52 results about "Antithrombin Activity" patented technology

The invention discloses a reagent for detecting antithrombin activity, including reagent 1 and reagent 2, wherein: reagent 1 is a chromogenic substrate of FXa, and reagent 2 is a diluent containing FXa and heparin; wherein the chromogenic substrate of FXa is selected from From the following: CH ₃ SO ₂ ‑D‑Leu‑Gly‑Arg‑p‑nitroanilide·AcOH; CH ₃ OCO‑D‑CHG‑Gly‑Arg‑p‑nitroanilide·AcOH; CH ₃ OCO‑D‑CHA‑Gly‑Arg‑p‑nitroanilide AcOH; Benzoyl‑Ile‑Glu‑Gly‑Arg‑p‑nitroanilide HCl; Suc‑Ile‑Glu(γ‑Piperidyl)‑Gly‑Arg‑p‑nitroanilide HCl; N-α-Z-D-Arg-Gly-Arg-p-nitroanilide 2HCl; Boc-D-Arg-Gly-Arg-p-nitroanilide 2HCl; Acetyl-D-Arg-Gly-Arg-p ‑nitroanilide·2HCl; 4‑Nz‑D‑Arg‑Gly‑Arg‑p‑nitroanilide·2HCl; 4‑Mbs‑D‑Arg‑Gly‑Arg‑p‑nitroanilide·2HCl. The invention has good detection stability and repeatability, high sensitivity and accuracy, can accurately reflect the activity of antithrombin, and can find patients with abnormal antithrombin activity in the body, thereby providing a basis for treatment.

The invention discloses a leech culture method and belongs to the technical field of aquaculture. The method specifically comprises the following steps: (1) building an aquaculture cage; (2) arranging a culture pond; (3) putting in young leeches; (4) performing feeding management; and (5) performing water quality management. According to the method disclosed by the invention, the yield and quality of leeches can be effectively improved, the yield can be improved by about 30%, the activity of antithrombin in the leeches is well enhanced, and the application value is high.

The invention relates to a method for extracting hirudin by stimulating a living leech with electricity. By utilizing the simulating effect of current on organism glands, a way for simulating a living leech to extract high-purity hirudin at mass by direct weak current is directly used, and the living character of the leech is maintained, therefore, the production process of hirudin is simplified,the product purity is improved, the wild leech resources are recycled, the production cost is reduced and the problem of seasonal disorder of production is solved. The hirudin extracted by using the method is subjected to nanofiltration water removal and decompression spray drying to be made into powdery hirudin with the antithrombin activity of 800AT-U/g and liquid hirudin with the antithrombin activity of 1,000AT-U/g when protective agents are added in the liquid hirudin.

The invention discloses a breeding method for fast increasing the activity of antithrombase of leeches. The breeding method comprises the following steps of 1 young leech collection, 2 weight-increasing breeding, 3 temperature control and feed deprivation and 4 harvesting. The breeding method for fast increasing the activity of the antithrombase of the leeches is short in breeding period, the activity of the antithrombase with unit mass of the leeches can be fast improved in the short term, the activity of the antithrombase with unit mass of the leeches can be improved by 35.28% to 116.61% within one month, the medicinal value of the leeches is effectively improved, the temporary keeping time is shortened, production management cost is reduced, breeding risks are reduced, and therefore the economic benefits of breeding can be increased.

The invention relates to a process for preparing high activity thrombin inhibitors, which comprises subjecting hirudin, the natural specific inhibitor of thrombin to DNA family restructuring, carrying out restructuring to hirudin HV1, HV2, HV3 genes, constructing reorganization library for hirudin bacteriophage display, using thrombin as coating antigen, subjecting bacteriophage reorganization library to three rounds of enriched elutriation including adsorption, elution and expansion, obtaining a plurality of bacteriophage monoclons with high affinity with thrombin through ELISA screening, carrying out pronucleus induced expression to these clones, finally detecting the antithrombogenic activity of the purification expression product.

The invention discloses a preparation method of an enteric coated tablet with leech as a raw material, belonging to the technical field of traditional Chinese medicine. In the invention, research is carried out on the freezing temperature, the number of days of freezing, a mashing process, a low-temperature drying process and a sterilizing process, and the antithrombin activity is used an indicator to optimize a new process method. The process method is organic combination of different process parameters, i.e. the process method avoids high mechanical force and high temperature to achieve the effect of maintaining the activity of leech. By the process method provided by the invention, the enteric coated tablet prepared by using complete fresh live leech as medicine has higher antithrombin activity.

The invention pertains to the field of medical technology, relating to an oxazine compound and a medical application thereof; the oxazine compound and an oxazine salt obtained from an addition reaction of pharmaceutically-applicable oxazine acid can be used as a platelet aggregation inhibitor; the oxazine compound has a structural formula shown as above; in a structural formula I and a structural formula II, X can be independently chosen from CH2 and C=0, R1 can be independently chosen from H and a substituted or un-substituted aromatic base, R2 can be independently chosen from H or a substituted or un-substituted aromatic base, and R3 can be independently chosen from H, alkyl group with one to four carbon atoms, benzyl or substituted or un-substituted aromatic base. The oxazine compound has simple synthetic method, adapts to industrialized production and is more stable when compared with natural analogues. Shown by biological activity assay, the oxazine compound has the functions of analgesia, anti-inflammatory and antithrombin activity and is a platelet aggregation inhibiting medicament.

The invention discloses a maixuekang capsule detection method. A maixuekang capsule is obtained through filling crushed fresh leech to a capsule. The detection method comprises methods for determining the contents of amino acids and polysaccharides in the maixuekang capsule, and makes up the single antithrombase activity determination and content determination methods in the prior art. The detection method has the advantages of accuracy, high sensitivity, good repeatability, and effective control of the quality of the maixuekang capsule.

The present invention provides compositions and methods for preventing blood-sucking infection in livestock, isolated protein with antithrombin activity and nucleotide sequence encoding the protein. A protein named thrombostatin was isolated from the salivary gland of horn fly western horn fly. The composition can be used as a veterinary vaccine to prevent infective horn flies from sucking blood on domestic animals. The proteins of the invention are also useful in the treatment of thrombosis.

The invention provides an antithrombin activity assay method of an injection for promoting blood circulation to remove blood stasis. The antithrombin activity assay method is characterized in that a ceramic whiteware spot plate is taken as a reaction carrier; thrombin titration interval time, thrombin concentration and stirring frequency of a tool pin are controlled to ensure that the determination results are stable. The antithrombin activity assay method provides a biological quality control method for the injection for promoting blood circulation to remove blood stasis, and facilitates evaluation to reliability and controllability of the quality.

The invention belongs to the field of biotechnology and relates to DTI (Direct thrombin inhibitor) peptides and the application thereof. According to the invention, the molecule of RGD-Hirudin is truncated on the basis of the structure of the RGD-Hirudin, so as to obtain micro-molecule DTI Peptide 1 and micro-molecule DTI Peptide 2. A test and an animal experiment show that the Peptide 1 and the Peptide 2 are DTIs, have antithrombin activity, have definite action target points, have no impact on other blood coagulation factors, can be further used for preparing oral anticoagulant drugs or subcutaneous sustained-release anticoagulant drugs, and particularly suitable for thrombotic disease prevention. The DTI peptides can be taken as candidate drugs for DTIs to be studied intensively, and lay the foundation for developing novel DTIs.

The present invention relates to preparation process of leech medicine granule. The preparation process includes preparing scalded leech piece, decoction, filtering, decompression concentrating the filtrate to obtain extractum, spray drying to obtain powder, mixing with magnesium stearate and one of dextrin, lactose and maltodextrin in certain weight proportion, and pelletizing with roller to form 18-40 mesh granule. The leech medicine granule contains amino nitrogen not less than 10 mg/g, active antitrombase not less than 16.0 U/g and sarkin not less than 2 mg/g.

The invention discloses a test box for rapidly detecting the activity of antithrombin of leeches. The test box comprises a 0.9 percent sodium chloride solution, a trihydroxymethyl aminomethane hydrochloric acid buffering solution containing 0.5 percent fibrinogen and a prepared thrombin solution. The invention further discloses a detection method for rapidly detecting the activity of the antithrombin of the leeches; the detection method comprises the following steps: 1) weighing and chopping the leaches and putting the leaches into the sodium chloride solution; 2) extracting and centrifuging;3) adding the trihydroxymethyl aminomethane hydrochloric acid buffering solution; 4) dropwise adding the prepared thrombin solution; 5) calculating; 6) calibrating according to environment temperature. The test box and the detection method can be used for rapidly detecting the activity of the antithrombin in the leeches, have the advantages of accurate detection result, low equipment requirementsand rapid detection, and can be suitable for outdoor on-site operation.

The invention discloses a making method of rice-processed leech, comprising soaking, segmenting and stir frying and characterized in that the soaking step is rice milk soaking, including: crushing glutinous rice and polished round-grained rice into powder, adding drinking water, boiling, cooling, and soaking leech; the stir frying step includes: stirring the soaked leech with glutinous rice and polished round-grained rice, and removing the two types of rice. The leech making method is modified; viscous fluid on the surface of leech is effectively removed; fishy smell of the leech is significantly relieved; stimulation to human stomach and intestine is significantly relieved; antithrombin activity of leech is improved.

The invention provides a method capable of quickly increasing leech antithrombase active content. The method is simple and convenient in operation and can obtain high-quality leech medicinal materials. By adopting the method, overall antithrombase total content of leech is maximum in the 5th day after sucking, which is increased by 49.5%, 73.5%, 69.1% and 126.0% when compared with that in the 1stday, the 3rd day, the 7th day and the 11th day after sucking. By applying the method, antithrombase activity of the leech is improved remarkably without increasing production cost, and leech medicinalmaterial quality is improved.

It is an object of the present invention to provide a therapeutically effective agent for therapy and/or improvement of DIC, or a method for treating and/or improving DIC. The present invention provides an agent for therapy and/or improvement of disseminated intravascular coagulation comprising thrombomodulin as an active ingredient, which is administered to antithrombin-reduced patients having a low plasma antithrombin activity of less than 50%.

The invention discloses a preparation method of an active extract gel product taking Hirudinaria Manillensis freeze-dried powder as a raw material. The method includes cleaning all fresh living Hirudinaria Manillensis, homogenizing, and freeze-drying to obtain freeze-dried powder; adding appropriate amount of pure water into the freeze-dried powder, stirring for extraction, filtering, collecting a filtrate, adding appropriate amount of pure water into residues, stirring for extraction, filtering, collecting the filtrate, and mixing the filtrate; adding appropriate amount of anhydrous ethanol into the filtrate, centrifuging, and collecting a supernatant as an active extractive solution for later use; and preparing carbopol gel matrix, adding sodium hydroxide to adjust pH value, slowly adding the active extractive solution into the prepared gel matrix, and stirring to obtain 20-40 antithrombin active units/g gel. The freeze-dried powder extract of Hirudinaria manillensis has very high activity, so that the extract can be made into cosmetics with high activity to ensure good effect; in addition, the extract is configured into cosmetics with different active units; and the gel product is convenient to manufacture, use and carry, and can further be applied locally and on the whole face as required.

The invention relates to the technical field of hirudo nipponia culture, in particular to a culture method for rapidly increasing antithrombin activity of hirudo nipponia. The culture method comprisesthe following steps that S1, a breeding water tank is arranged, wherein the length of the breeding water tank ranges from 100 cm to 200 cm, the width of the breeding water tank ranges from 40 cm to 60 cm, the height of the breeding water tank ranges from 30 cm to 40 cm, the water temperature in the water tank is controlled to range from 17 DEG C to 20 DEG C, and the water level in the water tankis controlled to range from 1 cm to 3 cm; S2, breeding seedlings of the hirudo nipponia; S3, conducting feed preparation; S4, feeding feed; and S5, conducting water quality management. The invention aims to provide the culture method for rapidly increasing the antithrombin activity of the hirudo nipponia. The culture method is used for solving the problems of low survival rate and low antithrombinactivity efficiency of the hirudo nipponia in the prior art.