1430 results about "Protective Agents" patented technology

The invention is applicable to the technical field of biological detection, provides a fluorescence immunochromatography detection method based on monatomic nano-enzyme enhancement, and particularly relates to two application forms: in the first form, a detection signal enhanced fluorescence immunochromatography detection card and a sample diluent containing a monatomic nano-enzyme are involved; in the second form, the invention relates to a fluorescence immunochromatography detection card with enhanced detection signals, and a sample pad of the detection card is obtained by soaking a sample pad treatment solution containing a monatomic nano-enzyme solution. The two application forms both relate to a monatomic nano-enzyme solution, and the solution is composed of monatomic nano-enzyme, bio-enzyme composite microspheres, a protective agent, a surfactant, a thickening agent and a preservative. The monatomic nano enzyme is composed of an atomic-scale dispersed metal active center and a carrier material. When the method is used for detecting myocardial and inflammatory markers, the limit of quantitation can reach the pg / mL level, the detection linear range can span three orders of magnitude, and the clinical examination use requirements can be met. The kit can be applied to rapid quantitative detection of myocardial and inflammatory markers, has the advantages of high sensitivity, rapidness, convenience and high efficiency, and provides an auxiliary diagnosis basis for early screening of myocardial infarction, heart failure and infection related diseases.

The invention belongs to the field of biology, and discloses a CRISPR / Cas12a freeze-dried microsphere, which is characterized in that the CRISPR / Cas12a freeze-dried microsphere is obtained by freeze-drying the following raw materials: enzyme-free water, 10 * HOLMES Buffer for Cas12a, an RNA enzyme inhibitor, CRISPR / Cas12a protein, crRNA, an upstream primer F, a downstream primer R, a fluorescent probe Reporter and a freeze-drying protective additive; the CRISPR / Cas12a protein is an LbaCas12a protein, and the CRISPR / Cas12a protein is a Cas12a protein; the freeze-drying protective agent is prepared from mannitol, trehalose, polyethylene glycol, glucan and BSA (Bovine Serum Albumin). By further optimizing a freeze-drying protective agent, freeze-drying microballing of a CRISPR / Cas12a system is achieved, the freeze-drying microballing can be matched with RPA constant-temperature amplification double microballing, and detection is conducted through a one-step method. Meanwhile, the invention further discloses a three-microsphere detection system, application and a kit.

The invention relates to the technical field of metal material hot working, and discloses a hot-rolled coiled plate production process which comprises the following steps: preparing a reaction type protective agent, the high-temperature-resistant coating is prepared from the following raw materials in parts by weight: 20 to 40 parts of tetraethoxysilane, 5 to 15 parts of triethyl phosphate, 1 to 5 parts of triethyl borate, 0.5 to 2.0 parts of cerous nitrate (III) hexahydrate, 0.1 to 1.0 part of lithium acetate and 0.01 to 0.05 part of vanadyl acetylacetonate (IV). Coating the surface of a hot-rolled plate blank with the protective agent; the coated plate blank is heated in a heating furnace, so that the protective agent forms a molten-state protective layer in situ; carrying out hot rolling on the plate blank with the molten protective layer; and cooling and coiling the hot-rolled steel strip. In the whole heating and hot rolling process, the molten protective layer isolates the contact between the steel and the oxidizing atmosphere, so that the generation of oxide scales is fundamentally inhibited, the production process is simplified, and the metal yield is increased.

The invention discloses a bacillus coagulans feed additive and a preparation method thereof, and relates to the technical field of feed additives, the bacillus coagulans feed additive comprises bacillus coagulans spores, the content of the spores is 1.0 * 10 < 8 >-1.0 * 10 < 11 > CFU / g; the protective agent system comprises the following components: maltodextrin or lactose, vegetable protein, polyhydric alcohol and an antioxidant; the invention discloses a microcapsule coating structure. A microcapsule is a two-layer composite coating of an inner layer alginate matrix and an outer layer polycation or polymer coating. According to the invention, bacillus coagulans spores which can still keep high survival rate under short-time high-temperature and low-pH conditions are obtained through screening and fermentation strategies of strains with high sporulation and heat and acid tolerance, the problem that common strains are easy to inactivate in granulation, extrusion and gastric acid environments is solved, the problem of product failure caused by insufficient tolerance of the strains is reduced, and the product quality is improved. And the original biological activity basis is improved, so that the protection effect of the subsequent formula and the coating can be played at a higher starting point, and the zoology effect of the final preparation is improved.

The invention discloses a biological challenge device with combined adjustable resistance for evaluating the effect of a sterilization process, which comprises a shell, the shell comprises a plastic pipe and an inner pipe cap detachably connected with the inner wall of the plastic pipe, the inner pipe cap is provided with a breathable window and covered by a breathable film, and a bacterial contamination carrier for bearing indicator biological spores is attached to the inner bottom of the plastic pipe. An openable container containing a liquid culture medium is arranged in the plastic pipe cavity; the outer side of the inner pipe cap is sleeved with an outer pipe cap detachably connected with the outer wall of the plastic pipe, and the outer pipe cap is provided with a flow blocking structure used for adjusting the speed of the sterilizing agent entering the area of the inner pipe cap; the indicator biological spores are pretreated by a stable protective agent containing one or more of polyethylene glycol, trehalose, sorbitol, polyvinylpyrrolidone and glyceryl stearate. The speed of a sterilizing agent entering the device can be limited, the spores are dry in a suspension added with a stable protective agent, the capability of resisting general environmental changes is very good, and the performance of the device can be maintained for a long time.

The invention relates to the technical field of fertilizers and pesticides for agricultural production, and discloses a liquid pesticide fertilizer for preventing and treating root-knot nematodes in a water-fertilizer integrated system and application thereof, and the liquid pesticide fertilizer is prepared from the following components: a plant source nematicide, a biocontrol inoculant, a macroelement and medium trace element fertilizer, a boron cross-linking agent, a modified polycarboxylic acid dispersing agent, a microbial protective agent and the like. The preparation method comprises the steps of biological phase pretreatment, matrix phase construction, oil phase micro-emulsification, multiphase fusion and dynamic crosslinking. Through trehalose gradient permeation pretreatment, the survival rate of biocontrol bacteria in a high-salt matrix is increased; a boric acid ester bond dynamic cross-linked network is constructed by using a variable pH adjusting process, so that the system is endowed with shear thinning characteristics, and the contradiction between suspension stability and drip irrigation fluidity is solved; and the chelating dispersion effect of the modified polycarboxylic acid dispersing agent is matched, so that the generation of precipitates is effectively inhibited, and stable coexistence and synergistic interaction of the pesticide, the fertilizer and the bacteria are realized.

The invention belongs to the technical field of mixing, and particularly relates to a mixing device and method for flue corrosion protection agent production, the mixing device comprises a mixing barrel and an inner barrel fixedly arranged in the mixing barrel, a cover plate is fixedly arranged on the mixing barrel, a rotating barrel is rotationally arranged on the inner barrel in a sleeving mode, and tooth protrusions are arranged on the rotating barrel; magnetic strips with alternating polarities are fixedly arranged in the rotating barrel in an array manner; wherein a spline sleeve shaft of the telescopic rotating mechanism is combined with a reciprocating groove of a sleeve and a convex rod of an embedded assembly, so that the telescopic mixing paddle can be driven to do composite motion of rotating and lifting, and meanwhile, a movable plate of the telescopic mixing paddle does horizontal reciprocating sliding under the action of attraction force and repulsive force of a magnetic strip and a magnetic block and an elastic telescopic rod, so that three-dimensional composite stirring is formed; the rubber scraping plate moves along with the moving plate, raw materials attached to the inner wall of the inner barrel can be intermittently scraped off, caking of the raw materials is avoided, and when the mounting base synchronously moves along with the moving plate, the inclined sawteeth on the mounting base can effectively cut powder balls in the raw materials.

The invention provides a cyclic peptide used for treating, improving or preventing nervous system injury of mammals or diseases or pains caused by the injury, neurodegenerative diseases, anxiety or epilepsy or used as a neuronal protective agent, a conjugate containing the cyclic peptide, a pharmaceutical composition containing the cyclic peptide or the conjugate and application of the cyclic peptide and the conjugate.

The invention discloses a QS-21 composite adjuvant and a preparation method thereof, and belongs to the technical field of biological medicines. According to the QS-21 composite adjuvant, a polyanion material and QS-21 are compounded to form nanoparticles, and the nanoparticles are organically combined with a freeze-drying protective additive, so that the prepared QS-21 composite adjuvant is relatively high in retention rate, the problems of hydrolysis and oxidation are solved, the requirement of a phospholipid carrier is avoided, and a vaccine co-preparation process is simplified; the subsequently added freeze-drying protective agent retains the immune enhancement activity of the QS-21 and reduces the exposure of the QS-21 to a degradation environment; the corresponding cytotoxicity meets the standard, and the hemolysis rate is low; therefore, the method has remarkable clinical application value and industrialization potential.

The invention relates to a method for preparing high-molecular-weight polyethylene furandicarboxylate by a one-step method, and belongs to the technical field of bio-based polyester materials. The method comprises the following steps: weighing raw materials including 2, 5-furandicarboxylic acid, ethylene glycol, an end group regulator, a protective agent, a decarboxylation inhibitor and a catalyst, mixing and adding into a reaction container, introducing nitrogen, maintaining the pressure of the nitrogen in the reaction container to be 50-150 kPa, carrying out esterification reaction, heating and vacuumizing until the pressure is less than or equal to 50 Pa after the esterification reaction is completed, and carrying out polycondensation reaction to obtain the 2, 5-furandicarboxylic acid. And carrying out extrusion and drying so as to obtain the high-molecular-weight polyethylene furandicarboxylate, wherein the high-molecular-weight polyethylene furandicarboxylate is obtained. According to the method, the FDCA conversion rate is larger than or equal to 98%, the number-average molecular weight of the obtained product is larger than or equal to 32 kg / mol, no heavy metal residues exist, and the method is suitable for large-scale production of high-end bio-based packaging materials.

The invention discloses stem cell freeze-dried powder for promoting skin healing and a preparation method of the stem cell freeze-dried powder. The freeze-dried powder comprises an effective component, a freeze-drying protective agent group and a polymer protective agent group, the effective component is umbilical cord mesenchymal stem cell conditioned medium freeze-dried supernatant, the protective agent group is selected from one or more of cane sugar, trehalose, lactose and mannitol, and the polymer protective agent group is selected from one or more of polyvinylpyrrolidone (PVP) and glucan. The preparation method comprises the following steps: collection of a conditioned culture medium, preparation of a freeze-drying protective agent solution, mixing treatment, low-temperature pre-freezing and freeze-drying, collection and packaging of freeze-dried powder and the like. The method can effectively retain stem cell-derived active factors and enhance the stability and redissolvability of the freeze-dried powder, and is suitable for skin wound repair, regenerative medicine and other scenes.

The invention discloses a freeze-drying protective agent composition, a freeze-drying protective agent, application of the freeze-drying protective agent, a probiotic freeze-dried product and a preparation method of the probiotic freeze-dried product. The freeze-drying protective agent composition comprises the following components in parts by weight: 0.5-4 parts of tremella polysaccharide; the relative molecular weight of the tremella polysaccharide is 1 KDa to 10 KDa; 0.5 to 4 parts of disaccharide; and 0.5-2 parts of sugar alcohol. The freeze-drying protective agent composition and water are uniformly mixed and then sterilized to obtain the freeze-drying protective agent, and the freeze-drying protective agent is used for preparing probiotic freeze-dried products and can effectively ensure the activity and stability of probiotics.

The invention discloses a gold nanocluster electrogenerated chemiluminescence probe which is a multifunctional gold nanocluster obtained by taking a 2-sulfydryl-1, 3, 4-thiadiazole ligand as a reducing agent or a protective agent. Comprising a solid-phase probe with a gold cluster modified on an electrode; or the gold cluster is independent of the electrode and is a liquid-phase probe dissolved in the electrolyte during use. The obtained electrogenerated chemiluminescence gold nanocluster probe can generate an electrogenerated chemiluminescence signal under the condition of no co-reactant, and is relatively low in luminescence potential, high in quantum yield and good in biocompatibility.

A Solid Phase Peptide Synthesis (SPPS) device and method of using the same for manufacturing peptides is taught herein. The system comprises at least two reactors, each reactor including a quantity of SPPS resin. The reactors are positioned in series. A de-protecting agent is added to the first reactor and then transferred to the second and third reactors, in series, thereby operating to de-protect the protected N-group. Wash solvent is added to the first reactor and then transferred to the second and this operation repeated several times. Likewise, an amino acid activated ester solution is added, in series, to the first, second and third reactors, thereby operating to couple the amino acid to the de-protected N-group. Wash solvent is added to the first reactor and then transferred to the second and this operation repeated several times prior to the next cycle. The use of the reactors in series reduces the overall solvent required. Online LCMS is also used to monitor progress and identity of reactions happening within the solid phase resin particles.

The invention relates to the technical field of organic high-molecular compound synthesis, in particular to a modified ABS material diffusion plate and a preparation method thereof.The preparation method comprises the steps that dried ABS resin and acrylonitrile-butadiene-styrene copolymer are mixed to prepare a modified ABS base material; mixing organic silicon microspheres and nano barium sulfate to obtain a modified light diffusion agent; judging that the modified light diffusant is unqualified by comparing the refractive index absolute difference between the modified light diffusant and the modified ABS base material, and performing modification compensation; a titanate coupling agent is determined to be adopted for modification according to the content of inorganic particles of the modified light diffusion agent; mixing the modified light diffusant with an antioxidant system, an ultraviolet protective agent and a processing aid to obtain a functional additive; and mixing with a modified ABS (Acrylonitrile Butadiene Styrene) base material, and extruding by using a twin-screw extruder to Through multi-parameter feedback and accurate compensation control, the problems that the compatibility of the modified light diffusion agent and the base material is poor and the stability of the machining process is insufficient are effectively solved.

The invention discloses a gelatin microcarrier lysate as well as a preparation method and application thereof. The gelatin microcarrier lysis buffer comprises lyase, a cell protective agent, a cell energy metabolism protective agent, an antioxidant, a buffer solution and a chelating agent. The gelatin microcarrier lysate has extremely strong protectiveness to cells, abandons damaging trypsin and EDTA, and innovatively adds a cell protection combination of a cell protective agent L-arginine, a cell energy metabolism protective agent sodium pyruvate and an antioxidant vitamin C, so that the cell survival rate (gt; 98%) and the retention rate (gt; the method is high in cell yield, good in safety and wide in range.

The invention discloses a freeze-dried essence with a repairing effect and a preparation method, and belongs to the field of skin care products, and the freeze-dried essence is composed of freeze-dried powder and an independent solvent liquid. The freeze-dried powder contains 0.5-3 parts of palmitoyl oligopeptide, 3-8 parts of I-type collagen, 1-5 parts of III-type collagen, 0.1-1 part of chitosan, 1-3 parts of secondary variable-temperature fermentation liquor of betula platyphylla and 2-8 parts of a freeze-drying protective agent; the solvent liquid is prepared from 0.5 to 3 parts of hyaluronic acid and 0.05 to 0.5 part of sodium polyglutamate. The preparation method comprises the following steps: a, adding fermentation liquor after dissolving; b, adding nano liposome and a protective agent; c, quickly freezing to-55 + / -2 DEG C by liquid nitrogen, and preserving heat for 2.5-3.5 hours; d, drying for 9-11 hours under the vacuum of 15-25 Pa and at the temperature of-18 + / -2 DEG C; and e, the temperature is increased to 15 + / -2 DEG C in a stepped mode, vacuum is smaller than or equal to 20 Pa, and drying is conducted for 18- Through scientific formula design, multiple effects of efficient repairing, anti-aging and relieving are achieved.

The invention discloses a probiotic chitosan microsphere and a preparation method thereof, and belongs to the technical field of probiotic embedding, and the method comprises the following steps: dissolving trehalose and L-glutamine in water, suspending lactic acid bacteria in the water, mixing with a chitosan solution to obtain a mixed solution, dropwise adding the mixed solution into a sodium tripolyphosphate solution, and stirring to obtain the probiotic chitosan microsphere. And washing insoluble substances, and performing freeze drying to obtain the probiotic chitosan microspheres. The trehalose and the L-glutamine are used as protective agents and nutrient substances of the probiotics at the same time, can synergistically improve the survival rate of the probiotics, are matched with other components, comprehensively improve the tolerance and activity of the probiotics, and are combined with a specific curing means and freeze drying, so that damage of high temperature to the probiotics can be avoided; the obtained microspheres have good probiotic protection capability, stability and appropriate release performance, can effectively improve the survival rate of the probiotics in storage and application processes, and can better play roles in improving intestinal flora and improving the health level of hosts.

This invention discloses a pH-sensitive curcumin nanoliposome, its preparation method, and its applications. The preparation method includes the following steps: dissolving 1,2-dioleoyl-SN-glycerol-3-phosphoethanolamine, cholesterol succinate monoester, cholesterol, pH-sensitive material, and curcumin in an alcohol solvent to obtain an inner-phase alcohol solution; dissolving a lyophilization protectant in water to obtain an outer-phase aqueous solution; the inner-phase alcohol solution is compressed by the outer-phase aqueous solution to form a laminar flow and diffuses into the aqueous phase to form a narrow mixed solvent region. When the ethanol content in the mixed solvent region is lower than the ethanol content required for the original substances to dissolve in the ethanol phase, the lipids will self-assemble to form liposomes. The liposomes prepared by this invention have a spherical morphology, controllable size, and sustained-release properties in vitro, showing good application prospects as anti-tumor drug carriers in the field of drug delivery.

The invention discloses a nasal delivery multistage targeting bionic nano drug delivery system as well as a preparation method and application of the nasal delivery multistage targeting bionic nano drug delivery system. According to the drug delivery system, a black phosphorus nanosheet with an active oxygen scavenging effect is used as an inner core carrier, a neuroprotective agent fingolimod hydrochloride FTY720 and polydimethyldiguanide PolyMet are loaded, and a microcolloid cell membrane and a mitochondrial membrane are modified on the surface of the preparation; the nano drug delivery system is applied to treatment of cerebral arterial thrombosis reperfusion injury, can overcome the blood brain barrier, can quickly enter the brain through nasal administration, realizes efficient delivery of drugs, remarkably reduces the cerebral infarction area, reduces the brain inflammation level, protects nerve cells, greatly relieves cerebral arterial thrombosis reperfusion injury, and has application prospects.

The invention provides a purification method of mesenchymal stem cell exosomes, prepared mesenchymal stem cell exosome particles and application of the mesenchymal stem cell exosome particles, and belongs to the technical field of exosomes. The preparation method comprises the following steps: performing multiplication culture on adipose-derived stem cells to stimulate exosome secretion, then performing gradient centrifugation, collecting supernate, adding a protective agent, uniformly stirring and mixing, adding a polymer, stirring and incubating, centrifuging, collecting exosome particles, washing, and freeze-drying to obtain the mesenchymal stem cell exosome particles. The mesenchymal stem cell exosome particle prepared by the invention is simple in preparation method, relatively low in cost, easy to realize industrial application and high in yield, meanwhile, the mesenchymal stem cell exosome particle has a very good protection effect on exosomes, the preservation period of the exosomes is prolonged, and the prepared mesenchymal stem cell exosome particle has the characteristic of releasing the exosomes in a sustained and controlled release manner and has a good application prospect. The traditional Chinese medicine composition is widely applied to preparation of anti-inflammatory, anti-oxidation and skin repair promoting medicines.

Provided are compositions containing a conjugate containing a phthalocyanine dye, including compositions containing stabilizing agents, such as non-ionic surfactants and / or protectants. In some aspects, the compositions result reduced aggregation of the conjugate due to agitation, temperature exposure, and / or pH. Also provided are articles of manufacture containing the compositions containing the conjugates, and methods for their administration to subjects for photoimmunotherapy. In some embodiments, the phthalocyanine dye conjugates are conjugated to a targeting molecule, such as an antibody, that targets the conjugate to a cell or pathogen, such as by binding to a cell surface protein.

2-diarylmethyl-4-aminotetrahydropyran derivatives are disclosed. The compounds include the following genus:or a pharmaceutically acceptable salt thereof. The compounds activate cellular PP2A, suppress oncogenic kinase signaling and negatively regulate MYC and MYCN in cancer. The compounds also induce FOXO transcription factor translocation to the nucleus by modulating PP2A and, as a consequence, exhibit anti-proliferative effects. They are useful in the treatment of a variety of disorders, including as a monotherapy in cancer treatment, or used in combination with other drugs to restore sensitivity to chemotherapy where resistance has developed.

The invention discloses an application and method of SAH-CIM hydrogel in promoting survival of retinal ganglion cells in glaucoma. The hydrogel comprises a Y-type primitive composed of three DNA chains and a siRNA connector, and the nucleotide sequences of the three DNA chains are SEQ ID NO.1-SEQ ID NO.3; the method comprises the following steps: S1, constructing a Y-type primitive: mixing three DNA chains with equal molar concentration in an annealing buffer solution, performing high-temperature denaturation at 95 DEG C, and then quickly cooling to 4 DEG C to finish annealing, so as to form a stable Y-type structure with three-way cohesive ends; s2, synthesizing a siRNA (small interfering Ribonucleic Acid) connector; and S3, mixing the Y-type element and the siRNA connector according to a molar ratio of 1: 1.5 at room temperature to react for 30 minutes, and forming a three-dimensional network structure through complementary pairing driven self-assembly, thereby finally obtaining the SAH-CIM hydrogel. The SAH-CIM hydrogel provided by the invention can be used for preventing / treating ischemia-reperfusion injury and related diseases, for example, the SAH-CIM hydrogel can be used for treating glaucoma, accurate co-delivery of a neuroprotective agent and an immunomodulator can be realized, and the SAH-CIM hydrogel has the advantages of treatment accuracy and biocompatibility.

The invention relates to an invar alloy mask plate plasma nanometer polishing and thinning system and method, the system comprises a water tank (2), the water tank (2) is filled with a polishing solution (3) and an invar alloy mask plate (4), and the depth of the invar alloy mask plate (4) in the water tank (2) is controlled through a lifting system controller (5) and a lifting device (6); the polishing solution (3) comprises main salt, auxiliary salt and water; the main salt comprises (conductive electrolyte for generating plasma) 1-2% of potassium citrate and 1-2% of potassium nitrate; the auxiliary salt comprises a buffering agent, a thickening agent, a protective agent and a brightening agent. Through the synergistic effect of a special polishing solution formula and an optimization process, efficient and uniform thinning and nanoscale polishing of the ultrathin invar alloy mask plate are achieved, the surface flatness and environmental protection property are improved while the natural color gloss of metal is guaranteed and edge burning and salt residue are avoided, and the high-precision use requirement of the mask plate is met.

The invention relates to the field of biological product preparation, in particular to a preparation method of high-activity oyster-derived small molecule peptide. The method comprises the following steps: selecting fresh meat of pacific oysters or ostrea rivularis as a raw material, degreasing, adding water, crushing, filtering, sequentially carrying out compound protease and neutral protease hydrolysis, implanting a sensor and a probe in hydrolysis, monitoring in real time, and dynamically adjusting reaction conditions; adding a targeting microcapsule wrapping an ascorbic acid glutathione composite protective agent, and releasing the protective agent when a target peptide signal is detected; separating and concentrating through a gradient membrane, and purifying the target peptide by using a molecular imprinting chromatographic column; carrying out alkaline hydrolysis on enzymatic hydrolysis residues to prepare chitosan for cyclic utilization, carrying out enzymatic hydrolysis on residual residues to generate collagen peptide, treating and recycling wastewater, and recovering protease. According to the method, the technical effects that the high-activity and high-purity target peptide is prepared, the resource utilization rate is increased, the production cost is reduced, the target peptide has good oxidation resistance and biological activity, the intestinal enzyme degradation rate is reduced after glycosylation modification, and the blood half-life period is prolonged are achieved.

The invention discloses a preparation method of a high-activity exosome hair growth promoter, which comprises the following steps: providing purified exosome, and mixing the purified exosome with hair growth active ingredients in a PBS (Phosphate Buffer Solution) with the pH value of 7.0-7.6, adding hydroxypropyl-beta-cyclodextrin with the final concentration of 0.5%-2% (w / v) and trehalose with the final concentration of 5%-10% (w / v), mildly incubating for 1-2 hours under the conditions that the temperature is 35-37 DEG C and the speed is 50-100 rpm, purifying, and freeze-drying to obtain a solid product. According to the invention, through the synergistic effect of the specific membrane permeability enhancer and the membrane stabilization protective agent, the efficient loading of the hair growth active component and the natural biological activity of the exosome are considered, the process is mild and controllable, the industrial amplification is easy, and the solid preparation solves the problem of inconvenient storage and transportation, and has strong practicability.

The invention discloses a self-emulsifying adjuvant of a vaccine and a preparation method of the self-emulsifying adjuvant, and relates to the technical field of vaccine adjuvants. The adjuvant comprises an oil phase, an emulsifier and a freeze-drying protective agent, the oil phase comprises squalene and tocopherol, the emulsifier comprises polysorbate and a polyoxyethylene-polyoxypropylene block copolymer, and the freeze-drying protective agent comprises sugar and sugar alcohol. The preparation method comprises the following steps: emulsifying an oil phase and a water phase to form a nano-emulsion, and then freeze-drying to obtain freeze-dried powder. The adjuvant has the advantages of small particle size, uniform distribution, high stability, rapid reconstruction and the like, and can effectively maintain active components and improve the immune effect of vaccines.

The invention relates to a gastric acid-resistant sustained-release intestinal bacteria capsule based on bionic mucous membrane-enzymolysis response and a preparation method thereof. The gastric acid-resistant sustained-release intestinal bacteria capsule comprises a core layer, an adhesive layer, an outer-layer composite film and a protective coating from inside to outside, the core layer consists of intestinal flora and a multifunctional freeze-drying protective agent; the adhesive layer is composed of trehalose, N-acetyl glucosamine and MUC2 recombinant protein; the outer-layer composite film is composed of a base material and a functional filler; the protective coating is composed of beewax and chitosan. The anti-gastric acid sustained-release intestinal bacteria capsule based on bionic mucosa-enzymolysis response provided by the invention has excellent gastric acid resistance, and the gastric acid resistance is remarkably improved; good slow release performance is achieved, and accurate slow release of enzymatic hydrolysis response can be achieved; the colonization efficiency of the intestinal bacteria can be effectively improved; the storage stability is good. The preparation method provided by the invention has the advantages of simplified steps, short time consumption, controllable core operation time and obviously shortened whole process, and is beneficial to keeping the activity of the flora.

The invention discloses preparation and application of a rabies virus G protein mRNA vaccine freeze-drying preparation, and relates to the field of veterinary biological products. According to the invention, rabies virus G protein mRNA is prepared on a large scale through an in-vitro transcription technology, and an mRNA-LNP (lipid nanoparticle) delivery system is successfully constructed by adopting a microfluidic encapsulation process. Through freeze-drying protective agent formula screening (including cane sugar, trehalose and mannitol) and freeze-drying procedure parameter optimization (including pre-freezing temperature, main drying rate and secondary drying residual moisture control), the freeze-dried mRNA vaccine preparation which can stably exist under the condition of 4 DEG C is finally obtained.