of the exosome, and the surface of the exosome is connected with dioleoyl phosphatidylserine, 1, 2-dioleoyl-sn-glycerol-3-phosphorylethanolamine and hirudin. The exosome constructed by the invention can improve the permeability of the skin, can carry active components to directly reach the skin epidermis and corium layer, is accumulated on the epidermis and corium layer, continuously provides nutrition for the skin, and fully exerts the skin care and beautifying effects.

The invention discloses a leech extract and a preparation method and application thereof, and relates to the technical field of leech extract preparation, the leech extract comprises leech oil, hirudin, mixed polypeptide and a small molecule extract, the small molecule extract is a small molecule compound and trace elements, the small molecule compounds are small molecule oligopeptides, pyridines, glycolipids, carboxylic esters and steroids, and the trace elements comprise Fe, Mn, Zn and Co; according to the leech extract and the preparation method and application thereof, through low-temperature, high-pressure and organic solvent subcritical efficient extraction, living leeches are instantly killed, biological enzymes are inactivated, leech oil in the leeches is effectively extracted, peculiar smells are removed, meanwhile, thermosensitive functional components are protected, and the extraction efficiency of hirudin is improved; ultrasonic extraction is performed through a continuous countercurrent ultrasonic extractor, so that the leaching efficiency of the hirudin is improved; neutral or alkaline protease is used for enzymolysis, protein is converted into high-activity oligopeptide, the utilization rate of effective components is increased, and the peculiar smell of the product is reduced.

The invention provides a hirudin separation and purification method and system. The method comprises the following steps: grinding leeches into slurry; adding trypsin into the serous fluid, and then carrying out hydrolysis; performing counter-current extraction on the enzymatic hydrolysate obtained after hydrolysis; carrying out centrifugal separation on extract obtained by counter-current extraction, and collecting supernate; performing ultrafiltration concentration on the supernate by using an ultrafiltration membrane to obtain a concentrated solution; adsorbing hirudin in the concentrated solution by using DEAE-Sepharose FF resin, eluting, and then collecting an eluent; and carrying out vacuum freeze drying on the eluent to obtain freeze-dried powder. The system comprises a purification tank, a grinding device, an enzymolysis tank, a counter-current extraction device, a centrifugal machine, an ultrafiltration concentrator, an ion exchange chromatography instrument and a freeze dryer. In the hirudin production process, conditions are mild, damage to heat-sensitive components is small, high-toxicity organic solvents are not used, green and environment-friendly effects are achieved, and the prepared hirudin is high in activity retention rate and purity.

The present invention provides a freckle-removing and whitening essence and a preparation method thereof, belonging to the technical field of cosmetics. It is prepared from the following raw materials in parts by weight: 10-20 parts of plant extract, 3-5 parts of hirudin / phloretin / ellagic acid chelate complex, 10-20 parts of emollient, 5-10 parts of humectant, 0.3-1 part of thickener, 0.5-3 parts of emulsifier, and 40-60 parts of deionized water. The freckle-removing and whitening essence prepared by the present invention has good effects of whitening, freckle-removing, firming the skin, antioxidant, anti-inflammatory, moisturizing, improving skin elasticity and delaying aging, and has wide sources of raw materials and simple preparation method, and has broad application prospects.

The invention provides an active quick-acting thrombolytic drug preparation device and a preparation method thereof. The invention provides an active quick-acting thrombolytic medicine which is prepared from the following raw material medicines in parts by weight: 3-9 parts of ligusticum wallichii extract, 6-18 parts of panax notoginseng saponins, 2-5 parts of panax notoginseng powder, 2-4 parts of active hirudin, 8-24 parts of fresh and alive earthworms and 0.5-1.5 parts of borneol, and biological induction liquid is prepared from the fresh and alive earthworms, panax notoginseng powder and purified water. The leeches are put into the cotton layer, so that the leeches suck little induction liquid and secrete a large amount of saliva, and active hirudin serous fluid can be conveniently extracted; the active quick-acting thrombolytic drug preparation device and the preparation method thereof have the advantages that non-drug composition induction liquid is not used, emetic is not needed, hurt to living leeches is avoided, the survival rate of the leeches is not affected, hirudin extraction does not need high-temperature drying, the activity of hirudin is kept, and the curative effect of the drug is guaranteed.

The device comprises a feeding assembly, a liquid storage box and a chromatography assembly, the liquid storage box and the chromatography assembly are connected with the feeding assembly, the feeding assembly is provided with an outer cylinder, a rotating cylinder and a sealing cavity from outside to inside, and the rotating cylinder is driven by a driving assembly to rotate and then communicates with the liquid storage box and the feeding assembly; or the chromatography assembly and the feeding assembly are communicated, and finally the elution agent is added into the chromatography assembly through the piston. The elution agent can be directly injected into the chromatography assembly in a closed environment, exposure in a laboratory is avoided, quantitative adding can be achieved, and the volume of the elution agent in the chromatography assembly is strictly controlled.

The invention belongs to the technical field of nano preparations, and particularly relates to a hirudin nano drug for relieving chronic asthma and a preparation method thereof.The hirudin nano drug is prepared from, by weight, 0.5-1 part of hirudin prodrug, 10-15 parts of stabilizer, 40-80 parts of freeze-drying protective additive and 1.5 parts of histidine; the hirudin prodrug molecule is composed of hirudin, MMP-9 responsive peptide, ROS responsive thioether bond and hydrophobic hexadecanoic acid, can spontaneously assemble to form a nano-sized prodrug under hydrophilic and hydrophobic effects, has stability in systemic circulation and is targeted and enriched at an asthma airway inflammation part; the hirudin with the thrombin activity inhibition effect is responsively released at the focus, so that the effect and the purpose of slowly treating the asthma are achieved.

Provided are a method for extracting a hirudin mutant, HV2-Lys47, from a fermentation broth of the hirudin mutant HV2-Lys47 produced by the fermentation of Escherichia coli, and purifying same, the use of the hirudin mutant HV2-Lys47 in an anticoagulated blood collection tube, and an anticoagulated blood collection tube containing the hirudin mutant HV2-Lys47. In the purification method, a membrane technology and a primary column chromatography technology are used to obtain the hirudin mutant HV2-Lys47.

The present application belongs to the technical field of biological medicine separation and purification, and particularly relates to a leech-derived hirudin separation and purification method based on thrombin affinity magnetic microspheres and application thereof. The present application uses self-prepared leaching liquor to coarsely extract hirudin from leech powder, then selects carboxyl magnetic microspheres to prepare thrombin affinity magnetic microspheres, and further separates and purifies by using the thrombin affinity magnetic microspheres, so as to finally obtain natural hirudin with an activity of 17000 ATU / g or more. The self-prepared leaching liquor can destroy the cell wall of leech, so that the hirudin in the leech is fully dissolved, thereby providing higher efficiency for the coarse extraction of hirudin in leech powder. The prepared thrombin affinity magnetic microspheres can fully utilize the sample enrichment effect thereof, and compared with agarose microspheres and polymer microspheres, can separate and purify hirudin with higher activity and purity, has a long storage period and stable quality, can provide safe and high-quality raw materials for health food, medicine or cosmetics, and is beneficial to industrialized production and application.

The invention relates to construction and an application technology of blood-glucose-reducing and thrombus-resisting lactic acid bacteria (HAL). 5 copies of long-acting insulin enterotropin (rolGLP-1) are respectively and seamlessly connected and fused with nattokinase (NK) and hirudin (HV) in series to obtain the blood-glucose-reducing antithrombotic peptide gene (HAP), and the product has dual functions of preventing and treating diabetes and thrombus complications thereof. A food-grade lactic acid bacteria HAP secretory expression vector pMG36-LacZ-Usp45-5rolGLP-1-NK (and-HV) is created by using beta galactosidase selection markers, and MG1363 competent cells are transformed by using the food-grade lactic acid bacteria HAP secretory expression vector so as to obtain lactic acid bacteria HAL1 (secretory expression insulinotropin-nattokinase) and HAL-2 (secretory expression insulinotropin-hirudin) which are stable in heredity and efficiently secrete HAP. Through intragastric administration of HAL samples to mouse thrombus and diabetes models, it is proved that HAL has good blood glucose reducing and thrombus resisting effects. The invention can be used for developing difunctional dietary therapy type blood glucose-reducing and thrombus-resisting lactic acid bacteria health-care products and beverages for simultaneously treating diabetes and thrombus complications thereof.

The invention relates to the technical field of traditional Chinese medicinal material processing, in particular to a leech fresh-cut processing device and method. Comprising the steps that a conveying mechanism is installed on a workbench so as to transversely fix and convey leeches, and an intelligent air control guiding-in mechanism and a fresh cutting mechanism conduct section limiting cutting on the leeches in a channel; and the fresh cutting mechanism is connected with the controller, identifies the position of the leech based on the laser signal as a starting signal, controls the conveying mechanism to stop in a delayed manner so as to adjust the length of the feed opening of the leech, and is linked to cut the leech in a node limiting manner. Impurities are removed from the source, the relative content of hirudin and other antithrombotic polypeptides is remarkably increased, the color and smell of a finished product are improved, the risk of pathogenic bacteria is reduced, efficient and standardized production is achieved through automatic equipment, and the problems of component dilution, quality degradation, low efficiency and the like existing in a traditional processing mode are solved.

The invention discloses a natural hirudin extraction method and application, and relates to the technical field of natural hirudin extraction.The method is characterized by comprising the steps that a tissue structure capable of being subjected to salivary gland single cell combined gland culture is selected; washing away bloodstain in the tissue structure by using PBS, then washing for three times by using the antibacterial liquid, and finally washing away the residual antibacterial liquid by using PBS; inoculating the tissue structure to a cell culture plate, placing the cell culture plate in a 28 DEG C incubator for inverted culture for 2 hours, then adding a DMEM / F-12 complete medium containing a growth additive and not containing serum, and placing the cell culture plate in the 28 DEG C incubator; when the tissue structure generates a large number of secretory vesicles, collecting the culture solution, adding a new culture solution, taking the collected culture solution as a natural hirudin crude product, and separating and purifying the natural hirudin crude product to obtain the natural hirudin. The method can achieve the effects of long-term survival in vitro and generation of a large amount of secretions.

The invention provides a multi-target synergistic cardiovascular protection beverage which comprises functional components and auxiliary components, and the functional components comprise nattokinase, hirudin, squalene, EPA, DHA and phytosterol; the preparation method of the multi-target synergistic cardiovascular protection beverage comprises the following steps: S1, firstly, washing the functional components with deionized water respectively to remove surface impurities, dust and microorganisms for subsequent processing; compared with the prior art, the raw material drying device has the following beneficial effects that in the drying process, flowing of hot air can take away moisture on the surfaces of raw materials, the humidity gradient is formed, the drying process is more uniform, and the phenomenon that the raw materials are locally overheated or dried unevenly is avoided; the method disclosed by the invention has the following beneficial effects that the raw materials are washed by deionized water and are dried by vacuum or low-temperature hot air, so that impurities and microorganisms can be thoroughly removed, and the loss of heat-sensitive functional components can be reduced.

The invention provides a hirudin penetration-promoting herbal compound body fat health-care liquid as well as a preparation method and application thereof, and the hirudin penetration-promoting herbal compound body fat health-care liquid comprises the following raw materials in percentage by weight: 0.3-0.6% of a leech extract, 0.3-0.6% of a Chinese herbal medicine extract and the balance of water. 5-10% of a herbal compound extract; 0.3 to 0.5 percent of methyl salicylate; 0.2 to 0.4 percent of ginger root oil; 8-12% of ethanol; and the balance of water. Wherein the herbal compound extract comprises an aloe vera extract, an astragalus membraneus extract, a ginseng extract and a panax notoginseng leaf and stem extract. According to the invention, a three-in-one technical system of efficient permeation promotion, multi-element conditioning, stability and mildness is constructed, the technical bottlenecks of low transdermal efficiency, single effect and insufficient stability of a traditional external herbal product are broken through, and the synergistic gain of body fat conditioning and skin repair is realized.

The invention provides a high-activity hirudin freeze-drying preparation as well as a preparation method and application thereof, and the preparation method comprises the following steps: (1) introducing a hirudin gene to a chassis cell to construct a recombinant engineering strain; (2) inoculating the engineering strain into a prepared culture medium, carrying out fermentation culture at a set temperature, after the thallus density reaches a target density, switching to methanol induced expression, and after continuous induction is carried out for a preset time, extracting a fermentation liquid; (3) performing ultrafiltration concentration and chromatographic purification on the obtained fermentation liquor to obtain a purified hirudin solution; and (4) adding a stabilizer into the obtained hirudin solution, pre-freezing to completely cure the hirudin solution, and drying to obtain the target hirudin freeze-dried preparation. According to the invention, the high-activity, high-purity, high-stability and low-cost industrial production of the hirudin freeze-drying preparation is realized through collaborative optimization of genetic engineering strain construction, high-density fermentation, multi-step purification and freeze-drying processes.

The invention relates to the field of leech breeding, and particularly discloses a device and method for increasing the hirudin content of leeches based on a natural secretion method. According to the leech feeding device, the feeding part, the anti-blocking assembly and the floating ball are arranged to form the leech feeding device, the feeding slurry is contained in the loading pipe, then the loading pipe is wrapped with the casing to avoid leakage of the feeding slurry, and after the loading pipe and the floating ball are thrown into a leech culture pond, the floating ball can float on the water surface; the loading pipe is vertically suspended in the water tank through the connecting line and the connecting buckle, the leeches can be adsorbed on the casing to suck the feeding slurry in the loading pipe, animal skin tissues are simulated through the casing, the leeches are induced to secrete hirudin in the sucking process, and then the hirudin secreting capacity of the bred leeches is exercised and improved; and the anti-blocking assembly can prevent the solid part in the feeding slurry from blocking the leaking holes, and it is guaranteed that the leeches can smoothly suck the feeding slurry in the loading pipe.

The invention relates to the technical field of biological medicines, and particularly discloses a hirudin-containing composition and application thereof in preparation of a product with a scalp absorption improving effect. The hirudin-containing composition is prepared from hirudin and a traditional Chinese medicine extract, the traditional Chinese medicine extract is prepared from the following raw materials: herba ecliptae, cacumen biotae and rhizoma drynariae. Researches show that the composition has the effect of promoting hair follicle cell proliferation; in addition, the absorption on the scalp can be improved; the important application value is realized.

The invention belongs to the technical field of traditional Chinese medicine pharmacy, and particularly relates to poecilobdella manillensis freeze-dried powder as well as a preparation method and application thereof. The preparation method of the poecilobdella manillensis freeze-dried powder comprises the following steps: (1) taking living poecilobdella manillensis or freezing and melting the living poecilobdella manillensis, and homogenizing to obtain a poecilobdella manillensis sample solution; (2) carrying out water extraction on the poecilobdella manillensis sample liquid; (3) performing fat extraction on the poecilobdella manillensis precipitate subjected to water extraction; and (4) combining the two extracts to prepare freeze-dried powder. The hirudin content of the poecilobdella manillensis freeze-dried powder prepared by combining a water extraction method with a fat extraction method is remarkably higher than that of a commercially available poecilobdella manillensis product, and the poecilobdella manillensis freeze-dried powder has a remarkable effect on treating thrombotic diseases and other vascular diseases.

The present invention relates to the technical field of hirudin extraction, and proposes a device and method for extracting leech salivary gland secretions, including a recovery box, a skin fluid collection box and a saliva collection box, wherein the recovery box is provided with a skin fluid recovery chamber and a saliva recovery chamber, the bottom of the skin fluid collection box is connected to the skin fluid recovery chamber via a connecting pipe, and the saliva collection box is connected to the saliva recovery chamber via a discharge pipe. The present invention can extract skin gland fluid while performing saliva-assisted extraction by setting a skin fluid collection box and a saliva collection box, firstly utilizing the characteristics of leeches that like quietness and avoid light, so that the skin gland secretions are scraped off when the leeches drill into the saliva collection box, and then by feeding an inducer and automatically pressing the leeches to vomit the saliva secretions, the integrated collection method is convenient to use.

The disclosure relates to the technical field of dietary nutritional supplements, in particular to a hirudin formula composition with anticoagulant and hypolipidemic effects and a preparation method thereof. Technical problem: the hirudin formula composition with anticoagulant and hypolipidemic effects and the preparation method thereof are intended to solve the technical problems that dietary nutritional supplements in the prior art cannot achieve anticoagulant and hypolipidemic effects. Technical solution: a hirudin formula composition with anticoagulant and hypolipidemic effects includes the following components by weight: 50 mg to 500 mg of hirudin polypeptide, 10 mg to 100 mg of red yeast rice, 10 mg to 100 mg of nattokinase, 10 mg to 100 mg of sulforaphane, 10 mg to 100 mg of agaricus blazei murill, 10 mg to 100 mg of quercetin, 10 mg to 300 mg of hirudin, and 50 mg to 300 mg of panax notoginseng extract.

The invention relates to the technical field of medicine detection, and provides a blood coagulation quality control kit and a preparation method thereof, a detection solution of 1L of the blood coagulation quality control kit comprises the following components in concentration: 110.17-185.26 g / L of nicotinamide, 80-150 mM / L of trihydroxymethyl aminomethane, 1.02-3.17 g / L of a hirudin solution, 15-30 g / L of a stabilizer, 0.5-2.8 ml / L of a preservative, 20-50 g / L of an excipient and the balance of a solvent, and the solvent is normal human plasma. Compared with the prior art, the method has the advantages that the stability is improved, the application range is wide, the method can be suitable for coagulation tests of five items including PT, APTT, TT, FIB and AT III, the values of the coagulation tests of the five items including PT, APTT, TT, FIB and AT III are within coagulation indexes of normal people, and the quality control requirements of the coagulation tests of the five items including PT, APTT, TT, FIB and AT III are met.

The utility model belongs to the technical field of hirudin extraction, and particularly relates to an extraction device for hirudin, which comprises an extraction box. Living leeches are placed in a filter box, then a top cover is fixed, a driving motor drives an eccentric wheel to rotate, the eccentric wheel extrudes a ball when rotating for the first half circle, the ball drives an L-shaped plate to move rightwards, the L-shaped plate drives a connecting plate to slide on a guide rod and compress a first spring, and the connecting plate drives the filter box to move rightwards. When the eccentric wheel rotates to the rear half circle, extrusion on the ball is gradually released, the first spring is reset, the first spring drives the filter box to move leftwards through the connecting plate, the filter box shakes left and right in a reciprocating mode, then the leeches are stimulated to automatically spit saliva, and then the saliva of the leeches is flushed to the bottom of the extraction box through the filter box by spraying water through a spray head; when hirudin is separated out in water, shaking of the filtering box is achieved, the leeches are stimulated to secrete saliva more actively, and therefore the extraction period is shortened.

The invention discloses a hirudin and pseudo-ginseng compound and application thereof, the hirudin and pseudo-ginseng compound comprises hirudin and rare ginsenoside from pseudo-ginseng, and the weight ratio of the hirudin to the rare ginsenoside from pseudo-ginseng is 1: 0.1-2: 1; the hirudin and pseudo-ginseng compound is applied to a preparation for improving skin microcirculation, and the preparation comprises the hirudin and pseudo-ginseng compound and at least one pharmaceutically acceptable auxiliary material. The invention has the following beneficial effects: skin microcirculation is improved; the anti-inflammatory effect is obvious; the synergistic interaction effect is achieved.