1727 results about "Protective Agents" patented technology

The invention belongs to the technical field of biology, and discloses a stable protective agent, a cell antigen dry sheet and a preparation method and application of the dry sheet, the stable protective agent comprises glycerin with the volume fraction being 0.4%, gelatin with the mass volume fraction being 0.1%, EDTA with the mass volume fraction being 0.1 mM, trehalose with the mass volume fraction being 0.03% and resveratrol with the mass volume fraction being 100 [mu] M. The stable protective agent is adopted for preparing the cell antigen dry sheet, and the cell antigen dry sheet is applied to CBA detection. The stable protective agent has good permeability, can penetrate through cell membranes to enter cells, provides a protection effect inside and outside the cells, has moisture retention and oxidation resistance, can prevent cell deformation and cell fixing layer shedding, and can well maintain the stability and reliability of antigen proteins, and the prepared cell antigen dry sheet can be stored for a long time, and has a good application prospect. The antigenicity of a membrane signal index is not damaged. When a positive sample is detected, obvious green fluorescence cells can be seen, red fluorescence quenching is avoided, and co-localization of red fluorescence and green fluorescence can be observed conveniently.

The invention relates to the technical field of biological medicine, in particular to eye drops containing recombinant protein as well as a preparation method and application of the eye drops. Comprising the following components: 0.01%-1% of recombinant human TFF3 protein, 0.1%-1% of an osmotic pressure regulator, 0.08%-2% of a pH regulator, 0.05%-0.15% of a thickening agent, 0.01%-0.1% of a surfactant, 0.01%-0.1% of a protective agent and the balance of water for injection, the traditional Chinese medicine composition can be used for treating the xerophthalmia, can promote wound healing and cell migration, has the effects of inhibiting inflammation and cell apoptosis, and can act on a plurality of target spots at the same time, so that the tear film stability is improved, ocular surface inflammation is inhibited, the vicious circle of the xerophthalmia is broken, and the xerophthalmia is treated more comprehensively and effectively; the problems that in the prior art, xerophthalmia medicine targets are single, and xerophthalmia cannot be comprehensively and effectively treated are solved.

The invention is applicable to the technical field of biological detection, provides a fluorescence immunochromatography detection method based on monatomic nano-enzyme enhancement, and particularly relates to two application forms: in the first form, a detection signal enhanced fluorescence immunochromatography detection card and a sample diluent containing a monatomic nano-enzyme are involved; in the second form, the invention relates to a fluorescence immunochromatography detection card with enhanced detection signals, and a sample pad of the detection card is obtained by soaking a sample pad treatment solution containing a monatomic nano-enzyme solution. The two application forms both relate to a monatomic nano-enzyme solution, and the solution is composed of monatomic nano-enzyme, bio-enzyme composite microspheres, a protective agent, a surfactant, a thickening agent and a preservative. The monatomic nano enzyme is composed of an atomic-scale dispersed metal active center and a carrier material. When the method is used for detecting myocardial and inflammatory markers, the limit of quantitation can reach the pg / mL level, the detection linear range can span three orders of magnitude, and the clinical examination use requirements can be met. The kit can be applied to rapid quantitative detection of myocardial and inflammatory markers, has the advantages of high sensitivity, rapidness, convenience and high efficiency, and provides an auxiliary diagnosis basis for early screening of myocardial infarction, heart failure and infection related diseases.

The invention discloses a filling and repairing agent for promoting angiogenesis and a preparation method thereof, the filling and repairing agent is composed of a vascular endothelial cell supernatant, acellular matrix particles, a suspending aid and a protective agent, the vascular endothelial cell supernatant is prepared by inducing vascular endothelial cells into a tube under a low-oxygen three-dimensional condition, collecting a cell culture solution, centrifuging and concentrating; the acellular matrix particles are obtained by acellular tissue matrix, shearing, freeze-drying, liquid nitrogen grinding and collecting; according to the filling repairing agent for promoting angiogenesis, the vascular endothelial cell supernate and the acellular matrix particles are combined, the filling repairing agent contains high-concentration angiogenesis-related factors and a stable three-dimensional bionic scaffold structure, function complementation is achieved through the synergistic effect of the bioactive factors and the bionic scaffold, and the filling repairing agent for promoting angiogenesis is obtained. Therefore, the filling repair agent which can promote angiogenesis, construct a physical support framework and promote tissue repair and regeneration capacity is innovatively obtained.

The invention belongs to the field of biology, and discloses a CRISPR / Cas12a freeze-dried microsphere, which is characterized in that the CRISPR / Cas12a freeze-dried microsphere is obtained by freeze-drying the following raw materials: enzyme-free water, 10 * HOLMES Buffer for Cas12a, an RNA enzyme inhibitor, CRISPR / Cas12a protein, crRNA, an upstream primer F, a downstream primer R, a fluorescent probe Reporter and a freeze-drying protective additive; the CRISPR / Cas12a protein is an LbaCas12a protein, and the CRISPR / Cas12a protein is a Cas12a protein; the freeze-drying protective agent is prepared from mannitol, trehalose, polyethylene glycol, glucan and BSA (Bovine Serum Albumin). By further optimizing a freeze-drying protective agent, freeze-drying microballing of a CRISPR / Cas12a system is achieved, the freeze-drying microballing can be matched with RPA constant-temperature amplification double microballing, and detection is conducted through a one-step method. Meanwhile, the invention further discloses a three-microsphere detection system, application and a kit.

The invention relates to the technical field of metal material hot working, and discloses a hot-rolled coiled plate production process which comprises the following steps: preparing a reaction type protective agent, the high-temperature-resistant coating is prepared from the following raw materials in parts by weight: 20 to 40 parts of tetraethoxysilane, 5 to 15 parts of triethyl phosphate, 1 to 5 parts of triethyl borate, 0.5 to 2.0 parts of cerous nitrate (III) hexahydrate, 0.1 to 1.0 part of lithium acetate and 0.01 to 0.05 part of vanadyl acetylacetonate (IV). Coating the surface of a hot-rolled plate blank with the protective agent; the coated plate blank is heated in a heating furnace, so that the protective agent forms a molten-state protective layer in situ; carrying out hot rolling on the plate blank with the molten protective layer; and cooling and coiling the hot-rolled steel strip. In the whole heating and hot rolling process, the molten protective layer isolates the contact between the steel and the oxidizing atmosphere, so that the generation of oxide scales is fundamentally inhibited, the production process is simplified, and the metal yield is increased.

The invention discloses a bacillus coagulans feed additive and a preparation method thereof, and relates to the technical field of feed additives, the bacillus coagulans feed additive comprises bacillus coagulans spores, the content of the spores is 1.0 * 10 < 8 >-1.0 * 10 < 11 > CFU / g; the protective agent system comprises the following components: maltodextrin or lactose, vegetable protein, polyhydric alcohol and an antioxidant; the invention discloses a microcapsule coating structure. A microcapsule is a two-layer composite coating of an inner layer alginate matrix and an outer layer polycation or polymer coating. According to the invention, bacillus coagulans spores which can still keep high survival rate under short-time high-temperature and low-pH conditions are obtained through screening and fermentation strategies of strains with high sporulation and heat and acid tolerance, the problem that common strains are easy to inactivate in granulation, extrusion and gastric acid environments is solved, the problem of product failure caused by insufficient tolerance of the strains is reduced, and the product quality is improved. And the original biological activity basis is improved, so that the protection effect of the subsequent formula and the coating can be played at a higher starting point, and the zoology effect of the final preparation is improved.

The invention discloses a preparation method of bacterial powder of clostridium butyricum, and belongs to the technical field of bacterial powder preparation. The preparation method of the bacterial powder provided by the invention comprises the following steps: adding a regulator for regulating culture, heating, and adding a protective agent for freeze drying. Experimental results show that the preparation method of the bacterial powder provided by the invention has high viable bacterium yield, and the loss of clostridium butyricum in the preparation process of the bacterial powder is reduced; the bacterial powder obtained according to the bacterial powder preparation method still has the retention rate of 90% or above after being stored for a long time for 180 days at the temperature of 35 DEG C, which indicates that the survival ability of clostridium butyricum is remarkably improved. The preparation method of the bacterial powder is simple in process, easy to operate and environmentally friendly, and the cost cannot be remarkably increased. The clostridium butyricum microecological product provides a new strategy for preparation and activity maintenance of clostridium butyricum microecological products, can be applied to the fields of animal husbandry industry and medical care, and has a wide application prospect.

The invention provides an extraction method of hirudin, an oral preparation containing the hirudin and a preparation method of the oral preparation, and belongs to the technical field of leech processing and traditional Chinese medicine. The hirudin extraction method comprises the following steps: S1, soaking fresh leeches in a freeze-drying protective agent, taking out the leeches, wiping the surfaces of the leeches, pre-freezing the leeches at the temperature of 40 DEG C below zero to 50 DEG C below zero for 1-2 hours, and freeze-drying the leeches at the temperature of 20 DEG C below zero to 30 DEG C below zero for 4-6 hours to obtain freeze-dried leeches; s2, crushing the freeze-dried leeches, adding the crushed leeches into water, adding enzyme and lecithin, performing enzymolysis for 4-6 hours under the conditions that the temperature is 35-40 DEG C and the pH value is 7.5-8.5, and cooling to room temperature to obtain an enzymolysis product; s3, sterilization, ultrafiltration and drying are conducted, and hirudin is obtained. The hirudin extracted by the method is high in activity and high in recovery rate.

The present invention relates to the technical field of microbial deodorants, and discloses a microbial composite deodorant and a preparation method thereof. The microbial composite deodorant comprises a bacterial powder mixture, which is embedded in a load material, and the load material is externally wrapped with a protective agent. The bacterial powder mixture comprises lactic acid Pediococcus, Bacillus megaterium, Alcaligenes faecalis, Bacillus subtilis, and Azotobacter chrysogenum, wherein the mass ratio of lactic acid Pediococcus, Bacillus megaterium, Alcaligenes faecalis, Bacillus subtilis, and Azotobacter chrysogenum is 1:1-3:1-2:0.5-1:1-2. The present invention adopts the above-mentioned microbial composite deodorant and a preparation method thereof. The prepared microbial composite deodorant can significantly remove malodorous gases such as NH3 and H2S in biological feces, has a long action time, and has a significant effect. At the same time, the raw materials of the microbial composite deodorant are green and safe, reducing health problems caused by accidental ingestion by livestock.

The invention discloses high-activity plant lactobacillus freeze-dried powder and a preparation method and application thereof.According to the method, viable bacteria of plant lactobacillus fermentation liquor are increased to 1.66 * 10 < 10 >-1.82 * 10 < 10 > CFU / mL through a high-density fermentation and one-time material supplementing technology, the technology is low in production cost and easy to operate, and the problem that the viable bacteria number of the plant lactobacillus fermentation liquor is low is effectively solved. The freeze-drying protective agent is optimized by adding the plant peptide, the animal peptide and the polyphenol into the basic protective agent, the viable count of the plant lactobacillus freeze-dried powder can be increased to 1.25 * 10 < 12 >-2.55 * 10 < 12 > CFU / g, and the key problem of preparation of high-activity probiotic powder is effectively solved. At the same time, the addition of the active substances enriches the nutrition and efficacy of the freeze-dried powder, and provides a certain experimental basis for the development and application of plant lactobacillus functional food.

Intermolecular interactions and molecular chain mobility are improved when a hydrocarbon-terminal-group-containing compound represented by formula (1) is used as a surface protective agent for a non-fluorine-based material, and a surface treatment agent containing the compound can form a cured coating film having excellent water repellency, slipperiness, ease of soil removal, and abrasion resistance, in particular, steel wool abrasion resistance. (R is a C1-60 monovalent hydrocarbon group, Z is a divalent connecting functional group containing at least one selected from O, N, S, and Si, Y is a C1-30 divalent hydrocarbon group, A is a monovalent reactive group, and k is 1-3.)

The invention relates to the technical field of antistatic agents, and particularly discloses an oil product antistatic agent and a preparation method thereof. The modified polysulfone is obtained by mixing and treating the following raw materials in parts by mass: 35-40 parts of xylene, 20-25 parts of composite polyamine, 15-20 parts of polysulfone, 5-8 parts of oligomeric dopamine and 1-2 parts of an activating agent, the preparation method comprises the following steps: taking composite polyamine, mixing with oligomeric dopamine, dispersing with xylene, then adding an activator, stirring, then adding polysulfone, and continuously treating, thereby obtaining the product. The preparation method of the composite polyamine comprises the following steps: taking epichlorohydrin, dispersing, adding a mixed amine source, heating, adding sodium carbonate, continuously stirring, cooling, adding a deprotection agent, filtering after treatment, taking a liquid part, drying by distillation, washing, drying again, and grinding to obtain the composite polyamine. The oil product antistatic agent prepared by the invention has relatively strong antistatic capability and relatively long duration time, and can form a firmer double-electric-layer structure.

The invention relates to the technical field of separation and purification, in particular to a purification method of eugenol, which comprises the following steps: (a) dissolving crude eugenol in an ethanol aqueous solution to prepare a solution with eugenol mass concentration of 20-30%; (b) adding a composite protective agent into the solution, uniformly stirring and standing to obtain a mixed solution, the composite protective agent comprising modified chitosan, sodium ascorbate and nano-silica; (c) carrying out reduced pressure evaporation on the mixed solution, and recovering the solvent to obtain a concentrated solution; (d) adding the concentrated solution into a glycine aqueous solution with the mass concentration of 1-2%, uniformly stirring and standing; and (e) carrying out segmented temperature control rectification on the concentrated solution treated in the step (d), and collecting a target fraction to obtain the high-purity eugenol. By introducing a novel protective agent system and a staged temperature control rectification technology, the occurrence rate of thermal decomposition and oxidation reaction is remarkably reduced, and the product purity and the production efficiency are improved.

The invention discloses a biological challenge device with combined adjustable resistance for evaluating the effect of a sterilization process, which comprises a shell, the shell comprises a plastic pipe and an inner pipe cap detachably connected with the inner wall of the plastic pipe, the inner pipe cap is provided with a breathable window and covered by a breathable film, and a bacterial contamination carrier for bearing indicator biological spores is attached to the inner bottom of the plastic pipe. An openable container containing a liquid culture medium is arranged in the plastic pipe cavity; the outer side of the inner pipe cap is sleeved with an outer pipe cap detachably connected with the outer wall of the plastic pipe, and the outer pipe cap is provided with a flow blocking structure used for adjusting the speed of the sterilizing agent entering the area of the inner pipe cap; the indicator biological spores are pretreated by a stable protective agent containing one or more of polyethylene glycol, trehalose, sorbitol, polyvinylpyrrolidone and glyceryl stearate. The speed of a sterilizing agent entering the device can be limited, the spores are dry in a suspension added with a stable protective agent, the capability of resisting general environmental changes is very good, and the performance of the device can be maintained for a long time.

The invention relates to the technical field of fertilizers and pesticides for agricultural production, and discloses a liquid pesticide fertilizer for preventing and treating root-knot nematodes in a water-fertilizer integrated system and application thereof, and the liquid pesticide fertilizer is prepared from the following components: a plant source nematicide, a biocontrol inoculant, a macroelement and medium trace element fertilizer, a boron cross-linking agent, a modified polycarboxylic acid dispersing agent, a microbial protective agent and the like. The preparation method comprises the steps of biological phase pretreatment, matrix phase construction, oil phase micro-emulsification, multiphase fusion and dynamic crosslinking. Through trehalose gradient permeation pretreatment, the survival rate of biocontrol bacteria in a high-salt matrix is increased; a boric acid ester bond dynamic cross-linked network is constructed by using a variable pH adjusting process, so that the system is endowed with shear thinning characteristics, and the contradiction between suspension stability and drip irrigation fluidity is solved; and the chelating dispersion effect of the modified polycarboxylic acid dispersing agent is matched, so that the generation of precipitates is effectively inhibited, and stable coexistence and synergistic interaction of the pesticide, the fertilizer and the bacteria are realized.

The invention belongs to the technical field of mixing, and particularly relates to a mixing device and method for flue corrosion protection agent production, the mixing device comprises a mixing barrel and an inner barrel fixedly arranged in the mixing barrel, a cover plate is fixedly arranged on the mixing barrel, a rotating barrel is rotationally arranged on the inner barrel in a sleeving mode, and tooth protrusions are arranged on the rotating barrel; magnetic strips with alternating polarities are fixedly arranged in the rotating barrel in an array manner; wherein a spline sleeve shaft of the telescopic rotating mechanism is combined with a reciprocating groove of a sleeve and a convex rod of an embedded assembly, so that the telescopic mixing paddle can be driven to do composite motion of rotating and lifting, and meanwhile, a movable plate of the telescopic mixing paddle does horizontal reciprocating sliding under the action of attraction force and repulsive force of a magnetic strip and a magnetic block and an elastic telescopic rod, so that three-dimensional composite stirring is formed; the rubber scraping plate moves along with the moving plate, raw materials attached to the inner wall of the inner barrel can be intermittently scraped off, caking of the raw materials is avoided, and when the mounting base synchronously moves along with the moving plate, the inclined sawteeth on the mounting base can effectively cut powder balls in the raw materials.

The invention relates to the field of freeze-drying protective agents, and particularly discloses a vacuum freeze-drying protective agent for lactobacillus plantarum LS-41. The vacuum freeze drying protective agent for the lactobacillus plantarum LS-41 comprises the following components: 15 parts of dried skim milk, 3 parts of cane sugar, 1 part of PBS dry powder, 6-10 parts of trehalose, 0.2-0.4 part of ascorbic acid, 0.06-0.10 part of sodium glutamate, 8-12 parts of sorbitol and 100 parts of ultrapure water. Through the synergistic effect of the trehalose, the ascorbic acid, the sodium glutamate, the sorbitol, the skimmed milk powder and the cane sugar, and improvement and reasonable matching of the category and the concentration of the protective agents, the damage of the lactobacillus plantarum LS-41 in the vacuum freeze-drying process can be reduced, and the freeze-drying survival rate of the lactobacillus plantarum LS-41 is increased.

The invention discloses a cryoprotectant which comprises glycerol, skimmed milk powder, mannitol, trehalose, vitamin C, fructo-oligosaccharide, inulin and sterile water. By optimizing the components of the cryoprotectant, the activity of the coprophilous fungi can be effectively protected, the survival rate of the intestinal coprophilous fungi in the freezing process is remarkably increased, all the used components are food-grade edible components and have a wide application range and economic effects, the invention further discloses a coprophilous fungi freezing preservation method adopting the cryoprotectant, and the cryoprotectant has good application prospects. The method is simple, easy to operate and suitable for large-scale production and application.

The invention provides a cyclic peptide used for treating, improving or preventing nervous system injury of mammals or diseases or pains caused by the injury, neurodegenerative diseases, anxiety or epilepsy or used as a neuronal protective agent, a conjugate containing the cyclic peptide, a pharmaceutical composition containing the cyclic peptide or the conjugate and application of the cyclic peptide and the conjugate.

The invention discloses a QS-21 composite adjuvant and a preparation method thereof, and belongs to the technical field of biological medicines. According to the QS-21 composite adjuvant, a polyanion material and QS-21 are compounded to form nanoparticles, and the nanoparticles are organically combined with a freeze-drying protective additive, so that the prepared QS-21 composite adjuvant is relatively high in retention rate, the problems of hydrolysis and oxidation are solved, the requirement of a phospholipid carrier is avoided, and a vaccine co-preparation process is simplified; the subsequently added freeze-drying protective agent retains the immune enhancement activity of the QS-21 and reduces the exposure of the QS-21 to a degradation environment; the corresponding cytotoxicity meets the standard, and the hemolysis rate is low; therefore, the method has remarkable clinical application value and industrialization potential.

The invention discloses bee venom-containing skin essence with small irritation and a preparation method of the bee venom-containing skin essence. The skin essence is prepared from PLGA-PEG / liposome nanoparticles, a temperature-sensitive gel matrix, a protective agent, an antioxidant, a stabilizer and ultrapure water, the preparation method comprises the following steps: preparing the PLGA-PEG / liposome nanoparticles, preparing a poloxamer 407 solution, resuspending the nanoparticles, filling and storing. According to the invention, the melittin and the snake venom-like peptide have a synergistic effect on skin wrinkle resistance, so that the skin care effect is improved; pLGA-PEG / liposome nanoparticles are matched with a poloxamer solution to construct a dual sustained-release drug system, so that the irritation of drugs to skin is reduced, the lasting effect of the drug effect is improved, and the obtained skin essence has excellent moisturizing, whitening, anti-wrinkle and antioxidant capacities and is small in irritation.

The invention relates to a method for preparing high-molecular-weight polyethylene furandicarboxylate by a one-step method, and belongs to the technical field of bio-based polyester materials. The method comprises the following steps: weighing raw materials including 2, 5-furandicarboxylic acid, ethylene glycol, an end group regulator, a protective agent, a decarboxylation inhibitor and a catalyst, mixing and adding into a reaction container, introducing nitrogen, maintaining the pressure of the nitrogen in the reaction container to be 50-150 kPa, carrying out esterification reaction, heating and vacuumizing until the pressure is less than or equal to 50 Pa after the esterification reaction is completed, and carrying out polycondensation reaction to obtain the 2, 5-furandicarboxylic acid. And carrying out extrusion and drying so as to obtain the high-molecular-weight polyethylene furandicarboxylate, wherein the high-molecular-weight polyethylene furandicarboxylate is obtained. According to the method, the FDCA conversion rate is larger than or equal to 98%, the number-average molecular weight of the obtained product is larger than or equal to 32 kg / mol, no heavy metal residues exist, and the method is suitable for large-scale production of high-end bio-based packaging materials.

The invention discloses stem cell freeze-dried powder for promoting skin healing and a preparation method of the stem cell freeze-dried powder. The freeze-dried powder comprises an effective component, a freeze-drying protective agent group and a polymer protective agent group, the effective component is umbilical cord mesenchymal stem cell conditioned medium freeze-dried supernatant, the protective agent group is selected from one or more of cane sugar, trehalose, lactose and mannitol, and the polymer protective agent group is selected from one or more of polyvinylpyrrolidone (PVP) and glucan. The preparation method comprises the following steps: collection of a conditioned culture medium, preparation of a freeze-drying protective agent solution, mixing treatment, low-temperature pre-freezing and freeze-drying, collection and packaging of freeze-dried powder and the like. The method can effectively retain stem cell-derived active factors and enhance the stability and redissolvability of the freeze-dried powder, and is suitable for skin wound repair, regenerative medicine and other scenes.

The invention discloses a freeze-drying protective agent composition, a freeze-drying protective agent, application of the freeze-drying protective agent, a probiotic freeze-dried product and a preparation method of the probiotic freeze-dried product. The freeze-drying protective agent composition comprises the following components in parts by weight: 0.5-4 parts of tremella polysaccharide; the relative molecular weight of the tremella polysaccharide is 1 KDa to 10 KDa; 0.5 to 4 parts of disaccharide; and 0.5-2 parts of sugar alcohol. The freeze-drying protective agent composition and water are uniformly mixed and then sterilized to obtain the freeze-drying protective agent, and the freeze-drying protective agent is used for preparing probiotic freeze-dried products and can effectively ensure the activity and stability of probiotics.

The invention discloses a gold nanocluster electrogenerated chemiluminescence probe which is a multifunctional gold nanocluster obtained by taking a 2-sulfydryl-1, 3, 4-thiadiazole ligand as a reducing agent or a protective agent. Comprising a solid-phase probe with a gold cluster modified on an electrode; or the gold cluster is independent of the electrode and is a liquid-phase probe dissolved in the electrolyte during use. The obtained electrogenerated chemiluminescence gold nanocluster probe can generate an electrogenerated chemiluminescence signal under the condition of no co-reactant, and is relatively low in luminescence potential, high in quantum yield and good in biocompatibility.

Methods for treating a neuropsychiatric disorder by administering an iboga alkaloid and a cardioprotective agent in conjunction with analysis of brain image data is described. Also described are methods to improve brain health and to slow or reverse brain aging by disorder by administering an iboga alkaloid and a cardioprotective agent, where analysis of brain image data is used to monitor and / or evaluate treatment effectiveness.

In some embodiments provided herein is a method of treating a coagulopathy in a subject, the method including administering to the subject in need thereof an effective amount of a composition including platelets or platelet derivatives and an incubating agent including one or more salts, a buffer, optionally a cryoprotectant, and optionally an organic solvent, wherein the subject has been treated or is being treated with an antiplatelet agent.

A Solid Phase Peptide Synthesis (SPPS) device and method of using the same for manufacturing peptides is taught herein. The system comprises at least two reactors, each reactor including a quantity of SPPS resin. The reactors are positioned in series. A de-protecting agent is added to the first reactor and then transferred to the second and third reactors, in series, thereby operating to de-protect the protected N-group. Wash solvent is added to the first reactor and then transferred to the second and this operation repeated several times. Likewise, an amino acid activated ester solution is added, in series, to the first, second and third reactors, thereby operating to couple the amino acid to the de-protected N-group. Wash solvent is added to the first reactor and then transferred to the second and this operation repeated several times prior to the next cycle. The use of the reactors in series reduces the overall solvent required. Online LCMS is also used to monitor progress and identity of reactions happening within the solid phase resin particles.

The invention relates to the technical field of organic high-molecular compound synthesis, in particular to a modified ABS material diffusion plate and a preparation method thereof.The preparation method comprises the steps that dried ABS resin and acrylonitrile-butadiene-styrene copolymer are mixed to prepare a modified ABS base material; mixing organic silicon microspheres and nano barium sulfate to obtain a modified light diffusion agent; judging that the modified light diffusant is unqualified by comparing the refractive index absolute difference between the modified light diffusant and the modified ABS base material, and performing modification compensation; a titanate coupling agent is determined to be adopted for modification according to the content of inorganic particles of the modified light diffusion agent; mixing the modified light diffusant with an antioxidant system, an ultraviolet protective agent and a processing aid to obtain a functional additive; and mixing with a modified ABS (Acrylonitrile Butadiene Styrene) base material, and extruding by using a twin-screw extruder to Through multi-parameter feedback and accurate compensation control, the problems that the compatibility of the modified light diffusion agent and the base material is poor and the stability of the machining process is insufficient are effectively solved.