43 results about "Hirudin" patented technology

The invention provides an extraction method of hirudin, an oral preparation containing the hirudin and a preparation method of the oral preparation, and belongs to the technical field of leech processing and traditional Chinese medicine. The hirudin extraction method comprises the following steps: S1, soaking fresh leeches in a freeze-drying protective agent, taking out the leeches, wiping the surfaces of the leeches, pre-freezing the leeches at the temperature of 40 DEG C below zero to 50 DEG C below zero for 1-2 hours, and freeze-drying the leeches at the temperature of 20 DEG C below zero to 30 DEG C below zero for 4-6 hours to obtain freeze-dried leeches; s2, crushing the freeze-dried leeches, adding the crushed leeches into water, adding enzyme and lecithin, performing enzymolysis for 4-6 hours under the conditions that the temperature is 35-40 DEG C and the pH value is 7.5-8.5, and cooling to room temperature to obtain an enzymolysis product; s3, sterilization, ultrafiltration and drying are conducted, and hirudin is obtained. The hirudin extracted by the method is high in activity and high in recovery rate.

The invention discloses a fusion protein rHTA and application thereof, the fusion protein rHTA is sequentially composed of a Hirudin protein, a thrombin cleavage site and an AnxV protein from the N terminal to the C terminal, and the amino acid sequence is as shown in SEQ ID NO: 4 from the 1st site to the 393th site from the N terminal. Experiments prove that the fusion protein rHTA is specifically activated by thrombin at the local part of thrombus and releases AnxV protein and Hirudin protein, and the AnxV protein can specifically recognize and combine with PS, so that the fusion protein rHTA is enriched at the thrombosis part containing platelets in a targeted manner, the Hirudin protein is locally released under the action of thrombin, local precise anticoagulation is realized, and the anticoagulation efficiency is further enhanced. Therefore, the fusion protein rHTA provided by the invention not only has high anticoagulant activity, but also realizes enrichment and release at the thrombus formation part, and reduces the bleeding risk. The method has an important application value.

This invention relates to the field of health products or food technology, and more specifically to a composition and its application in removing dampness and controlling blood sugar, balancing blood pressure, and regulating blood lipids. The invention provides a composition for removing dampness and controlling blood sugar, balancing blood pressure, and regulating blood lipids, comprising Poria cocos extract, Astragalus membranaceus extract, hirudin, and at least one or more of Eucommia ulmoides extract, Gynostemma pentaphyllum extract, and Morus alba twig extract. The composition provided by this invention utilizes extracts from at least four precious Chinese medicinal herbs to exert a synergistic effect, jointly achieving the effects of lowering blood pressure, removing dampness and lowering blood sugar, and lowering blood lipids.

The invention discloses a hirudin escherichia coli engineering bacterium with controllable production generations as well as a construction method and application thereof, and relates to the technical field of biological medicines. The invention discloses a construction method of hirudin escherichia coli engineering bacteria with controllable production generations, which comprises the following steps: constructing a hokC protein gene as shown in the sequence of SEQ ID NO.1 and a constitutive promoter trxA as shown in the sequence of SEQ ID NO.2 into a recombinant expression vector pET28a (+)-ptrxA-hokC, and then transforming the recombinant expression vector into a hirudin synthetic strain E. coli Hirudin HV1-3 to obtain the hirudin escherichia coli engineering bacteria with controllable production generations. Through multiple rounds of culture, the hirudin yield of the engineering strain is reduced to 0 in the tenth round. The method is suitable for various hirudin types, and has the characteristics of reliable operation and high safety.

The invention discloses a recombinant leech polypeptide with high anticoagulant activity as well as a preparation method and application thereof. A PelB-T2Y-N47K-N52Y mutant is constructed by carrying out structural optimization on a hirudin HV2 sequence, a polypeptide product with relatively high anticoagulant activity is obtained after induced expression and nickel affinity chromatography purification are carried out in a prokaryotic expression system, and the anticoagulant activity of the polypeptide product reaches 9135.8 ATU / mg and is remarkably superior to that of an initial sequence HV2. Meanwhile, the polypeptide shows a lower IC50 value in a hydroxyl radical scavenging experiment, and has the activity of inhibiting generation of AGEs in a non-enzymatic saccharification model. Molecular docking and binding free energy analysis show that the combination of the mutant and thrombin (PDB ID: 4HTC) is more stable. The leech polypeptide disclosed by the invention has the potential of being developed into a multifunctional bioactive peptide preparation, and is suitable for the related fields of anticoagulation, antioxidation and anti-saccharification.

The invention relates to an exosome with high permeability and application of the exosome, and the surface of the exosome is connected with dioleoyl phosphatidylserine, 1, 2-dioleoyl-sn-glycerol-3-phosphorylethanolamine and hirudin. The exosome constructed by the invention can improve the permeability of the skin, can carry active components to directly reach the skin epidermis and corium layer, is accumulated on the epidermis and corium layer, continuously provides nutrition for the skin, and fully exerts the skin care and beautifying effects.

The invention provides a hirudin separation and purification method and system. The method comprises the following steps: grinding leeches into slurry; adding trypsin into the serous fluid, and then carrying out hydrolysis; performing counter-current extraction on the enzymatic hydrolysate obtained after hydrolysis; carrying out centrifugal separation on extract obtained by counter-current extraction, and collecting supernate; performing ultrafiltration concentration on the supernate by using an ultrafiltration membrane to obtain a concentrated solution; adsorbing hirudin in the concentrated solution by using DEAE-Sepharose FF resin, eluting, and then collecting an eluent; and carrying out vacuum freeze drying on the eluent to obtain freeze-dried powder. The system comprises a purification tank, a grinding device, an enzymolysis tank, a counter-current extraction device, a centrifugal machine, an ultrafiltration concentrator, an ion exchange chromatography instrument and a freeze dryer. In the hirudin production process, conditions are mild, damage to heat-sensitive components is small, high-toxicity organic solvents are not used, green and environment-friendly effects are achieved, and the prepared hirudin is high in activity retention rate and purity.

The invention belongs to the technical field of nano preparations, and particularly relates to a hirudin nano drug for relieving chronic asthma and a preparation method thereof.The hirudin nano drug is prepared from, by weight, 0.5-1 part of hirudin prodrug, 10-15 parts of stabilizer, 40-80 parts of freeze-drying protective additive and 1.5 parts of histidine; the hirudin prodrug molecule is composed of hirudin, MMP-9 responsive peptide, ROS responsive thioether bond and hydrophobic hexadecanoic acid, can spontaneously assemble to form a nano-sized prodrug under hydrophilic and hydrophobic effects, has stability in systemic circulation and is targeted and enriched at an asthma airway inflammation part; the hirudin with the thrombin activity inhibition effect is responsively released at the focus, so that the effect and the purpose of slowly treating the asthma are achieved.

Provided are a method for extracting a hirudin mutant, HV2-Lys47, from a fermentation broth of the hirudin mutant HV2-Lys47 produced by the fermentation of Escherichia coli, and purifying same, the use of the hirudin mutant HV2-Lys47 in an anticoagulated blood collection tube, and an anticoagulated blood collection tube containing the hirudin mutant HV2-Lys47. In the purification method, a membrane technology and a primary column chromatography technology are used to obtain the hirudin mutant HV2-Lys47.

The present application belongs to the technical field of biological medicine separation and purification, and particularly relates to a leech-derived hirudin separation and purification method based on thrombin affinity magnetic microspheres and application thereof. The present application uses self-prepared leaching liquor to coarsely extract hirudin from leech powder, then selects carboxyl magnetic microspheres to prepare thrombin affinity magnetic microspheres, and further separates and purifies by using the thrombin affinity magnetic microspheres, so as to finally obtain natural hirudin with an activity of 17000 ATU / g or more. The self-prepared leaching liquor can destroy the cell wall of leech, so that the hirudin in the leech is fully dissolved, thereby providing higher efficiency for the coarse extraction of hirudin in leech powder. The prepared thrombin affinity magnetic microspheres can fully utilize the sample enrichment effect thereof, and compared with agarose microspheres and polymer microspheres, can separate and purify hirudin with higher activity and purity, has a long storage period and stable quality, can provide safe and high-quality raw materials for health food, medicine or cosmetics, and is beneficial to industrialized production and application.

The invention relates to the technical field of traditional Chinese medicinal material processing, in particular to a leech fresh-cut processing device and method. Comprising the steps that a conveying mechanism is installed on a workbench so as to transversely fix and convey leeches, and an intelligent air control guiding-in mechanism and a fresh cutting mechanism conduct section limiting cutting on the leeches in a channel; and the fresh cutting mechanism is connected with the controller, identifies the position of the leech based on the laser signal as a starting signal, controls the conveying mechanism to stop in a delayed manner so as to adjust the length of the feed opening of the leech, and is linked to cut the leech in a node limiting manner. Impurities are removed from the source, the relative content of hirudin and other antithrombotic polypeptides is remarkably increased, the color and smell of a finished product are improved, the risk of pathogenic bacteria is reduced, efficient and standardized production is achieved through automatic equipment, and the problems of component dilution, quality degradation, low efficiency and the like existing in a traditional processing mode are solved.

The invention discloses a natural hirudin extraction method and application, and relates to the technical field of natural hirudin extraction.The method is characterized by comprising the steps that a tissue structure capable of being subjected to salivary gland single cell combined gland culture is selected; washing away bloodstain in the tissue structure by using PBS, then washing for three times by using the antibacterial liquid, and finally washing away the residual antibacterial liquid by using PBS; inoculating the tissue structure to a cell culture plate, placing the cell culture plate in a 28 DEG C incubator for inverted culture for 2 hours, then adding a DMEM / F-12 complete medium containing a growth additive and not containing serum, and placing the cell culture plate in the 28 DEG C incubator; when the tissue structure generates a large number of secretory vesicles, collecting the culture solution, adding a new culture solution, taking the collected culture solution as a natural hirudin crude product, and separating and purifying the natural hirudin crude product to obtain the natural hirudin. The method can achieve the effects of long-term survival in vitro and generation of a large amount of secretions.

The invention provides a multi-target synergistic cardiovascular protection beverage which comprises functional components and auxiliary components, and the functional components comprise nattokinase, hirudin, squalene, EPA, DHA and phytosterol; the preparation method of the multi-target synergistic cardiovascular protection beverage comprises the following steps: S1, firstly, washing the functional components with deionized water respectively to remove surface impurities, dust and microorganisms for subsequent processing; compared with the prior art, the raw material drying device has the following beneficial effects that in the drying process, flowing of hot air can take away moisture on the surfaces of raw materials, the humidity gradient is formed, the drying process is more uniform, and the phenomenon that the raw materials are locally overheated or dried unevenly is avoided; the method disclosed by the invention has the following beneficial effects that the raw materials are washed by deionized water and are dried by vacuum or low-temperature hot air, so that impurities and microorganisms can be thoroughly removed, and the loss of heat-sensitive functional components can be reduced.

The invention provides a hirudin penetration-promoting herbal compound body fat health-care liquid as well as a preparation method and application thereof, and the hirudin penetration-promoting herbal compound body fat health-care liquid comprises the following raw materials in percentage by weight: 0.3-0.6% of a leech extract, 0.3-0.6% of a Chinese herbal medicine extract and the balance of water. 5-10% of a herbal compound extract; 0.3 to 0.5 percent of methyl salicylate; 0.2 to 0.4 percent of ginger root oil; 8-12% of ethanol; and the balance of water. Wherein the herbal compound extract comprises an aloe vera extract, an astragalus membraneus extract, a ginseng extract and a panax notoginseng leaf and stem extract. According to the invention, a three-in-one technical system of efficient permeation promotion, multi-element conditioning, stability and mildness is constructed, the technical bottlenecks of low transdermal efficiency, single effect and insufficient stability of a traditional external herbal product are broken through, and the synergistic gain of body fat conditioning and skin repair is realized.

The invention provides a high-activity hirudin freeze-drying preparation as well as a preparation method and application thereof, and the preparation method comprises the following steps: (1) introducing a hirudin gene to a chassis cell to construct a recombinant engineering strain; (2) inoculating the engineering strain into a prepared culture medium, carrying out fermentation culture at a set temperature, after the thallus density reaches a target density, switching to methanol induced expression, and after continuous induction is carried out for a preset time, extracting a fermentation liquid; (3) performing ultrafiltration concentration and chromatographic purification on the obtained fermentation liquor to obtain a purified hirudin solution; and (4) adding a stabilizer into the obtained hirudin solution, pre-freezing to completely cure the hirudin solution, and drying to obtain the target hirudin freeze-dried preparation. According to the invention, the high-activity, high-purity, high-stability and low-cost industrial production of the hirudin freeze-drying preparation is realized through collaborative optimization of genetic engineering strain construction, high-density fermentation, multi-step purification and freeze-drying processes.

The invention relates to the technical field of biological medicines, and particularly discloses a hirudin-containing composition and application thereof in preparation of a product with a scalp absorption improving effect. The hirudin-containing composition is prepared from hirudin and a traditional Chinese medicine extract, the traditional Chinese medicine extract is prepared from the following raw materials: herba ecliptae, cacumen biotae and rhizoma drynariae. Researches show that the composition has the effect of promoting hair follicle cell proliferation; in addition, the absorption on the scalp can be improved; the important application value is realized.

The invention belongs to the technical field of traditional Chinese medicine pharmacy, and particularly relates to poecilobdella manillensis freeze-dried powder as well as a preparation method and application thereof. The preparation method of the poecilobdella manillensis freeze-dried powder comprises the following steps: (1) taking living poecilobdella manillensis or freezing and melting the living poecilobdella manillensis, and homogenizing to obtain a poecilobdella manillensis sample solution; (2) carrying out water extraction on the poecilobdella manillensis sample liquid; (3) performing fat extraction on the poecilobdella manillensis precipitate subjected to water extraction; and (4) combining the two extracts to prepare freeze-dried powder. The hirudin content of the poecilobdella manillensis freeze-dried powder prepared by combining a water extraction method with a fat extraction method is remarkably higher than that of a commercially available poecilobdella manillensis product, and the poecilobdella manillensis freeze-dried powder has a remarkable effect on treating thrombotic diseases and other vascular diseases.

The disclosure relates to the technical field of dietary nutritional supplements, in particular to a hirudin formula composition with anticoagulant and hypolipidemic effects and a preparation method thereof. Technical problem: the hirudin formula composition with anticoagulant and hypolipidemic effects and the preparation method thereof are intended to solve the technical problems that dietary nutritional supplements in the prior art cannot achieve anticoagulant and hypolipidemic effects. Technical solution: a hirudin formula composition with anticoagulant and hypolipidemic effects includes the following components by weight: 50 mg to 500 mg of hirudin polypeptide, 10 mg to 100 mg of red yeast rice, 10 mg to 100 mg of nattokinase, 10 mg to 100 mg of sulforaphane, 10 mg to 100 mg of agaricus blazei murill, 10 mg to 100 mg of quercetin, 10 mg to 300 mg of hirudin, and 50 mg to 300 mg of panax notoginseng extract.

The utility model belongs to the technical field of hirudin extraction, and particularly relates to an extraction device for hirudin, which comprises an extraction box. Living leeches are placed in a filter box, then a top cover is fixed, a driving motor drives an eccentric wheel to rotate, the eccentric wheel extrudes a ball when rotating for the first half circle, the ball drives an L-shaped plate to move rightwards, the L-shaped plate drives a connecting plate to slide on a guide rod and compress a first spring, and the connecting plate drives the filter box to move rightwards. When the eccentric wheel rotates to the rear half circle, extrusion on the ball is gradually released, the first spring is reset, the first spring drives the filter box to move leftwards through the connecting plate, the filter box shakes left and right in a reciprocating mode, then the leeches are stimulated to automatically spit saliva, and then the saliva of the leeches is flushed to the bottom of the extraction box through the filter box by spraying water through a spray head; when hirudin is separated out in water, shaking of the filtering box is achieved, the leeches are stimulated to secrete saliva more actively, and therefore the extraction period is shortened.

The application provides an extraction method of hirudin, an oral preparation containing the hirudin and a preparation method of the oral preparation, and belongs to the technical fields of hirudo processing and traditional Chinese medicine. The extraction method of the hirudin comprises the following steps: S1, soaking fresh hirudo in a freeze-drying protective agent, wiping the surface after taking out, pre-freezing at-40 to-50 DEG C for 1 to 2 hours, and then freeze-drying at-20 to-30 DEG C for 4 to 6 hours to obtain freeze-dried hirudo; S2, crushing the freeze-dried hirudo, adding water, adding enzymes and lecithin, and enzymolysis at 35 to 40 DEG C and pH=7.5 to 8.5 for 4 to 6 hours, and then cooling to room temperature to obtain an enzymolysis product; and S3, sterilizing, ultrafiltering and drying to obtain the hirudin. The hirudin obtained by the method has high activity and high recovery rate.

The invention discloses a method for efficiently extracting hirudin from poecilobdella manillensis living bodies, and belongs to the technical field of hirudin separation and extraction. The method comprises the following steps: after hunger treatment of poecilobdella manillensis, inducing secretion by combining an induction solution containing malic acid, citric acid, calcium racemic ketoisoleucine and other components with a pig blood extract, and centrifuging to obtain a crude extract; the method comprises the following steps: preparing an immobilized microsphere carrier through L-cysteine functional modification and amino cationic starch crosslinking, immobilizing thrombin, mixing with a crude extract, extracting, eluting, and freeze-drying to obtain a hirudin product. By optimizing an induced secretion system and an immobilized adsorption purification process, the problems of low secretion induction efficiency, complex purification and insufficient activity retention rate in a traditional method are solved, in-vivo repeated extraction of hirudin is realized, and the method has the advantages of high extraction efficiency, high product purity, high specific activity, reutilization of leech living bodies and the like, and is suitable for industrial production. The large-scale production requirement can be met.

The invention discloses a hirudin separation and purification method based on affinity chromatography, and belongs to the technical field of hirudin separation and purification. Aiming at the characteristics of high viscosity and many impure proteins of a hirudin crude extract, an affinity chromatography medium polystyrene microsphere is modified: modified methoxy polyethylene glycol-polyaspartic acid containing 3, 5-di-tert-butyl-4-hydroxycinnamic acid is grafted, so that the hydrophilicity is improved, the non-specific adsorption is reduced, and the affinity chromatography efficiency is improved; meanwhile, free radicals are removed by utilizing a hindered phenol structure so as to protect the activity of thrombin; after hydroformylation treatment, fixing thrombin as a ligand to form a stable affinity medium; and combining with thrombin affinity chromatography separation, and then carrying out ultrafiltration concentration, desalination and freeze drying to obtain hirudin. A macroporous structure is reserved through a modified medium, the mass transfer resistance is reduced, and the binding efficiency of hirudin and ligands is improved; the activity loss of the hirudin is reduced in a subsequent process. The method can efficiently separate and purify hirudin, has good adsorption selectivity and stability, and is suitable for large-scale separation.

The invention provides a PMP hollow fiber membrane with an antithrombotic function and a preparation method of the PMP hollow fiber membrane. According to the method, a decahydronaphthalene / xylene low-temperature solvent system is constructed, PMP is uniformly dissolved under the condition that the temperature does not exceed 90 DEG C, and a thermal protective agent is adopted, so that the biological activity of hirudin in the processing process is effectively maintained; furthermore, oxidized chitosan is used as a controlled release carrier, and high load and slow release regulation of hirudin are realized through directional covalent coupling grafting of the zwitterionic polymer; finally, the physical barrier of the zwitterionic polymer and the direct anticoagulation effect of the hirudin are combined to construct a synergistic antithrombotic mechanism. On the premise of not depending on heparin, the obtained hollow fiber membrane has the comprehensive advantages that the blood coagulation time is remarkably prolonged, hirudin is released stably and durably, and HIT risks are avoided, is suitable for clinical scenes such as blood purification and in-vitro oxygenation, and has important clinical application value.

The invention discloses preparation and application of oxygen-triggered gelation spray for diabetes wound treatment, and belongs to the technical field of functional materials. The invention provides an oxygen-triggered gelation hydrogel system containing hirudin and a preparation method of the oxygen-triggered gelation hydrogel system. The hydrogel is prepared from tyramine modified hyaluronic acid (HA-Tyr), glucose oxidase (GOD), horse radish peroxidase (HRP) and glucose (Glu). The components of the hydrogel are kept in a solution state in the spray bottle (after oxygen is removed). After the solution is sprayed, glucose oxidase reacts with glucose in the presence of oxygen to generate hydrogen peroxide (H2O2). The generated H2O2 triggers horse radish peroxidase to catalyze HA-Tyr crosslinking, and the hydrogel is formed. The hydrogel is applied through spraying, is more tightly attached to the surface of a wound, can better play the effects of resisting bacteria, improving microcirculation and reducing the inflammation level, and has remarkable potential in the aspect of treating the chronic diabetes mellitus wound.

This invention discloses an antithrombotic medical coating, its preparation method, and its application. The antithrombotic medical coating is prepared by mixing an aqueous resin, hirudin-loaded PLGA / CS microspheres, a dispersant, a wetting agent, a pH adjuster, a film-forming aid, a foaming agent, and deionized water. The hirudin-loaded PLGA / CS microspheres are drug-loaded microspheres with a double-shell core-shell structure, consisting of hirudin as the core, PLGA as the inner shell, and CS as the outer shell. The antithrombotic medical coating can be used to prepare an antithrombotic coating containing microneedles. The prepared microneedle-loaded coating not only has the advantages of active permeation promotion, intelligent sustained release, and comfortable breathability, but also achieves non-invasive, long-lasting, convenient, and comfortable antithrombotic prevention functions, significantly improving adherence to antithrombotic treatment. Furthermore, it is adaptable to various flexible substrates, easily manufactured into various wearable antithrombotic products, and offers flexible application options. It also solves the common problems of brittle fracture and low yield in the preparation of microneedle patches.

The application provides a kind of palace pill for clearing palace and removing blood stasis and a preparation method thereof, which is prepared from the following raw materials in parts by weight: 25-30 parts of wine red peony root, 15-20 parts of vinegar-fried aconite, 15-20 parts of bran-fried broad wood, 15-20 parts of fried five spirit resins, 15-20 parts of vinegar-fried corydalis, 10-15 parts of hirudin, 5-10 parts of earthworm protein peptide powder and 5-10 parts of oyster peptide powder. The preparation steps of the oyster peptide powder are as follows: fresh oysters are selected, washed with running water, cleaned of internal organs and mucus, soaked in a citric acid solution, ultrasonically broken, enzymatically hydrolyzed, deodorized, purified, filtered, concentrated and dried to obtain oyster peptide powder. The palace pill has a scientific formula, a synergistic effect of various components, the efficacy of clearing palace and removing blood stasis, heat-clearing and detoxifying, carbuncle-dissolving and pus-discharging, blood stasis-removing and pain-stopping, is convenient to carry and take, and has clinical drug value.

1. The name of the design product: mask packaging box (hirudin tricholoma face mask). 2. The use of the design product: used for containing and packaging single piece packaged mask. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best indicates the design points: perspective view 1.

The invention relates to the technical field of biological medicine manufacturing, in particular to a traditional Chinese medicine compound gel containing bioactive organic components and capable of synergistically repairing hypertrophic scars based on four-season qi and a preparation method of the traditional Chinese medicine compound gel. The compound gel is formed by mixing a bottle A base matrix and a bottle B four-season additive according to the ratio of 9: 1, the base substrate of the bottle A contains various bioactive organic components such as a salvia miltiorrhiza extract, asiatic acid, bioactive hirudin, perilla oil and the like; according to the four-season additive in the bottle B, corresponding traditional Chinese medicine extracts are selected according to seasons, the preparation method adopts a biotechnology process including supercritical COextraction, a biocompatibility nanometer co-grinding technology and other modern biotechnologies, the transdermal absorption rate of mineral components is remarkably increased, the anti-fibrosis effect is enhanced through a hirudin-perilla oil soluble synergistic system, and the anti-fibrosis effect is improved. The four-season differentiated formula realizes accurate repair of preventing wind evil in spring, clearing blood heat in summer, moistening dryness injury in autumn and dredging collaterals in winter.

This invention discloses a small molecule peptide derived from *Hirudo medicinalis*, with the amino acid sequence Leu-Leu-Phe-Arg. The peptide was obtained by enzymatically hydrolyzing the waste protein residue after purifying hirudin from *Hirudo medicinalis*, combined with tyrosinase-functionalized magnetic bead affinity fishing technology and mass spectrometry identification. In vitro kinetics and molecular dynamics simulations confirmed that the peptide LLFR significantly prolongs the lag time of monophenolase reactions. It penetrates the catalytic pocket of tyrosinase through an "induced fit" mechanism, forming a stable dead-end complex with the enzyme and inducing the unfolding of the enzyme's tertiary structure. Cell experiments confirmed that LLFR significantly inhibits tyrosinase activity and melanin synthesis in melanoma cells without significant cytotoxicity. This peptide exhibits high safety and good water solubility, providing a new approach for the high-value utilization of industrial waste from *Hirudo medicinalis*, and is suitable for the industrial production of whitening cosmetics and functional products.

The invention is applicable to the technical field of hirudin extraction, and provides a method for extracting and separating hirudin series products from living leeches, which comprises the following steps: feeding adult poecilobdella manillensis subjected to fasting starvation treatment with an inducer, adding a small molecule emetic to promote emesis, and collecting culture water to obtain a natural hirudin crude product; the method comprises the following steps: filtering by using a bag filter to remove fine particles, effectively separating hirudin from components larger than hirudin and components smaller than hirudin by using ultrafiltration membranes with different molecular weights, and hydrolyzing protein larger than hirudin in molecular weight by using neutral protease or alkaline protease; the leeches are extracted and separated to obtain various products such as hirudin, small-molecule oligopeptide and small-molecule extracts. According to the method, the culture pond is directly used for induction, emetic and separation, hirudin is effectively separated from macromolecules and micromolecules, combination can be carried out according to market requirements, and multi-variety and multi-effect leech series products are obtained.