99 results about "Gastrointestinal digestion" patented technology

The invention discloses protein-based nanoparticles as well as a preparation method and application thereof. The protein-based nanoparticles have a core-shell structure, the core is zein particles loaded with active drugs, and the shell comprises a trypsin inhibitor. The preparation method of the protein-based nanoparticles comprises the following steps: 1) preparing a zein dispersion liquid loaded with an active drug and a shell material aqueous solution; and 2) slowly adding the zein dispersion liquid loaded with the active drug into the shell material aqueous solution in a stirring state, and then carrying out rotary evaporation and freeze drying. The protein-based nano-particle has an excellent gastrointestinal digestion resisting effect, various hydrophobic active drugs can be effectively delivered to a lower digestive system, the stability and slow release efficiency of the loaded drugs are remarkably enhanced, and the protein-based nano-particle is good in biocompatibility, safe, non-toxic, simple in preparation process and suitable for industrial production. The method is suitable for large-scale industrial production and application.

The invention provides a hypoglycemic peptide which is prepared by taking bovine whey protein as a raw material and is stable in gastrointestinal digestion and absorption. In an in-vitro cell experiment, the peptide fragment can improve the uptake of insulin-resistant HepG2 hepatocytes and synthesize glycogen by using glucose, can promote intestinal endocrine cells NCI-H716 to secrete GLP-1, has a certain synergistic effect, and can further play the effects of promoting GLP-1 secretion and activating a GLP-1 receptor through combined use. An insulin resistance mouse model is constructed by utilizing high-fat diet, and the peptide fragment is adopted for intervention, so that the hypoglycemic peptide is further proved to have obvious effects on losing weight, reducing lipid accumulation and assisting in reducing blood fat, and meanwhile, the hypoglycemic peptide can be used for improving insulin resistance related symptoms. The milk peptide provided by the invention has gastrointestinal digestion stability and hypoglycemic activity, can be used as a functional milk base material for developing food, medicines or health care products with the effect of regulating blood sugar, and is wide in application prospect.

The invention discloses a nutrition improvement nursing method for a gastrointestinal digestion patient, and relates to the field of medical monitoring, and the method comprises the following steps: analyzing to obtain the current absorption amount of to-be-supplemented nutrient elements of the gastrointestinal digestion patient; analyzing to obtain a health influence coefficient of the to-be-supplemented nutrient elements on the gastrointestinal digestion patient; acquiring an absorption promotion coefficient between the to-be-supplemented nutrient elements and other to-be-supplemented nutrient elements; forming a to-be-updated recipe of the current recipe; calculating a health contribution value of the to-be-updated recipe to the gastrointestinal digestion patient; and calculating the coincidence proportion of the to-be-updated recipe relative to the current recipe, and performing recipe change on the current recipe. According to the method, the health influence coefficient is obtained through analysis, the absorption promotion coefficient is obtained, the to-be-updated recipe is formed, the corresponding health influence is obtained according to the deficiency of different to-be-supplemented nutrient elements, and the mutual influence condition existing between the different to-be-supplemented nutrient elements is also considered, so that the prediction result of nutrient absorption is closer to the reality.

The invention discloses a method for improving gastrointestinal tolerance of probiotics and a probiotic liquid core gel ball, the probiotic liquid core gel ball is prepared by adopting a calcium alginate cross-linked reverse spheroidizing method, and various indexes influencing the characteristics of the probiotic liquid core ball after re-culture are optimized, the process is simple in preparation, raw materials are natural and safe, the cost is low, and the method is suitable for industrial production. The tolerance and the survival rate of the probiotics in gastrointestinal digestion can be well improved, and the probiotic liquid core gel ball prepared by the method disclosed by the invention has good mechanical property, swelling rate and storage survival rate; by optimizing various parameters, such as re-culture time, core material liquid viscosity, polysaccharide molecular weight and a storage solution, which influence the characteristics of the probiotic liquid core ball after re-culture, the re-cultured liquid core ball has better mechanical properties, swelling rate and survival rate, and by combining with a gastric acid-cholate resistance reaction result, the survival rate of the liquid core ball is improved. The invention provides the preparation process of the re-culture solution core ball capable of effectively improving the gastrointestinal tolerance of the probiotics whether the core ball is broken or not.

The invention relates to the technical field of food processing, in particular to fish-derived protein gel special for 3D printing dysphagia and having a customized digestion characteristic and a lycopene release function as well as a preparation method and application of the fish-derived protein gel. The lycopene-loaded IN / KGM gel system is constructed by combining a food additive manufacturing technology and a dynamic old people digestion model, and the rheological property and swallowing safety of the gel are optimized by regulating and controlling the ratio of IN to KGM; the customized digestion characteristics of the gel and the adaptability of the gel to the digestion function of the old people are researched by utilizing an in-vitro dynamic digestion model of the old people; the loading and releasing behaviors of lycopene in a gel system are explored, the bioaccessibility of lycopene in the gastrointestinal digestion process of old people is evaluated, the interaction mechanism of IN and KGM in the gel forming and digestion process is disclosed, and a theoretical basis and technical support are provided for developing personalized nutritious food suitable for the old people with dysphagia.

The invention relates to the technical field of small-molecule donkey-hide gelatin peptides, and provides a small-molecule donkey-hide gelatin peptide with iron ion chelating ability and application thereof, the molecular weight of the small-molecule donkey-hide gelatin peptide is smaller than 1000 Da, and the small-molecule donkey-hide gelatin peptide has the iron ion chelating ability. The iron chelation rate of the small-molecule donkey-hide gelatin peptide reaches 95%, and the small-molecule donkey-hide gelatin peptide has good solubility in in-vitro gastrointestinal digestion simulation and can be applied to the fields of iron supplements, nutrition fortified foods and the like.

The invention belongs to the field of food nutrition and functional factors, and particularly relates to a method for preparing ovotransferrin hydrolysate with high immunocompetence based on an adult gastrointestinal simulated digestion system. According to the method, ovotransferrin is treated through the stomach and intestine of a simulated adult, OVT hydrolysate is prepared, and the OVT hydrolysate contains HTEGSTT, TTSY and / or RTAGWVIPMG. The invention finds that the immunomodulatory activity of OVT is enhanced after gastrointestinal digestion and hydrolysis, the OVT is more easily hydrolyzed in simulated adult digestive juice than in infant digestive juice, and a contrast experiment shows that the nutrition intervention of OVT adult and infant simulated digestion products can promote the generation of short-chain fatty acids such as acetic acid, propionic acid and butyric acid; the swelling injury caused by inflammation to small intestine mucous membrane tissues is effectively relieved, so that the intestinal barrier immune function is obviously improved, and the OIA shows higher intestinal immune activity than the OIB.

The invention belongs to the technical field of biological small molecule active peptides, and more specifically relates to a uric acid reducing active peptide, a preparation method and an application thereof. The uric acid reducing activity LQKW has remarkable XOD inhibitory activity, IC50 is 2.70 mg / mL, and the IC50 is superior to that of many known natural source XOD inhibitory peptides; good gastrointestinal digestion stability is shown, and the activity retention rate after in-vitro simulated gastrointestinal digestion is (61.84 + / -0.82)%. According to the invention, a specific binding mechanism of uric acid reduction activity and XOD active sites (Ile648, Phe649 and the like) is clarified through molecular docking, and a basis is provided for rational design based on a structure. According to the invention, the method for efficiently preparing the active peptide fragment from the white spirit vinasse is established, and the high-valued utilization of the wine brewing byproducts is realized. The uric acid reducing activity LQKW provided by the invention can be prepared through solid-phase synthesis, the process is mature, the quality is controllable, and a foundation is laid for industrialization. In addition, as the XOD inhibitor of a natural source, the uric acid reducing activity LQKW has good safety.

The invention discloses a glycinin compound for preventing hyperlipemia as well as a preparation method and application of the glycinin compound, and belongs to the field of biological medicines. The preparation method comprises the following steps: stirring and mixing resveratrol and a soybean 7S globulin solution at 40-60 DEG C for 1.5-2.5 h, so that resveratrol and soybean 7S globulin are fully combined to obtain a glycinin compound; the mass ratio of the soybean 7S globulin to the resveratrol is (250: 50)-(250: 45). Experimental results show that the glycinin compound disclosed by the invention can inhibit the weight increase of a hyperlipemia rat, remarkably reduce the fat index and the liver index, effectively improve the abnormal change of the blood fat factor level and show an obvious lipid-lowering effect. In-vitro simulated digestion experiments show that the glycinin compound shows peptide fragments with different strength from soybean 7S protein after gastrointestinal digestion, and meanwhile, the glycinin compound improves the in-vitro cholate binding capacity.

The invention discloses a preparation method of a red rice bran protein peptide compound with antioxidant and hypoglycemic effects. According to the method, red rice bran protein is extracted through an alkali-solution and acid-isolation method; hydrolyzing the red rice bran protein with alkaline protease to obtain a red rice bran protein hydrolysate; the red rice bran protein hydrolysate prepared by the invention has the functional characteristics of excellent emulsibility, foamability and oil absorbency, and has excellent antioxidant and hypoglycemic functional activities after being subjected to in-vitro simulated gastrointestinal digestion. The red rice bran protein hydrolysate can be used in plant-based food and dairy product substitutes and functional food with a hypoglycemic effect.

The present invention relates to the technical field of whey protein hydrolysis, and particularly to a preparation method and application of buffalo colostrum-derived bioactive peptides. The present invention optimizes the enzymatic hydrolysis process of buffalo colostrum whey protein through single-factor experiments to obtain an enzymatic hydrolysis method of buffalo colostrum whey protein suitable for large-scale production. In addition, the enzymatic hydrolysis process of buffalo colostrum whey protein is optimized by the response surface method. Three factors, namely the pH value, enzymatic hydrolysis time, and enzyme addition amount during enzymatic hydrolysis with neutral protease, are selected as corresponding variables, and the ABTS radical scavenging rate is used as the response value. Multiple fittings are performed on each factor to obtain the optimal enzymatic hydrolysis process conditions for whey protein. The buffalo colostrum-derived bioactive peptides obtained by this enzymatic hydrolysis method have the technical advantages of high ABTS radical scavenging rate and good immune function. At the same time, the stability of the enzymatic hydrolysis product is further studied through gastrointestinal digestion experiments, providing a more comprehensive theoretical reference for the further research of whey protein.

The application discloses a South China Sea Decapterus maruadsi polypeptide with uric acid reducing and antioxidant activities and an application thereof, and relates to the technical field of biology. The polypeptide with high uric acid reducing activity and antioxidant activity is screened through activity prediction and molecular docking, and the amino acid sequence is FYDWWK. The polypeptide can be stably combined with the active center of xanthine oxidase, forms multiple hydrogen bonds and hydrophobic interaction, significantly inhibits the generation of uric acid, and has an in-vitro xanthine oxidase inhibition IC 50 of 3.068±0.2547 mmol / L. Meanwhile, the polypeptide has excellent DPPH free radical scavenging capacity, and an IC 50 of 5.033±0.5476 mmol / L. The polypeptide has good stability, still has high efficient inhibitory activity after simulated gastrointestinal digestion, and has oral development potential. The polypeptide is a natural marine small molecule active peptide, is safe and easy to absorb, and can be applied to the preparation of products related to uric acid reducing and / or antioxidant.

The invention belongs to the technical field of whey protein processing, and particularly relates to a preparation method of a hydrolyzed whey protein digestion product with high immunocompetence and low sensitization. The preparation method comprises the following steps: by taking hydrolyzed whey protein as a raw material, carrying out simulated gastrointestinal digestion treatment under specific conditions to obtain a whey protein digestion product; the product can effectively exert immunoregulation and anti-inflammatory effects, solves the problem of sensitization of a traditional whey protein product, and has a good application prospect.

The invention discloses a preparation method of a whey protein / pullulan / sugar astaxanthin nano-dispersion, WPC / PUL compounds are adopted to construct different carriers to embed AST, the carriers are respectively a nano-dispersion loaded with AST, emulsion gel and microcapsule powder, the three carriers have good embedding efficiency and protection effect on AST, the simulated gastrointestinal digestion stability of AST is remarkably improved, and the stability of AST is improved. And the method has great significance in expanding the preparation of an AST embedding delivery system and the application of AST in industries such as food and the like.

The invention relates to the technical field of food, in particular to rumex hanus and burdock green juice with multiple effects, and the rumex hanus and burdock green juice mainly comprises rumex hanus and burdock. The rumex hanus and the burdock are nutritional supplements based on plants, contain a large amount of protein, amino acid, vitamins, mineral substances and various plant compounds, and can achieve the effects of improving gastrointestinal digestion, resisting oxidation, reducing blood sugar and blood pressure and improving immunity.

The invention relates to the technical field of probiotic embedding, in particular to a probiotic microcapsule as well as a preparation method and application thereof. The probiotic microcapsule comprises probiotics and a composite wall material used for embedding the probiotics, the probiotics are probiotics with negative charges, and the composite wall material is composed of carboxymethyl pachymaran and soybean protein isolate. The probiotic microcapsule provided by the embodiment of the invention has significantly improved survival rate in gastrointestinal tracts and under low-temperature and normal-temperature storage conditions, has high stability and is suitable for freeze drying, and the final viable count exceeds 10 < 6 > CFU / g after gastrointestinal digestion and long-term storage, so that the probiotic microcapsule reaches the standard of probiotic food, and has good application prospects. The method has important research value and market prospect.

The invention relates to a method for preparing colla corii asini oligopeptide by adopting a dynamic bionic digestive system, which comprises the following steps: preparing digestive juice simulation liquid which comprises oral cavity digestive juice, stomach digestive juice and intestinal digestive juice; the donkey-hide gelatin peptide sample simulates oral digestion; starting a dynamic human body bionic digestion system, adding a sample digested by an oral cavity into the system, adding gastrointestinal digestion liquid into the system, and simulating a dynamic gastrointestinal digestion process; collecting gastrointestinal digestion products, carrying out water bath enzyme deactivation, cooling, centrifuging, separating supernate and precipitate, and detecting molecular weight distribution of the supernate; and freeze-drying the supernate into powder to obtain the donkey-hide gelatin oligopeptide. The colla corii asini peptide is treated by adopting a dynamic bionic digestive system, the digestion process of a human body is simulated, and the colla corii asini oligopeptide is obtained, so that blood-enriching active substances in the colla corii asini peptide are better released, human body absorption is facilitated, and the bioavailability and the blood-enriching effect of the colla corii asini peptide are improved.

The invention discloses oligopeptide and application thereof in preparation of a medicine for treating and / or relieving pain. The amino acid sequence of the oligopeptide disclosed by the invention comprises a derivative sequence formed by additionally arranging 1-3 amino acids at the N end and / or C end of the amino acid sequence shown as Tyr-Pro-Arg, especially Tyr-Pro-Arg-Arg (YPRR, YR4), and a gastrointestinal tract digestion product Tyr-Pro-Arg (YPR, YR3) of the oligopeptide. The oligopeptide not only can relieve acute pain induced by a mouse thermal drifting model, but also can relieve chronic pain induced by a rat sciatic nerve chronic compression injury model and a tibia bone cancer pain model, and meanwhile, the oligopeptide obviously inhibits CCI-induced spinal astrocyte, microglia and neuron activation; therefore, the compound has important development and application values in the aspects of central sensitization inhibition and analgesia.

The present invention belongs to the technology of fermented milk, and particularly relates to a composite freeze-drying protective agent for probiotics, freeze-dried bacterial powder, fermented milk and uses thereof. The probiotic is Streptococcus thermophilus JM905, and the composite freeze-drying protective agent is composed of 3-3.5 parts by weight of arabic gum, 1-2 parts by weight of manganese sulfate, 2-3 parts by weight of maltodextrin, and 2-4 parts by weight of L-sodium glutamate. The composite freeze-drying protective agent can significantly improve the freeze-drying survival rate of Streptococcus thermophilus JM905 and overcome the technical problem that this probiotic is difficult to tolerate the freeze-drying process. The freeze-dried bacterial powder of Streptococcus thermophilus JM905 prepared therefrom can maintain a high viable bacteria count. The fermented milk prepared by using the freeze-dried bacterial powder of Streptococcus thermophilus JM905 has high contents of free amino acids, protein and total nucleotides, and the digestibility is significantly improved, and the content of soluble protein after gastrointestinal digestion is increased.

The invention belongs to the technical field of microbial fermentation, and particularly relates to saccharomycetes SY and application thereof in preparation of a codonopsis pilosula fermentation product which is high in in-vivo and in-vitro bioavailability and capable of improving gastrointestinal motility. The codonopsis pilosula fermented product is prepared by fermenting codonopsis pilosula with saccharomycetes SY. The content of lobetyolin in the pilose asiabell root leavening prepared by fermenting pilose asiabell root through the saccharomycetes SY is high, in-vitro digestion experiments prove that the removal rate of lobetyolin, total flavonoids and superoxide anion free radicals and the bioaccessibility of the total reducing capacity are remarkably improved, and rat experiments find that the pilose asiabell root leavening can promote gastrointestinal motility and improve the bioavailability of the pilose asiabell root. Intestinal mucosa regeneration and barrier repair are promoted; the content of effective components such as luteolin 7-glucosid-3 '-xyloside capable of enhancing immune cell activity, regulating inflammatory factor balance, protecting cells from oxidative stress injury and improving gastrointestinal digestion and absorption functions and energy metabolism in serum is increased, and absorption of the effective components in blood is promoted.

The invention relates to the technical field of traditional Chinese medicine preparations, and particularly discloses a preparation method of a probiotic fermented traditional Chinese medicine preparation, which comprises the following steps: carrying out aerobic fermentation on part of traditional Chinese medicine raw materials to obtain aerobic fermentation liquor and aerobic fermentation residues; performing anaerobic fermentation on the other part of the traditional Chinese medicine raw materials to obtain anaerobic fermentation liquid and anaerobic fermentation residues; mixing the aerobic and anaerobic fermentation residues and carrying out solid-state fermentation to prepare functional mycoplasm; and mixing the aerobic and anaerobic fermentation broth with the functional mycoplasm to obtain the microbial inoculum. According to the preparation method, a segmented fermentation process is combined with a fermentation inducer compounded by tea polyphenol and yeast extract, so that the problems of poor taste and low utilization rate of active ingredients of a traditional Chinese medicine preparation are effectively solved. The probiotic fermented traditional Chinese medicine preparation prepared by the preparation method disclosed by the invention is mellow in taste, palatable in sourness and sweetness and high in utilization rate of active ingredients, has the effects of strengthening the spleen and stomach and promoting digestion and removing food stagnation, and can be used for remarkably improving the gastrointestinal function, effectively reducing gastric emptying hysteresis and improving the small intestine propulsion capability and the overall gastrointestinal digestion capability.

This invention belongs to the field of food nutrition and functional factors, specifically relating to a method for preparing highly immunomodulatory ovotransferrin (OVT) hydrolysates based on an adult gastrointestinal simulated digestive system. The method involves processing OVT using a simulated adult gastrointestinal tract to prepare OVT hydrolysates containing HTEGSTT, TTSY, and / or RTAGWVIPMG. This invention reveals that OVT exhibits enhanced immunomodulatory activity after gastrointestinal digestion and hydrolysis, and is more readily hydrolyzed in simulated adult digestive fluids than in infant digestive fluids. Comparative experiments show that nutritional intervention with OVT simulated digestive products from adults and infants can promote the production of short-chain fatty acids such as acetic acid, propionic acid, and butyric acid, effectively alleviate swelling and damage to the small intestinal mucosa caused by inflammation, significantly improve intestinal barrier immune function, and OVT IA exhibits higher intestinal immune activity than OVT IB.

The invention provides a digestion-assisting fermented beverage and a preparation method thereof, and relates to the technical field of functional beverages, and the IPC classification number belongs to A23L2. The digestion-assisting fermented beverage disclosed by the invention is prepared from hawthorn, emblic leafflower fruit, bighead atractylodes rhizome, dried orange peel, poria cocos, malt and liquorice through enzymolysis and fermentation. Wherein the hawthorn is used as a monarch drug for eliminating meat accumulation, promoting the circulation of qi and relieving flatulence; the rhizoma atractylodis macrocephalae has the effects of tonifying spleen, eliminating dampness and enhancing small intestine peristalsis, and is a ministerial drug; the dried orange peel and the poria cocos are used for clearing damp and regulating intestinal flora, and the malt is used for eliminating grain accumulation and activating amylase; and liquorice harmonizes and protects stomach. All the medicines are combined to achieve the effects of promoting digestion, eliminating stagnation, tonifying spleen, eliminating dampness, promoting the circulation of qi and regulating the middle warmer. Test results show that the fermented beverage not only has good antioxidant activity, but also has an excellent effect of promoting gastrointestinal digestion.

The application belongs to the field of bioactive peptides, and particularly relates to an egg white peptide sequence QVPLW having a skin damage alleviating effect and application thereof. Specifically, the egg white peptide is prepared by alkaline protease enzymolysis, and after in vitro simulation of gastrointestinal digestion, the sequence thereof is determined by mass spectrometry. The egg white peptide QVPLW can significantly alleviate mechanical damage of skin fibroblasts (HSF) caused by cell scratching. Meanwhile, the egg white peptide QVPLW can also promote the proliferation of HSF cells and the migration of HSF cells in a Transwell chamber. The application provides a new idea for deep processing of egg products and targeted preparation of high-activity egg white peptides, and provides a new perspective for the development of functional foods applied in the auxiliary treatment of skin damage.

The invention relates to the technical field of food science and biology, and particularly discloses a method for measuring taste characteristics of novel umami peptide in simulated gastrointestinal digestion of Xuanwei ham. The method comprises the following steps: sequentially carrying out in-vitro simulated digestion on an oral phase, a stomach phase and an intestinal phase on Xuanwei ham homogenate to obtain a digestion product, carrying out centrifugation, filtration and freeze-drying treatment, and carrying out gel filtration chromatography separation to obtain main elution peaks, preferably selecting components with the strongest umami activity. Peptide fragment sequences are identified by using nano-liter liquid chromatography-mass spectrometry, candidate peptide fragments are screened by combining molecular weight, amino acid residue composition and toxicity prediction, and a variety of machine learning models are further adopted to predict umami potential. And performing chemical synthesis on the optimized peptide, determining a threshold value by a taste dilution method, and completing sensory evaluation. According to the method, the novel flavor peptide which is free of bitter taste and has a flavor threshold lower than that of monosodium glutamate can be efficiently screened out, and a technical support is provided for flavor enhancement of functional flavoring agents and fermented meat products.

The invention belongs to the technical field of functional protein peptides, and particularly discloses a grass seed and cotton seed protein peptide-selenium nano-composite and a preparation method and application thereof.The method comprises the following steps that grass seeds and cotton seeds are extracted and degreased, and grass seed and cotton seed protein freeze-dried powder A is prepared; removing non-protein, fat and mineral substances from the cotton seed protein freeze-dried powder A to prepare cotton seed protein freeze-dried powder B; preparing the grass cottonseed protein peptide from the grass cottonseed protein freeze-dried powder B in a simulated gastrointestinal digestion environment; the grass seed and cotton seed protein peptide and sodium selenite are used as raw materials to prepare the grass seed and cotton seed protein peptide-selenium nano-composite. According to the grass seed and cotton seed protein peptide-selenium nano-composite and the preparation method and application thereof, grass seeds and cotton seeds are used as raw materials, and the prepared grass seed and cotton seed protein peptide-selenium nano-composite has the advantages of being high in absorption rate, high in safety and high in oxidation resistance; therefore, the organic selenium supplement can be widely applied to the field of future food or cosmetics as a high-efficiency dietary organic selenium supplement.

The invention belongs to the technical field of microbial fermentation, and particularly relates to lactobacillus gasseri KB24 and application thereof in preparation of cattle spleen peptide for improving spleen deficiency. The preparation method comprises the following steps: (1) pretreatment: removing envelopes of the cattle spleen, crushing, and homogenizing to prepare cattle spleen slurry; (2) enzymolysis treatment: adding a compound enzyme into the cattle spleen slurry for enzymolysis, and sterilizing after enzymolysis to obtain an enzymatic hydrolysate; (3) performing fermentation treatment: inoculating lactobacillus gasseri KB24 into the enzymatic hydrolysate, and performing fermentation to obtain fermentation liquor; and (4) carrying out solid-liquid separation, filtration, ultrafiltration membrane separation and freeze drying on the fermentation liquor to obtain the cattle spleen peptide. The cattle spleen peptide prepared by the method can improve the spleen deficiency symptom from the aspects of weight, holding power, exhaustive swimming time, gastrointestinal digestive function, energy substance metabolism and the like, and can be applied to products for improving spleen deficiency.

The invention belongs to the technical field of biological medicines, and provides an anti-epileptic stiff silkworm polypeptide as well as a preparation method and application thereof. The bombyx batryticatus polypeptide is a peptide fragment with an amino acid sequence as shown in SEQ ID No.1, and is RFAFPAWI (PP6). The method comprises the following steps: preparing a peptide extract in stiff silkworm by simulating gastrointestinal digestion, identifying peptide in stiff silkworm polypeptide by using Nano LC-MS / MS, and carrying out difference analysis; differential peptides with potential anti-epileptic activity are obtained through virtual screening, and the anti-epileptic stiff silkworm polypeptide PP6 with potential anti-epileptic activity is synthesized. PP6 has a neuroprotection effect on PC12 cell neurotoxicity damaged by glutamic acid (Glu) by adjusting a GABA signal channel, and has an anti-epileptic effect on an epileptic mouse induced by pentaerythrityl tetrazole (PTZ). Therefore, PP6 may be a new leading peptide compound for developing epileptic drugs, and the anti-epileptic polypeptide can be widely applied to the fields of medicines, foods and the like to achieve the anti-epileptic target.

The invention relates to the technical field of food, in particular to an azolla filliculoidas milk double-protein solid beverage which mainly comprises azolla filliculoidas and whole milk powder. The rumex hanus is a nutritional supplement based on plants, contains a large amount of proteins, amino acids, vitamins, minerals and various plant compounds, and has the effects of improving gastrointestinal digestion, resisting oxidation, reducing blood sugar and blood pressure and improving immunity. The whole milk powder is rich in nutrition and strong in milk flavor and has the effects of sleeping peacefully, supplementing calcium and the like.

The application discloses a betalain-loaded chitosan oligosaccharide-sodium alginate hydrogel bead, and belongs to the technical field of pharmaceutical preparations. The betalain-chitosan oligosaccharide-sodium alginate hydrogel bead (Bt-COS-SA) is prepared by adopting an ionic gel method with sodium alginate and chitosan oligosaccharide as carriers, and the optimal preparation conditions of the hydrogel bead are as follows: the betalain concentration is 2 mg / mL, the sodium alginate concentration is 1.5%, the chitosan oligosaccharide concentration is 0.75%, and the CaCl2 concentration is 1.5%. Under the conditions, the embedding rate of the hydrogel bead to the betalain is 74.33+ / -2.31%. The in-vitro simulation gastrointestinal digestion experiment shows that the hydrogel bead has certain pH sensitivity and good sustained-release property; meanwhile, the antioxidation result shows that the antioxidation capacity of the betalain-loaded hydrogel bead is improved to a certain extent, and the hydrogel bead exhibits good application potential in the active substance delivery field.