13 results about "Gastrointestinal digestion" patented technology

The application discloses a DPP-IV inhibitory activity peptide as well as a preparation method and application thereof. The active peptide is a pentapeptide as shown in a general formula X-P-A-P-Y, wherein X is leucine L or valine V, and the active peptide is specifically LPAPY or VPAPY, and further includes pharmaceutically acceptable salts, N-terminal acetylation modifiers and C-terminal amidation modifiers of the active peptide. The active peptide meets the requirements of a DPP-IV inhibitory activity retention rate of greater than or equal to 60% under in-vitro simulation of gastrointestinal digestion conditions, a DPP-IV inhibitory activity IC 50 value of less than or equal to 160 micromoles, and has high inhibitory activity and gastrointestinal digestion stability. The application uses silkworm chrysalis protein as raw material, and extracts the active peptide through sequential combined enzymolysis of momordica grosvenori proteinase and papain, or prepares the active peptide with high purity through a solid-phase synthesis method.

This invention belongs to the fields of food biotechnology and food processing, and discloses a method, product, and application for regulating the allergenicity of soybean protein based on heat-induced proanthocyanidin covalent modification. This invention aims to solve the problem of unstable regulation of soybean protein allergenicity during heat processing, and provides a stable and differentiated method for reducing allergenicity. The core of the method lies in: under heat treatment conditions, proanthocyanidins simultaneously covalently modify soybean 7S and 11S globulins, achieving differentiated regulation of their allergenicity. Products using this method exhibit the following specific pattern of allergenicity: 7S globulin products are significantly lower than heat-treated products; 11S globulin products are lower than heat-treated products and natural proteins. This low-allergenicity pattern remains stable after in vitro simulation of adult gastrointestinal digestion. This invention provides a targeted, mild, and efficient allergen regulation strategy, and the resulting products can be used to develop low-allergenic foods, special medical purpose formula foods, and dietary supplements.

This invention discloses a method for preparing and applying an antioxidant and intestinal-delivered astaxanthin-loaded microalgae. First, hyaluronic acid-modified astaxanthin liposomes with controlled intestinal release are synthesized. Then, the carboxyl groups on the astaxanthin liposomes are activated using EDC / NHS reagents to couple with the amino groups on Chlorella. After oral administration, this drug-loaded microalgae can effectively remain in the intestinal tract and gradually degrade, slowly releasing astaxanthin. This significantly increases the astaxanthin concentration in the intestine, maximizing astaxanthin absorption and utilization while minimizing astaxanthin loss through the digestive tract. The astaxanthin released by this drug-loaded microalgae after simulated gastrointestinal digestion exhibits strong free radical scavenging and antioxidant capabilities in its polar ketone structure, showing broad application prospects in health and nutrition aspects such as anti-inflammation, photoprotection, immune enhancement, and anti-aging.

The application provides a high-temperature-resistant and gastric acid-resistant probiotic preparation and a preparation method thereof, and the preparation comprises a probiotic core layer and a shell layer covering the same, and the shell layer comprises succinyl-modified food leaf protein assisted by ultrasonic pretreatment, peach gum polysaccharide and soybean lecithin. The application constructs a single-layer protection system with excellent responsiveness by performing specific functional modification on the food leaf protein and synergizing the food leaf protein with the peach gum polysaccharide and the soybean lecithin. The preparation can significantly improve the survival rate of probiotics during processing, storage and simulated gastrointestinal digestion, and especially realizes excellent gastric acid resistance and intestinal targeting release performance, and the process is simple and suitable for embedding and protecting various probiotics.

This invention provides a traditional Chinese medicine composition for promoting gastrointestinal digestion, its preparation method, and its application, belonging to the field of traditional Chinese medicine technology. The invention provides a traditional Chinese medicine composition for promoting gastrointestinal digestion, comprising the following raw materials in parts by weight: 5-20 parts of charred hawthorn, 5-25 parts of charred Shenqu (medicated leaven), 5-20 parts of charred malt, and 5-20 parts of Poria cocos. The traditional Chinese medicine composition provided by this invention has good clinical efficacy in treating indigestion in infants and young children, indigestion in adults, and slow recovery of spleen and stomach function after surgery. Furthermore, the formulation is scientific, the compatibility is reasonable, the medicinal resources are abundant, the preparation process is simple, it is easy to carry, convenient to use, inexpensive, highly effective, has no side effects, and is highly safe. This represents a major innovation in medicines for treating indigestion in infants and young children, indigestion in adults, and slow recovery of spleen and stomach function after surgery.

The present application relates to the white lentil polypeptide with antioxidant activity and its preparation method and application, belong to the technical field of bioactive peptide. The amino acid sequence of the white lentil polypeptide with antioxidant activity is shown as SEQ ID NO.1, its preparation method is to extract the extract with white lentil as raw material, then the pepsin is used for enzymolysis, separation and purification, freeze-drying, and the white lentil polypeptide with antioxidant activity is obtained. The white lentil polypeptide has excellent free radical scavenging capacity, and can still maintain high activity under the harsh heat, acid and alkali and simulated gastrointestinal digestion environment, which provides key support for the practical application feasibility of the white lentil polypeptide as oral or external active ingredient.

The application discloses a kind of development and application of marine protein source alcoholism-preventing liver-protecting product, belong to biotechnology field.Product preparation includes the following steps: (1) to marine organism homogenate liquid first protease is added to carry out enzymolysis, inactivation after enzymolysis is completed, obtain primary enzymolysis liquid;Second protease is added to primary enzymolysis liquid to carry out enzymolysis, inactivation after enzymolysis is completed, obtain secondary enzymolysis liquid.(2) the supernatant of secondary enzymolysis liquid is obtained by centrifugation.(3) the supernatant is concentrated, freeze-drying to obtain target powder.The application uses 8 kinds of marine organisms as raw materials, adopts 7 kinds of protease, and obtains 336 samples by double-enzyme stepwise enzymolysis;Based on the stability of gastrointestinal digestion, amino acid composition and molecular weight distribution, the raw material is determined as sea cucumber, and the alcoholism-preventing liver-protecting product with high stability, high hydrophobic amino acid composition and low molecular weight is obtained by stepwise hydrolysis using alkaline protease and flavour protease.The application is suitable for food health care field.

The application discloses a kind of rich in wheat oligopeptide compressed dry food digestion performance evaluation method and device, the method comprises: using in vitro dynamic human gastrointestinal digestion system, not containing wheat oligopeptide compressed dry food and rich in wheat oligopeptide compressed dry food are respectively simulated digestion processing, respectively measured to obtain first measurement index set and second measurement index set;Each type of measurement index set, including gastric emptying curve, protein digestion rate sequence, amino acid composition value sequence, fat bioavailability sequence;The gastric emptying curve includes several discrete points;The first measurement index set and second measurement index set are preprocessed, and obtain preprocessed index information set;The preprocessed index information set is evaluated and processed, and obtains the digestion performance evaluation value of rich in wheat oligopeptide compressed dry food.

A method for screening and preparing antioxidant peptides from mare's milk whey protein and uses thereof. Potential antioxidant polypeptides are screened based on in silico enzymolysis and computer-based prediction, followed by molecular docking with the keap1 protein. The feasibility of obtaining antioxidant peptides is then validated by simulating gastrointestinal digestion using pepsin, trypsin, and chymotrypsin. Subsequently, high-purity antioxidant peptides are chemically synthesized and subjected to in vitro chemical antioxidant activity assays.

This invention discloses a composite nanoparticle loaded with carotenoids. The composite nanoparticle, with zein loaded with carotenoids as the core and a metal-polyphenol network as the shell, significantly enhances the environmental stability (especially photostability and thermal stability) of carotenoids by constructing a core-shell structured nanocarrier. It also improves their dispersibility in aqueous phases and increases their bioavailability during simulated gastrointestinal digestion, ultimately improving their bioavailability and application value. The carotenoid-loaded composite nanoparticle constructed in this invention serves as a general platform for delivering other hydrophobic carotenoids (such as astaxanthin, β-carotene, lutein, etc.), showing broad application prospects.

This invention belongs to the field of functional food processing technology and provides a polypeptide microcapsule and its preparation method. The preparation method is as follows: fish paste is mixed with buffer solution, sonicated, and then trypsin is added for enzymatic hydrolysis for 4-6 hours. After enzyme inactivation, the supernatant is obtained by centrifugation and drying to obtain polypeptides. HPMCP and an ethanol-acetone mixed solvent are mixed and then added to the polypeptide solution. The mixture is stirred for 10-20 minutes to obtain a dispersed phase. Soybean oil and an emulsifier are mixed to obtain a continuous phase. The dispersed phase and the continuous phase are mixed and emulsified. The mixture is stirred at 30-50°C until the solvent evaporates completely. The mixture is filtered, washed, and dried to obtain polypeptide microcapsules. The microcapsules prepared by this invention have an average particle size of 3960 nm and are regularly spherical. Infrared spectroscopy confirms successful encapsulation. In vitro simulated gastrointestinal digestion experiments show that the microcapsules have "stomach-stable intestinal release" characteristics, which can protect tuna polypeptides from degradation in the gastric environment and efficiently release them in the intestinal environment while enhancing antioxidant activity.

This invention discloses a calcium and iron-enhancing donkey-hide gelatin peptide prepared based on a gastrointestinal digestion model, its preparation method, and its applications, belonging to the field of biotechnology. By comparing different animal gelatin bases, this invention selects highly active donkey-hide gelatin as a raw material and simulates in vitro gastrointestinal dynamic digestion to prepare highly absorbable calcium and iron active peptides, effectively improving peptide bioavailability. The preparation method described in this invention can efficiently screen for calcium and iron-enhancing and highly permeable bioactive peptides. The method is simple and easy to implement, enabling high-throughput screening of hundreds of candidate peptides or formulations. The screened peptide products can be specifically applied in functional foods and pharmaceuticals, showing significant market potential and providing new ideas for related research.

The application belongs to the technical field of microorganisms, and particularly relates to application of Bifidobacterium adolescentis in improving muscle mass. The Bifidobacterium adolescentis YBT652 provided in the application is screened from fecal samples of teenagers in Harbin region, and is verified to have good ability of improving muscle mass. The microgel preparation constructed by using the Bifidobacterium adolescentis YBT652 can not only effectively realize embedding of the Bifidobacterium adolescentis, but also can improve the anti-gastrointestinal digestion ability and the adhesion ability in the intestinal tract of the Bifidobacterium adolescentis. Especially, the application induces muscle mass attenuation and simulates the aging process by using d-galactose, reveals the significant effect of the microgel preparation of the Bifidobacterium adolescentis in improving muscle mass attenuation, can provide new ideas and methods for microbial treatment of muscle mass attenuation, and provides technical support and theoretical basis for development of new treatment drugs.