The application discloses a method for preparing and removing
odor of
Hirudo nipponia freeze-dried
powder, and the method comprises the following steps: inducing clean treatment in an empty
stomach, low-
temperature stress pretreatment, in-situ
quick freezing under the protection of
inert gas, segmented vacuum freeze-
drying and low-temperature
airflow crushing. The application induces the expression up-regulation of
aldehyde dehydrogenase and
alcohol dehydrogenase in the body of
Hirudo nipponia through low-
temperature stress, and utilizes
ice crystals to destroy
cell compartmentalization in in-situ
quick freezing, so that the enzymes and their substrates are contacted, in-situ enzymatic conversion occurs under the protection of
inert gas, and fishy components are degraded into low-smell metabolites. The application does not need exogenous
odor-removing substances and post-
processing procedures, has high
antithrombin activity
retention rate, greatly reduces the total amount of fishy substances, and realizes in-situ synergistic
odor removal in the whole freeze-
drying process.