Technique for producing hirudin by using poecilobdella manillensis frozen fresh body as raw material

A production process and a technology of hirudo are applied in the field of production technology for extracting hirudin, and can solve the problems of low yield and high activity of hirudin

Inactive Publication Date: 2008-07-30
GUANGDONG OCEAN UNIVERSITY
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Problems solved by technology

The hirudin obtained by this method has high activity and good medicinal eff...

Method used

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  • Technique for producing hirudin by using poecilobdella manillensis frozen fresh body as raw material

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Embodiment Construction

[0006] The present invention realizes through the following measures:

[0007] 1) After thawing the frozen (-18°C) fresh body of Hirudo phenanthae, put it into a tissue grinder and grind it into a slurry. Add 2 times of 0.15mol / L sodium chloride solution according to the mass of the slurry, and stir at 240r / min for 1 hour. Filter through a 120-mesh nylon mesh and collect the filtrate. Put the solid matter into a tissue masher and mash it into a slurry, extract it twice according to the previous operation, and then filter it. The solids were discarded and the filtrate was collected. The three filtrates were combined, boiled and rapidly cooled to room temperature to obtain suspension 1.

[0008] 2) Suspension 1 was filtered with a 200-mesh nylon mesh, and the filtrate was collected. Add an equivalent amount of 0.15mol / L sodium chloride solution to the solid matter according to the mass of the frozen fresh body of B. phenanthrene, and stir at 180r / min for 1 hour. Filter thro...

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Abstract

The invention relates to a hirudin production process that a Hirudinaria manillensis refrigerated fresh body is taken as raw material, which belongs to the pharmaceutical field of the traditional Chinese medicine, and relates to the active constituent extraction of animals. The invention aims at solving the problem that the traditional hirudin extraction process is difficult to be applied to the industrialized production. The process adopts the method that the hirudin is extracted from the whole Hirudinaria manillensis, thus the industrialized production can be operated. The Hirudinaria manillensis is rectified, and the pulp is distilled for three times with normal saline, and filtered. The filtrate is boiled and filtered, and protein is removed from the filtrate. The filtrate is collected, and after freezing, concentration and alcoholization, and the hirudin crude product is obtained. The hirudin crude product is washed with the normal saline, and then washing solution is concentrated, and finally the product is obtained through freezing and drying. The production process method of the hirudin is simple, and the quality of the end product is qualified. The pharmacology experiment result indicates that the hirudin extracted by utilizing the process has good pharmacological action.

Description

technical field [0001] The process relates to the field of traditional Chinese medicine pharmacy, in particular to a production process for extracting hirudin from frozen fresh Hirudo phenanthae. Background technique [0002] my country has abundant leech resources that can be utilized. Leech is a commonly used drug for promoting blood circulation and removing blood stasis in traditional Chinese medicine, and many Chinese patent medicines based on leeches are popular in the market. But this kind of medicine is inconvenient to use, and the curative effect is not obvious. [0003] The leech used in this process is Hirudo phenanthrene, a blood-sucking leech of the genus Hirudophyllidae, which is widely distributed in Hainan, Guangdong, Guangxi, Fujian and Yunnan in my country, and is one of the pests in the local rice fields. The clinical application of Hirudo phenanthae not only provides effective drugs for the prevention and treatment of cardiovascular diseases, but also el...

Claims

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Application Information

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IPC IPC(8): C07K1/14C07K2/00
Inventor 苗艳丽宋文东方富永李思东彭少伟范润珍黄甫谷长生林宏图覃亮
Owner GUANGDONG OCEAN UNIVERSITY
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