236 results about "Protective Agents" patented technology

The invention discloses a compound microorganism freeze-drying protective agent, a preparation method of the compound microorganism freeze-drying protective agent and a use method of the compound microorganism freeze-drying protective agent. Comprising the following raw materials in parts by weight: 0.03 part of disodium ethylene diamine tetraacetate, 5 to 15 parts of trehalose, 2 to 5 parts of sorbitol, 0.5 to 2 parts of ascorbic acid, 0.05 to 0.2 part of N-acetyl-L-cysteine, 0.1 to 0.5 part of polylactic acid-glycolic acid copolymer, 0.01 to 0.05 part of alpha-tocopherol and 1 to 3 parts of bentonite. The PLGA, the alpha-tocopherol and the NAC construct a three-stage synergistic anti-oxidation system from outside to inside: the PLGA is externally blocked, so that rapid consumption of an antioxidant is avoided, and slow release is realized; the alpha-tocopherol realizes oxidative damage repair of cell membranes; nAC maintains the internal reduction environment of cells, reduces oxidative stress and can regenerate alpha-tocopherol at the same time, and reutilization of resources is achieved.

The present application provides a kind of 3-aminoisobutyric acid derivatives and preparation method thereof.The 3-aminoisobutyric acid derivatives of the present application are formed by 3-aminoisobutyric acid and arginine, and the preparation method comprises: step A: Boc-L-BAIBA is condensed with guanidyl-protected L-arginine under the action of condensing agent to obtain fully-protected dipeptide;Step B: the fully-protected dipeptide is reacted under the action of deprotecting agent to obtain 3-aminoisobutyric acid derivative.

This invention belongs to the pharmaceutical field and discloses a rubitetidine pharmaceutical composition, its preparation method, and its application. The pharmaceutical composition includes rubitetidine, an organic acid, and additives, wherein: rubitetidine has crystal forms including crystal form C and / or crystal form D; the additives include a lyophilization protectant or a solution stabilizer; the lyophilization protectant is a saccharide compound and / or its derivative; and the solution stabilizer is an alcohol compound. This invention focuses on rubitetidine crystal form C and rubitetidine crystal form D, and prepares a lyophilized rubitetidine formulation by adding specific organic acids and lyophilization protectants. This formulation exhibits good solubility and stability. Furthermore, by adding specific organic acids and solution stabilizers, a rubitetidine injection solution with similarly good solubility and stability can be prepared without adding lyophilization protectants or using a lyophilization-free process, optimizing the process steps and solving the problems of long production cycles and cumbersome reconstitution operations associated with traditional products.

This invention relates to the field of biotechnology, specifically disclosing a protective agent composition for long-term preservation of Scutellaria baicalensis exosomes and its preparation method. The protective agent composition comprises an osmotic pressure regulator, a stabilizer, a buffer, and a solvent. By optimizing the ratio of each component, the stability of Scutellaria baicalensis exosomes at low temperatures is significantly improved, extending their active preservation time. This composition is suitable for applications in drug delivery, cosmetics, and disease treatment.

The application discloses a high-salt-resistant denitrifying bacterial strain and a microsphere preparation thereof, a preparation method and application thereof, relates to the technical field of environmental microorganisms and wastewater biological treatment. Pseudomonas sp. The classification name of the strain is Pseudomonas sp., the preservation number is CGMCC No. 36626, the strain is preserved in the China General Microbiological Culture Collection Center, and the preservation date is November 14, 2025. The preparation method of the microsphere preparation containing the strain comprises the following steps: carrying out strain amplification culture to obtain a bacterial liquid, mixing the bacterial liquid with porous starch and a protective agent, then performing vacuum freeze drying to obtain bacterial powder, and then mixing the bacterial powder with a sodium alginate solution, and dropping the mixture into a calcium chloride solution to obtain the microsphere preparation. The microsphere preparation can maintain a nitrate nitrogen removal rate of more than 80% after being stored at-18 to-22 DEG C or 20 to 25 DEG C for 28 days, and can be used for denitrification and nitrogen removal of low-carbon-nitrogen-ratio and high-salt wastewater (such as urine wastewater in a closed circulation system).

The present invention relates to insect neuropeptide compounds of the family of pyroglutamic peptides and analogs thereof having activity against insects, such as Hemiptera, Diptera, Lepidoptera, Blattaria and / or Coleoptera insects, such as aphids and fruit flies, and their use as insect control agents (e.g. insecticides) and plant protection agents.

Disclosed herein is deuterated buprenorphine as a protective agent for fetal subjects against full-agonist opioid exposure. Use of deuterated buprenorphine prevents or reduces exposure of the fetus to opioids used by the mother. Use of deuterated buprenorphine may prevent or reduces exposure of the fetus to harmful metabolites of buprenorphine.

The present application relates to a method for synthesizing leu-enkephalin by Fmoc method, which uses Fmoc-protected amino acid as monomer, and sequentially connects amino acid to resin. In the synthesis of leu-enkephalin, all the amino acids do not have any side chain protection group. The Fmoc protection group is removed by using a solution of 1.00 mol / L imidazole and 0.02 mol / L tricyclohexylphosphine in tetrahydrofuran, and finally leu-enkephalin is obtained by removing the resin with aqueous trifluoroacetic acid. The present application first uses imidazole and tricyclohexylphosphine as components of Fmoc deprotection agent, instead of traditional pyridine. The deprotection agent is not controlled by "controlled chemicals", is cheap and easy to obtain, is stable in nature, and is low in toxicity, volatility and corrosion. The deprotection agent has excellent Fmoc removal ability, does not cause phenolic ring acylation side reaction of tyrosine side chain, does not need any side chain protection, simplifies the production process of polypeptide, reduces the preparation cost of polypeptide, and provides great improvement space for the design of synthesis strategy of polypeptide containing basic sensitive side chain protection group.

Provided is a renal function maintenance and protection agent comprising as an active ingredient of a triglyceride of docosahexaenoic acid (DHA) or eicosapentaenoic acid (EPA) or a mixture of a triglyceride of docosahexaenoic acid (DHA) and a triglyceride of eicosapentaenoic acid (EPA), which can be used as a pharmaceutical or food composition.A triglyceride of docosahexaenoic acid (DHA) or eicosapentaenoic acid (EPA) or a mixture of a triglyceride of docosahexaenoic acid (DHA) and a triglyceride of eicosapentaenoic acid (EPA) is used as an active ingredient for renal function maintenance and protection agents.

The utility model discloses a kind of corrosiveness test devices of tobacco protective agent, including box and box cover, box cover is set on the box, the box includes outer shell and inner shell, inner shell is fixedly arranged in outer shell and vacuum heat insulation cavity is formed between inner shell side wall and outer shell side wall, heating body is provided in vacuum heat insulation cavity, sealing groove is provided in outer shell top, the bottom surface of box cover is provided with sealing ring and bottle mouth sealing plate for covering test bottle bottle mouth is arranged, sealing ring corresponds with sealing groove;The bottom of outer shell is connected with bottom plate by spring, vibration mechanism for driving the vibration of box is arranged on bottom plate, baffle is slidably arranged on the outside of bottom plate.The utility model aims at providing a kind of corrosiveness test devices of tobacco protective agent, to improve the sealing property and insulation room temperature of the device, prevent the volatilization of protective agent.

The application provides a buffer for improving the stability of foot-and-mouth disease vaccine antigen, which contains milk serum, sodium chloride, sorbitol, glycine, K2HPO4.3H2O and KH2PO4. The buffer has the effects of improving the thermal stability of 146S antigen and obviously inhibiting the degradation of 146S antigen under the conditions of 4 DEG C and 37 DEG C. The antigen resuspended by the buffer and the prepared vaccine can be stored for a long time under the condition of 4 DEG C. In addition, the buffer is easy to prepare and dissolve, can be used as a medium for resuspending FMD antigen, is a good thermal stability protective agent for FMD antigen and vaccine, can be used in large-scale production, has strong practicability, can obviously reduce the degradation rate of effective antigen in foot-and-mouth disease vaccine under storage conditions and extreme conditions, and obviously improves the product quality.

The present invention aims to provide a cytoprotective agent against ferroptosis that contains DHMBA as an active ingredient, by verifying the effect of DHMBA in suppressing ferroptotic cell death using DN model mice and human renal tubular cells HK-2, for each useful agent containing DHMBA as an active ingredient. [Solution] The present invention provides a cell agent for ferroptosis that has a cytoprotective effect against ferroptosis, comprising 3,5-dihydroxy-4-methoxybenzyl alcohol as an active ingredient.

This invention belongs to the field of traditional Chinese medicine fermentation technology, specifically involving a high-enzyme-active Liu Shen Qu (a type of fermented medicinal herb) and its fermentation process. The process first processes red beans, bitter almonds, artemisia annua, cocklebur, and polygonum hydropiper into a medicinal porridge, which is then mixed with wheat flour and a specific fermenting agent to form a soft mass. Its innovation lies in the use of a Bacillus subtilis trehalose protective agent complex and Aspergillus oryzae chitosan microspheres. These two fermenting agents are prepared by spray drying encapsulation and ionogel methods, respectively, significantly improving the survival rate and stability of the fermenting microorganisms during processing and storage. The soft mass undergoes two stages of precisely controlled temperature and humidity fermentation, and finally, after shaping and segmented drying, the finished product is obtained. This process effectively solves the problems of low enzyme activity and poor stability in traditional Liu Shen Qu fermentation, resulting in a Liu Shen Qu product with high digestive enzyme activity and stable, uniform quality.

The application discloses a lyophilized powder of Evf and a preparation method thereof. The lyophilized powder comprises recombinant human acidic fibroblast growth factor, excipient, protein protective agent, pH buffer and lyophilized preparation maintenance agent. The lyophilized powder prepared by the method has high stability and is not easy to be broken into powder in the shaking process, so that electrostatic is avoided due to the friction between the broken powder and the bottle wall in the storage and transportation process, and the product quality is improved.

Use of cyclodextrin and its derivatives as protective agent in preparation of TACI-Fc fusion protein liquid drug preparation. The use of cyclodextrin as a protective agent can improve the long-term stability of TACI-Fc fusion protein, maintain good biological activity, improve drug production efficiency and greatly improve the convenience of drug administration.

The application discloses a nucleic acid rapid release agent, a preparation method thereof and a nucleic acid rapid release method. The nucleic acid rapid release agent comprises Tris-HCl, EDTA, Triton X-100, Tween-20, other surfactants, DTT, a protein protective agent, other protective agents, KCl, trehalose, glycerol and deionized water. The nucleic acid rapid release agent can be used for rapid release of DNA and / or RNA in an animal sample, and the obtained release product can be directly or simply treated and then used for nucleic acid amplification detection. The nucleic acid rapid release agent can be operated at normal temperature without relying on instruments, and has the advantages of rapid nucleic acid release, small amplification inhibition, good compatibility and wide sample adaptation range. Meanwhile, the nucleic acid release performance of the nucleic acid rapid release agent is superior to that of a commercial nucleic acid release reagent, and the nucleic acid rapid release agent is suitable for rapid sample pretreatment in nucleic acid molecular detection of animal diseases.

This invention belongs to the field of microbial fermentation technology, specifically relating to a method for preparing microbial inoculants through mixed fermentation of Bacillus subtilis and Bacillus cereus. The method includes: separately activating Bacillus subtilis and Bacillus cereus to obtain a seed culture; adding a basic fermentation medium to a fermenter, first inoculating Bacillus subtilis, and then inoculating Bacillus cereus after it enters the mid-logarithmic growth phase, achieving sequential mixed inoculation; employing segmented temperature control, two-stage pH control, and dynamic sugar feeding during fermentation, while simultaneously adding malic acid in a coupled manner between 20 and 35 hours to alleviate oxidative stress; collecting the cells after fermentation, adding a protective agent, and drying to obtain the inoculant. This invention, through the synergistic effect of sequential inoculation and malic acid coupled feeding, achieves an effective viable cell count of up to 1.0 × 10⁻⁶. 13 With a CFU / mL or higher, the spore formation rate is ≥89%, which is significantly better than the existing mixed-strain fermentation process.

The present application relates to the technical field of microbial feed additive, and discloses a feed lactic acid bacteria additive and a preparation method thereof.The preparation method comprises the following steps: taking Lactobacillus plantarum and / or Enterococcus faecalis as a bacterial strain to activate and expand culture, taking agricultural and sideline product waste as a substrate to obtain mature fermentation liquor through deep liquid fermentation; mixing the mature fermentation liquor with a composite protective agent containing double essential oil, chitosan, theabrownin and porous silicon dioxide to obtain a semi-dry granule core through granulation and low-temperature pre-drying; and coating the semi-dry granule core with a κ-carrageenan / konjac glucomannan / plant sterol ester composite gel, and drying and packaging to obtain a finished product.The double essential oil, chitosan and theabrownin jointly form a full-spectrum active antibacterial system; the elastic composite gel outer layer realizes dual protection of liquid water and water vapor; the porous silicon dioxide actively maintains low water activity of the granule to prevent the lactic acid bacteria from recovering in advance; and the product has high viable count, strong heat and acid resistance, and excellent storage stability.

This invention belongs to the field of biomedical materials and skin / hair repair technology, specifically a recombinant collagen freeze-dried composition targeting hair follicle functional sites, its preparation method, and its application. The composition includes recombinant human collagen, selected from recombinant type III collagen containing a Wnt-binding domain, recombinant type XVII collagen containing an NC16A domain, or a combination thereof; a freeze-drying protectant composed of trehalose and mannitol in a mass ratio of 1:2 to 2:1; and a buffer system, a phosphate buffer with a pH of 6.5-7.5 and a concentration of 5-20 mM. This invention provides precise hair follicle targeting and efficacy far exceeding that of ordinary collagen. It is effective for androgenetic alopecia, telogen effluvium, and diffuse thinning hair loss. It can be used for individuals with scalp micro-damage and sensitive scalps, simultaneously improving the scalp microenvironment and preventing hair loss from the root. Furthermore, its long-term stable effects have been verified over multiple time points.

Water disintegrable, foam producing articles comprise a soluble fibrous structure encasing a particulate effervescent cleaning composition comprising an effervescent agent, a particulate foaming surfactant, an effervescent activator, optionally an effervescent protection agent, and optionally an active agent. Methods of using the articles to clean surfaces are also provided.

This invention also provides a creatine kinase isoenzyme MM detection kit, comprising reagent 1 and reagent 2. Reagent 1 comprises the following components: buffer solution 20-50 mmol / L; creatine kinase isoenzyme B subunit antibody 10-100 mmol / L; creatine kinase MM isoenzyme coated modified magnetic microspheres 0.2-2 g / L; blocking agent 0.5-2 g / L; protective agent 0.5-10 g / L; complex 1-50 mmol / L; stabilizer 1-10 g / L; anti-crystallization component 2-5‰; and preservative 0.05-0.1 g / L. The beneficial effects of this invention are: the kit lowers the limit of detection for creatine kinase isoenzyme MM in serum, enhances specificity, improves repeatability, broadens the linear range, and enhances the hook effect.

The application discloses a ganoderma lucidum hydrophobin Glhyd694 gene, an encoded protein and application thereof, and belongs to the technical field of genetic engineering. The nucleotide sequence of the ganoderma lucidum hydrophobin Glhyd694 gene is shown in SEQ ID NO. 1, and the amino acid sequence of the encoded protein is shown in SEQ ID NO. 2. The ganoderma lucidum hydrophobin has self-assembly characteristics, can perform hydrophobic modification on a material surface, and has excellent emulsification activity and emulsion stability. In the ganoderma lucidum, the protein also participates in tolerance regulation of mycelium to various environmental stresses such as cell wall stress and oxidation stress. The application provides a new gene resource for development of natural surface modified materials and biological protective agents.

The invention belongs to the technical field of biological medicine, and particularly relates to a clostridium baumannii preparation and application thereof in pet disease treatment. The clostridium baumannii is prepared into a freeze-dried microbial inoculum, the freeze-dried microbial inoculum is matched with prebiotics, and the prebiotics can provide nutritional support and enhance the biological activity of the clostridium baumannii; meanwhile, the in-vivo microecological balance can be adjusted, in addition, the protein protective agent, the saccharide protective agent and other components added into the freeze-dried microbial agent can effectively maintain the activity of the clostridium baumannii, the shelf life of the preparation is prolonged, and the stability and reliability of the clinical application effect are guaranteed. According to the invention, a dosage form with an excellent effect is obtained by screening the components with different proportions, and the dosage form shows a more excellent effect in the aspect of treating the dog and cat osteoarthritis. The clostridium baumannii preparation can effectively inhibit inflammatory response caused by arthritis and relieve inflammatory injury, and the raw materials adopted by the clostridium baumannii preparation are natural, high in safety, free of obvious toxic and side effects and suitable for different individual pets.

To provide a method for treating the target cancer. [Solution] A method for treating a target cancer, comprising administering a bispecific antibody having binding specificity to EGFR and HER3 and a therapeutic agent to the target, wherein the therapeutic agent includes a tyrosine kinase inhibitor (TKI), an alkylating agent, an antimetabolite, a microtubule inhibitor, an anti-cancer antibiotic, a topoisomerase inhibitor, a chemoprotective agent, or a combination thereof.

This invention discloses a *Lactobacillus plantarum* bacterial powder, its preparation method, and its application. The wall material of the bacterial powder includes resistant starch, whey protein, and a heat protectant. The bacterial cells in the powder are *Lactobacillus plantarum* TH103. The mass percentages of resistant starch, whey protein, and the heat protectant are 7-23%, with the total mass percentage of resistant starch and whey protein being 3-15% and the mass percentage of the heat protectant being 4-8%. The viable count of the bacterial powder is (3.5-9.0) × 10⁻⁶. 9 The CFU / g and moisture content are 5-7%. This invention uses resistant starch and whey protein as protective carriers, supplemented with heat-resistant protective agents, to achieve the preparation of *Lactobacillus plantarum* powder by spray drying. The obtained *Lactobacillus plantarum* powder has a viable count and moisture content that meet the relevant requirements for probiotic foods, and has good powder properties. It can be directly brewed in powder form or applied to fermented foods such as yogurt.

1. Name of the designed product: tray (three empty). 2. Use of the designed product: used for containing fluorine protectant. 3. Design points of the designed product: combination of shape and pattern. 4. Picture or photo best indicating the design points: perspective view 1.

This invention discloses a citrus peel biomatrix material, its preparation method, and its application, belonging to the field of biomaterials technology. The preparation method of the citrus peel biomatrix material includes the following steps: soaking citrus peel in an acidic solution, then removing it and air-drying it to obtain a pretreated citrus peel material; soaking the pretreated citrus peel in a nutrient solution to obtain an intermediate citrus peel material; coating the surface of the intermediate citrus peel material with photosynthetic bacteria powder, then culturing it under light to obtain a composite citrus peel material; and coating the surface of the composite citrus peel material with a protective agent to obtain the citrus peel biomatrix material. The citrus peel biomatrix material of this invention can better remove organic pollutants from wastewater. Simultaneously, this citrus peel biomatrix material degrades slowly. Therefore, this citrus peel biomatrix material has good application prospects in wastewater treatment.