7results about How to "Improve hydrolysis efficiency" patented technology

This invention belongs to the field of hard carbon technology, specifically relating to a bamboo-based and lignin-based hard carbon composite material, its preparation method, and its applications. The process involves pretreating raw materials to obtain pretreated material; pretreating the pretreated material with an acidic oxidant to obtain acidic oxidant pretreated material; pre-oxidizing the acidic oxidant pretreated material to obtain pre-oxidized material; pre-carbonizing the pre-oxidized material under a protective atmosphere to obtain pre-carbonized material; pulverizing the pre-carbonized material to obtain pulverized material; acid washing followed by water washing to obtain purified material; soaking the purified material in acid, filtering, and drying to obtain soaked material; mixing the soaked material with a modifier, and high-temperature coating and carbonizing under a protective atmosphere to obtain bamboo-based coated hard carbon or lignin-based coated hard carbon. Mixing the two types of hard carbon and carbon coating yields a bamboo-based and lignin-based hard carbon composite material, which is used in negative electrode sheets and batteries. When applied to batteries, the hard carbon composite material of this invention can improve battery energy density.

The application discloses a method for efficiently extracting heparin sodium from biological materials, and the method comprises the following steps: cleaning and crushing the raw material at low temperature; adding poloxamer, ammonium sulfate and complex enzymes to perform synergistic enzymolysis under mild and neutral conditions; after the reaction is completed, the temperature is increased to above the cloud point of the polymer, liquid-liquid two-phase separation is triggered, fat-soluble impurities are synchronously removed, and the water phase rich in heparin sodium is collected; after the water phase is purified through ion exchange, concentration, alcohol precipitation and vacuum drying are sequentially performed, then vibration screening and low-temperature drying are performed, and finally, high-purity heparin sodium powder is obtained. Through the intelligent phase change characteristics of the temperature-sensitive polymer, the high-efficiency enzymolysis and primary purification are coupled, the yield and purity of heparin are significantly improved, the high-salt, strong-alkali or high-temperature conditions in the traditional process are avoided, the biological activity of the product is effectively protected, and the yield is improved.

This invention discloses a method for improving methanogenesis from lignocellulose based on a mixed-culture fermentation strategy, belonging to the field of anaerobic fermentation technology. The method includes the following steps: (1) inoculating activated *Trichoderma echinosporum* into a culture medium containing lignocellulose and loofah sponge as a biological carrier material for fermentation to obtain a fermentation broth; (2) activating activated sludge using a revitalizing solution; (3) inoculating the activated sludge from step (2) into the fermentation broth of *Trichoderma echinosporum* from step (1) for anaerobic fermentation to produce methanogens. This method achieves a methane yield of 262 mL / g VS, which is more than 30% higher than that of traditional anaerobic fermentation for methanogenesis, and is of great significance for improving the bioconversion of lignocellulose resources.

This invention discloses a method for detecting 3-chloro-1,2-propanediol esters and 2-chloro-1,3-propanediol esters in aquatic oils using liquid chromatography-tandem mass spectrometry (LC-MS / MS). The method employs porcine pancreatic lipase to specifically enzymatically hydrolyze esterified contaminants in krill oil, releasing free 3-chloro-1,2-propanediol and 2-chloro-1,3-propanediol. Derivatization is then performed using quinalyl chloride as a derivatizing agent. Combined with LC-MS / MS technology, this achieves high sensitivity and specificity for detection and effectively distinguishes the chromatographic behavior of these two isomers. By using mild and highly specific enzymatic hydrolysis instead of harsh chemical hydrolysis, and combining it with LC-MS / MS detection, the destruction of target analytes and side reactions during pretreatment are effectively reduced. The optimized pretreatment process improves the stability, accuracy, and detection efficiency of the method in complex lipid matrices, providing a reliable analytical tool for risk assessment and quality control of chloropropanol ester contaminants in aquatic oils and related oil products.

The application discloses a system and method for resourceful and collaborative treatment of domestic sewage and kitchen waste, which is provided with an organically connected enhanced pretreatment unit, a carbon and phosphorus recovery unit, an enhanced biological treatment unit, a deep treatment unit and an energy recovery unit, is based on nitrification and denitrification denitrification treatment, is supplemented by deep treatment of pollutants, efficiently recovers carbon and phosphorus at the front end, adopts a water source heat pump and cogeneration at the tail end to realize energy recycling, deeply treats pollutants of the domestic sewage, outputs high-quality recycled water, collaboratively treats part of the kitchen waste, maximally recycles and utilizes energy and resources in the domestic sewage and the kitchen waste, reduces operation cost of a sewage plant, and contributes to carbon emission reduction and phosphorus crisis solving.

The invention relates to the technical field of biotechnology and ethanol fermentation, in particular to a method for improving corn ethanol yield by enhancing corn fiber enzymolysis saccharification. The method comprises the following steps: step 1, liquefying; step 2, saccharification: adjusting the pH value of the liquefied mash to 5 + / -0.2, then adding a saccharifying enzyme and a cellulase system, and saccharifying to obtain saccharified mash; step 3, synchronous saccharification and fermentation: activated yeast is added into the saccharified mash, after saccharification and fermentation, a cellulase system is added again, and then saccharification and fermentation are continued; and step 4, ethanol distillation. According to the method, the enzymolysis efficiency of corn fibers in the whole enzymolysis saccharification and fermentation process is enhanced, the concentration of ethanol produced by fermentation is high, the content of protein in the distillers' grains obtained after distillation solid-liquid separation is higher, and the yield of corn ethanol is increased.

The application provides a method for preparing fish skin collagen peptides with high antioxidant activity by DHPM and UIO-66 molecular imprinting, and relates to the technical fields of aquatic product deep processing and bioactive peptides. The method comprises the following steps: fish skin pretreatment, DHPM-induced collagen protein unfolding, endogenous enzyme autolysis, crude separation, selective target enrichment by using UIO-66 material with histidine as a molecular imprinting template, and drying collection. The application destroys the dense triple helix structure of collagen protein by using the physical field effect of dynamic high pressure microfluidization, exposes the enzyme cutting site, uses the endogenous enzyme of fish skin for autolysis, and then uses the histidine molecular imprinting UIO-66 material to accurately capture the high antioxidant activity peptide segment, so that the enzymolysis efficiency and the antioxidant activity of the product are significantly improved. The application does not need to add exogenous enzymes, and has the advantages of green process, low cost and high product purity. The obtained fish skin collagen peptides can be widely applied to the fields of functional food, cosmetics and medicine.