22results about How to "Improve hydrolysis efficiency" patented technology

The invention discloses a process method for preparing fish collagen tripeptide and application of the fish collagen tripeptide, and belongs to the technical field of collagen peptide preparation processes. According to the method, the raw material fish skin is subjected to pretreatment and pretreatment, and then the pretreated fish skin is subjected to two times of enzymolysis treatment, so that efficient hydrolysis of fish skin collagen is realized, and a collagen peptide product with small molecular weight is obtained. Firstly, neutral protease and papain are used for carrying out primary enzymolysis on fish skin, then a microbial fermentation agent and an activity accelerant are used for carrying out deep fermentation enzymolysis treatment, peptide fragments can be effectively further cut, the yield of small molecule peptide fragments is increased, and the biological activity is improved. The method is simple, the process is easy to control, environmental pollution is avoided, the enzymolysis time is short, the enzymolysis speed and the extraction rate of the collagen peptide can be increased, and the collagen peptide obtained through the method is low in molecular weight, good in biological activity and easier to popularize and apply.

The invention belongs to the technical field of starch-based adhesive materials, and provides a boiling-water-resistant flame-retardant starch-based wood adhesive as well as a preparation method and application thereof. The preparation method comprises the following steps: mixing starch, water and alpha-amylase, and performing hydrolysis reaction to obtain a starch hydrolysate; mixing the starch hydrolysate and the DIHP aqueous solution, and reacting to obtain an S-DIHP material; and mixing the nano hybrid DE (at) TA with an S-DIHP material to obtain the boiling water-resistant flame-retardant starch-based wood adhesive. The adhesive provided by the invention is prepared through an enzyme catalysis hydrolysis strategy and a hyperbranched cross-linked network strategy, and has excellent boiling water resistance; by adding the nano hybrid DE (at) TA taking polysiloxane as a core and tannic acid as a shell, not only is the adhesive property of the adhesive improved, but also the flame retardance of the adhesive is remarkably enhanced, and the adhesive is suitable for the wood processing industry and is used for replacing a traditional petroleum-based adhesive, so that the environmental pollution is reduced.

The invention discloses a preparation method and application of hydrolyzed soybean meal peptide-enriched zinc. The preparation method comprises the following steps: crushing peeled soybean meal, adding water, adjusting the pH value to be neutral, and adding protease for hydrolysis; wherein the adding proportion of the protease is alpha-galactosidase accounting for 0.08%-0.1% of the weight of the soybean meal, papain accounting for 0.15%-0.2% of the weight of the soybean meal, neutral protease accounting for 0.15%-0.2% of the weight of the soybean meal and flavor enzyme accounting for 0.05%-0.1% of the weight of the soybean meal, enzyme inactivation treatment is carried out after complete hydrolysis, then the pH value of the system is adjusted to 4-6, soluble zinc salt is added, and the hydrolyzed soybean meal peptide-enriched zinc is obtained after reaction. The compound enzyme is used for hydrolysis, so that the hydrolysis efficiency is greatly improved, the content of acid-soluble protein is increased, the acid-soluble protein is chelated with a zinc source, an optimized hydrolyzed peptide-enriched zinc product is finally obtained, and the problem of low acid-soluble protein content of the fermented soybean meal at present is solved.

The utility model discloses hydrolysis equipment for producing fluorobenzoyl chloride, which relates to the field of hydrolysis equipment, and adopts the technical scheme that the hydrolysis equipment comprises a hydrolysis tank body, a top cover positioned at the top of the hydrolysis tank body, a bearing bracket which is arranged at the bottom of the hydrolysis tank body and is used for supporting the hydrolysis tank body, and a circulating assembly, the shell is connected with the bottom of the top cover through a vertical rod, one side of the shell is communicated and connected with a water inlet pipe driven by a water pump, the bottom of the shell is communicated and connected with a water outlet pipe, the water pump acts on the water inlet pipe to suck water in a hydrolysis tank body into the shell, and then the water is discharged through the water outlet pipe and enters the hydrolysis tank body, so that circulation is formed. A contact cavity for placing a solid catalyst is formed in the shell, and a sieve plate is arranged between the contact cavity and the water outlet pipe; the device has the effects that the catalyst can be circulated and fully contacted with a product formed by chlorine chlorination and water, so that the hydrolysis efficiency is greatly improved.

The present invention discloses a keratinase variant that improves hydrolysis ability by fusing a substrate binding domain, and belongs to the field of genetic engineering and enzyme engineering technology. The present invention uses PCR and Gibson connection technology to successfully fuse and express a substrate binding domain from Trichoderma reesei at the C-terminus of keratinase KerZ1 through a connecting peptide to obtain a keratinase variant KerZ1 / CBM‑L8. The enzyme activity assay results show that the enzyme activity of the keratinase variant KerZ1 / CBM‑L8 is only 41.5% of that of KerZ1, but the feather hydrolysis experiment results show that the average enzyme activity of the keratinase variant KerZ1 / CBM‑L8 is 2.71 times that of KerZ1, which has more application value and potential.

This invention belongs to the field of hard carbon technology, specifically relating to a bamboo-based and lignin-based hard carbon composite material, its preparation method, and its applications. The process involves pretreating raw materials to obtain pretreated material; pretreating the pretreated material with an acidic oxidant to obtain acidic oxidant pretreated material; pre-oxidizing the acidic oxidant pretreated material to obtain pre-oxidized material; pre-carbonizing the pre-oxidized material under a protective atmosphere to obtain pre-carbonized material; pulverizing the pre-carbonized material to obtain pulverized material; acid washing followed by water washing to obtain purified material; soaking the purified material in acid, filtering, and drying to obtain soaked material; mixing the soaked material with a modifier, and high-temperature coating and carbonizing under a protective atmosphere to obtain bamboo-based coated hard carbon or lignin-based coated hard carbon. Mixing the two types of hard carbon and carbon coating yields a bamboo-based and lignin-based hard carbon composite material, which is used in negative electrode sheets and batteries. When applied to batteries, the hard carbon composite material of this invention can improve battery energy density.

The utility model discloses hydrolysis reaction equipment for high-purity aluminum hydroxide production, which comprises a placing box, a hydrolysis cup is clamped in the placing box, electric sliding rails are fixedly mounted on two sides of the outer wall of the placing box, U-shaped frames are fixedly mounted at sliding ends of the two electric sliding rails, and the U-shaped frames are fixedly mounted on the U-shaped frames. An electric push rod is fixedly mounted in the middle of the U-shaped frame, and a stirring assembly is fixedly mounted at the telescopic end of the electric push rod. The hydrolysis cup is clamped in the placing box, the placing box is filled with low-temperature water, heat generated by hydrolysis of high-purity aluminum hydroxide can be dissipated, the surface temperature of the hydrolysis cup is prevented from being too high, the hydrolysis stability is improved, and through cooperation of a rotating base, a second driving motor and a gear, the hydrolysis cup can be rotated conveniently and rapidly. The reciprocating swing of the arc-shaped clamping block can be realized, the reciprocating swing of the hydrolysis cup is driven through the reciprocating swing of the arc-shaped clamping block, and the hydrolysis efficiency is effectively improved through the stirring of the stirring assembly.

The utility model relates to the technical field of organism hydrolysis, in particular to equipment with an organism hydrolysis treatment function, which comprises a rack, universal wheels and a hydrolysis bin, the rack is of a rectangular frame structure, and universal wheels are fixedly installed at the bottom of the rack. A power distribution cabinet is fixedly mounted on the outer wall of one end of the rack, and a hydrolysis bin is mounted in the rack in an embedded manner; a bin cover is mounted above the hydrolysis bin, the bin cover is movably connected with the rack, and the bin cover is fixed through a quick clamp; a driving motor is fixedly mounted at the top end of the hydrolysis bin; by improving equipment with an organism hydrolysis treatment function, the equipment has the advantages that the structural design is reasonable, the hydrolysis efficiency of organisms is improved in manners of stirring, aeration, heating and the like, harmful gas generated by hydrolysis is conveniently treated, and the utilization rate of an internal space is high; therefore, the problems and defects in the prior art and equipment are effectively solved.

The application discloses a method for efficiently extracting heparin sodium from biological materials, and the method comprises the following steps: cleaning and crushing the raw material at low temperature; adding poloxamer, ammonium sulfate and complex enzymes to perform synergistic enzymolysis under mild and neutral conditions; after the reaction is completed, the temperature is increased to above the cloud point of the polymer, liquid-liquid two-phase separation is triggered, fat-soluble impurities are synchronously removed, and the water phase rich in heparin sodium is collected; after the water phase is purified through ion exchange, concentration, alcohol precipitation and vacuum drying are sequentially performed, then vibration screening and low-temperature drying are performed, and finally, high-purity heparin sodium powder is obtained. Through the intelligent phase change characteristics of the temperature-sensitive polymer, the high-efficiency enzymolysis and primary purification are coupled, the yield and purity of heparin are significantly improved, the high-salt, strong-alkali or high-temperature conditions in the traditional process are avoided, the biological activity of the product is effectively protected, and the yield is improved.

This invention discloses a method for improving methanogenesis from lignocellulose based on a mixed-culture fermentation strategy, belonging to the field of anaerobic fermentation technology. The method includes the following steps: (1) inoculating activated *Trichoderma echinosporum* into a culture medium containing lignocellulose and loofah sponge as a biological carrier material for fermentation to obtain a fermentation broth; (2) activating activated sludge using a revitalizing solution; (3) inoculating the activated sludge from step (2) into the fermentation broth of *Trichoderma echinosporum* from step (1) for anaerobic fermentation to produce methanogens. This method achieves a methane yield of 262 mL / g VS, which is more than 30% higher than that of traditional anaerobic fermentation for methanogenesis, and is of great significance for improving the bioconversion of lignocellulose resources.

The invention relates to the technical field of lipoic acid preparation devices, and provides a lipoic acid ethyl ester hydrolysate acidification device which comprises a reaction kettle, a dispersing mechanism and an oscillating mechanism, the top of the reaction kettle is communicated with a liquid conveying pipe and a liquid inlet pipe, an atomizer is arranged at the end, located in the reaction kettle, of the liquid conveying pipe, a liquid outlet pipe is arranged at the bottom of the reaction kettle, and a valve is arranged on the liquid outlet pipe; a plurality of supporting foot stools are arranged at the bottom of the reaction kettle, a communicating pipe is arranged at the top of the reaction kettle, and a pressure gauge is arranged at one end of the communicating pipe away from the infusion pipe; according to the device, the mixed solution of the ethyl lipoate hydrolysate and the acidizing fluid is conveyed into the guide cylinder, and the plurality of through holes are formed in the outer side wall of the guide cylinder, so that the mixed solution of the ethyl lipoate hydrolysate and the acidizing fluid is continuously dispersed into the ethyl lipoate hydrolysate at different depths in the downward conveying process of the spiral auger; and an acidification environment is provided for rapid hydrolysis of the ethyl lipoate hydrolysate.

The invention discloses a self-assembly microsphere reactor for dairy product processing and a preparation method and application thereof. The preparation method comprises the following steps: preparing silicon dioxide colloid nanoparticles as a water phase and n-hexadecane as an oil phase, and preparing a receiving solution containing a surfactant; preparing a water-in-oil emulsion from the prepared oil phase and water phase, shearing the water-in-oil emulsion into liquid drops, and collecting the liquid drops into a receiving liquid containing a surfactant; evaporating a water phase in the formed liquid drops to obtain self-assembled silicon dioxide microspheres; and finally, filling the obtained self-assembled silicon dioxide microspheres into a quartz column to obtain the self-assembled silicon dioxide microspheres. The self-assembly microsphere reactor provides an efficient reactor model for lactase hydrolysis reaction, and has a promoting effect on improving lactase hydrolysis efficiency and stability; meanwhile, the risk of activity loss caused by lactase falling is reduced, and the reusability of a catalytic system is greatly enhanced.

The invention discloses a beta-lactamase immobilized sponge and a preparation method and application thereof, and belongs to the technical field of environmental engineering.Polydopamine coated melamine sponge is used as a carrier, beta-lactamase cross-linked enzyme aggregate is immobilized on the surface of the carrier through a cross-linking method, and the beta-lactamase immobilized sponge is prepared. The beta-lactamase immobilized sponge (MS (at) beta-lactamase-CLEAs) is obtained, so that the antibiotics in the wastewater can be efficiently removed. By adopting the method disclosed by the invention, the stability and the catalytic activity of the beta-lactamase are enhanced, and the MS-coated beta-lactamase-CLEAs disclosed by the invention can be used for quickly and efficiently hydrolyzing beta-lactam antibiotics in wastewater and remarkably reducing the concentration of the beta-lactam antibiotics; compared with a traditional treatment method, the method has higher treatment efficiency and good reusability, a feasible solution is provided for treatment of wastewater polluted by antibiotics, and the method has wide application prospects.

This invention discloses a method for detecting 3-chloro-1,2-propanediol esters and 2-chloro-1,3-propanediol esters in aquatic oils using liquid chromatography-tandem mass spectrometry (LC-MS / MS). The method employs porcine pancreatic lipase to specifically enzymatically hydrolyze esterified contaminants in krill oil, releasing free 3-chloro-1,2-propanediol and 2-chloro-1,3-propanediol. Derivatization is then performed using quinalyl chloride as a derivatizing agent. Combined with LC-MS / MS technology, this achieves high sensitivity and specificity for detection and effectively distinguishes the chromatographic behavior of these two isomers. By using mild and highly specific enzymatic hydrolysis instead of harsh chemical hydrolysis, and combining it with LC-MS / MS detection, the destruction of target analytes and side reactions during pretreatment are effectively reduced. The optimized pretreatment process improves the stability, accuracy, and detection efficiency of the method in complex lipid matrices, providing a reliable analytical tool for risk assessment and quality control of chloropropanol ester contaminants in aquatic oils and related oil products.

An efficient continuous hydrolysis tower comprises a tower body arranged outside, a feeding pipe arranged at the bottom of one side of the tower body, a backflow pipe arranged at the top of one side of the tower body, a first spraying disc and a second spraying disc which are installed inside the tower body, a water conveying pipe, a control assembly and a circulation assembly. Water conveying pipes connected with an external water pump are arranged at the bottoms of the first spraying disc and the second spraying disc, a control assembly is arranged at the top of the tower body, and a circulating assembly is arranged at the bottom of the inner side of the tower body; the control assembly comprises a mounting plate, a disc, an inner disc and a round hole, the mounting plate is fixed at the top end of the tower body, a driving motor is mounted on one side of the mounting plate, a driving gear is mounted at the top end of the driving motor, a rotating shaft is mounted in the middle of the tower body through a bearing, and a driven gear is mounted at the top end of the rotating shaft. The material hydrolysis efficiency is high, the hydrolysis degree is improved, the hydrolysis uniformity is good, meanwhile, the gas flow rate can be controlled, and use is facilitated.

The invention provides a preparation method of a yak oligopeptide powder product with an Alzheimer's disease intervention effect, and belongs to the technical field of biology.The yak oligopeptide powder product contains peptide YP, and the peptide YP is composed of leucine-glutamic acid-tyrosine-glycine, the peptide YP has relatively strong binding affinity with acetylcholin esterase, the docking binding energy is-9.5 kcal / mol, the peptide YP and residues of Tyr124, Gly121, Phe338, Asp74, His447, Ser203 and Glu202 of the acetylcholin esterase form 9 hydrogen bonds, and two electrostatic interactions and three hydrophobic interactions exist, so that the function of regulating the enzymatic activity is achieved. And a more reliable natural choice can be provided for intervention of the Alzheimer's disease.

The application discloses a system and method for resourceful and collaborative treatment of domestic sewage and kitchen waste, which is provided with an organically connected enhanced pretreatment unit, a carbon and phosphorus recovery unit, an enhanced biological treatment unit, a deep treatment unit and an energy recovery unit, is based on nitrification and denitrification denitrification treatment, is supplemented by deep treatment of pollutants, efficiently recovers carbon and phosphorus at the front end, adopts a water source heat pump and cogeneration at the tail end to realize energy recycling, deeply treats pollutants of the domestic sewage, outputs high-quality recycled water, collaboratively treats part of the kitchen waste, maximally recycles and utilizes energy and resources in the domestic sewage and the kitchen waste, reduces operation cost of a sewage plant, and contributes to carbon emission reduction and phosphorus crisis solving.

The invention relates to environment-friendly treatment of poultry manure, in particular to a process for stabilizing an anaerobic fermentation system, which comprises the following steps: fully collecting manure with ts concentration of 6%, performing anaerobic fermentation, improving the variety of antibiotics, promoting the activity of a hydrogen-philic methanogen flora, and improving an electrode material of a microbial electrolysis cell, the obtained anaerobic fermentation system is high in stability and rich in methane yield.

The invention relates to the technical field of biotechnology and ethanol fermentation, in particular to a method for improving corn ethanol yield by enhancing corn fiber enzymolysis saccharification. The method comprises the following steps: step 1, liquefying; step 2, saccharification: adjusting the pH value of the liquefied mash to 5 + / -0.2, then adding a saccharifying enzyme and a cellulase system, and saccharifying to obtain saccharified mash; step 3, synchronous saccharification and fermentation: activated yeast is added into the saccharified mash, after saccharification and fermentation, a cellulase system is added again, and then saccharification and fermentation are continued; and step 4, ethanol distillation. According to the method, the enzymolysis efficiency of corn fibers in the whole enzymolysis saccharification and fermentation process is enhanced, the concentration of ethanol produced by fermentation is high, the content of protein in the distillers' grains obtained after distillation solid-liquid separation is higher, and the yield of corn ethanol is increased.

The invention discloses a method for preparing a high-purity fish skin collagen tripeptide, and belongs to the field of food technology. The present invention is to solve the problems of large usage of composite enzymes, residual salt and free amino acids in the product, and low yield and purity of collagen tripeptides in the existing collagen tripeptide production process, and proposes to use composite enzyme ladder enzymolysis and ultrafiltration membrane to jointly prepare small molecule fish skin collagen peptides. Specifically, by neutral protease and papain ladder enzymolysis, the collagenase hydrolysis efficiency is maximized, and then papain that can specifically hydrolyze glycine (Gly) end is used to deeply hydrolyze 300-1000Da oligopeptides to improve the yield of collagen tripeptides; ultrafiltration and nanofiltration membrane combined separation technology are used to further purify collagen peptides, and free amino acids and peptides with molecular weight greater than 700Da are removed to improve collagen tripeptide (about 300Da) purity.

The invention discloses a production line and method for preparing glycollic acid through glycolate hydrolysis, and the production line comprises a mixing kettle used for mixing and heating glycolate and water; a rectifying tower; a heat end inlet of the heat exchanger is connected with the methanol steam outlet; a circulating outlet of the concentration kettle is connected with a cold end inlet of the heat exchanger, a circulating inlet is connected with a cold end outlet, and the concentration kettle is used for heating and concentrating the glycollic acid aqueous solution to obtain a high-concentration glycollic acid aqueous solution; the cooling kettle is used for carrying out gradient cooling crystallization on the high-concentration glycollic acid aqueous solution; a centrifuge; a dryer; and the impurity treatment tank is used for carrying out impurity treatment on the glycollic acid mother liquor. By optimizing the rectification temperature and pressure, the methanol water vapor obtained by rectification can meet the heating concentration requirement of the glycollic acid solution, and the energy consumption is remarkably reduced; glycollic acid mother liquor is recycled, so that the energy consumption of the device is reduced and the yield is increased; impurities in the glycollic acid mother liquor are treated and reused, so that the product purity is improved.

The application provides a method for preparing fish skin collagen peptides with high antioxidant activity by DHPM and UIO-66 molecular imprinting, and relates to the technical fields of aquatic product deep processing and bioactive peptides. The method comprises the following steps: fish skin pretreatment, DHPM-induced collagen protein unfolding, endogenous enzyme autolysis, crude separation, selective target enrichment by using UIO-66 material with histidine as a molecular imprinting template, and drying collection. The application destroys the dense triple helix structure of collagen protein by using the physical field effect of dynamic high pressure microfluidization, exposes the enzyme cutting site, uses the endogenous enzyme of fish skin for autolysis, and then uses the histidine molecular imprinting UIO-66 material to accurately capture the high antioxidant activity peptide segment, so that the enzymolysis efficiency and the antioxidant activity of the product are significantly improved. The application does not need to add exogenous enzymes, and has the advantages of green process, low cost and high product purity. The obtained fish skin collagen peptides can be widely applied to the fields of functional food, cosmetics and medicine.