Method for promoting hydrolysis of lignocellulose

Abstract

The invention discloses a method for promoting hydrolysis of lignocelluloses. According to the method, metal ions such as nickel, cobalt, magnesium, zinc, manganese and the like are added in the processes of saccharifying and hydrolyzing the lignocelluloses so as to improve the enzyme activity of the ligno-cellulase and hemicellulase, thereby further improving the hydrolysis efficiency of the ligno-cellulase. Proved by experiments, the hydrolysis efficiency of glucan can be improved by 4.5 times by adding the metal ions. According to the method, the operation is simple, the metal ions can be recycled, the investment cost is low, the usage range is wide, the pollution does not exist, the hydrolysis efficiency of the cellulose is obviously improved, and the method can be widely applied in the process of hydrolyzing the lignocelluloses and in the fields of manufacturing and developing biomass energy.

Description

technical field

[0001] The invention relates to a method for promoting the hydrolysis of lignocellulose, which belongs to the fields of biotechnology and biochemical industry. Background technique

[0002] As we all know, the EU cut subsidies for biofuels on September 18, 2012, clearly pointing out that biodiesel, a biofuel, and energy crops cannot be used as a long-term development route for EU biofuels in terms of carbon dioxide emissions and sustainable development, and can only be added in a limited way. 5% environmentally friendly ethanol derived from food, used to alleviate the energy crisis. However, due to the severe drought in many countries, international food prices have risen by 50% in a short period of time. How to replace petroleum with biofuel—lignocellulosic fuel without affecting food security—has once again become a global focus. Second-generation cellulosic ethanol uses lignocellulose with the largest reserves as the raw material, and is the development d...

Examples

Embodiment 1

[0031] Accurately weigh 0.81g of xylose residue (containing 5% dextran) into a 15ml centrifuge tube. Add 0.5 ml of Penicillium decumbens enzyme solution at 30 mg protein / g dextran. Then prepare a certain concentration of five metal ion solutions of nickel sulfate, cobalt chloride, magnesium chloride, zinc chloride, and manganese chloride, and use 0.05M sodium acetate buffer to make up the volume. The final concentrations of the five metal ion solutions are 1 and 10 mM concentrations of the two metal ions after filling the entire 10 ml saccharification system. The sodium acetate buffer without adding any metal ion solution was used as a control. Finally, saccharification was carried out at 45°C for 72 hours. Using HPLC to measure the glucose concentration, under the influence of 10mM zinc chloride solution, nickel sulfate solution and cobalt chloride solution, the glucose concentration increased from 1.3g / L to 5.46g / L, 6.13g / L and 6.33g / L respectively , see Table 1 for the s...

Embodiment 2

[0033] Accurately weigh 0.68g of delignified xylose residue (containing 5% dextran) into a 15ml centrifuge tube. Add 0.5 ml of Penicillium decumbens enzyme solution at 30 mg protein / g dextran. Then prepare a certain concentration of five metal ion solutions of nickel sulfate, cobalt chloride, magnesium chloride, zinc chloride, and manganese chloride, and use 0.05M sodium acetate buffer to make up the volume. The final concentrations of the five metal ion solutions are 1 and 10 mM concentrations of the two metal ions after filling the entire 10 ml saccharification system. The sodium acetate buffer without adding any metal ion solution was used as a control. Finally, saccharification was carried out at 45°C for 72 hours. Using HPLC to measure glucose concentration, under the influence of zinc chloride, manganese chloride, nickel sulfate, cobalt chloride and other metal ion solutions, the hydrolysis efficiency has been improved accordingly. Wherein the 10mM cobalt chloride sol...

Embodiment 3

[0035] After the crushed corncobs were steam-exploded at 2.5MPa for 90s, accurately weigh 1.5g of corncob blasting powder (containing 5% dextran) in a 15ml centrifuge tube, and add Xiasheng cellulase and Unocal Xylanase. Prepare a certain concentration of nickel sulfate, cobalt chloride, magnesium chloride, zinc chloride, and manganese chloride five metal ion solutions, use 0.05M sodium acetate buffer solution to make up to 10mL, and carry out saccharification in a shaking water bath at 45°C for 72 hours. . Using HPLC to measure the concentration of glucose and xylose, under the influence of zinc chloride, manganese chloride, nickel sulfate, cobalt chloride and other metal ion solutions, the hydrolysis efficiency has a corresponding increase, and the improvement efficiency of glucose is higher than that of xylose. The specific experimental results are shown in Table 3.

[0036] See Table 4 for the relative percentages of hydrolyzed monosaccharides in different concentrations...

technical field

[0001] The invention relates to a method for promoting the hydrolysis of lignocellulose, which belongs to the fields of biotechnology and biochemical industry. Background technique

[0002] As we all know, the EU cut subsidies for biofuels on September 18, 2012, clearly pointing out that biodiesel, a biofuel, and energy crops cannot be used as a long-term development route for EU biofuels in terms of carbon dioxide emissions and sustainable development, and can only be added in a limited way. 5% environmentally friendly ethanol derived from food, used to alleviate the energy crisis. However, due to the severe drought in many countries, international food prices have risen by 50% in a short period of time. How to replace petroleum with biofuel—lignocellulosic fuel without affecting food security—has once again become a global focus. Second-generation cellulosic ethanol uses lignocellulose with the largest reserves as the raw material, and is the development d...

Examples

Embodiment 1

[0031] Accurately weigh 0.81g of xylose residue (containing 5% dextran) into a 15ml centrifuge tube. Add 0.5 ml of Penicillium decumbens enzyme solution at 30 mg protein / g dextran. Then prepare a certain concentration of five metal ion solutions of nickel sulfate, cobalt chloride, magnesium chloride, zinc chloride, and manganese chloride, and use 0.05M sodium acetate buffer to make up the volume. The final concentrations of the five metal ion solutions are 1 and 10 mM concentrations of the two metal ions after filling the entire 10 ml saccharification system. The sodium acetate buffer without adding any metal ion solution was used as a control. Finally, saccharification was carried out at 45°C for 72 hours. Using HPLC to measure the glucose concentration, under the influence of 10mM zinc chloride solution, nickel sulfate solution and cobalt chloride solution, the glucose concentration increased from 1.3g / L to 5.46g / L, 6.13g / L and 6.33g / L respectively , see Table 1 for the s...

Embodiment 2

[0033] Accurately weigh 0.68g of delignified xylose residue (containing 5% dextran) into a 15ml centrifuge tube. Add 0.5 ml of Penicillium decumbens enzyme solution at 30 mg protein / g dextran. Then prepare a certain concentration of five metal ion solutions of nickel sulfate, cobalt chloride, magnesium chloride, zinc chloride, and manganese chloride, and use 0.05M sodium acetate buffer to make up the volume. The final concentrations of the five metal ion solutions are 1 and 10 mM concentrations of the two metal ions after filling the entire 10 ml saccharification system. The sodium acetate buffer without adding any metal ion solution was used as a control. Finally, saccharification was carried out at 45°C for 72 hours. Using HPLC to measure glucose concentration, under the influence of zinc chloride, manganese chloride, nickel sulfate, cobalt chloride and other metal ion solutions, the hydrolysis efficiency has been improved accordingly. Wherein the 10mM cobalt chloride sol...

Embodiment 3

[0035] After the crushed corncobs were steam-exploded at 2.5MPa for 90s, accurately weigh 1.5g of corncob blasting powder (containing 5% dextran) in a 15ml centrifuge tube, and add Xiasheng cellulase and Unocal Xylanase. Prepare a certain concentration of nickel sulfate, cobalt chloride, magnesium chloride, zinc chloride, and manganese chloride five metal ion solutions, use 0.05M sodium acetate buffer solution to make up to 10mL, and carry out saccharification in a shaking water bath at 45°C for 72 hours. . Using HPLC to measure the concentration of glucose and xylose, under the influence of zinc chloride, manganese chloride, nickel sulfate, cobalt chloride and other metal ion solutions, the hydrolysis efficiency has a corresponding increase, and the improvement efficiency of glucose is higher than that of xylose. The specific experimental results are shown in Table 3.

[0036] See Table 4 for the relative percentages of hydrolyzed monosaccharides in different concentrations...

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