The invention belongs to the technical field of biological
small molecule active peptides, and more specifically relates to a
uric acid reducing active
peptide, a preparation method and an application thereof. The
uric acid reducing activity LQKW has remarkable XOD inhibitory activity, IC50 is 2.70 mg / mL, and the IC50 is superior to that of many known
natural source XOD inhibitory peptides; good
gastrointestinal digestion stability is shown, and the activity
retention rate after in-vitro simulated
gastrointestinal digestion is (61.84 + / -0.82)%. According to the invention, a specific binding mechanism of
uric acid reduction activity and XOD active sites (Ile648, Phe649 and the like) is clarified through molecular docking, and a basis is provided for
rational design based on a structure. According to the invention, the method for efficiently preparing the active
peptide fragment from the
white spirit vinasse is established, and the high-valued utilization of the
wine brewing byproducts is realized. The uric acid reducing activity LQKW provided by the invention can be prepared through
solid-
phase synthesis, the process is mature, the quality is controllable, and a foundation is laid for industrialization. In addition, as the XOD inhibitor of a
natural source, the uric acid reducing activity LQKW has good safety.